Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_002947	336,125	+T	coding (210/237 nt)	PP_0278 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	336,124	1	.	T	98.4%	216.6 / ‑3.2	64	coding (209/237 nt)	PP_0278	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base . (1/0);  new base T (33/30);  total (34/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.31e-01

ACCCATCCCTCAATCTCCTACGCGTAGAGCCCGGCGTCCCCGTCGACGCGGCCTACGAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCT‑GGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGA‑T‑ACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCATTTGTGGGAGCGGCCTTGCGTCGCGAATGGGCTGCAAAGCAGCCCCGGCGATTTCAGA  >  NC_002947/335980‑336263                                                                                                                                                   |                                                                                                                                             aCCCATCCCTCAATCTCCTACGCGTAGAGCCCGGCGTCCCCGTCGACGCGGCCTACGAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCT‑GGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGA‑ttac                                                                                                                                          <  1:390445/149‑4 (MQ=255) aCCCATCCCTCAATCTCCTACGCGTAGAGCCCGGCGTCCCCGTCGACGCGGCCTACGAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCT‑GGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGA‑ttac                                                                                                                                          >  6:81431/1‑146 (MQ=255)   ccATCCCTCAAGCTCCTACCCGTAGAGCCCGGCGTCCCCGCCGACGCGGCCTACGAGCATCTCTCGATCCAGATGGGCTACATCAAGCACCT‑GGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGA‑ttacct                                                                                                                                        <  8:304691/149‑6 (MQ=255)        ccTCAATCTCCTACGCGTATAGCCCGCCGTCCCCTTCGACGCGGCCTACGAGCATTTCTCGATCCTGATGCTCTACATCAAGCACCT‑GGTGCGCGAGGGCGATATGGAGGACGACCATAATTTCCTGGGGGCAGCCGATT‑ACCTGTGCg                                                                                                                                   <  8:377389/149‑1 (MQ=255)        ccTCAATCTCCTACGCGTACCCCCCCGCCTCCCCGTCCACGCGGCCTACGAGCATGTCTCCATCCTGATGGGCTACATCAAGCACCT‑GGTGCGCGAGGGCTATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATT‑ACCTGTGCg                                                                                                                                   <  8:383076/149‑1 (MQ=255)         cTCAATCTCCTACGCGTAGAGCCCGGCGTCCCCGTCGACGCGGCCTACGAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCT‑GGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATT‑ACCTGTGCg                                                                                                                                   >  7:386945/1‑148 (MQ=255)          tCAATCTCCTACGCGTAGAGCCCGGCGTCCCCGTCGACGCGGCCTACGAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCT‑GGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATT‑ACCTGTGCGa                                                                                                                                  >  4:252173/1‑148 (MQ=255)            aaTCTCCTACGCGTAGAGCCCGGCGTCCCCGTCGACGCGGCCTACGAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCT‑GGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATT‑ACCTGTGCGACAt                                                                                                                               >  6:78825/1‑149 (MQ=255)                tcCTACGCGTAGAGCCCGGCGTCCCCGTCGACGCGGCCTACGAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCT‑GGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATT‑ACCTGTGCGACAt                                                                                                                               >  5:71380/1‑145 (MQ=255)                tcCTACGCGTAGAGCCCGGCGTCCCCGTCGACGCGGCCTACGAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCT‑GGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATT‑ACCTGTGCGACAt                                                                                                                               <  6:71380/145‑1 (MQ=255)                tcCTACGCGTAGAGCCCGGCGTCCCCGTCGACGCGGCCTACGAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCT‑GGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATT‑ACCTGTGCGACATGGc                                                                                                                            >  5:120474/1‑148 (MQ=255)                       cgTAGAGCCCGGCGTCCCCGTCGACGCGGCCTACGAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCT‑GGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATT‑ACCTGTGCGACATGGCCAAGgcgc                                                                                                                    <  1:201804/149‑1 (MQ=255)                           gagCCCGGCGTCCCCGTCGACGCGGCCTACGAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCT‑GGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATT‑ACCTGTGCGACATGGCCAAGGCGCtga                                                                                                                 <  1:240064/148‑1 (MQ=255)                                   cGTCCCCGTCGACGCGGCCTACGAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCT‑GGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATT‑ACCTGTGCGACATGGCCAAGGCGCTGATGAACGata                                                                                                        <  8:386945/149‑1 (MQ=255)                                      ccccGTCGACGCGGCCTACGAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCT‑GGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATT‑ACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATc                                                                                                      >  5:313044/1‑148 (MQ=255)                                        ccGTCGACGCGGCCTACGAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCT‑GGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATT‑ACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATc                                                                                                      >  7:196719/1‑146 (MQ=255)                                         cGTCGACGCGGCCTACGAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCT‑GGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATT‑ACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATc                                                                                                      >  6:336859/1‑145 (MQ=255)                                         cGTCGACGCGGCCTACGAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCT‑GGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATT‑ACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATc                                                                                                      >  6:107382/1‑145 (MQ=255)                                            cGACGCGGCCTACGAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCTGGGAGCGCGAGGGCGATATGGAGGCCGACCATAAATTCCTGGGGGCAGCCGA‑TAACCTGTGCGACATGGCCAAGGCGCTGATGAACCAGATCggaaca                                                                                                >  1:111379/1‑144 (MQ=255)                                              aCGCGGCCTACGAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCT‑GGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATT‑ACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGcc                                                                                             <  2:270158/149‑1 (MQ=255)                                                gcgGCCTACGAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCT‑GGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATT‑ACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCaa                                                                                           <  2:276278/149‑1 (MQ=255)                                                    ccTACGAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCT‑GGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATT‑ACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAggg                                                                                        >  8:97979/1‑148 (MQ=255)                                                           gCATGTCTCGATCCTGATGGGCTACATCAAGCACCT‑GGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATT‑ACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGgcg                                                                                 >  3:407019/1‑148 (MQ=255)                                                                    gATCCTGATGGGCTACATCAAGCACCT‑GGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATT‑ACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGc                                                                         <  3:252173/147‑1 (MQ=255)                                                                     aTCCTGATGGGCTACATCAAGCACCT‑GGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATT‑ACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCAtt                                                                      >  2:197923/1‑149 (MQ=255)                                                                       ccTGATGGGCTACATCAAGCACCT‑GGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATT‑ACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGa                                                                    >  1:212905/1‑149 (MQ=255)                                                                         tGATGGGCTACATCAAGCACCT‑GGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATT‑ACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGAcg                                                                  >  3:311561/1‑149 (MQ=255)                                                                           aTGGGCTACATCAAGCACCT‑GGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATT‑ACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGAcg                                                                  <  1:197923/147‑1 (MQ=255)                                                                                 tACATCAAGCACCT‑GGTGCGCGAGGGCGATATGGAAGACCACCAAAAATTCCTGGCGGCAGCCGATT‑ACCTGTGCGACATGGCCAAGGCGCAGATGAACGATATCGAGATCGCCAAGCGTAAGCCGCATTAGGCATTGACGCGGTCaat                                                          >  1:64879/1‑147 (MQ=255)                                                                                 tACATCAAGCACCT‑GGTGCGCGAGGACGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATT‑ACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCAtt                                                          >  3:132695/1‑149 (MQ=255)                                                                                   cATCAAGCACCT‑GGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATT‑ACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCATTtg                                                        >  1:154265/1‑149 (MQ=255)                                                                                    aTCAAGCACCT‑GGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATT‑ACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATGAGGCATTGACGCGGTCATTtgt                                                       >  2:16021/1‑149 (MQ=255)                                                                                     tCAAGCACCT‑GGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATT‑ACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCATTtgtg                                                      <  7:97979/149‑1 (MQ=255)                                                                                       aaGCACCT‑TGTGAGCGAGGGCGATATGGATTACGAACATAAATTCCTGGGGGCAGCAGATT‑ACCTGTGCGACATGGCCAAGGCGCTGTTGAACGATATCGAGATCGGCAAGGGTATGGCGCATTAGGCATTTACGCTGTCAttt                                                         <  6:404714/144‑1 (MQ=255)                                                                                       aaGCACCT‑GGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATT‑ACCTGTGCGACATGGCCAGGGCGCTGATGAACGAGATCGAGATCGCCAAGGGTAAGGCGCTTTAGGCATTGACGGGGTCAGTTGTggg                                                    >  8:144603/1‑149 (MQ=255)                                                                                       aaGCACCT‑GGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATT‑ACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAg                                                                           <  4:364078/126‑1 (MQ=255)                                                                                       aaGCACCT‑GGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATT‑ACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAg                                                                           >  3:364078/1‑126 (MQ=255)                                                                                       aaGCACCT‑GGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATT‑ACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCATTTGTgg                                                     <  5:107382/148‑1 (MQ=255)                                                                                       aaGCACCT‑GGTCCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATT‑ACCTGAGCGACATGGCCAAGGCGCTGATGAACGATATAGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCAttt                                                         >  5:404714/1‑144 (MQ=255)                                                                                        aGCACCT‑GGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATT‑ACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCATTTGTgg                                                     >  3:100498/1‑147 (MQ=255)                                                                                         gCACCT‑GGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATT‑ACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCATTTGTGGGAg                                                  >  5:381607/1‑149 (MQ=255)                                                                                         gCACCT‑GGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATT‑ACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCATTTGTGGGAg                                                  >  7:265299/1‑149 (MQ=255)                                                                                                 gTGCGCGAGGACGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATT‑ACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCATTTGTGGGAGCGGCCtt                                           <  4:132695/149‑1 (MQ=255)                                                                                                    cgcgAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATT‑ACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCATTTGTGGGAGCGGCCTTGc                                         <  6:120474/148‑1 (MQ=255)                                                                                                     gcgAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATT‑ACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCATTTGTGGGAGCGGCCTTGCGt                                       <  5:78825/149‑1 (MQ=255)                                                                                                        aGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATT‑ACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCATTTGTGGGAGCGGCCTTGCGTcgc                                    <  6:313044/149‑1 (MQ=255)                                                                                                            cGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATT‑ACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCATTTGTGGGAGCGGCCTTGCGTCGCGAAt                                >  2:398523/1‑149 (MQ=255)                                                                                                               tatGGAGGACGACCATAAATTCCTGGGGGCAGCCGATT‑ACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCATTTGTGGGAGCGGCCTTGCGTCGCGAATggg                             <  6:381607/149‑1 (MQ=255)                                                                                                                      gacgacCATAAATTCCTGGGGGCAGCCGATT‑ACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCATTTGTGGGAGCGGCCTTGCGTCGCGAATGGGCt                           >  2:182065/1‑144 (MQ=255)                                                                                                                      gacgacCATAAATTCCTGGGGGCAGCCGATT‑ACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCATTTGTGGGAGCGGCCTTGCGTCGCGAATGGGCt                           <  1:182065/144‑1 (MQ=255)                                                                                                                      gacgacCATAAATTCCTGGGGGCAGCCGATT‑ACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCATTTGTGGGAGCGGCCTTGCGTCGCGAATGGGCTGCaaa                      <  8:265299/149‑1 (MQ=255)                                                                                                                         gacCATAAATTCCTGGGGGCAGCCGATT‑ACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCATTTGTGGGAGCGGCCTTGCGt                                       >  2:373974/1‑129 (MQ=255)                                                                                                                         gacCATAAATTCCTGGGGGCAGCCGATT‑ACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCATTTGTGGGAGCGGCCTTGCGt                                       <  1:373974/129‑1 (MQ=255)                                                                                                                           ccATAAATTCCTGGGGGCAGCCGATT‑ACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCAt                                                                              >  7:182477/1‑88 (MQ=255)                                                                                                                           ccATAAATTCCTGGGGGCAGCCGATT‑ACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCAt                                                                              <  8:182477/88‑1 (MQ=255)                                                                                                                            cATAAATTCCTGGGGGCAGCCGATT‑ACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCATTTGTGGGAGCGGCCtt                                           >  2:316695/1‑122 (MQ=255)                                                                                                                            cATAAATTCCTGGGGGCAGCCGATT‑ACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCATTTGTGGGAGCGGCCtt                                           <  1:316695/122‑1 (MQ=255)                                                                                                                            cATAAATTCCTGGGGGCAGCCGATT‑ACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCATTTGTGGGAGCGGCCTTGCGTCGCGAATGGGCTGCAAAGCAGcc                <  2:338681/149‑1 (MQ=255)                                                                                                                            cATAAATTCCTGGGGGCAGCCGATT‑ACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCATTTGTGGGAGCGGCCTTGCGTCGCGAATGGGCTGCAAAGCAGcc                >  1:338681/1‑149 (MQ=255)                                                                                                                               aaaTTCCTGGGGGCAGCCGATT‑ACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCATTTGTGGGAGCGGCCTTGCGTCGCGCATGGGGTGCAAATCCGccccc             >  5:81779/1‑148 (MQ=255)                                                                                                                               aaaTTCCTGGGGGCAGCCGATT‑ACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCAAAGGGTAAGGCGCATTAGGCATTGACGCGGTCATTTGTGGGAGCGGCCTTGCGTCGCGAATGGGCTGCAAAGCAGCCCCg             <  2:212905/149‑1 (MQ=255)                                                                                                                                taTTCGTGGGGGCAGCCTATT‑ACCTGTGCGACATGGCCAAGGGGCTGATGAACGATATCGTGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCATTTGTGGGAGCGGCCTTGCGTCGCGAATGGGCTGCAAAGCAGCCCCgg            <  6:240699/148‑1 (MQ=38)                                                                                                                                  ttCCTGGGGGCAGCCGATT‑ACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCATTTGTGGGAGCGGCCTTGCGTCGCGAATGGGCTGCAAAGCAGCCCCgg            >  5:240699/1‑147 (MQ=255)                                                                                                                                   tCCTGGGGGCAGCCGATT‑ACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCATTTGTGGGAGCGGCCTTGCGTCGCGAATGGGCTGc                         <  4:142954/133‑1 (MQ=255)                                                                                                                                   tCCTGGGGGCAGCCGATT‑ACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCATTTGTGGGAGCGGCCTTGCGTCGCGAATGGGCTGc                         >  3:142954/1‑133 (MQ=255)                                                                                                                                         gggCAGCCGATT‑ACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCATTTGTGGGAGCGGCCTTGCGTCGCGAATGGGCTGCAAAGCAGCCCCGGCGATTTCag   <  5:336859/149‑1 (MQ=255)                                                                                                                                          ggCAGCCGATT‑ACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCATTTGTGGGAGCGGCCTTGCGTCGCGAATGGGCTGCAAAGCAGCCCCGGCGATTTCaga  <  4:407019/149‑1 (MQ=255)                                                                                                                                                   |                                                                                                                                             ACCCATCCCTCAATCTCCTACGCGTAGAGCCCGGCGTCCCCGTCGACGCGGCCTACGAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCT‑GGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGA‑T‑ACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCATTTGTGGGAGCGGCCTTGCGTCGCGAATGGGCTGCAAAGCAGCCCCGGCGATTTCAGA  >  NC_002947/335980‑336263

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑