| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 1,126,645:1 | +C | 100% | intergenic (‑99/+50) | tdcG‑II ← / ← gcvP‑I | L‑serine dehydratase/glycine dehydrogenase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 1,126,645 | 1 | . | C | 96.2% | 81.7 / ‑2.7 | 26 | intergenic (‑99/+50) | tdcG‑II/gcvP‑I | L‑serine dehydratase/glycine dehydrogenase |
| Reads supporting (aligned to +/- strand): ref base . (1/0); new base C (13/12); total (14/12) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.26e-01 | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
GGGCCGATGCCGATCTTGAACAGGTCGAAGACGCTCAGTGACATGTGGACCTCCTGCCAGGTTGTTGGGTGGACATTGAGCCCTGTAGGCGCGGG‑TTTACCCGCGAATGCGGAGGTATAGCCACCATCGCATTCGCGGGT‑AAA‑CCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGTCGCCGAACACGTTGTCGACCCGGCCGACCGGCGGCCAGTA > NC_002947/1126503‑1126787 | gggCCGATGCCGATCTTGAACAGGTCGAAGACGCTCAGTGACATGTGGACCTCCTGCCAGGTTGTTGGGTGGACATTGAGCCCTGTAGGCGCGGG‑TTTACCCGCGAATGCGGAGGTATAGCCACCATCGCATTCGCGGGT‑AAA‑cccgct < 3:1045/149‑5 (MQ=255) ggCCGATGCCGATCTTGAACAGGTCGAAGACGCTCAGTGACATGTGGACCTCCTGCCAGGTTGTTGGGTGGACATTGAGCCCTGTAGGCGCGGG‑TTTACCCGCGAATGCGGAGGTATAGCCACCATCGCATTCGCGGGT‑AAA‑cccgctc < 4:315837/149‑6 (MQ=255) tgcctatCTTTAACAGGGCTAAGAGGCTCTGTTACTTGTGCACCTCCTGCCTGGTTGTTGGGTGTACTTTGAGCCCTGTCGGCGGGGG‑TTTACCCGCTATTGCGGTGTTATAGCCACCATCGCATTCGGGGTA‑AAACCCGCTCCTaca < 8:128508/148‑1 (MQ=255) gAACAGGTCGAAGACGCTCAGTGACATGTGGACCTCCTGCCAGGTTGTTGGGTGGACATTGAGCCCTGTAGGCGCGGG‑TTTACCCGCGAATGCGGAGGTATAGCCACCATCGCATTCGCGGGT‑AAACCCGCTCCTACACAGAGCCCGc < 5:79532/148‑1 (MQ=255) aaGACGCTCAGTGACATGTGGACCTCCTGCCAGGTTGTTGGGTGGACATTGAGCCCTGTAGGCGCGGG‑TTTACCCGCGAATGCGGAGGTATAGCCACCATCGCATTCGGGGGT‑AAACCCGCTCCTACACAGAGCCCGCCACCCAAGCCa < 2:89523/149‑1 (MQ=255) gcAGTCACTTTTGTACCTCCTGCCTGGTTGTTGGGGGTACTTTCAGCCCTGTAGGCGGGGG‑TTTACCCGCTAATGCGGAGGTATAGCCACCATCCCTTTCGCGGGT‑AAACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCg < 8:51776/148‑1 (MQ=25) gACATCCTGCCTGTTTGTTGGGTGTACATTGAGCCCTGTTGGCGGGGG‑TTTACCCGGTAATGCTGAGGTATAGCCACCATCTCTTTGGGGGTT‑AAACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATc < 6:26139/148‑1 (MQ=255) gttgttGGGGGGACTTTGAGCCGTGTAGGCGCGGG‑TTTACCCGCTAATGGGGAGGTATTGCCACAATCGCATTCGCGGGT‑AAACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATa < 4:241500/148‑1 (MQ=37) cATTGAGCCCTGTAGGCGCGGG‑TTTAACCCCCAAAGAGGAGGTATAAACACCAAAGCATAACCGGGT‑AAACCCCCTCCAACACAGAGCCCCCCACCCCACCCACGTGGGGGGGAGGGCAATAAAGCGTCCAGATAACTAAAGATCGACg > 2:119236/1‑149 (MQ=255) aTTGAGCCCTGTAGGCGCGGGTTTTAACCCCAAAT‑CGGAGGTATTACCAACAAAGCAATAGCCGGTAAAA‑CCCCGCCGACACAAAGCCCCCCCCCCCAGCCACGGGGGGGGGGGGGCGATAAAGCGGAGTGATAGATCACGATAGAccg > 4:106689/1‑147 (MQ=255) gcgGG‑TTTACCCGCGAATGCGGAGGTATAGCCACCATCGCATTCGCGGGT‑AAACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACg < 5:365893/132‑1 (MQ=255) gcgGG‑TTTACCCGCGAATGCGGAGGTATAGCCACCATCGCATTCGCGGGT‑AAACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACg > 6:365893/1‑132 (MQ=255) tACCCGCGAATGCGGAGGTATAGCCACCATCGCATTCGCGGGT‑AAACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGt > 2:127826/1‑149 (MQ=255) ccGCGAATGCGGAGGTATAGCCACCATCGCATTCGCGGGT‑AAACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGTCGc > 4:163673/1‑149 (MQ=255) gcgAATGCGGAGGTATAGCCACCATCGCATTCGCGGGT‑AAACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGTCGcc > 6:362768/1‑148 (MQ=255) gcgAATGCGGAGGTATAGCCACCATCGCATTCGCGGGT‑AAACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGTCGcc > 7:123731/1‑148 (MQ=255) gTATAGCCACCATCGCATTCGCGGGT‑AAACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGa > 4:288811/1‑92 (MQ=255) gTATAGCCACCATCGCATTCGCGGGT‑AAACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGa < 3:288811/92‑1 (MQ=255) gCCACCATCGCATTCGCGGGT‑AAACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGTCGCCGAACACGTTGTCGAccc < 8:123731/148‑1 (MQ=255) gCCACCATCGCATTCGCGGGT‑AAACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGTCGCCGAACACGTTGTCGACCCg > 8:341599/1‑149 (MQ=255) caccaTCGCATTCGCGGGT‑AAACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGTCGCCGAACACGTTGTCGACCCGGc > 3:319142/1‑149 (MQ=255) caccaTCGCATTCGCGGGT‑AAACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGTCGCCGAACACGTTGTCGACCCGGc > 8:254863/1‑149 (MQ=255) caccaTCGCATTCGCGGGT‑AAACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGTCGCCGAACACGTTGTCGACCCGGc > 8:313653/1‑149 (MQ=255) aTCGCATTCGCGGGT‑AAACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGTCGCCGAACACGTTGTCGACCCGGCCGAc < 3:163673/149‑1 (MQ=255) cATTCGCGGGT‑AAACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGTCGCCGAACACGTTg > 7:37739/1‑131 (MQ=255) cATTCGCGGGT‑AAACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGTCGCCGAACACGTTg < 8:37739/131‑1 (MQ=255) cgcgGGT‑AAACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGTCGCCGAACACGTTGTCGACCCGGCCGACCggcggc < 3:106689/148‑1 (MQ=255) gT‑AAACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGTCGCCGAACACGTTGTCGACCCGGCCGACCGGCGGCCAGTa > 4:271441/1‑148 (MQ=255) | GGGCCGATGCCGATCTTGAACAGGTCGAAGACGCTCAGTGACATGTGGACCTCCTGCCAGGTTGTTGGGTGGACATTGAGCCCTGTAGGCGCGGG‑TTTACCCGCGAATGCGGAGGTATAGCCACCATCGCATTCGCGGGT‑AAA‑CCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGTCGCCGAACACGTTGTCGACCCGGCCGACCGGCGGCCAGTA > NC_002947/1126503‑1126787 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |