Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,955,294 G→A 22.3% S1697S (AGC→AGT)  mnxG ← manganese‑oxidizing multicopper oxidase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,955,2940GA22.3% 74.4 / 15.7 41S1697S (AGC→AGT) mnxGmanganese‑oxidizing multicopper oxidase
Reads supporting (aligned to +/- strand):  ref base G (13/18);  new base A (5/4);  total (19/22)
Fisher's exact test for biased strand distribution p-value = 7.05e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01

CCGGACGACCATCGGCCCAGCGCATGTTCAGTGCGCGCTGCCACACCGTGACGAAGTCGCGGTAGTCCGGCTGGCCGCTGACCTTCACCGTGGCCTGGGTACGGGTAGCAGTATCCTCGGTCCAGGTGGCGCTTTGCGGCAGGATGCTCATGGCGCCCACCAGGCCTTTTTGCGGCTGCTTGATGAAGTCTGACGGGGTCAGGTTGAGGCCGCCGAACTCGATAGGCGTGGTGTTTATGTTGTCCACTGTGCGGCCCAGCTGC  >  NC_002947/3955149‑3955411
                                                                                                                                                 |                                                                                                                     
ccGGACGACCATCGGCCCAGCGCATGTTCAGTGCGCGCTGCCACACCGTGACGAAGTCGCGGTAGTCCGGCTGGCCGCTGACCTTCACCGTGGCCTGGGTACGGGTAGCAGTATCCTCGGTCCAGGTGGCGCTTTGCGGCAGGATACTc                                                                                                                    <  2:257073/149‑1 (MQ=255)
ccGGACGACCATCGGCCCAGCGCATGTTCAGTGCGCGCTGCCACACCGTGACGAAGTCGCGGTAGTCCGGCCGGCCGCTGACCTTCACCGTGGCCTGGGTACGGGTAGCAGTATCCTCGGTCCAGGTGGCGCTTTGCGGCAGGATGCt                                                                                                                     >  2:281787/1‑148 (MQ=255)
     cgacCATCGGCCCAGCGCATGTTCAGTGCGCGCTGCCACACCGTGACGAAGTCGCGGTAGTCCGGCTGGCCGCTGACCTTCACCGTGGCCTGGGTACGGGTAGCAGTATCCTCGGTCCAGGTGGCGCTTTGCGGCAGGATGCTCATGgc                                                                                                               <  2:213287/149‑1 (MQ=255)
             ggCCCAGCGCATGTTCAGTGCGCGCTGCCACACCGTGACGAAGTCGCGGTAGTCCGGCTGGCCGCTGACCTTCACCGTGGCCTGGGTACGGGTAGCAGTATCCTCGGTCCAGGTGGCGCTTTGCGGCAGGATGCTCATGGCGCccac                                                                                                         >  1:124748/1‑147 (MQ=255)
                ccAGCCCATGTTCAGTGCGCGCTGCCACACCGTGACGAAGTCGCGGTAGTCCGGCTGGCCGCTGACCTTCACCGTGGCCTGGGTACGGGTAGCAGTATCCTCGGTCCAGGTGGCGCTTTGCGGCAGGATGCTCATGGCGCCCACCATGc                                                                                                    >  2:93112/1‑149 (MQ=255)
                     gcATGTTCAGTGCGCGCTGCCACACCGTGACGAAGTCGCGGTAGTCCGGCTGGCCGCTGACCTTCACCGTGGCCTGGGTACGGGTAGCAGTATCCTCGGTCCAGGTGGCGCTTTGCGGCAGGATGCTCATgg                                                                                                                >  8:36355/1‑132 (MQ=255)
                     gcATGTTCAGTGCGCGCTGCCACACCGTGACGAAGTCGCGGTAGTCCGGCTGGCCGCTGACCTTCACCGTGGCCTGGGTACGGGTAGCAGTATCCTCGGTCCAGGTGGCGCTTTGCGGCAGGATGCTCATgg                                                                                                                <  7:36355/132‑1 (MQ=255)
                      cATGTTCAGTGCGCGCTGCCACACCGTGACGAAGTCGCGGTAGTCCGGCTGGCCGCTGACCTTCACCGTGGCCTGGGTACGGGTAGCAGTATCCTCGGTCCAGGTGGCGCTTTGCGGCAGGATACTCATGGCGCCCACCAGGCCttttt                                                                                              >  2:162580/1‑149 (MQ=255)
                            cAGTGCGCGCTGCCACACCGTGACGAAGTCGCGGTAGTCCGGCTGGCCGCTGACCTTCACCGTGGCCTGGGTACGGGTAGCAGTATCCTCGGTCCAGGTGGCGCTTTGCGGCAGGATGCTCATGGCGCCCACCAGGCCTTTTTGCGgct                                                                                        >  4:108486/1‑149 (MQ=255)
                               tGCGCGCTGCCACACCGTGACGAAGTCGCGGTAGTCCGGCTGGCCGCTGACCTTCACCGTGGCCTGGGTACGGGTAGCAGTATCCTCGGTCCAGGTGGCGCTTTGCGGCAGGATGCTCATGGCGCCCACCAGGCCTTTTTGCGgctgct                                                                                     >  6:52480/1‑149 (MQ=255)
                                   cgcTGCCACACCGTGACGAAGTCGCGGTAGTCCGGCTGGCCGCTGACCTTCACCGTGGCCTGGGTACGGGTAGCAGTATCCTCGGTCCAGGTGGCGCTTTGCGGCAGGATGCTCATGGCGCCCACCATGCCTTTTTGCGGCTGCTtgat                                                                                 <  1:93112/149‑1 (MQ=255)
                                   cgcTGCCACACCGTGACGAAGTCGCGGTAGTCCGGCTGGCCGCTGACCTTCACCGTGGCCTGGGTACGGGTAGCAGTATCCTCGGTCCAGGTGGCGCTTTGCGGCAGGATGCTCATGGCGCCCACCAGGCCTTTTTGCGGCTGCTtgat                                                                                 <  3:108486/149‑1 (MQ=255)
                                       gCCACACCGTGACGAAGTCGCGGTAGTCCGGCTGGCCGCTGACCTTCACCGTGGCCTGGGTATGGGTAGCAGTATCCTCGGTCCAGGTGGCGCTTTGCGGCAGGATACTCATGGCGCCCACCAGGCCTTTTTGCGGCTGCTTGATGAAg                                                                             >  1:326631/1‑149 (MQ=255)
                                        ccACACCGTGACGAAGTCGCGGTAGTCCGGCTGGCCGCTGACCTTCACCGTGGCCTGGGTACGGGTAGCAGTATCCTCGGTCCAGGTGGCGCTTTGCGGCAGGATGCTCATGGCGCCCACCAGGCCTTTTTGCGGCTGCTTGATGAAGt                                                                            <  6:162569/149‑1 (MQ=255)
                                        ccACACCGTGACGAAGTCGCGGTAGTCCGGCTGGCCGCTGACCTTCACCGTGGCCTGGGTACGGGTAGCAGTATCCTCGGTCCAGGTGGCGCTTTGCGGCAGGATACTCATGGCGCCCACCAGGCCTTTTTGCGGCTGCTTGATGAAGt                                                                            >  8:54996/1‑149 (MQ=255)
                                          acacCGTGACGAAGTCGCGGTAGTCCGGCTGGCCGCTGACCTTCACCGTGGCCTGGGTACGGGTAGCAGTATCCTCGGTCCAGGTGGCGCTTTGCGGCAGGATGCTCATGGCGCCCACCAGGCCTTTTTGCGGCTGCTTGATGAAGTCt                                                                          >  7:14970/1‑149 (MQ=255)
                                             ccGTGACGAAGTCGCGGTAGTCCGGCCGGCCGCTGACCTTCACCGTGGCCTGGGTACGGGTAGCAGTATCCTCGGTCCAGGTGGCGCTTTGCGGCAGGATGCTCATGGCGCCCACCAGGCCTTTTTGCGGCTGCTTGATGAAGTCTGAc                                                                       <  1:281787/149‑1 (MQ=255)
                                              cGTGACGAAGTCGCGGTAGTCCGGCTGGCCGCTGACCTTCACCGTGGCCTGGGTACGGGTAGCAGTATCCTCGGTCCAGGTGGCGCTTTGCGGCAGGATACTCATGGCGCCCACCAGGCCTTTTTGCGGCTGCTTGATGAAGTCTGACg                                                                      <  6:298920/149‑1 (MQ=255)
                                                tGACGAAGTCGCGGTAGTCCGGCTGGCCGCTGACCTTCACCGTGGCCTGGGTACGGGTAGCAGTATCCTCGGTCCAGGTGGCGCTTTGCGGCAGGATGCTCATGGCGCCCACCAGGCCTTTTTGCGGCTGCTTGATGAAGTCTGACggg                                                                    <  6:85751/149‑1 (MQ=255)
                                                    gAAGTCGCGGTAGTCCGGCTGGCCGCTGACCTTCACCGTGGCCTGGGTACGGGTAGCAGTATCCTCGGTCCAGGTGGCGCTTTGCGGCAGGATGCTCATGGCGCCCACCAGGCCTTTTTGCGGCTGCTTGATGAAGTCTGACGGGGTCa                                                                <  2:124748/149‑1 (MQ=255)
                                                           cgGTAGTCCGGCTGGCCGCTGACCTTCACCGTGGCCTGGGTATGGGTAGCAGTATCCTCGGTCCAGGTGGCGCTTTGCGGCAGGATACTCATGGCGCCCACCAGGCCTTTTTGCGGCTGCTTGATGAAGTCTGACGGGGTCAGGTTGAg                                                         <  2:326631/149‑1 (MQ=255)
                                                                gTCCGGCTGGCCGCTGACCTTCACCGTGGCCTGGGTACGGGTAGCAGTATCCTCGGTCCAGGTGGCGCTTTGCGGCAGGATGCTCATGGCGCCCACCAGGCCTTTTTGCGGCTGCTTGATGAAGTCTGACGGGGTCAGGTTGAGgccgc                                                    <  6:278320/149‑1 (MQ=255)
                                                                 tCCGGCTGGCCGCTGACCTTCACCGTGGCCTGGGTACGGGTAGCAGTATCCTCGGTCCAGGTGGCGCTTTGCGGCAGGATGCTCATGGCGCCCACCAGGCCTTTTTGCGGCTGCTTGATGAAGTCTGACGGGGTCAGGTTGAGgccgcc                                                   >  2:328344/1‑149 (MQ=255)
                                                                     gCTGGCCGCTGACCTTCACCGTGGCCTGGGTACGGGTAGCAGTATCCTCGGTCCAGGTGGCGCTTTGCGGCAGGATACTCATGGCGCCCACCAGGCCTTTTTGCGGCTGCTTGATGAAGTCTGACGGGGTCAGGTTGAGGCCGCCGAAc                                               >  8:133808/1‑149 (MQ=255)
                                                                           cGCTGACCTTCACCGTGGCCTGGGTACGGGTAGCAGTATCCTCGGTCCAGGTGGCGCTTTGCGGCAGGATGCTCATGGCGCCCACCAGGCCTTTTTGCGGCTGCTTGATGAAGTCTGACGGGGTCAGGTTGAGGCCGCCGAACTCGa                                           <  3:236094/147‑1 (MQ=255)
                                                                           cGCTGACCTTCACCGTGGCCTGGGTACGGGTAGCAGTATCCTCGGTCCAGGTGGCGCTTTGCGGCAGGATGCTCATGGCGCCCACCAGGCCTTTTTGCGGCTGCTTGATGAAGTCTGACGGGGTCAGGTTGAGGCCGCCGAACTCGa                                           >  6:366842/1‑147 (MQ=255)
                                                                           cGCTGACCTTCACCGTGGCCTGGGTACGGGTAGCAGTATCCTCGGTCCAGGTGGCGCTTTGCGGCAGGATGCTCATGGCGCCCACCAGGCCTTTTTGCGGCTGCTTGATGAAGTCTGACGGGGTCAGGTTGAGGCCGCCGAACTCGa                                           <  5:366842/147‑1 (MQ=255)
                                                                           cGCTGACCTTCAACGTCGCCTGGGTACCGGTAGAAGTATCCTCGGTCCAGGTGGCGCTTTGCAGCAGGATCCACATGGCACCCACCAGGCCTTTTTGCGACTGATTGATGAAGTCTGACGGGGTCAGTTTTAGGACTCCGAACTCGATa                                         >  4:236094/1‑149 (MQ=255)
                                                                                aCCTTCACCGTGGCCTGGGTACGGGTAGCAGTATCCTCGGTCCAGGTGGCGCTTTGCGGCAGGATGCTCATGGCGCCCCCCAGGCCTTTTTGCGGCTGCGTGATGATGTCTGGCGGGGTCAGGGTGAGGCCGCCGACGTCGATTGGCgt                                    >  4:304395/1‑149 (MQ=255)
                                                                                aCCTTCACCGTGGCCTGGGTACGGATAGCAGTATCCTCGGTCCAGGTGGCGCTTTGCGGCAGGATACTCATGGCCCCCACCAGGCCTTTTTGCGGCTGCTTGATGAAGTCTGACGGGGTCAGGTTGAGGCCGCCGAACTCGATAGGCgt                                    >  7:52396/1‑149 (MQ=255)
                                                                                 ccTTCACCGTGGCCTGGGTACGGGTAGCAGTATCCTCGGTCCAGGTGGCGCTTTGCGGCAGGATACTCATGGCGCCCACCAGGCCTTTTTGCGGCTGCTTGATGAAGTCTGACGGGGTCAGGTTGAGGCCGCCGAACTCGATAGGCgtg                                   <  1:162580/149‑1 (MQ=255)
                                                                                         gTGGCCTGGGTACGGGTAGCAGTATCCTCGGTCCAGGTGGCGCTTTGCGGCAGGATGCTCATGGCGCCCACCAGGCCTTTTTGCGGCTGCTTGATGAAGTCTGACGGGGTCAGGTTGAGGCCCCCGAACTCGATAGGCGTGGTGTTTAt                           <  2:20099/149‑1 (MQ=255)
                                                                                          gggCCGGGGTACGGGTAGCAGTATCCGCGGTCCTGGTGGCGCTTTGCGGCAGGATGCTCATGGCGCCCACCAGGCCTTTTTGCGGCTGCTTGATGAAGTCTGACGGGGTCAGGTTGAGGCCGCCGAACTCGATAGGCGTGGTGGTTAtg                          <  4:95322/148‑1 (MQ=255)
                                                                                              cTGGGTACGGGTAGCAGTATCCTCGGTCCAGGTGGCGCTTTGCGGCAGGATGCTCATGGCGCCCACCAGGCCTTTTTGCGGCTGCTTGATGAAGTCTGACGGGGTCAGGTTGAGGCCGCCGAACTCGATAGGCGTGGTGTTTAtgttgt                      <  5:52480/149‑1 (MQ=255)
                                                                                              cTGGGTACGGGTAGCAGTATCCTCGGTCCAGGTGGCGCTTTGCGGCAGGATGCTCATGGCGCCCACCAGGCCTTTTTGCGGCTGCTTGATGAAGTCTGACGGGGTCAGGTTGAGGCCGCCGAACTCGATAGGCGTGGTGTTTAtgttgt                      <  1:328344/149‑1 (MQ=255)
                                                                                                  gTACGGGTAGCAGTATCCTCGGTCCAGGTGGCGCTTTGCGGCAGGATGCTCATGGCGCCCACCAGGCCTTTTTGCGGCTGCTTGATGAAGTCTGACGGGGTCAGGTTGAGGCCGCCGAACTCGATAGGCGTGGTGTTTAtg                          >  6:248263/1‑141 (MQ=255)
                                                                                                  gTACGGGTAGCAGTATCCTCGGTCCAGGTGGCGCTTTGCGGCAGGATGCTCATGGCGCCCACCAGGCCTTTTTGCGGCTGCTTGATGAAGTCTGACGGGGTCAGGTTGAGGCCGCCGAACTCGATAGGCGTGGTGTTTAtg                          <  5:248263/141‑1 (MQ=255)
                                                                                                   tACGGGTAGCAGTATCCTCGGTCCAGGTGGCGCTTTGCGGCAGGATGCTCATGGCGCCCACCAGGCCTTTTTGCGGCTGCTTGATGAAGTCTGACGGGGTCAGGTTGAGGCCGCCGAACTCGATAg                                        >  7:218694/1‑126 (MQ=255)
                                                                                                   tACGGGTAGCAGTATCCTCGGTCCAGGTGGCGCTTTGCGGCAGGATGCTCATGGCGCCCACCAGGCCTTTTTGCGGCTGCTTGATGAAGTCTGACGGGGTCAGGTTGAGGCCGCCGAACTCGATAg                                        <  8:218694/126‑1 (MQ=255)
                                                                                                                   cTCGGTCCAGGTGGCGCTTTGCGGCAGGATGCTCATGGCGCCCACCAGGCCTTTTTGCGGCTGCTTGATGAAGTCTGACGGGGTCAGGTTGAGGCCGCCGAACTCGATAGGCGTGGTGTTTATGTTGTCCACTGTGCGGCCCAGCTGc  >  2:334096/1‑148 (MQ=255)
                                                                                                                   cTCGGTCCAGGTGGCGCTTTGCGGCAGGATGCTCATGGCGCCCACCAGGCCTTTTTGCGGCTGCTTGATGAAGTCTGACGGGGTCAGGTTGAGGCCGCCGAACTCGATAGGCGTGGTGTTTATGTTGTCCACTGTGCGGCCCAGCTGc  <  1:334096/148‑1 (MQ=255)
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CCGGACGACCATCGGCCCAGCGCATGTTCAGTGCGCGCTGCCACACCGTGACGAAGTCGCGGTAGTCCGGCTGGCCGCTGACCTTCACCGTGGCCTGGGTACGGGTAGCAGTATCCTCGGTCCAGGTGGCGCTTTGCGGCAGGATGCTCATGGCGCCCACCAGGCCTTTTTGCGGCTGCTTGATGAAGTCTGACGGGGTCAGGTTGAGGCCGCCGAACTCGATAGGCGTGGTGTTTATGTTGTCCACTGTGCGGCCCAGCTGC  >  NC_002947/3955149‑3955411

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑