Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,772,083 A→G 7.5% V2395A (GTC→GCC)  pvdD ← non‑ribosomal peptide synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,772,0830AG 7.5% 146.9 / 3.0 53V2395A (GTC→GCC) pvdDnon‑ribosomal peptide synthetase
Reads supporting (aligned to +/- strand):  ref base A (23/26);  new base G (2/2);  total (25/28)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.00e-01

CCGGGCGCCCCGCCACCGTGGCGCCAAAGCTGACACTGCGCTGGCCGCTGTAGCGGCTGAGCAGCAACAACCAGGCGCCCTGCACCACGGTGTTGAGGGTGATGCGCTGGGATTGTGCGAAGGCCTTGAACGTCTCGGTCTGCGCGACACTGAAACGGCTGTAGAGGGCCTGGTGGCCGCTGCCGCGCTCACCGCTGGCGAAGGCATCGGCCAGGTAGGTCGGCTCCTGCAGGCTGGCCAGCTGCTGCTTCCAGAACGTTTCGCTGTGGCGCGGATCCTGCTGGCGCAGCCAGCC  >  NC_002947/4771937‑4772231
                                                                                                                                                  |                                                                                                                                                    
ccGGGCGCCCCGCCACCGTGGCGCCAAAGCTGACACTGCGCTGGCCGCTGTAGCGGCTGAGCAGCAACAACCAGGCGCCCTGCACCACGGTGTTGAGGGTGATGCGCTGGGATTGTGCGAAGGCCTTGAACGTCTCGGTCTGCGCGaca                                                                                                                                                    >  3:186042/1‑149 (MQ=255)
      gcCCCGCCACCGTGGCGCCAAAGCTGACACTGCGCTGGCCGCTGTAGCGGCTGAGCAGCAACAACCAGGCGCCCTGCACCACGGTGTTGAGGGTGATGCGCTGGGATTGTGCGAAGGCCTTGAACGTCTCGGTCTGCGCGACACTGaaa                                                                                                                                              >  8:351587/1‑149 (MQ=255)
         ccGCCACCGTGGCGCCAAAGCTGACACTGCGCTGGCCGCTGTAGCGGCTGAGCAGCAACAACCAGGCGCCCTGCACCACGGTGTTGAGGGTGATGCGCTGGGATTGTGCGAAGGCCTTGAACGTCTCGGTCTGCGCGACACTGAAACgg                                                                                                                                           <  4:186042/149‑1 (MQ=255)
           gCCACCGTGGCGCCAAAGCTGACACTGCGCTGGCCGCTGTAGCGGCTGAGCAGCAACAACCAGGCGCCCTGCACCACGGTGTTGAGGGTGATGCGCTGGGATTGTGCGAAGGCCTTGAACGTCTCGGTCTGCGCGACACTGAAACGGCt                                                                                                                                         <  5:196531/149‑1 (MQ=255)
            ccACCGTGGCGCCAAAGCTGACACTGCGCTGGCCGCTGTAGCGGCTGAGCAGCAACAACCAGGCGCCCTGCACCACGGTGTTGAGGGTGATGCGCTGGGATTGTGCGAAGGCCTTGAACGTCTCGGTCTGCGCGACACTGAAACGGCTg                                                                                                                                        <  8:339064/149‑1 (MQ=255)
            ccACCGTGGCGCCAAAGCTGACACTGCGCTGGCCGCTGTAGCGGCTGAGCAGCAACAACCAGGCGCCCTGCACCACGGTGTTGAGGGTGATGCGCTGGGATTGTGCGAAGGCCTTGAACGTCTCGGTCTGCGCGACACTGAAACGGCTg                                                                                                                                        >  2:1078/1‑149 (MQ=255)
             cACCGTGGCGCCAAAGCTGACACTGCGCTGGCCGCTGTAGCGGCTGAGCAGCAACAACCAGGCGCCCTGCACCACGGTGTTGAGGGTGATGCGCTGGGATTGTGCGAAGGCCTTGAACGTCTCGGTCTGCGCGACACTGAAACGGCTGt                                                                                                                                       <  8:139215/149‑1 (MQ=255)
              aCCGTGGCGCCAAAGCTGACAATGCGCTGGCCGCTGTAGCGGCTGAGCAGCAAGAAGCTGGCGGCCTGCACCACGGTTTTGAGGGTGATGCGCTGTGATTGTGCGTAGGCCTTGAACGTCTCGGTCTGCGCGACACTGAAACGGCTGTa                                                                                                                                      <  5:9568/149‑1 (MQ=255)
                  tGGCGCCAAAGCTGACACTGCGCTGGCCGCTGTAGCGGCTGAGCAGCAACAACCAGGCGCCCTGCACCACGGTGTTGAGGGTGATGCGCTGGGATTGTGCGAAGGCCTTGAACGTCTCGGTCTGCGCGACACTGAAACGGCTGTAGAgg                                                                                                                                  >  2:338600/1‑149 (MQ=255)
                        cAAAGCTGACACTGCGCTGGCCGCTGTAGCGGCTGAGCAGCAACAACCAGGCGCCCTGCACCACGGTGTTGAGGGTGATGCGCTGGGATTGTGCGAAGGCCTTGAACGTCTCGGTCTGCGCGACACTGAAACGGCTGTAGAGGGCCtgg                                                                                                                            >  2:209594/1‑149 (MQ=255)
                        cAAAGCTGACACTGCGCTGGCCGCTGTAGCGGCTGAGCAGCAACAACCAGGCGCCCTGCACCACGGTGTTGAGGGTGATGCGCTGGGATTGTGCGAAGGCCTTGAACGTCTCGGTCTGCGCGACACTGAAACGGCTGTAGAGGGCCt                                                                                                                              <  6:328839/147‑1 (MQ=255)
                        cAAAGCTGACACTGCGCTGGCCGCTGTAGCGGCTGAGCAGCAACAACCAGGCGCCCTGCACCACGGTGTTGAGGGTGATGCGCTGGGATTGTGCGAAGGCCTTGAACGTCTCGGTCTGCGCGACACTGAAACGGCTGTAGAGGGCCt                                                                                                                              >  5:328839/1‑147 (MQ=255)
                          aaGCTGACACTGCGCTGGCCGCTGTAGCGGCTGAGCAGCAACAACCAGGCGCCCTGCACCACGGTGTTGAGGGTGATGCGCTGGGATTGTGCGAAGGCCTTGAACGTCTCGGTCTGCGCGACACTGAAACGGCTGTAGAGGGCCtggtg                                                                                                                          <  5:156903/149‑1 (MQ=255)
                           aGCTGACACTGCGCTGGCCGCTGTAGCGGCTGAGCAGCAACAACCAGGCGCCCTGCACCACGGTGTTGAGGGTGATGCGCTGGGATTGTGCGAAGGCCTTGAACGTCTCGGTCTGCGCGACACTGAAACGGCTGTAGAGGGCCtggtgg                                                                                                                         >  4:58980/1‑149 (MQ=255)
                            gCTGACACTGCGCTGGCCGCTGTAGCGGCTGAGCAGCAACAACCAGGCGCCCTGCACCACGGTGTTGAGGGTGATGCGCTGGGATTGTGCGAAGGCCTTGAACGTCTCGGTCTGCGCGACACTGAAACGGCTGTAGAGGGCCTGGTGGc                                                                                                                        <  1:1078/149‑1 (MQ=255)
                                acacTGCGCTGGCCGCTGTAGCGGCTGAGCAGCAACAACCAGGCGCCCTGCACCACGGTGTTGAGGGTGATGCGCTGGGATTGTGCGAAGGCCTTGAACGTCTCGGTCTGCGCGACACTGAAACGGCTGt                                                                                                                                       <  3:148318/130‑1 (MQ=255)
                                acacTGCGCTGGCCGCTGTAGCGGCTGAGCAGCAACAACCAGGCGCCCTGCACCACGGTGTTGAGGGTGATGCGCTGGGATTGTGCGAAGGCCTTGAACGTCTCGGTCTGCGCGACACTGAAACGGCTGt                                                                                                                                       >  4:148318/1‑130 (MQ=255)
                                             cGCTGTAGCGGCTGAGCAGCAACAACCAGGCGCCCTGCACCACGGTGTTGAGGGTGATGCGCTGGGATTGTGCGAAGGCCTTGAACGTCTCGGTCTGCGCGACACTGAAACGGCTGTAGAGGGCCTGGTGGCCGCTGCCGCGCTCACCg                                                                                                       >  3:200854/1‑149 (MQ=255)
                                                      ggCTGAGCAGCAACAACCAGGCGCCCTGCACCACGGTGTTGAGGGTGATGCGCTGGGATTGTGCGAAGGCCTTGAACGTCTCGGTCTGCGCGACACTGAAACGGCTGTAGAGGGCCTGGTGGCCGCTGCCGCGCTCACCGCTGGCGAAg                                                                                              <  3:58980/149‑1 (MQ=255)
                                                         tGAGCAGCAACAACCAGGCGCCCTGCACCACGGTGTTGAGGGTGATGCGCTGGGATTGTGCGAAGGCCTTGAACGTCTCGGTCTGCGCGACACTGAAACGGCTGTAGAGGGCCTGGTGGCCGCTGCCGCGCTCACCGCTGGCGAAGGCa                                                                                           >  7:77914/1‑149 (MQ=255)
                                                           agcagcAACAACCAGGCGCCTTGCACCACGGTGTTGAGGGTGATGCGCTGGGATTGTGCGAAGGCCTTGAACGTCTCGGTCTGCGCGACACTGAAACGGCTGTAGAGGGCCTGGTGGCCGCTGCCGCGCTCACCGCTGGCGAAGGCATc                                                                                         <  4:255735/149‑1 (MQ=255)
                                                           agcagcAACAACCAGGCGCCCTGCACCACGGTGTTGAGGGTGATGCGCTGGGATTGTGCGAAGGCCTTGAACGTCTCGGTCTGCGCGACACTGAAACGGCTGTAGAGGGCCTGGTGGCCGCTGCCGCGCTCACCGCTGGCGAAGGCATc                                                                                         <  4:200854/149‑1 (MQ=255)
                                                             cagcaACAACCAGGCGCCCTGCACCACGGTGTTGAGGGTGATGCGCTGGGATTGTGCGAAGGCCTTGAACGTCTCGGTCTGCGCGACACTGAAACGGCTGTAGAGGGCCTGGTGGCCGCTGCCGCGCTCACCGCTGGCGAAGGCATCgg                                                                                       >  8:241874/1‑149 (MQ=255)
                                                                caacaaCCAGGCGCCCTGCACCACGGTGTTGAGGGTGATGCGCTGGGATTGTGCGAAGGCCTTGAACGTCTCGGTCTGCGCGACACTGAAACGGCTGTAGAGGGCCTGGTGGCCGCTGCCGCGCTCACCGCTGGCGAAGGCATCGGCCa                                                                                    <  8:68886/149‑1 (MQ=255)
                                                                 aacaacCAGGCGCCCTGCACCACGGTGTTGAGGGTGATGCGCTGGGATTGTGCGAAGGCCTTGAACGTCTCGGTCTGCGCGACACTGAAACGGCTGTAGAGGGCCTGGTGGCCGCTGCCGCGCTCACCGCTGGCGAAGGCATCGGCCag                                                                                   <  3:289304/149‑1 (MQ=255)
                                                                  acaacCAGGCGCCCTGCACCACGGTGTTGAGGGTGATGCGCTGGGATTGTGCGAAGGCCTTGAACGTCTCGGTCTGCGCGACACTGAAACGGCTGTAGAGGGCCTGGTGGCCGCTGCCGCGCTCACCGCTGGCGAAGGCATCGGCCagg                                                                                  >  3:303993/1‑149 (MQ=255)
                                                                      ccAGGCGCCCTGCACCACGGTGTTGAGGGTGATGCGCTGGGATTGTGCGAAGGCCTTGAACGTCTCGGTCTGCGCGACACTGAAACGGCTGTAGAGGGCCTGGTGGCCGCTGCCGCGCTCACCGCTGGCGAAGGCATCGGCCaggtagg                                                                              >  5:232049/1‑149 (MQ=255)
                                                                       cAGGCGCCCTGCACCACGGTGTTGAGGGTGATGCGCTGGGATTGTGCGAAGGCCTTGAACGTCTCGGTCTGCGCGGCACTGAAACGGCTGTAGAGGGCCtggtgg                                                                                                                         <  8:134572/105‑1 (MQ=255)
                                                                       cAGGCGCCCTGCACCACGGTGTTGAGGGTGATGCGCTGGGATTGTGCGAAGGCCTTGAACGTCTCGGTCTGCGCGGCACTGAAACGGCTGTAGAGGGCCtggtgg                                                                                                                         >  7:134572/1‑105 (MQ=255)
                                                                       cAGGCGCCCTGCACCACGGTGTTGAGGGTGATGCGCTGGGATTGTGCGAAGGCCTTGAACGTCTCGGTCTGCGCGACACTGAAACGGCTGTAGAGGGCCTGGTGGCCGCTGCCGCGCTCACCGCTGGCGAAGGCATCGGCCaggtaggt                                                                             <  4:303993/149‑1 (MQ=255)
                                                                           cgcCCTGCACCACGGTGTTGAGGGTGATGCGCTGGGATTGTGCGAAGGCCTTGAACGTCTCGGTCTGCGCGACACTGAAACGGCTGTAGAGGGCCTGGTGGCCGCTGCCGCGCTCACCGCTGGCGAAGGCATCGGCCAGGTAGGTCGGc                                                                         <  7:351587/149‑1 (MQ=255)
                                                                               cTGCACCACGGTGTTGAGGGTGATGCGCTGGGATTGTGCGAAGGCCTTGAACGTCTCGGTCTGCGCGACACTGAAACGGCTGTAGAGGGCCTGGTGGCCGCTGCCGCGCTCACCGCTGGCGAAGGCATCGGCCag                                                                                   <  4:56718/135‑1 (MQ=255)
                                                                               cTGCACCACGGTGTTGAGGGTGATGCGCTGGGATTGTGCGAAGGCCTTGAACGTCTCGGTCTGCGCGACACTGAAACGGCTGTAGAGGGCCTGGTGGCCGCTGCCGCGCTCACCGCTGGCGAAGGCATCGGCCag                                                                                   >  3:56718/1‑135 (MQ=255)
                                                                                    ccacGGTGTTGAGGGTGATGCGCTGGGATTGTGCGAAGGCCTTGAACGTCTCGGTCTGCGCGACACTGAAACGGCTGTAGAGGGCCTGGTGGCCGCTGCCGCGCTCACCGCTGGCGAAGGCATCGGCCagg                                                                                  <  2:252152/131‑1 (MQ=255)
                                                                                    ccacGGTGTTGAGGGTGATGCGCTGGGATTGTGCGAAGGCCTTGAACGTCTCGGTCTGCGCGACACTGAAACGGCTGTAGAGGGCCTGGTGGCCGCTGCCGCGCTCACCGCTGGCGAAGGCATCGGCCagg                                                                                  >  1:252152/1‑131 (MQ=255)
                                                                                                 ggTGATGCGCTGGGATTGTGCGAAGGCCTTGAACGTCTCGGTCTGCGCGACACTGAAACGGCTGTAGAGGGCCTGGTGGCCGCTGCCGCGCTCACCGCTGGCGAAGGCATCGGCCAGGTAGGTCGGCTCCTGCAGGCTGGCCAgctgct                                                   <  7:241874/149‑1 (MQ=255)
                                                                                                 ggTGATGCGCTGGGATTGTGCGAAGGCCTTGAACGTCTCGGTCTGCGCGACACTGAAACGGCTGTAGAGGGCCTGGTGGCCGCTGCCGCGCTCACCGCTGGCGAAGGCATCGGCCAGGTAGGTCGGCTCCTGCAGGCTGGCCAgctgct                                                   >  3:121042/1‑149 (MQ=255)
                                                                                                    gATGCGCTGGGATTGTGCGAAGGCCTTGAACGTCTCGGTCTGCGCGGCACTGAAACGGCTGTAGAGGGCCTGGTGGCCGCTGCCGCTCTCACCGCTGGCGAAGGCATCGGCCAGGTAGGTCGGCTCCTGCAGGCTGGc                                                           <  5:259495/138‑1 (MQ=255)
                                                                                                    gATGCGCTGGGATTGTGCGAAGGCCTTGAACGTCTCGGTCTGCGCGGCACTGAAACGGCTGTAGAGGGCCTGGTGGCCGCTGCCGCGCTCACCGCTGGCGAAGGCATCGGCCAGGTAGGTCGGCTCCTGCAGGATGGc                                                           >  6:259495/1‑138 (MQ=255)
                                                                                                        cgcTGGGATTGTGCGAAGGCCTTGAACGTCTCGGTCTGCGCGACACTGAAACGGCTGTAGAGGGCCTGGTGGCCGCTGCCGCGCTCACCGCTGGCGAAGGCATCGGCCAGGTAGGTCGGCTCCTGCAGGCTGGCCAGCTGCTGCTTCCa                                            <  6:232049/149‑1 (MQ=255)
                                                                                                         gcTGGGATTGTGCGAAGGCCTTGAACGTCTCGGTCTGCGCGACACTGAAACGGCTGTAGAGGGCCTGGTGGCCGCTGCCGCGCTCACCGCTGGCGAAGGCATCGGCCAGGTAGGTCGGCTCCTGCAGGCTGGCCAGCTGCTGCTTc                                              >  8:326653/1‑146 (MQ=255)
                                                                                                            gggATTGTGCGAAGGCCTTGAACGTCTCGGTCTGCGCGACACTGAAACGGCTGTAGAGGGCCTGGTGGCCGCTGCCGCGCTCACCGCTGGCGAAGGCATCGGCCAGGTAGGTCGGCTCCTGCAGGCTGGCCAGCTGCTGCTTCCAGAAc                                        <  7:131660/149‑1 (MQ=255)
                                                                                                             ggATTGTGCGAAGGCCTTGAACGTCTCGGTCTGCGCGACACTGAAACGGCTGTAGAGGGCCTGGTGGCCGCTGCCGCGCTCACCGCTGGCGAAGGCATCGGCCAGGTAGGTCGGCTCCTGCAGGCTGGCCAGCTGCTGCTTCCAGaa                                         <  1:338600/147‑1 (MQ=255)
                                                                                                               aTTGTGCGAAGGCCTTGAACGTCTCGGTCTGCGCGACACTGAAACGGCTGTAGAGGGCCTGGTGGCCGCTGCCGCGCTCACCGCTGGCGAAGGCATCGGCCAGGTAGGTCGGCTCCTGCAGGCTGGCCAGCTGCTGCTTCCAGAACGtt                                     >  7:282730/1‑149 (MQ=255)
                                                                                                                      gAAGGCCTTGAACGTCTCGGTCTGCGCGACACTGAAACGGCTGTAGAGGGCCTGGTGGCCGCTGCCGCGCTCACCGCTGGCGAAGGCATCGGCCAGGTAGGTCGGCTCCTGCAGGCTGGCCAGCTGCTGCTTCCAGAACGTTTCGCtgt                              >  7:207816/1‑149 (MQ=255)
                                                                                                                        aGGCCTTGAACGTCTCGGTCTGCGCGACACTGAAACGGCTGTAGAGGGCCTGGTGGCCGCTGCCGCGCTCACCGCTGGCGAAGGCATCGGCCAGGTAGGTCGGCTCCTGCAGGCTGGCCAGCTGCTGCTTCCAGAACGTTTCGCTGTgg                            <  4:121042/149‑1 (MQ=255)
                                                                                                                             ttGAACGTCTCGGTCTGCGCGACACTGAAACGGCTGTAGAGGGCCTGGTGGCCGCTGCCGCGCTCACCGCTGGCGAAGGCATCGGCCAGGTAGGTCGGCTCCTGCAGGCTGGCCAGCTGCTGCTTCCAGAACGTTTCGCTGTGGcgcg                        >  8:277144/1‑148 (MQ=255)
                                                                                                                             ttGAACGTCTCGGTCTGCGCGACACTGAAACGGCTGTAGAGGGCCTGGTGGCCGCTGCCGCGCTCACCGCTGGCGAAGGCATCGGCCAGGTAGGTCGGCTCCTGCAGGCTGGCCAGCTGCTGCTTCCAGAACGTTTCGCTGTGGcgcg                        >  4:354017/1‑148 (MQ=255)
                                                                                                                             ttGAACGTCTCGGTCTGCGCGACACTGAAACGGCTGTAGAGGGCCTGGTGGCCGCTGCCGCGCTCACCGCTGGCGAAGGCATCGGCCAGGTAGCTCGGCTCCTGCAGGCTGGCCAGCTGCTGCTTCCAGAACGTTTCGCTGTGGcgcg                        >  6:274596/1‑148 (MQ=255)
                                                                                                                                   gTCTCGGTCTGCGCGACACTGAAACGGCTGTAGAGGGCCTGGTGGCCGCTGCCGCGCTCACCGCTGGCGAAGGCATCGGCCAGGTAGGTCGGCTCCTGCAGTCTGGCCAGCTGCTGCTTCCAGAACGTTTCGCTGTGGCGCGGATCctg                 >  4:77641/1‑149 (MQ=255)
                                                                                                                                    tctcGGTCTGCGCGACACTGAAACGGCTGTAGAGGGCCTGGTGGCCGCTGCCGCGCTCACCGCTGGCGAAGGCATCGGCCAGGTAGGTCGGCTCCTGCAGGCTGGCCAGCTGCTGCTTCCAGAACGTTTCGCTGTGGCGCGGATCctgc                <  7:277144/149‑1 (MQ=255)
                                                                                                                                    tctcGGTCTGCGCGACACTGAAACGGCTGTAGAGGGCCTGGTGGCCGCTGCCGCGCTCACCGCTGGCGAAGGCATCGGCCAGGTAGGTCGGCTCCTGCAGGCTGGCCAGCTGCTGCTTCCAGAACGTTTCGCTGTGGCGCGGATCctgc                <  3:354017/149‑1 (MQ=255)
                                                                                                                                        ggTCTGCGCGACACTGAAACGGCTGTAGAGGGCCTGGTGGCCGCTGCCGCGCTCACCGCTGGCGAAGGCATCGGCCAGGTAGGTCGGCTCCTGCAGGCTGGCCAGCTGCTGCTTCCAGAACGTTTCGCTGTGGCGCGGATCctgctg              <  1:129156/147‑1 (MQ=255)
                                                                                                                                        ggTCTGCGCGACACTGAAACGGCTGTAGAGGGCCTGGTGGCCGCTGCCGCGCTCACCGCTGGCGAAGGCATCGGCCAGGTAGGTCGGCTCCTGCAGGCTGGCCAGCTGCTGCTTCCAGAACGTTTCGCTGTGGCGCGGATCCTGCTGgc            >  5:154725/1‑149 (MQ=255)
                                                                                                                                                  acacTGAAACGGCTGTATAGTGCCTGGTTGCCGCTGCCGCGCTCACCGATGGCGAAGGCATCTGCCAGGTAGGTCGTCTGCTGCAGGCTGGCCAGCTGCTGCTTGCAGAACGTTTCGCTGTGGCGCGGATCCTGCTGGCTgcgccagca  <  8:77914/149‑2 (MQ=255)
                                                                                                                                                  |                                                                                                                                                    
CCGGGCGCCCCGCCACCGTGGCGCCAAAGCTGACACTGCGCTGGCCGCTGTAGCGGCTGAGCAGCAACAACCAGGCGCCCTGCACCACGGTGTTGAGGGTGATGCGCTGGGATTGTGCGAAGGCCTTGAACGTCTCGGTCTGCGCGACACTGAAACGGCTGTAGAGGGCCTGGTGGCCGCTGCCGCGCTCACCGCTGGCGAAGGCATCGGCCAGGTAGGTCGGCTCCTGCAGGCTGGCCAGCTGCTGCTTCCAGAACGTTTCGCTGTGGCGCGGATCCTGCTGGCGCAGCCAGCC  >  NC_002947/4771937‑4772231

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑