| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_002947 | 2,921,460 | G→A | G3494R (GGG→AGG) | PP_2561 → | hemolysin‑type calcium‑binding bacteriocin |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 2,921,460 | 0 | G | A | 94.4% | 51.9 / NA | 18 | G3494R (GGG→AGG) | PP_2561 | hemolysin‑type calcium‑binding bacteriocin |
| Reads supporting (aligned to +/- strand): ref base G (0/0); major base A (12/5); minor base T (0/1); total (12/6) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.33e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GCCGGTAACGACAACCTGAACGGGGGCGCTGGCGATGACAGCCTGGATGGCGGCAACGGCAATGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCGGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGGTGGTGGGTGGGGCCGGTAACGACACCATGATCGCCACCGATGGAAACGATGTGTTCCAGTTCGCTGCAGGCTTTGGCAACGACCTGATCATCA > NC_002947/2921331‑2921601 | gCCGGTAACGACAACCTGCCCGGGGTCGCTGGCGATGACAGCCTGGAGGGCGGCAACGGCAATGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCCGGCAACGACt < 1:88784/148‑1 (MQ=255) cTGAACGGGGGCGCTGGCGATGACAGCCTGGATGGCGGCAACGGCAATGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCAACACCCTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCg > 3:171426/1‑147 (MQ=255) tGAACGGGGGCGCTGGCGATGACAGCCTGGATGGCGGCAACGGCAATGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCg > 3:63693/1‑146 (MQ=255) gAACGGGGGCGCTGGCGATGACAGCCTGGATGGCGGCAACGGCAATGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCa > 2:107481/1‑148 (MQ=255) gcTGGCGATGACATCCTGGATGGCGGCAACGGCAATGACACCCTTCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCGTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATa < 3:94746/148‑1 (MQ=255) tGGCGATGACAGCCTGGATGGCGGCAACGGCAATGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACg > 3:43933/1‑148 (MQ=255) tGGCGATGACAGCCTGGATGGCGGAAACGGCAATGACACCCTGCAGGTCGGCGATGGTAACGACACATTGTTCGGCGCCGTGGGTACCGACACCCTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACg > 7:139221/1‑148 (MQ=255) tGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGGTGGTGGGTGGGGCCGGTAACGACACCATGATCGCCACCGa > 7:71419/1‑148 (MQ=255) cccgggCGGCGAGCGTAACGACACATTGGTCGGCGTCGTGGGTCCCGACCCCCTGCGCTGGGGGGCCGGCAACGACTTCCTCAACGGCGCGGCAGGTAACGATACGGTGGTGGGGGGGGCCGGTAACGACACCATGATCGCCACCGAt < 2:60987/145‑1 (MQ=255) aaCGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGGTGGTGGGTGGGGCCGGTAACGACACCATGATCGCCACCGATGGAAACGa > 7:161388/1‑140 (MQ=255) aCGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGGTGGTGGGTGGGGCCGGTAACGACACCATGATCGCCACCGATGGAAACGAt < 8:161388/140‑1 (MQ=255) gACACATTGTTCGGCGACGTGGGTACCGACCCCCTGATCAGGGGGGCCGGCAACGCCTTCCTCAACGGT‑‑GGCAGGTAACGATACGGTGGTGGGTGGGGCCGGTAACGACACCATGATCGCCACCTATGGAAACGCTGTGTTCCAGTTc > 5:128354/1‑148 (MQ=255) acaTTGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGGTGGTGGGTGGGGCCGGTAACGACACCATGATCGCCACCGATGGAAACGATGTGTTCCAGTTCg > 2:33257/1‑148 (MQ=255) ttCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGGTGGTGGGTGGGGCCGGTAACGACACCATGATCGCCACCGATGGAAACGATGTGTTCCAGTTCGCTGCAg > 2:177559/1‑148 (MQ=255) gCGACGTGGGTACCGACCCCGTGATCAGGGGGGCCGGCAACGACTTCCTCTTCGGTGCGGCAGTTAACGATACGGTGGTGGGTGGGGCCGGTAACGACACCATGATCGCCACCGATGGAAACGATGTGTTCCAGTTCGCTGCAGGCtt < 1:35058/148‑1 (MQ=255) ttcagACACCCTTCTCAGCGGGTCCGTCATCGACATCCTCTAGGGGGGTGCAGGTAACGATACGTTGGTGGGGGGGGCCGGTAACGACACCATGATCGCCACCGATGGAAACGATGTGTTCCAGTTCGCTGCAGGCTTTGGCAACGAc < 4:163275/144‑1 (MQ=255) ccTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGGTGGTGGGTGGGGCCGGTAACGACACCATGATCGCCACCGATGGAAACGATGTGTTCCAGTTCGCTGCAGGCTTTGGCAACGACCTGatcat > 3:72488/1‑147 (MQ=255) cTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGGTGGTGGGTGGGGCCGGTAACGACACCATGATCGCCACCGATGGAAACGATGTGTTCCAGTTCGCTGCAGGCTTTGGCAACGACCTGAtcatca > 3:143861/1‑148 (MQ=255) | GCCGGTAACGACAACCTGAACGGGGGCGCTGGCGATGACAGCCTGGATGGCGGCAACGGCAATGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCGGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGGTGGTGGGTGGGGCCGGTAACGACACCATGATCGCCACCGATGGAAACGATGTGTTCCAGTTCGCTGCAGGCTTTGGCAACGACCTGATCATCA > NC_002947/2921331‑2921601 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |