Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 387,792 C→T 8.4% S311F (TCC→TTC) ‡ glyA‑I → serine hydroxymethyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947387,7920CT 8.4% 171.4 / 3.5 59S311F (TCC→TTC) ‡glyA‑Iserine hydroxymethyltransferase
Reads supporting (aligned to +/- strand):  ref base C (28/26);  new base T (3/2);  total (31/28)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.86e-01

CCCGCTGATGCACGTGATCGCTGCCAAGGCAGTGTGCTTCAAGGAAGCGCTGGAGCCTGGATTCAAGGCCTACCAGCAGCAAGTGATCGAAAACGCCCAGGCCATGGCCCAGGTGTTCATCGACCGTGGCTACGATGTGGTTTCCGGTGGCACCGACAACCACCTGTTCCTGGTCAGCCTGATCCGTCAGGGCCTGACTGGCAAAGACGCCGACGCCGCCCTGGGTCGTGCGCATATCACCGTCAACAAGAACGCCGTGCCGAACGACCCGCAGTCGCCGTTCGT  >  NC_002947/387649‑387933
                                                                                                                                               |                                                                                                                                             
cccGCTGATGCACGTGATCGCTGCCAAGGCAGTGTGCTTCAAGGAAGCGCTGGAGCCTGGATTCAAGGCCTACCAGCAGCAAGTGATCGAAAACGCCCAGGCCATGGCCCAGGTGTTCATCGACCGTGGCTACGATGTGGTTTCCGGTg                                                                                                                                          >  6:286175/1‑149 (MQ=255)
 ccGCTGATGCACGTGATCGCTGCCAAGGCAGTGTGCTTCAAGGAAGCGCTGGAGCCTGGATTCAAGGCCTACCAGCAGCAAGTGATCGAAAACGCCCAGGCCATGGCCCAGGTGTTCATCGACCGTGGCTACGATGTGGTTTCCGGTgg                                                                                                                                         >  4:245486/1‑149 (MQ=255)
    cTGATGCACGTGATCGCTGCCAAGGCAGTGTGCTTCAAGGAAGCGCTGGAGCCTGGATTCAAGGCCTACCAGCAGCAAGTGATCGAAAACGCCCAGGCCATGGCCCAGGTGTTCATCGACCGTGGCTACGATGTGGTTTCCGGTGGCAc                                                                                                                                      <  2:234958/149‑1 (MQ=255)
     tGATGCACGTGATCGCTGCCAAGGCAGTGTGCTTCAAGGAAGCGCTGGAGCCTGGATTCAAGGCCTACCAGCAGCAAGTCATCGAAAACGCCCAGGCCATGGCCCAGGAGTTCATCGACCGTGGCTACGATGTGGTTTCCGGTGGCAcc                                                                                                                                     >  2:123230/1‑149 (MQ=255)
        tGCACGTGATCGCTGCCAAGGCAGTGTGCTTCAAGGAAGCGCTGGAGCCTTGATTCAAGGCCTACCAGCAGCAAGTGATCGAAAACGCCCAGGCCATGGCCCAGGTGTTCATCGACCGTGGCTACGATGTGGTTTCCGGTGGCACCGAc                                                                                                                                  <  1:243404/149‑1 (MQ=255)
            cGTGATCGCTGCCAAGGCAGTGTGCTTCAAGGAAGCGCTGGAGCCTGGATTCAAGGCCTACCAGCAGCAAGTGATCGAAAACGCCCAGGCCATGGCCCAGGTGTTCATCGACCGTGGCTACGATGTGGTTTCCGGTGGCACCGACAac                                                                                                                               >  4:191705/1‑148 (MQ=255)
                 tCGCTGCCAAGGCAGTGTGCTTCAAGGAAGCGCTGGAGCCTGGATTCAAGGCCTACCAGCAGCAAGTGATCGAAAACGCCCAGGCCATGGCCCAGGTGTTCATCGACCGTGGCTACGATGTGGTTGTCGGTGCCACCGGAAaccacatc                                                                                                                         <  1:88765/149‑4 (MQ=255)
                    cTGCCAAGGCAGTGTGCTTCAAGGAAGCGCTGGAGCCTGGATTCAAGGCCTACCAGCAGCAAGTGATCGAAAACGCCCAGGCCATGGCCCAGGTGTTCATCGACCGTGGCTACGATGTGGTTTCCGGTGGCACCGACAACCACCTGTTc                                                                                                                      <  3:245486/149‑1 (MQ=255)
                       ccAAGGCAGTGTGCTTCAAGGAAGCGCTGGAGCCTGGATTCAAGGCCTACCAGCAGCAAGTGATCGAAAACGCCCAGGCCATGGCCCAGGTGTTCATCGACCGTGGCTACGATGTGGTTTCCGGTGGCACCGACAACCACCTGTTCCTg                                                                                                                   <  3:162802/149‑1 (MQ=255)
                        cAAGGCAGTGTGCTTCAAGGAAGCGCTGGAGCCTGGATTCAAGGCCTACCAGCAGCAAGTGATCGAAAACGCCCAGGCCATGGCCCAGGTGTTCATCGACCGTGGCTACGATGTGGTTTCCGGTGGCACCGACAACCACCTGTTCCTg                                                                                                                   <  1:3805/148‑1 (MQ=255)
                        cAAGGCAGTGTGCTTCAAGGAAGCGCTGGAGCCTGGATTCAAGGCCTACCAGCAGCAAGTGATCGAAAACGCCCAGGCCATGGCCCAGGTGTTCATCGACCGTGGCTACGATGTGGTTTCCGGTGGCACCGACAACCACCTGTTCCTg                                                                                                                   >  2:3805/1‑148 (MQ=255)
                           ggCAGTGTGCTTCAAGGAAGCGCTGGAGCCTGGATTCAAGGCCTACCAGCAGCAAGTGATCGAAAACGCCCAGGCCATGGCCCAGGTGTTCATCGACCGTGGCTACGATGTGGTTTCCGGTGGCACCGAc                                                                                                                                  <  4:173080/130‑1 (MQ=255)
                           ggCAGTGTGCTTCAAGGAAGCGCTGGAGCCTGGATTCAAGGCCTACCAGCAGCAAGTGATCGAAAACGCCCAGGCCATGGCCCAGGTGTTCATCGACCGTGGCTACGATGTGGTTTCCGGTGGCACCGAc                                                                                                                                  >  3:178757/1‑130 (MQ=255)
                           ggCAGTGTGCTTCAAGGAAGCGCTGGAGCCTGGATTCAAGGCCTACCAGCAGCAAGTGATCGAAAACGCCCAGGCCATGGCCCAGGTGTTCATCGACCGTGGCTACGATGTGGTTTCCGGTGGCACCGAc                                                                                                                                  >  3:173080/1‑130 (MQ=255)
                           ggCAGTGTGCTTCAAGGAAGCGCTGGAGCCTGGATTCAAGGCCTACCAGCAGCAAGTGATCGAAAACGCCCAGGCCATGGCCCAGGTGTTCATCGACCGTGGCTACGATGTGGTTTCCGGTGGCACCGAc                                                                                                                                  <  4:178757/130‑1 (MQ=255)
                           ggCAGTGTGCTTCAAGGAAGCGCTGGAGCCTGGATTCAAGGCCTACCAGCAGCAAGTGATCGAAAACGCCCAGGCCATGGCCCAGGTGTTCATCGACCGTGGCTACGATGTGGTTTCCGGTGGCACCGACAACCACCTGTTCCTGGTCa                                                                                                               <  6:312384/149‑1 (MQ=255)
                                     ttCAAGGAAGCGCTGGAGCCTGGATTCAAGGCCTACCAGCAGCAAGTGATCGAAAACGCCCAGGCCATGGCCCAGGTGTTCATCGACCGTGGCTACGATGTGGTTGTCGGTGCCACCGGAAaccacatc                                                                                                                         >  3:247067/1‑126 (MQ=255)
                                     ttCAAGGAAGCGCTGGAGCCTGGATTCAAGGCCTACCAGCAGCAAGTGATCGAAAACGCCCAGGCCATGGCCCAGGTGTTCATCGACCGTGGCTACGATGTGGTTGTCGGTGCCACCGGAAaccacatc                                                                                                                         <  4:247067/129‑4 (MQ=255)
                                       cAAGGAAGCGCTGGAGCCTGGATTCAAGGCCTACCAGCAGCAAGTGATCGAAAACGCCCAGGCCATGGCCCAGGTGTTCATCGACCGTGGCTACGATGTGGTTTCCGGTGGCACCGACAACCACCTGTTCCTGGTCAGCCTGATCCGt                                                                                                    >  6:123136/1‑148 (MQ=255)
                                        aaGGAAGCGCTGGAGCCTGGATTCAAGGCCTACCAGCAGCAAGTGATCGAAAACGCCCAGGCCATGGCCCAGGTGTTCATCGACCGTGGCTACGATGTGGTTTCCGGTGGCACCGACAACCACCTGTTCCTGGTCAGCCTGATCCGt                                                                                                    >  6:129923/1‑147 (MQ=255)
                                          ggAAGCGCTGGAGCCTGGATTCAAGGCCTACCAGCAGCAAGTGATCGAAAACGCCCAGGCCATGGCCCAGGTGTTCATCGACCGTGGCTACGATGTGGTTTCCGGTGGCACCGACAACCACCTGTTCCTGGTCAGCCTGATCCGTCAgg                                                                                                >  7:195867/1‑149 (MQ=255)
                                           gAAGCGCTGGAGCCTGGATTCAAGGCCTACCAGCAGCAAGTGATCGAAAACGCCCAGGCCATGGCCCAGGTGTTCATCGACCGTGGCTACGATGTGGTTTCCGGTGGCACCGACAACCACCTGTTCCTGGTCAGCCTGATCCGTCAggg                                                                                               <  5:286175/149‑1 (MQ=255)
                                           gAAGCGCTGGAGCCTGGATTCAAGGCCTACCAGCAGCAAGTGATCGAAAACGCCCAGGCCATGGCCCAGGTGTTCATCGACCGTGGCTACGATGTGGTTTCCGGTGGCACCGACAACCACCTGTTCCTGGTCAGCCTGATCCGTCAggg                                                                                               >  4:295062/1‑149 (MQ=255)
                                           gAAGCGCTGGAGCCTGGATTCAAGGCCTACCAGCAGCAAGTGATCAAAAACGCCCAGGCCATGGCCCAGGTGTTCATCGACCGTGGCTACGATGTGGTTTCCGGTGGCACCGACAACCACCTGTTCCTGGTCAGCCTGATCCGTCAggg                                                                                               <  8:182250/149‑1 (MQ=255)
                                              gcgcTGGAGCCTGGATTCAAGGCCTACCAGCAGCAAGTGATCGAAAACGCCCAGGCCATGGCCCAGGTGTTCATCGACCGTGGCTACGATGTGGTTTCCGGTGGCACCGACAACCACCTGTTCCTGGTCAGCCTGATCCGTCAGGGCCt                                                                                            >  2:96428/1‑149 (MQ=255)
                                                gcTGGAGCCTGGATTCAAGGCCTACCAGCAGCAAGTGATCGAAAACGCCCAGGCCATGGCCCAGGTGTTCATCGACCGTGGCTACGATGTGGTTTCCGGTGGCACCGACAACCACCTGTTCCt                                                                                                                    >  3:181719/1‑123 (MQ=255)
                                                gcTGGAGCCTGGATTCAAGGCCTACCAGCAGCAAGTGATCGAAAACGCCCAGGCCATGGCCCAGGTGTTCATCGACCGTGGCTACGATGTGGTTTCCGGTGGCACCGACAACCACCTGTTCCt                                                                                                                    <  4:181719/123‑1 (MQ=255)
                                                        cTGGATTCAAGGCCTACCAGCAGCAAGTGATCGAAAACGCCCAGGCCATGGCCCAGGTGTTCATCGACCGTGGCTACGATGTGGTTTCCGGTGGCACCGACAACCACCTGTTCCTGGTCAGCCTGATCCGTCAGGGCCTGACTGGCaaa                                                                                  <  3:191705/149‑1 (MQ=255)
                                                        cTGGATTCAAGGCCTACCAGCAGCAAGTGATCGAAAACGCCCAGGCCATGGCCCAGGTGTTCATCGACCGTGGCTACGATGTGGTTTCCGGTGGCACCGACAACCACCTGTTCCTGGTCAGCCTGATCCGTCAGGGCCTGACTGGCaaa                                                                                  <  3:365995/149‑1 (MQ=255)
                                                          ggATTCAAGGCCTACCAGCAGCAAGTGATCGAAAACGCCCAGGCCATGGCCCAGGTGTTCATCGACCGTGGCTACGATGTGGTTTCCGGTGGCACCGACAACCACCTGTTCCTGGTCAGCCTGATCCGTCAGGGCCTGACTGGCAAAga                                                                                >  5:211260/1‑149 (MQ=255)
                                                           gATTCAAGGCCTACCAGCAGCAAGTGATCGAAAACGCCCAGGCCATGGCCCAGGTGTTCATCGACCGTGGCTACGATGTGGTTTCCGGTGGCACCGACAACCACCTGTTCCTGGTCAGCCTGATCCGTCAGGGCCTGACTGGCAAAg                                                                                 <  8:195867/147‑1 (MQ=255)
                                                                aaGGCCTACCAGCAGCAAGTGATCGAAAACGCCCAGGCCATGGCCCAGGTGTTCATCGACCGTGGCTACGATGTGGTTTCCGGTGGCACCGACAACCACCTGTTCCTGGTCAGCCTGATCCGTCAGGGCCTGACTGGCAAAgacgccga                                                                          >  1:75103/1‑149 (MQ=255)
                                                                          agcagcAAGTGATCGAAAACGCCCAGGCCATGGCCCAGGTGTTCATCGACCGTGGCTACGATGTGGTTTCCGGTGGCACCGACAACCACCTGTTCCTGGTCAGCCTGATCCGTCAGGGCCTGACTGGCAAAGACGCCGACGCCGCCCTg                                                                <  5:123136/149‑1 (MQ=255)
                                                                                  gTGATCGAAAACGCCCAGGCCATGGCCCAGGTGTTCATCGACCGTGGCTTTGATGTGGTTTCCGGTGGCACCGACAACCACCTGTTCCTGGTCAGCCTGATCCGTCAGGGCCTGACTGGCAAAGACGCCGACGCCGCCCTGGGTCGTgc                                                        <  1:123230/149‑1 (MQ=255)
                                                                                  gTGATCGAAAACGCCCAGGCCATGGCCCAGGTGTTCATCGACCGTGGCTACGATGTGGTTTCCGGTGGCACCGACAACCACCTGTTCCTGGTCAGCCTGATCCGTCAGGGCCTGACTGGCAAAGACGCCGACGCCGCCCTGGGTCGTgc                                                        <  2:75103/149‑1 (MQ=255)
                                                                                  gTGATCGAAAACGCCCAGGCCATGGCCCAGGTGTTCATCGACCGTGGCTACGATGTGGTTTCCGGTGGCACCGACAACCACCTGTTCCTGGTCAGCCTGATCCGTCAGGGCCTGACTGGCAAAGACGCCGACGCCGCCCTGGGTCGTgc                                                        <  5:129923/149‑1 (MQ=255)
                                                                                    gATCGAAAACGCCCAGGCCATGGCCCAGGTGTTCATCGACCGTGGCTACGATGTGGTTTCCGGTGGCACCGACAACCACCTGTTCCTGGTCAGCCTGATCCGTCAGGGCCTGACTGGCAAAgacgcc                                                                            >  5:199059/1‑127 (MQ=255)
                                                                                    gATCGAAAACGCCCAGGCCATGGCCCAGGTGTTCATCGACCGTGGCTACGATGTGGTTTCCGGTGGCACCGACAACCACCTGTTCCTGGTCAGCCTGATCCGGCAGGGCCTGACTGGCAAAgacgcc                                                                            <  6:199059/127‑1 (MQ=255)
                                                                                     aTCGAAAACGCCCAGGCCATGGCCCAGGTGTTCATCGACCGTGGCTACGATGTGGTTTCCgg                                                                                                                                            >  6:301589/1‑62 (MQ=255)
                                                                                     aTCGAAAACGCCCAGGCCATGGCCCAGGTGTTCATCGACCGTGGCTACGATGTGGTTTCCgg                                                                                                                                            <  5:301589/62‑1 (MQ=255)
                                                                                     aTCGAAAACGCCCAGGCCATGGCCCAGGTGTTCATCGACCGTGGCTACGATGTGGTTTCCGGTGGCACCGACAACCACCTGTTCCTGGTCAGCCTGATCCGTCAGGGCCTGACTGGCAAAGACGCCGACGCCGCCCTGGGTCGTgcgc                                                      <  6:211260/148‑1 (MQ=255)
                                                                                     aTCGAAAACGCCCAGGCCATGGCCCAGGTGTTCATCGACCGTGGCTACGATGTGGTTTCCGGTGGCACCGACAACCACCTGTTCCTGGTCAGCCTGATCCGTCAGGGCCTGACTGGCAAAGACGCCGACGCCGCCCTGGGTCGTGCGCa                                                     >  2:322585/1‑149 (MQ=255)
                                                                                            aCGCCCAGGCCATGGCCCAGGTGTTCATCGACCGTGGCTACGATGTGGTTTCCGGTGGCACCGACAACCACCTGTTCCTGGTCAGCCTGATCCGTCAGGGCCTGACTGGTAAAGACGCCGACGCCGCCCTGGGTCGTGCGCATATCAcc                                              <  3:295062/149‑1 (MQ=255)
                                                                                              gCCCAGGCCATGGCCCAGGTGTTCATCGACCGTGGCTACGATGTGGTTTCCGGTGGCACCGACAACCACCTGTTCCTGGTCAGCCTGATCCGTCAGGGCCTGACTGGCAAAGACGCCGACGCCGCCCTGGGTCGTGCGCATATCACCGt                                            >  7:52011/1‑149 (MQ=255)
                                                                                                ccAGGCCATGGCCCAGGTGTTCATCGACCGTGGCTACGATGTGGTTTCCGGTGGCACCGACAACCACCTGTTCCTGGTCAGCCTGATCCGTCAGGGCCTGACTGGCAAAGACGCCGACGCCGCCCTGGGTCGTGCGCATATCACCGTc                                           >  1:203499/1‑148 (MQ=255)
                                                                                                  aGGCCATGGCCCAGGTGTTCATCGACCGTGGCTACGATGTGGTTTCCGGTGGCACCGACAACCACCTGTTCCTGGTCAGCCTGATCCGTCAGGGCCTGACTGGCAAAGACGCCGACGCCGCCCTGGGTCGTGCGCATATCACCGTcaac                                        <  8:29172/149‑1 (MQ=255)
                                                                                                      cATGGCCCAGGTGTTCATCGACCGTGGCTACGATGTGGTTTCCGGTGGCACCGACAACCACCTGTTCCTGGTCAGCCTGATCCGTCAGGGCCTGACTGGCAAAGACGCCGACGCCGCCCTGGGTCGTGCGCATATCACCGTCAACAAg                                     >  8:124489/1‑148 (MQ=255)
                                                                                                                   ttCATCGACCGTGGCTACGATGTGGTTTCCGGTGGCACCGACAACCACCTGTTCCTGGTCAGCCTGATCCGTCAGGGCCTGACTGGCAAAGACGCCGACGCCGCCCTGGGTCGTGCGCATATCACCGTCAACAAGAACGCCGTGCCGaa                       >  2:23149/1‑149 (MQ=255)
                                                                                                                    tattCGACCGTGGCTAGTTTGGGGTTTCGGGTGGCACGGACAACCACCTGTTCCTGTTCTGCTTGATCCGTCAGGGCCTGACTGGAAAAGAGGCCGACGCCGCCCTGGGTCGTGCGCATATCACCGTCAACAAGAACGCCGTGCCGaa                       <  1:96428/145‑1 (MQ=255)
                                                                                                                      aTCGACCGTGGCTACGATGTGGTTTCCGGTGGCACCGACAACCACCTGTTCCTGGTCAGCCTGATCCGTCAGGGCCTGACTGGCAAAGACGCCGACGCCGCCCTGGGTCGTGCGCATATCACCGTCAACAAGAACGCCGTGCCGAACGa                    >  8:174443/1‑149 (MQ=255)
                                                                                                                      aTCGACCGTGGCTACGATGTGGTTTCCGGTGGCACCGACAACCACCTGTTCCTGGTCAGCCTGATCCGTCAGGGCCTGACTGGCAAAGACGCCGACGCCGCCCTGGGTCGTGCGCATATCACCGTCAACAAGAACGCCGTGCCGAACGa                    >  8:211291/1‑149 (MQ=255)
                                                                                                                         gACCGTGGCTACGATGTGGTTTCCGGTGGCACCGACAACCACCTGTTCCTGGTCAGCCTGATCCGTCAGGGCCTGACTGGCAAAGACGCCGACGCCGCCCTGGGTCGTGCGCATATCACCGTCAACAAGAACGCCGTGCCGAACGAccc                 >  2:151044/1‑149 (MQ=255)
                                                                                                                                  tACGATGTGGTTTCCGGTGGCACCGACAACCACCTGTTCCTGGTCAGCCTGATCCGTCAGGGCCTGACTGGCAAAGACGCCGACGCCGCCCTGGGTCGTGCGCATATCACCGTCAACAAGAACGCCGTGCCGAACGACCCGCAGTCGcc        >  4:130633/1‑149 (MQ=255)
                                                                                                                                    cGATGTGGTTTCCGGTGGCACCGACAACCACCTGTTCCTGGTCAGCCTGATCCGTCAGGGCCTGACTGGCAAAGACGCCGACGCCGCCCTGGGTCGTGCGCATATCACCGTCAACAAGAACGCCGTGCCGAACGACCCGCAGTCGCCGt      <  1:144974/149‑1 (MQ=255)
                                                                                                                                      aTGTGGTTTCCGGTGGCACCGACAACCACCTGTTCCTGGTCAGCCTGATCCGTCAGGGCCTGACTGGCAAAGACGCCGACGCCGCCCTGGGTCGTGCGCATATCACCGTCAACAAGAACGCCGTGCCGAACGACCCGCAGTCGCCGTTc    <  4:361930/149‑1 (MQ=255)
                                                                                                                                       ggtgGTTGTCGGTGCCACCGGAAACCACCTGTTCCTGGTCAGCCTGATCCGTCAGGGCCTGACTGGCAAAGACGCCGACGCCGCCCTGGGTCGTGCGCATATCACCGTCAACAAGAACGCCGTGCCGAACGACCCGCAGTCGCCGTTCg   >  6:50391/2‑149 (MQ=255)
                                                                                                                                        gtgGTTTCCGGTGGCACCGACAACCACCTGTTCCTGGTCAGCCTGATCCGTCAGGGCCTGACTGGCAAAGACGCCGACGCCGCCCTGGGTCGTGCGCATATCACCGTCAACAAGAACGCCGTGCCGAACGACCCGCAGTCGCCGTTCGt  >  6:258201/1‑149 (MQ=255)
                                                                                                                                        gtgGTTTCCGGTGGCACCGACAACCACCTGTTCCTGGTCAGCCTGATCCGTCAGGGCCTGACTGGCAAAGACGCCGACGCCGCCCTGGGTCGTGCGCATATCACCGTCAACAAGAACGCCGTGCCGAACGACCCGCAGTCGCCGTTCGt  >  2:176978/1‑149 (MQ=255)
                                                                                                                                        gtgGTTGTCGGTGCCACCGGAAACCACCTGTTCCTGGTCAGCCTGATCCGTCAGGGCCTGACTGGCAAAGACGCCGACGCCGCCCTGGGTCGTGCGCATATCACCGTCAACAAGAACGCCGTGCCGAACGACCCGCAGTCGCCGTTCGt  >  5:12662/1‑149 (MQ=255)
                                                                                                                                               |                                                                                                                                             
CCCGCTGATGCACGTGATCGCTGCCAAGGCAGTGTGCTTCAAGGAAGCGCTGGAGCCTGGATTCAAGGCCTACCAGCAGCAAGTGATCGAAAACGCCCAGGCCATGGCCCAGGTGTTCATCGACCGTGGCTACGATGTGGTTTCCGGTGGCACCGACAACCACCTGTTCCTGGTCAGCCTGATCCGTCAGGGCCTGACTGGCAAAGACGCCGACGCCGCCCTGGGTCGTGCGCATATCACCGTCAACAAGAACGCCGTGCCGAACGACCCGCAGTCGCCGTTCGT  >  NC_002947/387649‑387933

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑