| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 5,988,848 | 0 | C | T | 43.5% | 17.6 / 22.8 | 23 | intergenic (+142/‑404) | kefB‑III/PP_mr64 | glutathione‑regulated potassium/H+ antiporter/C4 ncRNA |
| Reads supporting (aligned to +/- strand): ref base C (10/3); new base T (6/4); total (16/7) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.50e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
AGGCCTTTTGGCGCGTGCCTTGAGGGTTGTAGTGCCTGTGAGATTGGGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGTTTGTTTCGGGCCAGTTATTCCTGTGGGACGCGCACGCGAGCCCCCTGGCGCACGCCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGCCTGGCACAGGAGGGATTGGCCAGAAACAAACGTAGGAGCGGGTTTACCCGCGAAGCGCCGCGCGGGCGGCGCTCGATCTCATGGGCGCCCAAAAACTATC > NC_002947/5988706‑5988989 | aGGCCTTTTGGCGCGTGCCTTGAGGGTTGTAGTGCCTGTGAGATTGGGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGTTTGTTTCGGGCCAGTTATTCCTGTGGGACGCGCACGCGAGCCCCCTGGCGCACGCCt > 2:8363/1‑147 (MQ=255) cTTGAGGGTTGTAGTGCCTGTGAGATTGGGCGCCGCCCGCGCGGCGCGTCGCGGGTAAACCCGCTCCTACGTTTGTTTCGGGCCAGTTATTCCTGTGGGACGCGCACGCGAGCCCCCTGGCGCACGCCTCGATATCGCGTCGTacaaa < 6:16756/148‑1 (MQ=255) ccgcccgcGCGGCGCTTCGCGGGTAAACCCGCTCCTACGTTTGTTTCGGGCCAATTATTCCTGCGGGATTTGCGCGCGACCGCCTTGGGGCATGTCTCGATATCGCGTCGTacaaa > 6:162136/1‑116 (MQ=14) cgcccgcGCGGCGCTTCGCGGGTAAACCCGCTCCTACGTTTGTTTCGGGCCAATTATTCCTGCGGGATTTGCGCGCGACCGCCTTGGGGCATGTCTCGATATCGCGTCGTacaaac < 5:162136/116‑1 (MQ=14) gcgcgGCGCTTCGCGGGTAAAACCCCAACAACCTTTTTTTAGGGCCAGGTATTACGGTGGGACGCCCCCCCCCGCCCCCAGGCGCACGCCACCAAAACGCGTCGTACAAACAACGCCGGCGCGCGCGCCCGGCCCAGGAGGGATTgg > 8:73156/1‑147 (MQ=255) ggTAAACCCGCTCCTACGTTTGTTTCGGGCCAGTTATTCCTGTGGGACGCGCCCGCGAGCCCCCTGGCGCACGCCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGCCTGGCACAGGAGGGATTGGCCAGAAACAAACGTa > 3:42330/1‑147 (MQ=255) aaCCCGCTCCTACGTTTGTTTCGGGCCAATTATTCCTGCGGGATTTGCGCGCGACCGCCTTGGGGCATGTCTCGATATCGCGTGGTACCAACACGGCGGTCGCGCGCGCCTGGTGGAGGAGGGACTGGCC‑GAAACCAACGTAGGAGCg > 7:49306/1‑148 (MQ=16) cccGCTCCTACGTTTGTTTCGGGCCAGTTATTCCTGTGGGACGCGCACGCGAGCCCCCTGGCGCACGCCTCGATATCGCGTCGTACAAACAAGGCGGGCGCGCGCGCCTGGCACAGGAGGGATTGGCCAGAAACAAACGTAGGAGCgg < 1:8363/148‑1 (MQ=255) tCCTACGTTTGTTTCGGGCCAGTTATTCCTGTGGGACGCGCACGCGAGCCCCCTGGCGCACGCCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGCCTGGCACAGGAGGGATTGGCCAGAAACAAACGTAGGAGCGGGTTTa > 7:155130/1‑148 (MQ=255) tCCTACGTTTGTTTCGGGCCAATTATTCCTGCGGGATTTGCGCTCGACCGCCTTGGGGCATGGCTCGATATCGCGTCGTACTAACAAGGCGGTCGCGCGCGACTGGTGCAGGAGGGACTGGCC‑GAAACAAACGTAGGAGCGGGTTTAc < 8:134915/148‑1 (MQ=255) ccTACGTTTGTTTCGGGCCAATTATTCCTGCGGGATTTGCGCGCGACCGCCTTGGGGCATGTCTCGATATCGCGTCGTACAAACACGGCGGTCGCGCGCGACTGGTGCAGGAGGGACTGGCC‑GAAACAACCGTAGGCGCGGGTTTAcc > 5:47347/1‑148 (MQ=255) tACGTTTGTTTCGGGCCAATTATTCCTGCGGGATTTGCGCGCGACCGCCTTGGGGCATGTCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGACTGGTGCAGGAGGGACTGGCC‑GAAACAAACGTAGGAGCGGGTTTACCcg > 6:154602/1‑148 (MQ=11) gtttgtttCGGGCCAGTTATTCCTGTGGGACGCGCACGCGAGCCCCCTGGCGCACGCGTCGATATCGCGGCGTACAAACAAGGCGGTCGCGCGCGCCTGGCCCGGGAGGGATTGGCCAGAAACAAACGTAGGAGCGGGTTTACCcgc > 7:118854/1‑147 (MQ=255) ttgttgCGGGCCAGTTCTGCCTGTGGGACGCGCACGCGAGCCCCCGGGCGCACGCCTCGATATCGCGGCGTACAAACAAGGCGGTCGCGCGCGCCGGGCACAGGAGGGATTGGCCAGAAACAAACGTAGGAGCGGGTTTACCCGCGaa < 7:73156/148‑1 (MQ=25) ccAGTTATTCCTGTGGGACGCGCACGCGAGCCCCCTGGCGCACGCCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGCCTGGCACAGGAGGGATTGGCCAGAAACAAACGTAGGAGCGGGTTTACCCGCGAAGCGCcgcgcg > 2:121788/1‑148 (MQ=255) ttATTCCTGTGGGACGCGCACGCGAGCCCCCTGGCGCACGCCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGCCTGGCACAGGAGGGATTGGCCAGAAACAAACGTAGGAGCGGGTTTACCCGCGAAGCGCCGCgcgggcg > 4:121741/1‑148 (MQ=255) gcgACCGCCTTGGGGCATGTCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGACTGGTGCAGGAGGGACTGGCC‑GAAACAAACGTAGGAGCGGGTTTACCCGCGACGCGCCGCGCGGGCGGCGCTCGATCTCACAGgcgct > 1:46520/1‑146 (MQ=14) accgccttGGGGCATGTCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGACTGGTGCAGGAGGGACTGGCC‑GAAACAAACGTAGGAGCGGGTTTACCCGCGAAGCGCCGCGCGGGCGGCGCTCGATCTCACAGgcg < 6:7271/135‑1 (MQ=14) cgccttGGGGCATGTCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGACTGGTGCAGGAGGGACTGGCC‑GAAACAAACGTAGGAGCGGGTTTACCCGCGAAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGCTGaaaat > 1:164776/6‑147 (MQ=14) cccTGGCGAACGCCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGCCTGGCACAGGAGGGATTGGCCAGAAACAAACGTAGGAGCGGGTTTACCCGCGAAGCGCCGCGCGGGCGGCGCTCGATCTCATGGGCGCCCtaaaa > 1:183481/1‑147 (MQ=255) ccTGGCGCACGCCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGCCTGGCACAGGAGGGATTGGCCAGAAACAAACGTAGGAGCGGGTTTACCCGCGAAGCGCCGCGCGGGCGGCGCTCGATCTCATGGGCGCCCAAAAACt > 1:22290/1‑148 (MQ=255) ttGGGGCATGTCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGACTGGTGCAGGAGGGACTGGCC‑GAAACAAACGTAGGAGCGGGTTTACCCGCGAAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGCTGaaaatcctc < 2:164776/147‑6 (MQ=14) ggCGCACGCCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGCCTGGCACAGGAGGGATTGGCCAGAAACAAACGTAGGAGCGGGTTTACCCGCGAAGCGCCGCGCGGGCGGCGCTCGATCTCATGGGCGCCCAAAAACTATc > 6:6131/1‑148 (MQ=255) | AGGCCTTTTGGCGCGTGCCTTGAGGGTTGTAGTGCCTGTGAGATTGGGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGTTTGTTTCGGGCCAGTTATTCCTGTGGGACGCGCACGCGAGCCCCCTGGCGCACGCCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGCCTGGCACAGGAGGGATTGGCCAGAAACAAACGTAGGAGCGGGTTTACCCGCGAAGCGCCGCGCGGGCGGCGCTCGATCTCATGGGCGCCCAAAAACTATC > NC_002947/5988706‑5988989 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |