Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_002947	4,740,816	T→G	intergenic (+53/+700)	gltA → / ← yeiW	citrate synthase/putative oxidoreductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	4,740,816	0	T	G	100.0%	71.4 / NA	23	intergenic (+53/+700)	gltA/yeiW	citrate synthase/putative oxidoreductase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (12/11);  total (12/11)

AGCCCGTACAAGATCGGCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCCTTTTTTTATTTTTCTGGTGCCGGCCTCTTCGCGGCCTT‑GCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGCGCATCCACCACGAACTTGTTGGCCA  >  NC_002947/4740680‑4740951                                                                                                                                         |                                                                                                                                         aGCCCGTACAAGATCGGCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCg                                                                                                                                <  2:28100/146‑1 (MQ=255)      gTACAAGATCGGCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCtt                                                                                                                         >  7:96342/1‑148 (MQ=255)          aaGATCGGCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTcgcg                                                                                                                     >  7:33352/1‑148 (MQ=255)                        gcgcCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTT‑GCCCGCGaa                                                                                                      <  1:90736/148‑1 (MQ=255)                         cgcCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGGCAA‑GGCCGCGAag                                                                                                     <  4:780/148‑1 (MQ=255)                          gcCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGACAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCGCTTCGCGGGCAA‑GGCCGCGAaga                                                                                                    <  7:80693/148‑1 (MQ=255)                                                   gcgcGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCCGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCGT‑GCCCGCGAAGAGGCCGGCACAGCCACCACAACAccg                                                                           >  5:99389/1‑147 (MQ=255)                                                      cgACATCGGTGCCCGGAAACCCCGCAAGTAAGCTACAGTCGCCGAACGCAAGACGGCTGCCCGCGGGCAGCC‑TTTTTTATTTGCCCTGGGCCGGCCGCTTCGCGGCCTT‑GCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAg                                                                        <  3:139876/148‑1 (MQ=255)                                                       gACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTT‑GCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGt                                                                       >  8:5907/1‑148 (MQ=255)                                                               tGCCCTGAAAGACGGCATTTAAGCTGAATTAGCCTATCGCATTATGGCTGCCGTCGGTCAGCC‑TTTTTTATTTGGCGTGTGCCGGCGTCTTCG‑GGCCTTGGCCCGCTAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTcc                                                               <  4:103686/148‑1 (MQ=255)                                                                  ccTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTT‑GCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTccgcc                                                            >  6:61834/1‑148 (MQ=255)                                                                   cTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTT‑GCCCGCGAAGAGGCCGGCACAGCCACCACAAAACCGAAGTTTTTATccgccc                                                           >  6:51988/1‑148 (MQ=255)                                                                           ccGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTACCGGCCTCTTCGCGGCCTT‑GCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCAc                                                     >  4:107837/1‑146 (MQ=255)                                                                              cAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTT‑GCCCGCGAAGAGGCCGGCACAGACACAACAAAACCTCAGGTTTTATACGCCCGCTCACGCAGc                                                >  8:115298/1‑148 (MQ=255)                                                                                      cTGCAGTCGCCGAACGCACGACGGCGGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGGGCCGGCCGCTGCGCGGCCTG‑GCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAg                                        <  3:165913/148‑1 (MQ=255)                                                                                       tGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTT‑GCCCGCGCAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCCCGCAGCCCTGTCAgg                                       >  5:70376/1‑148 (MQ=255)                                                                                                     gCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCCGCCTT‑GCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGgcg                          >  2:25280/1‑147 (MQ=255)                                                                                                      catgaagGCTGCCCTCGGGCAG‑CATTTTTTATTTGTCCTGTCGCGGCGTATTCGGGGCCTG‑GCCCGCGATGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGCGCa                        <  7:115298/145‑1 (MQ=255)                                                                                                             gcggCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTG‑GCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGCGCATccacca                 <  3:126067/145‑1 (MQ=255)                                                                                                                       ggCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCGTT‑GCCCGCTAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGCGCATCCACCACGAACttgtt       <  3:163751/148‑1 (MQ=255)                                                                                                                        gCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTT‑GCCCGCTAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGCGCATCCACCACGAACttgttg      <  6:33891/148‑1 (MQ=255)                                                                                                                        gCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTT‑GCCCGCGAAGAGGCCGGCCCAGACACCCCAAAACCTCAGGTTTTCTACGCCCGCTCACGCACCCCGTTCAGGGTTTTGAACGGCGCATCCACCCCGAACgtgttg      >  3:115833/1‑148 (MQ=255)                                                                                                                             ccttttttATTTGTCCTGTGCCGGCCTCTTCGCGGCCTT‑GCCCGCGAAGAGGCCGGCACAGACACCACAAACCCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGCGCATCCACCACGAACTTGTTGGccc  >  8:117469/3‑147 (MQ=255)                                                                                                                                         |                                                                                                                                         AGCCCGTACAAGATCGGCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCCTTTTTTTATTTTTCTGGTGCCGGCCTCTTCGCGGCCTT‑GCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGCGCATCCACCACGAACTTGTTGGCCA  >  NC_002947/4740680‑4740951

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑