Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_002947	5,948,274	G→A	S67L (TCG→TTG)	rho ←	transcription termination factor Rho

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,948,274	0	G	A	100.0%	90.6 / NA	27	S67L (TCG→TTG)	rho	transcription termination factor Rho
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (13/14);  total (13/14)

GCAGGGCGAAGTAACGCTCCCCTTCCTTCGGTGGGCGGATCTTGCCGACGATGGTGTCGCCGGTACGCAGGTTGAAACGGCGGATCTGGCTGGGCGAGACATAGATATCGTCTGGGCCGGCCAGGTAGGACGCATCAGCCGAACGCAGGAAACCGAAACCATCCTGGAGAATCTCCAGCACGCCGTCACCCGAGATCTCTTCGCCGCTTTTCGCATGCTTCTTCAGCAGGGCGAAAATCACGTCCTGTTTGCGCGAACGGGCCATGTTTTCGATGCCCATC  >  NC_002947/5948134‑5948414                                                                                                                                             |                                                                                                                                             gcagGGCGAAGTAACGCTCCCCTTCCTTCGGTGGGCGGATCTTGCCGACGATGGTGTCGCCGGTACGCAGGTTGAAACGGCGGATCTGGCTGGGCGAGACATAGATATCGTCTGGGCCGGCCAGGTAGGACGCATCAGCCAAACGCa                                                                                                                                        <  1:23739/147‑1 (MQ=255)           gTAACGCTCCCCTTCCTTCGGTGGGCGGATCTTGCCGACGATGGTGTCGCCGGTACGCAGGTTGAAACGGCGGATCTGGCTGGGCGAGACATAGATATCGTCTGGGCCGGCCAGGTAGGACGCATCAGCCAAACGCAGgaaaccgaaa                                                                                                                             <  1:32605/148‑1 (MQ=255)              aCGCTCCCCTTCCTTCGGTGGGCGGATCTTGCCGACGATGGTGTCGCCGGTACGCAGGTTGAAACGGCGGATCTGGCTGGGCGAGACATAGATATCGTCTGGGCCGGCCAGGTAGGACGCATCAGCCAAACGCAGGAAACCGAAACCa                                                                                                                          >  7:15085/1‑148 (MQ=255)                gCTCCCCTTCCTTCGGTGGGCGGATCTTGCCGACGATGGTGTCGCCGGTACGCAGGTTGAAACGGCGGATCTGGCTGGGCGAGACATAGATATCGTCTGGGCCGGCCAGGTAGGACGCATCAGCCAAACGCAGGAAACCGAAACCATc                                                                                                                        <  7:126031/148‑1 (MQ=255)                  tCCCCTTCCTTCGGTGGGCGGATCTTGCCGACGATGGTGTCGCCGGTACGCAGGTTGAAACGGCGGATCTGGCTGGGCGAGACATAGATATCGTCTGGGCCGGCCAGGTAGGACGCATCAGCCAAACGCAGGAAACCGAAACCATCCt                                                                                                                      >  7:89803/1‑148 (MQ=255)                     ccttccttCGGTGGGCGGATCTTGCCGACGATGGTGTCGCCGGTACGCAGGTTGAAACGGCGGATCTGGCTGGGCGAGACATAGATATCGTCTGGGCCGGCCAGGTAGGACGCATCAGCCAAACGCAGGAAACCGAAACCATCCTGga                                                                                                                   <  6:65234/148‑1 (MQ=255)                           ttcGGTGGGCGGATCTTGCCGACGATGGTGTCGCCGGTACGCAGGTTGAAACGGCGGATCTGGCTGGGCGAGACATAGATATCGTCTGGGCCGGCCAGGTAGGACGCATCAGCCAAACGCAGGAAACCGAAACCATCCTGGAGAAtc                                                                                                              <  3:63432/147‑1 (MQ=255)                               gTGGGCGGATCTTGCCGACGATGGTGTCGCCGGTACGCAGGTTGAAACGGCGGATCTGGCTGGGCGAGACATAGATATCGTCTGGGCCGGCCAGGTAGGACGCATCAGCCAAACGCAGGAAACCGAAACCATCCTGGAGAATCTCCAg                                                                                                         <  2:100184/148‑1 (MQ=255)                                                  gaTGGTGTCGCCGGTACGCAGGTTGAAACGGCGGATCTGGCTGGGCGAGACATAGATATCGTCTGGGCCGGCCAGGTAGGACGCATCAGCCAAACGCAGGAAACCGAAACCATCCTGGAGAATCTCCAGCACGCCGTCACCCGAGAtc                                                                                      >  3:124626/1‑148 (MQ=255)                                                           gCCGGTACGCAGGTTGAAACGGCGGATCTGGCTGGGCGAGACATAGATATCGTCTGGGCCGGCCAGGTAGGACGCATCAGCCAAACGCAGGAAACCGAAACCATCCTGGAGAATCTCCAGCACGCCGTCACCCGAGATCTCTTcgccg                                                                             >  5:90940/1‑148 (MQ=255)                                                                     aGGTTGAAACGGCGGATCTGGCTGGGCGAGACATAGATATCGTCTGGGCCGGCCAGGTAGGACGCATCAGCCAAACGCAGGAAACCGAAACCATCCTGGAGAATCTCCAGCACGCCGTCACCCGAGATCTCTTCGCCGCTTTTCGCAt                                                                   >  6:25454/1‑148 (MQ=255)                                                                        ttGAAACGGCGGATCTGGCTGGGCGAGACATAGATATCGTCTGGGCCGGCCAGGTAGGACGCATCAGCCAAACGCAGGAAACCGAAACCATCCTGGAGAATCTCCAGCACGCCGTCACCCGAGATCTCTTCGCCGCTTTTCGCATGct                                                                >  1:101881/1‑148 (MQ=255)                                                                           aaaCGGCGGATCTGTCTGGTCGAGACATAGATATCGTCTGGGCCGGCCAGGTAGGACGCATCAGCCAAACGCAGGAAACCGAAACCATCCTGGAGAATCTCCAGCACGCCGTCACCCGAGATCTCTTCGCCGCTTTTCGCATGcttc                                                              <  4:150462/147‑1 (MQ=255)                                                                           aaaCGGCGGATCTGGCTGAGCGAGACATAGATATCGTCTGGGCCGGCCAGGTAGGACGCATCAGCCAAACGCAGGAAACAGAAACCATCCTGGAGAATCTCCAGCACTACGTCACCAGAGATCTCTTCGCAGCTTTTCGCATGcttc                                                              >  7:127944/1‑147 (MQ=255)                                                                            aaCGGCGGATCTGGCTGGGCGAGACATAGATATCGTCTGGGCCGGCCAGGTAGGACGCATCAGCCAAACGCAGGAAACCGAAACCATCCTGGAGAATCTCCAGCACGCCGTCACCCGAGATCTCTTCGCCGCTTTTCGCATGcttctt                                                            <  1:106790/148‑1 (MQ=255)                                                                                        ggctggGCGAGACATAGATATCGTCTGGGCCGGCCAGGTAGGACGCATCAGCCAAACGCAGGAAACCGAAACCATCCTGGAGAATCTCCAGCACGCCGTCACCCGAGATCTCTTCGCCGCTTTTCGCATGCTTCTTCAGCAGGGCGa                                                 >  4:63896/1‑147 (MQ=255)                                                                                                     aTAGATATCGTCTGGGCCGGCCAGGTAGGACGCATCAGCCAAACGCAGGAAACCGAAACCATCCTGGAGAATCTCCAGCACGCCGTCACCCGAGATCTCTTCGCCGCTTTTCGCATGCTTCTTCAGCAGGGCGAAAATCACGTCCTGt                                   <  6:90940/148‑1 (MQ=255)                                                                                                     aTAGATATCGTCTGGGCCGGCCAGGTAGGACGCATCAGCCAAACGCAGGAAACCGAAACCATCCTGGAGAATCTCCAGCACGCCGTCACCCGAGATCTCTTCGCCGCTTTTCGCATGCTTCTTCAGCAGGGCGAAAATCACGTCCTGt                                   >  4:89910/1‑148 (MQ=255)                                                                                                         atctCGTCTGGGCCGGCCAGGTAGGACGCATCAGCCAAACGCAGGAAACCGAAACCATCCTGGAGAATCTCCAGCACGCCGGCACCCGAGATCTCTTCGCCGCTTTTCGCATGCTTCTTCAGCAGGGCGAAAATCACGTCCTGTTTgc                               <  5:65209/145‑1 (MQ=255)                                                                                                         atatAGTCTGGGCCGGCCAGTTAGGAAGCATAAGCCAAACTCAGGAAACCGAAACCATCCTGGAGAATCTCCAGCACGCCGTCACCCGAGATCTCTTCGCCGCTTTTCGCATTCTTCTTAAGCAGGGCGAAAATCACGTCCTGTTTgc                               >  4:123002/1‑148 (MQ=255)                                                                                                          tatCGTCTGGGCCGGCCAGGTAGGACGCATCAGCCAAACGCAGGAAACCGAAACCATCCTGGAGAATCTCCAGCACGCCGTCACCCGAGATCTCTTCGCCGCTTTTCGCATGCTTCTTCAGCAGGGCGAAAATCACGTCCTGTTTg                                >  4:37948/1‑146 (MQ=255)                                                                                                           atCGTCTGGGCCGGCCAGGTAGGACGCATCAGCCAAACGCAGGAAACCGAACCCATCCTGGAGAATCTCCAGCACGCCGTCACCCGAGATCTCTTCGCCGCTTTTCGCATGCTTCTTCAGCAGGGCGAAAATCACGTCCTGTTTgc                               <  3:37948/146‑1 (MQ=255)                                                                                                                            ggTAGGACGCATCAGCCAAACGCAGGAAACCGAAACCATCCTGGAGAATCTCCAGCACGCCGTCACCCGAGATCTCTTCGCCGCTTTTCGCATGCTTCTTCAGCAGGGCGAAAATCACGTCCTGTTTGCGCGAACGGGCCATGTTTTc            <  1:127339/148‑1 (MQ=255)                                                                                                                               aGGACGCATCAGCCAAACGCAGGAAACCGAAACCATCCTGGAGAATCTCCAGCACGCCGTCACCCGAGATCTCTTCGCCGCTTTTCGCATGCTTCTTCAGCAGGGCGAAAATCACGTCCTGTTTGCGCGAACGGGCCATGTTTTCGAt         <  8:15085/148‑1 (MQ=255)                                                                                                                                 gACGCATCAGCCAAACGCAGGAAACCGAAACCATCCTGGAGAATGTCCAGCACGCCGTCACCCGAGATCTCTTCGCCGCTTTTCGCATGCTTCTTCAGCAGGGCGAAAATCACGTCCTGTTTGCGCGAACGGGCCATGTTTTCGATg        <  7:119356/147‑1 (MQ=255)                                                                                                                                    gCATCAGCCAAACGCAGGAAACCGAAACCATCCTGGAGAATCTCCAGCACGCCGTCACCCGAGATCTCTTCGCCGCTTTTCGCATGCTTCTTCAGCAGGGCGAAAATCACGTCCTGTTTGCGCGAACGGGCCATGTTTTCGATGCCCa    >  5:38315/1‑148 (MQ=255)                                                                                                                                      aTCAGCCAAACGCAGGAAACCGAAACCATCCTGGAGAATCTCCAGCACGCCGTCACCCGAGATCTCTTCGCCGCTTTTCGCATGCTTCTTCAGCAGGGCGAAAATCACGTCCTGTTTGCGCGAACGGGCCATGTTTTCGATGCCCATc  >  5:161037/1‑148 (MQ=255)                                                                                                                                             |                                                                                                                                             GCAGGGCGAAGTAACGCTCCCCTTCCTTCGGTGGGCGGATCTTGCCGACGATGGTGTCGCCGGTACGCAGGTTGAAACGGCGGATCTGGCTGGGCGAGACATAGATATCGTCTGGGCCGGCCAGGTAGGACGCATCAGCCGAACGCAGGAAACCGAAACCATCCTGGAGAATCTCCAGCACGCCGTCACCCGAGATCTCTTCGCCGCTTTTCGCATGCTTCTTCAGCAGGGCGAAAATCACGTCCTGTTTGCGCGAACGGGCCATGTTTTCGATGCCCATC  >  NC_002947/5948134‑5948414

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑