Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_002947 4,735,320 C→T M105I (ATG→ATA)  sucA ← 2‑oxoglutarate dehydrogenase subunit E1

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,735,3200CT98.2% 177.9 / NA 54M105I (ATG→ATA) sucA2‑oxoglutarate dehydrogenase subunit E1
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (26/27);  minor base A (1/0);  total (27/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CTCTTTGCCGATGAACAGGTCGCCGGCACGGAAGGTCGTATCAAGATCGGCATTGGTCAAGCCGTAGTGATTGATCGACAGGTCTACGGGCGCAGGGCGCTGCCACAACCCCAACGGGTCGAGCTTGGCAGCCTGATGGCCGCGCATACGATAGGCCTGGATCAGTCGCAGAACTTCAACCTGCTTCTTCTCGTGTTCACTGCTCACGCTCCCGGCGGAAACCGGTTGGGCGCGGCGCTGGTTCTTTGCCAGCAGTACGAAATGGTCGCGGATTGTCGAGTGCGATACA  >  NC_002947/4735176‑4735464
                                                                                                                                                |                                                                                                                                                
ctctTTGCCGATGAACAGGTCGCCGGCACGGAAGGTCGTATCAAGATCGGCATTGGTCAAGCCGTAGTGATTGATCGACGGGTCTACGGGCGCAGGGCGCTGCCACAACCCCAACGGGTCGAGCTTGGCAGCCTGATGGCCGCGTATAc                                                                                                                                              <  2:194718/149‑1 (MQ=255)
   tttGCCGATGAACAGGTCGCCGGCACGGAAGGTCGTATCAAGATCGGCATTGGTCAAGCCGAAGTGATTGATCGACAGGTCTACGGGCGCAGGGCGCTGCCACAACCCCAACGGGTCGAGCTTGGCAGCCTGATGGCCGCGTATACGa                                                                                                                                            >  4:191016/1‑148 (MQ=255)
                   tCGCCGGCACGGAAGGTCGTATCAAGATCGGCATTGGTCAAGCCGTAGTGATTGATCGACAGGTCTACGGGCGCAGGGCGCTGCCACAACCCCAACGGGTCGAGCTTGGCAGCCTGATGGCCGCGTATACGATAGGCCTGGATCAGTCg                                                                                                                           >  7:12362/1‑149 (MQ=255)
                       cGGCACGGAAGGTCGTATCAAGATCGGCATTGGTCAAGCCGTAGTGATTGATCGACAGGTCTACGGGCGCAGGGCGCTGCCACAACCCCAATGGGTCGAGCTTGGCAGCCTGATGGCCGCGTATACGATAGGCCTGGa                                                                                                                                  <  6:106505/138‑1 (MQ=255)
                       cGGCACGGAAGGTCGTATCAAGATCGGCATTGGTCAAGCCGTAGTGATTGATCGACAGGTCTACGGGCGCAGGGCGCTGCCACAACCCCAATGGGTCGAGCTTGGCAGCCTGATGGCCGCGTATACGATAGGCCTGGa                                                                                                                                  >  5:106505/1‑138 (MQ=255)
                         gCACGGAAGGTCGTATCAAGATCGGCATTGGTCAAGCCGTAGTGATTGATCGACAGGTCTACGGGCGCAGGGCGCTGCCACAACCCCAACGTGTCGAGCTTGGCAGCCTGATGGCCGCGTATACGATAGGCCTGGATCAGTCGCAGAAc                                                                                                                     <  6:20063/149‑1 (MQ=255)
                           aCGGAAGGTCGTATCAAGATCGGCATTGGTCAAGCCGTAGTGATTGATCGACAGGTCTACGGGCGCAGGGCGCTGCCACAACCCCAACGGGTCGAGCTTGGCAGCCTGATGGCCGCGTATACGATAGGCCTGGATCAGTCGCAGAACtt                                                                                                                   >  2:109318/1‑149 (MQ=255)
                            cGGAAGGTCGTATCAAGATCGGCATTGGTCAAGCCGTAGTGATTGATCGACAGGTCTACGGGCGCAGGGCGCTGCCACAACCCCAACGGGTCGAGCTTGGCAGCCTGATGGCCGCGTATACGATAGGCCTGGATCAGTCGCAGAACtt                                                                                                                   >  4:236181/1‑148 (MQ=255)
                                  gTCGTATCAAGATCGGCATTGGTCAAGCCGTAGTGATTGATCGACAGGTCTACGGGCGCAGGGCGCTGCCACAACCCCAACGGGTCGAGCTTGGCAGCCTGATGGCCGCGTATACGATAGGCCTg                                                                                                                                    >  8:142418/1‑125 (MQ=255)
                                  gTCGTATCAAGATCGGCATTGGTCAAGCCGTAGTGATTGATCGACAGGTCTACGGGCGCAGGGCGCTGCCACAACCCCAACGGGTCGAGCTTGGCAGCCTGATGGCCGCGTATACGATAGGCCTg                                                                                                                                    <  7:142418/125‑1 (MQ=255)
                                     gTATCAAGATCGGCATTGGTCAAGCCGTAGTGATTGATCGACAGGTCTACGGGCGCAGGGCGCTGCCACAACCCCAACGGGTCGAGCTTGGCAGCCTGATGGCCGCGTATACGATAGGCCTGGATCAGt                                                                                                                             <  8:87227/129‑1 (MQ=255)
                                     gTATCAAGATCGGCATTGGTCAAGCCGTAGTGATTGATCGACAGGTCTACGGGCGCAGGGCGCTGCCACAACCCCAACGGGTCGAGCTTGGCAGCCTGATGGCCGCGTATACGATAGGCCTGGATCAGt                                                                                                                             >  7:87227/1‑129 (MQ=255)
                                             aTCGGCATTGGTCAAGCCGTAGTGATTGATCGACAGGTCTACGGGCGCAGGGCGCTGCCACAACCCCAACGGGTCGAGCTTGGCAGCCTGATGGCCGCGTATACGATAGGCCTGGATCAGTCGCAGAACTTCAACCTGCTTCTTCTCgt                                                                                                 >  5:253773/1‑149 (MQ=255)
                                                  cATTGGTCAAGCCGTAGTGATTGATCGACAGGTCTACGGGCGCAGGGCGCTGCCACAACCCCAACGGGTCGAGCTTGGCAGCCTGATGGCCGCGTATACGATAGGCCTGGATCAGTCGCAGAACTTCAACCTGCTTCTTCTCGTGTTCa                                                                                            <  8:12362/149‑1 (MQ=255)
                                                               gTAGTGATTGATCGACAGGTCTACGGGCGCAGGGCGCTGCCACAACCCCAACGGGTCGAGCTTGGCAGCCTGATGGCCGCGTATACGATAGGCCTGGATCAGTCGCAGAACTTCAACCTGCTTCTTCTCGTGTTCACTGCTCACGCTcc                                                                               >  7:234472/1‑149 (MQ=255)
                                                                tAGTGATTGATCGACAGGTCTACGGGCGCAGGGCGCTGCCACAACCCCAACGGGTCGAGCTTGGCAGCCTGATGGCAGCGTATACGATAGGCCTGGATCAGTCGCAGAACTTCAACCTGCTTCTTCTCGTGTTCACTGCTCACGCTccc                                                                              >  1:301042/1‑149 (MQ=255)
                                                                   tgattgatCGACAGGTCTACGGGCGCAGGGCGCTGCCACAACCCCAACGGGTCGAGCTTGGCAGCCTGATGGCCGCGTATACGATAGGCCTGGATCAGTCGCAGAACTTCAACCTGCTTCTTCTCGTGTTCACTGCTCACGCTCCcggc                                                                           <  5:191666/149‑1 (MQ=255)
                                                                     attgatCGACAGGTCTACGGGCGCAGGGCGCTGCCACAACCCCAACGGGTCGAGCTTGGCAGCCTGATGGCCGCGTATACGATAGGCCTGGATCAGTCGCAGAACTTCAACCTGCTTCTTCTCGTGTTCACTGCTCACGCTCCcggcg                                                                          >  2:144662/1‑148 (MQ=255)
                                                                     attgatCGACAGGTCTACGGGCGCAGGGCGCTGCCACAACCCCAACGGGTCGAGCTTGGCAGCCTGATGGCCGCGTATACGATAGGCCTGGATCAGTCGCAGAACTTCAACCTGCTTCTTCTCGTGTTCACTGCTCACGCTCCcggcg                                                                          <  1:24534/148‑1 (MQ=255)
                                                                         atCGACAGGTCTACGGGCGCAGGGCGCTGCCACAACCCCAACGGGTCGAGCTTGGCAGCCTGATGGCCGCGTATACGATAGGCCTGGATCAGTCGCAGAACTTCAACCTGCTTCTTCTCGTGTTCACTGCTCACGCTCCCGGCGGAAAc                                                                     <  6:253773/149‑1 (MQ=255)
                                                                            gACAGGTCTACGGGCGCAGGGCGCTGCCACAACCCCAACGGGTCGAGCTTGGCAGCCTGATGGCCGCGTATACGATAGGCCTGGATCAGTCGCAGAACTTCAACCTGCTTCTTCTCGTGTTCACTGCTCACGCTCCCGGCGGAAACCgg                                                                  <  3:191016/149‑1 (MQ=255)
                                                                            gACAGGTCTACGGGCGCAGGGCGCTGCCACAACCCCAACGGGTCGAGCTTGGCAGCCTGATGGCCGCGTATACGATAGGCCTGGATCAGTCGCAGAACTTCAACCTGCTTCTTCTCGTGTTCACTGCTCACGCTCCCGGCGGAAACCg                                                                   <  4:286239/148‑1 (MQ=255)
                                                                             aCAGGTCTACGGGCGCAGGGCGCTGCCACAACCCCAACGGGTCGAGCTTGGCAGCCTGATGGCCGCGTATACGATAGGCCTGGATCAGTCGCAGAACTTCAACCTGCTTCTTCTCGTGTTCACTGCTCACGCTCCCGGCGGAAACCGGt                                                                 >  7:274756/1‑149 (MQ=255)
                                                                              cAGGTCTACGGGCGCAGGGCGCTGCCACAACCCCAACGGGTCGAGCTTGGCAGCCTGATGGCCGCGTATACGATAGGCCTGGATCAGTCGCAGAACTTCAACCTGCTTCTTCTCGTGTTCACTGCTCACGCTCCCGGCTGAAACCGGtt                                                                <  4:317949/149‑1 (MQ=255)
                                                                              cAGGTCTACGGGCGCAGGGCGCTGCCACAACCCCAACGGGTCGAGCTTGGCAGCCTGATGGCCGCGTATACGATAGGCCTGGATCAGTCGCAGAACTTCAACCTGCTTCTTCTCGTGTTCACTGCTCACGCTCCCGGCGGAAACCGGtt                                                                <  3:291595/149‑1 (MQ=255)
                                                                                  tCTACGGGCGCAGGGCGCTGCCACAACCCCAACGGGTCGAGCTTGGCAGCCTGATGGCCGCGTATACGATAGGCCTGGATCAGTCGCAGAACTTCAACCTGCTTCTTCTCGTGTTCACTGCTCACGCTCCCGGCGGAAACCGGTTGggc                                                            >  5:314892/1‑149 (MQ=255)
                                                                                        ggCGCAGGGCGCTGCCACAACCCCAACGGGTCGAGCTTGGCAGCCTGATGGCCGCGTATACGATAGGCCTGGATCAGTCGCAGAACTTCAACCTGCTTCTTCTCGTGTTCACTGCTCACGCTCCCGGCGGAAACCGGTTGggcgcggcg                                                      >  2:131734/1‑149 (MQ=255)
                                                                                         gcgcAGGGCGCTGCCACAACCCCAACGGGTCGAGCTTGGCAGCCTGATGGCCGCGTATACGATAGGCCTGGATCAGTCGCAGAACTTCAACCTGCTTCTTCTCGTGTTCACTGCTCACGCTCCCGGCGGAAACCGGTTGggcgcggcgc                                                     >  5:22165/1‑149 (MQ=255)
                                                                                           gcAGGGCGCTGCCACAACCCCAACGGGTCGAGCTTGGCAGCCTGATGGCCGCGTATACGATAGGCCTGGATCAGTCGCAGAACTTCAACCTGTTTCTTCTCGTGTTCACTGCTCACGCTCCCGGCGGAAACCGGTTGGGCGCGGCGCTg                                                   <  1:109318/149‑1 (MQ=255)
                                                                                             aGGGCGCTGCCACAACCCCAACGGGTCGAGCTTGGCAGCCTGATGGCCGCGTATACGATAGGCCTGGATCAGTCGCAGAACTTCAACCTGCTTCTtctc                                                                                                   <  7:230640/99‑1 (MQ=255)
                                                                                             aGGGCGCTGCCACAACCCCAACGGGTCGAGCTTGGCAGCCTGATGGCCGCGTATACGATAGGCCTGGATCAGTCGCAGAACTTCAACCTGCTTCTtctc                                                                                                   >  8:230640/1‑99 (MQ=255)
                                                                                               ggCGCTGCCACAACCCCAACGGGTCGAGCTTGGCAGCCTGATGGCCGCGTATACGATAGGCCTGGATCAGTCGCAGAACTTCAACCTGCTTCTTCTCGTGTTCACTGCTCACGCTCCCGGCGGAAACCGGTTGGGCGCGGCGCTGGTTc                                               >  6:3441/1‑149 (MQ=255)
                                                                                               ggCGCTGCCACAACCCCAACGGGTCGAGCTTGGCAGCCTGATGGCCGCGTATACGATAGGCCTGGATCAGTCGCAGAACTTCAACCTGCTTCTTCTCGTGTTCACTGCTCACGCTCCCGGCGGAAACCGGTTGGGCGCGGCGCTGGTTc                                               <  3:236181/149‑1 (MQ=255)
                                                                                                 cgcTGCCACAACCCCAACGGGTCGAGCTTGGCAGCCTGATGGCCGCGTAGACGATAGGCCTGGATCAGTCGCAGAACTTCAACCTGCTTCTTCTCGTGTTCACTGCTCCCGCTCCCGGCGGAAACCGGGTGGGCGCGGCGCTGGTTCtt                                             >  3:214958/1‑149 (MQ=255)
                                                                                                     gCCACAACCCCAACGGGTCGAGCTTGGCAGCCTGATGGCCGCGTATACGATAGGCCTGGATCAGTCGCAGAACTTCAACCTGCTTCTTCTCGTGTTCACTGCTCACGCTCCCGGCGGAAACCGGTTGGGCGCGGCGCTGGTTCTTTGc                                          >  6:201425/1‑148 (MQ=255)
                                                                                                       cacaACCCCAACGGGTCGAGCTTGGCAGCCTGATGGCCGCGTATACGATAGGCCTGGATCAGTCGCAGAACTTCAACCTGCTTCTTCTCGTGTTCACTGCTCACGCTCCCGGCGGAAACCGGTTGGGCGCGGCGCTGGTTCTTTGCcag                                       <  4:163338/149‑1 (MQ=255)
                                                                                                          aaCCCCAACGGGTCGAGCTTGGCAGCCTGATGGCCGCGTATACGATAGGCCTGGATCAGTCGCAGAACTTCAACCTGCTTCTTCTCGTGTTCACTGCTCACGCTCCCGGCGGAAACCGGTTGGGCGCGGCGCTGGTTCTTTGCcagcag                                    >  6:81364/1‑149 (MQ=255)
                                                                                                              ccAACGGGTCGAGCTTGGCAGCCTGATGGCCGCGAATACGATAGGCCTGGATCAGTCGCAGAACTTCAACCTGCTTCTTCTCGTGTTCACTGCTCACGCTCCCGGCGGAAACCGGTTGGGCGCGGCGCTGGTTCTTTGCCAGCAGTAc                                 >  1:58970/1‑148 (MQ=255)
                                                                                                               cAACGGGTCGAGCTTGGCAGCCTGATGGCCGCGTATACGATAGGCCTGGATCAGTCGCAGAACTTCAACCTGCTTCTTCTCGTGTTCACTGCTCACGCTCCCGGCGGAAACCGGTTGGGCGCGGCGCTGGTTCTTTGCCAGCAGTACg                                >  4:135272/1‑148 (MQ=255)
                                                                                                               cAACGGGTCGAGCTTGGCAGCCTGATGGCCGCGTATACGATAGGCCTGGATCAGTCGCAGAACTTCAACCTGCTTCTTCTCGTGTTCACTGCTCACGCTACCGGCGGAAACCGGTTGGGCGCGGCGCTGGTTCTTTGCCAGCAGTACg                                >  3:359878/1‑148 (MQ=255)
                                                                                                                      tCGAGCTTGGCAGCCTGATGGCCGCGTATACGATAGGCCTGGATCAGTCGCAGAACTTCAACCTGCTTCTTCTCGTGTTCACTGCTCACGCTCCCGGCGGAAACCGGTTGGGCGCGGCGCTGGTTCTTTGCCAGCAGTACGAAATGGTc                        <  8:274756/149‑1 (MQ=255)
                                                                                                                      tCGAGCTTGGCAGCCTGATGGCCGCGTATACGATAGGCCTGGATCAGTCGCAGAACTTCAACCTGCTTCTTCTCGTGTTCACTGCTCACGCTCCCGGCGGAAACCGGTTGGGCGCGGCGCTGGTTCTTTGCCAGCAGTACGAAATGGTc                        >  4:159374/1‑149 (MQ=255)
                                                                                                                      tCGAGCTTGGCAGCCTGATGGCCGCGTATACGATAGGCCTGGATCAGTCGCAGAACTTCAACCTGCTTCTTCTCGTGTTCACTGCTCACGCTCCCGGCGGAAACCGGTTGGGCGCGGCGCTGGTTCTTTGCCAGCAGTACGAAATGGTc                        <  8:234472/149‑1 (MQ=255)
                                                                                                                        gAGCTTGGCAGCCTGATGGCCGCGTATACGATAGGCCTGGATCAGTCGCAGAACTTCAACCTGCTTCTTCTCGTGTTCACTGCTCACGCTCCCGGCGGAAACCGGTTGGGCGCGGCGCTGGTTCTTTGCCAGCAGTACGAAATGGTcgc                      <  4:61285/149‑1 (MQ=255)
                                                                                                                          gCTTGGCAGCCTGATGGCCGCGTATACGATAGGCCTGGATCAGTCGCAGAACTTCAACCTGCTTCTTCTCGTGTTCACTGCTCACGCTCCCGGCGGAAACCGGTTGGGCGCGGCGCTGGTTCTTTGCCAGCAGTACGAAATGGTCGCgg                    <  2:301042/149‑1 (MQ=255)
                                                                                                                              ggCAGCCTGATGGCCGCGTATACGATAGGCCTGGATCAGTCGCAGAACTTCAACCTGCTTCTTCTCGTGTTCACTGCTCACGCTACCGGCGGAAACCGGTTGGGCGCGGCGCTGGTTCTTTGCCAGCAGTACGAAATGGTCGCGGAtt                 <  4:359878/148‑1 (MQ=255)
                                                                                                                                cAGCCTGATGGCCGCGTATACGATAGGCCTGGATCAGTCGCAGAACTTCAACCTGCTTCTTCTCGTGTTCACTGCTCACGCTCCCGGCGGAAACCGGTTGGGCGCGGCGCTGGTTCTTTGCCAGCAGTACGAAATGGTCGCGGATTGTc              <  5:81364/149‑1 (MQ=255)
                                                                                                                                   ccTGATGGCCGCGTATACGATAGGCCTGGATCAGTCGCAGAACTTCAACCTGCTTCTTCTCGTGTTCACTGCTCACGCTCCCGGCGGAAACCGGTTGGGCGCGGCGCTGGTTCTTTGcc                                         <  3:18655/119‑1 (MQ=255)
                                                                                                                                   ccTGATGGCCGCGTATACGATAGGCCTGGATCAGTCGCAGAACTTCAACCTGCTTCTTCTCGTGTTCACTGCTCACGCTCCCGGCGGAAACCGGTTGGGCGCGGCGCTGGTTCTTTGcc                                         >  4:18655/1‑119 (MQ=255)
                                                                                                                                       aTGGCCGCGTATACGATAGGCCTGGATCAGTCGCAGAACTTCAACCTGCTTCTTCTCGTGTTCACTGCTCACGCTCCCGGCGGAAACCGGTTGGGCGCGGCGCTGGTTCTTTGCCAGCAGTACGAAATGGTCGCGGATTGTCGAGTg         <  7:69324/147‑1 (MQ=255)
                                                                                                                                          gCCGCGTATACGATAGGCCTGGATCAGTCGCAGAACTTCAACCTGCTTCTTCTCGTGTTCACTGCTCACGCTCCCGGCGGAAACCGGTTGGGCGCGGCGCTGGTTCTTTGCCAGCAGTACGAAATGGTCGCGGATTGTCGAGTGCGAt     <  6:314892/148‑1 (MQ=255)
                                                                                                                                           ccGCGTATACGATAGGCCTGGATCAGTCGCAGAACTTCAACCTGCTTCTTCTCGTGTTCACTGCTCACGCTCCCGGCGGAAACCGGTTGGGCGCGGCGCTGGTTCTTTGCCAGCAGTACGAAATGGTCGCGGATTGTCGAGTGCGATa    >  8:73541/1‑148 (MQ=255)
                                                                                                                                           ccGCGTATACGATAGGCCTGGATCAGTCGCAGAACTTCAACCTGCTTCTTCTCGTGTTCACTGCTCACGCTCCCGGCGGAAACCGGTTGGGCGCGGCGCTGGTTCTTTGCCAGCAGTACGAAATGGTCGCGGATTGTCGAGTGCGATa    >  1:104568/1‑148 (MQ=255)
                                                                                                                                            cgcgTATACGATAGGCCTGGATCAGTCGCAGAACTTCAACCTGCTTCTTCTCGTGTTCACTGCTCACGCTCCCGGCGGAAACCGGTTGGGCGCGGCGCTGGTTCTTTGCCAGCAGTACGAAATGGTCGCGGATTGTCGAGTGCGATACa  <  1:144662/149‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                                
CTCTTTGCCGATGAACAGGTCGCCGGCACGGAAGGTCGTATCAAGATCGGCATTGGTCAAGCCGTAGTGATTGATCGACAGGTCTACGGGCGCAGGGCGCTGCCACAACCCCAACGGGTCGAGCTTGGCAGCCTGATGGCCGCGCATACGATAGGCCTGGATCAGTCGCAGAACTTCAACCTGCTTCTTCTCGTGTTCACTGCTCACGCTCCCGGCGGAAACCGGTTGGGCGCGGCGCTGGTTCTTTGCCAGCAGTACGAAATGGTCGCGGATTGTCGAGTGCGATACA  >  NC_002947/4735176‑4735464

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑