| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_002947 | 1,499,508 | +C | intergenic (+85/‑79) | trpS → / → zapE | tryptophan‑‑tRNA ligase/nucleoside triphosphate hydrolase domain‑containing protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 1,499,506 | 1 | . | C | 88.9% | 44.7 / 1.2 | 18 | intergenic (+83/‑81) | trpS/zapE | tryptophan‑‑tRNA ligase/nucleoside triphosphate hydrolase domain‑containing protein |
| Reads supporting (aligned to +/- strand): ref base . (1/1); new base C (5/11); total (6/12) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.01e-01 | |||||||||||
TGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCCACGGCTTTGCCGGTGT‑T‑‑TCGGGCAC‑G‑CCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTCCCGACTTCTTCCATGA > NC_002947/1499363‑1499642 | tGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGGGCCCCCGAAACAGGCCCCGCGGGGCCTGGCACCGGCTTTGCCGGTGT‑TA‑GCGGGCCc‑g‑ccc > 8:127688/1‑147 (MQ=37) ttCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TC‑GCGGGCACAC‑CCGCTCCCACAGACTGATTGcc < 1:140237/148‑1 (MQ=21) tGAACTCAGCCTTGGCACACCCGATGGACTGAGGCGGCCAATCGGTGGGATGGGGCGTGGCCGCGAAACAGGGGCCGCGGTGCATGCCACCGGCTTTGCCGTGGT‑TC‑TCGGGCAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCgg < 6:67059/148‑1 (MQ=16) gCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TC‑GCGGGCAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACa < 5:84012/147‑1 (MQ=21) cacaGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCCTGCCCGCGAAACACGCGCCCCCGTGCATGGCACCCGCTTTTCCGGTGT‑TAGCCGGCCCC‑C‑CCCCTCCCCCAGACAGATTTGCAATAAGCGGGCCGATCGCTAAACTGAc > 6:33351/1‑148 (MQ=255) aCTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCTAAACAGGCGCCGCGGTGCAGGGCACCGGCTTTGCGGGTGT‑TC‑GCGGGCAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGttt < 6:142493/148‑1 (MQ=21) cTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TC‑GCGGGCAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGtttt < 2:137893/148‑1 (MQ=21) gAGGCTGCCAAACGGGGGGAGCGGGCGTGCCCGCGAAACCGGCGCCGCGGTGCAGGGCACCGGCTTGGCCGGTGTGC‑‑GCGGGCAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGtttttt < 3:149412/148‑1 (MQ=21) ggCTGCCAAACGGGGGGAGCGGGCGGGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCGTTGCCGGTGTGC‑‑GCGGGCAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCCATCGCTACAGTGACGGCCCGTTTTttgt < 7:82742/148‑1 (MQ=21) gCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACCGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TA‑GCGGGCCC‑GCCCGCTGCCACAGACTGGTTGCCATTAAGCGGGGCTATCGCTACAGGGTCGGCCCGGTTTttgtt > 8:86563/1‑148 (MQ=18) gAAACAGGCGCCGCGGTGCATGGCACCGGCTGTGCCGGTGT‑TC‑GCGGGCAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGaa < 2:11529/148‑1 (MQ=37) cAGGCGCCGCGGTGCTTGGCACGGGCTTTGCGGGTGTGG‑‑GGGGGCAG‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCt < 1:103692/148‑1 (MQ=255) cAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TC‑GCGGGCAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGGCCGGTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCt > 3:126301/1‑148 (MQ=37) ccgcGGTGCATGGCACCGGCTTTGCCGGTGT‑TC‑GCGGGCAC‑GCCCGCTCCCACAGCCTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCaa > 7:146959/1‑147 (MQ=37) ggcaccGGCTTTGCCGGTGT‑TC‑GCGGGCAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTGTCAAGCAGATCTGaaa > 1:98290/6‑148 (MQ=38) gtgttcgcGGGCAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGCCCCGACTTCTTcc < 6:46098/141‑1 (MQ=255) gttcgcGGGAAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTCCCGACTTCTTCCAt < 6:118313/143‑1 (MQ=255) gtgcgcGGGCAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTCCCGACTTCTTCCAt < 2:62696/143‑1 (MQ=255) tcgcGGGCAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTCCCGACTTCTTCCATGa > 8:153181/4‑148 (MQ=255) | TGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCCACGGCTTTGCCGGTGT‑T‑‑TCGGGCAC‑G‑CCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTCCCGACTTCTTCCATGA > NC_002947/1499363‑1499642 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |