Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 557,336:1 +A 8.4% intergenic (+79/‑130) rpsN → / → rpsH 30S ribosomal protein S14/30S ribosomal protein S8

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947557,3361.A 8.4% 366.4 / 26.3 95intergenic (+79/‑130)rpsN/rpsH30S ribosomal protein S14/30S ribosomal protein S8
Reads supporting (aligned to +/- strand):  ref base . (43/44);  new base A (4/4);  total (47/48)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCCGTAACATGCTGCGTCAAGCTGCAATGCGCGGTGACGTACCAGGTCTGGTCAAGGCCTCCTGGTAATCGCTGCAGGCAAGGCGCGGGTGGAAGGGGAGCAATCTTCCGACC‑GCCGGTGACCTTGCCAACAAACAAGCCCCTATAT‑GGGGCTTGTTTCGTTTCTGCCTTGTGTCTAGAA‑TGACCGGCTCACCTGAGCCTGGGTTTTTGCCCAAACGGTTCGGGTGGTTGTCATAGCCGCAAGGCTAATAATTCTTGTATCAGGAGCATCTAGCCCATGAGTATGCAGGACCC  >  NC_002947/557190‑557482
                                                                                                                                                    |                                                                                                                                                   
gCCGTAACATGCTGCGTCAAGCTGCAATGCGCGGTGACGTACCAGGTCTGGTCAAGGCCTCCTGGTAATCGCTGCAGGCAAGGCGCGGGTGGAAGGGGAGCAATCTTCCGACC‑GCCGGTGACCTTGCCAACAAACAAGCCCCTATAT‑gg                                                                                                                                                   <  1:257596/149‑1 (MQ=255)
     aaCATGCTGCGTCAAGCTGCAATGCGCGGTGACGTACCAGGTCTGGTCAAGGCCTCCTGGTAATCGCTGCAGGCAAGGCGCGGGTGGAAGGGGAGCAATCTTCCGACC‑GCCGGTGACCTTGCCAACAAACAAGCCCCTATAT‑GGGGCtt                                                                                                                                              >  7:162715/1‑149 (MQ=255)
      aCATGCTGCGTCAAGCTGCAATGCGCGGTGACGTACCAGGTCTGGTCAAGGCCTCCTGGTAATCGCTGCAGGCAAGGCGCGGGTGGAAGGGGAGCAATCTTCCGACC‑GCCGGTGACCTTGCCAACAAACAAGCCCCTATAT‑GGGGCtt                                                                                                                                              <  8:162715/148‑1 (MQ=255)
       cATGCTGCGTCAAGCTGCAATGCGCGGTGACGTACCAGGTCTGGTCAAGGCCTCCTGGTAATCGCTGCAGGCAAGGCGCGGGTGGAAGGGGAGCAATCTTCAGACC‑GCCGGTGACCTTGCCAACAAACAAGCCCC‑ATATAGGGGCTTgt                                                                                                                                            >  6:362789/1‑149 (MQ=255)
        aTGCTGCGTCAAGCTGCAATGCGCGGTGACGTACCAGGTCTGGTCAAGGCCTCCTGGTAATCGCTGCAGGCAAGGCGCGGGTGGAAGGGGAGCAATCTTCCGACC‑GCCGGTGACCTTGCCAACAAACAAGCCCCTATAT‑GGGGCTTgtt                                                                                                                                           >  1:319534/1‑149 (MQ=255)
        aTGCTGCGTCAAGCTGCAATGCGCGGTGACGTACCAGGTCTGGTCAAGGCCTCCTGGTAATCGCTGCAGGCAAGGCGCGGGTGGAAGGGGAGCAATCTTCCGACC‑GCCGGTGACCTTGCCAACAAACAAGCCCCTATAT‑GGGGCTTgtt                                                                                                                                           >  5:142681/1‑149 (MQ=255)
         tgctgcGTCAAGCTGCAATGCGCGGTGACGTACCAGGTCTGGTCAAGGCCTCCTGGTAATCGCTGCAGGCAAGGCGCGGGTGGAAGGGGAGCAATCTTCCGACC‑GCCGGTGACCTTGCCAACAAACAAGCCCC‑ATATAGGGGCTTgttt                                                                                                                                          <  5:362789/149‑1 (MQ=255)
           ctgcGTCAAGCTGCAATGCGCGGTGACGTACCAGGTCTGGTCAAGGCCTCCTGGTAATCGCTGCAGGCAAGGCGCGGGTGGAAGGGGAGCAATCTTCCGACC‑GCCGGTGACCTTGCCAACAAACAAGCCCCTATAT‑GGGGCTTgtttcg                                                                                                                                        >  5:11190/1‑149 (MQ=255)
              cGTCAAGCTGCAATGCGCGGTGACGTACCAGGTCTGGTCAAGGCCTCCTGGTAATCGCTGCAGGCAAGGCGCGGGTGGAAGGGGAGCAATCTTCCGACC‑GCCGGTGACCTTGCCAACAAACAAGCCCCTATAT‑GGGGCTTgtttcgttt                                                                                                                                     <  6:11190/149‑1 (MQ=255)
              cGTCAAGCTGCAATGCGCGGTGACGTACCAGGTCTGGTCAAGGCCTCCTGGTAATCGCTGCAGGCAAGGCGCGGGTGGAAGGGGAGCAATCTTCCGACC‑GCCGGTGACCTTGCCAACAAACAAGCCCC‑ATATAGGGGCTTgtttgtt                                                                                                                                       <  3:62609/147‑4 (MQ=255)
                  aaGCTGCAATGCGCGGTGACGTACCAGGTCTGGTCAAGGCCTCCTGGTAATCGCTGCAGGCAAGGCGCGGGTGGAAGGGGAGCAATCTTCCGACC‑GCCGGTGACCTTGCCAACAAACAAGCCCCTATAT‑GGGGCTTgtttcgt                                                                                                                                       >  1:339928/1‑143 (MQ=255)
                  aaGCTGCAATGCGCGGTGACGTACCAGGTCTGGTCAAGGCCTCCTGGTAATCGCTGCAGGCAAGGCGCGGGTGGAAGGGGAGCAATCTTCCGACC‑GCCGGTGACCTTGCCAACAAACAAGCCCCTATAT‑GGGGCTTgtttcgt                                                                                                                                       <  2:339928/143‑1 (MQ=255)
                  aaGCTGCAATGCGCGGTGACGTACCAGGTCTGGTCAAGGCCTCCTGGTAATCGCTGCAGGCAAGGCGCGGGTGGAAGGGGAGCAATCTTCCGACC‑GCCGGTGACCTTGCCAACAAACAAGCCCCTATAT‑GGGGCTTGTTTCGTTTCTGc                                                                                                                                 <  6:218990/149‑1 (MQ=255)
                  aaGCTGCAATGCGCGGTGACGCACCAGGTCTGGTCAAGGCCTCCTGGTAATCGCTGCAGGCAAGGCGCGGGTGGAAGGGGAGCAATCTTCCGACC‑GCCGGTGACCTTGCCAACAAACAAGCCCCTATAT‑GGGGCTTgtttcgt                                                                                                                                       <  4:133272/143‑1 (MQ=255)
                  aaGCTGCAATGCGCGGTGACGAACCAGGTCTGGTCAAGGCCTCCTGGTAATCGCTGCAGGCAAGGCGCGGGTGGAAGGGGAGCAATCTTCCGACC‑GCCGGTGACCTTGCCAACAAACAAGCCCCTATAT‑GGGGCTTgtttcgt                                                                                                                                       >  3:133272/1‑143 (MQ=255)
                    gCTGCAATGCGCGGTGACGTACCAGGTCTGGTCAAGGCCTCCTGGTAATCGCTGCAGGCAAGGCGCGGGTGGAAGGGGAGCAATCTTCCGACC‑GCCGGTGACCTTGCCAACAAACAAGCCCCTATAT‑GGGGCTTGTTTCGTTTCTGCCt                                                                                                                               >  3:103393/1‑149 (MQ=255)
                     cTGCAATGCGCGGTGACGTACCAGGTCTGGTCAAGGCCTCCTGGTAATCGCTGCAGGCAAGGCGCGGGTGGAAGGGGAGCAATCTTCCGACC‑GCCGGTGACCTTGCCAACAAACAAGCCCCTATAT‑GGGGCTTGTTTCGTTTCTGCCtt                                                                                                                              <  6:142681/149‑1 (MQ=255)
                     cTGCAATGCGCGGTGACGTACCAGGTCTGGTCAAGGCCTCCTGGTAATCGCTGCAGGCAAGGCGCGGGTGGAAGGGGAGCAATCTTCCGACC‑GCCGGTGACCTTGCCAACAAACAAGCCCCTATAT‑GGGGCTTGTTTCGTTTCTGCCtt                                                                                                                              <  7:206703/149‑1 (MQ=255)
                       gCAATGCGCGGTGACGTACCAGGTCTGGTCAAGGCCTCCTGGTAATCGCTGCAGGCAAGGCGCGGGTGGAAGGGGAGCAATCTTCCGACC‑GCCGGTGACCTTGCCAACAAACAAGCCCCTATAT‑GGGGCTTGTTTCGTTTCTGc                                                                                                                                 >  5:317262/1‑144 (MQ=255)
                       gCAATGCGCGGTGACGTACCAGGTCTGGTCAAGGCCTCCTGGTAATCGCTGCAGGCAAGGCGCGGGTGGAAGGGGAGCAATCTTCCGACC‑GCCGGTGACCTTGCCAACAAACAAGCCCCTATAT‑GGGGCTTGTTTCGTTTCTGc                                                                                                                                 <  6:317262/144‑1 (MQ=255)
                       gCAATGCGCGGTGACGTACCAGGTCTGGTCAAGGCCTCCTGGTAATCGCTGCAGGCAAGGCGCGGGTGGAAGGGGAGCAATCTTCCGACC‑GCCGGTGACCTTGCCAACAAACAAGCCCCTATAT‑GGGGCTTGTTTCGTTTCTGCCTtgt                                                                                                                            >  2:304023/1‑149 (MQ=255)
                         aaTGCGCGGTGACGTACCAGGTCTGGTCAAGGCCTCCTGGTAATCGCTGCAGGCAAGGCGCGGGTGGAAGGGGAGCAATCTTCCGACC‑GCCGGTGACCTTGCCAACAAACAAGCCCCTATAT‑GGGGCTTGTTTCGTTTCTGCCTTgtgt                                                                                                                          <  7:68361/149‑1 (MQ=255)
                         aaTGCGCGGTGACGTACCAGGTCTGGTCAAGGCCTCCTGGTAATCGCTGCAGGCAAGGCGCGGGTGGAAGGGGAGCAATCTTCCGACC‑GCCGGTGACCTTGCCAACAAACAAGCCCCTATAT‑GGGGCTTGTTTCGTTTCTGCCTTgtgt                                                                                                                          >  5:263473/1‑149 (MQ=255)
                             cgcgGTGACGTACCAGGTCTGGTCAAGGCCTCCTGGTAATCGCTGCAGGCAAGGCGCGGGTGGAAGGGGAGCAATCTTCCGACC‑GCCGGTGACCTTGCCAACAAACAAGCCCCTATAT‑GGGGCTTGTTTCGTTTCTGCCTTGTGTCTa                                                                                                                       <  2:95693/148‑1 (MQ=255)
                             cgcgGTGACGTACCAGGTCTGGTCAAGGCCTCCTGGTAATCGCTGCAGGCAAGGCGCGGGTGGAAGGGGAGCAATCTTCCGACC‑GCCGGTGACCTTGCCAACAAACAAGCCCCTATAT‑GGGGCTTGTTTCGTTTCTGCCTTGTGTCTa                                                                                                                       <  3:179838/148‑1 (MQ=255)
                                         ccAGGTCTGGTCAAGGCCTCCTGGTAATCGCTGCAGGCAAGGCGCGGGTGGAAGGGGAGCAATCTTCCGACC‑GCCGGTGACCTTGCCAACAAACAAGCCCCTATAT‑GGGGCTTGTTTCGTTTCTGCCTTGTGTCTAGAA‑TGACCGGCt                                                                                                          >  3:143614/1‑148 (MQ=255)
                                             gTCTGGTCAAGGCCTCCTGGTAATCGCTGCAGGCAAGGCGCGGGTGGAAGGGGAGCAATCTTCCGACC‑GCCGGTGACCTTGCCAACAAACAAGCCCCTATAT‑GGGGCTTGTTTCGTTTCTGCCTTGTGTCTAGAA‑TGACCGGCTCACCt                                                                                                     <  2:319534/149‑1 (MQ=255)
                                               cTGGTCAAGGCCTCCTGGTAATCGCTGCAGGCAAGGCGCGGGTGGAAGGGGAGCAATCTTCCGACC‑GCCGGTGACCTTGCCAACAAACAAGCCCCTATAT‑GGGGCTTGTTTCGTTTCTGCCTTGTGTCTAGAA‑TGACCGGCTCAc                                                                                                       <  4:285257/145‑1 (MQ=255)
                                               cTGGTCAAGGCCTCCTGGTAATCGCTGCAGGCAAGGCGCGGGTGGAAGGGGAGCAATCTTCCGACC‑GCCGGTGACCTTGCCAACAAACAAGCCCCTATAT‑GGGGCTTGTTTCGTTTCTGCCTTGTGTCTAGAA‑TGACCGGCTCAc                                                                                                       >  3:285257/1‑145 (MQ=255)
                                                  gTCAAGGCCTCCTGGTAATCGCTGCAGGCAAGGCGCGGGTGGAAGGGGAGCAATCTTCCGACC‑GCCGGTGACCTTGCCAACAAACAAGCCCCTATAT‑GGGGCTTGTTTCGTTTCTGCCTTGTGTCTAGAA‑TGACCGGCTCACCTGAGc                                                                                                 <  1:304023/148‑1 (MQ=255)
                                                     aaGGCCTCCTGGTAATCGCTGCAGGCAAGGCGCGGGTGGAAGGGGAGCAATCTTCCGACC‑GCCGGTGACCTTGCCAACAAACAAGCCCCTATAT‑GGGGCTTGTTTCGTTTCTGCCTTGTGTCTAGAA‑TGACCGGCTCACCTGAGCCTgg                                                                                             <  4:153959/149‑1 (MQ=255)
                                                     aaGGCCTCCTGGTAATCGCTGCAGGCAAGGCGCGGGTGGAAGGGGAGCAATCTTCCGACC‑GCCGGTGACCTTGCCAACAAACAAGCCCCTATAT‑GGGGCTTGTTTCGTTTCTGCCTTGTGTCTAGAA‑TGACCGGCTCACCTGAGCCTgg                                                                                             <  4:103393/149‑1 (MQ=255)
                                                      aGGCCTCCTGGTAATCGCTGCAGGCAAGGCGCGGGTGGAAGGGGAGCAATCTTCCGACC‑GCCGGTGACCTTGCCAACAAACAAGCCCCTATAT‑GGGGCTAGTTTCGTTTCTGCCTTGTGTCTAGAA‑TGACCGGCTCACCTGAGCCTggg                                                                                            >  8:350913/1‑149 (MQ=255)
                                                       ggCCTCCTGGTAATCGCTGCAGGCAAGGCGCGGGTGGAAGGGGAGCAATCTTCCGACC‑GCCGGTGACCTTGCCAACAAACAAGCCCCTATAT‑GGGGCTTGTTTCGTTTCTGCCTTGTGTCTAGAA‑TGACCGGCTCACCTGAGCCTGGGt                                                                                           >  5:305257/1‑149 (MQ=255)
                                                          ctcctGGTAATCGCTGCAGGCAAGGCGCGGGTGGAAGGGGAGCAATCTTCCGACC‑GCCGGTGACCTTGCCAACAAACAAGCCCCTATAT‑GGGGCTTGTTTCGTTTCTGCCTTGTGTCTAGAATTGACCGGCTCACCTGAGCCTGGGttt                                                                                         <  1:3922/149‑1 (MQ=255)
                                                          ctcctGGTAATCGCTGCAGGCAAGGCGCGGGTGGAAGGGGAGCAATCTTCCGACC‑GCCGGTGACCTTGCCAACAAACAAGCCCCTATAT‑GGGGCTTGTTTCGTTTCTGCCTTGTGTCTAGAA‑TGACCGGCTCACCTGAGCCTGGGtttt                                                                                        >  5:310565/1‑149 (MQ=255)
                                                             ctGGCAATCTCTGCAGGACAGCCGCGGGTCCAACGGCAGCAATCATCCGACC‑GCCGGTGACCTTCCCAACAAACACGCCCCTATAT‑CCGGCTTGTTTCGTTTCTGCCTTGAGTCTAGAA‑TGACCGGCTCACCTGAGCCTGGGttttt                                                                                       <  6:258432/147‑1 (MQ=255)
                                                               ggTAATCGCTGCAGGCAAGGCGCGGGTGGAAGGGGAGCAATCTTCCGACCGGCCGGTGACCTTGCCAACAAACAAGCCCCTATAT‑GGGGCTTGTTTCGTTTCTGCCTTGTGTCTAGAA‑TGACCGGCTCACCTGAGCCTGGGTTTTTGcc                                                                                    >  1:278588/1‑149 (MQ=255)
                                                                gTAATCGCTGCAGGCAAGGCGCGGGTGGAAGGGGAGCAATCTTCCGACC‑GCCGGTGACCTTGCCAACAAACAAGCCCCTATAT‑GGGGCTTGTTTCGTTTCTGCCTTGTGTCTAGAA‑TGACCGGCTCACCTGAGCCTGGGTTTTTGccc                                                                                   <  6:263473/148‑1 (MQ=255)
                                                                       cTGCAGGCAAGGCGCGGGTGGAAGGGGAGCAATCTTCCGACC‑GCCGGTGACCTTGCCAACAAACAAGCCCCTATAT‑GGGGCTTGTTTCGTTTCTGCCTTGTGTCTAGAA‑TGACCGGCTCACCTGAGCCTGGGTTTTTGCCCAAACGGtt                                                                           >  4:298241/1‑149 (MQ=255)
                                                                        tGCAGGCAAGGCGCGGGTGGAAGGGGAGCAATCTTCCGACC‑GCCGGTGACCTTGCCAACAAACAAGCCCCTATAT‑GGGGCTTGTTTCGTTTCTGCCTTGTGTCTAGAA‑TGACCGGCTCACCTGAGCCTGGGTTTTTGCCCAAACGGTTc                                                                          >  4:106207/1‑149 (MQ=255)
                                                                          cAGGCAAGGCGCGGGTGGAAGGGTAGCAAGCTTCCGACC‑GCCGGTGACCTTGCCAACAAACACGCCCCTATAT‑GGGGCTTGTTTCGTTTCTGCCTTGTGTCTAGAA‑TGACCGGCTCACCTGAGCCTGGGTTTTTGCCCAAACGGTTCgg                                                                        <  4:213299/149‑1 (MQ=255)
                                                                          cAGGCAAGGCGCGGGTGGAAGGGGAGCAATCTTCCGACC‑GCCGGTGACCTTGCCAACAAACAAGCCCCTATAT‑GGGGCTTGTTTCGTTTCTGCCTTGTGTCTAGAA‑TGACCGGCTCACCTGAGCCTGGGTTTTTGCCCAAACGGTTc                                                                          <  5:67343/147‑1 (MQ=255)
                                                                          cAGGCAAGGCGCGGGTGGAAGGGGAGCAATCTTCCGACC‑GCCGGTGACCTTGCCAACAAACAAGCCCCTATAT‑GGGGCTTGTTTCGTTTCTGCCTTGTGTCTAGAA‑TGACCGGCTCACCTGAGCCTGGGTTTTTGCCCAAACGGTTc                                                                          <  6:305257/147‑1 (MQ=255)
                                                                          cAGGCAAGGCGCGGGTGGAAGGGGAGCAATCTTCCGACC‑GCCGGTGACCTTGCCAACAAACAAGCCCCTATAT‑GGGGCTTGTTTCGTTTCTGCCTTGTGTCTAGAA‑TGACCGGCTCACCTGAGCCTGGGTTTTTGCCCAAACGGTTCgg                                                                        <  5:379255/149‑1 (MQ=255)
                                                                            ggCAAGGCGCGGGTGGAAGGGGAGCAATCTTCCGACC‑GCCGGTGACCTTGCCAACAAACAAGCCCCTATAT‑GGGGCTTGTTTCGTTTCTGCCTTGTGTCTAGAA‑TGACCGGCTCACCTGAGCCTGGGTTTTTGCCCAAACGGTTCGggt                                                                      >  2:136320/1‑149 (MQ=255)
                                                                                   cgcgGGTGGAAGGGGAGCAATCTTCCGACC‑GCCGGTGACATTGCCAACAAACAAGCCCCTATAT‑GGGGCTTGTTTCGTTTCTGCCTTGTGTCTAGAA‑TGACCGGCTCACCTGAGCCTGGGTTTTTGCCCAAACGGTTCGGGTGGTTGt                                                                >  3:240844/1‑148 (MQ=255)
                                                                                     cgGGTGGAAGGGGAGCAATCTTCCGACC‑GCCGGTGACCTTGCCAACAAACAAGCCCCTATAT‑GGGGCTTGTTTCGTTTCTGCCTTGTGTCTAGAA‑TGACCGGCTCACCTGAGCCTGGGTTTTTGCCCAAACGGTTCGGGTGGTTGTCAt                                                             <  4:39872/149‑1 (MQ=255)
                                                                                            aaGGGGAGCAATCTTCCGACC‑GCCGGTGACCTTGCCAACAAACAAGCCCCTATAT‑GGGGCTTGTTTCGTTTCTGCCTTGTGTCTAGAA‑TGACCGGCTCACCTGAGCCTGGGTTTTTGCCCAAACGGTTCGGGTGGTTGTCATAGccttg                                                      >  2:77835/1‑146 (MQ=255)
                                                                                              ggggAGCAATCTTCCGACC‑GCCGGTGACCTTGCCAACAAACAAGCCCCTATAT‑GGGGCTTGTTTCGTTTCTGCCTTGTGTCTAGAA‑TGACCGGCTCACCTGAGCCTGGGTTTTTGCCCAAACGGTTCGGGTGGTTGTCATAGCCGCAAg                                                    <  3:106207/149‑1 (MQ=255)
                                                                                                    cAATCTTCCGACC‑GCCGGTGACCTTGCCAACAAACAAGCCCCTATAT‑GGGGCTTGTTTCGTTTCTGCCTTGTGTCTAGAA‑TGACCGGCTCACCTGAGCCTGGGTTTTTGCCCAAACGGTTCGGGTGGTTGTCATAGCCGCAAGGCtaa                                               >  4:143321/1‑148 (MQ=255)
                                                                                                      aTCTTCCGACC‑GCCGGTGACCTTGCCAACAAACAAGCCCCTATAT‑GGGGCTTGTTTCGTTTCTGCCTTGTGTCTAGAA‑TGACCGGCTCACCTGAGCCTGGGTTTTTGCCCAAACGGTTCGGGTGGTTGTCATAGCCGCAAGGCtaataa                                            >  5:56211/1‑149 (MQ=255)
                                                                                                       tCTTCCGACC‑GCCGGTGACCTTGCCAACAAACAAGCCCCTATAT‑GGGGCTTGTTTCGTTTCTGCCTTGTGTCTAGAA‑TGACCGGCTCACCTGAGCCTGGGTTTTTGCCCAAACGGTTCGGGTGGTTGTCATAGCCGCAAGGCTaataat                                           >  3:39686/1‑149 (MQ=255)
                                                                                                        cTTCCGACC‑GACGGTGACCTTGCCAACAAACAAGCCCCTATAT‑GGGGCTTGTTTCGTTTCTGCCTTGTGTCTAGAA‑TGACCGGCTCACCTGAGCATGGGTTTTTGACCAAACGGTTCGGGTGGTTGTCATAGACGCAAGGCAaataat                                           >  4:31719/1‑148 (MQ=255)
                                                                                                         ttCCGACC‑GCCGGTGACCTTGCCAACAAACAAGCCCCTATAT‑GGGGCTTGTTTCGTTTCTGCCTTGTGTCTAGAA‑TGACCGGCTCACCTGAGCCTGGGTTTTTGCCCAAACGGTTCGGGTGGTTGTCATAGCCGCAAGGCtagaatcg                                          >  7:121191/1‑142 (MQ=255)
                                                                                                           ccGACC‑GCCGGTGACCTTGCCAACAAACAAGCCCCTATAT‑TGGGCTTGTTTCGTTTCTGCCTTGTGTCTAGAA‑TGACCGGCTCACCTGAGCCTGGGTTTTTGCCCAAACGGTTCGGGTGGTTGTCATAGCCGCAAGGCTAATAATTCt                                        <  3:31719/148‑1 (MQ=255)
                                                                                                           ccGACC‑GCCGGTGACCTTGCCAACAAACAAGCCCCTATAT‑GGGGCTTGTTTCGTTTCTGCCTTGTGTCTAGAA‑TGACCGGCTCACCTGAGCCTgg                                                                                             >  6:17126/1‑95 (MQ=255)
                                                                                                           ccGACC‑GCCGGTGACCTTGCCAACAAACAAGCCCCTATAT‑GGGGCTTGTTTCGTTTCTGCCTTGTGTCTAGAA‑TGACCGGCTCACCTGAGCCTgg                                                                                             <  5:17126/95‑1 (MQ=255)
                                                                                                            cGACC‑GCCGGTGACCTTGCCAACAAACAAGCCCCTATAT‑GGGGCTTGTTTCGTTTCTGCCTTGTGTCTAGAA‑TGACCGGCTCACCTGAGCCTGGGTTTTTGCCCAAACGGTTCGGGTGGTTGTCATAGCCGCAAGGCTAATAATTCtt                                       <  2:278588/148‑1 (MQ=255)
                                                                                                             gACC‑GCCGGTGACCTTGCCAACAAACAAGCCCCTATAT‑GGGGCTTGTTTCGTTTCTGCCTTGTGTCTAGAA‑TGACCGACTCACCTGAGCATGGGTTTTTGCCAAAAAGGTTCGGGTGGTTGTCATAGACGCAAGGCTAATAATTCtag                                      >  3:267654/1‑146 (MQ=255)
                                                                                                             gACC‑GCAGGTGACCTTGCCAACAAACAAGCCCCTATAT‑GGGGCTTGTTTCGTTTCTGCCTTGTGTCTAGAA‑TGACCGGCTCACCTGAGCCTGGGTTTTTGCCCAAACGGTTCGGGTGGTTGTCATAGCCGCAAGGCTAATAATTCTTGt                                     <  4:143614/149‑1 (MQ=255)
                                                                                                             gACA‑GCCTGTGACCTTGCCAACAAACAAGCTCCTATAT‑TGGGCTTGTTTCGTTTCTGCCTTGTGTCTAGAA‑TGACCGGCTCACCTGAGCCTGGGTTTTTGCCCAAACGGTTCGGGTGGTTGTCATAGCCGAAAGGCTAATAATTc                                         <  4:267654/145‑1 (MQ=255)
                                                                                                              aCC‑GCCGGTGACCTTGCCAACAAACAAGCCCCTATAT‑GGGGCTTGTTTCGTTTCTGCCTTGTGTCTAGAA‑TGACCGGCTCACCTGAGCCTGGGTTTTTGCCCAAACGGTTCGGGTGGTTGTCATAGCCGCAAGGCTAATAATTc                                         <  5:142319/144‑1 (MQ=255)
                                                                                                              aCC‑GCCGGTGACCTTGCCAACAAACAAGCCCCTATAT‑GGGGCTTGTTTCGTTTCTGCCTTGTGTCTAGAA‑TGACCGGCTCACCTGAGCCTGGGTTTTTGCCCAAACGGTTCGGGTGGTTGTCATAGCCGCAAGGCTAATAATTc                                         >  6:142319/1‑144 (MQ=255)
                                                                                                                c‑gccgGTGACCTTGCCAACAAACAAGCCCCTATAT‑GGGGCTTGTTTCGTTTCTGCCTTGTGTCTAGAA‑TGACCGGCTCACCTGAGCCTGGGTTTTTGCCCAAACGGTTCGGGTGGTTGTCATAGCCGCAAGGCTAATAATTCTTGTATc                                  <  6:310565/149‑1 (MQ=255)
                                                                                                                   ccgGTGACCTTGCCAACAAACAAGCCCCTATAT‑GGGGCTTGTTTCGTTTCTGCCTTGTGTCTAGAA‑TGACCGGCTCACCTGAGCCTGGGTTTTTGCCCAAACGGTTCGGGTGGTTGTCATAGCCGCAAGGCTAATAATTCTTGt                                     <  8:10029/144‑1 (MQ=255)
                                                                                                                   ccgGTGACCTTGCCAACAAACAAGCCCCTATAT‑GGGGCTTGTTTCGTTTCTGCCTTGTGTCTAGAA‑TGACCGGCTCACCTGAGCCTGGGTTTTTGCCCAAACGGTTCGGGTGGTTGTCATAGCCGCAAGGCTAATAATTCTTGt                                     >  7:10029/1‑144 (MQ=255)
                                                                                                                   ccgGTGACCTTGCCAACAAACAAGCCCCTATAT‑GGGGCTTGTTTCGTTTCTGCCTTGTGTCTAGAA‑TGACCGGCTCACCTGAGCCTGGGTTTTTGCCCAAACGGTTCGGGTGGTTGTCATAGCCGCAAGGCTAATAATTCTTGTATCAg                                >  2:203548/1‑149 (MQ=255)
                                                                                                                         aCCTTGCCAACAAACAAGCCCCTATAT‑GGGGCTTGTTTCGTTTCTGCCTTGTGTCTAGAA‑TGACCGGCTCACCTGAGCCTGGGTTTTTGCCCAAACGGTTCGGGTGGTTGTCATAGCCGCAAGGCTAATAATTCTTGTATCAGGa                              <  5:286438/145‑1 (MQ=255)
                                                                                                                         aCCTTGCCAACAAACAAGCCCCTATAT‑GGGGCTTGTTTCGTTTCTGCCTTGTGTCTAGAA‑TGACCGGCTCACCTGAGCCTGGGTTTTTGCCCAAACGGTTCGGGTGGTTGTCATAGCCGCAAGGCTAATAATTCTTGTATCAGGa                              >  6:286438/1‑145 (MQ=255)
                                                                                                                            ttaccAACAAACAAGCCCCTATAT‑GGGGCTTGTTTCGTTTCTGCCTTGTGTCTAGAA‑TGACCGGCTCACCTGAGCCTGGGTTTTTGCCCAAACGGTTCGGGTGGTTGTCATAGCCGCAAGGCTAATAATTCTTg                                      >  4:184306/4‑134 (MQ=255)
                                                                                                                            ttaccAACAAACAAGCCCCTATAT‑GGGGCTTGTTTCGTTTCTGCCTTGTGTCTAGAA‑TGACCGGCTCACCTGAGCCTGGGTTTTTGCCCAAACGGTTCGGGTGGTTGTCATAGCCGCAAGGCTAATAATTCTTg                                      <  3:184306/131‑1 (MQ=255)
                                                                                                                              gCCAACAAACAAGCCCCTATAT‑GGGGCTTGTTTCGTTTCTGCCTTGTGTCTAGAA‑TGACCGGCTCACCTGAGCCTGGGTTTTTGCCCAAACGGTTCGGGTGGTTGTCATAGCCGCAAGGCTAATAATTCTTGTATCAGGAGCATCTAGc                     >  6:128126/1‑149 (MQ=255)
                                                                                                                               ccAACAAACAAGCCCCTATAT‑GGGGCTTGTTTCGTTTCTGCCTTGTGTCTAGAA‑TGACCGGCTCACCTGAGCCTGGGTTTTTGCCCAAACGGTTCGGGTGGTTGTCATAGCCGCAAGGCTAATAATTCTTGTATCAGGAGCATCTAGc                     <  3:298241/148‑1 (MQ=255)
                                                                                                                                caacaaACAAGCCCCTATAT‑GGGGCTTGTTTCGTTTCTGCCTTGTGTCTAGAA‑TGACCGGCTCACCTGAGCCTGGGTTTTTGCCCAAACGGTTCGGGTGGTTGTCATAGCCGCAAGGCTAATAATTCTTGTATCAGGAGCATc                         <  6:3411/143‑1 (MQ=255)
                                                                                                                                caacaaACAAGCCCCTATAT‑GGGGCTTGTTTCGTTTCTGCCTTGTGTCTAGAA‑TGACCGGCTCACCTGAGCCTGGGTTTTTGCCCAAACGGTTCGGGTGGTTGTCATAGCCGCAAGGCTAATAATTCTTGTATCAGGAGCATc                         >  5:3411/1‑143 (MQ=255)
                                                                                                                                caacaaACAAGCCCCTATAT‑GGGGCTTGTTTCGTTTCTGCCTTGTGTCTAGAA‑TGACCGGCTCACCTGAGCCTGGGTTTTTGCCCAAACGGTTCGGGTGGTTGTCATAGCCGCAAGGCTAATAATTCTTGTATCAGGAGCATCTAGcc                    >  4:117850/1‑148 (MQ=255)
                                                                                                                                caacaaACAAGCCCCTATAT‑GGGGCTTGTTTCGTTTCTGCCTTGTGTCTAGAA‑TGACCGGCTCACCTGAGCCTGGGTTTTTGCCCAAACGGTTCGGGTGGTTGTCATAGCCGCAAGGCTAATAATTCTTGTATCAGGAGCATCTAGcc                    >  6:333088/1‑148 (MQ=255)
                                                                                                                                caacaaACAAGCCCCTATAT‑GGGGCTTGTTTCGTTTCTGCCTTATGTCTAGAA‑TGACCGGCTCACCTGAGCCTGGGTTTTTGCCCAAACGGTTCGGGTGGTTGTCATAGCCGCAAGGCTAATAATTCTTGTATCAGGAGCATCTAGcc                    >  4:160245/1‑148 (MQ=255)
                                                                                                                                 aacaaacaAGCCCCTATAT‑GGGGCTTGTTTCGTTTCTGCCTTGTGTCTAGAA‑TGACCGGCTCACCTGAGCCTGGGTTTTTGCCCAAACGGTTCGGGTGGTTGTCATAGCCGCAAGGCTAATAATTCTTGTATCAGGAGCATCTAGCCCa                  <  6:56211/149‑1 (MQ=255)
                                                                                                                                 aacaaacaAGCCCCTATAT‑GGGGCTTGTTTCGTTTCTGCCTTGTGTCTAGAA‑TGACCGGCTCACCTGAGCCTGGGTTTTTGCCCAAACGGTTCGGGTGGTTGTCATAGCCGCAAGGCTAATAATTCTTGTATCAGGAGCATCTAGCCCa                  >  4:140561/1‑149 (MQ=255)
                                                                                                                                 aacaaacaAGCCCCTATAT‑GGGGCTTGTTTCGTTTCTGCCTTGTGTCTAGAA‑TGACCGGCTCACCTGAGCCTGGGTTTTTGCCCAAACGGTTCGGGTGGTTGTCATAGCCGCAAGGCTAATAATTCTTGTATCAGGAGCATCTAGCCCa                  >  3:290719/1‑149 (MQ=255)
                                                                                                                                  acaaacaaGCCCCTATAT‑GGGGCTTGTTTCGTTTCTGCCTTGTGTCTAGAA‑TGACCGGCTCACCTGAGCCTGGGTTTTTGCCCAAACGGTTCGGGTGGTTGTCATAGCCGCAAGGCTAATAATTCTTGTATCAGGAGCATCTAGCCCAt                 <  1:136320/149‑1 (MQ=255)
                                                                                                                                   gaaacaaGCCCC‑ATATAGGGGCTTGTTTCGTTTCTGCCTTGTGTCTAGAA‑TGACCGGCTCACCTGAGCCTGGGTTTTTGCCCAAACGGTTCGGGTGGTTGTCATAGCCGCAAGGCTAATAATTCTTGTATCAgg                               <  1:149637/133‑1 (MQ=255)
                                                                                                                                   gaaacaaGCCCC‑ATATAGGGGCTTGTTTCGTTTCTGCCTTGTGTCTAGAA‑TGACCGGCTCACCTGAGCCTGGGTTTTTGCCCAAACGGTTCGGGTGGTTGTCATAGCCGCAAGGCTAATAATTCTTGTATCAgg                               >  2:155128/2‑134 (MQ=255)
                                                                                                                                   gaaacaaGCCCC‑ATATAGGGGCTTGTTTCGTTTCTGCCTTGTGTCTAGAA‑TGACCGGCTCACCTGAGCCTGGGTTTTTGCCCAAACGGTTCGGGTGGTTGTCATAGCCGCAAGGCTAATAATTCTTGTATCAgg                               >  2:149637/2‑134 (MQ=255)
                                                                                                                                   gaaacaaGCCCC‑ATATAGGGGCTTGTTTCGTTTCTGCCTTGTGTCTAGAA‑TGACCGGCTCACCTGAGCCTGGGTTTTTGCCCAAACGGTTCGGGTGGTTGTCATAGCCGCAAGGCTAATAATTCTTGTATCAgg                               <  1:155128/133‑1 (MQ=255)
                                                                                                                                   caaacaaGCCCCTATAT‑GGGGCTTGTTTCGTTTCTGCCTTGTGTCTAGAA‑TGACCGGCTCACCTGAGCCTGGGTTTTTGCCCAAACGGTTCGGGTGGTTGTCATAGCCGCAAGGCTAATAATTCTTGTATCAGGAGCATCTAGCCCATg                <  3:143321/149‑1 (MQ=255)
                                                                                                                                    aaacaaGCCCCTATAT‑GGGGCTTGTTTCGTTTCTGCCTTCTGTCTAGAA‑TGACCGGCTCACCTGAGCCTGGGTTTTTGCCCAAACGGTTCGGGTGGTTGTCATAGCCGCAAGGCTAATAATTCTTGTATCAGGAGCATCTAGCCCATGa               <  3:160245/149‑1 (MQ=255)
                                                                                                                                     aacaaGCCCC‑ATATAGGGGCTTGTTTCGTTTCTGCCTTGTGTCTAGAA‑TGACCGGCTCACCTAAGCCTGGGTTTTTGCCCAAACGGTTCGGGTGGTTGTCATAGCCGCAAGGCTAATAATTCTTGTATCAGGAGCATCTAGCCCATGAg              >  6:212839/1‑149 (MQ=255)
                                                                                                                                      acaaGCCCCTATAT‑GGGGCTTGTTTCGTTTCTGCCTTGTGTCTAGAA‑TGACCGGCTCACCTGAGCCTGGGTTTTTGCCCTAACGGTTCGGGTGGTTGTCTTAGCCGCAAGGCTAATAATTCTTGTATCAGGAGCATCTAGCCCATGAGt             <  4:240844/149‑1 (MQ=255)
                                                                                                                                        aaGCCCCTATAT‑GGGGCTTGTTTCGTTTCTGCCTTGTGTCTAGAA‑TGACCGGCTCACCTGAGCCTGGGTTTTTGCCCAAACGGTTCGGGTGGTTGTCATAGCCGCAAGGCTAATAATTCTTGTATCAGGAGCATCTAGCCCATGAGTAt           >  4:321875/1‑149 (MQ=255)
                                                                                                                                          gCCCCTATAT‑GGGGCTTGTTTCGTTTCTGCCTTGTGTCTAGAA‑TGACCGGCTCACCTGAGCCTGGGTTTTTGCCCAAACGGTTCGGGTGGTTGTCATAGCCGCAAGGCTAATAATTCTTGTATCAGGAGCATCTAGCCCATGAGTATGc         <  7:350913/149‑1 (MQ=255)
                                                                                                                                           ccccTATAT‑GGGGCTTGTTTCGTTTCTGCCTTGTGTCTAGAA‑TGACCGGCTCACCTGAGCCTGGGTTTTTGCCCAAACGGTTCGGGTGGTTGTCATAGCCGCAAGGCTAATAATTCTTGTATCAGGAGCATCTAGCCCATGAGTATg          >  7:97941/1‑147 (MQ=255)
                                                                                                                                            cccTATAT‑GGGGCTTGTTTCGTTTCTGCCTTGTGTCTAGAA‑TGACCGGCTCACCTGAGCCTGGGTTTTTGCCCAAACGGTTCGGGTGGTTGTCATAGCCGCAAGGCTAATAATTCTTGTATCAGGAGCATCTAGCCCATGAGTATGCAg       >  4:293877/1‑149 (MQ=255)
                                                                                                                                              cTATAT‑GGGGCTTGTTTCGTTTCTGCCTTGTGTCTAGAA‑TGACCGGCTCACCTGAGCCTGGGTTTTTGCCCAAACGGTTCGGGTGGTTGTCATAGCCGCAAGGCTAATAATTCTTGTATCAGGAGCATCTAGCCCATGAGTATGCAGGa     <  4:39686/149‑1 (MQ=255)
                                                                                                                                                 tat‑GGGGCTTGTTTCGTTTCTGCCTTGTGTCTAGAA‑TGACCGGCTCACCTGAGCCTGGGTTTTTGCCCAAACGGTTCGGGTGGTTGTCATAGCCGCAAGGCTAATAATTCTTGTATCAGGAGCATCTAGCCCATGAGTATGCAGGAccc  <  8:97941/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
GCCGTAACATGCTGCGTCAAGCTGCAATGCGCGGTGACGTACCAGGTCTGGTCAAGGCCTCCTGGTAATCGCTGCAGGCAAGGCGCGGGTGGAAGGGGAGCAATCTTCCGACC‑GCCGGTGACCTTGCCAACAAACAAGCCCCTATAT‑GGGGCTTGTTTCGTTTCTGCCTTGTGTCTAGAA‑TGACCGGCTCACCTGAGCCTGGGTTTTTGCCCAAACGGTTCGGGTGGTTGTCATAGCCGCAAGGCTAATAATTCTTGTATCAGGAGCATCTAGCCCATGAGTATGCAGGACCC  >  NC_002947/557190‑557482

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑