Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_002947	4,740,816	T→G	intergenic (+53/+700)	gltA → / ← yeiW	citrate synthase/putative oxidoreductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	4,740,816	0	T	G	100.0%	155.2 / NA	47	intergenic (+53/+700)	gltA/yeiW	citrate synthase/putative oxidoreductase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (25/22);  total (25/22)

TCCAGCCCGTACAAGATCGGCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGT‑AGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCCTTTTTTTATTTTTCTGGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGCGCATCCACCACGAACTTGTTGGCCACCA  >  NC_002947/4740677‑4740954                                                                                                                                             |                                                                                                                                           tcCAGCCCGTACAAGATCGGCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGT‑AGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCg                                                                                                                                  >  5:17486/1‑149 (MQ=255) tcCAGCCCGTACAAGATCGGCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGT‑AGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCg                                                                                                                                  >  4:115347/1‑149 (MQ=255)      cccGTACAAGATCGGCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGT‑AGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCctc                                                                                                                             <  3:115347/149‑1 (MQ=255)            cAAGATCGGCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGT‑AGACGAACGCAAGAACGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCCGCCTCATcgcg                                                                                                                       >  4:395148/1‑149 (MQ=255)                 tCGGCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGT‑AGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCtt                                                                                                                  <  3:86020/149‑1 (MQ=255)                       gTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGT‑AGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCcgc                                                                                                            <  6:17486/149‑1 (MQ=255)                          cgcgCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGT‑AGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGaa                                                                                                         <  2:94071/149‑1 (MQ=255)                           gcgcCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGT‑AGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCcgcg                                                                                                           >  6:119484/1‑146 (MQ=255)                           gcgcCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGT‑AGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAag                                                                                                        <  4:204409/149‑1 (MQ=255)                             gcCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGT‑AGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGGCAAGGCCGCGAagag                                                                                                      <  8:171364/149‑1 (MQ=38)                               cAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGT‑AGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGc                                                                                                    >  7:263049/1‑149 (MQ=255)                                        acCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGT‑AGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGc                                                                                                                                >  5:2797/1‑112 (MQ=255)                                        acCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGT‑AGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGc                                                                                                                                <  6:2797/112‑1 (MQ=255)                                                  aGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGT‑AGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACaaaa                                                                                 >  4:159963/1‑149 (MQ=255)                                                      gcgcGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGT‑AGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGGCAAGGCCGCGAaga                                                                                                       >  5:224044/1‑123 (MQ=37)                                                      gcgcGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGT‑AGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGGCAAGGCCGCGAaga                                                                                                       <  6:224044/123‑1 (MQ=37)                                                       cgcgACATCGTTGCCCTGAAACACCGCAAGTAAGCTGCAGT‑AGCCGAACGCAAGACGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCa                                                                            <  6:193795/149‑1 (MQ=255)                                                           aCATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGT‑AGCGGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTAGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGttt                                                                        >  3:294169/1‑149 (MQ=255)                                                            cATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGT‑AGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCGCAGtttt                                                                       >  5:397254/1‑149 (MQ=255)                                                                gTTGCCCTGAAAGACCGCAAGTAAGCTGCAGT‑AGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTtctc                                                                   >  6:216729/1‑149 (MQ=255)                                                                 ttGCCCTGAAAGACCGCACGTAAGCTCCTGT‑AGCCTACCGCAAGACGGCTGCCCTCGGGAAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCTAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTcc                                                                  <  6:397254/149‑1 (MQ=255)                                                                 ttGCCCTGAAAGACCGCAAGTAAGCTGCAGT‑AGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTcc                                                                  >  7:325111/1‑149 (MQ=255)                                                                    cccTGAAAGACCGCAAGTAAGCTGCAGT‑AGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTccgc                                                                <  5:119484/148‑1 (MQ=255)                                                                    cccTCAAACACCGCAATTAAGCTGCAGT‑ACCCGACCGCAAGACGGCTGCCCTCGGGCAGCGTTTTTTT‑TTTGTCCGGTCCCGGCCTCTGCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTccgcc                                                               <  4:37892/149‑1 (MQ=255)                                                                     ccTGAAAGACCGCAAGTAAGCTGCAGTGAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTATTAGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCACAGTTTTTCTacgcc                                                               >  1:235805/1‑149 (MQ=255)                                                                      cTGAAAGACCGCAAGTAAGCTGCAGT‑AGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCATCGCGGCCTTGCCCGCGAACAGGCCGGCACAGACACCCCAAAACCACAGTTTTTATccgcccg                                                             >  4:51056/1‑149 (MQ=255)                                                                       tGAAAGACCGCAAGTAAGCTGCAGT‑AGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTccgcccgc                                                            >  1:275125/1‑149 (MQ=255)                                                                           tgACCGCATGTAAGCTGCAGT‑AGCCTAACGCAATAAGGCTGCCCTGGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCAc                                                        <  4:294169/148‑1 (MQ=255)                                                                             aCCGCAAGTAAGCTGCAGT‑AGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACg                                                       >  4:280729/1‑148 (MQ=255)                                                                               cGCAAGTAAGCTGCAGT‑AGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTAGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCACAGTTTTTCTCCGCCCGCTCACg                                                       >  3:303926/1‑146 (MQ=255)                                                                                gCAAGTAAGCTGCAGT‑AGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGc                                                   <  8:325111/149‑1 (MQ=255)                                                                                  aaGTAAGCTGCAGT‑AGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTTCCCGCGAATAGGCCCGCACAGACACCACAAAACCTAAGttt                                                                        >  7:202070/1‑126 (MQ=255)                                                                                  aaGTAAGCTGCAGT‑AGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTAGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAATTTTTCTCCGCCCCCTCCCGCAcccc                                                 >  4:234653/1‑149 (MQ=255)                                                                                             aGT‑AGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGCCACCCCAAAACCTCAGTTTTTATACGCCCGCTCACGCAGccc                                                 >  5:330623/1‑138 (MQ=38)                                                                                              cttccccGAACGCAAGAAGCCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGATGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGccc                                                 <  6:330623/133‑1 (MQ=255)                                                                                                tagcgtaaCGCAAGAAGGGTGCGCGGGGGTAGCCTTTTTAT‑TTTGTCCTGTCCGGGCGTCTTCGCGGCCTTGCCCGCTATGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTAt                                      <  3:395148/141‑1 (MQ=255)                                                                                                         gCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGCGCa                           <  2:275125/149‑1 (MQ=255)                                                                                                           aagaagGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGCGCATc                         >  8:199168/1‑149 (MQ=255)                                                                                                              aagGCTGCCCGAGGGCAGCC‑TTCTTTATTTGTCCTGTGCCGGCCTCATCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCACTTTTTATCCGCCCCCTCCCGCAGCCCTTTAACGGGATTGAACGGCGCATccac                      >  2:232300/1‑149 (MQ=255)                                                                                                               agGCTGCCCTGGGGCTGCC‑TTTTTTATTTGTCCTGTGCCGGCCGCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGCGCATccacc                     <  3:51056/149‑1 (MQ=255)                                                                                                                ggCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGCGCATccacca                    <  2:117451/149‑1 (MQ=255)                                                                                                                     cccTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTGCGGGGCCTTCGCCGCGATGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGCGCATCCACCACGaa                <  4:116041/148‑1 (MQ=255)                                                                                                                       cTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTGTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGCAAGGCGCATCCACCACGAACtt             >  3:40665/1‑149 (MQ=255)                                                                                                                                gccttttttATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGCGCATCCACCACGAACTTGTTGGcca     <  7:199168/146‑1 (MQ=255)                                                                                                                                   ttttttATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGCGGCCGGCACAGACACCACAAAACCACATTTTTTAACCGCCCGATCACGCAGCCCTTTCAGGGTATTGAACGGCCCATCCCCCACGAACTTGTTGGccaca   >  6:210906/1‑147 (MQ=255)                                                                                                                                   ttttttATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCGCACGCAGCCCTTTAAGGGTATTTAACGGGGCATCCACCCCGAAATTGTTGGccacc   >  6:151039/1‑148 (MQ=255)                                                                                                                                    tttttATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCTAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGCGCATCCACCACGAACTTGTTGGccacca  <  3:234653/148‑1 (MQ=255)                                                                                                                                             |                                                                                                                                           TCCAGCCCGTACAAGATCGGCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGT‑AGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCCTTTTTTTATTTTTCTGGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGCGCATCCACCACGAACTTGTTGGCCACCA  >  NC_002947/4740677‑4740954

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑