Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,824,272 G→C 6.9% S2306R (AGC→AGG)  pvdL ← non‑ribosomal peptide synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,824,2720GC 6.9% 174.9 / 4.7 58S2306R (AGC→AGG) pvdLnon‑ribosomal peptide synthetase
Reads supporting (aligned to +/- strand):  ref base G (26/28);  new base C (2/2);  total (28/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.92e-01

ACCGCCTGGCAGTGCATGGCGAACTCGCCATGGCTGACCACCACGCCCTTGGGTTTGCCGGTGGAGCCTGAGGTGTAGATCAGGTAAGCCTGGTGCTGCGGCAGGCTGAGGTTGTCCAGCGGTGCATCGCTGTAGGCCGCCAGGCTGGCGGCATCGTCTTCCAGGCTCCAGCTGGCCACGCCTTCAGGCAGCTCGCCCAGGGTGTGCAGCAGCGCACGCTGGCTGAGCAGCAAGCCGATGCGGCTGTCTTCGATCATGTAGCGCAGGCGGTCGAGCGGGTACTCGGGG  >  NC_002947/4824129‑4824416
                                                                                                                                               |                                                                                                                                                
aCCGCCTGGCAGTGCATGGCGAACTCGCCATGGCTGACCACCACGCCCTTGGGTTTGCCGGTGGAGCCTGAGGTGTAGATCACGTAAGACTCCTTATCCTTAAAAATCAGGTTGTCCAGCGGTGCATCGCTGTAGGCCGCCAGGCTggc                                                                                                                                             >  6:2527/1‑149 (MQ=21)
   gCCTGGCAGTGCATGGCGAACTCGCCATGGCTGACCACCACGCCCTTGGGTTTGCCGGTGGAGCCTGAGGTGTAGATCAGGTAAGCCTGGTGCTGCGGCAGGCTGAGGTTGTCCAGCGGTGCATCGCTGTAGGCCGCCAGGCTggcgg                                                                                                                                           >  1:48701/1‑148 (MQ=255)
         cAGTGCATGGCGAACTCGCCATGGCTGACCACCACGCCCTTGGGTTTGCCGGTGGAGCCTGAGGTGTAGATCAGGTAAGCCTGGTGCTGCGGCAGGCTGAGGTTGTCCAGCGGTGCATCGCTGTAGGCCGCCAGGCTGGCGGCATCGTc                                                                                                                                    >  3:47496/1‑149 (MQ=255)
         cAGTGCATGGCGAACTCGCCATGGCTGACCACCACGCCCTTGGGTTTGCCGGTGGAGCCTGAGGTGTAGATCAGGTAAGCCTGGTGCTGCGGCAGGCTGAGGTTGTCCAGCGGTGCATCGCTGTAGGCCGCCAGCCTggcggcctacag                                                                                                                                    <  7:108851/149‑7 (MQ=255)
              cATGGCGAACTCGCCATGGCTGACCACCACGCCCTTGGGTTTGCCGGTGGAGCCTGAGGTGTAGATCAGGTAAGCCTGGTGCTGCGGCAGGCTGAGGTTGTCCAGCGGTGCATCGCTGTAGGCCGCCAGGCTGGCGGCATCGTCTTCCa                                                                                                                               <  8:24272/149‑1 (MQ=255)
                tGGCGAACTCGCCATGGCTGACCACCACGCCCTTGGGTTTGCCGGTGGAGCCTGAGGTGTAGATCAGGTAAGCCTGGTGCTGCGGCAGGCTGAGGTTGTCCAGCGGTGCATCGCTGTAGGCCGCCAGGCTGGCGGCATCGTCTTCCAgg                                                                                                                             >  1:324617/1‑149 (MQ=255)
                     aaCTCGCCATGGCTGACCACCACGCCCTTGGGTTTGCCGGTGGAGCCTGATGTGTAGATCAGGTAAGCCTGGTGCTGCGGCAGGCTGAGGTTGTCCAGCGGTGCATCGCTGTAGGCCGCCAGGCTGGCGGCATCGTCTTCCAGGCTCCa                                                                                                                        <  1:117785/149‑1 (MQ=255)
                        tCGCCATGGCTGACCACCACGCCCTTGGGTTTGCCGGTGGAGCCTGAGGTGTAGATCAGGTAAGCCTGGTGCTGCGGCAGGCTGAGGTTGTCCAGCGGTGCATCGCTGTAGGCCGCCAGGCTGGCGGCATCGTCTTCCAgg                                                                                                                             <  8:16277/141‑1 (MQ=255)
                        tCGCCATGGCTGACCACCACGCCCTTGGGTTTGCCGGTGGAGCCTGAGGTGTAGATCAGGTAAGCCTGGTGCTGCGGCAGGCTGAGGTTGTCCAGCGGTGCATCGCTGTAGGCCGCCAGGCTGGCGGCATCGTCTTCCAgg                                                                                                                             >  7:16277/1‑141 (MQ=255)
                          gCCATGGCTGACCACCACGCCCTTGGGTTTGCCGGTGGAGCCTGAGGTGTAGATCAGGTAAGCCTGGTGCTGCGGCAGGCTGAGGTTGTCCAGCGGTGCATCGCTGTAGGCCGCCAGGCTGGCGGCATCGTCTTCCAGGCTCCAGCTgg                                                                                                                   >  7:80371/1‑149 (MQ=255)
                          gCCATGGCTGACCACCACGCCCTTGGGTTTGCCGGTGGAGCCTGAGGTGTAGATCAGGTAAGCCTGGTGCTGCGGCAGGCTGAGGTTGTCCAGCGGTGCATCGCTGTAGGCCGCCAGGCTGGCGGCATCGTCTTCCAGGCTCCAGCTg                                                                                                                    <  3:355313/148‑1 (MQ=255)
                           gcATTGCTGACCACCACGCCATTGGGTTTGCCGGTTGAGCCTGAGGTGTAGATCAGGTAAGCCTGGTGCTGCGGCAGGCTGAGGTTGTCCAGCGGTGCATCGCTGTAGGCCGCCAGGCTGGCGGCATCGTCTTCCAGGCTCCAGCTGGc                                                                                                                  <  8:237410/148‑1 (MQ=255)
                            cATGGCTGACCACCACGCCCTTGGGTTTGCCGGTGGAGCCTGAGGTGTAGATCAGGTAAGCCTGGTGCTGCGTCAGGCTGAGGTTGTCCAGCGGTGCATCGCTGTAGGCCGCCAGGCTGGCGGCATCGTCTTCCAGGCTCCAGCTGGcc                                                                                                                 >  8:86149/1‑149 (MQ=255)
                                gCTGACCACCACGCCCTTGGGTTTGCCGGTGGAGCCTGAGGTGTAGATCAGGTAAGCCTGGTGCTGCGGCAGGCTGAGGTTGTCCAGCGGTGCATCGCTGTAGGCCGCCAGGCTGGCGGCATCGTCTTCCAGGCTCCAGCTGGCCACGc                                                                                                             <  6:161037/149‑1 (MQ=255)
                                      caccacGCCCTTGGGTTTGCCGGTGGAGCCTGAGGTGTAGATCAGGTAAGCCTGGTGCTGCGGCAGGCTGAGGTTGTCCAGCGGTGCATCGCTGTAGGCCGCCAGGCTGGCGGCATCGTCTTCCAGGCTCCAGCTGGCCACGCCTTCAg                                                                                                       <  2:48701/149‑1 (MQ=255)
                                        ccacGCCCTTGGGTTTGCCGGTGGAGCCTGAGGTGTAGATCAGGTAAGCCTGGTGCTGCGGCAGGCTGAGGTTGTCCAGCGGTGCATCGCTGTAGGCCGCCAGGCTGGCGGCATCGTCTTCCAGGCTCCAGCTGGCCACGCCTTCAGGc                                                                                                     <  2:311778/149‑1 (MQ=255)
                                         cacGCCCTTGGGTTTGCCGGTGGAGCCTGAGGTGTAGATCAGGTAAGCCTGGTGCTGCGGCAGGCTGAGGTTGTCCAGCGGTGCATCGCTGTAGGCCGCCAGGCTGGCGGCATCGTCTTCCAGGCTCCAGCTgg                                                                                                                   >  5:71404/1‑134 (MQ=255)
                                         cacGCCCTTGGGTTTGCCGGTGGAGCCTGAGGTGTAGATCAGGTAAGCCTGGTGCTGCGGCAGGCTGAGGTTGTCCAGCGGTGCATCGCTGTAGGCCGACAGGCTGGCGGCATCGTCTTCCAGGCTCCAGCTgg                                                                                                                   <  6:71404/134‑1 (MQ=255)
                                            gCCCTTGGGTTTGCCGGTGGAGCCTGAGGTGTAGATCAGGTAAGCCTGGTGCTGCGGCAGGCTGAGGTTGTCCAGCGGTGCATCGCTGTAGGCCGCCAGGCTGGCGGCATCGTCTTCCAGGCTCCAGCTGGCCACGCCTTCAGGCAGCt                                                                                                 <  6:242678/149‑1 (MQ=255)
                                                ttGGGTTTGCCGGTGGAGCCTGAGGTGTAGATCAGGTAAGCCTAGTGCTGCGGCAGGCTGAGGTTGTCCAGCGGTGCATCGCTGTAGGCCGCCAGGCTGGCGGCATCGTCTTCCAGGCTCCAGCTGGCCACGCCTTCAGGCAGCTCGcc                                                                                             >  1:258025/1‑149 (MQ=255)
                                                  gggTTTGCCGGTGGAGCCTGAGGTGTAGATCAGGTAAGCCTGGTGCTGCGGCAGGCTGAGGTTGTCCAGCGGTGCATCGCTGTAGGCCGCCAGGCTGGCGGCATCGTCTTCCAGGCTCCAGCTGGCCACGCCTTCAGGCAGCTCGcc                                                                                             >  7:201307/1‑147 (MQ=255)
                                                          cGGTGGAGCCTGAGGTGTAGATCAGGTAAGCCTGGTGCTGCGGCAGGCTGAGGTTGTCCAGCGGTGCATCGCTGTAGGCCGCCAGGCTGGCGGCATCGTCTTCCAGGCTCCAGCTGGCCACGCCTTCAGGCAGCTc                                                                                                <  6:15223/136‑1 (MQ=255)
                                                          cGGTGGAGCCTGAGGTGTAGATCAGGTAAGCCTGGTGCTGCGGCAGGCTGAGGTTGTCCAGCGGTGCATCGCTGTAGGCCGCCAGGCTGGCGGCATCGTCTTCCAGGCTCCAGCTGGCCACGCCTTCAGGCAGCTc                                                                                                >  5:15223/1‑136 (MQ=255)
                                                           ggTGGAGCCTGAGGTGTAGATCAGGTAAGCCTGGTGCTGCGGCAGGCTGAGGTTGTCCAGCGGTGCATCGCTGTAGGCCGCCAGGCTGGCGGCATCGTCTTCCAGGCTCCAGCTGGCCACGCCTTCAGGCAGCTCGCCCAGGGTGTgca                                                                                  <  5:2527/149‑1 (MQ=255)
                                                                 ggatGAGGTGTAGATGATGTATGCCTGGTTGTTCGGCAGGCTGAGGTTGTACAGCGGTGCATCGATGTAGGACGCCAGGCTGGCGGCATCGTCTTCCAGGCTGCAGCTGTCCAAGCCTTCAGTCAGCTAGAACAGGGTGTgcaggatc                                                                             <  2:258025/145‑5 (MQ=255)
                                                                          gtAGATCAGGTAAGCCTGGTGCTGCGGCAGGCTGAGGTTGTCCAGCGGTGCATCGCTGTAGGCCGCCAGGCTGGCGGCATCGTCTTCCAGGCTCCAGCTGGCCACGCCTTCAGGCAGCTCGCCCAGGGTGTGCAGCAGCGCACgctggc                                                                   >  5:379108/1‑149 (MQ=255)
                                                                           tAGATCAGGTAAGCCTGGTGCTGCGGCAGGCTGAGGTTGTCCAGCGGTGCATCGCTGTAGGCCGCCAGGCTGGCGGCATCGTCTTCCAGGCTCCAGCTGGCCACGCCTTCAGGCAGCTCGCCCAGGGTGTGCAGCAGCGCACgctggc                                                                   <  7:86149/148‑1 (MQ=255)
                                                                             gATCAGGTAAGCCTGGTGCTGCGGCAGGCTGAGGTTGTCCAGCGGTGCATCGCTGTAGGCCGCCAGGCTGGCGGCATCGTCTTCCAGGCTCCAGCTGGCCACGCCTTCAGGCAGCTCGCCCAGGGTGTGCAGCAGCGCACgctggctg                                                                 <  2:324617/148‑1 (MQ=255)
                                                                             gATCAGGTAAGCCTGGTGCTGCGGCAGGCTGAGGTTGTCCAGCGGTGCATCGCTGTAGGCCGCCAGGCTGGCGGCATCGTCTTCCAGGCTCCAGCTGGCCACGCCTTCAGGCAGCTCGCCCAGGGTGTGCAGCAGCGCACgctggct                                                                  >  4:303686/1‑147 (MQ=255)
                                                                             gATCAGGTAAGCCTGGTGCTGCGGCAGGCTGAGGTTGTCCAGCGGTGCATCGCTGTAGGCCGCCAGGCTGGCGGCATCGTCTTCCAGGCTCCAGCTGGCCACGCCTTCAGGCAGCTCGCCCAGGGTGTGCAGCAGCGCACGCTGGCTGa                                                                <  4:47496/149‑1 (MQ=255)
                                                                                     aaGCCTGGTGCTGCGGCAGGCTGAGGTTGTCCAGCGGTGCATCGCTGTAGGCCGCCAGGCTGGCGGCATCGTCTTCCAGGCTCCAGCTGGCCACGCCTTCAGGCAGCTCGCCCAGGGTGTGCAGCAGCGCACGCTGGCTGAGCAGCAAg                                                        >  4:30563/1‑149 (MQ=255)
                                                                                      aGCCTGGTGCTGCGGCAGGCTGAGGTTGTCCAGCGGTGCATCGCTGTAGGCCGCCAGGCTGGCGGCATCGTCTTCCAGGCTCCAGCTGGCCACGCCTTCAGGCAGCTCGCCCAGGGTGTGCAACAGCGCACGCTGGCTGAGCAGCAAGc                                                       <  8:80371/149‑1 (MQ=255)
                                                                                        ccTGGTGCTGCGGCAGGCTGAGGTTGTCCAGCGGTGCATCGCTGTAGGCCGCCAGGCTGGCGGCATCGTCTTCCAGGCTCCAGCTGGCCACGCCTTCAGGCAGCTCGCCCAGGGTGTGCAGCAGCGCACGCTGGCTGAGCAGCAAGcc                                                      >  5:167891/1‑148 (MQ=255)
                                                                                        ccTGGTGCTGCGGCAGGCTGAGGTTGTCCAGCGGTGCATCGCTGTAGGCCGCCAGGCTGGCGGCATCGTCTTCCAGGCTCCAGCTGG‑CACGCCTTCAGGCAGCTCAGCCAGCGTGCGCTGCTGCACACCCTGGGCGagctgcct                                                         <  4:64078/144‑3 (MQ=25)
                                                                                        ccTGGTGCTGCGGCAGGCTGAGGTTGTCCAGCGGTGCATCGCTGTAGGCCGCCAGGCTGGCGGCATCGTCTTCCAGGCTCCAGCTGG‑CACGCCTTCAGGCAGCTCAGCCAGCGTGCGCTGCTGCACACCCTGGGCGagctgcct                                                         >  3:64078/1‑142 (MQ=25)
                                                                                         cTGGTGCTGCGGCAGGCTGAGGTTGTCCAGCGGTGCATCGCTGTAGGCCGCCAGGCTGGCGGCATCGTCTTCCAGGCTCCAGCTGGCCACGCCTTCAGGCAGCTCGCCCAGGGTGTGCAGCAGCGCACGCTGGCTGAGCAGCAAGCCGa                                                    >  2:334086/1‑149 (MQ=255)
                                                                                            gTGCTGCGGCAGGCTGAGGTTGTCCAGCGGTGCATCGCTGTAGGCCGCCAGGCTGGCGGCATCGTCTTCCAGGCTCCAGCTGGCCACGCCTTCAGGCAGCTCGCCCAGGGTGTGCAGCAGCGCACGCTGGCTGAGCAGCAAGCCGATGc                                                 <  1:334086/149‑1 (MQ=255)
                                                                                              gctgcGGCAGGCTGAGGTTGTCCAGCGGTGCATCGCTGTAGGCCGCCAGGCTGGCGGCATCGTCTTCCAGGCTCCAGCTGGCCACGCCTTCAGGCAGCTCGCCCAGGGTGTGCAGCAGCGCACGCTGGCTGAGCAGCAAGCCGATGCgg                                               <  8:201307/149‑1 (MQ=255)
                                                                                                   ggCAGGCTGAGGTTGTCCAGCGGTGCATCGCTGTAGGCCGCCAGGCTGGCGGCATCGTCTTCCAGGCTCCAGCTGGCCACGCCTTCAGGCAGCTCGCCCAGGGTGTGCAGCAGCGCACGCTGGCTGAGCAGCAAGCCGATGCGGCTGTc                                          >  5:305967/1‑149 (MQ=255)
                                                                                                        gCTGAGGTTGTCCAGCGGTGCATCGCTGTAGGCCGCCAGGCTGGCGGCATCGTCTTCCAGGCTCCAGCTGGCCACGCCTTCAGGCAGCTCGCCCAGGGTGTGCAGCAGCGCACGCTGGCTGAGCAGCAAGCCGATGCGGCTGTCTTCGa                                     <  1:163222/149‑1 (MQ=255)
                                                                                                        gCTGAGGTTGTCCAGCGGTGCATCGCTGTAGGCCGCCAGGCTGGCGGCATCGTCTTCCAGGCTCCAGCTGGCCACGCCTTCAGGCAGCTCGCCCAGGGTGTGCAGCAGCGCACGCTGGCTGAGCAGCAAGCCGATGCGGCTGTCTTCGa                                     >  6:190158/1‑149 (MQ=255)
                                                                                                                              tCGCTGTAGGCCGCCAGGCTGGCGGCATCGTCTTCCAGGCTCCAGCTGGCCACGCCTTCAGGCAGCTCGCCCAGGGTGTGCAGCAGCGCACGCTGGCTGAGCAGCAAGCCGATg                                                  <  1:217505/114‑1 (MQ=255)
                                                                                                                              tCGCTGTAGGCCGCCAGGCTGGCGGCATCGTCTTCCAGGCTCCAGCTGGCCACGCCTTCAGGCAGCTCGCCCAGGGTGTGCAGCAGCGCACGCTGGCTGAGCAGCAAGCCGATg                                                  >  2:217505/1‑114 (MQ=255)
                                                                                                                              tCGCTGTAGGCCGCCAGGCTGGCGGCATCGTCTTCCAGGCTCCAGCTGGCCACGCCTTCAGGCAGCTCGCCCAGGGTGTGCAGCAGCGCACGCTGGCTGAGCAGCAAGCCGATGCGGCTGTCTTCGATCATGTAGCGCAGGCGGTCGAg               >  3:93103/1‑149 (MQ=255)
                                                                                                                                gCTGTAGGCCGCCAGGCTGGCGGCATCGTCTTCCAGGCTCCAGCTGGCCACGCCTTCAGGCAGCTCGCCCAGGGTGTGCAGCAGCGCACGCTGGCTGAGCAGCAAGCCGATGCGGCTGTCTTCGATCATGTAGCGCAGGCGGTCGAGc              <  3:30563/148‑1 (MQ=255)
                                                                                                                                 cTGTAGGCCGCCAGGCTGGCGGCATCGTCTTCCAGGCTCCAGCTGGCCACGCCTTCAGGCAGCTCGCCCAGGGTGTGCAGCAGCGCACGCTGGCTGAGCAGCAAGCCGATGCGGCTGTCTTCGATCATGTAGCGCAGGCGGTCGAGCgg            >  7:254917/1‑149 (MQ=255)
                                                                                                                                 cTGTAGGCCGCCAGGCTGGCGGCATCGTCTTCCAGGCTCCAGCTGGCCACGCCTTCAGGCAGCTCGCCCAGGGTGTGCAGCAGCGCACGCTGGCTGAGCAGCAAGCCGATGCGGCTGTCTTCGATCATGTAGCGCAGGCGGTCGAGCgg            <  6:379108/149‑1 (MQ=255)
                                                                                                                                  tGTAGGCCGCCAGGCTGGCGGCATCGTCTTCCAGGCTCCAGCTGGCCACGCCTTCAGGCAGCTCGCCCAGGGTGTGCAGCAGCGCACGCTGGCTGAGCAGCAAGCGGATGCGGCTGTCTTCGATCATGTAGCGCAGGCGGTCGAGCggg           >  3:201703/1‑149 (MQ=255)
                                                                                                                                  tGTAGGCCGCCAGGCTGGCGGCATCGTCTTCCAGGCTCCAGCTGGCCACGCCTTCAGGCAGCTCGCCCAGGGTGTGCAGCAGCGCACGCTGGCTGAGCAGCAAGCCGATGCGGCTGTCTTCGATCATGTAGCGCAGGCGGTCGAGCggg           >  2:87355/1‑149 (MQ=255)
                                                                                                                                   cgatgccgccAGCCTGGCGGCATCGTCTTCCAGGCTCCAGCTGGCCACGCCTTCAGGCAGCTCGCCCAGGGTGTGCAGCAGCGCACGCTGGCTGAGCAGCAAGCCGATGCGGCTGTCTTCGATCATGTAGCGCAGGc                      <  3:338081/133‑1 (MQ=255)
                                                                                                                                   cgatgccgccAGCCTGGCGGCATCGTCTTCCAGGCTCCAGCTGGCCACGCCTTCAGGCAGCTCGCCCAGGGTGTGCAGCAGCGCACGCTGGCTGAGCAGCAAGCCGATGCGGCTGTCTTCGATCATGTAGCGCAGGc                      >  4:338081/5‑137 (MQ=255)
                                                                                                                                    tAGGCCGCCAGGCTGGCGGCATCGTCTTCCAGGCTCCAGCTGGCCACGCCTTCAGGCAGCTCGCCCAGGGTGTGCAGCAGCGCACGCTGGCTGAGCAGCAAGCCGATGCGGCTGTCTTCGATCATGTAGCGCAGGCGGTCGAGCggg           <  5:190158/147‑1 (MQ=255)
                                                                                                                                    tAGGCCGCCAGGCTGGCGGCATCGTCTTCCAGGCTCCAGCTGGCCACGCCTTCAGGCAGCTCGCCCAGGGTGTGCAGCAGCGCACGCTGGCTGAGCAGCAAGCCGATGCGGCTGTCTTCGATCATGTAGCGCAGGCGGTCGAGCGGGTa         >  5:84427/1‑149 (MQ=255)
                                                                                                                                     atgccgccAGCCTGGCGGCATCGTCTTCCAGGCTCCAGCTGGCCACGCCTTCAGGCAGCTCGCCCAGGGTGTGCAGCAGCGCACGCTGGCTGAGCAGCAAGCCGATGCGGCTGTCTTCGATCATGTAGCGCAGGCGGTCGAGCGGGTAc        >  7:172233/3‑149 (MQ=255)
                                                                                                                                      ggCCGCCAGGCTGGCGGCATCGTCTTCCAGGCTCCAGCTGGCCACGCCTTCAGGCAGCTCGCCCAGGGTGTGCAGCAGCGCACGCTGGCTGAGCAGCAAGCCGATGCGGCTGTCTTCGATCATGTAGCGCAGGCGGTCGAGCGGGTAc        <  6:305967/148‑1 (MQ=255)
                                                                                                                                         cgccAGGCTGGCGGCATCGTCTTCCAGGCTCCAGCTGGCCACGCCTTCAGGCAGCTCGCCCAGGGTGTGCAGCAGCGCACGCTGGCTGAGCAGCAAGCCGATGCGGCTGTCTTCGATCATGTAGCGCAGGCGGTCGAGCgg            <  2:194238/141‑1 (MQ=255)
                                                                                                                                         cgccAGGCTGGCGGCATCGTCTTCCAGGCTCCAGCTGGCCACGCCTTCAGGCAGCTCGCCCAGGGTGTGCAGCAGCGCACGCTGGCTGAGCAGCAAGCCGATGCGGCTGTCTTCGATCATGTAGCGCAGGCGGTCGAGCgg            >  1:194238/1‑141 (MQ=255)
                                                                                                                                           ccAGGCTGGCGGCATCGTCTTCCAGGCTCCAGCTGGCCACGCCTTCAGGCAGCTCGCCCAGGGTGTGCAGCAGCGCACGCTGGCTGAGCAGCAAGCCGATGCGGCTGTCTTCGATCATGTAGCGCAGGCGGTCGAGCGGGTACTCgggg  <  3:303686/149‑1 (MQ=255)
                                                                                                                                               |                                                                                                                                                
ACCGCCTGGCAGTGCATGGCGAACTCGCCATGGCTGACCACCACGCCCTTGGGTTTGCCGGTGGAGCCTGAGGTGTAGATCAGGTAAGCCTGGTGCTGCGGCAGGCTGAGGTTGTCCAGCGGTGCATCGCTGTAGGCCGCCAGGCTGGCGGCATCGTCTTCCAGGCTCCAGCTGGCCACGCCTTCAGGCAGCTCGCCCAGGGTGTGCAGCAGCGCACGCTGGCTGAGCAGCAAGCCGATGCGGCTGTCTTCGATCATGTAGCGCAGGCGGTCGAGCGGGTACTCGGGG  >  NC_002947/4824129‑4824416

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑