Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,874,170 G→A 11.7% N121N (AAC→AAT) ‡ PP_4283 ← GntR family transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,874,1700GA11.7% 135.7 / 7.5 51N121N (AAC→AAT) ‡PP_4283GntR family transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base G (23/22);  new base A (4/2);  total (27/24)
Fisher's exact test for biased strand distribution p-value = 6.71e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.93e-01

ACCAGGCTGCGCTGGAAACTCACCAGCGGCGCATTGCCCGCCGCTTCGGCCAGCTTGAGGTGGAACTCACCGGAAAGGCGGATACCGGCACCCCGGTCACCGCACGAGAAGCTG‑TCGCGCTCTTCGCGCACCATCTGCCGCAGCTCGTTGA‑GCTGCTCGGGGGTGGCATGCTGCACCGCCAGCTCGGTGATGGCGCGTTCGACCATGCGCCGCGAGAAGAACACCTGGCGCGCCTCTTCGACGGTCGGGCTGGCCACCACCGCACCGCGGTTCGGTCGCAGCAGCACCACGCTTT  >  NC_002947/4874024‑4874318
                                                                                                                                                   |                                                                                                                                                     
aCCAGGCTGCGCTGGAAACTCACCAGCGGCGCATTGCCCGCCGCTTCGGCCAGCTTGAGGTGGAACTCACCGGAAAGGCGGATACCGGCACCCCGGTCACCGCACGAGAAGCTGATCGCGCTCTTCGCGCACCATCTGCCGCAGCTCGt                                                                                                                                                      <  1:50103/149‑1 (MQ=255)
  cAGGCTGCGCTGGAAACTCACCAGCGGCGCATTGCCCGCCGCTTCGGCCAGCTTGAGGTGGAACTCACCGGAAAGGCGGATACCGGCACCCCGGTCACCGCACGAGAAGCTG‑TCGCGCTCTTCGCGCACCATCTGCCGCAGCTCGTTGa                                                                                                                                                   <  8:16394/149‑1 (MQ=255)
  cAGGCTGCGCTGGAAACTCACCAGCGGCGCATTGCCCGCCGCTTCGGCCAGCTTGAGGTGGAACTCACCGGAAAGGCGGATACCGGCACCCCGGTCACCGCACGAGAAGCTG‑TCGCGCTCTTCGCGCACCATCTGCCGCAGCTCGTTGa                                                                                                                                                   <  7:54452/149‑1 (MQ=255)
      cTGCGCTGGAAACTCACCAGCGGCGCATTGCCCGCCGCTTCGGCCAGCTTGAGGTGGAACTCACCGGAAAGGCGGATACCGGCACCCCGGTCACCGCACGAGAAGCTG‑TCGCGCTCTTCGCGCACCATCTGCCGCAGCTCAACGA‑gctg                                                                                                                                              <  8:201527/149‑1 (MQ=255)
        gcgcTGGAAACTCACCAGCGGCGCATTGCCCGCCGCTTCGGCCAGCTTGAGGTGGAACTCACCGGAAAGGCGGATACCGGCACCCCGGTCACCGCACGAGAAGCTG‑TCGCGCTCTTCGCGCACCATCTGCCGCAGCTCGTTGA‑gctgct                                                                                                                                            >  6:67268/1‑149 (MQ=255)
           cTGGAAACTCACCAGCGGCGCATTGCCCGCCGCTTCGGCCAGCTTGAGGTGGAACTCACCGGAAAGGCGGATACCGGCACCCCGGTCACCGCACGAGAAGCTG‑TCGCGCTCTTCGCGCACCATCTGCCGCAGCTCGTTGA‑GCTGCTCgg                                                                                                                                         >  1:326665/1‑149 (MQ=255)
                aaCTCACCAGCGGCGCATTGCCCGCCGCTTCGGCCAGCTTGAGGTGGAACTCACCGGAAAGGCGGATACCGGCACCCCGGTCACCGCACGAGAAGCTG‑TCGCGCTCTTCGCGCACCATCTGCCGCAGCTCGTTGA‑GCTGCTCGGGGGTg                                                                                                                                    <  3:323793/149‑1 (MQ=255)
                    cACCAGCGGCGCATTGCCCGCCGCTTCGGCCAGCTTGAGGTGGAACTCACCGGAAAGGCGGATACCGGCACCCCGGTCACCGCACGAGAAGCTG‑TCGCGCTCTTCGCGCACCATCTGCCGCAGCTCGTTGA‑GCTGCTCGGGGGTGGCAt                                                                                                                                >  2:280333/1‑149 (MQ=255)
                     aCCAGCGGCGCATTGCCCGCCGCTTCGGCCAGCTTGAGGTGGAACTCACCGGAAAGGCGGATACCGGCACCCCGGTCACCGCACGAGAAGCTG‑TCGCGCTCTTCGCGCACCATCTGCCGCAGCTCGTTGA‑GCTGCTCGGGGGTGGCAtg                                                                                                                               >  8:135266/1‑149 (MQ=255)
                      ccAGCGGCGCATTGCCCGCCGCTTCGGCCAGCTTGAGGTGGAACTCACCGGAAAGGCGGATACCGGCACCCCGGTCACCGCACGAGAAGCTG‑TCGCGCTCTTCGCGCACCATCTGCCGCAGCTCGTTGA‑GCTGCTCGGGGGTGGCAtgc                                                                                                                              >  5:182950/1‑149 (MQ=255)
                                              cGGCCAGCTTGAGGTGGAACTCACCGGAAAGGCGGATACCGGCACCCCGGTCACCGCACGAGAAGCTG‑TCGCGCTCTTCGCGCACCATCTGCCGCAGCTCGTTGA‑GCTGCTCGGGGGTGGCATGCTGCACCGCCAGCTCGGTGATGgc                                                                                                       <  4:166402/148‑1 (MQ=255)
                                              cGGCCAGCTTGAGGTGGAACTCACCGGAAAGGCGGATACCGGCACCCCGGTCACCGCACGAGAAGCTG‑TCGCGCTCTTCGCGCACCATCTGCCGCAGCTCGTTGA‑GCTGCTCGGGGGTGGCATGCTGCACCGCCAGCTCGGTGATGgc                                                                                                       <  5:203527/148‑1 (MQ=255)
                                                 ccAGCTTGAGGTGGAACTCACCGGAAAGGCGGATACCGGCACCCCGGTCACCGCACGAGAAGCTG‑TCGCGCTCTTCGCGCACCATCTGCCGCAGCTCGtt                                                                                                                                                     <  5:229916/100‑1 (MQ=255)
                                                 ccAGCTTGAGGTGGAACTCACCGGAAAGGCGGATACCGGCACCCCGGTCACCGCACGAGAAGCTG‑TCGCGCTCTTCGCGCACCATCTGCCGCAGCTCGtt                                                                                                                                                     >  6:229916/1‑100 (MQ=255)
                                                     cTTGAGGTGGAACTCACCGGAAAGGCGGATACCGGCACCCCGGTCACCGCACGAGAAGCTG‑TCGCGCTCTTCGCGCACCATCTGCCGCAGCTCGTTGATGCTGCTCGGGGGTGGCATGCTGCACCGCCAGCTCg                                                                                                               <  1:243323/134‑1 (MQ=255)
                                                     cTTGAGGTGGAACTCACCGGAAAGGCGGATACCGGCACCCCGGTCACCGCACGAGAAGCTG‑TCGCGCTCTTCGCGCACCATCTGCCGCAGCTCGTTGA‑GCTGCTCGGGGGTGGCATGCTGCACCGCCAGCTCg                                                                                                               >  2:243323/1‑133 (MQ=255)
                                                         aGGTGGAACTCACCGGAAAGGCGGATACCGGCACCCCGGTCACCGCACGAGAAGCTG‑TCGCGCTCTTCGCGCACCATCTGCCGCAGCTCGTTGA‑GCTGCTCGGGGGTGGCATGCTGCACCGCCAGCTCGGTGATGGCGCGTTCGACCa                                                                                            <  7:135266/148‑1 (MQ=255)
                                                          ggTGGAACTCACCGGAAAGGCGGATACCGGCACCCCGGTCACCGCACGAGAAGCTG‑TCGCGCTCTTCGCGCACCATCTGCCGCAGCTCGTTGA‑GCTGCTCGGGGGTGGCATGCTGCACCGCCAGCTCGGTGATGGCGCGTTCGACCATg                                                                                          <  5:370673/149‑1 (MQ=255)
                                                           gTGGAACTCACCGGAAAGGCGGATACCGGCACCCCGGTCACCGCACGAGAAGCTG‑TCGCGCTCTTCGCGCACCATCTGCCGCAGCTCGTTGA‑GCTGCTCGGGGGTGGCATGCTGCACCGCCAGCTCGGTGATGGCGCGTTCGACCATgc                                                                                         <  6:182950/149‑1 (MQ=255)
                                                              gAACTCACCGGAAAGGCGGATACCGGCACCCCGGTCACCGCACGAGAAGCTG‑TCGCGCTCTTCGCGCACCATCTGCCGCAGCTCGTTGA‑GCTGCTCGGGGGTGGCATGCTGCACCGCCAGCTCGGTGATGGCGCGTTCGACCATGcgcc                                                                                      >  4:318172/1‑149 (MQ=255)
                                                                aCTCACCGGAAAGGCGGATACCGGCACCCCGGTCACCGCACGAGAAGCTG‑TCGCGCTCTTCGCGCACCATCTGCCGCAGCTCGTTGA‑GCTGCTCGGGGGTGGCATGCTGCAc                                                                                                                         >  6:59404/1‑112 (MQ=255)
                                                                aCTCACCGGAAAGGCGGATACCGGCACCCCGGTCACCGCACGAGAAGCTG‑TCGCGCTCTTCGCGCACCATCTGCCGCAGCTCGTTGA‑GCTGCTCGGGGGTGGCATGCTGCAc                                                                                                                         <  5:59404/112‑1 (MQ=255)
                                                                aCTCACCGGAAAGGCGGATACCGGCACCCCGGTCACCGCACGAGAAGCTG‑TCGCGCTCTTCGCGCACCATCTGCCGCAGCTCGTTGA‑GCTGCTCGGGGGTGGCATGCTGCACCGCCAGCTCGGTGATGGCGCGTTCGACCATGcgccgc                                                                                    <  1:280333/149‑1 (MQ=255)
                                                                    aCCGGAAAGGCGGATACCGGCACCCCGGTCACCGCACGAGAAGCTG‑TCGCGCTCTTCGCGCACCATCTGCCGCAGCTCGTTGA‑GCTGCTCGGGGGTGGCATGCTGCACCGCCAGCTCGGTGATGGCGCGTTCGACCATGCGCCGCgag                                                                                 >  6:90250/1‑148 (MQ=255)
                                                                    aCCGGAAAGGCGGATACCGGCACCCCGGTCACCGCACGAGAAGCTG‑TCGCGCTCTTCGCGCACCATCTGCCGCAGCTCGTTGA‑GCTGCTCGGGGGTGGCATGCTGCACCGCCAGCTCGGTGATGGCGCGTTCGACCATGCGCCGCgag                                                                                 >  1:152448/1‑148 (MQ=255)
                                                                        gAAAGGCGGATACCGGCACCCCGGTCACCGCACGAGAAGCTG‑TCGCGCTCTTCGCGCACCATCTGCCGCAGCTCGTTGA‑GCTGCTCGGGGGTGGCATGCTGCACCGCCAGCTCGGTGATGGCGCGTTCGAc                                                                                              >  7:350851/1‑131 (MQ=255)
                                                                        gAAAGGCGGATACCGGCACCCCGGTCACCGCACGAGAAGCTG‑TCGCGCTCTTCGCGCACCATCTGCCGCAGCTCGTTGA‑GCTGCTCGGGGGTGGCATGCTGCACCGCCAGCTCGGTGATGGCGCGTTCGAc                                                                                              <  8:350851/131‑1 (MQ=255)
                                                                        gAAAGGCGGATACCGGCACCCCGGTCACCGCACGAGAAGCTG‑TCGCGCTCTTCGCGCACCATCTGCCGCAGCTCGTTGA‑GCTGCTCGGGGGTGGCATGCTGCACCGCCAGCTCGGTGATGGCGCGTTCGACCATGCGCCGCGAgaagaa                                                                            >  7:150536/1‑149 (MQ=255)
                                                                                         aCCCCGGTCACCGCACGAGAAGCTG‑TCGCGCTCTTCGCGCACCATCTGCCGCAGCTCGTTGA‑GCTGCTCGGGGGTGGCATGCTGCACCGCCAGCTCGGTGATGGCGCGTTCGACCATGCGCCGCGAGAAGAACACCTGGCGCGCCTCtt                                                           <  8:150536/149‑1 (MQ=255)
                                                                                          ccccGGTCACCGCACGAGAAGCTG‑TCGCGCTCTTCGCGCACCATCTGCCGCAGCTCGTTGA‑GCTGCTCGGGGGTGGCATGCTGCACCGCCAGCTCGGTGATGGCGCGTTCGACCATGCGCCGCGAGAAGAACACCTGGCGCGCCTCTTc                                                          <  7:78566/149‑1 (MQ=255)
                                                                                           cccGGTCACCGCACGAGAAGCTG‑TCGCGCTCTTCGCGCACCATCTGCCGCAGCTCGTTGA‑GCTGCTCGGGGGTGGCATGCTGCACCGCCAGCTCGGTGATGGCGCGTTCGACCATGCGCCGCGAGAAGAACACCTGGCGCGCCTCTTc                                                          >  6:378045/1‑148 (MQ=255)
                                                                                                       aCGAGAAGCTG‑TCGCGCTCTTCGCGCACCATCTGCCGCAGCTCGTTGA‑GCTGCTCGGGGGTGGCATGCTGCACCGCCAGCTCGGTGATGGCGCGTTCGACCATGCGCCGCGAGAAGAACACCTGGCGCGCCTCTTCGACGGTCGGGCTg                                             >  1:131649/1‑149 (MQ=255)
                                                                                                         gagaAGCTG‑TCGCGCTCTTCGCGCACCATCTGCCGCAGCTCGTTGA‑GCTGCTCGGGGGTGGCATGCTGCACCGCCAGCTCGGTGATGGCGCGTTCGACCATGCGCCGCGAGAAGAACACCTGGCGCGCCTCTTCGACGGTCGGGCTGGc                                           <  5:90250/149‑1 (MQ=255)
                                                                                                                tG‑TCGCGCTCTTCGCGCACCATCTGCCGCAGCTCGTTGA‑GCTGCTCGGGGGTGGCATGCTGCACCGCCAGCTCGGTGATGGCGCGTTCGACCATGCGCCGCGAGAAGAACACCTGGCGCGCCTCTTCGACGGTCGGGCTGGCCaccacc                                    >  5:293075/1‑149 (MQ=255)
                                                                                                                tG‑TCGCGCTCTTCGCGCACCATCTGCCGCAGCTCGTTGA‑GCTGCTCGGGGGTGGCATGCTGCACCGCCAGCTCGGTGATGGCGCGTTCGACCATGCGCCGCGAGAAGAACACCTGGCGCGCCTCTTCGACGGTCGGGCTGGCCaccacc                                    >  8:147517/1‑149 (MQ=255)
                                                                                                                   tCGCGCTCTTCGCGCACCATCTGCCGCAGCTCGTTGA‑GCTGCTCGGGGGTGGCATGCTGCCCCGCCAGCTCGGTGATGGCGCGTTCGACCATGCGCCGCGAGAAGAACACCTGGCGCGCCTCTTCGACGGTCGGGCTGGCCACcaccgc                                  >  7:250024/1‑149 (MQ=255)
                                                                                                                   tCGCGCTCTTCGCGCACCATCTGCCGCAGCTCGTTGA‑GCTGCTCGGGGGTGGCATGCTGCACCGCCAGCTCGGTGATGGCGCGTTCGACCATGCGCCGCGAGAAGAACACCTGGCGCGCCTCTTCGACGGTCGGGCTGGCCACcaccg                                   >  1:337694/1‑148 (MQ=255)
                                                                                                                   tCGCGCTCTTCGCGCACCATCTGCCGCAGCTCGTTGA‑GCTGCTCGGGGGTGGCATGCTGCACCGCCAGCTCGGTGATGGCGCGTTCGACCATGCGCCGCGAGAAGAACACCTGGCGCGCCTCTTCGACGGTCGGGCTGGCCACcaccg                                   >  7:136913/1‑148 (MQ=255)
                                                                                                                     gcgcTCTTCGCGCACCATCTGCCGCAGCTCGTTGA‑GCTGCTCGGGGGTGGCATGCTGCACCGCCAGCTCGGTGATGGCGCGTTCGACCATGCGCCGCGAGAAGAACACCTGGCGCGCCTCTTCGACGGTCGGGCTGGCCACcaccgcac                                >  3:257228/1‑149 (MQ=255)
                                                                                                                     gcgcTCTTCGCGCACCATCTGCCGCAGCTCGTTGA‑GCTGCTCGGGGGTGGCATGCTGCACCGCCAGCTCGGTGATGGCGCGTTCGACCATGCGCCGCGAGAAGAACACCTGGCGCGCCTCTTCGACGGTCGGGCTGGCCACcaccgcac                                >  2:129855/1‑149 (MQ=255)
                                                                                                                     gcgcTCTTCGCGCACCATCTGCCGCAGCTCGTTGA‑GCTGCTCGGGGGTGGCATGCTGCACCGCCAGCTCGGTGATGGCGCGTTCGACCATGCGCCGCGAGAAGAACACCTGGCGCGCCTCTTCGACGGTCGGGCTGGCCACcaccgca                                 <  5:378045/148‑1 (MQ=255)
                                                                                                                        ctctTCGCGCACCATCTGCCGCAGCTCGTTGA‑GCTGCTCGGGGGTGGCATGCTGCACCGCCAGCTCGGTGATGGCGCGTTCGACCATGCGCCGCGAGAAGAACACCTGGCGCGCCTCTTCGACGGTCGGGCTGGCCACCaccgcaccgc                             <  2:337694/149‑1 (MQ=255)
                                                                                                                             cgcgCACCATCTGCCGCAGCTCGTTGA‑GCTGCTCGGGGGTGGCATGCTGCACCGCCAGCTCGGTGATGGCGCGTTCGACCATGCGCCGCGAGAAGAACACCTGGCGCGCCTCTTCGACGGTCGGGCTGGCCACCACCGCACCGCGGtt                         <  5:67268/148‑1 (MQ=255)
                                                                                                                                  aCCATCTGCCGCAGCTCGTTGA‑GCTGCTCGGGGGTGGCATGCTGCACCGCCAGCTCGGAGATGGCGCGTTCGACCATGCGCCGCGAGAAGAACACCTGGCGCGCCTCTTCGACGGTCGGGCTGGCCACCACCGCACCGCGGTTCGGTCg                   >  5:266473/1‑149 (MQ=255)
                                                                                                                                     aTCTGCCGCAGCTCGTTGA‑GCTGCTCGGGGGTGGCATGCTGCACCGCCAGCTCGGTGATGGCGCGTTCGACCATGCGCCGCGAGAAGAACACCTGGCGCGCCTCTTCGACTGGCGGGCTGGCCACCACCGCACCGCGGTTCGGTCgcag                <  2:326665/149‑1 (MQ=255)
                                                                                                                                      ccccgagCAGCTCAACGA‑GCTGCTCGGGGGTGGCATGCTGCACCGCCAGCTCGGTGATGGCGCGTTCGACCATGCGCCGCGAGAAGAACACCTGGCGCGCCTCTTCGACGGTCGGGCTGGCCACCACCGCACCGCGGTTCGGTCgc                  >  4:358353/7‑146 (MQ=255)
                                                                                                                                      ccccgagCAGCTCAACGA‑GCTGCTCGGGGGTGGCATGCTGCACCGCCAGCTCGGTGATGGCGCGTTCGACCATGCGCCGCGAGAAGAACACCTGGCGCGCCTCTTCGACGGTCGGGCTGGCCACCACCGCACCGCGGTTCGGTCgc                  <  3:358353/140‑1 (MQ=255)
                                                                                                                                        ccgagCAGCTCAACGA‑GCTGCTCGGGGGTGGCATGCTGCACCGCCAGCTCGGTGATGGCGCGTTCGACCATGCGCCGCGAGAAGAACACCTGGCGCGCCTCTTCGACGGTCGGGCTGGCCACCACCGCACCGCGGTTCGGTCgcagcag             >  4:41713/5‑149 (MQ=255)
                                                                                                                                          gagCAGCTCAACGA‑GCTGCTCGGGGGTGGCATGCTGCACCGCCAGCTCGGTGATGGCGCGTTCGACCATGCGCCGCGAGAAGAACACCTGGCGCGCCTCTTCGACGGTCGGGCTGGCCACCACCGCACCGCGGTTCGGTCgcagcagca           >  7:328848/3‑149 (MQ=255)
                                                                                                                                          gagCAGCTCAACGA‑GCTGCTCGGGGGTGGCATGCTGCACCGCCAGCTCGGTGATGGCGCGTTCGACCATGCGCCGCGAGAAGAACACCTGGCGCGCCTCTTCGACGGTCGGGCTGGCCACCACCGCACCGCGGTTCGGTCgcagcagca           >  5:225554/3‑149 (MQ=255)
                                                                                                                                          ccGCAGCTCGTTGA‑GCTGCTCGGGGGTGGCATGCTGCACCGCCAGCTCGGTGATGGCGCGGTCGACCATGCGCCGCGAGAAGAACACCTGGCGCGCCTCTTCGACGGTCGGGCTGGCCACCACCGCACCGCGGTTCGGTCgcagca              <  1:295095/146‑1 (MQ=255)
                                                                                                                                                   gTTGA‑GCTGCTCGGGGGTGGCATGCTGCACCGCCAGCTCGGTGATGGCGCGTTCGACCATGCGCCGCGAGAAGAACACCTGGCGCGCCTCTTCGACGGTCGGGCTGGCCACCACCGCACCGCGGTTCGGTCGCAGCAGCACCACGCttt  >  8:108655/1‑149 (MQ=255)
                                                                                                                                                   gTTGA‑GCTGCTCGGGGGTGGCATGCTGCACCGCCAGCTCGGTGATGGCGCGTTCGACCATGCGCCGCGAGAAGAACACCTGGCGCGCCTCTTCGACGGTCGGGCTGGCCACCACCGCACCGCGGTTCGGTCGCAGCAGCACCACGCttt  >  2:57597/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                                     
ACCAGGCTGCGCTGGAAACTCACCAGCGGCGCATTGCCCGCCGCTTCGGCCAGCTTGAGGTGGAACTCACCGGAAAGGCGGATACCGGCACCCCGGTCACCGCACGAGAAGCTG‑TCGCGCTCTTCGCGCACCATCTGCCGCAGCTCGTTGA‑GCTGCTCGGGGGTGGCATGCTGCACCGCCAGCTCGGTGATGGCGCGTTCGACCATGCGCCGCGAGAAGAACACCTGGCGCGCCTCTTCGACGGTCGGGCTGGCCACCACCGCACCGCGGTTCGGTCGCAGCAGCACCACGCTTT  >  NC_002947/4874024‑4874318

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑