Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_002947 4,740,811 (T)7→6 intergenic (+48/+705) gltA → / ← yeiW citrate synthase/putative oxidoreductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,740,8050T.95.3% 174.2 / 2.5 43intergenic (+42/+711)gltA/yeiWcitrate synthase/putative oxidoreductase
Reads supporting (aligned to +/- strand):  ref base T (0/2);  new base . (23/18);  total (23/20)
Fisher's exact test for biased strand distribution p-value = 2.10e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.60e-01

CGCACTGGAAAGAAATGCTCTCCAGCCCGTACAAGATCGGCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGT‑AGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCCTTTTTTTATTTTTCTGGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGCGCATCCACCACGAACTT  >  NC_002947/4740657‑4740943
                                                                                                                                                     |                                                                                                                                          
cGCACTGGAAAGAAATGCTCTCCAGCCCGTACAAGATCGGCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGT‑AGCCGAACGCAAGAAGGCTGCCCTAGGGCAGCCt                                                                                                                                            >  1:94071/1‑149 (MQ=255)
 gCACTGGAAAGAAATGCTCTCCAGCCCGTACAAGATCGGCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGT‑AGCCGAACGCAAGAAGGCTGCCCGAGGGCAGCCt                                                                                                                                            <  3:111431/148‑1 (MQ=255)
  cACTGGAAAGAAATGCTCTCCAGCCCGTACAAGATCGGCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGT‑AGCCGAACGCAAAAAGGCTGCCCGAGGGCAGCCttc                                                                                                                                          <  2:2352/149‑2 (MQ=255)
     tGGAAAGAAATGCTCTCCAGCCCGTACAAGATCGGCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGT‑AGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCCttttt                                                                                                                                        >  4:86020/1‑148 (MQ=255)
       gaaagaaaTGCTCTCCAGCCCGTACAAGATCGGCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGT‑AGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCCttttttat                                                                                                                                     <  3:243710/149‑3 (MQ=255)
       gaaagaaaTGCTCTCCAGCCCGTACAAGATCGGCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGT‑AGCCGAACGCAAAAAGGCTGCCCGAGGGCAGCcttcttgc                                                                                                                                      <  4:404189/148‑3 (MQ=255)
            aaaTGCTCTCCAGCCCGTACAAGATCGGCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGT‑AGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCCttttttatttgtc                                                                                                                                <  4:19540/149‑8 (MQ=255)
                    tcCAGCCCGTACAAGATCGGCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGT‑AGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCg                                                                                                                       >  4:115347/1‑149 (MQ=255)
                    tcCAGCCCGTACAAGATCGGCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGT‑AGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCg                                                                                                                       >  5:17486/1‑149 (MQ=255)
                         cccGTACAAGATCGGCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGT‑AGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCctc                                                                                                                  <  3:115347/149‑1 (MQ=255)
                               cAAGATCGGCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGT‑AGACGAACGCAAGAACGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCCGCCTCATcgcg                                                                                                            >  4:395148/1‑149 (MQ=255)
                                aaGATCGGCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGAGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGT‑AGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCCttttttatt                                                                                                                                    <  4:180115/125‑4 (MQ=255)
                                aaGATCGGCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGAGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGT‑AGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCCttttttatt                                                                                                                                    >  3:180115/1‑122 (MQ=255)
                                    tCGGCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGT‑AGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCtt                                                                                                       <  3:86020/149‑1 (MQ=255)
                                          gTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGT‑AGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCcgc                                                                                                 <  6:17486/149‑1 (MQ=255)
                                             cgcgCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGT‑AGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGaa                                                                                              <  2:94071/149‑1 (MQ=255)
                                              gcgcCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGT‑AGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCcgcg                                                                                                >  6:119484/1‑146 (MQ=255)
                                              gcgcCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGT‑AGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAag                                                                                             <  4:204409/149‑1 (MQ=255)
                                                gcCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGT‑AGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGGCAAGGCCGCGAagag                                                                                           <  8:171364/149‑1 (MQ=38)
                                                  cAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGT‑AGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGc                                                                                         >  7:263049/1‑149 (MQ=255)
                                                           acCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGT‑AGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGc                                                                                                                     <  6:2797/112‑1 (MQ=255)
                                                           acCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGT‑AGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGc                                                                                                                     >  5:2797/1‑112 (MQ=255)
                                                                     aGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGT‑AGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACaaaa                                                                      >  4:159963/1‑149 (MQ=255)
                                                                         gcgcGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGT‑AGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGGCAAGGCCGCGAaga                                                                                            <  6:224044/123‑1 (MQ=37)
                                                                         gcgcGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGT‑AGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGGCAAGGCCGCGAaga                                                                                            >  5:224044/1‑123 (MQ=37)
                                                                          cgcgACATCGTTGCCCTGAAACACCGCAAGTAAGCTGCAGT‑AGCCGAACGCAAGACGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCa                                                                 <  6:193795/149‑1 (MQ=255)
                                                                              aCATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGT‑AGCGGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTAGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGttt                                                             >  3:294169/1‑149 (MQ=255)
                                                                               cATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGT‑AGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCGCAGtttt                                                            >  5:397254/1‑149 (MQ=255)
                                                                                   gTTGCCCTGAAAGACCGCAAGTAAGCTGCAGT‑AGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTtctc                                                        >  6:216729/1‑149 (MQ=255)
                                                                                    ttGCCCTGAAAGACCGCACGTAAGCTCCTGT‑AGCCTACCGCAAGACGGCTGCCCTCGGGAAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCTAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTcc                                                       <  6:397254/149‑1 (MQ=255)
                                                                                    ttGCCCTGAAAGACCGCAAGTAAGCTGCAGT‑AGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTcc                                                       >  7:325111/1‑149 (MQ=255)
                                                                                       cccTGAAAGACCGCAAGTAAGCTGCAGT‑AGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTccgc                                                     <  5:119484/148‑1 (MQ=255)
                                                                                       cccTCAAACACCGCAATTAAGCTGCAGT‑ACCCGACCGCAAGACGGCTGCCCTCGGGCAGCGTTTTTTT‑TTTGTCCGGTCCCGGCCTCTGCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTccgcc                                                    <  4:37892/149‑1 (MQ=255)
                                                                                        ccTGAAAGACCGCAAGTAAGCTGCAGTGAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTATTAGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCACAGTTTTTCTacgcc                                                    >  1:235805/1‑149 (MQ=255)
                                                                                         cTGAAAGACCGCAAGTAAGCTGCAGT‑AGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCATCGCGGCCTTGCCCGCGAACAGGCCGGCACAGACACCCCAAAACCACAGTTTTTATccgcccg                                                  >  4:51056/1‑149 (MQ=255)
                                                                                          tGAAAGACCGCAAGTAAGCTGCAGT‑AGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTccgcccgc                                                 >  1:275125/1‑149 (MQ=255)
                                                                                              tgACCGCATGTAAGCTGCAGT‑AGCCTAACGCAATAAGGCTGCCCTGGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCAc                                             <  4:294169/148‑1 (MQ=255)
                                                                                                aCCGCAAGTAAGCTGCAGT‑AGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACg                                            >  4:280729/1‑148 (MQ=255)
                                                                                                  cGCAAGTAAGCTGCAGT‑AGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTAGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCACAGTTTTTCTCCGCCCGCTCACg                                            >  3:303926/1‑146 (MQ=255)
                                                                                                   gCAAGTAAGCTGCAGT‑AGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGc                                        <  8:325111/149‑1 (MQ=255)
                                                                                                     aaGTAAGCTGCAGT‑AGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTTCCCGCGAATAGGCCCGCACAGACACCACAAAACCTAAGttt                                                             >  7:202070/1‑126 (MQ=255)
                                                                                                     aaGTAAGCTGCAGT‑AGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTAGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAATTTTTCTCCGCCCCCTCCCGCAcccc                                      >  4:234653/1‑149 (MQ=255)
                                                                                                                aGT‑AGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGCCACCCCAAAACCTCAGTTTTTATACGCCCGCTCACGCAGccc                                      >  5:330623/1‑138 (MQ=38)
                                                                                                                 cttccccGAACGCAAGAAGCCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGATGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGccc                                      <  6:330623/133‑1 (MQ=255)
                                                                                                                   tagcgtaaCGCAAGAAGGGTGCGCGGGGGTAGCCTTTTTAT‑TTTGTCCTGTCCGGGCGTCTTCGCGGCCTTGCCCGCTATGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTAt                           <  3:395148/141‑1 (MQ=255)
                                                                                                                            gCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGCGCa                <  2:275125/149‑1 (MQ=255)
                                                                                                                              aagaagGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGCGCATc              >  8:199168/1‑149 (MQ=255)
                                                                                                                                 aagGCTGCCCGAGGGCAGCC‑TTCTTTATTTGTCCTGTGCCGGCCTCATCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCACTTTTTATCCGCCCCCTCCCGCAGCCCTTTAACGGGATTGAACGGCGCATccac           >  2:232300/1‑149 (MQ=255)
                                                                                                                                  agGCTGCCCTGGGGCTGCC‑TTTTTTATTTGTCCTGTGCCGGCCGCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGCGCATccacc          <  3:51056/149‑1 (MQ=255)
                                                                                                                                   ggCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGCGCATccacca         <  2:117451/149‑1 (MQ=255)
                                                                                                                                        cccTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTGCGGGGCCTTCGCCGCGATGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGCGCATCCACCACGaa     <  4:116041/148‑1 (MQ=255)
                                                                                                                                          cTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTGTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGCAAGGCGCATCCACCACGAACtt  >  3:40665/1‑149 (MQ=255)
                                                                                                                                                     |                                                                                                                                          
CGCACTGGAAAGAAATGCTCTCCAGCCCGTACAAGATCGGCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGT‑AGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCCTTTTTTTATTTTTCTGGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGCGCATCCACCACGAACTT  >  NC_002947/4740657‑4740943

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑