Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,855,539 T→G 8.0% I106M (ATT→ATG)  PP_4270 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,855,5390TG 8.0% 150.8 / 5.4 50I106M (ATT→ATG) PP_4270hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (23/23);  new base G (2/2);  total (25/25)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.56e-01

GGCGCTCGCCCCGAGTTCCACGTCATGGCCCGCCTGCGCCTGGAGGAGGCCATGAGCAGCGGCGATATTGATGTGCGTTGCTACGTCAGTACCCAGTGGCTCAACGACCGTCCAAGGGATTTTGTGTGGAGTATCCCGCTGATTCATCAGCGCGACGTACTGGTTGGCCGCCCCGGCGACAGCACCCCGATGCCCCCGGAGCAACTGCCACCCCAGGCGATCGGCACGGTGCTGGGCTACACCTATTCAACCCTGCAACCACTGCTGGACCACGGCCAGTTGCACCGTGAG  >  NC_002947/4855396‑4855686
                                                                                                                                               |                                                                                                                                                   
ggCGCTCGCCCCGAGTTCCACGTCATGGCCCGCCTGCGCCTGGAGGAGGCCATGAGCAGCGGCGATATTGATGTGCGTTGCTACGTCAGTACCCAGTGGCTCAACGACCGTCCAAGGGATTTTGTGTGGAGTATCCCGCTGATtcatc                                                                                                                                                 <  6:331832/148‑1 (MQ=255)
   gcTCGCCCCGAGTTCCACGTCATGGCCCGCCTGCGCCTGGAGGAGGCCATGAGCAGCGGCGATATTGATGTGCGTTGCTACGTCAGTACCCAGTGGCTCAACGACCGTCCAAGGGATTTTGTGTGGAGTATCCCGCTGATTCATCAgcg                                                                                                                                             >  5:348897/1‑149 (MQ=255)
    cTCGCCCCGAGTTCCACGTCATGGCCCGCCTGCGCCTGGAGGAGGCCATGAGCAGCGGCGATATTGATGTGCGTTGCTACGTCAGTACCCAGTGGCTCAACGACCGTCCAAGGGATTTTGTGTGGAGTATCCCGCTGATGAATCAgcgg                                                                                                                                            <  2:367287/149‑2 (MQ=255)
          ccGAGTTCCACGTCATGGCCCGCCTGCGCCTGGAGGAGGCCATGAGCAGCGGCGATATTGATGTGCGTTGCTACGTCAGTACCCAGTGGCTCAACGACCGTCCAAGGGATATTGTGTGGAGTATCCCGCAGATTCATCAGCGCGACg                                                                                                                                        >  5:150576/1‑147 (MQ=255)
                tCCACGTCATGGCCCGCCTGCGCCTGGAGGAGGCCATGAGCAGCGGCGATATTGATGTGCGTTGCTACGTCAGTACCCAGTGGCTCAACGACCGTCCAAGGGATTTTGTGTGGAGTATCCCGCTGATTCATCAGCGCGACGTACTGGtt                                                                                                                                >  6:44537/1‑149 (MQ=255)
                 ccACGTCATGGCCCGCCTGCGCCTGGAGGAGGCCATGAGCAGCGGCGATATTGATGTGCGTTGCTACGTCAGTACCCAGTGGCTCAACGACCGTCCAAGGGATTTTGTGTGGAGTATCCCGCTGATTCATCAGCGCGACGTACTGGtt                                                                                                                                <  4:314655/148‑1 (MQ=255)
                  cACGTCATGGCCCGCCTGCGCCTGGAGGAGGCCATGAGCAGCGGCGATATTGATGTGCGTTGCTACGTCAGTACCCAGTGGCTCAACGACCGTCCAAGGGATTTTGTGTGGAGTATCCCGCTGATTCATCAGCGCGACGTACTGGTTg                                                                                                                               <  1:105424/148‑1 (MQ=255)
                           gccgtcctgtgcatgTATGAGGCCATGAGGAGCGGATATTTTGATGTGCGTTGCTACGTCAGTAGCCAGTGGCTCATCGACGGTCCAAGTGATTTTGTGTGGAGTTTCCCGCTGATTCTTCAGCTCGACGTACTGGTTGGGCGCCGCgg                                                                                                                     <  6:150576/144‑1 (MQ=255)
                                     gcctgGAGGAGGCCATGAGCAGCGGCGATATTGCTGGGCGTTGCTACGGCAGTACCCAGTGGCTCCACGACCGTCCACGGGCTTTTGTGTGGAGTATCCCGCTGATTCATCAGCGCGACGTACTGGGTGGCCGCCCCGGCGACAGCAcc                                                                                                           <  3:233156/149‑1 (MQ=255)
                                      cctgGAGGAGGCCATGAGCAGCGGCGATATTGATGTGCGTTGCTACGTCAGTACCCAGTGGCTCAACGACCGTCCAAGGGATTTTGTGTTGAGTATCCCGCTGATTCATCAGCGCGACGTACTgg                                                                                                                                  >  8:297732/1‑125 (MQ=255)
                                      cctgGAGGAGGCCATGAGCAGCGGCGATATTGATGTGCGTTGCTACGTCAGTACCCAGTGGCTCAACGACCGTCCAAGGGATTTTGTGTGGAGTATCCCGCTGATTCATCAGCGCGACGTACTGGTTGGCCGCCCCGGCGACAGCAccc                                                                                                          <  1:208178/149‑1 (MQ=255)
                                      cctgGAGGAGGCCATGAGCAGCGGCGATATTGATGTGCGTTGCTACGTCAGTACCCAGTGGCTCAACGACCGTCCAAGGGATTTTGTGTCGAGTATCCCGCTGATTCATCAGCGCGACGTACTgg                                                                                                                                  <  7:297732/125‑1 (MQ=255)
                                          gaggagGCCATGAGCAGCGGCGATATTGATGTGCGTTGCTACGTCAGTACCCAGTGGCTCAACGACCGTCCAAGGGATTTTGTGTGGAGTATCCCGCTGATTCATCAGCGCGACGTACTGGTTGGCCGCCCCGGCGACAGCACCCCGAt                                                                                                      >  7:204911/1‑149 (MQ=255)
                                          gaggagGCCATGAGCAGCGGCGATATTGATGTGCGTTGCTACGTCAGTACCCAGTGGCTCAACGACCGTCCAAGGGATTTTGTGTGGAGTATCCCGCTGATTCATCAGCGCGACGTACTGGTTGGCCGCCCCGGCGACAGCACCCCGAt                                                                                                      <  6:348897/149‑1 (MQ=255)
                                            ggaggCCATGAGCAGCGGCGATATTGATGTGCGTTGCTACGTCAGTACCCAGTGGCTCAACGACCGTCCAAGGGATTTTGTGTGGAGTATCCCGCTGATTCATCAGCGCGACGTACTGGTTGGCCGCCCCGGCGACAGCACCCCGATGc                                                                                                    >  8:258653/1‑149 (MQ=255)
                                               ggCCATGAGCAGCGGCGATATTGATGTGCGTTGCTACGTCAGTACCCAGTGGCTCAACGACCGTCCAAGGGATTTTGTGTGGAGTATCCCGCTGATTCATCAGCGCGAAGTACTGGTTGGCCGCCCCGTCGACAGCACCACGATGcccc                                                                                                 >  2:34773/1‑149 (MQ=255)
                                                  cATGAGCAGCGGCGATATTGATGTGCGTTGCTACGTCAGTACCCAGTGGCTCAACGACCGTCCAAGGGATTTTGTGTGGAGTATCCCGCTGATTCATCAgc                                                                                                                                              <  2:205170/101‑1 (MQ=255)
                                                  cATGAGCAGCGGCGATATTGATGTGCGTTGCTACGTCAGTACCCAGTGGCTCAACGACCGTCCAAGGGATTTTGTGTGGAGTATCCCGCTGATTCATCAgc                                                                                                                                              >  1:205170/1‑101 (MQ=255)
                                                       gcagcGGCGATATTGATGTGCGTTGCTACGTCAGTACCCAGTGGCTCAACGTCCGTCCAAGGGATTTTGTGTGGAGTATCCCGCTGATTCATCAGCGCGACGTACTGGTTGGCCGCCCCGGCGACAGCACCCCGATGCCCCCGGAGCaa                                                                                         <  2:47544/149‑1 (MQ=255)
                                                        cagcGGCGATATTGATGTGCGTTGCTACGTCAGTACCCAGTGGCTCAACGACCGTCCAAGGGATTTTGTGTGGAGTATCCCGCTGATGAATCAGCGGGa                                                                                                                                          >  3:123260/1‑99 (MQ=255)
                                                        cagcGGCGATATTGATGTGCGTTGCTACGTCAGTACCCAGTGGCTCAACGACCGTCCAAGGGATTTTGTGTGGAGTATCCCGCTGATGAATCAGCGGGa                                                                                                                                          <  4:123260/99‑1 (MQ=255)
                                                                   ttGATGTGCGTTGCTACGTCAGTACCCAGTGGCTCAACGACCGTCCAAGGGATTTTGTGTGGAGTATCCCGCTGATTCATCAGCGCGACGTACTGGTTGGCCGCCCCGGCGACAGCACCCCGATGCCCCCGGAGCAACTGCCACCCCa                                                                              <  8:204911/148‑1 (MQ=255)
                                                                     gATGTGCGTTGCTACGTCAGTACCCAGTGGCTCAACGACCGTCCAAGGGATTTTGTGTGGAGTATCCCGCTGATTCATCAGCGCGACGTACTGGTTGGCCGCCCCGGCGACAGCACCCCGATGCCCCCGGAGCAACTGCCAccc                                                                                <  6:68023/144‑1 (MQ=255)
                                                                     gATGTGCGTTGCTACGTCAGTACCCAGTGGCTCAACGACCGTCCAAGGGATTTTGTGTGGAGTATCCCGCTGATTCATCAGCGCGACGTACTGGTTGGCCGCCCCGGCGACAGCACCCCGATGCCCCCGGAGCAACTGCCAccc                                                                                >  5:68023/1‑144 (MQ=255)
                                                                      aTGTGCGTTGCTACGTCAGTACCCAGTGGCTCAACGACCGTCCAAGGGATTTTGTGTGGAGTATCCCGCTGATTCATCAGCGCGACGTACTGGTTGGCCGCCCCGGCGACAGCACCCCGATGCCCCCGGAGCAACTGCCACCCCAGGCg                                                                          <  8:275268/149‑1 (MQ=255)
                                                                            gTTGCTACGTCAGTACCCAGTGGCTCAACGACCATCCAAGGGATTTTGTGTGGAGTATCCCGCTGATTCATCAGCGCGACGTACTGGTTGGCCGCCCCGGCGACAGCACCCCGATGCCCCCGGAGCAACTGCCACCCCAGGCGATCGGc                                                                    <  4:29178/149‑1 (MQ=255)
                                                                                cTACGTCAGTACCCAGTGGCTCAACGACCGTCCAAGGGATTTTGTGTGGAGTATCCCGCTGATTCATCAGCGCGACGTACTGGTTGGCCGCCCCGGCGACAGCACCCCGATGCCCCCGGAGCAACTGCCACCCCAGGCGATCGGCACg                                                                 <  1:34773/148‑1 (MQ=255)
                                                                                 tACGTCAGTACCCAGTGGCTCAACGACCGTCCAAGGGATTTTGTGTGGAGTATCCCGCTGATTCATCAGCGCGACGTACTGGTTGGCCGCCCCGGCGACAGAACCCCGATGCCCCCGGAGCAAATGCAACCCCAGGCGAGCGGCACGGt                                                               >  5:210949/1‑149 (MQ=255)
                                                                                   cGTCAGTACCCAGTGGCTCAACGACCGTCCAAGGGATTTTGTGTGGAGTATCCCGCTGATTCATCAGCGCGACGTACTGGTTGGCCGCCCCGGCGACAGCACCCCGATGCCCCCGGAGCAACTGCCACCCCAGGCGATCGGCACGGt                                                               <  5:44537/147‑1 (MQ=255)
                                                                                       aGTACCCAGTGGCTCAACGACCGTCCAAGGGATTTTGTGTGGAGTATCCCGCTGATTCATCAGCGCGACGTACTGGTTGGCCGCCCCGGCGACAGCACCCCGATGCCCCCGGAGCAACTGCCACCCCAGGCGATCGGCACGGTGCTgg                                                          <  4:366749/148‑1 (MQ=255)
                                                                                            ccAGTGGCTCAACGACCGTCCAAGGGATTTTGTGTGGAGTATCCCGCTGATTCATCAGCGCGACGTACTGGTTGGCCGCCCCGGCGACAGCACCCCGATGCCCCCGGAGCAACTGCCACCCCAg                                                                             >  4:284460/1‑124 (MQ=255)
                                                                                            ccAGTGGCTCAACGACCGTCCAAGGGATTTTGTGTGGAGTATCCCGCTGATTCATCAGCGCGACGTACTGGTTGGCCGCCCCGGCGACAGCACCCCGATGCCCCCGGAGCAACTGCCACCCCAg                                                                             <  3:284460/124‑1 (MQ=255)
                                                                                                  gCTCAACGACCGTCCAAGGGATTTTGTGTGGAGTATCCCGCTGATTCATCAGCGCGACGTACTGGTTGGCCGCCCCGGCGACAGCACCCCGATGCCCCCGGAGCAACTGCCACCCCAGGCGATCGGCACGGTGCTGGGCTACACCTAtt                                              >  8:345381/1‑149 (MQ=255)
                                                                                                   cTCAACGACCGTCCAAGGGATTTTGTGTGGAGTATCCCGCTGATTCATCAGCGCGACGTACTGGTTGGCCGCCCCGGCGACAGCACCCCGATGCCCCCGGAGCAACTGCCACCCCAGGCGATCGGCACGGTGCTGGGCTACACCTAtt                                              >  7:325176/1‑148 (MQ=255)
                                                                                                    tCAACGACCGTCCAAGGGATTTTGTGTGGAGTATCCCGCTGATTCATCAGCGCGACGTACTGGTTGGCCGCCCCGGCGACAGCACCCCGATGCCCCCGGAGCAACTGCCACCCCAGGCGATCGGCACGGTGCTg                                                           <  3:316727/134‑1 (MQ=255)
                                                                                                    tCAACGACCGTCCAAGGGATTTTGTGTGGAGTATCCCGCTGATTCATCAGCGCGACGTACTGGTTGGCCGCCCCGGCGACAGCACCCCGATGCCCCCGGAGCAACTGCCACCCCAGGCGATCGGCACGGTGCTg                                                           >  4:316727/1‑134 (MQ=255)
                                                                                                    tCAACGACCGTCCAAGGGATTTTGTGTGGAGTATCCCGCTGATTCATCAGCGCGACGTACTGGTTGGCCGCCCCGGCGACAGCACCCCGATGCCCCCGGAGCAACTGCCACCCCAGGCGATCGGCACGGTGCTGGGCTACACCTAt                                               <  2:42560/146‑1 (MQ=255)
                                                                                                    tCAACGACCGTCCAAGGGATTTTGTGTGGAGTATCCCGCTGATTCATCAGCGCGACGTACTGGTTGGCCGCCCCGGCGACAGCACCCCGATGCCCCCGGAGCAACTGCCACCCCAGGCGATCGGCACGGTGCTGGGCTACACCTAt                                               >  1:42560/1‑146 (MQ=255)
                                                                                                    tCAACGACCGTCCAAGGGATTTTGTGTGGAGTATCCCGCTGATTCATCAGCGCGACGTACTGGTTGGCCGCCCCGGCGACAGCACCCCGATGCCCCCGGAGCAACTGCCACCCCAGGCGATCGGCACGGTGCTGGGCTACACCTATTc                                             >  6:26208/1‑148 (MQ=255)
                                                                                                         gACCGTCCAAGGGATTTTGTGTGGAGTATCCCGCTGATTCATCAGCGCGACGTACTGGTTGGCCGCCCCGGCGACAGCACCCCGATGCCCCCGGAGCAACTGCCACCCCAGGCGATCGGCACGGTGCTGGGCTACACCTATTCAACCCt                                       >  2:19232/1‑149 (MQ=255)
                                                                                                                 aaGGGATTTTGTGTGGAGTATCCCGCTGATTCATCAGCGCGACGTACTGGTTGGCCGCCCCGGCGACAGCACCCCGATGCCCCCGGa                                                                                             >  2:9093/1‑87 (MQ=255)
                                                                                                                 aaGGGATTTTGTGTGGAGTATCCCGCTGATTCATCAGCGCGACGTACTGGTTGGCCGCCCCGGCGACAGCACCCCGATGCCCCCGGa                                                                                             <  1:9093/87‑1 (MQ=255)
                                                                                                                   gggATTTTGTGTGGAGTATCCCGCTGATTCATCAGCGCGACGTACTGGTTGGCCGCCCCGGCGACAGCACCCCGATGCCCCCGGAGCAACTGCCACCCCAGGCGATCGGCACGGTGCTGGGCTACACCTATTCAACCCTGCAACCActg                             >  2:357105/1‑149 (MQ=255)
                                                                                                                    ggATTTTGTGTGGAGTATCCCGCTGATTCATCAGCGCGACGTACTGGTTGGCCGCCCCGGCGACAGCACCCCGATGCCCCCGGAGCAACTGCCACCCCAGGCGATCGGCACGGTGCTGGGCTACACCTATTCAACCCTGCAACCActgc                            <  8:325176/149‑1 (MQ=255)
                                                                                                                          tgtgtgGAGTATCCCGCTGATTCATCAGCGCGACGTACTGGTTGGCCGCCCCGGCGACAGCACCCCGATGCCCCCGGAGCAACTGCCACCCCAGGCGATCGGCACGGTGCTGGGCTACACCTATTCAACCCTGCAACCACTGCTGGAc                       <  5:26208/148‑1 (MQ=255)
                                                                                                                                    gtcgcGCTGATGAATCAGCGCGACGTACTGGTTGGCCGCCCCGGCGACAGCACCCCGATGCCCCCGGAGCAACTGCCACCCCAGGCGATCGGCACGGTGCTGGGCTACACCTATTCAACCCTGCAACCACTGCTGGACCACGGCCAGtt            >  2:231075/5‑149 (MQ=255)
                                                                                                                                     tCCCGCTGATTCATCAGCGCGACGTACTGGTTGGCCGCCCCGGCGACAGCACCCCGATGCCCCCGGAGCAACTGCCACCCCAGGCGATCGGCACGGTGCTGGGCTACACCTATTCAACCCTGCAACCACTGCTGGACCACGGCCAGTTg           >  3:160772/1‑149 (MQ=255)
                                                                                                                                       ccGCTGATTCATCAGCGCGACGTACTGGTTGGCCGCCCCGGCGACAGCACCCCGATGCCCCCGGAGCAACTGCCACCCCAGGCGATCGGCACGGTGCTGGGCTACACCTATTCAACCCTGCAACCACTGCTGGACCACGGCCAGTTGCa         >  5:26587/1‑149 (MQ=255)
                                                                                                                                           taaTTCATCAGCGCGACGTACTGGTTGGCCGCCCCGGCGACAGCACCCCGATGCCCCCGGAGCAACTGCCACCCCAGGCGATCGGCACGGTGCTGGGCTACACCTATTCAACCCTGCAACCACTGCTGGACCACGGCCAGTTGCACCGt     >  6:246002/3‑149 (MQ=255)
                                                                                                                                           tGATTCATCAGCGCGACGTACTGGTTGGCCGCCCCGGCGACAGCACCCCGATGCCCCCGGAGCAACTGCCACCCCAGGCGATCGGCACGGTGCTGGGCTACACCTATTCAACCCTGCAACCActgc                            <  7:116751/126‑1 (MQ=255)
                                                                                                                                           tGATTCATCAGCGCGACGTACTGGTTGGCCGCCCCGGCGACAGCACCCCGATGCCCCCGGAGCAACTGCCACCCCAGGCGATCGGCACGGTGCTGGGCTACACCTATTCAACCCTGCAACCActgc                            >  8:116751/1‑126 (MQ=255)
                                                                                                                                              ttCATCAGCGCGACGTACTGGTTGTCCGCCCCGGCGACAGCACCCCGATGCCCCCGGAGCAACTGCCACCCCAGGCGATCGGCACGGTGCTGGGCTACACCTATTCAACCCTGCAACCACTGCTGGACCACGGCCAGTTGCACCGTGAg  <  7:345381/149‑1 (MQ=255)
                                                                                                                                               tcatcaGCGCGACGTACTGGTTGGCCGCCCCGGCGACAGCACCCCGATGCCCCCGGAGCAACTGCCACCCCAGGCGATCGGCACGGTGCTGGGCTACACCTATTCAACCCTGCAACCACTGCTGGACCACGGCCAGTTGCACTGTGAg  >  5:137094/1‑148 (MQ=255)
                                                                                                                                               |                                                                                                                                                   
GGCGCTCGCCCCGAGTTCCACGTCATGGCCCGCCTGCGCCTGGAGGAGGCCATGAGCAGCGGCGATATTGATGTGCGTTGCTACGTCAGTACCCAGTGGCTCAACGACCGTCCAAGGGATTTTGTGTGGAGTATCCCGCTGATTCATCAGCGCGACGTACTGGTTGGCCGCCCCGGCGACAGCACCCCGATGCCCCCGGAGCAACTGCCACCCCAGGCGATCGGCACGGTGCTGGGCTACACCTATTCAACCCTGCAACCACTGCTGGACCACGGCCAGTTGCACCGTGAG  >  NC_002947/4855396‑4855686

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑