Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_002947	4,509,703	T→C	N120S (AAC→AGC)	serS ←	serine‑‑tRNA ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	4,509,703	0	T	C	100.0%	232.3 / NA	68	N120S (AAC→AGC)	serS	serine‑‑tRNA ligase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (35/33);  total (35/33)

GGACCACGCAGCACAGCAAAACGGGCACCGGACAGCTTGGCAGCCGCTTCGAAGTCCAGGCCACCGCTGATTTCACCGAGGGCCACATGGTCCTTGATTTCGAAGTCGAACGCCTTCGGCGTGCCCCAACGGCGCACTTCGACGTTGTCGTCTTCACTGGCGCCGACCGGCACGCTGGCGTCCGGC‑AGGTTGGGAATGGTCAGCAGG‑ATGCCATCCAGCTCGGCCTGAATGGCGTCCAGCTCAGCCTTGCCGGCAGCCAGTTCGTTGGCCATGCGCTCGACGTCAGCCA  >  NC_002947/4509559‑4509847                                                                                                                                                 |                                                                                                                                                   ggACCACGCAGCACAGCAAAACGGGCACCGGACAGCTTGGCAGCCGCTTCGAAGTCCAGGCCACCGCTGATTTCACCGAGGGCCACATGGTCCTTGATTTCGAAGTCGAACGCCTTCGGCGTGCCCCAACGGCGCACTTCGACGCTgtc                                                                                                                                                >  7:50717/1‑149 (MQ=255)  gACCACGCAGCACAGCAAAACGGGCACCGGACAGCTTGGCAGCCGCTTCGAAGTCCAGGCCACCGCTGATTTCACCGAGGGCCACATGGTCCTTGATTTCGAAGTCGAACGCCTTCGGCGTGCCCCAACGGCGCACTTCGACGCTgtcg                                                                                                                                               >  2:88087/1‑149 (MQ=255)            cacagcaAAACGGGCACCGGACAGCTTGGCAGCCGCTTCGAAGTCCAGGCCACCGCTGAATTCACCGAGGGCCACATGGTCCTTGATTTCGAAGACGAACGCCTTCTGCGTGCCCCAACGGCGCACATCGACGCTGTACTCTTCACTgg                                                                                                                                     >  7:364741/1‑149 (MQ=255)             acagcaAAACGGGCACCGGACAGCTTGGCAGCCGCTTCGAAGTCCAGGCCACCGCTGATTTCACCGAGGGCCACATGGTCCTTGATTTCGAAGTCGAACGCCTTCGGCGTGCCCCAACGGCGCACTTCGACGCTGTCGTCTTCACTGgc                                                                                                                                    >  5:253447/1‑149 (MQ=255)             acagcaAAACGGGCACCGGACAGCTTGGCAGCCGCTTCGAAGTCCAGGCCACCGCTGATTTCACCGAGGGCCACATGGTCCTTGATTTCGAAGTCGAACGCCTTCGGCGTGCCCCAACGGCGCACTTCGACGCTGTCGTCTTCACTGgc                                                                                                                                    <  7:148654/149‑1 (MQ=255)               agcaAAACGGCCACCGGACAGCTTGGCAGCCGCTTCGAAGACCAGCCCACCCCTGATTTCACCGAGGGCCACATGGTCCTTGATATCGAAGTCGAACGCCTTCGGCGTGCCCCAACGGCACACTTCGACGCTGTCGTCTTCACTTgccc                                                                                                                                  >  8:351107/1‑147 (MQ=255)                gcaAAACGGGCACCGGACAGCTTGGCAGCCGCTTCGAAGTCCAGGCCACCGCTGATTTCACCGAGCGCCACATGGTCCTTGATTTCGAAGTCGAACGCCTTCGGCGTGCCCCAACGGCGCACTTCGACGCTGTCGTCTTCACTGGCGcc                                                                                                                                 <  7:253149/149‑1 (MQ=255)                  aaaaCGGGCACCGGACAGCTTGGCAGCCGCTTCGAAGTCCAGGCCACCGCTGATTTCACCGAGGGCCACATGGTCCTTGATTTCGAAGTCGAACGCCTTCGGCGTGCCCCAACGGCGCACTTCGACGCTGTCGTCTTCACTGGCGCCGa                                                                                                                               <  1:94828/149‑1 (MQ=255)                   aaaCGGGCACCGGACAGCTTGGCAGCCGCTTCGAAGTCCAGGCCACCGCTGATTTCACCGAGGGCCACATGGTCCTTGATTTCGAAGTCGAACGCCTTCGGCGTGCCCCAACGGCGCACTTCGACGCTGTCGTCTTCACTGGCGCCGAc                                                                                                                              <  1:205065/149‑1 (MQ=255)                      cGGGCACCGGACAGCTTGGCAGCCGCTTCGAAGTCCAGGCCACCGCTGATTTCACCGAGGGCCACATGGTCCTTGATTTCGAAGTCGAACGCCTTCGGCGTGCCCCAACGGCGCACTTCGACGCTGTCGTCTTCACTGGCGCCGACCgg                                                                                                                           <  3:113999/149‑1 (MQ=255)                           atcGGACAGCTTGGCAGCCGCTTCGAAGTCCAGGCCACCGCTGATTTCTCCGATGGCCACATGGTCCTTGATTTCGAAGTCGAACGCCTTCGGCGTGCCCCAACGGCGCACTTCGACGCTGTCGTCTTCACTGGCGCCGACCGGCACGc                                                                                                                      <  8:364741/147‑1 (MQ=255)                             cGGACAGCTTGGCAGCCGCTTCGAAGTCCAGGCCACCGCTGATTTCGCCGAGGGCCACATGGTCCTTGATTTCGAAGTCGAACGCCTTCGGCGTGCCCCAACGGCGCACTTCGACGCTGTCGTCTTCACTGGCGCCGACCGGCACGCTg                                                                                                                    >  1:87306/1‑149 (MQ=255)                             cGGACAGCTTGGCAGCCGCTTCGAAGTCCAGGCCACCGCTGATTTCACCGAGGGCCACATGGTCCTTGATTTCGAAGTCGAACGCCTTCGGCGTGCCCCAACGGCGCACTTCGACGCTGTCGTCTTCACTGGCGCCGACCGGCACGCTg                                                                                                                    <  8:50717/149‑1 (MQ=255)                             cGGACAGCTTGGCAGCCGCTTCGAAGTCCAGGCCACCGCTGATTTCACCGAGGGCCACATGGTCCTTGATTTCGAAGTCGAACGCCTTCGGCGTGCCCCAACGGCGCACTTCGACGCTGTCGTCTTCACTGGCGCCGACCGGCACGCTg                                                                                                                    >  6:171273/1‑149 (MQ=255)                              ggACAGCTTGGCAGCCGCTTCGAAGTCCAGGCCACCGCTGATTTCACCGAGGGCCACATGGTCCTTGATTTCGAAGTCGAACGCCTTCGGCGTGCCCCAACGGCGCACTTCGACGCTGTCGTCTTCACTGGCGCCGACCGGCACGCTgg                                                                                                                   >  7:177448/1‑149 (MQ=255)                                    cTTGGCAGCCGCTTCGAAGTCCAGGCCACCGCTGATTTCACCGAGGGCCACATGGTCCTTGATTTCGAAGTCGAACGCCTTCGGCGTGCCCCAACGGCGCACTTCGACGCTGTCGTCTTCACTGGCGCCGACCGGCACGCTGGCGTCCg                                                                                                             >  6:366234/1‑149 (MQ=255)                                          aGCCGCTTCGAAGTCCAGGCCACCGCTGATTTCACCGAGGGCCACATGGTCCTTGATTTCGAACTCGAAAGCCTTCGGACTGCCCCAACGGCGCACTTCGACGCTGTCGTCTTCACTGGAGCCGACCGGCACGCTGGCGTCCGGC‑AGGt                                                                                                      >  7:253791/1‑149 (MQ=255)                                           gCCGCTTCGAAGTCCAGGCCACCGCTGATTTCACCGAGGGCCACATGGTCCTTGATTTCGAAGTCGAACGCCTTCGGCGTGCCCCAACGGCGCACTTCGACGCTGTCGTCTTCACTGGCGCCGACCGGCACGCTGGCGTCCGGC‑AGGtt                                                                                                     >  4:47528/1‑149 (MQ=255)                                               cTTCGAAGTCCAGGCCACCGCTGATTTCACCGAGGGCCACATGGTCCTTGATTTCGAAGTCGAACGCCTTCGGCGTGCCCCAACGGCGCACTTCGACGCTGTCGTCTTCACTGGCGCCGACCGGCACGCTGGCGTCCGGC‑AGGTTGGGa                                                                                                 <  1:268578/149‑1 (MQ=255)                                                   gAAGTCCAGGCCACCGCTGATTTCACCGAGGGCCACATGGTACCCAATTTCGAAGTCGAACGCCTTCGGCGTGCCCCAACGGCGCACTTCGACGCTGTCGTCTTCACTGGCGCCGACCGGCACGCTGGCGTCCGGC‑AGGTTGGGAATg                                                                                              <  1:132765/148‑1 (MQ=255)                                                    aaGTCCAGGCCACCGCTGATTTCACCGAGGGCCACATGGTCCTTGATTTCGAAGTCGAACGCCTTCGGCGTGCCCCAACGGCGCACTTCGACGCTGTCGTCTTCACTGGCGCCGACCGGCACGCTg                                                                                                                    >  2:90531/1‑126 (MQ=255)                                                    aaGTCCAGGCCACCGCTGATTTCACCGAGGGCCACATGGTCCTTGATTTCGAAGTCGAACGCCTTCGGCGTGCCCCAACGGCGCACTTCGACGCTGTCGTCTTCACTGGCGCCGACCGGCACGCTg                                                                                                                    <  1:90531/126‑1 (MQ=255)                                                      gTCCAGGCCACCGCTGATTTCACCGAGGGCCACATGGTCCTTGATTTCGAAGTCGAACGCCTTCGGCGTGCCACAACGGCGCACTTCGACGCTGTCGTCTTCACTGGCGCCGACCGGCACGCTGGCGTCCGGC‑AGGTTGGGAATGGTca                                                                                          <  7:163768/149‑1 (MQ=255)                                                         cAGGCCACCGCTGATTTCACCGAGGGCCACATGGTCCTTGATTTCGAAGTCGAACGCCTTCGGCGTGCCCCAACGGCGCACTTCGACGCTGTCGTCTTCACTGGCGCCGACCGGCACGCTGGCGTCCGGC‑AGGTTGGGAATGGTcag                                                                                         <  5:171273/147‑1 (MQ=255)                                                                ccGCTGATTTCACCGAGGGCCACATGGTCCTTGATTTCGAAGTCGAACGCCTTCGGCGTGCCCCAACGGCGCACTTCGACGCTGTCGTCTTCACTGGCGCCGACCGGCACGCTGGCGTCCGGC‑AGGTTGGGAATGGTCAGCAGG‑ATGcc                                                                               <  6:253447/149‑1 (MQ=255)                                                                 cGCTGATTTCACCGAGGGCCACATGGTCCTTGATTTCGAAGTCGAACGCCTTCGGCGTGCCCCAACGGCGCACTTCGACGCTGTCGTCTTCACTGGCGCCGACCGGCACGCTGGCGTCCGGC‑AGGTTGGGAATGGTCAGCAGG‑ATGCCa                                                                              >  5:340128/1‑149 (MQ=255)                                                                   cTGATTTCACCGAGGGCCACATGGTCCTTGATTTCGAAGTCGAACGCCTTCGGCGTGCCCCAACGGCGCACTTCGACGCTGTCGTCTTCACTGGCGCCGACCGGCACGCTGGCGTCCGGC‑AGGTTGGGAATGGTCAGCAGG‑ATGCCATc                                                                            >  8:274248/1‑149 (MQ=255)                                                                    tGATTTCACCGAGGGCCACATGGTCCTTGATTTCGAAGTCGAACGCCTTCGGCGTGCCCCAACGGCGCACTTCGACGCTGTCGTCTTCACTGGCGCCGACCGGCACGCTGGCGTCCGGC‑AGGTTGGGAATGGTCAGCAGG‑ATGCCAtt                                                                            >  8:9277/1‑147 (MQ=255)                                                                      aTTTCACCGAGGGCCACATGGTCCTTGATTTCGAAGTCGAACGCCTTCGGCGTGCCCCTACGGCGCACTTCGACGCTGTCGTCTTCACTGGCGCAGACCGGCACGCTGGCGTCCGGC‑AGGTTGGGAATGGTCAGCAGG‑ATGCCATCCa                                                                          <  7:351107/148‑1 (MQ=255)                                                                          cACCGAGGGCCACATGGTCCTTGATTTCGAAGTCGAACGCCTTCGGCGTGCCCCAACGGCGCACTTCGACGCTGTCGTCTTCACTGGCGCCGACCGGCACGCTGGCGTCCGGC‑AGGTTGGGAATGGTCAGCAGG‑ATGCCATCCAGCTCg                                                                     >  2:122928/1‑149 (MQ=255)                                                                           aCCGAGGGCCACATGGTCCTTGATTTCGAAGTCGAACGCCTTCGGCGTGCCCCAACGGCGCACTTCGACGCTGTCGTCTTCACTGGCGCCGACCGGCACGCTg                                                                                                                    >  6:352044/1‑103 (MQ=255)                                                                           aCCGAGGGCCACATGGTCCTTGATTTCGAAGTCGAACGCCTTCGGCGTGCCCCAACGGCGCACTTCGACGCTGTCGTCTTCACTGGCGCCGACCGGCACGCTg                                                                                                                    <  5:352044/103‑1 (MQ=255)                                                                           aCCGAGGGCCACATGGTCCTTGATTTCGAAGTCGAACGCCTTCGGCGTGCCCCAACGGCGCACTTCGACGCTGTCGTCTTCACTGGCGCCGACCGGCACGCTGGCGTCCGGC‑AGGTTGGGAATGGTCAGCAGG‑ATGCCATCCAGCTCgg                                                                    <  4:114248/149‑1 (MQ=255)                                                                           aCCGAGGGCCACATGGTCCTTGATTTCGAAGTCGAACGCCTTCGGCGTGCCCCAACGGCGCACTTCGACGCTGTCGTCTTCACTGGCGCCGACCGGCACGCTGGCGTCCGGC‑AGGTTGGGAATGGTCAGCAGG‑ATGCCATCCAGCTCgg                                                                    >  6:219676/1‑149 (MQ=255)                                                                            ccGAGGGCCACATGGTCCTTGATTTCGAAGTCGAACGCCTTCGGCGTGCCCCAACGGCGCACTTCGACGCTGTCGTCTTCACTGGCGCCGACCGGCACGCTGGCGTCCGGC‑AGGTTGGGAATGGTCAGCAGG‑ATGCCATCCAGCTCGGc                                                                   <  5:219676/149‑1 (MQ=255)                                                                             cGAGGGCCACATGGTCCTTGATTTCGAAGTCGAACGCCTTCGGCGTGCCCCAACGGCGCACTTCGACGCTGTCGTCTTCACTGGCGCCGACCGGCACGCTGGCGTCCGGC‑AGGTTGGGAATGGTCAGCAGG‑ATGCCATCCAGCTCGGcc                                                                  <  7:274248/149‑1 (MQ=255)                                                                                     acaTGGTCCTTGATTTCGAAGTCGAACGCCTTCGGCGTGCCCCAACGGCGCACTTCGACGCTGTCGTCTTCACTGGCGCCGACCGGCACGCTGGCGTCCGGC‑AGGTTGGGAATGGTCAGCAGG‑ATGCCATCCAGCTCGGCCTGAATg                                                            >  5:155243/1‑147 (MQ=255)                                                                                     acaTGGTCCTTGATTTCGAAGTCGAACGCCTTCGGCGTGCCCCAACGGCGCACTTCGACGCTGTCGTCTTCACTGGCGCCGACCGGCACGCTGGCGTCCGGC‑AGGTTGGGAATGGTCAGCAGG‑ATGCCATCCAGCTCGGCCTGAATg                                                            <  6:155243/147‑1 (MQ=255)                                                                                      caTGGTCCTTGATTTCGAAGTCGAACGCCTTCGGCGTGCCCCAACGGCGCACTTCGACGCTGTCGTCTTCACTGGCGCCGACCGGCACGCTGGCGTCCGGCAAGGTTGGGAATGGTCAGCAGG‑ATGCCATCCAGCTCGGCCTGAATGGc                                                          <  1:88087/149‑1 (MQ=255)                                                                                      caTGGTCCTTGATTTCGAAGTCGAACGCCTTCGGCGTGCCCCAACGGCGCACTTCGACGCTGTCGTCTTCACTGGCGCCGACCGGCACGCTGGCGTCCGGC‑AGGTTGGGAATGGTCAGCAGG‑ATGCCATCCAGCTCGGCCTGAATGGCg                                                         <  8:177448/149‑1 (MQ=255)                                                                                         ggTCCTTGATTTCGAAGTCGAACGCCTTCGGCGTGCCCCAACGGCGCACTTCGACGCTGTCGTCTTCACTGGCGCCGACCGGCACGCTGGCGTCCGGC‑AGGTTGGGAATGGTCAGCAGG‑ATGCCATCCAGCTCGGCCTGAATGGCGTc                                                       >  4:274809/1‑148 (MQ=255)                                                                                          gTCCTTGATTTCGAAGTCGAACGCCTTCGGCGTGCCCCAACGGCGCACTTCGACGCTGTCGTCTTCACTGGCGCCGACCGGCACGCTGGCGTCCGGC‑AGGTTGGGAATGGTCAGCAGG‑ATGCCATTCAGGCCGAGCTGGATGGCATcc                                                      <  7:9277/148‑1 (MQ=255)                                                                                          gTCCTTGATTTCGAAGTCGAACGCCTTCGGCGTGCCCCAACGGCGCACTTCGACGCTGTCGTCTTCACTGGCGCCGACCGGCACGCTGGCGTCCGGC‑AGGTTGGGAATGGTCAGCAGG‑ATGCCATCCAGCTCGGCCTGAATGGCGTcc                                                      <  6:340128/148‑1 (MQ=255)                                                                                           tCCTTGATTTCGAAGTCGAACGCCTTCGGCGTGCCCCAACGGCGCACTTCGACGCTGTCGTCTTCACTGGCGCCGACCGGCACGCTGGCGTCCGGC‑AGGTTGGGAATGGTCAGCAGG‑ATGCCATCCAGCTCGGCCTGAATg                                                            >  3:350344/1‑141 (MQ=255)                                                                                           tCCTTGATTTCGAAGTCGAACGCCTTCGGCGTGCCCCAACGGCGCACTTCGACGCTGTCGTCTTCACTGGCGCCGACCGGCACGCTGGCGTCCGGC‑AGGTTGGGAATGGTCAGCAGG‑ATGCCATCCAGCTCGGCCTGAATg                                                            <  4:350344/141‑1 (MQ=255)                                                                                             cTTGATTTCGAAGTCGAACGCCTTCGGCGTGCCCCAACGGCGCACTTCGACGCTGTCGTCTTCACTGGCGCCGACCGGCACGCTGGCGTCCGGC‑AGGTTGGGAATGGTCAGCAGG‑ATGCCATCCAGCTCGGCCTGAATGGCGTCCAGCt                                                  <  3:47528/149‑1 (MQ=255)                                                                                                 aTTTCGAAGTCGAACGCCTTCGGCGTGCCCCAACGGCGCACTTCGACGCTGTCGTCTTCACTGGCGCCGACCGGCACGCTGGCGTCCGGC‑AGGTTGGGAATGGTCAGCAGG‑ATGCCATCCAGCTCGGCCTGAATGGCGTCCAGCTCAGc                                              >  2:320128/1‑149 (MQ=255)                                                                                                 aTTTCGAAGTCGAACGCCTTCGGCGTGCCCCAACGGCGCACTTCGACGCTGTCGTCTTCACTGGCGCCGACCGGCACGCTGGCGTCCGGC‑AGGTTGGGAATGGTCAGCAGG‑ATGCCATCCAGCTCGGCCTGAATGGCGTCCAGCTCAGc                                              <  5:366234/149‑1 (MQ=255)                                                                                                     cGAAGTCGAACGCCTTCGGCGTGCCCCAACGGCGCACTTCGACGCTGTCGTCTTCACTGGCGCCGACCGGCACGCTGGCGTCCGGC‑AGGTTGGGAATGGTCAGCAGG‑ATGCCATCCAGCTCGGCCTGAATGGCGTCCAGCTCAGCCTTg                                          >  3:177903/1‑149 (MQ=255)                                                                                                          tCGAACGCCTTCGGCGTGCCCCAACGGCGCACTTCGACGCTGTCGTCTTCACTGGCGCCGACCGGCACGCTGGCGTCCGGC‑AGGTTGGGAATGGTCAGCAGGAATGCCATCCAGCTCGGCCTGAATGGCGTCCAGCTCAg                                               <  1:65261/140‑1 (MQ=255)                                                                                                          tCGAACGCCTTCGGCGTGCCCCAACGGCGCACTTCGACGCTGTCGTCTTCACTGGCGCCGACCGGCACGCTGGCGTCCGGC‑AGGTTGGGAATGGTCAGCAGG‑ATGCCATCCAGCTCGGCCTGAATGGCGTCCAGCTCAg                                               >  2:65261/1‑139 (MQ=255)                                                                                                                      ggCGTGCCCCAACGGCGCACTTCGACGCTGTCGTCTTCACTGGCGCCGACCGGCACGCTGGCGTCCGGC‑AGGTTGGGAATGGTCAGCAGG‑ATGCCATCCAGCTCGGCCTGAATGGCGTCCAGCTCAGCCTTGCCGGCAGCCAGTTCGtt                         <  1:320128/149‑1 (MQ=255)                                                                                                                      ggCGTGCCCCAACGGCGCACTTCGACGCGGTCGTCTTCACTGGCGCCGACCGGCACGGTGGCGTCCGGC‑AGGGTGGGAATGGTCACCAGG‑ATGCCATCCAGCTCGGCCTGAATGGCGTCCAGCTCAGCCTTGCCGGCAGCCAGTTCGtt                         <  6:222085/149‑1 (MQ=255)                                                                                                                       gCGTGCCCCAACGGCGCACTTCGACGCTGTCGTCTTCACTGGCGCCGACCGGCACGCTGGCGTCCGGC‑AGGTTGGGAATGGTCAGCAGG‑ATGCCATCCAGCTCGGCCTGAATGGCGTCCAGCTCAGCCTTGCCGGCAGCCAGTTCGTTg                        <  1:363232/149‑1 (MQ=255)                                                                                                                       gCGTGCCCCAACGGCGCACTTCGACGCTGTCGTCTTCACTGGCGCCGACCGGCACGCTGGCGTCCGGC‑AGGTTGGGAATGGTCAGCAGG‑ATGCCATCCAGCTCGGCCTGAATGGCGTCCAGCTCAGCCTTGCCGGCAGCCAGTTCGTTg                        >  3:357357/1‑149 (MQ=255)                                                                                                                             cccAACGGCGCACTTCGACGCTGTCGTCTTCACTGGCGCCGACCGGCACGCTGGCGTCCGGC‑AGGTTGGGAATGGTCAGCAGG‑ATGCCATCCAGCTCGGCCTGAATGGCGTCCAGCTCAGTCTTGCCGGCAGCCAGTTCGTTGGCCATg                  >  5:256686/1‑149 (MQ=255)                                                                                                                               cAACGGCGCACTTCGACGCTGTCGTCTTCACTGGCGCCGACCGGCACGCTGGCGTCCGGC‑AGGTTGGGAATGGTCAGCAGG‑ATGCCATCCAGCTCGGCCTGAATGGCGTCCAGCTCAGCCTTGCCGGCAGCCAGTTCGTTGGCCATgcg                >  8:75141/1‑149 (MQ=255)                                                                                                                                aaCGGCGCACTTCGACGCTGTCGTCTTCACTGGCGCCGACCGGCACGCTGGCGTCCGGC‑AGGTTGGGAATGGTCAGCAGG‑ATGCCATCCAGCTCGGCCTGaa                                                              <  8:99722/102‑1 (MQ=255)                                                                                                                                aaCGGCGCACTTCGACGCTGTCGTCTTCACTGGCGCCGACCGGCACGCTGGCGTCCGGC‑AGGTTGGGAATGGTCAGCAGG‑ATGCCATCCAGCTCGGCCTGaa                                                              >  7:99722/1‑102 (MQ=255)                                                                                                                                aaCGGCGCACTTCGACGCTGTCGTCTTCACTGGCGCCGACCGGCACGCTGGCGTCCGGC‑AGGTTGGGAATGGTCAGCAGG‑ATGCCATCCAGCTCGGCCTGAATGGCGTCCAGCTCAGCCTTGCCGGCAGCCAGTTCGTTGGCCATgcgc               >  3:154504/1‑149 (MQ=255)                                                                                                                                aaCGGCGCACTTCGACGCTGTCGTCTTCACTGGCGCCGACCGGCACGCTGGCGTCCGGC‑AGGTTGGGAATGGTCAGCAGG‑ATGCCATCCAGCTCGGCCTGAATGGCGTCCAGCTCAGCCTTGCCGGCAGCCAGTTCGTTGGCCATgcgc               >  4:128305/1‑149 (MQ=255)                                                                                                                                aaCGGCGCACTTCGACGCTGTCGTCTTCACTGGCGCCGACCGGCACGCTGGCGTCCGGC‑AGGTTGGGAATGGTCAGCAGG‑ATGCCATCCAGCTCGGCCTGAATGGCGTCCAGCTCAGCCTTGCCGGCAGCCAGTTCGTTGGCCATgcgc               <  4:177903/149‑1 (MQ=255)                                                                                                                                      gcACTTCGACGCTGTCGTCTTCACTGGCGCCGACCGGCACGCTGGCGTCCGGC‑AGGTTGGGAATGGTCAGCAGG‑ATGCCATCCAGCTCGGCCTGAATGGCGTCCAGCTCAGCCTTGCCGGCAGCCAGTTCGTTGGCCATGCGCTCGACg         >  2:19045/1‑149 (MQ=255)                                                                                                                                      gcACTTCGACGCTGTCGTCTTCACTGGCGCCGACCGGCACGCTGGCGTCCGGC‑AGGTTGGGAATGGTCAGCAGG‑ATGCCATCCAGCTCGGCCTGAATGGCGTCCAGCTCAGCCTTGCCGGCAGCCAGTTCGTTGGCCATGCGCTCGACg         >  3:166567/1‑149 (MQ=255)                                                                                                                                         cTTCGACGCTGTCGTCTTCACTGGCGCCGACCGGCACGCTGGCGTCCGGC‑AGGTTGGGAATGGTCAGCAGG‑ATGCCATCCAGCTCGGCCTGAATGGCGTCCAGCTCAGCCTTGCCGGCAGCCAGTTCGTTGGCCATGCGCTCGACGTCa      <  8:253791/149‑1 (MQ=255)                                                                                                                                           tCGACGCTGTCGTCTTCACTGGCGCCGACCGGCACGCTGGCGTCCGGC‑AGGTTGGGAATGGTCAGCAGG‑ATGCCATCCAGCTCGGCCTGAATGGCGTCCAGCTCAGCCTTGCCGGCAGCCAGTTCGTTGGCCATGCGCTCGACGTCAGc    >  8:108530/1‑149 (MQ=255)                                                                                                                                             gACGCTGTCGTCTTCACTGGCGCCGACCGGCACGCTGGCGTCCGGC‑AGGTTGGGAATGGTCAGCAGG‑ATGCCATCCAGCTCGGCCTGAATGGCGTCCAGCTCAGCCTTGCCGGCAGCCAGTTCGTTGGCCATGCGCTCGACGTCAGcc   >  6:368772/1‑148 (MQ=255)                                                                                                                                             gACGCTGTCGTCTTCACTGGCGCCGACCGGCACGCTGGCGTCCGGC‑AGGTTGGGAATGGTCAGCAGG‑ATGCCATCCAGCTCGGCCTGAATGGCGTCCAGCTCAGCCTTGCCGGCAGCCAGTTCGTTGGCCATGCGCTCGACGTCAGCCa  >  7:281167/1‑149 (MQ=255)                                                                                                                                                 |                                                                                                                                                   GGACCACGCAGCACAGCAAAACGGGCACCGGACAGCTTGGCAGCCGCTTCGAAGTCCAGGCCACCGCTGATTTCACCGAGGGCCACATGGTCCTTGATTTCGAAGTCGAACGCCTTCGGCGTGCCCCAACGGCGCACTTCGACGTTGTCGTCTTCACTGGCGCCGACCGGCACGCTGGCGTCCGGC‑AGGTTGGGAATGGTCAGCAGG‑ATGCCATCCAGCTCGGCCTGAATGGCGTCCAGCTCAGCCTTGCCGGCAGCCAGTTCGTTGGCCATGCGCTCGACGTCAGCCA  >  NC_002947/4509559‑4509847

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑