Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,544,4940CG26.7% 47.9 / 15.6 30intergenic (+53/+61)mdfA/ampGmultidrug MFS transporter/muropeptide permease AmpG
Reads supporting (aligned to +/- strand):  ref base C (10/12);  new base G (4/4);  total (14/16)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

CGGGGGTGGCGGGGGCGTTGGCGCTGTGGCCGCTGTGGTTTGTGGTGGGTGGGCGGCCTGGGGAAGGGGCTGTAGCTCGATAATCGGTATTGCCGGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACACAGGGCCGCGCCGCTTTGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACAGGCAATACAAAGCTTCAGGGCCGCTCAGCCACCGAGTGGTCGT‑CCGCCACCGGCGTCCCCCGCCCGGCTTCCTGATGCCACTGCAGGGCGATCAG  >  NC_002947/1544359‑1544635
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gggggggggCGGGGCCGGTGGCGCTGTGGCCGCTGTGGTTTGTGGTGGGTGGGCGGCCTGGGGAAGGGGCTGTAGCTCGATAATCGGTATTGCCGGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACAAAGCGGCGCGGCCCTgtgt                                                                                                                                   <  5:162158/148‑1 (MQ=25)
 gggggTGGCGGGGGCGTTGGCGCTGTGGCCGCTGTGGTTTGTGGTGGGTGGGCGGCCTGGGGAAGGGGCTGTAGCTCGATAATCGGTATTGCCGGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACACAGGGCCGCGCCGCTTTg                                                                                                                                    >  6:199818/1‑147 (MQ=255)
 gggggTGGCGGGGGCGTTGGCGCTGTGGCCGCTGTGGTTTGTGGTGGGTGGGCGGCCTGGGGAAGGGGCTGTAGCTCGATAATCGGTATTGCCGGGGCCGGCCTCTTAGCGCGTAAAACCCCTCCTAAACCCGGCCCCCCCGCTTTGTa                                                                                                                                  >  6:295260/1‑149 (MQ=255)
        gCGGGGGCGTTGGCGCTGTGGCCGCTGTGGTTTGTGGTGGGTGGGCGGCCTGGGGAAGGGGCTGTAGCTCGATAATCGGTATTGCCGGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACAAAGCGGCGCGGCCCTGTGTAGGAGCgg                                                                                                                           <  6:82896/149‑1 (MQ=37)
         ggggggCGTGGCCGCGGGGGCCGCGGTGGTTTGTGTGGGGGGGGCGGCCTGGGGAAGGGGCTGTAGCTCGATAATCGGTATTGCCGGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACACAGGGCCGCGCCGCTTTGTAGGAGCggg                                                                                                                          <  8:81367/148‑1 (MQ=37)
                    gcgcTGTGGCCGCTGTGGTTTGTGGTGGGTGGGCGGCCTGGGGAAGGGGCTGTAGCTCGATAATCGGTATTGCCGGGGCCGGCCTCTTCGCGGGTTAAACCCCTCCGACCCACGGGCGCGCCGCGTTGTAGGAGCGGGGTTAACcccg                                                                                                                >  7:27635/1‑148 (MQ=255)
                        ggtgCCCGCGGGGGTTGGGGGGGGGGGGGCGGCCTGGGGAAGGGGCTGTAGCTCGATAATCGGTATTGCCGGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACACAGGGCCGCGCCGCTTTGTAGGAGCGGGTTTACCCGCGAagag                                                                                                           <  3:117624/148‑1 (MQ=25)
                            gCCGCTGTGGTTTGTGGTGGGTGGGCGGCCTGGGGAAGGGGCTGTAGCTCGATAATCGGTATTGCCGGGGCCGGCCTCATCGCGGGTAAACCCGCGCCAACACACGGCCCCGCCGCATTGTAAGAGCGGGGTTAACCGCGAAGAGGCCa                                                                                                       >  2:292734/1‑149 (MQ=255)
                                  ggggTTTGTGGGGGGGGGGCGGCCTGGGTACGTGGCTGTCGCGCGATAATCGGTATTGCCGGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACACAGGGCCGCGCCGCTTTGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACAg                                                                                                 <  3:257438/147‑1 (MQ=25)
                                                  gggCGGCCTGGGTATGGGGGTGTTGCTCGATATTGGTTTTTGCGGGGGCCGGCCTCTTGGCGGTTAAACCCGCTCCTACACTGGGCCGCGCGGGTTTTTAGGAGGGGGTTTACCCGCGAAGAGGCCAGCACAGGCAATACAAAGCTTCa                                                                                 <  8:27635/149‑1 (MQ=25)
                                                                        aagcgccaTAATCGGTATGCCCGGGGCCGGCCTCTTCGCGGGAAACCCCGCTCCAACCCCGGCCCGCGCCGCTTTGTAGGCGCGGGTTTACCCGCGAAGAGGCCAGCACAGGCAATACAAAGCTTCAGGGCCGCTCAGCCACCGAGTgg                                                           <  5:37790/142‑1 (MQ=37)
                                                                              gATAATCGGTATTGCCGGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACACAGGGCCGCGCCGCTTTGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACAGGCAATACAAAGCTTCAGGGCCGCTCAGCCACCGAGt                                                             <  7:208482/141‑1 (MQ=255)
                                                                                           gCCGGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACACAGGGCCGCGCCGCTTTGTAGGAGCGGTTTTACCCGCGAAGAGGCCAGCACAGGCAATACAAAGCTTCAGGGCCGCTCAGCCACCGAGTGGTCGT‑CCGCCACCGGCGTc                                        <  5:295260/148‑1 (MQ=255)
                                                                                                         ctgcgcgGGAAACCCCGCTCCAACACCGGCCCGCGCCGCTTTTTCGGCGCGGTTTAACCCGCGAAGAGGCCAGCACAGGCAATACAAAGCTTCAGGGCCGCTCAGCCACCGAGTGGTCGT‑CCGCCACCGGCGTCCCCCGCCCGGCTTcc                         <  1:161789/146‑1 (MQ=255)
                                                                                                          ttCGCGGGTAAACCCGCTCCTACACAGGGCCGCGCCGCTTTGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACAGGCAATACAAAGCTTCAGGGCCGCTCAGCCACCGAGTGGTCGT‑CCGCCACCGGCGTCCCCCGCCCGGCTTCCt                        >  7:308974/1‑149 (MQ=255)
                                                                                                          ttCGCGGGTAAACCCGCTCCTACACAGGGCCGCGCCGCTTTGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACAGGCAATACAAAGCTTCAGGGCCGCTCAGCCACCGAGTGGTCGT‑CCGCCACCGGCGTCCCCCGCCCGGCTTCCt                        >  3:39507/1‑149 (MQ=255)
                                                                                                            cgcgGGTAAACCCGCTCCTACACAGGGCCGCGCCGCTTTGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACAGGCAATACAAAGCTTCAGGGCCGCTCAGCCACCGAGTGGTCGT‑CCGCCACCGGCGTCCCCCGCCCGGCTTCCt                        <  5:199818/147‑1 (MQ=255)
                                                                                                             gcgGGTAAACCCGCTCCTACCCAGGGCCGCGCCGCTTTGTAGGAGCTGGTTTACCCGCGAAGAGGCCAGCACAGGCAATACAAAGCTTCAGGGCCGCTCAGCCACCGAGTGGTCGT‑CCGCCACCGGCGTCCCCCGCCCGGCTTCCTGAt                     <  8:308974/149‑1 (MQ=255)
                                                                                                               gggTAAACCCGCTCCTACACAGGGCCGCGCCGCTTTGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACAGGCAATACAAAGCTTCAGGGCCGCTCAGACACCGAGTGGTCGT‑CCGCCACCGGCGTCCCCCGCCCGGCTTCCTGATGc                   <  1:292734/149‑1 (MQ=255)
                                                                                                                 gTAAACCCGCTCCTACACAGGGCCGCGCCGCTTTGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACAGGCAATACAAAGCTTCAGGGCCGCTCAGCCACCGAGTGGTCGT‑CCGCCACCGGCGTCCCCCGCCCGGCTTCCTGATGCCa                 >  3:107198/1‑149 (MQ=255)
                                                                                                                      cccGCTCCTACACAGGGCCGCGCCGCTTTGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACAGGCAATACAAAGCTTCAGGGCCGCTCAGCCACCGAGTGGTCGT‑CCGCCACCGGCGTCCCCCGCCCGGCTTCCTGATGCCACTg              >  3:107884/1‑147 (MQ=255)
                                                                                                                        cgcgCCTACAACGGGGGGCGGCCCTGTGTTGTAGCGGGTTTACCCGCGAAGAGGCCGGCCCCGGCAATACCAAGCTTCAGGGCCGCTCAGCCACCGAGTGGTCGT‑CCGCCACCGGCGTCCCCCGCCCGGCTTCCTGATGCCACTGCAgg          <  5:181749/149‑1 (MQ=21)
                                                                                                                        cGCTCCTACACAGGGCCGCGCCGCTTTGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACAGGCAATACAAAGCTTCAGGGCCGCTCAGCCACCGAGTGGTCGT‑CCGCCACCGGCGTCCCCCGCCCGGCTTCCTGATGCCACTGCAgg          >  2:282266/1‑149 (MQ=255)
                                                                                                                        cGCTCCTACAAAGCGGCGCGGCCCTGTGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACAGGCAATACAAAGCTTCAGGGCCGCTCAGCCACCGAGTGGTCGT‑CCGCCACCGGCGTCCCCCGCCCGGCTTCCTGATGCCACTGCAgg          >  2:108945/1‑149 (MQ=37)
                                                                                                                        cGCTCATACAAAGCGGCGCGGCCCTGTGTAGGAGCGGGGTTACCCGCGAAGAGGCCAGCACAGGCAATCCAAAGCTTCAGGGCCGCTCAGCCCCCGAGGGGTCGT‑CCGCCCCCGGCGTCCCCCGCCCGGCTTCCTGATGCCACTGCAgg          >  5:274238/1‑149 (MQ=25)
                                                                                                                         gCTCCTACAAAGCGGCGCGGCCCTGTGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCACAGGCAATACAAAGCTTCAGGGCCGCTCAGCCACCGAGTGGTCGT‑CCGCCACCGGCGTCCCCCGCCCGGCTTCCTGATGCCACTGCAg           >  6:126185/1‑147 (MQ=25)
                                                                                                                         gCTCCTACAAAGCGGCGCGGCCCTGTGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCACAGGCAATACAAAGCTTCAGGGCCGCTCAGCCACCGAGTGGTCGT‑CCGCCACCGGCGTCCCCCGCCCGGCTTCCTGATGCCACTGCAg           <  5:126185/147‑1 (MQ=25)
                                                                                                                          cTCCTACACAGGGCCGCGCCGCTTTGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACAGGCAATACAAAGCTTCAGGGCCGCTCAGCCACCGAGTGGTCGTCCCGCCACCGGCGTCCCCCGCCCGGCTTCCTGATGCCACTGCAggg         <  1:282266/149‑1 (MQ=255)
                                                                                                                                 acaGGGCCGCGCCGCTTTGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACAGGCAATACAAAGCTTCAGGGCCGCTCAGCCACCGAGTGGTCGT‑CCGCCACCGGCGTCCCCCGCCCGGCTTCCTGATGCCACTGCAGGGCgatcag  >  6:264436/1‑148 (MQ=255)
                                                                                                                                 acaGGCCCGCGCCGCTTTGTAGGAGCGGGTTTACACGCGAAGAGGCCAGCACAGGCAATACAAATCTTCAGGGCCGCTCAGCCACCGAGTGGTCGT‑CCGCCACCGGCGTCCCCCGCCCGGCTTCCTGATGCAACTGCACGGCgataag  >  2:128099/1‑148 (MQ=39)
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CGGGGGTGGCGGGGGCGTTGGCGCTGTGGCCGCTGTGGTTTGTGGTGGGTGGGCGGCCTGGGGAAGGGGCTGTAGCTCGATAATCGGTATTGCCGGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACACAGGGCCGCGCCGCTTTGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACAGGCAATACAAAGCTTCAGGGCCGCTCAGCCACCGAGTGGTCGT‑CCGCCACCGGCGTCCCCCGCCCGGCTTCCTGATGCCACTGCAGGGCGATCAG  >  NC_002947/1544359‑1544635

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑