| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 278,742:1 | +A | 100% | intergenic (‑237/+77) | PP_0224 ← / ← sctC | DszC family monooxygenase/sulfur compound ABC transporter ATP‑binding protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 278,742 | 1 | . | A | 94.9% | 118.6 / ‑0.3 | 39 | intergenic (‑237/+77) | PP_0224/sctC | DszC family monooxygenase/sulfur compound ABC transporter ATP‑binding protein |
| Reads supporting (aligned to +/- strand): ref base . (0/2); new base A (19/18); total (19/20) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.87e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
CAGCTGATCGCGACAGACGTGTGCTGGTCCGGTGGTCCGGTATATATACCCTAATGATTTATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGT‑TACAGAGGTGCCGGGCTCTTCGCGGGT‑AA‑CCCGCTCCTACACACATGCCTCAAGATCTGTGCACTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGAACAGGTTTACTCGGAGGCCGCAGTCCCGAGGAACTTGCGCAAGAACTGCCGCGTACGCTCTTCCTTCGGCGCT > NC_002947/278604‑278885 | cAGCTGATCGCGACAGACGTGTGCTGGTCCGGTAGTCCGGTATATATACCCTAATGATTTATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGT‑TACAGAGGTGCCGGGCTCTTCGCGGGTAAA‑CCCGc < 1:228459/147‑1 (MQ=255) aGCTGATCGCGACAGACGTGTGCTGGTCCGGTGGTCCGGTATATATACCCTAATGATTTATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTATACAGAGGTGCCGGGCTCTTCGCGGGTAAA‑CCCGCTc < 1:65473/149‑1 (MQ=255) gCTGATCGCGACAGACGTGTGCTGGTCCGGTGGTCCGGTATATATACCCTAATGATTTATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGT‑TACAGAGGTGCCGGGCTCTTCGCGGGTAAA‑CCCGCTcc > 7:104304/1‑148 (MQ=255) gcgACAGACGTGTGCTGGTCCGGTGGTCCGGTATATATACCCTAATGATTTATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGT‑TACAGAGGTGCCGGGCTCTTCGCGGGTAAA‑CCCGCTCCTacacac < 2:291430/148‑1 (MQ=255) ccaCAGACGTGTGCTGGTCCGGTGGTCCGGTATATATACCCTAATGATTTATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGT‑TACAGAGGTGCCGGGCTCTTCGCGGGTAAA‑CCCGCTCCTACACACAt < 5:325660/147‑1 (MQ=255) aGACGTGTGCTGGTCCGGTGGTCCGGTATATATACCCTAATGATTTATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGT‑TACAGAGGTGCCGGGCTCTTCGCGGGTAAA‑CCCGCTCCTACACACATGCCt > 2:112459/1‑149 (MQ=255) tggtccggtggtccggTATATATACCCTAATGATTTATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGT‑TACAGAGGTGCCGGGCTCTTCGCGGGTAAA‑CCCGCTCCTACACACATTCCTCAAGaaaagt > 5:243008/1‑144 (MQ=255) gtccggtggtccggtATATATACCCTAATGATTTATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGT‑TACAGAGGTGCCGGGCTCTTCGCGGGTAAA‑CCCGCTCCTACACACATGCCTCAAGATCtgt < 3:162885/147‑1 (MQ=255) tccggtATATAAACAATAATGATTTATAAAATATTTAAGAACTAACTTTTTCGAAAATTTATAGAAGGGGGATTTGT‑TACAGAGCTGCCGGGCACTTCGCGGGTAAA‑CCCGCACCTCCGAACATGCCTCAAGATGTGTACACTGttgct > 2:210220/1‑145 (MQ=255) tatatataCCCTAATGATTTATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGT‑TACAGAGGTGCCGGGCTCTTCGCGGGTAAA‑CCAGCTCCTAAACAAATTACTCAAGATATGTTCACTGTAGGAGCggg > 5:308554/1‑149 (MQ=255) ccTAATGATTTATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGT‑TACAGAGGTGCCGGGCTCTTCGCGGGTAAA‑CCCGCTCCTACACACATGCCTCAAGATCt > 6:221255/1‑122 (MQ=255) ccTAATGATTTATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGT‑TACAGAGGTGCCGGGCTCTTCGCGGGTAAA‑CCCGCTCCTACACACATGCCTCAAGATCt < 5:221255/122‑1 (MQ=255) ccTAATGATTTATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGT‑TACAGAGGTGCCGGGCTCTTCGCGGGTAAA‑CCCGCTCCTACACACATGCCTCAAGATCTGTGCACTGTAGGAGCGGGTTTACCcgc > 6:131170/1‑149 (MQ=255) ccTAATGATTTATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGT‑TACAGAGGTGCCGGGCTCTTCGCGGGTAAA‑CCCGCTCCTACACACATGCCTCAAGATCTGTGCACTGTAGGAGCGGGTTTACCcgc > 1:191778/1‑149 (MQ=255) ccTAATGATTTATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGT‑TACAGAGGTGCCGGGCTCTTCGCGGGTAAA‑CACGCTCCTACACACATGCCTCAAGATCTGTGGACTGTAGGAGCGGGTTTACCcgc > 7:163443/1‑149 (MQ=255) tAATGATTTATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGT‑TACAGAGGTGCCGGGCTCTTCGCGGGTAAA‑CCCGCTCCTACACACAt > 8:101567/1‑108 (MQ=255) tAATGATTTATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGT‑TACAGAGGTGCCGGGCTCTTCGCGGGTAAA‑CCCGCTCCTACACACAt < 7:101567/108‑1 (MQ=255) aaTGATTTATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGT‑TACAGAGGTGCCGGGCTCTTCGCGGGTAAA‑CCCGCTCCTACACACATGCCTCAAGATCTGTGCACTGTAGGAGCGGGTTTACCCGCGa > 1:66803/1‑148 (MQ=255) aTTTATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGT‑TACAGAGGTGCCGGGCTCTTCGCGGGTAAA‑CACGATCCTAAACACATGACTCAAGATCTGTGCACTGTAGGAGCGGGTTTACCAGCGAAAgg > 7:317324/1‑148 (MQ=255) ttATAAAATTTTTATGAACTAACTTTTTGTAATATTTATAGAAGGGGGATTTGT‑TACAGAGGTGCCGGGCTCTTCGCGGGTAAA‑CCCGCTCCTACACACATGCCTCAAGATCTGTGCACTGTAGGAGCGGGTTTACCCGCGAAAGGGc < 8:104304/148‑1 (MQ=255) tttATTAACTAACTTTTTTTAATATTTATAGAAGGGGGATTTTT‑TACAGAGGTGCCGGGCTCTTCGCGGGTAAA‑CCCGCTCCTACACACTTGCCTCAAGTTCTGTGCACTGTAGTAGCGGTTTTACCCGCGAAAGGGCCGGAACAGGt < 6:308554/148‑1 (MQ=255) tttATTAACTAACTTTTTGTAATTTTAATACACGGGGGTTTTGT‑AACAGAGGGCCCGGGCTCTTCGCGGTT‑AACCCCGCTCCAACACACATCCCCCACTATGTGTGCACTTTCGGCGGGGTTTTACCCGCGAAAGGGCCGGAACAGGt < 2:191778/148‑1 (MQ=255) tttATGAACTAACTTTTTGTAATATTTTTAGAAGGGGTATTTGT‑TACAGTGGGGCCGGGCTCTTCGCGGGT‑AACCCCGCTCCAACACACATGCCTCAATTTCTGTGCACTTTCGGTGGGGTTTTACCCGCGAAAGGGCCGGAACAGGt < 8:163443/148‑1 (MQ=255) ttATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGT‑TACAGAGGTGCCGGGCTCTTCGCGGGTAAA‑CCCGCTCCTACACACATGCCTCAAGATCTGTGCACTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGAACAGGtt < 7:7435/148‑1 (MQ=255) actaactTTTTGGAATATTTATAGAAGGGGGATTTGT‑TACAGAGGTGCCGGGCTCTTCGCGGGTAAA‑CCCGCTCCTACACACATGCCTCAAGATCTGTGCACTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGAACAGGTTTACTCg > 4:229428/1‑148 (MQ=255) aataagtTTTTGTAATTTTAATATATGGGGCTTTTGT‑TAAATTGGTGCGGGGTTTTTCGCGGGTAAA‑CCCGCTCATACACAAATGCCTCATTTTTTTTTAACTGTTGGAGCGGTTTTACCCGCGAAAGGGCCGGAACAGGTTTACTCg < 2:109768/146‑1 (MQ=255) taactTTTTGGAATATTTATAGAAGGGGGATTTGT‑TACAGAGGTGCCGGGCTCTTCGCGGGTAAA‑CCCGCTCCTACACACATGCCTCAAGATCTGTGCa < 8:336553/99‑1 (MQ=255) taactTTTTGGAATATTTATAGAAGGGGGATTTGT‑TACAGAGGTGCCGGGCTCTTCGCGGGTAAA‑CCCGCTCCTACACACATGCCTAAAGATCTGTGCa > 7:336553/1‑99 (MQ=255) ctTTTTGGAATATTTATAGAAGGGGGATTTGT‑TACAGAGGTGCCGGGCTCTTCGCGGGTAAA‑CCCGCTCCTACACACATCCCTCAAGATCTGTGCACTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGAACAGGTTTACTCGGAGGcc < 1:112459/149‑1 (MQ=255) ttttGGAATATTTATAGAAGGGGGATTTGT‑TACAGAGGTGCCGGGCTCTTCGCGGGTAAA‑ACCGCTCCTACACACATGACGCAAGATCTGTGCACTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGAACAGGTTTACTCGGAGGCCg > 5:211426/1‑148 (MQ=39) ggAATATTTATAGAAGGGGGATTTGT‑TACAGAGGTGCCGGGCTCTTCGCGGGTAAA‑CCCGCTCCTACACACATGCCTCAAGATCTGTGCACTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGAACAGGTTTACTCGGAGGCCGCAGTc > 2:41572/1‑149 (MQ=255) gAATATTTATAGAAGGGGGATTTGT‑TACAGAGGTGCCGGGCTCTTCGCGGGTAAA‑CCCGCTCCTACACACATGCCTCAAGATCTGTGCACTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGAACAGGTTTACTCGGAGGCCGCAGTcc > 1:69170/1‑149 (MQ=255) aaGGGGGATTTGT‑TACAGAGGTGCCGGGCTCTTCGCGGGTAAA‑CCCGCTCCTACACACATGCCTCAAGATCTGTGCACTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGAACAGGTTTACTCGGAGGCCGCAGTCCCGAGGAACTTgc > 3:109590/1‑149 (MQ=255) aaGGGGGATTTGT‑TACAGAGGTGCCGGGCTCTTCGCGGGTAAA‑CCCGCTCCTACACACATGCCTCAAGATCTGTGCACTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGAACAGGTTTACTCGGAGGCCGCAGTCCCGAGGAACTTgc < 1:41572/149‑1 (MQ=255) aGGGGGATTTGT‑TACAGAGGTGCCGGGCTCTTCGCGGGTAAA‑CCCGCTCCTACACACATGCCTCAAGATTTGTGCACTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGAACAGGTTTACTCGGAGGCCGCAGTCCCGAGGAACTTgcg < 2:69170/149‑1 (MQ=255) t‑tACAGAGGTGCCGGGCTCTTCGCGGGTAAA‑CCCGCTCCTACACACATGCCTCAAGATCTGTGCACTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGAACAGGTTTACTCGGAGGCCGCAGTCCCGAGGAACTTGCGCAAGAACTGc < 5:131170/148‑1 (MQ=255) ccGGGCTCTTCGCGGGTAAA‑CCCGCTCCTACACACATGCCTCAAGATCTGTGCACTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGAACAGGTTTACTCGGAGGCCGCAGTCCCGAGGAACTTGCGCAAGAACTGCCGCGTACGctct < 4:109590/149‑1 (MQ=255) ggCTGTTCGCGTGTAAA‑CCCGGTCGTACACACATGCCTCAAGCTCTTTACCGTGTTGTAGCGGGTTTACCCGCGAAAGGGCCGGATCAGGTTTACTCGGCGGCCGGAGTCCCGAGGAACTTGCGCAAGAACTGCCGCGTACGCTcttcc < 1:210220/149‑1 (MQ=255) cgGGTAAA‑CCCGCTCCTACACACATGCCTCAAGATCTGTGCACTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGAACAGGTTTACTCGGAGGCCGCAGTCCCGAGGAACTTGCGCAAGAACTGCCGCGTACGCTCTTCCTTCGGCGCt > 3:158925/1‑149 (MQ=255) | CAGCTGATCGCGACAGACGTGTGCTGGTCCGGTGGTCCGGTATATATACCCTAATGATTTATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGT‑TACAGAGGTGCCGGGCTCTTCGCGGGT‑AA‑CCCGCTCCTACACACATGCCTCAAGATCTGTGCACTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGAACAGGTTTACTCGGAGGCCGCAGTCCCGAGGAACTTGCGCAAGAACTGCCGCGTACGCTCTTCCTTCGGCGCT > NC_002947/278604‑278885 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |