Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_002947	3,788,322	G→A	Q105* (CAG→TAG)	PP_3350 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	3,788,322	0	G	A	100.0%	112.3 / NA	34	Q105* (CAG→TAG)	PP_3350	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (15/19);  total (15/19)

TGAAATCGACGAAGGCCTGTCGCACTTCATTGCGGCTTTCATCATTGGGCGAGTACAAGTCCTTGCCAAAGGCACGGGTATTCTCGACCTGGACGAAGGTACGCACGGCATCGTCGAACAGGTGCAGGTCGGCATGGGCCTGCAGCCGTTGCTGCAGATAGGAATCGTCGTTCAGCCCGCGCAGGCCAAAGAAGGGTTTGTCCGAAGCATCGGCCCGGTAGCGGGCCTCAGCCCCGAGTTGCAGCCAGGCCGACTCTGACAGCCGATGGTAGCGCAGGCCATCG  >  NC_002947/3788181‑3788464                                                                                                                                              |                                                                                                                                               tGAAATCGACGAAGGCCTGTCGCACTTCATTGCGGCTTTCATCATTGGGCGAGTACAAGTCCTTGCCAAAGGCACGGGTATTCTCGACCTGGACGAAGGTACGCACGGCATCGTCGAACAGGTGCAGGTCGGCATGGGCCTACAGCCg                                                                                                                                          <  2:177363/148‑1 (MQ=255)         acgaAGGCCTGTCGCACTTCGTTGCGGCTTTCATCATTGGGCGAGTACAAGTCCTTGCCAAAGGCACGGGTATTCTCGACCTGGACGAAGGTACGCACGGCATCGTCGAACAGGTGCAGGTCGGCATGGGCCTACAGCCGTtgctgc                                                                                                                                   <  5:13226/147‑1 (MQ=255)           gaAGGCCTGTCGCACTTCATTGCGGCTTTCATCATTGGGCGAGTACAAGTCCTTGCCAAAGGCACGGGTATTCTCGACCTGGACGAAGGTACGCACGGCATCGTCGAACAGGTGCAGGTCGGCATGGGCCTACAGCCGTTGCTGCAGa                                                                                                                                <  7:75107/148‑1 (MQ=255)                             aTTGCGGCTTTCATCATTGGGCGAGTACAAGTCCTTGCCAAAGGCACGGGTATTCTCGACCTGGACGAAGGTACGCACGGCATCGTCGAACAGGTGCAGGTCGGCATGGGCCTACAGCCTTTGCTGCAGATAGGAATCGTCGTTCAg                                                                                                               <  4:119368/147‑1 (MQ=255)                              ttGCGGCTTTCATCATTGGGCGAGTACAAGTCCTTGCCAAAGGCACGGGTATTCTCGACCTGGACGAAGGTACGCACGGCATCGTCGAACAGGTGCAGGTCGGCATGGGCCTACAGCCGTTGCTGCAGATAGGAATCGTCGTTCAGc                                                                                                              <  8:65086/147‑1 (MQ=255)                                    cTTTCATCATTGGGCGAGTACAATTCCTTGCCAAAGGCACGGGTATTCTCGACCTGGACGAAGGTACGCACGGCTTGGTCGAACAGGTGCAGGTGGGCATGGGCCTACAGCCGTTGCTGCAGATAGGAATCGTCGTTCAGCCCGCGCa                                                                                                       <  7:47067/148‑1 (MQ=255)                                    cTTTCATCATTGGGCGAGTACAAGTCCTTGCCAAAGGCACGGGTATTCTCGACCTGGACGAAGGTACGCACGGCATCGTCGAACAGGTGCAGGTCGGCATGGGCCTACAGCCGTTGCTGCAGATAGGAATCGTCGTTCAGCCCGCGCa                                                                                                       <  7:49335/148‑1 (MQ=255)                                     tttCATCATTGGGCGAGTACAAGTCCTTGCCAAAGGCACGGGTATTCTCGACCTGGACGAAGGTACGCACGGCATCGTCGAACAGGTGCAGGTCGGCATGGGCCTACAGCCGTTGCTGCAGATAGGAATCGTCGTTCAGCCCGCGCAg                                                                                                      >  1:35783/1‑148 (MQ=255)                                                 gCGAGTACAAGTCCTTGCCAAAGGCACGGGTATTCTCGACCTGGACGAAGGTACGCACGGCATCGTCGAACAGGTGCAGGTCGGCATGGGCCTACAGCCGTTGCTGCAGATAGGAATCGTCGTTCAGCCCGCGCAGGCCAAAGAAggg                                                                                          <  2:94722/148‑1 (MQ=255)                                                 gCGAGTACAAGTCCTTGCCAAAGGCACGGGTATTCTAGACCTGGACGAAGGTACGCACGGCATCGTCGAACAGGTGCAGGTCGGCATGGGCCTACAGCCGTTGCTGCAGATAGGAATCGTCGTTCAGCCCGCGCAGGCCAAAGAAggg                                                                                          >  6:1875/1‑148 (MQ=255)                                                  cGAGTACAAGTCCTTGCCAAAGGCACGGGTATTCTAGACCTGGACGAAGGTACGCACGGCATCGTCGAACAGGTGCAGGTCGGCATGGGCCTACAGCCGTTGCTGCAGATAGGAATCGTCGTTCAGCCCGCGCAGGCCAAAGAAGGGt                                                                                         <  5:1875/148‑1 (MQ=255)                                                         aaGTCCTTGCCAAAGGCACGGGTATTCTCGACCTGGACGAAGGTACGCACGGCATCGTCGAACAGGTGCAGGTCGGCATGGGCCTACAGCCGTTGCTGCAGATAGGAATCGTCGTTCAGCCCGCGCAGGCCAAAGAAGGGTTTGTcc                                                                                   >  5:116466/1‑147 (MQ=255)                                                         aaGTCCTTGCCAAAGGCACGGGTATTCTCGACCTGGACGAAGGTACGCACGGCATCGTCGAACAGGTGCAGGTCGGCATGGGCCTACAGCCGTTGCTGCAGATAGGAATCGTCGTTCAGCCCGCGCAGGCCAAAGAAGGGTTTGTcc                                                                                   >  4:85831/1‑147 (MQ=255)                                                            tCCTTGCCAAAGGCACGGGTATTCTCGACCTGGACGAAGGTACGCACGGCATCGTCGAACAGGTGCAGGTCGGCATGGGCCTACAGCCGTTGCTGCAGATAGGAATCGTCGTTCAGCCCGCGCAGGCCAAAGAAGGGTTTGTCCGaa                                                                                >  3:61337/1‑147 (MQ=255)                                                              cTTGCCAAAGGCACGGGTATTCTCGACCTGGACGAAGGTACGCACGGCATCGTCGAACAGGTGCAGGTCGGCATGGGCCTACAGCCGTTGCTGCAGATAGGAATCGTCGTTCAGCCCGCGCAGGCCAAAGAAGGGTTTGTCCGAAGCa                                                                             >  8:130210/1‑148 (MQ=255)                                                               ttGCCAAAGGCACGGGTATTCTCGACCTGGACGAAGGTACGCACGGCATCGTCGAACAGGTGCAGGTCGGCATGGGCCTACAGCCGTTGCTGCAGATAGGAATCGTCGTTCAGCCCGCGCAGGCCAAAGAAGGGTTTGTCCGAAGCAt                                                                            >  5:5849/1‑148 (MQ=255)                                                                  ccAAAGGCACGGGTATTCTCGACCTGGACGAAGGTACGCACGGCATCGTCGAACAGGTGCAGGTCGTCATGGGCCTACAGCCGGTGCTGCAGATAGGAATCGTCGTTCAGCCCGCGCAGGCCAAAGAAGGGTTTGTCCGAAGCATCg                                                                          <  7:32325/147‑1 (MQ=255)                                                                   cAAAGGCACGGGTATTCTCGACCTGGACGAAGGTACGCACGGCATCGTCGAACAGGTGCAGGTCGGCATGGGCCTACAGCCGTTGCTGCAGATAGGAATCGTCGTTCAGCCCGCGCAGGCCAAAGAAGGGTTTGTCCGAAGCATCGGc                                                                        >  1:77044/1‑148 (MQ=255)                                                                            gggTATTCTCGACCTGGACGAAGGTACGCACGGCATCGTCGAACAGGTGCAGGTCGGCATGGGCCTACAGCCGTTGCTGCAGATAGGAATCGTCGTTCAGCCCGCGCAGGCCAAAGAAGGGTTTGTCCGAAGCATCGGCCCGGTAGCg                                                               >  2:170609/1‑148 (MQ=255)                                                                                  tctcGACCTGGACGAAGGTACGCACGGCATCGTCGAACAGGTGCAGGGCGGCATGGGCCTACAGCCGTTGCTGCAGATAGGAATCGTCGTTCAGCCCGCGCAGGCCAAAGAAGGGTTTGTCCGAAGCATCGGCCCGCTACCGGGccga                                                         <  7:115974/148‑3 (MQ=255)                                                                                    tcGACCTGGACGAAGGTACGCACGGCATCGTCGAACAGGTGCAGGTCGGCATGGGCCTACAGCCGTTGCTGCAGATAGGAATCGTCGTTCAGCCCGCGCAGGCCAAAGAAGGGTTTGTCCGAAGCATCGGCCCGGTAGCGGGCCTCa                                                        <  2:104230/147‑1 (MQ=255)                                                                                    tcGACCTGGACGAAGGTACGCACGGCATCGTCGAACAGGTGCAGGTCGGCATGGGCCTACAGCCGTTGCTGCAGATAGGAATCGTCGTTCAGCCCGCGCAGGCCAAAGAAGGGTTTGTCCGAAGCATCGGCCCGGTAGCGGGCCTCAg                                                       >  1:119234/1‑148 (MQ=255)                                                                                    tcGACCTGGACGAAGGTACGCACGGCATCGTCGAACAGGTGCAGGTCGGCATGGGCCTACAGCCGTTGCTGCAGATAGGAATCGTCGTTCAGCCCGCGCAGGCCAAAGAAGGGTTTGTCCGAAGCATCGGCCCGGTAGCGGGCCTCAg                                                       >  2:184575/1‑148 (MQ=255)                                                                                    tcGACCTGGACGAAGGTACGCACGGCATCGTCGAACAGGTGCAGGTCGGCATGGGCCTACAGCCGTTGCTGCAGATAGGAATCGTCGTTCAGCCCGCGCAGGCCAAAGAAGGGTTTGTCCGAAGCATCGGCCCGGTAGCGGGCCTCAg                                                       >  1:44532/1‑148 (MQ=255)                                                                                       aCCTGGACGAAGGTACGCACGGCATCGTCGAACAGGTGCAGGTCGGCATGGGCCTACAGCCGTTGCTGCAGATAGGAATCGTCGTTCAGCCCGCGCAGGCCAAAGAAGGGTTTGTCCGAAGCATCGGCCCGCTACCGGGccgatg                                                       <  6:72162/145‑5 (MQ=255)                                                                                             aCGAAGGTACGCACGGCATCGTCGAACAGGTGCAGGTCGGCATGGGCCTACAGCCGTTGCTGCAGATAGGAATCGTCGTTCAGCCCGCGCAGGCCAAAGAAGGGTTTGTCCGAAGCATCGGCCCGGTAGCGGGCCTCAGCCCCGAGtt                                              <  8:102123/148‑1 (MQ=255)                                                                                             aCGAAGGTACGCACGGCATCGGCGAACAGGTGCAGGTCGGCATGGGCCTACAGCCGTTGCTGCAGATAGGAATCGTCGTTCAGCCCGCGCAGGCCAAAGAAGGGTTTGTCCGAAGCAGCGGCCCGGTAGCGGGCCTCAGCCCCGAGt                                               <  8:4323/147‑1 (MQ=255)                                                                                                  ggTACGCACGGCATCGTCGAAGAGGTGCAGGTCGGCATGGGCCTACAGCCGTTGCTGCAGATAGGAATCGTCGTTCAGCCCGCGCAGGCCAAAGAAGGGTTTGTCCGAAGCATCGGCCCGGTAGCGGGCCTCAGCCCCGAGTTGCAGc                                         <  1:69637/148‑1 (MQ=255)                                                                                                       gCACGGCATCGTCGAACAGGTGCAGGTCGGCATGGGCCTACAGCCGTTGCTGCAGATAGGAATCGTCGTTCAGCCCGCGCAGGCCAAAGAAGGGTTTGTCCGAAGCATCGGCCCGGTAGCGGGCCTCAGCCCCGAGTTGCAGCCAGGc                                    >  5:6293/1‑148 (MQ=255)                                                                                                       gCACGGCATCGTCGAACAGGTGCAGGTCGGCATGGGCCTACAGCCGTTGCTGCAGATAGGAATCGTCGTTCAGCCCGCGCAGGCCAAAGAAGGGTTTGTCCGAAGCATCGGCCCGGTAGCGGGCCTCAGCCCCGAGTTGCAGCCAGGc                                    >  2:34066/1‑148 (MQ=255)                                                                                                        cACGGCATCGTCGAACAGGTGCAGGTCGGCATGGGCCTACAGCCGTTGCTGCAGATAGGAATCGTCGTTCAGCCCGCGCAGGCCAAAGAAGGGTTTGTCCGAAGCATCGGCCCGGTAGCGGGCCTCAGCCCCGAGTTGCAGCCAGGcc                                   <  5:15930/148‑1 (MQ=255)                                                                                                                cgtcgAACAGGTGCAGGTCGGCATGGGCCTACAGCCGTTGCTGCAGATAGGAATCGTCGTTCAGCCCGCGCAGGCCAAAGAAGGGTTTGTCCGAAGCATCGGCCCGGTAGCGGGCCTCAGCCCCGAGTTGCAGCCAGGCCGACTCTGa                           <  1:19693/148‑1 (MQ=255)                                                                                                                            gCAGGTCGGCATGGGCCTACAGCCGTTGCTGCAGATAGGAATCGTCGTTCAGCCCGCGCAGGCCAAAGAAGGGTTTGTCCGAAGCATCGGCCCGGTAGCGGGCCTCAGCCCCGAGTTGCAGCCAGGCCGACTCTGACAGCCGATGGTa               <  6:116466/148‑1 (MQ=255)                                                                                                                                          gCCTACAGCCGTTGCTGCAGATAGGAATCGTCGTTCAGCCCGCGCAGGCCAAAGAAGGGTTTGTCCGAAGCATCGGCCCGGTAGCGGGCCTCAGCCCCGAGTTGCCGCCAGGCCGACTCTGACAGCCGATGGTAGCGCAGGCCATCg  >  8:5986/1‑147 (MQ=255)                                                                                                                                              |                                                                                                                                               TGAAATCGACGAAGGCCTGTCGCACTTCATTGCGGCTTTCATCATTGGGCGAGTACAAGTCCTTGCCAAAGGCACGGGTATTCTCGACCTGGACGAAGGTACGCACGGCATCGTCGAACAGGTGCAGGTCGGCATGGGCCTGCAGCCGTTGCTGCAGATAGGAATCGTCGTTCAGCCCGCGCAGGCCAAAGAAGGGTTTGTCCGAAGCATCGGCCCGGTAGCGGGCCTCAGCCCCGAGTTGCAGCCAGGCCGACTCTGACAGCCGATGGTAGCGCAGGCCATCG  >  NC_002947/3788181‑3788464

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑