Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,186,049 Δ1 bp 6.6% coding (411/1716 nt) aceK ← isocitrate dehydrogenase kinase/phosphatase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,186,0490G. 6.6% 194.0 / 7.1 61coding (411/1716 nt)aceKisocitrate dehydrogenase kinase/phosphatase
Reads supporting (aligned to +/- strand):  ref base G (27/30);  new base . (2/2);  total (29/32)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.96e-01

GGCAGGCATTCGCGCAGTTGTTCTTCCAGCCGCGCCAGGTCGCCTTCCAGGTCGCCATAGGGCATGTCGAACGGGTAATCGGCGAACACCGCGCGCAGCAAGTTCCTGAGACTGCCGTCAAGCCGATAGGTACGGGTTTGCGCGGCGCGCTCGCG‑GCCGCGCATGGAAGGCCGGGTAGTGTGGATGAACATGCAGCCGTCGCTGATCAGGTCATGGCTGAAGAGGCTGCAAAACAGCGAGTTGTACCAGGTCTCTGCCAACTCGTCGTCCAGGCGGGGGTCGATCA  >  NC_002947/5185903‑5186188
                                                                                                                                                  |                                                                                                                                            
ggcaggcaTTCGCGCAGTTGTTCTTCCAGCCGCGCCAGGTCGCCTTCCAGGTCGCCATAGGGCATGTCGAACGGGTAATCGGCGAACACCGCGCGCAGCAAGTTCCTGAGACTGCCGTCAAGCCGATAGGTACGGGTTTgcgcggcgcg                                                                                                                                            >  3:262659/1‑149 (MQ=255)
   aggcaTTCGCGCAGTTGTTCTTCCAGCCGCGCCAGGTCGCCTTCCAGGTCGCCATAGGGCATGTCGAACGGGTAATCGGCGAACACCGCGCGCAGCAAGTTCCTGAGACTGCCGTCAAGCCGATAGGTACGGGTTTGCGCGGCGCGCTc                                                                                                                                         >  6:166334/1‑149 (MQ=255)
   aggcaTTCGCGCAGTTGTTCTTCAAGCCGCGCCAGGTCGCCTTCCAGGTCGCCATAGTGCATGTCGAACGGGTACTCGGCGACCACCGCGCGCAGCAAGTTCCTGAGACTGCCGTCAAGCCTATAGGTACGGGTTTGCGCGGCGCGCTc                                                                                                                                         <  1:72941/149‑1 (MQ=255)
     gcaTTCGCGCAGTTGTTCTTCCAGCCGCGCCAGGTCGCCTTCCAGGTCGCCATAGGGCATGTCGAACGGGTAATCGGCGAACACCGCGCGCAGCAAGTTCCTGAGACTGCCGTCAAGCCGATAGGTACGGGTTTGCGCGGCGCGCTcgc                                                                                                                                       >  2:183714/1‑149 (MQ=255)
                       ttcCAGCCGCGCCAGGTCGCCTTCCAGGTCGCCATAGGGCATGTCGAACGGGTAATCGGCGAACACCGCGCGCAGCAAGTTCCTGAGACTGCCGTCAAGCCGATAGGTACGGGTTTGCGCGGCGCGCTCGCG‑GCCGCGCATGGAAGGcc                                                                                                                    >  8:261123/1‑149 (MQ=255)
                       ttcCAGCCGCGCCAGGTCGCCTTCCAGGTCGCCATAGGGCATGTCGAACGGGTAATCGGCGAACACCGCGCGCAGCAAGTTCCTGAGACTGCCGTCAAGCCGATAGGTACGGGTTTGCGCGGC‑CGCGAGCGCGCCGCGCaaacccgt                                                                                                                      >  6:101995/1‑140 (MQ=255)
                       ttcCAGCCGCGCCAGGTCGCCTTCCAGGTCGCCATAGGGCATGTCGAACGGGTAATCGGCGAACACCGCGCGCAGCAAGTTCCTGAGACTGCCGTCAAGCCGATAGGTACGGGTTTGCGCGGC‑CGCGAGCGCGCCGCGCaaacccgt                                                                                                                      <  5:101995/147‑8 (MQ=255)
                       ttcCAGCCGCGCCAGGTCGCCTTCCAGGTCGCCATAGGGCATGTCGAACGGGTAATCGGCGAACACCGCGCGCAGCAAGTTCCTGAGACTGCCGTCAAGCCGATAGGTACGGGTTTGCGCGGC‑CGCGAGCGCGCCGCGCaaaccc                                                                                                                        <  4:136543/145‑6 (MQ=255)
                       ttcCAGCCGCGCCAGGTCGCCTTCCAGGTCGCCATAGGGCATGTCGAACGGGTAATCGGCGAACACCGCGCGCAGCAAGTTCCTGAGACTGCCGTCAAGCCGATAGGTACGGGTTTGCGCGGC‑CGCGAGCGCGCCGCGCaaaccc                                                                                                                        >  3:136543/1‑140 (MQ=255)
                         ccAGCCGCGCCAGGTCGCCTTCCAGGTCGCCATAGGGCATGTCGAACGGGTAATCGGCGAACACCGCGCGCAGCAAGTTCCTGAGACTGCCGTCAAGCCGATAGGTACGGGTTTGCGCGGCGCGCTCGCG‑GCCGCGCATGGGAGGc                                                                                                                     <  6:241880/146‑1 (MQ=255)
                          cAGCCGCGCCAGGTCGCCTTCCAGGTCGCCATAGGGCATGTCGAACGGGTAATCGGCGAACACCGCGCGCAGCAAGTTCCTGAGACTGCCGTCAAGCCGATAGGTACGGGTTTGCGCGGCGCGCTCGCG‑GCCGCGCATGGAAGGCCggg                                                                                                                 >  6:141928/1‑149 (MQ=255)
                           aGCCGCGCCAGGTCGCCTTCCAGGTCGCCATAGGGCATGTCGAACGGGTAATCGGCGAACACCGCGCGCAGCAAGTTCCTGAGACTGCCGTCAAGCCGATAGGTACGGGTTTGCGCGGCGCGCTCGCG‑GCCGCGCATGGAAGGCCggg                                                                                                                 >  4:234982/1‑148 (MQ=255)
                           aGCCGCGCCAGGTCGCCTTCCAGGTCGCCATAGGGCATGTCGAACGGGTAATCGGCGAACACCGCGCGCAGCAAGTTCCTGAGACTGCCGTCAAGCCGATAGGTACGGGTTTGCGCGGCGCGCTCGCG‑GCCGCGCATGGAAGGCCGGGt                                                                                                                >  3:90254/1‑149 (MQ=255)
                               gcgcCAGGTCGCCTTCCAGGTCGCCATAGGGCATGTCGAACGGGTAATCGGCGAACACCGCGCGCAGCAAGTTCCTGAGACTTCCGTCAAGCCGATAGGTACGGGTTTGCGCGGCGCGCTCGCG‑GCCGCGCATGGAAGGCCGGGTAgtg                                                                                                            >  2:251865/1‑149 (MQ=255)
                               gcgcCAGGTCGCCTTCCAGGTCGCCATAGGGCATGTCGAACGGGTAATCGGCGAACACCGCGCGCAGCAAGTTCCTGAGACTGCCGTCAAGCCGATAGGTACGGGTTTGCGCGGCGCGCTCGCG‑GCCGCGCATGGAAGGCCGGGTAgtg                                                                                                            >  4:301340/1‑149 (MQ=255)
                                cgcCAGGTCGCCTTCCAGGTCGCCATAGGGCATGTCGAACGGGTAATCGGCGAACACCGCGCGCAGCAAGTTCCTGAGACTGCCGTCAAGCCGATAGGTACGGGTTTGCGCGGCGCGCTCGCG‑GCCGCGCATGGAAGGCCGGGTAgtgt                                                                                                           <  7:148663/149‑1 (MQ=255)
                                   cAGGTCGCCTTCCAGGTCGCCATAGGGCATGTCGAACGGGTAATCGGCGAACACCGCGCGCAGCAAGTTCCTGAGACTGCCGTCAAGCCGATAGGTACGGGTTTGCGCGGCGCGCTCGCG‑GCCGCGCATGGAAGGCCGGGTAGTGTGGa                                                                                                        <  3:275182/149‑1 (MQ=255)
                                   cAGGTCGCCTTCCAGGTCGCCATAGGGCATGTCGAACGGGTAATCGGCGAACACCGCGCGCAGCAAGTTCCTGAGACTGCCGTCAAGCCGATAGGTACGGGTTTGCGCGGCGCGCTCGCG‑GCCGCGCATGGAAGGCCGGGTAGTGTGGa                                                                                                        <  4:90254/149‑1 (MQ=255)
                                     ggTCGCCTTCCAGGTCGCCATAGGGCATGTCGAACGGGTAATCGGCGAACACCGCGCGCAGCAAGTTCCTGAGACTGCCGTCAAGCCGATAGGTACGGGTTTGCGCGGCGCGCTCGCG‑GCCGCGCATGGAAGGCCGGGTAGTGTGGATg                                                                                                      <  6:36868/149‑1 (MQ=255)
                                      gTCGCCTTCCAGGTCGCCATAGGGCATGTCGAACGGGTAATCGGCGAACACCGCGCGCAGCAAGTTCCTGAGACTGCCGTCAAGCCGATAGGTACGGGTTTGCGCGGCGCGCTCGCG‑GCCGCGCATGGAAGGCCGGGTAGTGTGGATg                                                                                                      >  3:359396/1‑148 (MQ=255)
                                       tCGCCTTCCAGGTCGCCATAGGGCATGTCGAACGGGTAATCGGCGAACACCGCGCGCAGCAAGTTCCTGAGACTGCCGTCAAGCCGATAGGTACGGGTTTGCGCGGCGCGCTCGCG‑GCCGCGCATGGAAGGCCGGGTAGTGTGGATGaa                                                                                                    >  6:163940/1‑149 (MQ=255)
                                       tCGCCTTCCAGGTCGCCATAGGGCATGTCGAACGGGTAATCGGCGAACACCGCGCGCAGCAAGTTCCTGAGACTGCCGTCAAGCCGATAGGTACGGGTTTGCGCGGCGCGCTCGCG‑GCCGCGCATGGAAGGCCGGGTAGTGTGGATGa                                                                                                     >  7:301258/1‑148 (MQ=255)
                                       tCGCCTTCCAGGTCGCCATAGGGCATGTCGAACGGGTAATCGGCGAACACCGCGCGCAGCAAGTTCCTGAGACTGCCGTCAAGCCGATAGGTACGGGTTTGCGCGGCGCGCTCGCG‑GCCGCGCATGGAAGGCCGGGTAGTGTGGATGa                                                                                                     <  8:301258/148‑1 (MQ=255)
                                        cGCCTTCCAGGTCGCGATAGTGCATGTCGAACGGGTAATCGGCGAAGACCGCGCGCAGCAAGTTCCTGAGACTGCCGTCAAGCCGATAGGTACGGGTTTGCGCGGCGCGCTCGCG‑GCCGCGCATGGATGGCGGGGTAGTGTGGATGAAc                                                                                                   <  8:72682/149‑1 (MQ=255)
                                           cTTCCAGGTCGCCATAGGGCATGTCGAACGGGTAATCGGCGAACACCGCGCGCAGCAAGTTCCTGAGACTGCCGTCAAGCCGATAGGTACGGGTTTGCGCGGCGCGCTCGCG‑GCCGCGCATGGAAGGCCGGGTAGTGTGGATGAACATg                                                                                                <  7:192823/149‑1 (MQ=255)
                                             tCCAGGTCGCCATAGGGCATGTCGAACGGGTAATCGGCGAACACCGCGCGCAGCAAGTTCCTGAGACTGCCGTCAAGCCGATAGGTACGGGTTTGCGCGGCGCGCTCGCG‑GCCGCGCATGGAAGGCCGGGAAGTGTGGATGAACATg                                                                                                >  7:258918/1‑147 (MQ=255)
                                               cAGGTCGCCATAGGGCATGTCGAACGGGTAATCGGCGAACACCGCGCGCAGCAAGTTCCTGAGACTGCCGTCAAGCCGATAGGTACGGGTTTGCGCGGCGCGCTCGCG‑GCCGCGCATGGAAGGCCGGGTAGTTTGGATGAACATGCAGc                                                                                            >  3:50159/1‑149 (MQ=255)
                                               cAGGTCGCCATAGGGCATGTCGAACGGGTAATCGGCGAACACCGCGCGCAGCAAGTTCCTGAGACTGCCGTCAAGCCGATAGGTACGGGTTTGCGCGGCGCGCTCGCG‑GCCGCGCATGGAAGGCCGGGTAGTGTGGATGAACATGCAg                                                                                             <  1:183714/148‑1 (MQ=255)
                                                           gggCATGTCGAACGGGTAATCGGCGAACACCGCGCGCAGCAAGTTCCTGAGACTGCCGTCAAGCCGATAGGTACGGGTTTGCGCGGCGCGCTCGCG‑GCCGCGCATGGAAGGCCGGGTAGTGTGGATGAACATGCAGCCGTCGCTGATc                                                                                 <  5:124970/148‑1 (MQ=255)
                                                                        gtgTAATCGGCGAACACCGAGCGCAGCAAGTTCCTGAGACTGCCGTCAAGCCGATAGGTACGGGTTTGCGCGGCGCGCTCGCG‑GCCGCGCATGGAAGGCCGGGTAGTGTGGATGAACATGCAGCCGTCGCTGATCAGGTCATGGCTGaa                                                                   <  4:262659/147‑1 (MQ=255)
                                                                        gggTAATCGGCGAACACCGCGCGCAGCAAGTTCCTGAGACTGCCGTCAAGCCGATAGGTACGGGTTTGCGCGGCGCGCTCGCG‑GCCGCGCATGGAAGGCCGGGTAGTGTGGATGAACATGCAGCCGTCGCTGATCAGGTCATGGCTg                                                                     >  4:365015/1‑147 (MQ=255)
                                                                        gggTAATCGGCGAACACCGCGCGCAGCAAGTTCCTGAGACTGCCGTCAAGCCGATAGGTACGGGTTTGCGCGGCGCGCTCGCG‑GCCGCGCATGGAAGGCCGGGTAGTGTGGATGAACATGCAGCCGTCGCTGATCAGGTCATGGCTGaa                                                                   <  7:36168/149‑1 (MQ=255)
                                                                          gTAATCGGCGAACACCGCGCGCAGCAAGTTCCTGAGACTGCCGTCAAGCCGATAGGTACGGGTTTGCGCGGCGCGCTCGCG‑GCCGCGCATGGAAGGCCGGGTAGTGTGGATGAACATGCAGCCGTCGCTGATCAGGTCATGGCTGAaga                                                                 <  5:166334/149‑1 (MQ=255)
                                                                             aTCGGCGAACACCGCGCGCAGCAAGTTCCTGAGACTGCCGTCAAGCCGATAGGTACGGGTTTGCGCGGCGCGCTCGCG‑GCCGCGCATGGAAGGCCGGGTAGTGTGGATGAACATGCAGCCGTCGCTGATCAGGTCATGGCTGAAGAgg                                                               <  3:365015/148‑1 (MQ=255)
                                                                             aTCGGCGAACACCGCGCGCAGCAAGTTCCTGAGACTGCCGTCAAGCCGATAGGTACGGGTTTGCGCGGCGCGCTCGCG‑GCCGCGCATGGAAGGCCGGGTAGTGTGGATGAACATGCAGCCGTCGCTGATCAGGTCATGGCTGAAGAGGc                                                              >  6:274755/1‑149 (MQ=255)
                                                                               cGGCGAACACCGCGCGCAGCAAGTTCCTGAGACTGCCGTCAAGCCGATAGGTACGGGTTTGCGCGGCGCGCTCGCG‑GCCGCGCATGGAAGGCCGGGTAGTGTGGATGAACATGCAGCCGTCGCTGATCAGGTCATGGCTGAAGAGGCTg                                                            >  7:96941/1‑149 (MQ=255)
                                                                                     acacCGCGCGCAGCAAGTTCCTGAGACTGCCGTCAAGCCGATAGGTACGGGTTTGCGCGGCGCGCTCGCG‑GCCGCGCATGGAAGGCCGGGTAGTGTGGATGAACATGCAGCCGTCGCTGATCAGGTCATGGCTGAAGAGGCTGCAAAAc                                                      <  3:301340/149‑1 (MQ=255)
                                                                                      cacCGCGCGCAGCAAGTTCCTGAGACTGCCGTCAAGCCGATAGGTACGGGTTTGCGCGGCGCGCTCGCG‑GCCGCGCATGGAAGGCCGGGTAGTGTGGATGAACATGCAGCCGTCGCt                                                                                     <  6:52252/117‑1 (MQ=255)
                                                                                      cacCGCGCGCAGCAAGTTCCTGAGACTGCCGTCAAGCCGATAGGTACGGGTTTGCGCGGCGCGCTCGCG‑GCCGCGCATGGAAGGCCGGGTAGTGTGGATGAACATGCAGCCGACGCt                                                                                     >  5:52252/1‑117 (MQ=255)
                                                                                        ccGCGCGCAGCAAGTTCCTGAGACTGCCGTCAAGCCGATAGGTACGGGTTTGCGCGGCGCGCTCGCG‑GCCGCGCATGGAAGGCCGGGTAGTGTGGATGAACATGCAGCCGTCGCTGATCAGGTCATGGCTGAAGAGGCTGCAAAACAGc                                                   <  3:234982/149‑1 (MQ=255)
                                                                                         cgcgcgCAGCAAGTTCCTGAGACTGCCGTCAAGCCGATAGGTACGGGTTTGCGCGGCGCGCTCGCG‑GCCGCGCATGGAAGGCCGGGTAGTGTGGATGAACATGCAGCCGTCGCTGATCAGGTCATGGCTGAAGAGGCTGCAAAACAGc                                                   >  2:256997/1‑148 (MQ=255)
                                                                                         cgcgcgCAGCAAGTTCCTGAGACTGCCGTCAAGCCGATAGGTACGGGTTTGCGCGGCGCGCTCGCG‑GCCGCGCATGGAAGGCCGGGTAGTGTGGATGAACATGCAGCCGTCGCTGATCAGGTCATGGCTGAAGAGGCTGCAAAACAGc                                                   >  2:70585/1‑148 (MQ=255)
                                                                                          gcgcgcAGCAAGTTCCTGAGACTGCCGTCAAGCCGATAGGTACGGGTTTGCGCGGCGCGCTCGCG‑GCCGCGCATGGAAGGCCGGGTAGTGTGGATGAACATGCAGCCGTCGCTGATCAGGTCATGGCTGAAGAGGCTg                                                            >  6:96432/1‑138 (MQ=255)
                                                                                          gcgcgcAGCAAGTTCCTGAGACTGCCGTCAAGCCGATAGGTACGGGTTTGCGCGGCGCGCTCGCG‑GCCGCGCATGGAAGGCCGGGTAGTGTGGATGAACATGCAGCCGTCGCTGATCAGGTCATGGCTGAAGAGGCTg                                                            <  5:96432/138‑1 (MQ=255)
                                                                                          gcgcgcAGCAAGTTCCTGAGACTGCCGTCAAGCCGATAGGTACGGGTTTGCGCGGCGCGCTCGCG‑GCCGCGCATGGAAGGCCGGGTAGTGTGGATGAACATGCAGCCGTCGCTGATCAGGTCATGGCTGAAGAGGCTGCAAAACAGCGa                                                 <  5:274755/149‑1 (MQ=255)
                                                                                          gcgcgcAGCAAGTTCCTGAGACTGCCGTCAAGCCGATAGGTACGGGTTTGCGCGGCGCGCTCGCG‑GCCGCGCATGGAAGGCCGGGTAGTGTGGATGAACATGCAGCCGTCGCTGATCAGGTCATGGCTGAAGAGGCTGCAAAACAGCGa                                                 <  1:256997/149‑1 (MQ=255)
                                                                                          agtctcaggaACTTGCTGAGACTGCCGTCAAGCCGATTGGTACGGGTTTGCGCGGCGCGCTCGCG‑GCCGCGCATGGAAGGCCGGGTAGTGTGGATGAACATGCAGCCGTCGCTGATCAGGTCATGGCTGAAGAGGCTGCAAAACAGc                                                   >  1:65725/6‑147 (MQ=255)
                                                                                                agcaAGTTCCTGAGACTGCCGTCAAGCCGATAGGTACGGGTTTGCGCGGCGCGCTCGCG‑GCCGCGCATGGAAGGCCGGGTAGTGTGGATGAACATGCAGCCGTCGCTGATCAGGTCATGGCTGAAGAGGCTGCAAAACAGCGAGTTGTa                                           <  5:163940/149‑1 (MQ=255)
                                                                                                 gcaAGTTCCTGAGACTGCCGTCAAGCCGATAGGTACGGGTTTGCGCGGCGCGCTCGCG‑GCCGCGCATGGAAGGCCGGGTAGTGTGGATGAACATGCAGCCGTCGCTGATCAGGTCATGGCTGAAGAGGCTGCAAAACAGCGAGTTGTAc                                          <  8:96941/149‑1 (MQ=255)
                                                                                                     gTTCCTGAGACTGCCGTCAAGCCGATAGGTACGGGTTTGCGCGGCGCGCTCGCG‑GCCGCGCATGGAAGGCCGGGTAGTGTGGATGAACATGCAGCCGTCGCTGATCAGGTCATGGCTGAAGAGGCTGCAAAACAGCGAGTTGTACCAgg                                      <  4:359396/149‑1 (MQ=255)
                                                                                                      ttCATGAGACTGCCGTCAAGCCGATAGGTACGGGTTTGCGCGGCGCGCTCGCG‑GCCGCGCATGGAAGGCCGGGAAGTGTGGATGAACATGCAGACGACGATGATCAGGTCATGGATGAAGAGGCTGCAAAACAGCGAGAAGTACAAgat                                     >  4:370347/1‑147 (MQ=255)
                                                                                                         cTGAGACTGCCGTCAAGCCGATAGGTACGGGTTTGCGCGGCGCCCTCGCG‑GCCGCGCAAGGAAGGCCGGGTAGTGTGGATGAACCTTCAGCCGTCGCTGATAAGGGCAAGGCTGCAGACGCTGCAAAACACCGAGTTGTACCAGGtcag                                  >  2:157806/1‑147 (MQ=255)
                                                                                                           gagaCTGCCGTCAAGCCGATAGGTACGGGTTTGCGCGGCGCGCTCGCG‑GCCGCGCATGGAAGGCCGGGTAGTGTGGATGAACATGCAGCCGTCGCTGATCAGGTCATGGCTGAAGAGGCTGCAAAACAGCGAGTTGTACCAGGTCTCTg                                >  5:174352/1‑149 (MQ=255)
                                                                                                                  ccGTCAAGCCGATAGGTACGGGTTTGCGCGGCGCGCTCGCG‑GCCGCGCATGGAAGGCCGGGTAGTGTGGATGAACATGCAGCCGTCGCTGATCAGGTCATGGCTGAAGAGGCTGCAAAACAGCGAGTTGTACCAGGTCTCTGCCAACtc                         >  5:287420/1‑149 (MQ=255)
                                                                                                                  ccGTCAAGCCGATAGGTACGGGTTTGCGCGGCGCGCTCGCG‑GCCGCGCATGGAAGGCCGGGTAGTGTGGATGAACATGCAGCCGTCGCTGATCAGGTCATGGCTGAAGAGGCTGCAAAACAGCGAGTTGTACCAGGTCTCTGCCAACtc                         <  3:73934/149‑1 (MQ=255)
                                                                                                                  ccGTCAAGCCGATAGGTACGGGTTTGCGCGGCGCGCTCGCG‑GCCGCGCATGGAAGGCCGGGTAGTGTGGATGAACATGCAGCCGTCGCTGATCAGGTCATGGCTGAAGAGGCTGCAAAACAGCGAGTTGTACCAGGTCTCTGCCAACtc                         >  3:249394/1‑149 (MQ=255)
                                                                                                                         gCCGATAGGTACGGGATTGCGCGGCGCGCTCGCG‑GCCGCGCATGGAAGGCCGTGTTGTGTGGATTAACATGCAGCCGTCGCTGATCTGGTCTTGGCTGAAGAGGCTGCAAAACAGCGAGTTGTACCAGGTCTCTGCCAACTCGTCGTcc                  <  6:317751/149‑1 (MQ=255)
                                                                                                                          ccGATAGGTACGGGTTTGCGCGGCGCGCTCGCG‑GCCGCGCATGGAAGGCCGGGTAGTGTGGATGAACATGCAGCCGTCGCTGATCAGGTCATGGCTGAAGAGGCTGCAAAACAGCGAGTTGTACCAGGTCTCTGCCAACTCGTCGTCCa                 <  4:249394/149‑1 (MQ=255)
                                                                                                                                 gTACGGGTTTGCGCGGCGCGCTCGCG‑GCCGCGCATGGAAGGCCGGGTAGTGTGGATGAACATGCAGCCGTCGCTGATCAGGTCATGGCTGAAGAgg                                                               >  7:75720/1‑96 (MQ=255)
                                                                                                                                 gTACGGGTTTGCGCGGCGCGCTCGCG‑GCCGCGCATGGAAGGCCGGGTAGTGTGGATGAACATGCAGCCGTCGCTGATCAGGTCATGGCTGAAGAgg                                                               <  8:75720/96‑1 (MQ=255)
                                                                                                                                  tACGGGTTTGCGCGGCGCGCTCGCG‑GCCGCGCATGGAAGGCCGGGTAGTGTGGATGAACATGCAGCCGTCGCTGATCAGGTCATGGCTGAAGAGGCTGCAAAACAGCGAGTTGTACCAGGTCTCTGCCAACTCGTCGTCCAGGCggggg         <  7:261123/149‑1 (MQ=255)
                                                                                                                                          tGCGCGGCGCGCTCGCG‑GCCGCGCATGGAAGGCCGGGTAGTGTGGATGAACATGCAGCCGTCGCTGATCAGGTCATGGCTGAAGAGGCTGCAAAACAGCGAGTTGTACCAGGTCTCTGCCAACTCGTCGTCCAGGCGGGGGTCGATCa  <  8:258918/148‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                            
GGCAGGCATTCGCGCAGTTGTTCTTCCAGCCGCGCCAGGTCGCCTTCCAGGTCGCCATAGGGCATGTCGAACGGGTAATCGGCGAACACCGCGCGCAGCAAGTTCCTGAGACTGCCGTCAAGCCGATAGGTACGGGTTTGCGCGGCGCGCTCGCG‑GCCGCGCATGGAAGGCCGGGTAGTGTGGATGAACATGCAGCCGTCGCTGATCAGGTCATGGCTGAAGAGGCTGCAAAACAGCGAGTTGTACCAGGTCTCTGCCAACTCGTCGTCCAGGCGGGGGTCGATCA  >  NC_002947/5185903‑5186188

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑