Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC	NC_002947	1,843,537	Δ3 bp	coding (1255‑1257/2754 nt)	gacS ←	sensor protein GacS

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_002947	= 1843536	1 (0.030)	40 (1.290)	34/288	0.1	97.6%	coding (1258/2754 nt)	gacS	sensor protein GacS
	?	NC_002947	1843540 =	1 (0.030)					coding (1254/2754 nt)	gacS	sensor protein GacS

CAGTGAGTTGTCAGCCTGGCTGAAGGCCTGGAACAGTGTGCGCACATCCTGAGGTGACAGGCCGATGCCGGTGTCCTGCACGCTGATGCGCAACTGCGCGCAGTCTTCGTGCTCGTCTTCCAGCATGGCGCGCACGACGATGGTGCC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_002947/1843390‑1843536 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑cGCGGGTGAACTTGATGGCGTTGCTGACCAGGTTGGTGAGGATCTGCTTGAGCCGCAACGGGTCGCCTACCAGCGAAGCAGGTGTGTCGCGGTAGATCAGGCTCAGCAATTCCAGCTGCTTGGCGTGGGCGGCCGGAGCGAGGATGG  >  NC_002947/1843540‑1843685                                                                                                                                                                                                                                                                                                       CAGTGAGTTGTCAGCCTGGCTGAAGGCCTGGAACAGTGTGCGCACATCCTGAGGTGACAGGCCGATGCCGGTGTCCTGCACGCTGATGCGCAACTGCGCGCAGTCTTCGTGCTCGTCTTCCAGCATGGCGCGCACGACGATGGTGCCG                                                                                                                                                   >  8:83549/1‑148       GTTGTCAGCCTGGCTGAAGGCCTGGAACAGTGTGCGAACATCCTGACGTGACAGGCCCATCCCGGTTTCCTGAACGCTGAAGCCCAACTGCGCGCAGTCTACGTACTCGTCTTCCAGCATCCCGCGCACGACGATGCTGCCGCGcga                                                                                                                                              >  2:105310/1‑144          GTCAGCCTGGCTGAAGGCCTGGAACAGTGTGCGCACATCCTGAGGTGACAGGCCGATGCCGGTGTCCTGCACGCTGATGCGCAACTGCGCGCAGTCTTCGTGCTCGTCTTCCAGCATGGCGCGCACGACGATGGTGCCGCGGGTGAAC                                                                                                                                          >  3:156179/1‑148              GCCTGGCTGAAGGCCTGGAACAGTGTGCGCACATCCTGAGGTGACAGGCCGATGCCGGTGTCCTGCACGCTGATGCGCAACTGCGCGCAGTCTTCGTGCTCGTCTTCCAGCATGGCGCGCACGACGATGGTGCCGCGGGTGAACTTGA                                                                                                                                      >  8:107551/1‑148                          GCCTGGAACAGTGTGCGCACATCCTGAGGTGACAGGCCGATGCCGGTGTCCTGCACGCTGATGCGCAACTGCGCGCAGTCTTCGTGCTCGTCTTCCAGCATGGCGCGCACGACGATGGTGCCGCGGGTGAACTTGATGGCGTTGCTGA                                                                                                                          >  6:5141/1‑148                            CTGGAACAGTGTGCGCACATCCTGAGGTGACAGGCCGATGCCGGTGTCCTGCACGCTGATGCGCAACTGCGCGCAGTCTTCGTGCTCGTCTTCCAGCATGGCGCGCACGACGATGGTGCCGCGGGTGAACTTGATGGCGTTGCTGA                                                                                                                          >  2:106980/1‑146                                      GTGCGCACATCCTGAGGTGACAGGCCGATGCCGGTGTCCTGCACGCTGATGCGCAACTGCGCGCAGTCTTCGTGCTCGTCTTCCAGCATGGCGCGCACGACGATGGTGCCGCGGGTGAACTTGATGGCGTTGCTGACCAGGTTGGTGA                                                                                                              <  5:154960/148‑1                                            ACATCCTGAGGTGACAGGCCGATGCCGGTGTCCTGCACGCTGATGCGCAACTGCGCGCAGTCTTCGTGCTCGTCTTCCAGCATGGCGCGCACGACGATGGTGCCGCGGGTGAACTTGATGGCGTTGCTGACCAGGTTGGTGAGGATCT                                                                                                        >  1:31667/1‑148                                            ACATCCTGAGGTGACAGGCCGATGCCGGTGTCCTGCACGCTGATGCGCAACTGCGCGCAGTCTTCGTGCTCGTCTTCCAGCATGGCGCGCACGACGATGGTGCCGCGGGTGAACTTGATGGCGTTGCTGACCAGGTTGGTGAGGATCT                                                                                                        >  4:69447/1‑148                                               TCCTGAGGTGACAGGCCGATGCCGGTGTCCTGCACGCTGATGCGCAACTGCGCGCAGTCTTCGTGCTCGTCTTCCAGCATGGCGCGCACGACGATGGTGCCGCGGGTGAACTTGATGGCGTTGCTGACCAGGTTGGTGAGGATCTGCT                                                                                                     <  4:140192/148‑1                                                  TGAGGTGACAGGCCGATGCCGGTGTCCTGCACGCTGATGCGCAACTGCGCGCAGTCTTCGTGCTCGTCTTCCAGCATGGCGCGCACGACGATGGTGCCGCGGGTGAACTTGATGGCGTTGCTGACCAGGTTGGTGAGGATCTGCTTGA                                                                                                  <  4:156179/148‑1                                                  TGAGGTGACAGGCCGATGCCGGTGTCCTGCACGCTGATGCGCAACTGCGCGCAGTCTTCGTGCTCGTCTTCCAGCATGGCGCGCACGACGATGGTGCCGCGGGTGAACTTGATGGCGTTGCTGACCAGGTTGGTGAGGATCTGCTTGA                                                                                                  >  2:5503/1‑148                                                           AGGCCGATGCCGGTGTCCTGCACGCTGATGCGCAACTGCGCGCAGTCTTCGTGCTCGTCTTCCAGCATGGCGCGCACGACGATGGTGCCGCGGGTGAACTTGATGGCGTTGCTGACCAGGTTGGTGAGGATCTGCTTGAGCCGCAACG                                                                                         >  1:25190/1‑148                                                            GGCGGATGCCGGTGTCCTGCACGCTGATGCGCAACTGCGCGCAGTCTTCGTGCTCGTCTTCCAGCATGGCGCGCACGACGATGGTGCCGCGGGTGAACTTGATGGCGTTGCTGACCAGGTTGGTGAGGATCTGCTTGAGCCGCAACGG                                                                                        <  3:19320/148‑1                                                               CGATGCCGGTGTCCTGCACGCTGATGCGCAACTGCGCGCAGTCTTCGTGCTCGTCTTCCAGCATGGCGCGCACGACGATGGTGCCGCGGGTGAACTTGATGGCGTTGCTGACCAGGTTGGTGAGGATCTGCTTGAGCCGCAACGGGTC                                                                                     <  2:148072/148‑1                                                                GATGCCGGTGTCCTGCACGCTGATGCGCAACTGCGCGCAGTCTTCGTGCTCGTCTTCCAGCATGGCGCGCACGACGATGGTGCCGCGGGTGAACTTGATGGCGTTGCTGACCAGGTTGGTGAGGATCTGCTTGAGCCGCAACGGGTCG                                                                                    >  8:28861/1‑148                                                                GATGCCGGTGTCCTGCACGCTGATGCGCAACTGCGCGCAGTCTTCGTGCTCGTCTTCCAGCATGGCGCGCACGACGATGGTGCCGCGGGTGAACTTGATGGCGTTGCTGACCAGGTTGGTGAGGATCTGCTTGAGCCGCAACGGGTCG                                                                                    >  4:160159/1‑148                                                                 ATGCCGGTGTCCTGCACGCTGATGCGCAACTGAGCGCAGTCTTCCTGCTCCTCTTCCAGCATGGCGCGCACGAACATGGTGCCGAGGGTCAACTTGATGGCGTTGCTGACAAGGTTGGTGAGGATCTGATTAAGCCGCAACGGGTCGC                                                                                   >  8:43543/1‑148                                                                         GTCCTGCACGCTGATGCGCAACTGCGCGCAGTCTTCGTGCTCGTCTTCCAGCATGGCGCGCACGACGATGGTGCCGCGGGTGAACTTGATGGCGTTGCTGACCAGGTTGGTGAGGATCTGCTTGAGCCGCAACGGGTCGCCTACCAGC                                                                           <  8:160122/148‑1                                                                            CTGCACGCTGATGCGCAACTGCGCGCAGTCTTCGTGCTCGTCTTCCAGCATGGCGCGCACGACGATGGTGCCGCGGGTGAACTTGATGGCGTTGCTGACCAGGTTGGTGAGGATCTGCTTGAGCCGCAACGGGTCGCCTACCAGCGAA                                                                        >  7:29569/1‑148                                                                             TGCACGCTGATGCGCAACTGCGCGCAGTCTTCGTGCTCGTCTTCCAGCATGGCGCGCACGACGATGGTGCCGCGGGTGAACTTGATGGCGTTGCTGACCAGGTTGGTGAGGATCTGCTTGAGCCGCAAGGGGTCGCCTACCAGCGAA                                                                        >  6:105553/1‑147                                                                             TGCACGCTGATGCGCAACTGCGCGCAGTCTTCGTGCTCGTCTTCCAGCATGGCGCGCACGACGATGGTGCCGCGGGTGAACTTGATGGCGTTGCTGACCAGGTTGGTGAGGATCTGCTTGAGCCGCAACGGGTCGCCTACCAGCGAA                                                                        >  3:155423/1‑147                                                                                ACGCTGATGCGCAACTGCGCGCAGTCTTCGTGCTCGTCTTCCAGCATGGCGCGCACGACGATGGTGCCGCGGGTGAACTTGATGGCGTTGCTGACCAGGTTGGTGAGGATCTGCTTGAGCCGCAAGGGGTCGCCTACCAGCGAAGC                                                                      <  5:105553/146‑1                                                                                    TGATGCGCAACTGCGCGCAGTCTTCGTGCTCGTCTTCCAGCATGGCGCGCACGACGATGGTGCCGCGGGTGAACTTGATGGCGTTGCTGACCAGGTTGGTGAGGATCTGCTTGAGCCGCAACGGGTCGCCTACCAGCGAAGCAGGTGT                                                                >  5:165180/1‑148                                                                                    TGATGCGCAACTGCGCGCAGTCTTCGTGCTCGTCTTCCAGCATGGCGCGCACGACGATGGTGCCGCGGGTGAACTTGATGGCGTTGCTGACCAGGTTGGTGAGGATCTGCTTGAGCCGCAACGGGTCGCCTACCAGCGAAGCAGGTGT                                                                >  1:46387/1‑148                                                                                          GCAACTGCGCGCAGTCTTCGTGCTCGTCTTCCAGCATGGCGCGCACGACGATGGTGCCGCGGGTGAACTTGATGGCGTTGCTGACCAGGTTGGTGAGGATCTGCTTGAGCCGCAACGGGTCGCCTACCAGCGAAGCAGGTGTGTCGCG                                                          >  5:158676/1‑148                                                                                          GCAACTGCGCGCAGTCTTCGTGCTCGTCTTCCAGCATGGCGCGCACGACGATGGTGCCGCGGGTGAACTTGATGGCGTTGCTGACCAGGTTGGTGAGGATCTGCTTGAGCCGCAACGGGTCGCCTACCAGCGAAGCAGGTGTGTCGCG                                                          >  3:6793/1‑148                                                                                          GCAACTGCGCGCAGTATTCGTGCTCGTCTTCCAGCATGGCTCGCACGACGATGGTGCCGCGGGTGAACTTGATGGCGTTGCTGACCAGGTTGGTGAGGATCTTCTTGAGCCGCAACGGGTCGCCTACCAGCGAAGCAGGTGTGTCGC                                                           <  6:148300/147‑1                                                                                                          TTCGTGCTCGTCTTCCAGCATGGCGCGCACGACGATGGTGCCGCGGGTGAACTTGATGGCGTTGCTGACCAGGTTGGTGAGGATCTGCTTGAGCCGCAACGGGTCGCCTACCAGCGAAGCAGGTGTGTCGCGGTAGATCAGGCTCAG                                           >  6:76524/1‑147                                                                                                           TCGTGCTCGTCTTCCAGCAGGGCGCGCACGACGATGGTGCCGCGGGTGAACTTGATGGCGTTGCTGACCAGGTTGGTGAGGATCTGCTTGAGCCGCAACGGGTCGCCTACCAGCGAAGCAGGTGTGTCGCGGTAGATCAGGCTCAGC                                          <  8:161947/147‑1                                                                                                              TGCTCGTCTTCCAGCATGGCGCGCACGACGATGGTGCCGCGGGTGAACTTGATGGCGTTGCTGACCAGGTTGGTGAGGATCTGCTTGAGCCGCAACGGGTCGCCTACCAGCGAAGCAGGTGTGTCGCGGTAGATCAGGCTCAGCAATT                                      >  5:133106/1‑148                                                                                                                 TCGTCTTCCAGCATGGCGCGCACGACGATGGTGCCGCGGGTGAACTTGATGGCGTTGCTGACCAGGTTGGTGAGGATCTGCTTGAGCCGCAACGGGTCGCCTACCAGCGAAGCAGGTGTGTCGCGGTAGATCAGGCTCAGCAATTCC                                    <  5:36729/147‑1                                                                                                                      TTCCAGCATGGCGCGCACGACGATGGTGCCGCGGGTGAACTTGATGGCGTTGCTGACCAGGTTGGTGAGGATCTGCTTGAGCCGCAACGGGTCGCCTACCAGCGAAGCAGGTGTGTCGCGGTAGATCAGGCTCAGCAATTCCAGCTGC                              >  4:98006/1‑148                                                                                                                                     CACGACGATGGTGCCGCGGGTGAACTTGATGGCGTTGCTGACCAGGTTGGTGAGGATCTGCTTGAGCCGCAACGGGTCGCCTACCAGCGAAGCAGGTGTGTCGCGGTAGATCAGGCTCAGCAATTCCAGCTGCTTGGCGTGGGCGGCC               <  2:116796/148‑1                                                                                                                                      ACGACGATGGTGCCGCGGGTGAACTTGATGGCGTTGCTGACTAGGTTGGTGAGGATCTGCTTGAGCCGCAACGGGTCGCCTACCAGCGAAGCAGGTGTGTCGCGGTAGATCAGGCTCAGCAATTCCAGCTGCTTGGCGTGGGCGGCC               >  3:158295/1‑147                                                                                                                                        GACGATGGTGCCGCGGGTGAACTTGATGGCGTTGCTGACTAGGTTGGTGAGGATCTGCTTGAGCCGCAACGGGTCGCCTACCAGCGAAGCAGGTGTGTCGCGGTAGATCAGGCTCAGCAATTCCAGCTGCTTGGCGTGGGCGGCCGGA            <  4:158295/148‑1                                                                                                                                        GACGATGGTGCCGCGGGTGAACTTGATGGCGTGGCTGACCAGGTTGGGGAGGATCTGCTTGAGCCGCAACGGGTCGCCTACCAGCGAAGCAGGTGTGTCGCGGTAGATCAGGCTCAGCAATTCCAGCTGCTTGGCGTGGGCGGCCGGA            <  8:114951/148‑1                                                                                                                                            ATGGTGCCGCGGGTGAACTTGATGGCGTTGCTGACCAGGTTGGTGAGGATCTGCTTGAGCCGCAACGGGTCGCCTACCAGCGAAGCAGGTGTGTCGCGGTAGATCAGGCTCAGCAATTCCAGCTGCTTGGCGTGGGCGGCCGTAGCGA        <  3:160159/148‑1                                                                                                                                                TGCCGCGGGTGAACTTGATGGCGTTGCTGACCAGGTTGGTGAGGATCTGCTTGAGCCGCAACGGGTCGCCTACCAGCGAAGCAGGTGTGTCGCGGTAGATCAGGCTCAGCAATTCCAGCTGCTTGGCGTGGGCGGCCGGAGCGAGGAT    <  7:83549/148‑1                                                                                                                                                  CCGCGGGTGAACTTGATGGCGTTGCTGACGAGGTTGGTGAGGATCTGCTTGAGCCGCAACGGGTCGCCTACCAGCGAAGCAGGTGTGTCGCGGTAGATCAGGCTCAGCAATTCCAGATGCTTGGCGTGGGCGGCCGGAGCGAGGATGG  >  5:76697/1‑148                                                                                                                                                                                                                                                                                                       CAGTGAGTTGTCAGCCTGGCTGAAGGCCTGGAACAGTGTGCGCACATCCTGAGGTGACAGGCCGATGCCGGTGTCCTGCACGCTGATGCGCAACTGCGCGCAGTCTTCGTGCTCGTCTTCCAGCATGGCGCGCACGACGATGGTGCC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_002947/1843390‑1843536 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑cGCGGGTGAACTTGATGGCGTTGCTGACCAGGTTGGTGAGGATCTGCTTGAGCCGCAACGGGTCGCCTACCAGCGAAGCAGGTGTGTCGCGGTAGATCAGGCTCAGCAATTCCAGCTGCTTGGCGTGGGCGGCCGGAGCGAGGATGG  >  NC_002947/1843540‑1843685

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.