| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_002947 | 1,499,508 | +C | intergenic (+85/‑79) | trpS → / → zapE | tryptophan‑‑tRNA ligase/nucleoside triphosphate hydrolase domain‑containing protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 1,499,506 | 1 | . | C | 97.6% | 114.3 / ‑3.1 | 41 | intergenic (+83/‑81) | trpS/zapE | tryptophan‑‑tRNA ligase/nucleoside triphosphate hydrolase domain‑containing protein |
| Reads supporting (aligned to +/- strand): ref base . (0/1); new base C (17/23); total (17/24) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.14e-01 | |||||||||||
AAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCCAC‑GGCTTTGCCGGTGT‑T‑TCGGGCA‑CG‑CCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTCCCGACTTCTTCCATGACGCGGCG > NC_002947/1499365‑1499649 | aaGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACC‑GGCTTTGCCGGTGT‑TCGCGGGCA‑cg‑cccgct < 7:250645/149‑5 (MQ=25) gCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACC‑GGCTTTGCCGGTGT‑TCGCGGGCA‑CGCCCGCTcc > 8:176930/1‑149 (MQ=25) gCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACC‑CGCGTTGCCGGTGT‑TAGCGGGCA‑CGCCCGCTcc > 6:114406/1‑149 (MQ=25) aTGCGGCATTTCAGAGCCTTAACGAAGCCTTGGCACCACAGCAGGACTGTGGCTGCCAACCGGTGGGAGCGGGGGTGCCCGCTAAACTGGCGCCGCGGTGCTTGGCACG‑GGCTTTGCGGGTGT‑TCGCGGGCA‑CGCCCGCTCCCACAGa < 4:276411/148‑1 (MQ=18) gCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACC‑GGCTTTGCCGGTGT‑TCGCGGGCA‑CGCCCGCTCCCACAGACTGAtt < 3:292115/149‑1 (MQ=21) ccATTCATAGCCTTAACGACGCCTTGGCACCACAGCAGCACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACCGGCGCCGCGGGGCATGGCACC‑GGCTTTGCCGGTGT‑TCGCGGGCA‑CGCCCGCTCCCACAGACTGATTg < 8:193668/149‑1 (MQ=18) cAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACC‑GGCTTTGCCGGTGT‑TCGCGGGCA‑CGCCCGCTCCCACAGACTGATTGCCAt < 8:261373/148‑1 (MQ=21) gCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACC‑GGCTTTGCCGGTGT‑TCGCGGGCA‑CGCCCGCTCCCACAGACTGATTGCCATTAAg < 8:113936/148‑1 (MQ=21) ccTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGGATGGCACC‑GGCTTTGCCGGTGT‑TCGCGGGCC‑CGCCCGCGCCCCCCGACTGATTGCCATTAAGCg > 3:105641/1‑149 (MQ=21) aaCGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGGGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACC‑GGCTTTGCCGGTGT‑TCGCGGGCA‑CGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGc < 5:114406/148‑1 (MQ=21) ggCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACC‑GGCTTTGCCGGTGT‑TAGCGGGCA‑CGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAg > 6:301317/1‑149 (MQ=25) accacAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACC‑GGCTTTGCCGGTGT‑TCGCGGGCA‑CGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGa < 5:377301/149‑1 (MQ=21) cagcagGACTGAGGCTGCCAAACGGTGGGAGGGGGCGTGCCCGCTAAACAGGCGCCGCGGTGCATGGCACG‑GGCTTTGCCGGTGT‑TCGCGGGCA‑CGCCCGCTCCCACAGACTGATTGCCATTAAGCg < 7:323708/127‑1 (MQ=21) aGGCTGCGAATCGGTGGGAGCGGGCGTGCCCGCTAAACAGGCGCCGCGTTGCATGGCACC‑GGCTTTGCCGGTGT‑TCGCGGGCA‑CGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGtttttt < 6:119911/147‑1 (MQ=21) aGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACC‑GGCTTTGCCGGTGT‑TCGCGGGCA‑CGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGtttttt < 5:301317/147‑1 (MQ=21) gCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACC‑GGCTTTGCCGGTGT‑TCGCGGGCA‑CGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCt < 1:9846/149‑1 (MQ=21) cAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACC‑GGCTTTGCCGGTGT‑TCGCGAGCA‑CGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGc > 7:115158/1‑112 (MQ=17) cAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACC‑GGCTTTGCCGGTGT‑TCGCGAGCA‑CGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGc < 8:115158/112‑1 (MQ=17) aaaCGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACC‑GGCTTTGCCGGTGT‑TCGCGGGCA‑CGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGc < 8:116249/111‑1 (MQ=17) cGTGCCCGCTAAACCGGCGCCGGGGGGCAT‑GCCACGGGCTTGGCGGGTGT‑TCGCGGGCA‑CGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTc < 6:242802/149‑1 (MQ=37) cGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACC‑GGCTTTGCCGGTGT‑TCGCGGGCA‑CGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTc < 8:121330/149‑1 (MQ=25) gCCCGCGAAACAGGCGCCGCGGTGCATGGCACC‑GGCTTTGCCGGTGT‑TCGCGGGCA‑CGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCt > 7:51662/1‑149 (MQ=37) cccGCGAAACAGGCGCCGCGGTGCATGGCACC‑GGCTTTGCCGGTGT‑TCGCGGGCA‑CGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCtt < 1:85324/149‑1 (MQ=37) ccGCGAAACAGGCGCCGCGGTGCATGGCACC‑GGCTTTGCCGGTGT‑TCGCGGGCA‑CGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCt > 2:209508/1‑147 (MQ=37) ccGCGAAACAGGCGCCGCGGTGCATGGCACC‑GGCTTTGCCGGTGT‑TCGCGGGCA‑CGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCt > 2:36904/1‑147 (MQ=37) ccGCGAAACAGGCGCCGCGGTGCATGGCACC‑GGCTTTGCCGGTGT‑TCGCGGGCA‑CGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCGCGCTAACGCATCATGACTCCCt > 4:227823/1‑147 (MQ=25) cgcgAAACAGGCGCCGCGGTGCATGGCACC‑GGCTTTGCCGGTGT‑TCGCGGGCA‑CGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCt > 3:27722/1‑146 (MQ=37) gcgAAACAGGCGCCGCGGTGCATGGCACC‑GGCTTTGCCGGTGT‑TCGCGGGCA‑CGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTATTGTTGCCTCGCTAACGAATCATGACTCCCTTAg > 7:102098/1‑148 (MQ=37) gAAACAGGCGCCGCGGGGCATGGCACC‑GGCTTTGCCGGTGTGC‑GCGGGCA‑CGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAAc < 5:213136/149‑1 (MQ=37) aaCAGGCGCCGCGGTGCATGGCACC‑GGCTTTGCCGGTGT‑TCGCGGGCA‑CGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGc > 6:80708/1‑149 (MQ=37) aGGCGCCGCGGTGCATGGCACC‑GGCTTTGCCGGTGT‑TCGCGGGCA‑CGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGGTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTAt > 5:17288/1‑149 (MQ=37) gcgcCGCGGTGCATGGCACC‑GGCTTTGCCGGTGT‑TCGCGGGCA‑CGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGAc > 7:210219/1‑95 (MQ=18) gcgcCGCGGTGCATGGCACC‑GGCTTTGCCGGTGT‑TCGCGGGCA‑CGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGAc < 8:210219/95‑1 (MQ=18) gcgcCGCGGTGCATGGCACC‑GGCTTTGCCGGTGT‑TCGCGGGCA‑CGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATc > 5:22878/1‑148 (MQ=37) cgccgcGGTGCATGGCACC‑GGCTTTGCCGGTGT‑TCGCGGGCA‑CGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCaa < 8:51662/149‑1 (MQ=37) ccgcGGTGCATGGCACC‑GGGTTTGCCGGTGT‑TCGCGGGCACCC‑CCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTGTCTAACGAATCATGACTCCCTTATAACGCTATCAAGc < 1:164392/149‑1 (MQ=37) ccGGTGT‑TCGCGGGCA‑CGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCt > 6:18122/1‑131 (MQ=255) ccGGTGT‑TCGCGGGCA‑CGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCt < 5:18122/131‑1 (MQ=255) gtgttcgcGGGCA‑CGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTCCCGACTTCTTCCa < 1:119380/142‑1 (MQ=255) gCA‑CGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGaa > 4:225005/1‑84 (MQ=255) gCA‑CGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGaa < 3:225005/84‑1 (MQ=255) gCA‑CGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTCCCGACTTCTTCCATGACgcggcg < 5:75052/149‑1 (MQ=255) | AAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCCAC‑GGCTTTGCCGGTGT‑T‑TCGGGCA‑CG‑CCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTCCCGACTTCTTCCATGACGCGGCG > NC_002947/1499365‑1499649 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |