Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,460,230:1 +A 6.6% coding (132/1446 nt) PP_3075 ← transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,460,2301.A 6.6% 244.0 / 10.4 61coding (132/1446 nt)PP_3075transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base . (28/29);  new base A (2/2);  total (30/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.18e-01

GGCTGCCGGGGATCACTGTTTCGCACGGCTGGCCGATGGCACTGGCAGCGTCGGCCAGGCCAAAGCGTCGGGCGTAGCGTTCGTTCATCCAGACGATGCGTGCCTGGCGGTCGA‑CGATCACCGTGCCCTCGCTGGACTGTTCGATGATC‑TCGAACAGCGAGCGGATCGCCAGTTGCCGTACCTGGGGGTAGTCCTTGAGGCTGTCGCTGTCGTGCATGGGTGAACATCCTGAATCATGTATTGGCACAAGCCCGCGAAAAGGCCAGGCCTGAAAACTCATCGTCGGGGGTCAAACG  >  NC_002947/3460082‑3460377
                                                                                                                                                      |                                                                                                                                                   
ggCTGCCGGGGATCACTGTTTCGCACGGCTGGCCGATGGCACTGGCAGCGTCGGCCAGGCCAAAGCGTCGGGCGTAGCGTTCGTTCATCCAGACGATGCGTGCCTGGCGGTCGA‑CGATCACCGTGCCCTCGCTGGACTGTTCGATGAtc                                                                                                                                                      >  5:867/1‑149 (MQ=255)
  cTGCCGGGGATCACTGTTTCGCACGGCTGGCCGATGGCACTGGCAGCGTCGGCCAGGCCAAAGCGTCGGGCGTAGCGTTCGTTCATCCAGACGATGCGTGCCTGGCGGTCGA‑CGATCACCGTGCCCTCGCTGGACTGTTCGATGAtc‑tc                                                                                                                                                   <  6:158307/149‑1 (MQ=255)
          gATCACTGTTTCGCACGGCTGGCCGATGGCACTGGCAGCGTCGGCCAGGCCAAAGCGTCGGGCGTAGCGTTCGTTCATCCAGACGATGCGTGCCTGGCGGTCGA‑CGATCACCGTGCCCTCGCTGGACTGTTCGATGATC‑TCGAACagcg                                                                                                                                           >  8:226388/1‑149 (MQ=255)
           aTCACTGTTTCGCACGGCTGGCCGATGGCACTGGCAGCGTCGGCCAGGCCAAAGCGTCGGGCGTAGCGTTCGTTCATCCAGACGATGCGTGCCTGGCGGTCGA‑CGATCACCGTGCCCTCGCTGGACTGTTCGATGATC‑TCGAACagcga                                                                                                                                          <  2:372025/149‑1 (MQ=255)
            tCACTGTTTCGCACGGCTGGCCGATGGCACTGGCAGCGTCGGCCAGGCCAAAGCGTCGGGCGTAGCGTTCGTTCATCCAGACGATGCGTGCCTGGCGGTCGA‑CGATCACCGTGCCCTCGCTGGACTGTTCGATGATC‑TCGAACagcgag                                                                                                                                         >  6:188965/1‑149 (MQ=255)
              aCTGTTTCGCACGGCTGGCCGATGGCACTGGCAGCGTCGGCCAGGCCAAAGCGTCGGGCGTAGCGTTCGTTCATCCAGACGATGCGTGCCTGGCGGTCGA‑CGATCACCGTGCCCTCGCTGGACTGTTCGATGATC‑TCGAACagcgagcg                                                                                                                                       >  5:343745/1‑149 (MQ=255)
              aCTGTTTCGCACGGCTGGCCGATGGCACTGGCAGCGTCGGCCAGGCCAAAGCGTCGGGCGTAGCGTTCGTTCATCCAGACGATGCGTGCCTGGCGGTCGA‑CGATCACCGTGCCCTCGCTGGACTGTTCGATGATC‑TCGAACagcgagc                                                                                                                                        <  7:226388/148‑1 (MQ=255)
               cTGTTTCGCACGGCTGGCCGATGGCACTGGCAGCGTCGGCCAGGCCAAAGCGTCGGGCGTAGCGTTCGTTCATCCAGACGATGCGTGCCTGGCGGTCGA‑CGATCACCGTGCCCTCGCTGGACTGTTCGA‑GATCATCGAACagtccag                                                                                                                                        >  8:214952/1‑142 (MQ=255)
               cTGTTTCGCACGGCTGGCCGATGGCACTGGCAGCGTCGGCCAGGCCAAAGCGTCGGGCGTAGCGTTCGTTCATCCAGACGATGCGTGCCTGGCGGTCGA‑CGATCACCGTGCCCTCGCTGGACTGTTCGA‑GATCATCGAACagtccag                                                                                                                                        <  7:214952/147‑6 (MQ=255)
                  tttCGCACGGCTGGCCGATGGCACTGGCAGCGTCGGCCAGGCCAAAGCGTCGGGCGTAGCGTTCGTTCATCCAGACGATGCGTGCCTGGCGGTCGATTGATCACCGTGCCCTCGCTGGACTGTTCGATGATC‑TCGAACAGCGAGCGGAt                                                                                                                                    <  1:278382/149‑1 (MQ=255)
                  tttCGCACGGCTGGCCGATGGCACTGGCAGCGTCGGCCAGGCCAAAGCGTCGGGCGTAGCGTTCGTTCATCCAGACGATGCGTGCCTGGCGGTCGA‑CGATCACCGTGCCCTCGCTGGACTGTTCGATGATC‑TCGAACAGCGAGCGGAt                                                                                                                                    <  6:101805/148‑1 (MQ=255)
                         cGGCTGGCCGATGGCACTGGCAGCGTCGGCCAGGCCAAAGCGTCGGGCGTAGCGTTCGTTCATCCAGACGATGCGTGCCTGGCGGTCGA‑CGATCACCGTGCCCTCGCTGGACTGTTCGATGATC‑TCGAACAGCGAGCGGATCGCCAGtt                                                                                                                            <  6:199653/149‑1 (MQ=255)
                                    tGGCACTGGCAGCGTCGGCCAGGCCAAAGCGTCGGGCGTAGCGTTCGTTCATCCAGACGATGCGTGCCTGGCGGTCGA‑CGATCACCGTGCCCTCGCTGGACTGTTCGATGATC‑TCGAACAGCGAGCGGATCGCCAGTTGCCGTACCTgg                                                                                                                 >  1:134726/1‑149 (MQ=255)
                                      gCACTGGCAGCGTCGGCCAGGCCAAAGCGTCGGGCGTAGCGTTCGTTCATCCAGACGATGCGTGCCTGGCGGTCGA‑CGATCACCGTGCCCTCGCTGGACTGTTCGATGATC‑TCGAACAGCGAGCGGATCGCCAGTTGCCGTACCTgggg                                                                                                               <  5:188965/149‑1 (MQ=255)
                                            gCAGCGTCGGCCAGGCCAAAGCGTCGGGCGTAGCGTTCGTTCATCCAGACGATGCGTGCCTGGCGGTCGA‑CGATCACCGTGCCCTCGCTGGACTGTTCGATGATC‑TCGAACAGCGAGCGGATCGCCAGTTGCCGTACCTGGGGGTAGTc                                                                                                         >  7:117344/1‑149 (MQ=255)
                                            gCAGCGTCGGCCAGGCCAAAGCGTCGGGCGTAGCGTTCGTTCATCCAGACGATGCGTGCCTGGCGGTCGA‑CGATCACCGTGCCCTCGCTGGACTGTTCGATGATC‑TCGAACAGCGAGCGGATCGCCAGTTGCCGTACCTGGGGGTAGTc                                                                                                         <  5:238597/149‑1 (MQ=255)
                                            gCAGCGTCGGCCAGGCCAAAGCGTCGGGCGTAGCGTTCGTTCATCCAGACGATGCGTGCCTGGCGGTCGA‑CGATCACCGTGCCCTCGCTGGACTGTTCGA‑GATCATCGAACagtc                                                                                                                                           >  1:16475/1‑113 (MQ=255)
                                            gCAGCGTCGGCCAGGCCAAAGCGTCGGGCGTAGCGTTCGTTCATCCAGACGATGCGTGCCTGGCGGTCGA‑CGATCACCGTGCCCTCGCTGGACTGTTCGA‑GATCATCGAACagtc                                                                                                                                           <  2:16475/115‑3 (MQ=255)
                                               gCGTCGGCCAGGCCAAAGCGTCGGGCGTAGCGTTCGTTCATCCAGACGATGCGTGCCTGGCGGTCGA‑CGATCACCGTGCCCTCGCTGGACTGTTCGATGATC‑TCGAACAGCGAGCGGATCGCCAGTTGCCGTACCTGGGGGTAGTCCtt                                                                                                      <  6:867/149‑1 (MQ=255)
                                                 gTCGGCCAGGCCAAAGCGTCGGGCGTAGCGTTCGTTCATCCAGACGATGCGTGCCTGGCGGTCGA‑CGATCACCGTGCCCTCGCTGGACTGTTCGATGATC‑TCGAACagcgagc                                                                                                                                        >  2:236405/1‑113 (MQ=255)
                                                 gTCGGCCAGGCCAAAGCGTCGGGCGTAGCGTTCGTTCATCCAGACGATGCGTGCCTGGCGGTCGA‑CGATCACCGTGCCCTCGCTGGACTGTTCGATGATC‑TCGAACagcgagc                                                                                                                                        <  1:236405/113‑1 (MQ=255)
                                                 gTCGGCCAGGCCAAAGCGTCGGGCGTAGCGTTCGTTCATCCAGACGATGCGTGCCTGGCGGTCGA‑CGATCACCGTGCCCTCGCTGGACTGTTCGATGATC‑TCGAACAGCGAGCGGATCGCCAGTTGCCGTACCTGGGGGTAGTCCTTGa                                                                                                    <  3:198427/149‑1 (MQ=255)
                                                   cGGCCAGGCCAAAGCGTCGGGCGTAGCGTTCGTTCATCCAGACGATGCGTGCCTGGCGGTCGA‑CGATCACCGTGCCCTCGCTGGACTGTTCGATGATC‑TCGAACAGCGAGCGGATCGCCAGTTGCCGTACCTGGGGGTAGTCCTTGAgg                                                                                                  <  2:134726/149‑1 (MQ=255)
                                                     gccaggccaAAGCGTCGGGCGTAGCGTTCGTTCATCCAGACGATGCGTGCCTGGCGGTCGA‑CGATCACCGTGCCCTCGCTGGACTGTTCGATGATC‑TCGAACAGCGAGCGGATCGCCAGTTGCCGTACCTGGGGGTAGTCCTTGAGgct                                                                                                >  5:308802/1‑149 (MQ=255)
                                                         ggccaAAGCGTCGGGCGTAGCGTTCGTTCATCCAGACGATGCGTGCCTGGCGGTCGA‑CGATCACCGTGCCCTCGCTGGACTGTTCGATGATC‑TCGAACAGCGAGCGGATCGCCAGTTGCCGTACCTGGGGGTAGTCCTTTAGgctgtcg                                                                                            <  6:343745/149‑1 (MQ=255)
                                                            caAAGCGTCGGGCGTAGCGTTCGTTCATCCAGACGATGCGTGCCTGGCGGTCGA‑CGATCACCGTGCCCTCGCTGGACTGTTCGATGATC‑TCGAACAGCGAGCGGATCGCCAGtt                                                                                                                            >  6:330147/1‑114 (MQ=255)
                                                            caAAGCGTCGGGCGTAGCGTTCGTTCATCCAGACGATGCGTGCCTGGCGGTCGA‑CGATCACCGTGCCCTCGCTGGACTGTTCGATGATC‑TCGAACAGCGAGCGGATCGCCAGTTGCCGTACCTGGGGGTAGTCCtt                                                                                                      <  8:127932/136‑1 (MQ=255)
                                                            caAAGCGTCGGGCGTAGCGTTCGTTCATCCAGACGATGCGTGCCTGGCGGTCGA‑CGATCACCGTGCCCTCGCTGGACTGTTCGATGATC‑TCGAACAGCGAGCGGATCGCCAGTTGCCGTACCTGGGGGTAGTCCtt                                                                                                      >  7:127932/1‑136 (MQ=255)
                                                            caAAGCGTCGGGCGTAGCGTTCGTTCATCCAGACGATGCGTGCCTGGCGGTCGA‑CGATCACCGTGCCCTCGCTGGACTGTTCGATGATC‑TCGAACAGCGAGCGGATCGCCAGTTGCCGTACCTGGGGGTAGTCCTTGAGgctgtcgctg                                                                                         >  7:59028/1‑149 (MQ=255)
                                                             aaaGCGTCGGGCGTAGCGTTCGTTCATCCAGACGATGCGTGCCTGGCGGTCGA‑CGATCACCGTGCCCTCGCTGGACTGTTCGATGATC‑TCGAACAGCGAGCGGATCGCCAGTTGCCGTACCTGGGGGTAGTCCTTGAGgctgtcgctgt                                                                                        >  6:234646/1‑149 (MQ=255)
                                                              aatcgtcgtGCGTAGCGTTAGTTCATCCAGACTATGCTTGCCTGGCGGTCGA‑CGATCACCTTGCCCTCTCTGGACTGTTCTATGATC‑TCGAACAGCGAGCGGATCGCCAGTTGCCGTACCTGGGGTTAGTCCTTGAGgctgtcgctgtc                                                                                       <  2:317247/146‑1 (MQ=255)
                                                              aaGCGTCGGGCGTAGCGTTCGTTCATCCAGACGATGCGTGCCTGGCGGTCGA‑CGATCACCGTGCCCTCGCTGGACTGTTCGATGATC‑TCGAACAGCGAGCGGATCGCCAGTTGCCGTACCTGGGGGTAGTCCTTGAGgctgtcgctgtc                                                                                       <  5:371245/149‑1 (MQ=255)
                                                                      ggCGTAGCGTTCGTTCATCCAGACGATGCGTGCCTGGCGGTCGA‑CGATCACCGTGCCCTCGCTGGACTGTTCGATGATC‑TCGAACAGCGAGCGGATCGCCAGTTGCCGTACCTGGGGGTAGTCCTTGAGGCTGTCGCTGTCGTGCATgg                                                                               <  8:59028/149‑1 (MQ=255)
                                                                        cGTAGCGTTCGTTCATCCAGACGATGCGTGCCTGGCGGTCGA‑CGATCACCGTGCCCTCGCTGGACTGTTCGATGATC‑TCGAACAGCGAGCGGATCGCCAGTTGCCGTACCTGGGGGTAGTCCTTGAGGCTGTCGCTGTCGTGCATGGGt                                                                             <  6:308802/149‑1 (MQ=255)
                                                                               ttcgttcATCCAGACGATGCGTGCCTGGCGGTCGA‑CGATCACCGTGCCCTCGCTGGACTGTTCGATGATC‑TCGAACAGCGAGCGGATCGCCAGTTGCCGTACCTGGGGGTAGTCCTTGAGGCTGTCGCTGTCGTGCATGGGTGAACATc                                                                      <  8:117344/149‑1 (MQ=255)
                                                                                tcgttcATCCAGACGATGCGTGCCTGGCGGTCGA‑CGATCACCGTGCCCTCGCTGGACTGTTCGATGATC‑TCGAACAGCGAGCGGATCGCCAGTTGCCGTACCTGGGGGTAGTCCTTGAGGCTGTCGCTGTCGTGCATGGGt                                                                             >  6:87156/1‑141 (MQ=255)
                                                                                tcgttcATCCAGACGATGCGTGCCTGGCGGTCGA‑CGATCACCGTGCCCTCGCTGGACTGTTCGATGATC‑TCGAACAGCGAGCGGATCGCCAGTTGCCGTACCTGGGGGTAGTCCTTGAGGCTGTCGCTGTCGTGCATGGGt                                                                             <  5:87156/141‑1 (MQ=255)
                                                                                 cgttcATCCAGACGATGCGTGCCTGGCGGTCGA‑CGATCACCGTGCCCTCGCTGGACTGTTCGATGATC‑TCGAACAGCGAGCGGATCGCCAGTTGCCGTACCTGGGGGTAGTCCTTGAGGCTGTCGCTGTCGTGCATGGGTGAACATcc                                                                     <  7:252433/148‑1 (MQ=255)
                                                                                         cAGACGATGCGTGCCTGGCGGTCGA‑CGATCACCGTGCCCTCGCTGGACTGTTCGATGATC‑TCGAACAGCGAGCGGATCGCCAGTTGCCGTACCTGGGGGTAGTCCTTGAGGCTGTCGCTGTCGTGCATGGGTGAACATCCTGAATCAt                                                             <  5:234646/148‑1 (MQ=255)
                                                                                          aGACGATGCGTGCCTGGCGGTCGA‑CGATCACCGTGCCCTCGCTGGACTGTTCGATGATC‑TCGAACAGCGAGCGGATCGCCAGTTGCCGTACCTGGGGGTAGTCCTTGAGGCTGTCGCTGTCGTGCATGGGTGAACATCCt                                                                    >  4:326948/1‑140 (MQ=255)
                                                                                          aGACGATGCGTGCCTGGCGGTCGA‑CGATCACCGTGCCCTCGCTGGACTGTTCGATGATC‑TCGAACAGCGAGCGGATCGCCAGTTGCCGTACCTGGGGGTAGTCCTTGAGGCTGTCGCTGTCGTGCATGGGTGAACATCCt                                                                    <  3:326948/140‑1 (MQ=255)
                                                                                            aCGATGCGTGCCTGGCGGTCGA‑CGATCACCGTGCCCTCGCTGGACTGTTCGATGATC‑TCGAACAGCGAGCGGATCGCCAGTTGCCGTACCTGGGGGTAGTCCTTGAGGCTGTCGCTGTCGTGCATGGGTGAACATCCTGAATCATGTAt                                                         <  3:101155/149‑1 (MQ=255)
                                                                                                   gTGCCTGGCGGTCGA‑CGATCACCGTGCCCTCGCTGGACTGTTCGATGATC‑TCGAACAGCGAGCGGATCGCCAGTTGCCGTACCTGGGGGTAGTCCTTGAGGCTGTCGCTGTCGTGCATGGGTGAACATCCTGAATCATGTATTg                                                       >  6:259900/1‑144 (MQ=255)
                                                                                                   gTGCCTGGCGGTCGA‑CGATCACCGTGCCCTCGCTGGACTGTTCGATGATC‑TCGAACAGCGAGCGGATCGCCAGTTGCCGTACCTGGGGGTAGTCCTTGAGGCTGTCGCTGTCGTGCATGGGTGAACATCCTGAATCATGTATTg                                                       <  5:259900/144‑1 (MQ=255)
                                                                                                         ggCGGTCGA‑CGATCACCGTGCCCTCGCTGGACTGTTCGATGATC‑TCGAACAGCGAGCGGATCGCCAGTTGCCGTACCTGGGGGTAGTCCTTGAGGCTGTCGCTGTCGTGCATGGGTGAACATCCTGaa                                                                 >  6:291780/1‑128 (MQ=255)
                                                                                                         ggCGGTCGA‑CGATCACCGTGCCCTCGCTGGACTGTTCGATGATC‑TCGAACAGCGAGCGGATCGCCAGTTGCCGTACCTGGGGGTAGTCCTTGAGGCTGTCGCTGTCGTGCATGGGTGAACATCCTGaa                                                                 <  5:291780/128‑1 (MQ=255)
                                                                                                            ggTCGA‑CGATCACCGTGCCCTCGCTGGACTGTTCGATGATC‑TCGAACAGCGAGCGGATCGCCAGTTGCCGTACCTGGGGGTAGTCCTTGAGGCTGTCGCTGTCGTGCATGGGTGAACATCCTGAATCATGTATTGGCACAAGCCcgcg                                          >  5:207085/1‑148 (MQ=255)
                                                                                                                 a‑cgaTCACCGTGCCCTCGCTGGACTGTTCGATGATC‑TCGAACAGCGAGCGGATCGCCACTTGCCGTACCTGGGGGTAGTCATTGAGGCTGTCGAAGGCGTGCATGGGTGCACATCCTGAATCATGTATTGCCACACGCAAGCGAAAagg                                    >  7:165734/1‑149 (MQ=255)
                                                                                                                      tCACCGTGCCCTCGCTGGACTGTTCGATGATC‑TCGAACAGCGAGCGGATCGCCAGTTGCCGTACCTGGGGGTAGTCCTTGAGGCTGTCGCTGTCGTGCATGGGTGAACATCCTGAATCATGTATTGGCACAAGCCCGCGAAAaggccag                                >  5:170404/1‑149 (MQ=255)
                                                                                                                          cGTGCCCTCGCTGGACTGTTCGATGATC‑TCGAACAGCGAGCGGATCGCCAGTTGCCGTACCTGGGGGTAGTCCTTGa                                                                                                    <  8:294497/77‑1 (MQ=255)
                                                                                                                          cGTGCCCTCGCTGGACTGTTCGATGATC‑TCGAACAGCGAGCGGATCGCCAGTTGCCGTACCTGGGGGTAGTCCTTGa                                                                                                    >  7:294497/1‑77 (MQ=255)
                                                                                                                          cGTGCCCTCGCTGGACTGTTCGATGATC‑TCGAACAGCGAGCGGATCGCCAGTTGCCGTACCTGGGGGTAGTCCTTGAGGCTGTCGCTGTCGTGCATGGGTGAACATCCTGAATCATGTATTGGCACAAGCCCGCGAAAAGGCCAGGCCt                            >  4:176124/1‑149 (MQ=255)
                                                                                                                              cccTCGCTGGACTGTTCGATGATC‑TCGAACAGCGAGCGGATCGCCAGTTGCCGTACCTGGGGGTAGTCCTTGAGGCTGTCGCTGTCGTGCATGGGTGAACATCCTGAATCATGTATTGGCACAAGCCCGCGAAAaggcc                                  >  5:322490/1‑139 (MQ=255)
                                                                                                                              cccTCGCTGGACTGTTCGATGATC‑TCGAACAGCGAGCGGATCGCCAGTTGCCGTACCTGGGGGTAGTCCTTGAGGCTGTCGCTGTCGTGCATGGGTGAACATCCTGAATCATGTATTGGCACAAGCCCGCGAAAaggcc                                  <  6:322490/139‑1 (MQ=255)
                                                                                                                              cccTCGCTGGACTGTTCGATGATC‑TCGAACAGCGAGCGGATCGCCAGTTGCCGTACCTGGGGGTAGTCCTTGAGGCTGTCGCTGTCGTGCATGGGTGAACATCCTGAATCATGTATTGGCACAAGCCCGCGAAAAGGCCAGGCCTGaa                         >  3:159983/1‑148 (MQ=255)
                                                                                                                               ccTAGCTGGACTGTTCGATTATC‑TCGAACATCGAGCGTATCGCCAGTTGCCGTACCTGGTGTTATTCCTTGAGGCTGTCGCTGTCGTGCATGGGTGAACATCCTCAATCATGTATTGGCACAAGCCCGCGAAAAGGCCAGGCCTGaaaa                       <  7:313420/149‑1 (MQ=255)
                                                                                                                                cTCGCTGGACTGTTCGATGATC‑TCGAACAGCGAGCGGATCGCCAGTTGCCGTACCTGGGGGTAGTCCTTGAGGCTGTCGCTGTCGTGCATGGGTGAACATCCTGAATCATGTATTGGCACAAGCCCGCGAAAAGGCCAGGCCTGAAAAc                      >  6:113411/1‑149 (MQ=255)
                                                                                                                                          tGTTCGATGATC‑TCGAACAGCGAGCGGATCGCCAGTTGCCGTACCTGGGGGTAGTCCTTGAGGCTGTCGCTGTCGTGCATGGGTGAACATCCTGAATCATGTATTGGCACAAGCCCGCGAAAAGGCCAGGCCTGaa                         <  3:266997/136‑1 (MQ=255)
                                                                                                                                          tGTTCGATGATC‑TCGAACAGCGAGCGGATCGCCAGTTGCCGTACCTGGGGGTAGTCCTTGAGGCTGTCGCTGTCGTGCATGGGTGAACATCCTGAATCATGTATTGGCACAAGCCCGCGAAAAGGCCAGGCCTGAAAACTCATCGTCgg            >  1:238865/1‑149 (MQ=255)
                                                                                                                                          tGTTCGATGATC‑TCGAACAGCGAGCGGATCGCCAGTTGCCGTACCTGGGGGTAGTCCTTGAGGCTGTCGCTGTCGTGCATGGGTGAACATCCTGAATCATGTATTGGCACAAGCACGCGAAAAGGCCAGGCCTGaa                         >  4:266997/1‑136 (MQ=255)
                                                                                                                                               gatgatC‑TCGAACAGCGAGCGGATCGCAAGTTGCCGTACCTGGGGGTAGTCCTTGAGGCTGTCGCTGTCGTGCATGGGTGAACATCCTGAATCATGTATTGGCACAAGCCCGCGAAAAGGCCAGGCCTGAAAACTCATCGTCGGGGGt        >  2:213353/1‑148 (MQ=255)
                                                                                                                                                  gatC‑TCGAACAGCGAGCGGATCGCCAGTTGCCGTACCTGGGGGTAGTCCTTGAGGCTGTCGCTGTCGTGCATGGGTGAACATCCTGAATCATGTATTGGCACAAGCCCGCGAAAAGGCCAGGCCTGAAAACTCATCGTCGGGGGTCaaa    >  5:201647/1‑149 (MQ=255)
                                                                                                                                                   atC‑TCGAACAGCGAGCGGATCGCCAGTTGCCGTACCTGGGGGTAGTCCTTGAGGCTGTCGCTGTCGTGCATGGGTGAACATCCTGAATCATGTATTGGCACAAGCCCGCGAAAAGGCCAGGCCTGAAAACTCATCGTCGGGGGTCAAAc   >  8:142962/1‑149 (MQ=255)
                                                                                                                                                    tc‑tcGAACAGCGAGCGGATCGCCAGTTGCCGTACCTGGGGGTAGTCCTTGAGGCTGTCGCTGTCGTGCATGGGTGAACATCCTGAATCATGTATTGGCACAAGCCCGCGAAAAGGCCAGGCCTGAAAACTCATCGTCGGGGGTCAAACg  >  5:208994/1‑149 (MQ=255)
                                                                                                                                                     c‑tcGAACAGCGAGCGGATCGCCAGTTGCCGTACCTGGGGGTAGTCCTTGAGGCTGTCGCTGTCGTGCATGGGTGAACATCCTGAATCATGTATTGGCACAAGCCCGCGAAAAGGCCAGGCCTGAAAAc                      <  5:242625/128‑1 (MQ=255)
                                                                                                                                                     c‑tcGAACAGCGAGCGGATCGCCAGTTGCCGTACCTGGGGGTAGTCCTTGAGGCTGTCGCTGTCGTGCATGGGTGAACATCCTGAATCATGTATTGGCACAAGCCCGCGAAAAGGCCAGGCCTGAAAAc                      >  6:242625/1‑128 (MQ=255)
                                                                                                                                                      |                                                                                                                                                   
GGCTGCCGGGGATCACTGTTTCGCACGGCTGGCCGATGGCACTGGCAGCGTCGGCCAGGCCAAAGCGTCGGGCGTAGCGTTCGTTCATCCAGACGATGCGTGCCTGGCGGTCGA‑CGATCACCGTGCCCTCGCTGGACTGTTCGATGATC‑TCGAACAGCGAGCGGATCGCCAGTTGCCGTACCTGGGGGTAGTCCTTGAGGCTGTCGCTGTCGTGCATGGGTGAACATCCTGAATCATGTATTGGCACAAGCCCGCGAAAAGGCCAGGCCTGAAAACTCATCGTCGGGGGTCAAACG  >  NC_002947/3460082‑3460377

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑