Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,782,608 A→G 7.7% L1569P (CTC→CCC)  pvdJ ← non‑ribosomal peptide synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,782,6080AG 7.7% 149.1 / 2.6 52L1569P (CTC→CCC) pvdJnon‑ribosomal peptide synthetase
Reads supporting (aligned to +/- strand):  ref base A (25/23);  new base G (2/2);  total (27/25)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01

CGCTCACCCAGGTCCCAGCAAAACGAGGTGCGCAGCACGTCATGGCGCGCAATGACCGCTTTCCAGGCTTCGCTGAACGCGCCGACATCAATGGCGTGGTCGACGCGGTAGCAATACTGCATCAGATAGATGCCCGAACCCGGTTCGAGCAAGGTGTGCATCAGCAGCCCTTCCTGCATCGGCGACAACGGATAGATGTCTTCGATATCGCGCGCGGCCACCGGCAGGCGATCCAGTTGCTGCTGGCTCAGCCCGGCCAGTGGGAAGTCGCTTGGCGTAATGCCTTGCGCCTCAGG  >  NC_002947/4782461‑4782756
                                                                                                                                                   |                                                                                                                                                    
cgcTCACCCAGGTCCCAGCAAAACGAGGTGCGCAGCACGTCATGGCGCGCAATGACCGCTTTCCAGGCTTCGCTGAACGCGCCGACATCAATGGCGTGGTCGACGCGGTAGCAATACTGCATCAGATAGATGCCCGAACCCGGTTCGAg                                                                                                                                                     <  3:254079/149‑1 (MQ=255)
      cccAGGTCCCAGCAAAACGAGGTGCGCAGCACGTCATGGCGCGCAATGCCCGCTTTCCAGGCTTCGCTGAACGCGCCGACATCAATGGCGTGGTCGACGCGGTAGCAATACTGCATCAGATAGATGCCCGAACCCGGTTCGAGCAAGgt                                                                                                                                               >  7:168877/1‑149 (MQ=255)
          ggTCCCAGCAAAACGAGGTGCGCAGCACGTCATGGCGCGCAATGACCGCTTTCCAGGCTTCGCTGAACGCGCCGACATCAATGGCGTGGTCGACGCGGTAGCAATACTGCATCAGATAGATGCCCGAACCCGGTTCGAGCAAGGTGTGc                                                                                                                                           <  7:187489/149‑1 (MQ=255)
            tCCCAGCAAAACGAGGTGCGCAGCACGTCATGGCGCGCAATGACCGCTTTCCAGGCTTCGCTGAACGCGCCGACATCAATGGCGTGGTCGACGCGGTAGCAATACCGCATCAGATAGATGCCCGAACCCGGTTCGAGCAAGGTGTGCAt                                                                                                                                         >  8:74862/1‑149 (MQ=255)
                 gCAAAACGAGGTGCGCAGCACGTCATGGCGCGCAATGACCGCTTTCCAGGCTTCGCTGAACGCGCCGACATCAATGGCGTGGTCGACGCGGTAGCAATACTGCATCAGATAGATGCCCGAACCCGGTTCGAGCAAGGTGTGCATcagca                                                                                                                                    <  1:142019/149‑1 (MQ=255)
                 gCAAAACGAGGTGCGCAGCACGTCATGGCGCGCAATGACCGCTTTCCAGGCTTCGCTGAACGCGCCGACATCAATGGCGTGGTCGACGCGGTAGCAATACTGCATCAGATAGATGCCCGAACCCGGTTCGAGCAAGGTGTGCATcagca                                                                                                                                    <  4:4458/149‑1 (MQ=255)
                     aaCGAGGTGCGCAGCACGTCATGGCGCGCAATGACCGCTTTCCAGGCTTCGCTGAACGCGCCGACATCAATGGCGTGGTCGACGCGGTAGCAATACTGCATCAGATAGATGCCCGAACCCGGTTCGAGCAAGGTGTGCATCAGCAGccc                                                                                                                                >  3:38562/1‑149 (MQ=255)
                             gcgcAGCACGTCATGGCGCGCAATGACCGCTTTCCAGGCTTCGCTGAACGCGCCGACATCAATGGCGTGGTCGACGCGGTAGCAATACTGCATCAGATAGATGCCCGAACCCGGTTCGAGCAAGGTGTGCATCAGCAGCCCTTCCTGCa                                                                                                                        >  7:327280/1‑149 (MQ=255)
                                 agcaCGTCATGGCGCGCAATGACCGCTTTCCAGGCTTCGCTGAACGCGCCGACATCAATGGCGTGGTCGACGCGGTAGCAATACTGCATCAGATAGATGCCCGAACCCGGTTCGAGCAAGGTGTGCATCAGCAGCCCTTCCTGCATCgg                                                                                                                    <  2:38704/149‑1 (MQ=255)
                                 agcaCGTCATGGCGCGCAATGACCGCTTTCCAGGCTTCGCTGAACGCGCCGACATCAATGGCGTGGTCGACGCGGTAGCAATACTGCATCAGATAGATGCCCGAACCCGGTTCGAGCAAGGTGTGAATCAGCAGCCCTTCCTGCATCgg                                                                                                                    >  3:326163/1‑149 (MQ=255)
                                       tCATGGCGCGCAATGACCGCTTTCCAGGCTTCGCTGAACGCGCCGACATCAATGGCGTGGTCGACGCGGTAGCAATACTGCATCAGATAGATGCCCGAACCCGGTTCGAGCAAGGTGTGCATCAGCAGCCCTTCCTGCATCGGCGACaa                                                                                                              <  8:327280/149‑1 (MQ=255)
                                              gcgcAATGACCGCTTTCCAGGCTTCGCTGAACGCGCCGACATCAATGGCGTGGTCGACGCGGTAGCAATACTGCATCAGATAGATGCCCGAACCCGGTTCGAGCAAGGTGTGCATCAGCAGCCCTTCCTGCATCGGCGACAACGGa                                                                                                          >  3:161113/1‑146 (MQ=255)
                                              gcgcAATGACCGCTTTCCAGGCTTCGCTGAACGCGCCGACATCAATGGCGTGGTCGACGCGGTAGCAATACCGCATCAGATAGATGCCCGAACCCGGTTCGAGCAAGGTGTGCATCAGCAGCCCTTCCTGCATCGGCGACAACGGATAg                                                                                                       <  7:74862/149‑1 (MQ=255)
                                                gcAATGACCGCTTTCCAGGCTTCGCTGAACGCGCCGACATCAATGGCGTGGTCGACGCGGTAGCAATACTGCATCAGATAGATGCCCGAACCCGGTTCGAGCAAGGTGTGCATCAGCAGCCCTTCCTGCATCGGCGACAACGGATAGAt                                                                                                     <  6:364364/149‑1 (MQ=255)
                                                 cAATGACCGCTTTCCAGGCTTCGCTGAACGCGCCGACATCAATGGCGTGGTCGACGCGGTAGCAATACTGCATCAGATAGATGCCCGAACCCGGTTCGAGCAAGGTGTGCATCAGCAGCCCTGCCTGCATCGGCGACAACGgagagaag                                                                                                    >  8:302432/1‑147 (MQ=255)
                                                     gACCGCTTTCCAGGCTTCGCTGAACGCGCCGACATCAATGGCGTGGTCGACGCGGTAGCAATACTGCATCAGATAGATGCCCGAACCCGGTTCGAGCAAGGTGTGCATCAGCAGCCCTTCCTGCATCGGCGa                                                                                                                 >  2:86085/1‑132 (MQ=255)
                                                     gACCGCTTTCCAGGCTTCGCTGAACGCGCCGACATCAATGGCGTGGTCGACGCGGTAGCAATACTGCATCAGATAGATGCCCGAACCCGGCTCGAGCAAGGTGTGCATCAGCAGCCCTTCCTGCATCGGCGa                                                                                                                 <  1:86085/132‑1 (MQ=255)
                                                       ccGCTTTCCAGGCTTCGCTGAACGCGCCGACATCAATGGCGTGGTCGACGCGGTAGCAATACTGCATCAGATAGATGCCCGAACCCGGTTCGAGCAAGGTGTGCATCAGCAGCCCTTCCTGCATCGGCGACAACGGATAGATGTCTTc                                                                                               >  6:77908/1‑148 (MQ=255)
                                                        cGCTTTCCAGGCTTCGCTGAACGCGCCGACATCAATGGCGTGGTCGACGCGGTAGCAATACTGCATCAGATAGATGCCCGAACCCGGTTCGAGCAAGGTGTGCATCAGCAGCCCTTCCTGCATCGGCGACAACGGATAGATGTCTTCGa                                                                                             >  7:142800/1‑149 (MQ=255)
                                                        cGCTTTCCAGGCTTCGCTGAACGCGCCGACATCAATGGCGTGGTCGACGCGGTAGCAATACTGCATCAGATAGATGCCCGAACCCGGTTCGAGCAAGGTGTGCATCAGCAGCCCTTCCTGCATCGGCGACAACGGATAGATGTCTTCGa                                                                                             >  7:137773/1‑149 (MQ=255)
                                                        cGCTTTCCAGGCTTCGCTGAACGCGCCGACATCAATGGCGTGGTCGACGCGGTAGCAATACTGCATCAGATAGATGCCCGAACCCGGTTCGAGCAAGGTGTGCATCAGCAGCCCTTCCTGCATCGGCGACAACGGATAGATGTCTTCGa                                                                                             >  5:359847/1‑149 (MQ=255)
                                                                       gCTGAACGCGCCGACATCAATGGCGTGGTCGACGCGGTAGCAATACTGCATCAGATAGATGCCCGAACCCGGTTCGAGCAAGGTGTGCATCAGCAGCCCTTCCTGCATCGGCGACAACGGATAGATGTCTTCGATATCGCGCGCGGcc                                                                               <  5:77908/148‑1 (MQ=255)
                                                                           aaCGCGCCGACATCAATGGCGTGGTCGACGCGGTAGCAATACTGCATCAGATAGATGCCCGAACCCGGTTCGAGCAAGGTGTGCATCAGCAGCCCTTCCTGCATCGGCGACAACGGATAGATGTCTTCGATATCGCGCGCGGCCAcc                                                                            <  6:359847/147‑1 (MQ=255)
                                                                                                     gACGCGGTAGCAATACTGCATCAGATAGATGCCCGAACCCGGTTCGAGCAAGGTGTGCATCAGCAGCCCTTCCTGCATCGGCGACAACGGATAGATGTCTTCGATATCGCGCGCGGCCACCGGCAGGCGATCCAGTTGCTGCTGGCt                                                  >  8:71673/1‑147 (MQ=255)
                                                                                                       cgcgGTAGCAATACTGCATCAGATAGATGCCCGAACCCGGTTCGAGCAAGGTGTGCATCAGCAGCCCTTCCTGCATCGGCGACAACGGATAGATGTCTTCGATATCGCGCGCGGCCACCGGCAGGCGATCCAGTTGCTGCTGGCTCAGc                                              <  4:161113/149‑1 (MQ=255)
                                                                                                          ggTAGCAATACTGCATCAGATAGATGCCCGAACCCGGTTCGAGCAAGGTGTGCATCAGCAGCCCTTCCTGCATCGGCGACAACGGATAGATGTCTTCGATATCGCGCGCGGCCACCGGCAGGCGATCCAGTTGCTGCTGGCTCAGCCCg                                           >  7:294289/1‑149 (MQ=255)
                                                                                                            tAGCAATACTGCATCAGATAGATGCCCGAACCCGGTTCGAGCAAGGTGTGCATCAGCAGCCCTTCCTGCATCGGCGACAACGGATAGATGTCTTCGATATCGCGCGCGGCCACCGGCAGGCGATCCAGTTGCTGCTGGCTCAGCCCGGc                                         >  7:48889/1‑149 (MQ=255)
                                                                                                                  tACTGCATCAGATAGATGCCCGAACCCGGTTCGAGCAAGGTGTGCATCAGCAGCCCTTCCTGCATCGGCGACAACGGATAGATGTCTTCGATATCGCGCGCGGCCACCGGCAGGCGATCCAGTTGCTGCTGGCTCAGCCCGGCCAGTgg                                   >  6:66677/1‑149 (MQ=255)
                                                                                                                      gCATCAGATAGATGCCCGAACCCGGTTCGAGCAAGGTGTGCATCAGCAGCCCTTCCTGCATCGGCGACAACGGATAGATGTCTTCGATATCGCGCGCGGCCACCGGCAGGCGATCCAGTTGCTGCTGGCTCAGCCCGGCCAGt                                     >  5:39407/1‑143 (MQ=255)
                                                                                                                      gCATCAGATAGATGCCCGAACCCGGTTCGAGCAAGGTGTGCATCAGCAGCCCTTCCTGCATCGGCGACAACGGATAGATGTCTTCGATATCGCGCGCGGCCACCGGCAGGCGATCCAGTTGCTGCTGGCTCAGCCCGGCCAGt                                     <  6:39407/143‑1 (MQ=255)
                                                                                                                        aTCAGATAGATGCCCGAACCCGGTTCGAGCAAGGTGTGCATCAGCAGCCCTTCCTGCATCGGCGACAACGGATAGATGTCTTCGATATCGCGCGCGGCCACCGGCAGGCGATCCAGTTGCTGCTGGCACAGCCCGGCCAGTGGGAAGTc                             <  4:38562/149‑1 (MQ=255)
                                                                                                                          cAGATAGATGCCCGAACCCGGTTCGAGCAAGGTGTGCATCAGCAGCCCTTCCTGCATCGGCGACAACGGATAGATGTCTTCGATATCGCGCGCGGCCACCGGCAGGCGATc                                                                 >  7:342457/1‑111 (MQ=255)
                                                                                                                            gatagatGCCCGAACCCGGTTCGAGCAAGGTGTGCATCAGCAGCCCTTCCTGCATCGGCGACAACGGATAGATGTCTTCGATATCGCGCGCGGCCACCGGCAGGCGATACAGTTGCTGCTGGCTCAGCCCGGCCAGTGGGAAGTCGCtt                         >  3:121172/1‑149 (MQ=255)
                                                                                                                            gatagatGCCCGAACCCGGTTCGAGCAAGGTGTGCATCAGCAGCCCTTCCTGCATCGGCGACAACGGATAGATGTCTTCGATATCCCGCGCGGCCACCGGCAGGCGATCCAGTTGCTGCTGGCTCAGCCCGGCCAGTGGGAAGTCGCtt                         >  6:161373/1‑149 (MQ=255)
                                                                                                                             caccttGCTCGAACCGGGTTCGGGCAAGGTGTGCATCAGCAGCCCTTCCTGCATCGGCGACAACGGATAGATGTCTTCGATATCGCGCGCGGCCACCGGCAGGCg                                                                    <  4:145399/100‑1 (MQ=255)
                                                                                                                             caccttGCTCGAACCGGGTTCGGGCAAGGTGTGCATCAGCAGCCCTTCCTGCATCGGCGACAACGGATAGATGTCTTCGATATCGCGCGCGGCCACCGGCAGGCg                                                                    >  3:145399/6‑105 (MQ=255)
                                                                                                                                cttGCTCGAACCGGGTTCGGGCAAGGTGTGCATCAGCAGCCCTTCCTGCATCGGCGACAACGGATAGATGTCTTCGATATCGCGCGCGGCCACCGGCAGGCGATCCAGTTGCTGCTGGCt                                                  >  6:82496/3‑120 (MQ=255)
                                                                                                                                cttGCTCGAACCGGGTTCGGGCAAGGTGTGCATCAGCAGCCCTTCCTGCATCGGCGACAACGGATAGATGTCTTCGATATCGCGCGCGGCCACCGGCAGGCGATCCAGTTGCTGCTGGCt                                                  <  5:82496/118‑1 (MQ=255)
                                                                                                                                   gCCCGAACCCGGTTCGAGCAAGGTGTGCATCAGCAGCCCTTCCTGCATCGGCGACAACGGATAGATGTCTTCGATATCGCGCGCGGCCACCGGCAGGCGATCCAGTTGCTGCTGGCTCAGCCCGGCCAGTGGGAAGTCGCTTg                        <  3:48178/143‑1 (MQ=255)
                                                                                                                                   gCCCGAACCCGGTTCGAGCAAGGTGTGCATCAGCAGCCCTTCCTGCATCGGCGACAACGGATAGATGACTTCGATATCGCGCGCGGCCACCGGCAGGCGATCCAGTTGCTGCTGGCTCAGCCCGGCCAGTGGGAAGTCGCTTg                        >  4:48178/1‑143 (MQ=255)
                                                                                                                                     ccGAACCCGGTTCGAGCAAGGTGTGCATCAGCAGCCCTTCCTGCATCGGCGACAACGGATAGATGTCTTCGATATCGCGCGCGGCCACCGGCAGGCGATCCAGTTGCTGCTGGCTCAGCCCGGCCAGTGGGAAGTCGCTTGGCGTAATg                >  5:120079/1‑149 (MQ=255)
                                                                                                                                     ccGAACCCGGTTCGAGCAAGGTGTGAATCAGCAGCCCTTCCTGCATCGGCGACAACGGATAGATGTCTTCGATATCGCGCGCGGCCACCGGCAGGCGATCCAGTTGCTGCTGGCTCAGCCCGGCCAGTGGGAAGTCGCTTGGCGTAATg                <  4:326163/149‑1 (MQ=255)
                                                                                                                                          cccGGTTCGAGCAAGGTGTGCATCAGCAGCCCTTCCTGCATCGGCGACAACGGATAGATGTCTTCGATATCGCGCGCGGCCACCGGCAGGCGATCCAGTTGCTGCTGGCTCAGCCCGGCCAGTGGGAAGTCGCTTGGCGTAATGCCTTg           <  8:137773/149‑1 (MQ=255)
                                                                                                                                          cccGGTTCGAGCAAGGTGTGCATCAGCAGCCCTTCCTGCATCGGCGACAACGGATAGATGTCTTCGATATCGCGCGCGGCCACCGGCAGGCGATCCAGTTGCTGCTGGCTCAGCCCGGCCAGTGGGAAGTCGCTTGGCGTAATGCCTTg           <  8:142800/149‑1 (MQ=255)
                                                                                                                                           ccGGTTCGAGCAAGGTGTGCATCAGCAGCCCTTCCTGCATCGGCGACAACGGATAGATGTCTTCGATATCGCGCGCGGCCACCGGCAGGCGATCCAGTTGCTGCTGGCTCAGCCCGGCCAGTGGGAAGt                              <  1:227461/129‑1 (MQ=255)
                                                                                                                                           ccGGTTCGAGCAAGGTGTGCATCAGCAGCCCTTCCTGCATCGGCGACAACGGATAGATGTCTTCGATATCGCGCGCGGCCACCGGCAGGCGATCCAGTTGCTGCTGGCTCAGCCCGGCCAGTGGGAAGt                              >  2:227461/1‑129 (MQ=255)
                                                                                                                                             ggTTCGAGCAAGGTGTGCATCAGCAGCCCTTCCTGCATCGGCGACAACGGATAGATGTCTTCGATATCGCGCGCGGCCACCGGCAGGCGATCCAGTTGCTGCTGGCTCAGCCCGGCCAGTGGGAAGTCGCTTGGCGTAATGCCTTgcgc        <  6:120079/149‑1 (MQ=255)
                                                                                                                                             ggTTCGAGCAAGGTGTGCATCAGCAGCCCTTCCTGCATCGGCGACAACGGATAGATGTCTTCGATATCGCGCGCGGCCACCGGCAGGCGATCCAGTTGCTGCTGGCTCAGCCCGGCCAGTGGGAAGTCGCTTGGCGTAATGCCTTgc          >  6:163714/1‑147 (MQ=255)
                                                                                                                                             ggTTCGAGCAAGGTGTGCATCAGCAGCCCTTCCTGCATCGGCGACAACGGATAGATGTCTTCGATATCGCGCGCGGCCACCGGCAGGCGATCCAGTTGCTGCTGGCTCAGCCCGGCCAGTGGGAAGTCGCTTGGCGTAATGCCTTgc          <  5:163714/147‑1 (MQ=255)
                                                                                                                                               ttCGAGCAAGGTGTGCATCAGCAGCCCTTCCTGCATCGGCGACAACGGATAGATGTCTTCGATATCGCGCGCGGCCACCGGCAGGCGATCCAGTTGCTGCTGGCTCAGCCCGGCCAGTGGGAAGTCGCTTGGCGTAATGCCTTGCGCCt      >  2:154683/1‑149 (MQ=255)
                                                                                                                                                  gAGCAAGGTGTGCATCAGCAGCCCTTCCTGCATCGGCGACAACGGATAGATGTCTTCGATATCGCGCGCGGCCACCGGCAGGCGATCCAGTTGCTGCTGGCTCAGCCCGGCCAGTGGGAAGTCGCTTGGCGTAATGCCTTGCGCCTCa    <  5:66677/148‑1 (MQ=255)
                                                                                                                                                  gAGCAAGGTGTGCATCAGCAGCCCTTCCTGCATCGGCGACAACGGATAGATGTCTTCGATATCGCGCGCGGCCACCGGCAGGCGATCCAGTTGCTGCTGGCTCAGCCCGGCCAGTGGGAAGTCGCTTGGCGTAATGCCTTGCGCCTCa    <  5:161373/148‑1 (MQ=255)
                                                                                                                                                   aGCAAGGTGTGCATCAGCAGCCCTTCCTGCATCGGCGACAACGGATAGATGTCTTCGATATCGCGCGCGGCCACCGGCAGGCGATCCAGTTGCTGCTGGCTCAGCCCGGCCAGTGGGAAGTCGCTTGGCGTAATGCCTTGCGCCTCAgg  >  8:172171/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
CGCTCACCCAGGTCCCAGCAAAACGAGGTGCGCAGCACGTCATGGCGCGCAATGACCGCTTTCCAGGCTTCGCTGAACGCGCCGACATCAATGGCGTGGTCGACGCGGTAGCAATACTGCATCAGATAGATGCCCGAACCCGGTTCGAGCAAGGTGTGCATCAGCAGCCCTTCCTGCATCGGCGACAACGGATAGATGTCTTCGATATCGCGCGCGGCCACCGGCAGGCGATCCAGTTGCTGCTGGCTCAGCCCGGCCAGTGGGAAGTCGCTTGGCGTAATGCCTTGCGCCTCAGG  >  NC_002947/4782461‑4782756

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑