Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,855,011 A→C 6.1% N30K (AAT→AAG)  dnaX ← DNA polymerase III subunit gamma

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,855,0110AC 6.1% 186.8 / 2.2 61N30K (AAT→AAG) dnaXDNA polymerase III subunit gamma
Reads supporting (aligned to +/- strand):  ref base A (25/31);  new base C (2/2);  total (28/33)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CACCGAACAGGTGCCACAGGGCGTGGAGGTGATGCCGGTTTCACAGTTCAGGCACTTGGCGATAATACGGGCGATGGTCGTCTTGCCCACCCCGCGGGTACCGGTGAACAGGTAGGCATGGTGCAGGCGCTGGTTGTCCAAGGCATTGATCAAGGCCTTGAGCACATGGGTCTGGCCGACCATTTCGCGGAACGAGCGCGGACGCCATTTTCGTGCAAGAACCTGATAACTCATCGAAAAACCATCGTAGCCTGATCGAGCGGAAGATCGCTAATGCTAGCGGAGCGGGGGC  >  NC_002947/4854867‑4855158
                                                                                                                                                |                                                                                                                                                   
cacCGAACAGGTGCCACAGGGCGTGGAGGTGATGCCGGTTTCACAGTTCAGGCACTTGGCGATAATACTGTCGATGGTCGTCTTGCCCAGCCCGCGGGTACCGGTGAACAGGTAGGCATGGTGCAGGCGCTGGTTGTCCAAGGCATTgt                                                                                                                                                 <  7:239017/149‑2 (MQ=255)
cacCGAACAGGTGCCACAGGGCGTGGAGGTGATGCCGGTTTCACAGTTCAGGCACTTGGCGATAATACGGGCGATGGTCGTCTTGCCCACCCCGCGGGTACCGGTGAACAGGTAGGCATGGTGCAGGCGCTGGTTGTCCAAGGCATTGa                                                                                                                                                 <  4:173782/149‑1 (MQ=255)
 acCGAACAGGTGCCACAGGGCGTGGAGGTGATGCCGGTTTCACAGTTCAGGCACTTGGCGATAATACGGGCGATGGTCGTCTTGCCCACCCCGCGGGTACCGGTGAACAGGTAGGCATGGTGCAGGCGCTGGTTGTCCAAGGCATTGAt                                                                                                                                                >  5:207689/1‑149 (MQ=255)
 acCGAACAGGTGCCACAGGGCGTGGAGGTGATGCCGGTTTCACAGTTCAGGCACTTGGCGATAATACGGGAGATGGTCGTCTTGCCCACCCATCGGGTACAGGTGAACAGGAAGGAATGGTGCAGGCGCTGGTTGTCCAAGCCAttca                                                                                                                                                 >  8:174956/1‑146 (MQ=255)
  ccGAACAGGTGCCACAGGGCGTGGAGGTGATGCCGGTTTCACAGTTCAGGCACTTGGCGATAATACGGGCGATGGTCGTCTTGCCCACCCCGCGGGTACCGGTGAACAGGTAGGCATGGTGCAGGCGCTGGTTGTCCAAGGCATTGATc                                                                                                                                               <  3:361875/149‑1 (MQ=255)
  ccGAACAGGTGCCACAGGGCGTGGAGGTGATGCCGGTTTCACAGTTCAGGCACTTGGCGATAATACGGGCGATGGTCGTCTTGCCCACCCCGCGGGTACCGGTGAACAGGTAGGCATGGTGCAGGCGCTGGTTGTCCAAGGCATTGATc                                                                                                                                               <  7:75880/149‑1 (MQ=255)
   cGAACAGGTGCCACAGGGCGTGGAGGTGATGCCGGTTTCACAGTTCAGGCACTTGGCGATAATACGGGCGATGGTCGTCTTGCCCACCCCGCGGGTACCGGTGAACAGGTAGGCATGGTGCAGGCGCTGGTTGTCCAAGGCATTGATCa                                                                                                                                              <  3:108118/149‑1 (MQ=255)
    gAACAGGTGCCACAGGGCGTGGAGGTGATGCCGGTTTCACAGTTCAGGCACTTGGCGATAATACGGGCGATGGTCGTCTTGCCCACCCCGCGGGTACCTGTGAACAGGTAGGCATGGTGCAGGCGCTGGTTGTCCAAGGCATTGATc                                                                                                                                               >  5:376917/1‑147 (MQ=255)
     aaCAGGTGCCACAGGGCGTGGAGGTGATGCCGGTTTCACAGTTCAGGCACTTGGCGATAATACGGGCGATGGTCGTCTTGCCCACCCCGCGGGTACCGGTGAACAGGTAGGCATGGTGCAGGCGCTGGTTGTCCAAGGCATTGATCAAg                                                                                                                                            <  5:365979/149‑1 (MQ=255)
        aGGTGCCACAGGGCGTGGAGGTGATGCCGGTTTCACAGTTCAGGCACTTGGCGATAATACGGGCGATGGTCGTCTTGCCCACCCCGCGGGTACCGGTGAACAGGTAGGCATGGTGCAGGCGCTGGTTGTCCAAGGCCTTGATCAATGcc                                                                                                                                         >  6:223275/1‑149 (MQ=255)
          gTGCCACAGGGCGTGGAGGTGATGCCGGTTTCACAGTTCAGGCACTTGGCGATAATACGGGCGATGGTCGTCTTGCCCACCCCGCGGGTACCGGTGAACAGGTAGGCATGGTGCAGGCGCTGGTTGTCCAAGGCCTTGATCAATGCCtt                                                                                                                                       <  5:223275/149‑1 (MQ=255)
          gTGCCACAGGGCGTGGAGGTGATGCCGGTTTCACAGTTCAGGCACTTGGCGATAATACGGGCGATGGTCGTCTTGCCCACCCCGCGGGTACCGGTGAACAGGTAGGCATGGTGCAGGCGCTGGTTGTCCAAGGCATTGATCAAGGCCtt                                                                                                                                       <  3:75998/149‑1 (MQ=255)
              cacaGGGCGTGGAGGTGATGCCGGTTTCACAGTTCAGGCACTTGGCGATAATACGGGCGATGGTCGTCTTGCCCACCCCGCGGGTACCGGTGAACAGGTAGGCATGGTGCAGGCGCTGGTTGTCCAAGGCATTGATCAAGGCCTTGAg                                                                                                                                    >  8:278385/1‑148 (MQ=255)
                  gggCGTGGAGGTGATGCCGGTTTCACAGTTCAGGCACTTGGCGATAATACGGGCGATGGTCGTCTTGCCCACCCCGCGGGTACCGGTGAACAGGTAGGCATGGTGCAGGCGCTGGTTGTCCAAGGCATTGATCAAGGCCTTGAGCACAt                                                                                                                               <  7:280172/149‑1 (MQ=255)
                   ggCGTGGAGGTGATGCCGGTTTCACAGTTCAGGCACTTGGCGATAATACGGGCGATGGTCGTCTTGCCCACCCCGCGGGTACCGGTGAACAGGTAGGCATGGTGCAGGCGCTGGTTGTCCAAGGCATTGATCaa                                                                                                                                             <  8:178671/134‑1 (MQ=255)
                   ggCGTGGAGGTGATGCCGGTTTCACAGTTCAGGCACTTGGCGATAATACGGGCGATGGTCGTCTTGCCCACCCCGCGGGTACCGGTGAACAGGTAGGCATGGTGCAGGCGCTGGTTGTCCAAGGCATTGATCaa                                                                                                                                             >  7:178671/1‑134 (MQ=255)
                       tGGAGGTGATGCCGGTTTCACAGTTCAGGCACTTGGCGATAATACGGGCGATGGTCGTCTTGCCCACCCCGCGGGTACCGGTGAACAGGTAGGCATGGTGCAGGCGCTGGTTGTCCAAGGCATTGATCAAGGCCTTGAGCACATGGGTc                                                                                                                          >  1:122168/1‑149 (MQ=255)
                                 gCCGGTTTCACAGTTCAGGCACTTGGCGATAATACGGGCGATGGTCGTCTTGCCCACCCCGCGGGTACCGGTGAACAGGTAGGCATGGTGCAGGCGCTGGTTGTCCAAGGCAt                                                                                                                                                    >  3:238689/1‑113 (MQ=255)
                                 gCCGGTTTCACAGTTCAGGCACTTGGCGATAATACGGGCGATGGTCGTCTTGCCCACCCCGCGGGTACCGGTGAACAGGTAGGCATGGTGCAGGCGCTGGTTGTCCAAGGCAt                                                                                                                                                    <  4:238689/113‑1 (MQ=255)
                                 gCCGGTTTCACAGTTCAGGCACTTGGCGATAATACGGGCGATGGTCGTCTTGCCCACCCCGCGGGTACCGGTGAACAGGTAGGCATGGTGCAGGCGCTGGTTGTCAAAGGCTTTGATCAAGGCATAGAGCACATGGTTCTGGCCGACCa                                                                                                                >  8:121108/1‑149 (MQ=255)
                                     gTTTCACAGTTCAGGCACTTGGCGATAATACGGGCGATGGTCGTCTTGCCCACCCCGCGGGTACCGGTGAACAGGTAGGCATGGTGCAGGCGCTGGTTGTCCAAGGCATTGATCAAGGCCTTGAGCACATGGGTCTGGCCGACCATTTc                                                                                                            >  5:207702/1‑149 (MQ=255)
                                         cacaGTTCAGGCACTTGGCGATAATACGGGCGATGGTCGTCTTGCCCACCCCGCGGGTACCGGTGAACAGGTAGGCATGGTGCAGGCGCTGGTTGTCCAAGGCATTGATCAAGGCCTTGAGCACATGGGTCTGGCCGACCATTTcgcg                                                                                                         <  1:47040/148‑1 (MQ=255)
                                          acaGTTCAGGCACTTGGCGATAATACGGGCGATGGTCGTCTTGCCCACCCCGCGGGTACCGGTGAACAGGTAGGCATGGTGCAGGCGCTGGTTGTCCAAGGCATTGATCAAGGCCTTGAGCACATGGGTCTGGCCGACCATTTCGCgg                                                                                                        <  8:282849/148‑1 (MQ=255)
                                                  ggCACTTGGCGATAATACGGGCGATGGTCGTCTTGCCCACCCCGCGGGTACCGGTGAACAGGTAGGCATGGTGCAGGCGCTGGTTGTCCAAGGCATTGATCAAGGCCTTGAGCACATGGGTCTGGCCGACCATTTCGCGGAACGAgcgc                                                                                               <  3:1382/149‑1 (MQ=255)
                                                      cTTGGCGATAATACGGGCGATGGTCGTCTTGCCCACCCCGCGGGTACCGGTGAACAGGTAGGCATGGTGCAGGCGCTGGTTGTCCAAGGCATTGATCAAGGCCTTGAGCACATGGGTCTGGCCGACCATTTCGCGGAACGAGCGCGGAc                                                                                           <  7:7430/149‑1 (MQ=255)
                                                       ttGGCGATAATACGGGCGATGGTCGTCTTGCCCACCCCGCGGGTACCGGTGAACAGGTAGGCATGGTGCAGGCGCTGGTTGTCCAAGGCATTGATCAAGGCCTTGAGCACATGGGTCTGGCCGCCCATTTCGCGGAACGAGCGCGGACg                                                                                          >  2:304238/1‑149 (MQ=255)
                                                       ttGGCGATAATACGGGCGATGGTCGTCTTGCCCACCCCGCGGGTACCGGTGAACAGGTAGGCATGGTGCAGGCGCTGGTTGTCCAAGGCATTGATCAAGGCCTTGAGCACATGGGTCTGGCCGACCATTTCGCGGAACGAGCGCGGACg                                                                                          >  5:290923/1‑149 (MQ=255)
                                                             ataataCGGGCGATGGTCGTCTTGCCCACCCCGCGGGTACCGGTGAACAGGTAGGCATGGTGCAGGCGCTGGTTGTCCAAGGCATTGATCAAGGCCTTGAGCACATGGGTCTGGCCGACCATTTCGCGGAACGAGCGCGGACGCCAttt                                                                                    >  5:332605/1‑149 (MQ=255)
                                                                 taCGGGCGATGGTCGTCTTGCCCACCCCGCGGGTACCGGTGAACAGGTAGGCATGGTGCAGGCGCTGGTTGTCCAAGGCATTGATCAAGGCCTTGAGCACATGGGTCTGGCCGACCATTTCGCGGAACGAGCGCGGACGCCATTTTCGt                                                                                <  2:246119/149‑1 (MQ=255)
                                                                 taCGGGCGATGGTCGTCTTGCCCACCCCGCGGGTACCGGTGAACAGGTAGGCATGGTGCAGGCGCTGGTTGTCCAAGGCATTGATCAAGGCCTTGAGCACATGGGTCTGGCCGACCATTTCGCGGAACGAGCGCGGACGCCATTTTCGt                                                                                >  7:21627/1‑149 (MQ=255)
                                                                 taCGGGCGATGGTCGTCTTGCCCACCCCGCGGGTACCGGTGAACAGGTAGGCATGGTGCAGGCGCTGGTTGTCCAAGGCATTGATCAAGGCCTTGAGCACATGGGTCTGGCCGACCATTTCGCGGAACGAGCGCGGACGCCATTTTCGt                                                                                <  8:21627/149‑1 (MQ=255)
                                                                   cGGGCGATGGTCGTCTTGCCCACCCCGCGGGTACCGGTGAACAGGTAGGCATGGTGCAGGCGCTGGTTGTCCAAGGCATTGATCAAGGCCTTGAGCACATGGGTCTGGCCGACCATTTCGCGGAACGAGCGCGGACGCCATTTTCGTg                                                                               <  2:122168/148‑1 (MQ=255)
                                                                         aTGGTCGTCTTGCCCACCCCGCGGGTACCGGTGAACAGGTAGGCATGGTGCAGGCGCTGGTTGTCCAAGGCATTGATCAAGGCCTTGAGCACATGGGTCTGGCCGACCATTTCGCGGAACGAGCGCGGACGCCATTTTCGTGCAAGAAc                                                                        <  6:207702/149‑1 (MQ=255)
                                                                             tcgtcTTGCCCACCCCGCGGGTACCGGTGAACAGGTAGGCATGGTGCAGGCGCTGGTTGTCCAAGGCATTGATCAAGGCCTTGAGCACATGGGTCTGGCCGACCATTTCGCGGAACGAGCGCGGACGCCATTTTCGTGCAAGAACCTGa                                                                    <  7:278385/149‑1 (MQ=255)
                                                                               gtcTTGCCCACCCCGCGGGTACCGGTGAACAGGTAGGCATGGTGCAGGCGCTGGTTGTCCAAGGCATTGATCAAGGCCTTGAGCACATGGGTCTGGCCGACCATTTCGCGGAACGAGCGCGGACGCCATTTTCGTGCAAGAACCTGAt                                                                   >  1:353468/1‑148 (MQ=255)
                                                                                tcTTGCCCACCCCGCGGGTACCGGTGAACAGGTAGGCATGGTGCAGGCGCTGGTTGTCCAAGGCATTGATCAAGGCCTTGAGCACATGGGTCTGGCCGACCATTTCGCGGAACGAGCGCGGACGCCATTTTCGTGCAAGAACCTGATaa                                                                 >  4:267988/1‑149 (MQ=255)
                                                                                tcTTGCCCACCCCGCGGGTACCGGTGAACAGGTAGGCATGGTGCAGGCGCTGGTTGTCCAAGGCATTGATCAAGGCCTTGAGCACATGGGTCTGGCCGACCATTTCGCGGAACGAGCGCGGACGCCATTTTCGTACAAGAACCTGATaa                                                                 >  1:128342/1‑149 (MQ=255)
                                                                                      ccACCCCGCGGGTACCGGTGAACAGGTAGGCATGGTGCAGGCGCTGGTTGTCCAAGGCATTGATCaa                                                                                                                                             <  6:106531/67‑1 (MQ=255)
                                                                                      ccACCCCGCGGGTACCGGTGAACAGGTAGGCATGGTGCAGGCGCTGGTTGTCCAAGGCATTGATCaa                                                                                                                                             >  5:106531/1‑67 (MQ=255)
                                                                                      ccACCCCGCGGGTACCGGTGAACAGGTAGGCATGGTGCAGGCGCTGGTTGTCCAAGGCATTGATCAAGGCCTTGAGCACATGGGTCTGGCCGACCATTTCGCGGAACGAGCGCGGACGCCATTTTCGTGCAAGAACCTGATAACTCATc                                                           <  2:353468/149‑1 (MQ=255)
                                                                                            cgcgGGTACCGGTGAACAGGTAGGCATGGTGCAGGCGCTGGTTGTCCAAGGCATTGATCAAGGCCTTGAGCACATGGGTCTGGCCGACCATTTCGCGGAACGAGCGCGGACGCCATTTTCGTGCAAGAACCTGATAACTCATCGaaaaa                                                     >  2:339942/1‑149 (MQ=255)
                                                                                                ggTACCGGTGAACAGGTAGGCATGGTGCAGGCGCTGGTTGTCCAAGGCATTGATCAAGGCCTTGAGCACATGGGTCTGGCCGACCATTTCGCGGAACGAGCGCGGACGCCATTTTCGTGCAAGAACCTGATAACTCATCGAAAAACCAt                                                 >  4:303305/1‑149 (MQ=255)
                                                                                                      ggTGAACAGGTAGGCATGGTGCAGGCGCTGGTTGTCCAAGGCATTGATCAAGGCCTTGAGCACATGGGTCTGGCCGACCATTTCGCGGAACGAGCGCGGACGCCATTTTCGTGCAAGAACCTGATAACTCATCGAAAAACCATCGTAGc                                           <  6:207689/149‑1 (MQ=255)
                                                                                                       gTGAACAGGTAGGCATGGTGCAGGCGCTGGTTGTCCAAGGCATTGATCAAGGCCTTGAGCACATGGGTCTGGCCGACCATTTCGCGGAACGAGCGCGGACGCCATTTTCGTGCAAGAACCTGATAACTCATCGAAAAACCATCGTAGcc                                          >  4:165463/1‑149 (MQ=255)
                                                                                                                tAGGCATGGTGCAGGCGCTGGTTGTCCAAGGCATTGATCAAGGCCTTGAGCACATGGGTCTGGCCGACCATTTCGCGGAACGAGCGCGGACGCCATTTTCGTGCAAGAACCTGATAACTCATCGAAAAACCATCGTAGCCTGATCGAGc                                 <  6:332605/149‑1 (MQ=255)
                                                                                                                  ggCATGGTGCAGGCGCTGGTTGTCCAAGGCATTGATCAAGGCCTTGAGCACATGGGTCTGGCCGACCATTTCGCGGAACGAGCGCGGACGCCATTTTCGTGCAAGAACCTGATAACTCATCGAAAAACCATCGTAGCCTGATCGAGCgg                               <  3:267988/149‑1 (MQ=255)
                                                                                                                    cATGGTGCAGGCGCTGGTTGTCCAAGGCATTGATCAAGGCCTTGAGCACATGGGTCTGGCCGACCATTTCGCGGAACGAGCGCGGACGCCATTTTCGTGCAAGAACCTGATAACTCATCGAAAAACCATCGTAGCCTGATCGAGCGGaa                             <  3:303305/149‑1 (MQ=255)
                                                                                                                     aTGGTGCAGGCGCTGGTTGTCCAAGGCATTGATCAAGGCCTTGAGCACATGGGTCTGGCCGACCATTTCGCGGAACGAGCGCGGACGCCATTTTCGTGCAAGAACCTGAt                                                                   <  2:306744/110‑1 (MQ=255)
                                                                                                                        gTGCAGGCGCTGGTTGTCCAAGGCATTGATCAAGGCCTTGAGCACATGGGTCTGGCCGACCATTTCGCGGAACGAGCGCGGACGCCATTTTCGTACAAGAACCTGATAACTCATCGAAAAACCATCGTAGCCTGATCGAGCGGAAGATc                         <  2:128342/149‑1 (MQ=255)
                                                                                                                           cAGGCGCTGGTTGTCCAAGGCATTGATCAAGGCCTTGAGCACATGGGTCTGGCCGACCATTTCGCGGAACGAGCGCGGACGCCATTTTCTTGCAAGAACCTGATAACTCATCGAAAAACCATCGTAGCCTGATCGAGCGGAAGATCGc                       >  4:87426/1‑148 (MQ=255)
                                                                                                                           cAGGCGCTGGTTGTCCAAGGCATTGATCAAGGCCTTGAGCACATGGGTCTGGCCGACCATTTCGCGGAACGAGCGCGGACGCCATTTTCGTGCAAGAACCTGATAACTCATCGAAAAACCATCGTAGCCTGATCGAGCGGAAGATCGc                       <  6:376917/148‑1 (MQ=255)
                                                                                                                             ggCGCTGGTTGTCCAAGGCATTGATCAAGGCCTTGAGCACATGGGTCTGGCCGACCATTTCGCGGAACGAGCGCGGACGCCATTTTCGTGCAAGAACCTGATAACTCATCGAAAAACCATCGTAGCCTGATCGAGCGGAAGATCGCTaa                    >  4:351125/1‑149 (MQ=255)
                                                                                                                                    gTTGTCCAAGGCATTGATCAAGGCCTTGAGCACATGGGTCTGGCCGACCATTTCGCGGAACGAGCGCGGCCGCCATTTTCGTGCAAGAACCTGATAACTCATCGAAAAACCATCGTAGCCTGATCGAGCGGAAGATCGCTAATGCTagc             >  8:279319/1‑149 (MQ=255)
                                                                                                                                     ttGTCCAAGGCATTGATCAAGGCCTTGAGCACATGGGTCTGGCCGACCATTTCGCGGAACGAGCGCGGACGCCATTTTCGTGCAAGAACCTGATAACTCATCGAAAAACCATCGTAGCCTGATCGAGCGGAAGATCGCTAATGCTagcg            <  7:121108/149‑1 (MQ=255)
                                                                                                                                      tgctcAAGGCCTTGATCAATGCCTTGAGCACATGGGTCTGGCCGACCATTTCGCGGAACGAGCGCGGACGCCATTTTCGTGCAAGAACCTGATAACTCATCGAAAAACCATCGTAGCCTGATCGAGCGGAAGATCGc                       <  5:156530/133‑1 (MQ=255)
                                                                                                                                      tgctcAAGGCCTTGATCAATGCCTTGAGCACATGGGTCTGGCCGACCATTTCGCGGAACGAGCGCGGACGCCATTTTCGTGCAAGAACCTGATAACTCATCGAAAAACCATCGTAGCCTGATCGAGCGGAAGATCGc                       >  6:156530/5‑137 (MQ=255)
                                                                                                                                       gTCCAAGGCATTGATCAAGGCCTTGAGCACATGGGTCTGGCCGACCATTTCGCGGAACGAGCGCGGACGCCATTTTCGTGCAAGAACCTGATAACTCATCGAAAAACCATCGTAGCCTGATCGAGCGGAAGATCGCTAATGCTagcg            >  2:318741/1‑147 (MQ=255)
                                                                                                                                        tCCAAGGCATTGATCAAGGCCTTGAGCACATGGGTCTGGCCGACCATTTCGCGGAACGAGCGCGGACGCCATTTTCGTGCAAGAACCTGATAACGCATCGAAAAACCATCGTAGCCTGATCGAGCGGAAGATCGCTAATGCTagcggag         >  6:91569/1‑149 (MQ=255)
                                                                                                                                           aaGGCATTGATCAAGGCCTTGAGCACATGGGTCTGGCCGACCATTTCGCGGAACGAGCGCGGACGCCATTTTCGTGCAAGAACCTGATAACTCATCGAAAAACCATCGTAGCCTGATCGAGCGGAAGATCGCTAATGCTagcggagcgg      >  6:265980/1‑149 (MQ=255)
                                                                                                                                            aGGCATTGATCAAGGCCTTGAGCACATGGGTCTGGCCGACCATTTCGCGGAACGAGCGCGGACGCCATTTTCGTGCAAGAACCTGATAACTCATCGAAAAACCATCGTAGCCTGATCGAGCGGAAGATCGCTAATGCTAGCGGAGCggg     <  3:351125/149‑1 (MQ=255)
                                                                                                                                             ggCATTGATCAAGGCCTTGAGCACATGGGTCTGGCCGACCATTTCTCGTAACGAGCGCGGACGCCATTTTCGTGCAAGAACCTGATAACTCATCGAAAAACCATCGTAGCCTTATCGAGCGGAAGATCGCTAATGCTAGCGGAGCgggg    <  7:174956/149‑1 (MQ=255)
                                                                                                                                                aTTGATCAAGGCCTTGAGCACATGGGTCTGGCCGACCATTTCGCGGAACGAGCGCGGACGCCATTTTCGTGCAAGAACCTGATAACTCATCGAAAAACCATCGTAGCCTGATCGAGCGGAAGATCGCTAATGCTagcggagcgg      <  8:29996/144‑1 (MQ=255)
                                                                                                                                                aTTGATCAAGGCCTTGAGCACATGGGTCTGGCCGACCATTTCGCGGAACGAGCGCGGACGCCATTTTCGTGCAAGAACCTGATAACTCATCGAAAAACCATCGTAGCCTGATCGAGCGGAAGATCGCTAATGCTagcggagcgg      >  7:29996/1‑144 (MQ=255)
                                                                                                                                                aTTGATCAAGGCCTTGAGCACATGGGTCTGGCCGACCATTTCGCGGAACGAGCGCGGACGCCATTTTCGTGCAAGAACCTGATAACTCATCGAAAAACCATCGTAGCCTGATCGAGCGGAAGATCGCTAATGCTAGCGGAGCGGGGGc  >  2:112784/1‑148 (MQ=255)
                                                                                                                                                aTTGATCAAGGCCTTGAGCACATGGGTCTGGCCGACCATTTCGCGGAACGAGCGCGGACGCCATTTTCGTGCAAGAACCTGATAACTCATCGAAAAACCATCGTAGCCTGATCGAGCGGAAGATCGCTAATGCTAGCCGAGCGGGGGc  >  2:316349/1‑148 (MQ=255)
                                                                                                                                                |                                                                                                                                                   
CACCGAACAGGTGCCACAGGGCGTGGAGGTGATGCCGGTTTCACAGTTCAGGCACTTGGCGATAATACGGGCGATGGTCGTCTTGCCCACCCCGCGGGTACCGGTGAACAGGTAGGCATGGTGCAGGCGCTGGTTGTCCAAGGCATTGATCAAGGCCTTGAGCACATGGGTCTGGCCGACCATTTCGCGGAACGAGCGCGGACGCCATTTTCGTGCAAGAACCTGATAACTCATCGAAAAACCATCGTAGCCTGATCGAGCGGAAGATCGCTAATGCTAGCGGAGCGGGGGC  >  NC_002947/4854867‑4855158

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑