Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,907,912 Δ1 bp 6.8% coding (55/1095 nt) spuE ← spermidine/putrescine ABC transporter substrate‑binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,907,9120C. 6.8% 187.3 / 7.1 59coding (55/1095 nt)spuEspermidine/putrescine ABC transporter substrate‑binding protein
Reads supporting (aligned to +/- strand):  ref base C (25/30);  new base . (2/2);  total (27/32)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.01e-01

TTCCAGGGTTTCGTTGGAGTCGAACACATCTTGCACAGGCTTGATGCCCGTGGCTTTCTCGAAGTCCGCGAGTGTGGTGGGGCCGATGTAGTCGGACCAGTTATAGAAGTGCACCGTCGGCGCCGCTTGGACGCT‑GCATGCCAGCGTCAGGCCCGCGCCAGCCATCAAGGCCTTGCGGAATACAGAAATAGACAAGT‑‑GGGGGTCCTCACAATCAGTGCCTGGGTAGCGACAATGCTGCCGCACACGCAAAACCGGCGCGCAACTTAACTTCGCAGCTTCGATG  >  NC_002947/5907772‑5908054
                                                                                                                                             |                                                                                                                                                
ttCCAGGATTTCGTTGGAGTCGAACACATCTTCCACAGTATTGATGCCGGTTGCTTTCACGAAGACCGCGAGAGTGGTGGGGACGATGTAGTCGGACCAGTTAAAGAACTGCACCGTCGGCGCCGCTTGGACGCA‑GCATACCAGCGt                                                                                                                                            >  6:137851/1‑147 (MQ=255)
  ccAGGGTTTCGTTGGAGTCGAACACATCTTGCACAGGCTTGATGCCCGTGGCTTTCTCGAAGTCCGCGAGTGTGGTGGGGCCGATGTAGTCGGACCAGTTATAGAAGTGCACCGTCGGCGCCGCTTGGACGCT‑GCATGCCAGCGTCAg                                                                                                                                         <  5:137851/148‑1 (MQ=255)
  ccAGGGTTTCGTTGGAGTCGAACACATCTTGCACAGGCTTGATGCCCGTGGCTTTCTCGAAGTCCGCGAGTGTGGTGGGGCCGATGTAGTCGGACCAGTTATAGAAGTGCACCGTCGGCGCCGCTTGGACGCT‑GCATGCCAGCGTCAg                                                                                                                                         <  3:112352/148‑1 (MQ=255)
  ccAGGGTTTCGTTGGAGTCGAACACATCTTGCACAGGCTTGATGCCCGTGGCTTTCTCGAAGTCCGCGAGTGTGGTGGGGCCGATGTAGTCGGACCAGTTATAGAAGTGCACCGTCGGCGCCGCTTGGACGCT‑GCATGCCAGCGTCAg                                                                                                                                         <  4:355750/148‑1 (MQ=255)
   ctgggTTTCGTTGGAGTCGAACACATCTTGCTCAGGCTTGATGCCCGTGGCTTTCTCGATGTCCGCGAGTGTGGTGGGGCCGATGTAGTCGGACCAGTTATAGAAGTGCACCGTCGGCGCCGCTTGGACGCTGGCATG‑CAGCGTccaa                                                                                                                                        <  8:210042/146‑4 (MQ=255)
   cAGGGTTTCGTTGGAGTCGAACACATCTTGCACAGGCTTGATGCCCGTGGCTTTCTCGAAGTCCGCGAGTGTGGTGGGGCCGATGTAGTCGGACCAGTTATAGAAGTGCACCGTCGGCGCCGCTTGGACGCTGGCATG‑CAGCGTccaa                                                                                                                                        <  2:111087/148‑4 (MQ=255)
     gggTTTCGTTGGAGTCGAACACATCTTGCACAGGCTTGATGCCCGTGGCTTTCTCGAAGTCCGCGAGTGTGGTGGGGCCGATGTAGTCGGACCAGTTATAGAAGTGCACCGTCGGCGCCGCTTGGACGCT‑GCATGCCAGCGTCAGGccc                                                                                                                                     >  8:85486/1‑149 (MQ=255)
            gTTGGAGTCGAACACATCTTGCACAGGCTTGATGCCCGTGGCTTTCTCGAAGTCCGCGAGTGTGGTGGGGCCGATGTAGTCGGACCAGTTATAGAAGTGCACCGTCGGCGCCGCTTGGACGCT‑GCATGCCAGCGTCAGGCCCGCgcca                                                                                                                               <  7:177812/148‑1 (MQ=255)
                 aGTCGAACACATCTTGCACAGGCTTGATGCCCGTGGCTTTCTCGAAGTCCGCGAGTGTGGTGGGGCCGATGTAGTCGGACCAGTTATAGAAGTGCACCGTCGGCGCCGCTTGGACGCC‑GCATGCCAGCGTCAGGCCCGCGCCAGCCATc                                                                                                                         <  1:22820/149‑1 (MQ=255)
                      aaCACATCTTGCACAGGCTTGATGCCCGTGGCTTTCTCGAAGTCCGCGAGTGTGGTGGGGCCGATGTAGTCGGACCAGTTATAGAAGTGCACCGTCGGCGCCGCTTGGACGCT‑GCATGCCAGCGTCAGGCCCGCGCCAGCCATCAAGGc                                                                                                                    >  6:158834/1‑149 (MQ=255)
                      aaCACATCTTGCACAGGCTTGATGCCCGTGGCTTTCTCGAAGTCCGCGAGTGTGGTGGGGCCGATGTAGTCGGACCAGTTATAGAAGTGCACCGTCGGCGCCGCTTGGACGCT‑GCATGCCAGCGTCAGGCCCGCGCCAGCCATCAAGGc                                                                                                                    >  5:300679/1‑149 (MQ=255)
                         acaTCTTGCACAGGCTTGATGCCCGTGGCTTTCTCGAAGTCCGCGAGTGTGGTGGGGCCGATGTAGTCGGACCAGTTATAGAAGTGCACCGTCGGCGCCGCTTGGACGCT‑GCATGCCAGCGTCAGGCCCGCGCCAGCCATCAAGGCCtt                                                                                                                 <  3:262732/149‑1 (MQ=255)
                           aTCTTGCACAGGCTTGATGCCCGTGGCTTTCTCGAAGTCCGCGAGTGTGGTGGGGCCGATGTAGTCGGACCAGTTATAGAAGTGCACCGTCGGCGCCGCTTGGACGCT‑GCATGCCAGCGTCAGGCCCGCGCCAGCCATCAAGGCCTTGc                                                                                                               >  7:175058/1‑149 (MQ=255)
                           aTCTTGCACAGGCTTGATGCCCGTGGCTTTCTCGAAGTCCGCGAGTGTGGTGGGGCCGATGTAGTCGGACCAGTTATAGAAGTGCACCGTCGGCGCCGCTTGGACGCT‑GCATGCCAGCGTCAGGCCCGCGCCAGCCATCAAGGCCTTGc                                                                                                               <  2:325701/149‑1 (MQ=255)
                           aTCTTGCACAGGCTTGATGCCCGTGGCTTTCTCGAAGTCCGCGAGTGTGGTGGGGCCGATGTAGTCGGACCAGTTATAGAAGTGCACCGTCGGCGCCGCTTGGACGCT‑GCATGCCAGCGTCAGGCCCGCGCCAGCCATCAAGGCCTTGc                                                                                                               >  5:112958/1‑149 (MQ=255)
                           aTCTTGCACAGGCTTGATGCCCGTGGCTTTCTCGAAGTCCGCGAGTGTGGTGGGGCCAATGTAGTCGGACCAGTTATAGAAGTGCACCGTCGGCGCCGCTTGGACGCT‑GCATGCCAGCGTCAGGCCCGCGCCAGCCATCAAGGCCTTGc                                                                                                               >  1:114573/1‑149 (MQ=255)
                            tCTTGCACAGGCTTGATGCCCGTGGCTTTCTCGAAGTCCGCGAGTGTGGTGGGGCCGATGTAGTCGGACCAGTTATAGAAGTGCACCGTCGGCGCCGCTTGGACGCT‑GCATGCCAGCGTCAGGCCCGCGCCAGCCATCAAGGCCTTGCg                                                                                                              >  3:148637/1‑149 (MQ=255)
                                gCACAGGCTTGATGCCCGTGGCTTTCTCGAAGTCCGCGAGTGTGGTGGGGCCGATGTAGTCGGACCAGTTATAGAAGTGCACCGTCGGCGCCGCTTGGACGCT‑GCATGCCAGCGTCAGGCCCGCGCCAGCCATCAAGGCCTTGCg                                                                                                              <  6:300679/145‑1 (MQ=255)
                                gCACAGGCTTGATGCCCGTGGCTTTCTCGAAGTCCGCGAGTGTGGTGGGGCCGATGTAGTCGGACCAGTTATAGAAGTGCACCGTCGGCGCCGCTTGGACGCT‑GCATGCCAGCGTCAGGCCCGCGCCAGCCATCAAGGCCTTGCGGaa                                                                                                           >  3:1372/1‑148 (MQ=255)
                                 cacaGGCTTGATGCCCGTGGCTTTCTCGAAGTCCGCGAGTGTGGTGGGGCCGATGTAGTCGGACCAGTTATAGAAGTGCACCGTCGGCGCCGCTTGGACGCT‑GCATGCCAGCGTCAGGCCCGCGCCAGCCATCAAGGCCTTGCGGAAt                                                                                                          <  6:246222/148‑1 (MQ=255)
                                 cacaGGCTTGATGCCCGTGGCTTTCTCGAAGTCCGCGAGTGTGGTGGGGCCGATGTAGTCGGACCAGTTATAGAAGTGCACCGTCGGCGCCGCTTGGACGCT‑GCATGCCAGCGTCAGGCCCGCGCCAGCCATCAAGGCCTTGCGGAAt                                                                                                          <  4:148637/148‑1 (MQ=255)
                                 cacaGGCTTGATGCCCGTGGCTTTCTCGAAGTCCGCGAGTGTGGTGGGGCCGATGTAGTCGGACCAGTTATAGAAGTGCACCGTCGGCGCCGCTTGGACGCT‑GAATGCCAGCGTCAGGCCCGCGCCAGCCATCAAGGCCTTGCGGAATa                                                                                                         >  8:354430/1‑149 (MQ=255)
                                      gCTTGATGCCCGTGGCTTTCTCGAAGTCCGCGAGTGTGGTGGGGCCGATGTAGTCGGACCAGTTATAGAAGTGCACCGTCGGCGCCGCTTGGACGCT‑GCATGCCAGCGTCAGGCCCGCGCCAGCCATCAAGGCCTTGCGGAATACAGaa                                                                                                    <  6:112958/149‑1 (MQ=255)
                                      gCTTGATGCCCGTGGCTTTCTCGAAGTCCGCGAGTGTGGTGGGGCCGATGTAGTCGGACCAGTTATAGAAGTGCACCGTCGGCGCCGCTTGGACGCT‑GCATGCCAGCGTCAGGCCCGCGCCAGCCATCAAGGCCTTGCGGAATACAGa                                                                                                     >  8:114450/1‑148 (MQ=255)
                                        ttGATGCCCGTGGCTTTCTCGAAGTCCGCGAGTGTGGTGGGGCCGATGTAGTCGGACCAGTTATAGAAGTGCACCGTCGGCGCCGCTTGGACGCT‑GCATGCCAGCGTCAGGCCCGCGCCAGCCATCAAGGCCTTGCg                                                                                                              >  2:306007/1‑137 (MQ=255)
                                        ttGATGCCCGTGGCTTTCTCGAAGTCCGCGAGTGTGGTGGGGCCGATGTAGTCGGACCAGTTATAGAAGTGCACCGTCGGCGCCGCTTGGACGCT‑GCATGCCAGCGTCAGGCCCGCGCCAGCCATCAAGGCCTTGCg                                                                                                              <  1:306007/137‑1 (MQ=255)
                                        ttGATGCCCGTGGCTTTCTCGAAGTCCGCGAGTGTGGTGGGGCCGATGTAGTCGGACCAGTTATAGAAGTGCACCGTCGGCGCCGCTTGGACGCT‑GCATGCCAGCGTCAGGCCCGCGCCAGCCATCAAGGCCTTGCGGAATACAGaaa                                                                                                   >  2:40399/1‑148 (MQ=255)
                                          gATGCCCGTGGCTTTCTCGAAGTCCGCGAGTGTGGTGGGGCCGATGTAGTCGGACCAGTTATAGAAGTGCACCGTCGGCGCCGCTTGGACGCT‑GCATGCCAGCGTCAGGCCCGCGCCAGCCATCAACGCCTTGCGGAATACAGaaa                                                                                                   >  4:338437/1‑146 (MQ=255)
                                           aTGCCCGTGGCTTTCTCGAAGTCCGCGAGTGTGGTGGGGCCGATGTAGTCGGACCAGTTATAGAAGTGCACCGTCGGCGCCGCTTGGACGCT‑GCATGCCAGCGTCAGGCCCGCGCCAGCCATCAAGGCCTTGCGGAATACAGAAATAGa                                                                                               <  3:280939/149‑1 (MQ=255)
                                            tGCCCGTGGCTTTCTCGAAGTCCGCGAGTGTGGTGGGGCCGATGTAGTCGGACCAGTTATAGAAGTGCACCGTCGGCGCCGCTTGGACGCT‑GCATGCCAGCGTCAGGCCCGCGCCAGCCATCAAGGCCTTGCGGAATACAGAAATAGa                                                                                               >  4:217948/1‑148 (MQ=255)
                                              cccGTGGCTTTCTCGAAGTCCGCGAGTGTGGTGGGGCCGATGTAGTCGGACCAGTTATAGAAGTGCACCGTCGGCGCCGCTTGGACGCT‑GCATGCCAGCGTCAGGCCCGCGCCAGCCATCAAGGCCTTGCGGAATAGAGAAATAGACaa                                                                                            <  5:45701/149‑1 (MQ=255)
                                                cGTGGCTTTCTCGAAGTCCGCGAGTGTGGTGGGGCCGATGTAGTCGGACCAGTTATAGAAGTGCACCGTCGGCGCCGCTTGGACGCT‑GCATGCCAGCGTCAGGCCCGCGCCAGCCATCAAGGCCTTGCGGAATACAGAAATAGACAAGt                                                                                          >  4:57633/1‑149 (MQ=255)
                                                  tGGCTTTCTCGAAGTCCGCGAGTGTGGTGGGGCCGATGTAGTCGGACCAGTTATAGAAGTGCACCGTCGGCGCCGCTTGGACGCT‑GCATGCCAGCGTCAGGCCCGCGCCAGCCATCAAGGCCTTGCGGAATACAGAAATAGACAAGT‑‑gg                                                                                      <  5:158834/149‑1 (MQ=255)
                                                    gCTTTCTCGAAGTCCGCGAGTGTGGTGGGGCCGATGTAGTCGGACCAGTTATAGAAGTGCACCGTCGGCGCCGCTTGGACGCT‑GCATGCCAGCGTCAGGCCCGCGCCAGCCATCAAGGCCTTGCGGAATACAGAAATAGACAAGT‑‑gggg                                                                                    <  7:114450/149‑1 (MQ=255)
                                                    gCTTTCTCGAAGTCCGCGAGTGTGGTGGGGCCGATGTAGTCGGACCAGTTATAGAAGTGCACCGTCGGCGCCGCTTGGACGCT‑GCATGCCAGCGTCAGGCCCGCGCCAGCCATCAAGGCCTTGCGGAATACAGAAATAGACAAGT‑‑gggg                                                                                    <  7:85486/149‑1 (MQ=255)
                                                          tcGAAGTCCGCGAGTGTGGTGGGGCCGATGTAGTCGGACCAGTTATAGAAGTGCACCGTCGGCGCCGCTTGGACGCT‑GCATGCCAGCGTCAGGCCCGCGCCAGCCATCAAGGCCTTGCGGAATACAGAAATAGACAAGT‑‑GGGGGTCCt                                                                               >  7:344418/1‑148 (MQ=255)
                                                          tcGAAGTCCGCGAGTGTGGTGGGGCCGATGTAGTCGGACCAGTTATAGAAGTGCACCGTCGGCGCCGCTTGGACGCT‑GCATGCCAGCGTCAGGCCCGCGCCAGCCATCAAGGCCTTGCGGAATACAGAAATAGACAAGT‑‑GGGGGTCCt                                                                               >  2:165342/1‑148 (MQ=255)
                                                            gAAGTCCGCGAGTGTGGTGGGGCCGATGTAGTCGGACCAGTTATAGAAGTGCACCGTCGGCGCCGCTTGGACGCT‑GCATGCCAGCGTCAGGCCCGCGCCAGCCATCAAGGCCTTGCGGAATACAGAAATAGACAAGT‑‑GGGGGTCCTca                                                                             <  5:147236/148‑1 (MQ=255)
                                                                tCCGCGAGTGTGGTGGGGCCGATGTAGTCGGACCAGTTATAGAAGTGCACCGTCGGCGCCGCTTGGACGCT‑GCATGCCAGCGTCAGGCCCGCGCCAGCCATCAAGGCCTTGCGGAATACAGAAATAGACAAGT‑‑GGGGGTCCTCACAATc                                                                        <  8:175058/149‑1 (MQ=255)
                                                                  cgcgAGTGTGGTGGGGCCGATGTAGTCGGACCAGTTATAGAAGTGCACCGTCGGCGCCGCTTGGACGCT‑GCATGCCAGCGTCAGGCCCGCGCCAGCCATCAAGGCCTTGCGGAATACAGAAATAGACAAGT‑‑GGGGGTCCTCACAATCa                                                                       <  8:344418/148‑1 (MQ=255)
                                                                   gcgAGTGTGGTGGGGCCGATGTAGTCGGACCAGTTATAGAAGTGCACCGTCGGCGCCGCTTGGACGCT‑GCATGCCAGCGTCAGGCCCGCGCCAGCCATCAAGGCCTTGCGGAATACAGAAATAGACAAGT‑‑GGGGGTCCTCACAATCAGt                                                                     <  8:326170/149‑1 (MQ=255)
                                                                   gcgAGTGTGGTGGGGCCGATGTAGTCGGACCAGTTATAGAAGTGCACCGTCGGCGCCGCTTGGACGCT‑GCATGCCAGCGTCAGGCCCGCGCCAGCCATCAAGGCCTTGCGGAATACAGAAATAGACAAGT‑‑GGGGGTCCTCACAATCAGt                                                                     <  6:45152/149‑1 (MQ=255)
                                                                          tggtggGGCCGATGTAGTCGGACCAGTTATAGAAGTGCACCGTCGGCGCCGCTTGGACGCT‑GCATGCCAGCGTCAGGCCCGCGCCAGCCATCAAGGCCTTGCGGAATACAGAAATAGACAAGT‑‑GGGGGTCCTCACAATCAGTGCCTggg                                                              <  1:165342/149‑1 (MQ=255)
                                                                           ggtggGGCCGATGTAGTCGGACCAGTTATAGAAGTGCACCGTCGGCGCCGCTTGGACGCT‑GCATGCCAGCGTCAGGCCCGCGCCAGCCATCAAGGCCTTGCGGAATACAGAAATAGACAAGT‑‑GGGGGTCCTCACAATCAGTGCCTggg                                                              <  3:338437/148‑1 (MQ=255)
                                                                                    gATGTAGTCGGACCAGTTATAGAAGTGCACCGTCGGCGCCGCTTGGACGCT‑GCATGCCAGCGTCAGGCCCGCGCCAGCCATCAAGGCCTTGCGGAATACAGAAATAGACAAGT‑‑GGGGGTTCTCACAATCAGTGCCTGGGTAGCGACAAt                                                    >  5:249167/1‑149 (MQ=255)
                                                                                     aTGTAGTCGGACCAGTTATAGAAGTGCACCGTCGGCGCCGCTTGGACGCT‑GCATGCCAGCGTCAGGCCCGCGCCAGCCATCAAGGCCTTGCGGAATACAGAAATAGACAAGT‑‑GGGGGTCCTCACAATCAGTGCCTGGGTAGCGACAAtg                                                   <  2:114573/149‑1 (MQ=255)
                                                                                          gTCGGACCAGTTATAGAAGTGCACCGTCGGCGCCGCTTGGACGCT‑GCATGCCAGCGTCAGGCCCGCGCCAGCCATCAAGGCCTTGCGGAATACAGAAATAGACAAGT‑‑GGGGGTCCTCACAATCAGTGCCTGGGTAGCGACAAtgctgc                                               <  4:1372/148‑1 (MQ=255)
                                                                                              gACCAGTTATAGAAGTGCACCGTCGGCGCCGCTTGGACGCT‑GCATGCCAGCGTCAGGCCCGCGCCAGCCATCAAGGCCTTGCGGAATACAGAAATAGACAAGT‑‑GGGGGTCCTCACAATCAGTGCCTGGGTAGCGACAATGCTGCCGca                                           >  3:271457/1‑148 (MQ=255)
                                                                                                  aGTTATAGAAGTGCACCGTCGGCGCCGCTTGGACGCT‑GCATGCCAGCGTCAGGCCCGCGCCAGCCATCAAGGCCTTGCGGAATACAGAAATAGACAAGT‑‑GGGGGTCCTCACAATCAGTGCCTGGGTAGCGACAATGCTGCCGCACACGc                                      <  3:217948/149‑1 (MQ=255)
                                                                                                      ataGAAGTGCACCGTCGGCGCCGCTTGGACGCT‑GCATGCCAGCGTCAGGCCCGCGCCAGCCATCAAGGCCTTGCGGAATACAGAAATAGACAAGT‑‑GGGGGTCCTCACAATCAGTGCCTGGGTAGCGACAATGCTGCCGCACCCGCaaaa                                  >  4:198333/1‑149 (MQ=255)
                                                                                                      ataGAAGTGCACCGTCGGCGCCGCTTGGACGCT‑GCATGCCAGCGTCAGGCCCGCGCCAGCCATCAAGGCCTTGCGGAATACAGAAATAGACAAGT‑‑GGGGGTCCTCACAATCAGTGCCTGGGTAGCGACAATGCTGCCGCACACGCaaaa                                  >  8:346149/1‑149 (MQ=255)
                                                                                                         gAAGTGCACAGTCGGCGCCGCTTGGACGCT‑GCATGCCAGCGTCAGGCCCGCGCCAGCCATCAAGGCCTTGCGGAATACAGAAATAGACAAGT‑‑GGGGGTCCTCACAATCAGTGCCTGGGTAGCGACAATGCTGCCGCACACGCAAAACCg                               <  4:205112/149‑1 (MQ=255)
                                                                                                            gTGCACCGTCGGCGCCGCTTGGACGCT‑GCATGCCAGCGTCAGGCCCGCGCCAGCCATCAAGGCCTTGCGGAAATCAAAAATAGACAAGTAGGGGGGTCCTCACAATCAGTGCCTGGGTAGCGACAATGCTGCCGCACACGCAAAACCgg                              >  1:115947/1‑149 (MQ=255)
                                                                                                               cACCGTCGGCGCCGCTTGGACGCT‑GCATGCCAGCGTCAGGCCCGCGCCAGCCATCAAGGCCTTGCGGAATACAGAAATAGACAAGT‑‑GGGGGTCCTCACAATCAGTGCCTGGGTAGCGACAATGCTGCCGCACACGCAAAACCGgcgcg                          >  4:82055/1‑148 (MQ=255)
                                                                                                               cACCGTCGGCGCCGCTTGGACGCT‑GCATGCCAGCGTCAGGCCCGCGCCAGCCATCAAGGCCTTGCGGAATACAGAAATAGACAAGT‑‑GGGGGTCCTCACAATCAGTGCCTGGGTAGCGACAATGCTGCCGCACACGCAAAACCGgcgcg                          <  3:57633/148‑1 (MQ=255)
                                                                                                                            gcTTGGACGCT‑GCATGCCAGCGTCAGGCCCGCGCCAGCCATCAAGGCCTTGCGGAATACAGAAATAGACAAGT‑‑GGGGGTTCTCACAATCAGTGCCTGGGTAGCGACAATGCTGCCGCACACGCAAAACCGGCGCGCAACTTAACTTCGc            <  6:249167/149‑1 (MQ=255)
                                                                                                                             gcctgACGCTGGCATG‑CAGCGTCAGGCCCGCGCCAGCCATCAAGGCCTTGCGGAATACAGAAATAGACAAGT‑‑GGGGGTCCTCACAATCAGTGCCTGGGTAGCGACAATGCTGCCGCACACGCAAAACCGGCGCGCAACTTAACTTCg             >  6:138974/5‑147 (MQ=255)
                                                                                                                               ctgACGCTGGCATG‑CAGCGTCAGGCCCGCGCCAGCCATCAAGGCCTTGCGGAATACAGAAATAGACAAGT‑‑GGGGGTCCTCACAATCAGTGCCTGGGTAGCGACAATGCTGCCGCACACGCATAACCGGCGCGCAACTTACCTTCGCAGc         >  5:376182/3‑149 (MQ=255)
                                                                                                                                      t‑GCATGCCAGCGTCAGGCCCGCGCCAGCCATCAAGGCCTTGCGGAATACAGAAATAGACAAGT‑‑GGGGGTCCTCACAATCAGTGCCTGGGTAGCGACAATGCTGCCGCACACGCAAAACCGGCGCGCAACTTAACTTCGCAGCTTCGATg  >  4:94260/1‑149 (MQ=255)
                                                                                                                                             |                                                                                                                                                
TTCCAGGGTTTCGTTGGAGTCGAACACATCTTGCACAGGCTTGATGCCCGTGGCTTTCTCGAAGTCCGCGAGTGTGGTGGGGCCGATGTAGTCGGACCAGTTATAGAAGTGCACCGTCGGCGCCGCTTGGACGCT‑GCATGCCAGCGTCAGGCCCGCGCCAGCCATCAAGGCCTTGCGGAATACAGAAATAGACAAGT‑‑GGGGGTCCTCACAATCAGTGCCTGGGTAGCGACAATGCTGCCGCACACGCAAAACCGGCGCGCAACTTAACTTCGCAGCTTCGATG  >  NC_002947/5907772‑5908054

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑