Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC	NC_002947	5,555,608	+GCC	intergenic (‑84/+87)	PP_4887 ← / ← PP_4888	hypothetical protein/methyl‑accepting chemotaxis transducer

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_002947	= 5555608	1 (0.010)	50 (0.700) +GCC	40/276	1.3	98.1%	intergenic (‑84/+87)	PP_4887/PP_4888	hypothetical protein/methyl‑accepting chemotaxis transducer
	?	NC_002947	5555609 =	1 (0.010)					intergenic (‑85/+86)	PP_4887/PP_4888	hypothetical protein/methyl‑accepting chemotaxis transducer

GCTGTCACTGTGCAGGAAATTCAGGTCGGCCTGGGCAGCGATTAGTCGGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGC‑GAAAGGGCCGCATAGCG‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_002947/5555462‑5555608 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑CCGGCAATTTCCGCGTGTATGCAACAAACCTGGGGCTGCTATGCAGCCCTTTCGCGACACAAGGCCCACAAAGGACCGCGCAGGCTTCAGGCCCGGAACTGGCCCAGGCTGGCGCGCAGCTGAGCCGCCAGATCATCC  >  NC_002947/5555609‑5555746                                                                                                                                                     |||                                                                                                                                           GCTGTCACTGTGCAGGAAATTCAGGTCGGCCTGGGCAGCGATTAGTCGGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGC‑GAAAGGGCCGCATAGCGGC                                                                                                                                             >  2:27785/1‑149   TGTCACTGTGCAGGAAATTCAGGTCGGCCTGGGCAGCGATTAGTCGGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGC‑GAAAAGGCCGCATAGCGGCCC                                                                                                                                           >  3:25563/1‑149       ACTGTGCAGGAAATTCAGGTCGGCCTGGGCAGCGATTAGTCGGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGC‑GCAAGGGCCGCATTGGGGCCCCGG                                                                                                                                        >  2:272553/1‑148          GTGCAGGAAATTCAGGTCGGCCTGGGCAGCGATTAGTCGGGCGGACATGAGCAACTACTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGC‑GAAAGGGCCGCATAGCGGCCCCGGCAAT                                                                                                                                    >  1:331572/1‑149           TGCAGGAAATTCAGGTCGGCCTGGGCAGCGATTAGTCGGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGC‑TAAAGGGCCGCATAGCGGCCCCGGCAA                                                                                                                                     <  3:196387/147‑1             CAGGAAATTCAGGTCGGCCTGGGCAGCGATTAGTCGGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGC‑GAAAGGGCCGCATAGCGGCCCCGGCAATTTC                                                                                                                                 <  7:54898/149‑1               GGAAATTCAGGTCGGCCTGGGCAGCGATTAGTCGGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGC‑GAAAGGGCCGCATAGCGGCCCCGGCAATTTCCG                                                                                                                               >  4:312260/1‑149                   ATTCAGGTCGGCCTGGGCAGCGATTAGTCGGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGC‑GAAAGGGCCGCATAGCGGCCCCGGCAATTTCCGCGTG                                                                                                                           >  6:98192/1‑149                      CAGGTCGGCCTGGGCAGCGATTAGTCGGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGC‑GAAAGGGCCGCATAGCGGCCCCGGCAATTTCCGCGTGTA                                                                                                                         <  3:330002/148‑1                        GGTCGGCCTGGGCAGCGATTAGTCGGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGTAGCGGCCTTGTGTCGC‑GAAAGGGCCGAATAGCGGCCCCGGCAATTTCCGCGTGTATGC                                                                                                                      <  4:156618/149‑1                         GTCGGCCTGGGCAGCGATTAGTCGGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTGGCGGAAAGGGCCCCAT‑GCGGGCCCGGCCCTTTTCGCGgggtttg                                                                                                                      >  7:341941/1‑141                                  gtCAGCGATTAGTCGGGCGGAGTTGAGCAAGTGCTTTTGTCATGTGTCGCGGACAGCATAGCAACACCGGTGAGGTGTGGGAGCGGCCTTGTGTCGG‑GAGAGGGCCGCATTGCGGCCCCGGCAATTTCCGCGTGTATGCAACAAACCTG                                                                                                            <  2:179074/147‑1                                  GGCAGCGATTAGTCGGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGC‑GAAAGGGCCGCATAGCGGCCCCGGCAATTTCCGCGTGTATGCAACAAACCT                                                                                                             <  6:8226/148‑1                                        GATTAGTCGGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGC‑GAAAGGGCCGCATAGCGGCCCCGGCAATTTCCGCGTGTATGCAACAAACCTGGGGCTG                                                                                                      <  5:98192/149‑1                                                          GAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGC‑GAAAGGGCCGCATAGCGGCCCCGGCAATTTCCGCGTGTATGC                                                                                                                      <  6:109993/115‑1                                                          GAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGC‑GAAAGGGCCGCATAGCGGCCCCGGCAATTTCCGCGTGTATGC                                                                                                                      >  5:109993/1‑115                                                          GAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTAGC‑GAAAGCGCCCCATTGCGGCCCCCGCAATTTACGCCTGGATGCAACCAACCTGGGGCGGCGATGCAGCCCATACGCG                                                                                    >  2:36273/1‑149                                                              AACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGC‑GAAAGGGCCGCATAGCGGCCCCGGCAATTTCCGCGTGTATGCAACAAACCTGGGGCTGCTATGCAGCCCTTTCGCGACAC                                                                                <  3:165240/149‑1                                                                   CTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGC‑GAAAGGGCCGCATAGCGGCCCCGGCAATTTCCGCGTGTATGCAACAAACCTGGGGCTGCTATGCAGCCCTTTCGCGACACAAG                                                                             <  4:25563/147‑1                                                                          CATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGC‑GAAAGGGCCGGATAGCGGCCCCGGCAATTTGCGCGTGTATGCAACAAACCTGGGGCTGCTATGCAGCCCTTTCGCGACACAAGGCCCACA                                                                      >  1:134158/1‑147                                                                          CATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTAGC‑GAAACGGCCGCATAGCGGCCCCGGCAATTTACGCGTGTATTCAACAAACCTGGGGCTGCTATGCAGCCCTTTAGCGCCACAAGGCCCACA                                                                      >  6:402607/1‑147                                                                              TGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGC‑GAAAGGGCCGCATAGCGGCCCCGGCAATTTCCGCGTGTATGCAACAAACCTGGGGCTGCTATGCAGCCCTTTCGCGACACAAGGCCCACAAAGGA                                                                 <  2:331572/148‑1                                                                                TCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGC‑GAAAGGGCCGCATAGCGGCCCCGGCAATTTCCGCGTGTATGCAACAAACCTGGGGGTGCTATGCAGCCCTTTCGGGACACAACGCCCACAAAGGACCG                                                              >  2:65595/1‑149                                                                                    CGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGC‑GAAAGGGCCGCATAGCGGCCCCGGCAATTTCCGCGTGTATGCAACAAACCTGGGGCTGCTATGCAGCCCTTTCGCGACACAAGGCCCACAAAGGACCGCGCA                                                          <  2:59545/149‑1                                                                                      ACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGC‑GAAAGGGCCGCATAGCGGCCCCGGCAATTTCCGCGTGTATGCAACAAACCT                                                                                                             >  1:12897/1‑96                                                                                      ACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGC‑GAAAGGGCCGCATAGCGGCCCCGGCAATTTCCGCGTGTATGCAACAAACCT                                                                                                             <  2:12897/96‑1                                                                                       CAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGC‑GAAAGGGCCGCATAGCGGCCCCGGCAATTTCCGCGTGTATGCAACAAACCTGGGGCTGCTATGCAGCCCTTTCGCGACACAAGGCCCACAAAGGACCGCGCAGGC                                                       <  1:390012/149‑1                                                                                          CATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGC‑GAAAGGGCCGCATAGCGGCCCCGGCAATTTCCGCGTGTATGCAACAAACCTGGGGCTGCTATGCAGCCCTTTCGCGACAGAAGGCCCACAAAGGACCGCGCAGGCTTC                                                    <  1:238656/149‑1                                                                                          CATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGC‑GAAAGGGCCGCATAGCGGCCCCGGCAATTTCCGCGTGTATGCAACAAACCTGGGGCTGCTATGCAGCCCTTTCGCGACACAAGGCCCACAAAGGACCGCGCAGGCTTC                                                    <  1:27785/149‑1                                                                                                AACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGC‑GAAAGGGCCGCATAGCGGCCCCGGCAATTTCCGCGTGTATGCAACAAACCTGGGGCTGCTATGCAGCCCTTTCGCGCCACAAGGCCCACAAAGGACCGCGCAGGCTTCAGGCCC                                              >  5:80115/1‑149                                                                                                AACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGC‑GAAAGGGCCGCATAGCGGCCCCGGCAATTTCCGCGTGTATGCAACAAACCTGGGGCTGCTATGCAGCCCTTTCGCGACACAAGGCCCACAAAGGACCGCGCAGGCTTCAGGCCC                                              <  1:65595/149‑1                                                                                                         CAGGTGTGGGAGCGGCCTTGTGTCGC‑GAAAGGGCCGCATAGCGGCCCCGGCAATTTCCGCGTGTATGCAACAAACCTGGGGCTGCTATGCAGCCCTTTCGCGACACAAGGCCCACAAAGGACCGCGCAAGCTTCAGGCCCGGAACTGGC                                     <  7:396515/149‑1                                                                                                            GTGTGGTAGCGGCCTTGTGTCGC‑GAAAGGGCCGCATAGCGGCCCCGTCAATTTCCGCGTGTATGCAACAAACCTGGGGCTGCTATGCAGCCCTTTCGCGACACAAGGCCCACAAAGGACCGCGCAGGCTTCAGGCCCGGAACTGGCCC                                   <  4:155950/148‑1                                                                                                                      GGCCTTGTGTCGC‑GAAAGGGCCGCATAGCGGCCCCGGCAATTTCCGCGTGTATGCAACAAACCTGGGGCTGCTATGCAGCCCTTTCGCGACACAAGGCCCACAAAGGACCGCGCAGGCTTCAGGCCCGGAACTGGCCCAGGCTGGCGCG                        >  2:71886/1‑149                                                                                                                        CCTTGTGTCGC‑GAAAGGGCCGCATAGCGGCCCCGGCAATTTCCGCGTGTATGCAACAAACCTGGGGCTGCTATGCAGCCCTTTCGCGACACAAGGCCCACAAAGGACCGCGCAGGCTTCAGGCCCGGAACTGGCCCAGGCTGGCGCG                        >  1:44109/1‑147                                                                                                                          TTGTGTCGC‑GAAAGGGCCGCATAGCGGCCCCGGCAATTTCCGCGTGTATGCAACAAACCTGGGGCTGCTATGCAGCCCTTTCGCGACACAAGGCCCACAAAGGACCGCGCAGGCTTCAGGCCCGGAACTGGCCCAGGCTGGCGCGCAGC                    >  6:206944/1‑149                                                                                                                            GTGTCGC‑GAAAGGGCCGCATAGCGGCCCCGGCAATTTCCGCGTGTATGCAACAAACCTGGGGCTGCTATGCAGCCCTTTCGCGACACAAGGCCCACAAAGGACCGCGCAGGCTTCAGGCCCGGAACTGGCCCAGGCTGGCGCGCAGC                    <  5:206944/147‑1                                                                                                                            GTGTCGC‑GAAAGGGCCGCATAGCGGCCCCGGCAATTTCCGCGTGTATGCAACAAACCTGGGGCTGCTATGCAGCCCTTTCGCGACACAAGGCCCACAAAGGACCGCGCAGGCTTCAGGCCCGGAACTGGCCCAGGCTGGCGCG                        <  6:326918/143‑1                                                                                                                            GTGTCGC‑GAAAGGGCCGCATAGCGGCCCCGGCAATTTCCGCGTGTATGCAACAAACCTGGGGCTGCTATGCAGCCCTTTCGCGACACAAGGCCCACAAAGGACCGCGCAGGCTTCAGGCCCGGAACTGGCCCAGGCTGGCGCG                        >  5:326918/1‑143                                                                                                                            GTGTCGC‑GAAAGGGCCGCATAGCGGCCCCGGCAATTTCCGCGTGTATGCAACAAACCTGGGGCTGCTATGCAGCCCTTTCGCGACACAAGGCCCAC                                                                       >  3:125499/1‑96                                                                                                                             tggCGC‑GAAAGGGCCGCATAGCGGCCCCGGCAATTTCCGCGTGTATGCAACAAACCTGGGGCTGCTATGCAGCCCTTTCGCGACACAAGGCCCACAAAGGACCGCGCAGGCTTCAGGCCCGGAACTGGCCCAGGCTGGCGCGCAGCTGA                 <  8:341941/146‑1                                                                                                                               TCGC‑GAAAGGGCCGCATAGCGGCCCCGGCAATTTCCGCGTGTATGCAACAAACCTGGGGCTGCTATGCAGCCCTTTCGCGACACAAGGCCCACAAAGGACCGCGCAGGCTTCAGGCCCGGAACTGGCCCAGGCTGGCGCGCAGCTGAGC               >  8:131783/1‑149                                                                                                                                 GC‑GAAAGGGCCGCATAGCGGCCCCGGCAATTTCCGCGTGTATGCAACAAACCTGGGGCTGCTATGCAGCCCTTTCGCGACACAAGGCCCACAAAGGACCGCGCAGGCTTCAGGCCCGGAACTGGCCCAGGCTGGCGCGCAGCTGAGCC              <  3:91980/148‑1                                                                                                                                  C‑GAAAGGGCCGCATAGCGGCCCCGGCAATTTCCGCGTGTATGCAACAAACCTGGGGCTGCTATGCAGCCCTTTCGCGACACAAGGCCCACAAAGGACCGCGCAGGCTTCAGGCCCGGAACTGGCCCAGGCTGGCGCGCAGCTGAGCCGC            >  1:207247/1‑149                                                                                                                                   tgccgGGGCCGCTATGCGGCCCCGGCAATTTCCGCGTGTATGCAACAAACCTGGGGCTGCTATGCAGCCCTTTCGCGACACAAGGCCCACAAAGGACCGCGCAGG                                                        >  1:238946/6‑105                                                                                                                                   tgccgGGGCCGCTATGCGGCCCCGGCAATTTCCGCGTGTATGCAACAAACCTGGGGCTGCTATGCAGCCCTTTCGCGACACAAGGCCCACAAAGGACCGCGCAGG                                                        <  2:238946/100‑1                                                                                                                                    GAAAGGGCCGCATAGCGGCCCCGGCAATTTCCGCGTGTATGCAACAAACCTGGGGCTGCTATGCAGCCCTTTCGCGACACAAGGCCCACAAAGGACCGCGCAGGCTTCAGGCCCGGAACTGGCCCAGGCTGGCGCGCAGCTGAGCCGCC           <  5:115296/149‑1                                                                                                                                    GAAAGGGCCGCATAGCGGCCCCGGCAATTTCCGCGTGTATGCAACAAACCTGGGGCTGCTATGCAGCCCTTTCGCGACACAAGGCCCACAAAGGACCGCGCAGGCTTCAGGCCCGGAACTGGCCCAGGCTGGCGCGCAGCTGAGCCGCC           <  7:214955/149‑1                                                                                                                                          GCCGCATAGCGGCCCCGGCAATTTCCGCGTGTATGCAACAAACCTGGGGCTGCTATGCAGCCCTTTCGCGACACAAGGCCCACAAAGGACCGCGCAGGCTTCAGGCCCGGAACTGGCCCAGGCTGGCGCGCAGCTGAGCCGCCAGATC      >  6:194320/1‑148                                                                                                                                             gcatccCGGCCCCGGCAATTTCCGCTTGTATGCAACAAACCTGGGGCTGCTATGCAGCCCTTTCGCGACACAAGGCCCACAAAGGACCGCGCAGGCTTCAGGCCCGGAACTGGCCCAGGCTGGCGCGCAGCTGAGCCGCCAGATCATCC  <  2:207247/143‑1                                                                                                                                             GCATAGCGGCCCCGGCAATTTCCGCGTGTATGCAACAAACCTGGGGCTGCTATGCAGCCCTTTCGCGACACAAGGCCCACAAAGGACCGCGCAGGCTTCAGGCCCGGAACTGGCCCAGGCTGGCGCGCAGCTGAGCCGCCAGATCATCC  <  1:71886/149‑1                                                                                                                                                     |||                                                                                                                                           GCTGTCACTGTGCAGGAAATTCAGGTCGGCCTGGGCAGCGATTAGTCGGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGC‑GAAAGGGCCGCATAGCG‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_002947/5555462‑5555608 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑CCGGCAATTTCCGCGTGTATGCAACAAACCTGGGGCTGCTATGCAGCCCTTTCGCGACACAAGGCCCACAAAGGACCGCGCAGGCTTCAGGCCCGGAACTGGCCCAGGCTGGCGCGCAGCTGAGCCGCCAGATCATCC  >  NC_002947/5555609‑5555746

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.