Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,707,288:1 +A 7.3% intergenic (+106/+36) PP_1501 → / ← PP_1502 hypothetical protein/OmpA family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,707,2881.A 7.3% 217.7 / 10.4 55intergenic (+106/+36)PP_1501/PP_1502hypothetical protein/OmpA family protein
Reads supporting (aligned to +/- strand):  ref base . (21/30);  new base A (2/2);  total (23/32)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.39e-01

CATGGGCGAGGAAACACCGCGGCAGGTGCAGTGAGGTTTCGGCGCAGCATTTTTGGCGCCCGCGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGGCACAACATTATCGGCGAACAAAGAAAAGCCCCGCG‑TCGATCGCGGGGCTTGTCCTTTCAGCTAGCCAGCCTTAGCGCTGAACCTGGCTCACCGAGCGTGGTGCCACCGGCTGGTTGTCGTTGGAGATGGTCACCTCCACCCGACGGTTCTGCGCACGTCCCGAGTTGCTCGAGTTGT  >  NC_002947/1707149‑1707430
                                                                                                                                            |                                                                                                                                              
cATGGGCGAGGAAACACCGCGGCAGGTGCAGTGAGGTTTCGGCGCAGCATTTTTGGCGCCCGCGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGGCACAACATTATCGGCGAACAAAGAAAAGCCCCGCG‑TCGATcgc                                                                                                                                        <  7:214464/148‑1 (MQ=255)
  tGGGCGAGGAAACACCGCGGCAGGTGCAGTGAGGTTTCGGCGCAGCATTTTTGGCGCCCGCGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGGCACAACATTATCGGCGAACAAAGAAAAGCCCCGCG‑TCGATCGCggg                                                                                                                                     <  3:278822/149‑1 (MQ=255)
         ggAAACACCGCGGCAGGTGCAGTGAGGTTTCGGCGCAGCATTTTTGGCGCCCGCGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGGCACAACATTATCGGCGAACAAAGAAAAGCCCCGCGATCGA‑CGCGGGGCTTTTc                                                                                                                              >  5:193404/1‑149 (MQ=255)
         ggAAACACCGCGGCAGGTGCAGTGAGGTTTCGGCGCAGCATTTTTGGCGCCCGCGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGGCACAACATTATCGGCGAACAAAGAAAAGCCCCGCG‑TCGATCGCGGGGCTTGTc                                                                                                                              >  2:222618/1‑149 (MQ=255)
          gAAACACCGCGGCAGGTGCAGTGAGGTTTCGGCGCAGCATTTTTGGCGCCCGCGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGGCACAACATTATCGGCGAACAAAGAAAAGCCCCGCGATCGA‑CGCGGGGCTTTTct                                                                                                                             <  2:42815/149‑2 (MQ=39)
          gAAACACCGCGGCAGGTGCAGTGAGGTTTCGGCGCAGCATTTTTGGCGCCCGCGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGGCACAACATTATCGGCGAACAAAGAAAAGCCCCGCG‑TCGATCGCGGGGCTTGTcc                                                                                                                             >  2:212112/1‑149 (MQ=255)
           aaaCACCGCGGCAGGTGCAGTGAGGTTTCGGCGCAGCATTTTTGGCGCCCGCGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGGCACAACATTATCGGCGAACAAAGAAAAGCCCCGCGATCGA‑CGCGGGGCTTTTctt                                                                                                                            <  6:193404/149‑3 (MQ=39)
              cacCGCGGCAGGTGCAGTGAGGTTTCGGCGCAGCATTTTTGGCGCCCGCGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGGCACAACATTATCGGCGAACAAAGAAAAGCCCCGCG‑TCGATCGCGGGGCTTGTCCTTTc                                                                                                                         <  4:302103/149‑1 (MQ=255)
               acCGCGGCAGGTGCAGTGAGGTTTCGGCGCAGCATTTTTGGCGCCCGCGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGGCACAACATTATCGGCGAACAAAGAAAAGCCCCGCG‑TCGATCGCGGGGCTTGTCCttt                                                                                                                          >  8:167911/1‑147 (MQ=255)
               acCGCGGCAGGTGCAGTGAGGTTTCGGCGCAGCATTTTTGGCGCCCGCGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGGCACAACATTATCGGCGAACAAAGAAAAGCCCCGCG‑TCGATCGCGGGGCTTGTCCTTTCa                                                                                                                        <  2:149474/149‑1 (MQ=255)
                ccGCGGCAGGTGCAGTGAGGTTTCGGCGCAGCATTTTTGGCGCCCGCGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGGCACAACATTATCGGCGAACAAAGAAAAGCCCCGCG‑TCGATCGCGGGGCTTGTCCTTTCAg                                                                                                                       <  4:285918/149‑1 (MQ=255)
                         gTGCAGTGAGGTTTCGGCGCAGCATTTTTGGCGCCCGCGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGGCACAACATTATCGGCGAACAAAGAAAAGCCCCGCG‑TCGATCGCGGGGCTTGTCCTTTCAGCTagccagc                                                                                                              >  6:290119/1‑149 (MQ=255)
                               tGAGGTTTCGGCGCAGCATTTTTGGCGCCCGCGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGGCACAACATTATCGGCGAACAAAGAAAAGCCCCGCG‑TCGATCGCGGGGCTTGTCCTTTCAGCTAGCCAGCCTTAgc                                                                                                        >  1:298754/1‑149 (MQ=255)
                                   gTTTCGGCGCAGCATTTTTGGCGCCCGCGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGGCACAACATTATCGGCGAACAAAGAAAAGCCCCGCG‑TCGATCGCGGGGCTTGTCCTTTCAGCTAGCCAGCCTTAGCGCTg                                                                                                    <  5:47662/149‑1 (MQ=255)
                                    tttCGGCGCAGCATTTTTGGCGCCCGCGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGGCACAACATTATCGGCGAACAAAGAAAAGCCCCGCG‑TCGATCGCGGGGCTTGTCCTTTCAGCTAGCCAGCCTTAGCGCTGa                                                                                                   <  2:160364/149‑1 (MQ=255)
                                        ggCGCAGCATTTTTGGCGCCCGCGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGGCACAACATTATCGGCGAACAAAGAAAAGCCCCGCG‑TCGATCGCGGGGCTTGTCCTTTCAGCTAGCCAGCCTTAGCGCTGAACCt                                                                                               >  8:123610/1‑149 (MQ=255)
                                           gcagcaTTTTTGGCGCCCGCGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGGCACAACATTATCGGCGAACAAAGAAAAGCCCCGCG‑TCGATCGCGGGGCTTGTCCTTTCAGCTAGCCAGCCTTAGCGCTGAACCTgg                                                                                             <  5:203117/148‑1 (MQ=255)
                                           gcagcaTTTTTGGCGCCCGCGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGGCACAACATTATCGGCGAACAAAGAAAAGCCCCGCG‑TCGATCGCGGGGCTAGTCCTTTCAGCTAGCCAGCCTTAGCGCTGAACCTgg                                                                                             >  6:203117/1‑148 (MQ=255)
                                                       gcgcCCGCGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGGCACAACATTATCGGCGAACAAAGAAAAGCCCCGCG‑TCGATCGCGGGGCTTGTCCTTTCAGCTAGCCAGCCTTAGCGCTGAACCTGGCTCACCGAGCgtg                                                                                <  6:12385/149‑1 (MQ=255)
                                                         gcCCGCGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGGCACAACATTATCGGCGAACAAAGAAAAGCCCCGCG‑TCGATCGCGGGGCTTGTCCTTTCAGCTAGCCAGCCTTAGCGCTGAACCTGGCTCACCGAGCgtggt                                                                              <  5:210083/149‑1 (MQ=255)
                                                              cgAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGGCACAACATTATCGGCGAACAAAGAAAAGCCCCGCG‑ACGATCGCGGGGCTTGTCCTTTCACCTAGCCAGCCTTAGCCCTGAACCAGGCTCACCGAGCCTGGTGCCAc                                                                         >  2:336910/1‑149 (MQ=39)
                                                                agaTCGAGCGCCGCCCGCGCGGCGCTCGATCTCACAGGCGGCACAACATTATCGGCGAACAAAGAAAAGCCCCGCG‑TCGATCGCGGGGCTTGTCCTTTCAGCTAGCCAGCCTTAGCGCTGAACCTGGCTCACCGAGCGTGGTGCCACCg                                                                       >  8:239377/1‑149 (MQ=255)
                                                                      aGCGCCGCCCGCGCGGCGCTCGATCTCACAGGCGGCACAACATTATCGGCGAACAAAGAAAAGCCCCGCG‑TCGATCGCGGGGCTTGTCCTTTCAGCTAGCCAGCCTTAGCGCTGAACCTGGCTCACCGAGCGTGGTGCCACCGGCTGGt                                                                 >  2:77334/1‑149 (MQ=255)
                                                                        cgccgcGCGGGCGGCGCTCGATCTCACAGGCGGCACAACATTATCGGCGAACAAAGAAAAGCCCCGCG‑TCGATCGCGGGGCTTGTCCTTTCAGCTAGCCAGCCTTAGCGCTGAACCTGGCTCACCGAGCGTGGTGCCACCGGCTGGTTg                                                               >  5:98930/1‑149 (MQ=255)
                                                                         gccgcGCGGGCGGCGCTCGATCTCACAGGCGGCACAACATTATCGGCGAACAAAGAAAAGCCCCGCG‑TCGATCGCGGGGCTTGTCCTTTCAGCTAGCCAGCCTTAGCGCTGAACCTGGCTCACCGAGCGTGGTGCCACCGGCTGGTTGt                                                              >  5:219362/1‑149 (MQ=255)
                                                                          ccgcGCGGGCGGCGCTCGATCTCACAGGCGGCACAACATTATCGGCGAACAAAGAAAAGCCCCGCG‑TCGATCGCGGGGCTTGTCCTTTCAGCTAGCCAGCCTTAGCGCTGAACCTGGCTCACCGAGCGTGGTGCCACCGGCTGGTTGTc                                                             >  3:77064/1‑149 (MQ=255)
                                                                          ccgcGCGGGCGGCGCTCGATCTCACAGGCGGCACAACATTATCGGCGAACAAAGAAAAGCCCCGCG‑TCGATCGCGGGGCTTGTCCTTTCAGCTAGCCAGCCTTAGCGCTGAACCTGGCTCACCGAGCGTGGTGCCACCGGCTGGTTGTc                                                             <  1:212112/149‑1 (MQ=255)
                                                                           cgcgcgGGCGGCGCTCGATCTCACAGGCGGCACAACATTATCGGCGAACAAAGAAAAGCCCCGCG‑TCGATCGCGGGGCTTGTCCTTTCAGCTAGACAGCATTAGCGCTGAACCTGGCTCACCGAGCGTGGTGCCACCGCCTCGTTGTCg                                                            >  7:12152/1‑149 (MQ=255)
                                                                             cgcgGGCGGCGCTCGATCTCACAGGCGGCACAACATTATCGGCGAACAAAGAAAAGCCCCGCG‑TCGATCGCGGGGCTTGTCCTTTCAGCTAGCCAGCCTTAGCGCTGAACCTGGCTCACCGAGCGTGGTGCCACCGGCTGGTTGTCGtt                                                          <  7:167911/149‑1 (MQ=255)
                                                                              gcgggcggCGCTCGATCTCACAGGCGGCACAACATTATCGGCGAACAAAGAAAAGCCCCGCG‑TCGATCGCGGGGCTTGTCCTTTCAGCTAGCCAGCCTTAGCGCTGAACCTGGCTCACCGAGCGTGGTGCCACCGGCTGGTTGTCGTTg                                                         <  4:77064/149‑1 (MQ=255)
                                                                              gcgggcggCGCTCGATCTCACAGGCGGCACAACATTATCGGCGAACAAAGAAAAGCCCCGCG‑TCGATCGCGGGGCTTGTCCTTTCAGCTAGCCAGCCTTAGCGCTGAACCTGGCTCACCGAGCGTGGTGCCACCGGCTGGTTGTCGTTg                                                         <  5:290119/149‑1 (MQ=255)
                                                                              gcgggcggCGCTCGATCTCACAGGCGGCACAACATTATCGGCGAACAAAGAAAAGCCCCGCG‑TCGATCGCGGGGCTTGTCCTTTCAGCTAGCCAGCCTTAGCGCTGAACCTGGCTCACCGAGCGTGGTGCCACCGGCTGGTTGTCGTTg                                                         <  6:207722/149‑1 (MQ=255)
                                                                               cgcgcggcgCTCGATCTCACAGGCGGCACAACATTATCGGCGAACAAAGAAAAGCCCCGCG‑TCGATCGCGGGGCTTGTCCTTTCAGCTAGCCAGCCTTAGCGCTGAACCTGGCTCACCGAGCGTGGTGCCACCGGCTGGTTGTCGTTgg                                                        <  1:77334/146‑1 (MQ=255)
                                                                                gggcggCGCTCGATCTCACAGGCGGCACAACATTATCGGCGAACAAAGAAAAGCCCCGCG‑TCGATCGCGGGGCTTGTCCTTTCAGCTAGCCAGCCTTAGCGCTGAACCTGGCTCACCGAGCGTGGTGCCACCGGCTGGTTGTCGTTGga                                                       <  5:225408/149‑1 (MQ=255)
                                                                                gcgcggcgCTCGATCTCACAGGCGGCACAACATTATCGGCGAACAAAGAAAAGCCCCGCG‑TCGATCGCGGGGCTTGTCCTTTCAGCTAGCCAGCCTTAGCGCTGAACCTGGCTCACCGAGCGTGGTGCCACCGGCTGGTTGTCGTTGga                                                       <  7:239377/147‑1 (MQ=255)
                                                                                      cgcTCGATCTCTCAGGCGGCACAACATTATCGGCGAACAAAGAAAAGCCCCGCG‑TCGATCGCGGGGCTTGTCCTTTCAGCTAGCCAGCCTTAGCGCTGAACCTGGCTCACGGAGCGTGGTGCCACCGGCTGGTTGTCGTTGGAGATGGt                                                 <  1:336910/149‑1 (MQ=255)
                                                                                        cTCGATCTCACAGGCGGCACAACATTATCGGCGAACAAAGAAAAGCCCCGCG‑TCGATCGCGGGGCTTGTCCTTTCAGCTAGCCAGCCTTAGCGCTGAACCTGGCTCACCGAGCGTGGTGCCACCGGCTGGTTGTCGTTGGAGATGGTCa                                               <  7:123610/149‑1 (MQ=255)
                                                                                           gATCTCACGGGCGGCACAACATTATCGGCGAACAAAGAAAAGCCCCGCG‑TCGATCGCGGGGCTTGTCCTTTCAGCTAGCCAGCCTTAGCGCTGAACCTGGCTCACCGAGCgtg                                                                                >  5:325648/1‑113 (MQ=255)
                                                                                           gATCTCACGGGCGGCACAACATTATCGGCGAACAAAGAAAAGCCCCGCG‑TCGATCGCGGGGCTTGTCCTTTCAGCTAGCCAGCCTTAGCGCTGAACCTGGCTCACCGAGCgtg                                                                                <  6:325648/113‑1 (MQ=255)
                                                                                           gATCTCACAGGCGGCACAACATTATCGGCGAACAAAGAAAAGCCCCGCG‑TCGATCGCGGGGCTTGTCCTTTCAGCTAGCCAGCCTTAGCGCTGAACCTGGCTCACCGAGCGTGGTGCCACCGGCTGGTTGTCGTTGGAGATGGTCAc                                              <  2:298754/147‑1 (MQ=255)
                                                                                               tcACAGGCGGCACAACATTATCGGCGAACAAAGAAAAGCCCCGCG‑TCGATCGCGGGGCTTGTCCTTTCAGCTAGCCAGCCTTAGCGCTGAACCTGGCTCACCGAGCGTGGTGCCACCGGCTGGTTGTCGTTGGAGATg                                                   >  2:130808/1‑138 (MQ=255)
                                                                                               tcACAGGCGGCACAACATTATCGGCGAACAAAGAAAAGCCCCGCG‑TCGATCGCGGGGCTTGTCCTTTCAGCTAGCCAGCCTTAGCGCTGAACCTGGCTCACCGAGCGTGGTGCCACCGGCTGGTTGTCGTTGGAGATg                                                   <  1:130808/138‑1 (MQ=255)
                                                                                                        gcACAACATTATCGGCGAACAAAGAAAAGCCCCGCG‑TCGATCGCGGGGCTTGTCCTTTCAGCTAGCCAGCCTTAGCGCTGAACCTGGCTCACCGAGCGTGGTGCCACCGGCTGGTTGTCGTTg                                                         >  7:255934/1‑123 (MQ=255)
                                                                                                        gcACAACATTATCGGCGAACAAAGAAAAGCCCCGCG‑TCGATCGCGGGGCTTGTCCTTTCAGCTAGCCAGCCTTAGCGCTGAACCTGGCTCACCGAGCGTGGTGCCACCGGCTGGTTGTCGTTg                                                         <  8:255934/123‑1 (MQ=255)
                                                                                                          acaacaTTATCGGCGAACAAAGAAAAGCCCCGCG‑TCGATCGCGGGGCTTGTCCTTTCAGCTAGCCAGCCTTAGCGCTGAACCTGGCTCACCGAGCGTGGTGCCACCGGCTGGTTGTCGTTGGAGATGGTCACCTCCACCCGACGGTTCt                             <  6:98930/149‑1 (MQ=255)
                                                                                                            aacaTTATCGGCGAACAAAGAAAAGCCCCGCG‑TCGATCGCGGGGCTTGTCCTTTCAGCTAGCCAGCCTTAGCGCTGAACCTGGCTCACCGAGCGTGGTGCCACCGGCTGGTTGTCGTTGGAGATGGTCACCTCCACCCGACGGTTCTgc                           <  1:222618/149‑1 (MQ=255)
                                                                                                               aTTATCGGCGAACAAAGAAAAGCCCCGCG‑TCGATCGCGGGGCTTGTCCTTTCAGCTAGCCAGCCTTAGCGCTGAACCTGGCTCACCGAGCGTGGTGCCACCGGCTGGTTGTCGTTGGAGATGGTCACCTCCACCCGACGGTTCTGCGCa                        >  5:72752/1‑149 (MQ=255)
                                                                                                                    ctgCGAACAATGAAAAGCCCCGCT‑TCGATCGCGGGGCTTGTCCTTTCAGCTAGCCAGCCTTAGCTCTTAACCTGGCTCACCGAGCGTGGTGCCACCGGCTGGTTGTCGTTGGAGATGTTCACCTCCACCCGACGGTTCTGCGCACGTc                    <  8:12152/146‑1 (MQ=255)
                                                                                                                               gAAAAGCCCCGCG‑TCGATCGCGGGGCTTGTCCTTTCAGCTAGCCAGCCTTAGCGCTGAACCTGGCTCACCGAGCGTGGTGCCACCGGCTGGTTGTCGTTGGAGATGGTCACCTCCACCCGACGGTTCTGCGCACGTCCCGAGTTGCTc         >  3:239610/1‑148 (MQ=255)
                                                                                                                                aaaaGCCCCGCG‑TCGATCGCGGGGCTTGTCCTTTCAGCTAGCCAGCCTTAGCGCTGAACCTGGCTCACCGAGCGTGGTGCCACCGGCTGGTTGTCGTTGGAGATGGTCACCTCCACCCGACGGTTCTGCGCACGTCCCGAGTTGCTc         >  4:254097/1‑147 (MQ=255)
                                                                                                                                 aaaGCCCCGCG‑TCGATCGCGGGGCTTGTCCTTTCAGCTAGCCAGCCTTAGCGCTGAACCTGGCTCACCGAGCGTGGTGCCACCGGCTGGTTGTCGTTGGAGATGGTCACCTCCACCCGACGGTTCTGCGCACGTCCCGAGTTGCTCGAg      <  6:72752/149‑1 (MQ=255)
                                                                                                                                   aGCCCCGCG‑TCGATCGCGGGGCTTGTCCTTTCAGCTAGCCAGCCTTAGCGCTGAACCTGGCTCACCGAGCGTGGTGCCACCGGCTGGTTGTCGTTGGAGATGGTCACCTCCACCCGACGGTTCTGCGCACGTCCCGAGTTGCTCGAGtt    <  6:219362/149‑1 (MQ=255)
                                                                                                                                    gCCCCGCG‑TCGATCGCGGGGCTTGTCCTTTCAGCTAGCCAGCCTTAGCGCTGAACCTGGCTCACCGAGCGTGGTGCCACCGGCTGGTTGTCGTTGGAGATGGTCACCTCCACCCGACGGTTCTGCGCACGTCCCGAGTTGCTCGa       >  2:167236/1‑145 (MQ=255)
                                                                                                                                    gCCCCGCG‑TCGATCGCGGGGCTTGTCCTTTCAGCTAGCCAGCCTTAGCGCTGAACCTGGCTCACCGAGCGTGGTGCCACCGGCTGGTTGTCGTTGGAGATGGTCACCTCCACCCGACGGTTCTGCGCACGTCCCGAGTTGCTCGa       <  1:167236/145‑1 (MQ=255)
                                                                                                                                     ccccGCGATCGA‑CGCGGGGCTTGTCCTTTCAGCTAGCCAGCCTTAGCGCTGAACCTGGCTCACCGAGCGTGGTGCCACCGGCTGGTTGTCGTTGGAGATGGTCACCTCCACCCGACGGTTCTGCGCACGTCCCGAGTTGCTCGAGTTGt  >  1:65155/1‑149 (MQ=255)
                                                                                                                                            |                                                                                                                                              
CATGGGCGAGGAAACACCGCGGCAGGTGCAGTGAGGTTTCGGCGCAGCATTTTTGGCGCCCGCGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGGCACAACATTATCGGCGAACAAAGAAAAGCCCCGCG‑TCGATCGCGGGGCTTGTCCTTTCAGCTAGCCAGCCTTAGCGCTGAACCTGGCTCACCGAGCGTGGTGCCACCGGCTGGTTGTCGTTGGAGATGGTCACCTCCACCCGACGGTTCTGCGCACGTCCCGAGTTGCTCGAGTTGT  >  NC_002947/1707149‑1707430

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑