Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,735,143:1 +T 8.2% coding (40/3093 nt) PP_3302 → RND family transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,735,1431.T 8.2% 184.8 / 11.8 49coding (40/3093 nt)PP_3302RND family transporter
Reads supporting (aligned to +/- strand):  ref base . (26/19);  new base T (2/2);  total (28/21)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TGACCTGCACGCTGGCGATCGGATCGTGGCACTGGGTGCGCATCTGCTGCGCGATGGCGTCGAGGTCATGGAACAGGCTCAAACAGCTCAGGTCATGGGGCACC‑AACCATGAGCCAGCTGCGTTTCAACTTATCCGCGCTCGCAGTAC‑GCGAGCGATCAATCACCCTCTTCCTGATTGTGCTGATCGCCTTCGCCGGCACGCTGGCCTTTTTCAAGCTGGGCCGTGCAGAAGATCCGCCCTTCACCGTCAAGCAGATGACGATTATCACTGCCTGGCCCGGCGCCACTGCGCAG  >  NC_002947/3734996‑3735289
                                                                                                                                                     |                                                                                                                                                  
tGACCTGCACGCTGGCGATCGGATCGTGGCACTGGGTGCGCATCTGCTGCGCGATGGCGTCGAGGTCATGGAACAGGCTCAAACAGCTCAGGTCATGGGGCACC‑AACCATGAGCCAGCTGCGTTTCAACTTATCCGCGCTCGCAGTAc‑g                                                                                                                                                   >  6:367782/1‑149 (MQ=255)
   ccTGCACGCTGGCGATCGGATCGTGGCACTGGGTGCGCATCTGCTGCGCGATGGCGTCGAGGTCATGGAACAGGCTCAAACAGCTCAGGTCATGGGTCACC‑AACCATGAGCCAGCTGCGTTTCAACTTATCCGCGCTCGCAGTAC‑gcga                                                                                                                                                <  4:350594/149‑1 (MQ=255)
         cGCTGGCGATCGGATCGTGGCACTGGGTGCGCATCTGCTGCGCGATGGCGTCGAGGTCATGGAACAGGCTCAAACAGCTCAGGTCATGGGGCACCAAACCATGAGCCAGCTGCGTTTCAACTTATCCGCGCTCGCAGTAC‑GCGAGCGat                                                                                                                                           <  1:92220/149‑1 (MQ=255)
            tGGCGATCGGATCGTGGCACTGGGTGCGCATCTGCTGCGCGATGGCGTCGAGGTCATGGAACAGGCTCAAACAGCTCAGGTCATGGGGCACC‑AACCATGAGCCAGCTGCGTTTCAACTTATCCGCGCTCGCAGTAC‑GCGAGCGatcaat                                                                                                                                       >  5:139561/1‑149 (MQ=255)
                gatcggatcgTGGCACTGGGTGCGCATCTGCTGCGCGATGGCGTCGAGGTCATTGAACAGGCTCAAACAGCTCAGGTCATGGGGCACC‑AACCATGAGCCAGCTGCGTTTCAACTTATCCGCGCTCGC‑GTACTgcgagcgc                                                                                                                                            <  6:218579/140‑2 (MQ=255)
                gatcggatcgTGGCACTGGGTGCGCATCTGCTGCGCGATGGCGTCGAGGTCATGGAACAGGCTCAAACAGCTCAGGTCATGGGGCACC‑AACCATGAGCCAGCTGCGTTTCAACTTATCCGCGCTCGC‑GTACTgcgagcgc                                                                                                                                            >  5:218579/1‑139 (MQ=255)
                 atcggatcgTGGCACTGGGTGCGCATCTGCTGCGCGATGGCGTCGAGGTCATGGAACAGGCTCAAACAGCTCAGGTCATGGGGCACC‑AACCATGAGCCAGCTGCGTTTCAACTTATCCGCGCTCGCAGTAC‑GCGAGCGATCAATCAccc                                                                                                                                  >  3:299237/1‑149 (MQ=255)
                       tcgTGGCACTGGGTGCGCATCTGCTGCGCGATGGCGTCGAGGTCATGGAACAGGCTCAAACAGCTCAGGTCATGGGGCACC‑AACCATGAGCCAGCTGCGTTTCAACTTATCCGCGCTCGCAGTAC‑GCGAGCGATCAATCACCCTCTTcc                                                                                                                            >  4:67781/1‑149 (MQ=255)
                         gTGGCACTGGGTGCGCATCTGCTGCGCGATGGCGTCGAGGTCATGGAACAGGCTCAAACAGCTCAGGTCATGGGGCACC‑AACCATGAGCCAGCTGCGTTTCAACTTATCCGCGCTCGCAGTAC‑GCGAGCGATCAATCACCCTCTTCCt                                                                                                                           >  2:35431/1‑148 (MQ=255)
                              aCTGGGTGCGCATCTGCTGCGCGATGGCGTCGAGGTCATGGAACAGGCTCAAACAGCTCAGGTCATGGGGCACC‑AACCATGAGCCAGCTGCGTTTCAACTTATCCGCGCTCGCAGTAC‑GCGAGCGATCAATCACCCTCTTCCTGATtgt                                                                                                                     >  8:249051/1‑149 (MQ=255)
                               cTGGGTGCGCATCTGCTGCGCGATGGCGTCGAGGTCATGGAACAGGCTCAAACAGCTCAGGTCATGGGGCACC‑AACCATGAGCCAGCTGCGTTTCAACTTATCCGCGCTCGCAGTAC‑GCGAGCGATCAATCACCCTCTTCCTGATtgtg                                                                                                                    <  1:272462/149‑1 (MQ=255)
                                tGGGTGCGCATCTGCTGCGCGATGGCGTCGAGGTCATGGAACAGGCTCAAACAGCTCAGGTCATGGGGCACC‑AACCATGAGCCAGCTGCGTTTCAACTTATCCGCGCTCGCAGTAC‑GCGAGCGATCAATCACCCTCTTCCTGATTGTGc                                                                                                                   >  7:134226/1‑149 (MQ=255)
                                   gTGCGCATCTGCTGCGCGATGGCGTCGAGGTCATGGAACAGGCTCAAACAGCTCAGGTCATGGGGCACC‑AACCATGAGCCAGCTGCGTTTCAACTTATCCGCGCTCGCAGTAC‑GCGAGCGATCAATCACCCTCTTCCTGATTGTGCTGa                                                                                                                <  6:139561/149‑1 (MQ=255)
                                          tCTGCTGCGCGATGGCGTCGAGGTCATGGAACAGGCTCAAACAGCTCAGGTCATGGGGCACC‑AACCATGAGCCAGCTGCGTTTCAACTTATCCGCGCTCGCAGTAC‑GCGAGCGATCAATCACCCTCTTCCTGATTGTGCTGATCGCCtt                                                                                                         >  3:23386/1‑149 (MQ=255)
                                             gctgcGCGATGGCGTCGAGGTCATGGAACAGGCTCAAACAGCTCAGGTCATGGGGCACC‑AACCATAAGACAGCTGCGTATCAACTTATCCGCGCTCGCAGTAC‑GCGAGCGATCAATCACCCTCTTCCTGATTGTGCTGATCGCCTTCGc                                                                                                      >  5:259174/1‑149 (MQ=255)
                                              ctgcGCGATGGCGTCGAGGTCATGGAACAGGCTCAAACAGCTCAGGTCATGGGGCACC‑AACCATGAGCCAGCTGCGTTTCAACTTATCCGCGCTCGCAGTAC‑GCGAGCGATCAATCACCCTCTTCCTGATTGTGCTGATCGCCTTc                                                                                                        <  4:299237/146‑1 (MQ=255)
                                                 cgcgATGGCGTCGAGGTCATGGAACAGGCTCAAACAGCTCAGGTCATGGGGCACC‑AACCATGAGCCAGCTGCGTTTCAACTTATCCGCGCTCGCAGTAC‑GCGAGCGATCAATCACCCTCTTCCTGATTGTGCTGATCGCCTTCGCCg                                                                                                    <  3:67781/147‑1 (MQ=255)
                                                      tGGCGTCGAGGTCATGGAACAGGCTCAAACAGCTCAGGTCATGGGGCACC‑AACCATGAGCCAGCTGCGTTTCAACTTATCCGCGCTCGCAGTAC‑GCGAGCGATCAATCACCCTCTTCCTGATTGTGCTGATCGCCTTCGCCGGCACGCt                                                                                             >  8:267013/1‑149 (MQ=255)
                                                        gCGTCGAGGTCATGGAACAGGCTCAAACAGCTCAGGTCATGGGGCACC‑AACCATGAGCCAGCTGCGTTTCAACTTATCCGCGCTCGCAGTAC‑GCGAGCGATCAATCACCCTCTTCCTGATTGTGCTGATCGCCTTCGCCGGCACGCTgg                                                                                           >  8:29358/1‑149 (MQ=255)
                                                              aGGTCATGGAACAGGCTCAAACAGCTCAGGTCATGGGGCACC‑AACCATGAGCCAGCTGCGTTTCAACTTATCCGCGCTCGCAGTAC‑GCGAGCGATCAATCACCCTCTTCCTGATTGTGCTGATCGCCTTCGCCGGCACGCTGGCCtttt                                                                                     >  7:80746/1‑149 (MQ=255)
                                                                 tCATGGAACAGGCTCAAACAGCTCAGGTCATGGGGCACC‑AACCATGAGCCAGCTGCGTTTCAACTTATCCGCGCTCGCAGTAC‑GCGAGCGATCAATCACCCTCTTCCTGATTGTGCTGATCGCCTTCGCCGGCACGCTGGCCTTTTTCa                                                                                  <  4:23386/149‑1 (MQ=255)
                                                                 tCATGGAACAGGCTCAAACAGCTCAGGTCATGGGGCACC‑AACCATGAGCCAGCTGCGTTTCAACTTATCCGCGCTCGCAGTAC‑GCGAGCGATCAATCACCCTCATCCTGATTGTGCTGATCGCCTTCGCCGGCACGCTGGCCTTTTTc                                                                                   >  6:51719/1‑148 (MQ=255)
                                                                       aaCAGGCTCAAACAGCTCAGGTCATGGGGCACC‑AACCATGAGCCAGCTGCGTTTCAACTTATCCGCGCTCGCAGTAC‑GCGAGCGATCAATCACCCTCTTCCTGATTGTGCTGATCGCCTTCGCCGGCACGCTGGCCTTTTTCAAGCTgg                                                                            <  8:134226/149‑1 (MQ=255)
                                                                          aGGCTCAAACAGCTCAGGTCATGGGGCACC‑AACCATGAGCCAGCTGCGTTTCAACTTATCCGCGCTCGCAGTAC‑GCGAGCGATCAATCACCCTCTTCCTGATtg                                                                                                                      <  6:130589/104‑1 (MQ=255)
                                                                          aGGCTCAAACAGCTCAGGTCATGGGGCACC‑AACCATGAGCCAGCTGCGTTTCAACTTATCCGCGCTCGCAGTAC‑GCGAGCGATCAATCACCCTCTTCCTGATtg                                                                                                                      >  5:130589/1‑104 (MQ=255)
                                                                          aGGCTCAAACAGCTCAGGTCATGGGGCACC‑AACCATGAGCCAGCTGCGTTTCAACTTATCCGCGCTCGCAGTAC‑GCGAGCGATCAATCACCCTCTTCCTGATTGTGCTGATCGCCTTCGCCGGCACGCTGGCCTTTTTCAAGCTGGGcc                                                                         <  7:29358/149‑1 (MQ=255)
                                                                            gCTCAAACAGCTCAGGTCATGGGGCACC‑AACCATGAGCCAGCTGCGTTTCAACTTATCCGCGCTCGCAGTAC‑GCGAGCGATCAATCACCCTCTTCCTGATTGTGCTGATCGCCTTCGCCGGCACGCTGGCCTTTTTCAAGCTGGGCCGt                                                                       >  2:304636/1‑149 (MQ=255)
                                                                             cTCAAACAGCTCAGGTCATGGGGCACC‑AACCATGAGCCAGCTGCGTTTCAACTTATCCGCGCTCGCAGTAC‑GCGAGCGATCAATCACCCTCTTCCTGATTGTGCTGATCGCCTTCGCCGGCACGCTGGCCTTTTTCAAGCTGGGCCGTg                                                                      <  1:370791/149‑1 (MQ=255)
                                                                                aaaCAGCTCAGGTCATGGGGCACC‑AACCATGAGCCAGCTGCGTTTCAACTTATCCGCGCTCGCAGTAC‑GCGAGCGATCAATCACCCTCTTCCTGATTGTGCTGATCGCCTTCGCCGGCACGCTGGCCTTTTTCAAGCTGGGCCGTg                                                                      >  8:261316/1‑146 (MQ=255)
                                                                                aaaCAGCTCAGGTCATGGGGCACC‑AACCATGAGCCAGCTGCGTTTCAACTTATCCGCGCTCGCAGTAC‑GCGAGCGATCAATCACCCTCTTCCTGATTGTGCTGATCGCCTTCGCCGGCACGCTGGCCTTTTTCAAGCTGGGCCGTg                                                                      >  2:341940/1‑146 (MQ=255)
                                                                                  aCAGCTCAGGTCATGGGGCACC‑AACCATGAGCCAGCTGCGTTTCAACTTATCCGCGCTCGCAGTAC‑GCGAGCGATCAATCACCCTCTTCCTGATTGTGCTGATCGCCTTCGCCGGCACGCTGGCCTTTTTCAAGCTGGGCCGTGCagaa                                                                 <  8:80746/149‑1 (MQ=255)
                                                                                         aGGTCATGGGGCACC‑AACCATGAGCCAGCTGCGTTTCAACTTATCCGCGCTCGCAGTAC‑GCGAGCGATCAATCACCCTCTTCCTGATTGTGCTGATCGCCTTCGCCGGCACGCTGGCCTTTTTCAAGCTgg                                                                            <  2:80471/131‑1 (MQ=255)
                                                                                         aGGTCATGGGGCACC‑AACCATGAGCCAGCTGCGTTTCAACTTATCCGCGCTCGCAGTAC‑GCGAGCGATCAATCACCCTCTTCCTGATTGTGCTGATCGCCTTCGCCGGCACGCTGGCCTTTTTCAAGCTgg                                                                            >  1:80471/1‑131 (MQ=255)
                                                                                         aGGTCATGGGGCACC‑AACCATGAGCCAGCTGCGTTTCAACTTATCCGCGCTCGCAGTAC‑GCGAGCGATCAATCACCCTCTTCCTGATTGTGCTGATCGCCTTCGCCGGCACGCTGGCCTTTTTCAAGCTGGGCCGTGCAGAAGATcc                                                            <  5:367782/147‑1 (MQ=255)
                                                                                           gTCATGGGGCACC‑AACCATGAGCCAGCTGCGTTTCAACTTATCCGCGCTCGCAGTAC‑GCGAGCGATCAATCACCCTCTTCCTGATTGTGCTGATCGCCTTCGCCGGCACGCTGGCCTTTTTCAAGCTGGGCCGTGCAGAAGATCCGccc                                                        <  1:35431/149‑1 (MQ=255)
                                                                                           gTCATGGGGCACC‑AACCATGAGCCAGCTGCGTTTCAACTTATCCGCGCTCGCAGTAC‑GCGAGCGATCAATCACCCTCTTCCTGATTGTGCTGATCGCCTTCGCCGGCACGCTGGCCTTTTTCAAGCTGGGCCGTGCAGAAGATCCGccc                                                        >  2:211601/1‑149 (MQ=255)
                                                                                                       c‑aaccaTGAGCCAGCTGCGTTTCAACTTATCCGCGCTCGCAGTAC‑GCGAGCGATCAATCACCCTCTTCCTGATTGTGCTGATCGCCTTCGCCGGCACGCTGGCCTTTTTCAAGCTGGGCCGTGCAGAAGATCCGCCCTTCACCGTCAAg                                            >  8:7754/1‑149 (MQ=255)
                                                                                                       c‑aaccaTGAGCCAGCTGCGTTTCAACTTATCCGCGCTCGCAGTAC‑GCGAGCGATCAATCACCCTCTTCCTGATTGTGCTGATCGCCTTCGCCGGCACGCTGGCCTTTTTCAAGCTGGGCCGTGCAGAAGATCCGCCCTTCACCGTCAAg                                            >  4:241611/1‑149 (MQ=255)
                                                                                                            caTGAGCCAGCTGCGTTTCAACTTATCCGCGCTCGCAGTAC‑GCGAGCGATCAATCACCCTCTTCCTGATTGTGCTGATCGCCTTCGCCGGCACGCTGGCCTTTTTCAAGCTGGGCCGTGCAGAAGATCCGCCCTTCACCGTCAAGCAGa                                        <  7:249051/149‑1 (MQ=255)
                                                                                                                aGCCAGCTGCGTTTCAACTTATCCGCGCTCGCAGTAC‑GCGAGCGATCAATCACCCTCTTCCTGATTGTGCTGATCGCCTTCGCCGGCACGCTGGCCTTTTTCAAGCTGGGCCGTGCAGAAGATCCGCCCTTCACCGTCAAGCAGATGa                                     <  5:51719/148‑1 (MQ=255)
                                                                                                                  ccAGCTGCGTTTCAACTTATCCGCGCTCGCAGTAC‑GCGAGCGATCAATCACCCTCTTCCTGATTGTGCTGATCGCCTTCGCCGGCACGCTGGCCTTTTTCAAGCTGGGCCGTGCAGAAGATCCGCCCTTCACCGTCAAGCAGATGACGa                                  >  2:363319/1‑149 (MQ=255)
                                                                                                                   cAGCTGCGTTTCAACTTATCCGCGCTCGCATTAC‑GCGTGCGATCAATCACCCTATTCCTGATTGTGCTGATCGCCTTCGCCGGCACGCTGGCCTTTTTCAAGCTGGGCCGTGCAGAAGATCCGCCCTTCACCGTCAAGCAGATGACGa                                  <  1:253118/148‑1 (MQ=255)
                                                                                                                    aGCTGCGTTTCAACTTATCCGCGCTCGCAGTAC‑GCGAGCGATCAATCACCCTCTTCCTGATTGTGCTGATCGCCTTCGCCGGCACGCTGGCCTTTTTCAAGCTGGGCCGTGCAGAAGATCCGCCCTTCACCGTCAAGCAGATGACGAt                                 >  6:149507/1‑148 (MQ=255)
                                                                                                                             tCAACTTATCCGCGCTCGCAGTAC‑GCGAGCGATCAATCACCCTCTTCCTGATTGTGCTGATCGCCTTCGCCGGCACGCTGGCCTTTTTCAAGCTGGGCCGTGCAGAAGATCCGCCCTTCACCGTCAAGCAGATGACGATTATCACTGcc                       >  3:270399/1‑149 (MQ=255)
                                                                                                                               aaCTTATCCGCGCTCGCAGTAC‑GCGAGCGATCAATCACCCTCTTCCTGATTGTGCTGATCGCCTTCGCCGGCACGCTGGCCTTTTTCAAGCTGGGCCGTGCAGAAGATCCGCCCTTCACCGTCAAGCAGATGACGATTATCACTGCCTg                     >  4:303136/1‑149 (MQ=255)
                                                                                                                                        tcgcTCGC‑GTACTGCGAGCGATCAATCACCCTCTTCCTGATTGTGCTGATCGCCTTCGCCGGCACGCTGGCCTTTTTCAAGCTGGGCCGTGCAGAAGATCCGCCCTTCACCGTCAAGCAGATGa                                     >  8:173247/2‑124 (MQ=255)
                                                                                                                                        tcgcTCGC‑GTACTGCGAGCGATCAATCACCCTCTTCCTGATTGTGCTGATCGCCTTCGCCGGCACGCTGGCCTTTTTCAAGCTGGGCCGTGCAGAAGATCCGCCCTTCACCGTCAAGCAGATGa                                     <  7:173247/123‑1 (MQ=255)
                                                                                                                                        gcgcTCGCAGTAC‑GCGAGCGATCAATCACCCTCTTCCTGATTGTGCTGATCGCCTTCGCCGGCACGCTGGCCTTTTTCAAGCTGGGCCGTGCAGAAGATCCGCCCTTCACCGTCAAGCAGATGACGa                                  <  3:268478/127‑1 (MQ=255)
                                                                                                                                        gcgcTCGCAGTAC‑GCGAGCGATCAATCACCCTCTTCCTGATTGTGCTGATCGCCTTCGCCGGCACGCTGGCCTTTTTCAAGCTGGGCCGTGCAGAAGATCCGCCCTTCACCGTCAAGCAGATGACGa                                  >  4:268478/1‑127 (MQ=255)
                                                                                                                                          gcTCGCAGTAC‑GCGAGCGATCAATCACCCTCTTCCTGATTGTGCTGATCGCCTTCGCCGGCACGCTGGCCTTTTTCAAGCTGGGCCGTGCAGAAGATCCGCCCTTCACCGTCAAGCAGATGACGATTATCACTGCCTGGCCCGGCGCCa          >  8:111445/1‑149 (MQ=255)
                                                                                                                                                  tAC‑GCGAGCGATCAATCACCCTCTTCCTGATTGTGCTGATCGCCTTCGCCGGCACGCTGGCCTTTTTCAAGCTGGGCCGTGCAGAAGATCCGCCCTTCACCGTCAAGCAGATGACGATTATCACTGCCTGGCCCGGCGCCACTGCGCAg  <  3:318051/149‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                  
TGACCTGCACGCTGGCGATCGGATCGTGGCACTGGGTGCGCATCTGCTGCGCGATGGCGTCGAGGTCATGGAACAGGCTCAAACAGCTCAGGTCATGGGGCACC‑AACCATGAGCCAGCTGCGTTTCAACTTATCCGCGCTCGCAGTAC‑GCGAGCGATCAATCACCCTCTTCCTGATTGTGCTGATCGCCTTCGCCGGCACGCTGGCCTTTTTCAAGCTGGGCCGTGCAGAAGATCCGCCCTTCACCGTCAAGCAGATGACGATTATCACTGCCTGGCCCGGCGCCACTGCGCAG  >  NC_002947/3734996‑3735289

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑