Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	4,758,147	A→G	9.8%	intergenic (+7/+14)	ompQ → / ← pvdP	outer membrane pyoverdine efflux protein/pyoverdine biosynthesis‑like protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	4,758,147	0	A	G	9.8%	167.8 / 6.8	61	intergenic (+7/+14)	ompQ/pvdP	outer membrane pyoverdine efflux protein/pyoverdine biosynthesis‑like protein
Reads supporting (aligned to +/- strand):  ref base A (26/29);  new base G (3/3);  total (29/32)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CTGACCGTGCTCGACACCCAGCGCAGCCTGTACCAGGCCCAGGACCAGCAGGCCCGCCTGCAGCTAAGCCAACTGCAGGCCAGCGTGGCCCTTTACAAAGCCTTGGGCGGTGGCTGGCAAGCGGATAGCCCGACGCGCTAGGGCGTCAGGCTATCTGGTGTTCACGACCGCTGCCTGACCTTCAGGTGGCGAGCATACCAGGGGCGGCGCGGCACTTTGCCTACCAGCCCGTCAAAGCGCTCCTCGTCGCTGTAAATCAGGCGCAAGGCCAGCTTCATGGTTTCCGGATCCATCTC  >  NC_002947/4758000‑4758295                                                                                                                                                    |                                                                                                                                                     cTGACCGTGCTCGACACCCAGCGCAGCCTGTACCAGGCCCAGGACCAGCAGACCCGCCTGCAGCTAAGCCAACTGCAGGCCAGCGTGGCCCTTTACAAAGCCTTGGGCGGTGGCTGGCAAGCGGATAGCCCGACGCGCTAGGGCGTCAg                                                                                                                                                     >  1:92561/1‑149 (MQ=255)      cGTGCTCGACACCCAGCGCAGCCTGTACCAGGCCCAGGACCAGCAGGCCCGCCTGCAGCTAAGCCAACTGCAGGCCAGCGTGGCCCTTTACAAAGCCTTGGGCGGTGGCTGGCAAGCGGATAGCCCGACGCGCTAGGGCGTCAGGCTAt                                                                                                                                                >  3:270607/1‑149 (MQ=255)       gTGCTCGACACCCAGCGCAGCCTGTACCAGGCCCAGGACCAGCAGGCCCGCCTGCAGCTAAGCCAACTGCAGGCCAGCGTGGCCCTTTACAAAGCCTTGGGCGGTGGCTGGCAAGCGGATAGCCCGACGCGCTAGGGCGTCAGGCTATc                                                                                                                                               <  2:92561/149‑1 (MQ=255)         gCTCGACACCCAGCGCAGCCTGTACCAGGCCCAGGACCAGCAGGCCCGCCTGCAGCTAAGCCAACTGCAGGCCAGCGTGGCCCTTTACAAAGCCTTGGGCGGTGGCTGGCAAGCGGATAGCCCGACGCGCTAGGGCGTCAGGCTATc                                                                                                                                               <  5:233011/147‑1 (MQ=255)         gCTCGACACCCAGCGCAGCCTGTACCAGGCCCAGGACCAGCAGGCCCGCCTGCAGCTAAGCCAACTGCAGGCCAGCGTGGCCCTTTACAAAGCCTTGGGCGGTGGCTGGCAAGCGGATAGCCCGACGCGCTAGGGCGTCAGGCTATc                                                                                                                                               >  6:233011/1‑147 (MQ=255)           tCGACACCCAGCGCAGCCTGTACCAGGCCCAGGACCAGCAGGCCCGCCTGCAGCTAAGCCAACTGCAGGCCAGCGTGGCCCTTTACAAAGCCTTGGGCGGTGGCTGGCAAGCGGATAGCCCGACGCGCTAGGGCGTCAGGCTATCTGgt                                                                                                                                           >  2:329109/1‑149 (MQ=255)             gACACCCAGCGCAGCCTGTACCAGGCCCAGGACCAGCAGGCCCGCCTGCAGCTAAGCCAACTGCAGGCCAGCGTGGCCCTTTACAAAGCCTTGGGCGGTGGCTGGCAAGCGGATAGCCCGACGCGCTAGGGCGTCAGGCTATCTGgtgt                                                                                                                                         >  8:6614/1‑149 (MQ=255)             gACACCCAGCGCAGCCTGTACCAGGCCCAGGACCAGCAGGCCCGCCTGCAGCTAAGCCAACTGCAGGCCAGCGTGGCCCTTTACAAAGCCTTGGGCGGTGGCTGGCAAGCGGATAGCCCGACGCGCTAGGGCGTCAGGCTATCTGgtgt                                                                                                                                         >  2:274557/1‑149 (MQ=255)                acCCAGCGCAGCCTGTACCAGGCCCAGGACCAGCAGGCCCGCCTGCAGCTAAGCCAACTGCAGGCCAGCGTGGCCCTTTACAAAGCCTTGGGCGGTGGCTGGCAAGCGGATAGCCTGACGCCCTAGCGCGTCGGGc                                                                                                                                                   >  1:113350/1‑136 (MQ=255)                acCCAGCGCAGCCTGTACCAGGCCCAGGACCAGCAGGCCCGCCTGCAGCTAAGCCAACTGCAGGCCAGCGTGGCCCTTTACAAAGCCTTGGGCGGTGGCTGGCAAGCGGATAGCCTGACGCCCTAGCGCGTCGGGc                                                                                                                                                   <  2:113350/136‑1 (MQ=255)                 cccAGCGCAGCCTGTACCAGGCCCAGGACCAGCAGGCCCGCCTGCAGCTAAGCCAACTGCAGGCCAGCGTGGCCCTTTACAAAGCCTTGGGCGGTGGCTGGCAAGCGGATAGCCCGACGCGCTAGGGCGTCAGGCTATCTGGTGTTCAc                                                                                                                                     >  2:358212/1‑149 (MQ=255)                  ccAGCGCAGCCTGTACCAGGCCCAGGACCAGCAGGCCCGCCTGCAGCTAAGCCAACTGCAGGCCAGCGTGGCCCTTTACAAAGCCTTGGGCGGTGGCTGGCAAGCGGATAGCCCGACGCGCTAGGGCGTCAGGCTATCTGGTGTTCAc                                                                                                                                     >  2:367510/1‑148 (MQ=255)                  ccAGCGCAGCCTGTACCAGGCCCAGGACCAGCAGGCCCGCCTGCAGCTAAGCCAACTGCAGGCCAGCGTGGCCCTTTACAAAGCCTTGGGCGGTGGCTGGCAAGCGGATAGCCCGACGCGCTAGGGCGTCAGGCTATCTGGTGTTCACg                                                                                                                                    >  7:269353/1‑149 (MQ=255)                       gcAGCCTGTACCAGGCCCAGGACCAGCAGGCCCGCCTGCAGCTAAGCCAACTGCAGGCCAGCGTGGCCCTTTACTAAGCCTTGGGCGGTGGCTGGCAAGCGGATAGCCCGACGCGCTAGGGCGTCAGGCTATCTGGTGTTCACGACCGc                                                                                                                               <  4:37153/149‑1 (MQ=255)                        cAGCCTGTACCAGGCCCAGGACCAGCAGGCCCGCCTGCAGCTAAGCCAACTGCAGGCCAGCGTGGCCCTTTACAAAGCCTTGGGCGGTGGCTGGCAAGCGGATAGCCTGACGCCCTAGCGCGTCGGGCt                                                                                                                                                  >  7:79554/1‑129 (MQ=255)                        cAGCCTGTACCAGGCCCAGGACCAGCAGGCCCGCCTGCAGCTAAGCCAACTGCAGGCCAGCGTGGCCCTTTACAAAGCCTTGGGCGGTGGCTGGCAAGCGGATAGCCTGACGCCCTAGCGCGTCGGGCt                                                                                                                                                  <  8:79554/129‑1 (MQ=255)                        cAGCCTGTACCAGGCCCAAGACCAGCAGGCCCGCCTGCAGCTAAGCCAACTGCAGGCCAGCGTGGCCCTTTACAAAGCCTTGGGCGGTGGCTGGCAAGCGGATAGCCCGACGCGCTAGGGCGTCAGGCTATCTGGTGTTCACGACCGCt                                                                                                                              <  4:19154/149‑1 (MQ=255)                            cTGTACCAGGCCCAGGACCAGCAGGCCCGCCTGCAGCTAAGCCAACTGCAGGCCAGCGTGGCCCTTTACAAAGCCTTGGGCGGTGGCTGGCAAGCGGATAGCCCGACGCGCTAGGGCGTCAGGCTATCTGGTGTTCACGACCGCTGCCt                                                                                                                          <  7:191071/149‑1 (MQ=255)                               tACCAGGCCCAGGACCAGCAGGCCCGCCTGCAGCTAAGCCAACTGCAGGCCAGCGTGGCCCTTTACAAAGCCTTGGGCGGTGGCTGGCAAGCGGATAGCCCGACGCGCTAGGGCGTCAGGCTATCTGGTGTTCACGACCGCTGCCTg                                                                                                                         <  1:367510/147‑1 (MQ=255)                               tACCAGGCCCAGGACCAGCAGGCCCGCCTGCAGCTAAGCCAACTGCAGGCCAGCGTGGCCCTTTACAAAGCCTTGGGCGGTGGCTGGCAAGCGGATAGCCCGACGCGCTAGGGCGTCAGGCTATCTGGTGTTCACGACCGCTGCCTGa                                                                                                                        <  3:365866/148‑1 (MQ=255)                                 ccAGGCCCAGGACCAGCAGGCCCGCCTGCAGCTAAGCCAACTGCAGGCCAGCGTGGCCCTTTACAAAGCCTTGGGCGGTGGCTGGCAAGCGGATAGCCCGACGCGCTAGGGCGTCAGGCTATCTGGTGTTCACGACCGCTGCCTGACCt                                                                                                                     >  8:326625/1‑149 (MQ=255)                                       ccAGGACCAGCAGGCCCGCCTGCAGCTAAGCCAACTGCAGGCCAGCGTGGCCCTTTACAAAGCCTTGGGCGGTGGCTGGCAAGCGGATAGCCCGACGCGCTAGGGCGTCAGGCTATCTGGTGTTCACGACCGCTGCCTGACCTTCAGGt                                                                                                               >  4:110649/1‑149 (MQ=255)                                        cAGGACCAGCAGGCCCGCCTGCAGCTAAGCCAACTGCAGGCCAGCGTGGCCCTTTACAAAGCCTTGGGCGGTGGCTGGCAAGCGGATAGCCCGACGCGCTAGGGCGTCAGGCTATCTGGTGTTCACGACCGCTGCCTGACCTTCAGGTg                                                                                                              <  8:269353/149‑1 (MQ=255)                                              cagcagGCCCGCCTGCAGCTAAGCCAACTGCAGGCCAGCGTGGCCCTTTACAAAGCCTTGGGCGGTGGCTGGCAAGCGGATAGCCCGACGCGCTAGGGCGTCAGGCTATCTGGTGTTCACGACCGCTGCCTGACCTTCAGGTGGCGAg                                                                                                         >  5:379245/1‑148 (MQ=255)                                                  agGCCCGCCTGCAGCTAAGCCAACTGCAGGCCAGCGTGGCCCTTTACAAAGCCTTGGGCGGTGGCTGGCAAGCGGATAGCCCGACGCGCTAGGGCGTCAGGCTATCTGGTGTTCACGACCGCTGCCTGACCTTCAGGTGGCGAGCATAc                                                                                                    >  3:129800/1‑149 (MQ=255)                                                       cGCCTGCAGCTAAGCCAACTGCAGGCCAGCGTGGCCCTTTACAAAGCCTTGGGCGGTGGCTGGCAAGCGGATAGCCCGACGCGCTAGGGCGTCAGGCTATCTGGTGTTCACGACCGCTGCCTGACCTTCAGGTGGCGAGCATACCAggg                                                                                               <  2:242184/149‑1 (MQ=255)                                                         ccTGCAGCTAAGCCAACTGCAGGCCAGCGTGGCCCTTTACAAAGCCTTGGGCGGTGGCTGGCAAGCGGATAGCCCGACGCGCTAGGGCGTCAGGCTATCTGGTGTTCACGACCGCTGCCTGACCTTCAGGTGGCGAGCATACCAGGggc                                                                                             >  3:126994/1‑149 (MQ=255)                                                           tGCAGCTAAGCCAACTGCAGGCCAGCGTGGCCCTTTACAAAGCCTTGGGCGGTGGCTGGCAAGCGGATAGCCCGACGCGCTAGGGCGTCAGGCTATCTGGTGTTCACGACCGCTGCCTGACCTTCAGGTGGCGAGCATACCAGGggcgg                                                                                           >  3:18078/1‑149 (MQ=255)                                                              aGCTAAGCCAACTGCAGGCCAGCGTGGCCCTTTACAAAGCCTTGGGCGGTGGCTGGCAAGCGGATAGCCCGACGCGCTAGGGCGTCAGGCTATCTGGTGTTCACGACCGCTGCCTGACCTTCAGGTGGCGAGCATACCAGGGgcggcgc                                                                                        <  1:329109/149‑1 (MQ=255)                                                                   aGCCAACTGCAGGCCAGCGTGGCCCTTTACAAAGCCTTGGGCGGTGGCTGGCAAGCGGATAGCCCGACGCGCTAGGGCGTCAGGCTATCTGGTGTTCACGACCGCTGCCTGACCTTCAGGTGGCGAGCATACCAGGGGCGGCGCGGCAc                                                                                   >  1:260304/1‑149 (MQ=255)                                                                     ccAACTGCAGGCCAGCGTGGCCCTTTACAAAGCCTTGGGCGGTGGCTGGCAAGCGGATAGCCCGACGCGCTAGGGCGTCAGGCTATCTGGTGTTCACGACCGCTGCCTGACCTTCAGGTGGCGAGCATACCAGGGGCGGCGCGGCACtt                                                                                 >  4:189962/1‑149 (MQ=255)                                                                            cAGGCCAGCGTGGCCCTTTACAAAGCCTTGGGCGGTGGCTGGCAAGCGGATAGCCCGACGCGCTAGGGCGTCAGGCTATCTGGTGTTCACGACCGCTGCCTGACCTTCAGGTGGCGAGCATACCAGGGGCGGCGCGGCACTTTGCCTAc                                                                          <  1:274557/149‑1 (MQ=255)                                                                            cAGGCCAGCGTGGCCCTTTACAAAGCCTTGGGCGGTGGCTGGCAAGCGGATAGCCCGACGCGCTAGGGCGTCAGGCTATCTGGTGTTCACGACCGCTGCCTGACCTTCAGGTGGCGAGCATACCAGGGGCGGCGCGGCACTTTGCCTAc                                                                          <  7:6614/149‑1 (MQ=255)                                                                                 cAGCGTGGCCCTTTACAAAGCCTTGGGCGGTGGCTGGCAAGCGGATAGCCCGACGCGCTAGGGCGTCAGGCTATCTGGTGTTCACGACCGCTGCCTGACCTTCAGGTGGCGAGCATACCAGGGGCGGCGCGGCACTTTGCCTACCAGcc                                                                     >  5:324854/1‑149 (MQ=255)                                                                                 cAGCGTGGCCCTTTACAAAGCCTTGGGCGGTGGCTGGCAAGCGGATAGCCCGACGCGCTAGGGCGTCAGGCTATCTGGTGTTCACGACCGCTGCCTGACCTTCAGGTGGCGAGCATACCAGGGGCGGCGCGGCACTTTGCCTACCAGcc                                                                     >  3:161513/1‑149 (MQ=255)                                                                                  aGCGTGGCCCTTTACAAAGCCTTGGGCGGTGGCTGGCAAGCGGATAGCCCGACGCGCTAGGGCGTCAGGCTATCTGGTGTTCACGACCGCTGCCTGACCTTCAGGTGGCGAGCATACCAGGGGCGGCGCGGCACTTTGCCTACCAGccc                                                                    <  4:270607/149‑1 (MQ=255)                                                                                    cGTGGCCCTTTACACAGCCTTGGGCGGTGGCTGGCAAGCGGATAGCCCGACGCGCTAGGGCGTCAGGCTATCTGGTGTTCACGACCGCTGCCTGACCTTCAGGTGGCGAGCATACCAGGGGCGGCGCGGCACTTTGCCTACCAGCCCGt                                                                  <  4:196139/149‑1 (MQ=255)                                                                                     gTGGCCCTTTACAAAGCCTTGGGCGGTGGCTGGCAAGCGGATAGCCCGACGCGCTAGGGCGTCAGGCTATCTGGTGTTCACGACCGCTGCCTGACCTTCAGGTGGCGAGCATACCAGGGGCGGCGCGGCACTTTGCCTACCAGCCCGTc                                                                 <  6:282424/149‑1 (MQ=255)                                                                                          ccTTTACAAAGCCTTGGGCGGTGGCTGGCAAGCGGATAGCCCGACGCGCTAGGGCGTCAGGCTATCTGGTGTTCACGACCGCTGCCTGACCTTCAGGTGGCGAGCATACCAGGGGCGGCGCGGCACTTTGCCTACCAGCCCGTCAAAgc                                                            >  5:89554/1‑149 (MQ=255)                                                                                               aCAAAGCCTTGGGCGGTGGCTGGCAAGCGGATAGCCCGACGCGCTAGGGCGTCAGGCTATCTGGTGTTCACGACCGCTGCCTGACCTTCAGGTGGCGAGCATACCAGGGGCGGCGCGGCACTTTGCCTACCAGCCCGTCAAAGCGctc                                                        >  2:43428/1‑148 (MQ=255)                                                                                                cAAAGCCTTGGGCGGTGGCTGGCAAGCGGATAGCCCGACGCGCTAGGGCGTCAGGCTATCTGGTGTTCACGACCGCTGCCTGACCTTCAGGTGGCGAGCATACCAGGGGCGGCGCGGCACTTTGCCTACCAGCCCGTCAAAGCGctcc                                                       <  4:126994/148‑1 (MQ=255)                                                                                                 aaaGCCTTGGGCGGTGGCTGGCAAGCGGATAGCCCGACGCGCTAGGGCGTCAGGCTATCTGGTGTTCACGACCGCTGCCTGACCTTCAGGTGGCGAGCATACCAGGGGCGGCGCGGCACTTTGCCTACCAGCCCGTCAAAGCGctcctc                                                     <  7:326625/149‑1 (MQ=255)                                                                                                     ccTTGGGCGGTGGCTGGCAAGCGGATAGCCCGACGCGCTAGGGCGTCAGGCTATCTGGTGTTCACGACCGCTGCCTGACCTTCAGGTGGCGAGCATACCAGGGGCGGCGCGGCACTTTGCCTACCAGCCCGTCAAAGCGCTCCtcgtcg                                                 >  4:24254/1‑149 (MQ=255)                                                                                                          ggCGGTGGCTGGCAAGCGGATAGCCCGACGCGCTAGGGCGTCAGGCTATCTGGTGTTCACGACCGCTGCCTGACCTTCAGGTGGCGAGCATACCAGGGGCGGCGCGGCACTTTGCCTACCAGCCCGTCAAAGCGCTCCTCGTCGCTGt                                             <  4:129800/148‑1 (MQ=255)                                                                                                                 gctggcAAGCGGATAGCCCGACGCGCTAGGGCGTCAGGCTATCTGGTGTTCACGACCGCTGCCTGACCTTCAGGTGGCGAGCATACCAGGGGCGGCGCGGCACTTTGCCTACCAGCCCGTCAAAGCGCTCCTCGTCGCTGTAAATCAg                                      <  2:260304/148‑1 (MQ=255)                                                                                                                  ctggcAAGCGGATAGCCCGACGCGCTAGGGCGTCAGGCTATCTGGTGTTCACGACCGCTGCCTGACCTTCAGGTGGCGAGCATACCAGGGGCGGCGCGGCACTTTGCCTACCAGCCCGTCAAAGCGCTCCTCGTCGCTGTAAATCAGgc                                    <  4:18078/149‑1 (MQ=255)                                                                                                                  ctggcAAGCGGATAGCCCGACGCGCTAGGGCGTCAGGCTATCTGGTGTTCACGACCGCTGCCTGACCTTCAGGTGGCGAGCATACCAGGGGCGGCGCGGCACTTTGCCTACCAGCCCGTCAAAGCGCTCCTCGTCGCTGTAAATCAGgc                                    <  6:89554/149‑1 (MQ=255)                                                                                                                      cAAGCGGATAGCCCGACGCGCTAGGGCGTCAGGCTATCTGGTGTTCACGACCGCTGCCTGACCTTCAGGTGGCGAGCATACCAGGGGCGGCGCGGCACTTTGCCTACCAGCCCGTCAAAGCGCTCCTCGTCGCTGTAAATCAGGCGCaa                                <  3:110649/149‑1 (MQ=255)                                                                                                                       aaGCGAATAGCCCGACGCGCTAGGGCGGCAGGCTATCTGGTGTTCACGACCGCTGCCTGACCTTCAGGTGGCGAGCATACCAGGGGCGGCGCGGCACTTTGCCTACCAGCCCGTCAAAg                                                             <  6:71031/119‑1 (MQ=255)                                                                                                                                 cctgACGCCCTAGCGCGTCGGGCTATCTGGTGTTCACGACCGCTGCCTGACCTTCAGGTGGCGAGCATACCAGGGGCGGCGCGGCACTTTGCCTACCAGCCCGTCAAAgcgc                                                          <  3:360954/109‑1 (MQ=255)                                                                                                                                 cctgACGCCCTAGCGCGTCGGGCTATCTGGTGTTCACGACCGCTGCCTGACCTTCAGGTGGCGAGCATACCAGGGGCGGCGCGGCACTTTGCCTACCAGCCCGTCAAAgcgc                                                          >  4:360954/4‑112 (MQ=255)                                                                                                                                  ccGACGCGCTAGGGCGTCAGGCTATCTGGTGTTCACGACCGCTGCCTGACCTTCAGGTGGCGAGCATACCAGGGGCGGCGCGGCACTTTGCCTACCAGCCCGTCAAAGCGCTCCTCGTCGCTGTAAATCAGGCGCAAGGCCAGCTTCAt                    >  2:98651/1‑149 (MQ=255)                                                                                                                                       gcgcTAGGGCGTCAGGCTATCTGGTGTTCACGACCGCTGCCTGACCTTCAGGTGGCGAGCATACCAGGGGCGGCGCGGCACTTTGCCTACCAGCCCGTCAAAGCGCTCCTCGTCGCTGTAAATCAGGCGCAAg                               >  2:98904/1‑133 (MQ=255)                                                                                                                                       gcgcTAGGGCGTCAGGCTATCTGGTGTTCACGACCGCTGCCTGACCTTCAGGTGGCGAGCATACCAGGGGCGGCGCGGCACTTTGCCTACCAGCCCGTCAAAGCGCTCCTCGTCGCTGTAAATCAGGCGCAAg                               <  1:98904/133‑1 (MQ=255)                                                                                                                                        cgcTAGGGCGTCAGGCTATCTGGTGTTCACGACCGCTGCCTGACCTTCAGGTGGCGAGCATACCAGGGGCGGCGCGGCACTTTGCCTACCAGCCCGTCAAAGCGCTCCTCGTCGCTGTAAATCAGGCGCAAGGCCAGCTTCATGGTTTc              <  3:189962/149‑1 (MQ=255)                                                                                                                                              ggCGTCAGGCTATCTGGTGTTCACGACCGCTGCCTGACCTTCAGGTGGCGAGCATACCAGGGGCGGCGCGGCACTTTGCCTACCAGCCCGTCAAAGCGCTCCTCGTCGCTGTAAATCAGGCGCAAGGCCAGCTTCATGGt                 <  4:219612/140‑1 (MQ=255)                                                                                                                                              ggCGTCAGGCTATCTGGTGTTCACGACCGCTGCCTGACCTTCAGGTGGCGAGCATACCAGGGGCGGCGCGGCACTTTGCCTACCAGCCCGTCAAAGCGCTCCTCGTCGCTGTAAATCAGGCGCAAGGCCAGCTTCATGGt                 >  3:219612/1‑140 (MQ=255)                                                                                                                                              ggCGTCAGGCTATCTGGTGTTCACGACCGCTGCCTGACCTTCAGGTGGCGAGCATACCAGGGGCGGCGCGGCACTTTGCCTACCAGCCCGTCAAAGCGCTCCTCGTCGCTGTAAATCAGGCGCAAGGCCAGCTTCATGGTTTCCGGATc        <  6:324854/149‑1 (MQ=255)                                                                                                                                              ggCGTCAGGCTATCTGGTGTTCACGACCGCTGCCTGACCTTCAGGTGGCGAGCATACCAGGGGCGGCGCGGCACTTTGCCTACCAGCCCGTCAAAGCGCTCCTCGTCGCTGTAAATCAGGCGCAAGGCCAGCTTCATGGTTTCCGGATc        <  4:161513/149‑1 (MQ=255)                                                                                                                                               gCGTCAGGCTATCTGGTGTTCACGACCGCTGCCTGACCTTCAGGTGGCGAGCATACCAGGGGCGGCGCGGCACTTTGCCTACCAGCCCGTCAAAGCGCTCCTCGTCGCTGTAAATCAGGCGCAAGGCCAGCTTCATGGTTTCCGGATcc       <  1:358212/149‑1 (MQ=255)                                                                                                                                                cGTCAGGCTATCTGGTGTTCACGACCGCTGCCTGACCTTCAGGTGGCGAGCATACCAGGGGCGGCGCGGCACTTTGCCTACCAGCCCGTCAAAGCGCTCCTCGTCGCTGTAAATCAGGCGCAAGGCCAGCTTCATGGTTTCCGGATCCa      >  2:216568/1‑149 (MQ=255)                                                                                                                                                    aGGCTATCTGGTGTTCACGACCGCTGCCTGACCTTCAGGTGGCGAGCATACCAGGGGCGGCGCGGCACTTTGCCTACCAGCCCGTCAAAGCGCTCCTCGTCGCTGTAAATCAGGCGCAAGGCCAGCTTCATGGTTTCCGGATCCAtctc  >  6:309167/1‑149 (MQ=255)                                                                                                                                                    |                                                                                                                                                     CTGACCGTGCTCGACACCCAGCGCAGCCTGTACCAGGCCCAGGACCAGCAGGCCCGCCTGCAGCTAAGCCAACTGCAGGCCAGCGTGGCCCTTTACAAAGCCTTGGGCGGTGGCTGGCAAGCGGATAGCCCGACGCGCTAGGGCGTCAGGCTATCTGGTGTTCACGACCGCTGCCTGACCTTCAGGTGGCGAGCATACCAGGGGCGGCGCGGCACTTTGCCTACCAGCCCGTCAAAGCGCTCCTCGTCGCTGTAAATCAGGCGCAAGGCCAGCTTCATGGTTTCCGGATCCATCTC  >  NC_002947/4758000‑4758295

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑