Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	4,905,502	A→G	7.0%	intergenic (‑96/+32)	PP_4313 ← / ← PP_4314	peptidylprolyl isomerase/hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	4,905,502	0	A	G	7.0%	250.5 / 6.7	85	intergenic (‑96/+32)	PP_4313/PP_4314	peptidylprolyl isomerase/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (41/38);  new base G (3/3);  total (44/41)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.38e-01

TTTGCCGTCTCCCTCGACCAGGTCGATGATCTGCAGTTCTTGGCTCACGGGTACACTCCAAACGCTGAAAAACAAAACCGCCCTTTTCTCAGGAATGCACCGGTTTGGCAAGTTCACGCAGAATTGTACGGAGGCATT‑CGCCGACGGA‑ATGCCTCCGTACAGACATGACGTTGAATCAGCTGTTCTGCTCGAA‑‑CACCTCTTGGCCGGACAGCTCCTTGGTCTGATTGGCGAAGCAGTACCACGCGGGTTTCTCTTCGACGAAGATCTGCAGGTCGAAATCCCAGGCTTGGT  >  NC_002947/4905359‑4905649                                                                                                                                                 |                                                                                                                                                       tttGCCGTCTCCCTCGACCAGGTCGATGATCTGCAGTTCTTGGCTCACGGGTACACTCCAAACGCTGAAAAACAAAACCGCCCTTTTCTCAGGAATGCACCGGTTTGGCAAGTTCACGCAGAATTGTACGGAGGCATT‑CGCCGACGGa                                                                                                                                                    >  5:23307/1‑148 (MQ=255)   tGCCGTCTCCCTCGACCAGGTCGATGATCTGCAGTTCTTGGCTCACGGGTACACTCCAAACGCTGAAAAACAAAACCGCCCTTTTCTCAGGAATGCACCGGTTTGGCAAGTTCACGCAGAATTGTACGGAGGCATT‑CGCCGACGGa                                                                                                                                                    <  6:23307/146‑1 (MQ=255)   tGCCGTCTCCCTCGACCAGGTCGATGATCTGCAGTTCTTGGCTCACGGGTACACTCCAAACGCTGAAAAACAAAACCGCCCTTTTCTCAGGAATGCACCGGTTTGGCAAGTTCACGCAGAATTGTACGGAGGCATT‑CGCCGACGGA‑At                                                                                                                                                 <  8:215044/148‑1 (MQ=255)     ccGTCTCCCTCGACCAGGTCGATGATCTGCAGTTCTTGGCTCACGGGTACACTCCAAACGCTGAAAAACAAAACCGCCCTTTTCTCAGGAATGCACCGGTTTGGCAAGTTCACGCAGAATTGTACGGAGGCATT‑CGCCGACGGA‑ATGc                                                                                                                                               <  7:102772/148‑1 (MQ=255)          tcCCTCGACCAGGTCGATGATCTGCAGTTCTTGGCTCATGGGTACACTCCAAACGCTGAAAAACAAAACCGCCCTTTTCTCAGGAATGCACCGGTTTGGCAAGTTCACGCAGAATTGTACGGAGGCATT‑CGCCGACGGA‑ATGCCTCCg                                                                                                                                          >  4:363013/1‑148 (MQ=255)                gACCAGGTCGATGATCTGCAGTTCTTGGCTCATGGGTACACTCCAAACGCTGAAAAACAAAACCGCCCTTTTCTCAGGAATGCACCGGTTTGGCAAGTTCACGCAGAATTGTACGGAGGCATT‑CGCCGACGGA‑ATGCCTCCGTACAg                                                                                                                                     <  3:363013/147‑1 (MQ=255)                gACCAGGTCGATGATCTGCAGTTCTTGGCTCACGGGTACACTCCAAACGCTGAAAAACAAAACCGCCCTTTTCTCAGGAATGCACCGGTTTGGCAAGTTCACGCAGAATTGTACGGAGGCATT‑CGCCGACGGA‑ATGCCTCCGTACAGa                                                                                                                                    <  6:68538/148‑1 (MQ=255)                   cAGGTCGATGATCTGCAGTTCTTGGCTCACGGGTACACTCCAAACGCTGAAAAACAAAACCGCCCTTTTCTCAGGAATGCACCGGTTTGGCAAGTTCACGCAGAATTGTACGGAGGCATT‑CGCCGACGGA‑ATGCCTCCGTACAGACAt                                                                                                                                 >  2:65766/1‑148 (MQ=255)                         gatgatCTGCAGTTCTTGGCTCACGGGTACACTCCAAACGCTGAAAAACAAAACCGCCCTTTTCTCAGGAATGCACCGGTTTGGCAAGTTCACGCAGAATTGTACGGAGGCATT‑CGCCGACGGA‑ATGCCTCCGTACAGACATGACGtt                                                                                                                           <  7:162892/148‑1 (MQ=255)                              tCTGCAGTTCTTGGCTCACGGGTACACTCCAAACGCTGAAAAACAAAACCGCCCTTTTCTCAGGAATGCACCGGTTTGGCAAGTTCACGCAGAATTGTACGGAGGCATT‑CGCCGACGGA‑ATGCCTCCGTACAGACATGACGTTGAAt                                                                                                                       >  7:128656/1‑147 (MQ=255)                              tCTGCAGTTCTTGGCTCACGGGTACACTCCAAACGCTGAAAAACAAAACCGCCCTTTTCTCAGGAATGCACCGGTTTGGCAAGTTCACGCAGAATTGTACGGAGGCATT‑CGCCGACGGA‑ATGCCTCCGTACAGACATGACGTTGAAt                                                                                                                       >  5:98369/1‑147 (MQ=255)                              tCTGCAGTTCTTGGCTCACGGGTACACTCCAAACGCTGAAAAACAAAACCGCCCTTTTCTCAGGAATGCACCGGTTTGGCAAGTTCACGCAGAATTGTACGGAGGCATT‑CGCCGACGGA‑ATGCCTCAGTACAGACATGACGTTGAATc                                                                                                                      >  1:166167/1‑148 (MQ=255)                                 gCAGTTCTTGGCTCACGGGTACACTCCAAACGCTGAAAAACAAAACCGCCCTTTTCTCAGGAATGCACCGGTTTGGCAAGTTCACGCAGAATTGTACGGAGGCATTCCGTCGGC‑GA‑ATGCCTcc                                                                                                                                           >  4:52222/1‑124 (MQ=38)                                 gCAGTTCTTGGCTCACGGGTACACTCCAAACGCTGAAAAACAAAACCGCCCTTTTCTCAGGAATGCACCGGTTTGGCAAGTTCACGCAGAATTGTACGGAGGCATTCCGTCGGC‑GA‑ATGCCTcc                                                                                                                                           <  3:52222/124‑1 (MQ=38)                                 gCAGTTCTTGGCTCACGGGTACACTCCAAACGCTGAAAAACAAAACCGCCCTTTTCTCAGGAATGCACCGGTTTGGCAAGTTCACGCAGAATTGTACGGAGGCATT‑CGCCGACGGA‑ATGCCTCCGTACAGACATGACGTTGAATCAGCt                                                                                                                  >  6:59446/1‑149 (MQ=255)                                     ttCTTGGCTCACGGGTACACTCCAAACGCTGAAAAACAAAACCGCCCTTTTCTCAGGAATGCACCGGTTTGGCAAGTTCACGCAGAATTGTACGGAGGCATT‑CGCCGACGGA‑ATGCCTCCGTACa                                                                                                                                      >  5:250383/1‑125 (MQ=255)                                     ttCTTGGCTCACGGGTACACTCCAAACGCTGAAAAACAAAACCGCCCTTTTCTCAGGAATGCACCGGTTTGGCAAGTTCACGCAGAATTGTACGGAGGCATT‑CGCCGACGGA‑ATGCCTCCGTACa                                                                                                                                      <  6:250383/125‑1 (MQ=255)                                       cTTGGCTCACGGGTACACTCCAAACGCTGAAAAACAAAACCGCCCTTTTCTCAGGAATGCACCGGTTTGGCAAGTTCACGCAGAATTGTACGGAGGCATT‑CGCCGACGGACATGCCTCGGTACAGACATGACGTTGAATCAGCTGTTc                                                                                                              <  1:65766/148‑1 (MQ=255)                                        ttGGCTCACGGGTACACTCCAAACGCTGAAAAACAAAACCGCCCTTTTCTCAGGAATGCACCGGTTTGGCAAGTTCACGCAGAATTGTACGGAGGCATT‑CGCCGACGGA‑ATGCCTCCGTACAGACATGACGTTGAATCAGCTGTTCTGc                                                                                                           >  2:30399/1‑149 (MQ=255)                                        ttGGCTCACGGGTACACTCCAAACGCTGAAAAACAAAACCGCCCTTTTCTCAGGAATGCACCGGTTTGGCAAGTTCACGCAGAATTGTACGGAGGCATT‑CGCCGACGGA‑ATGCCTCCGTACAGACATGACGTTGAATCAGCTGTTCTGc                                                                                                           >  3:269941/1‑149 (MQ=255)                                             tCACGGGTACACTCCAAACGCTGAAAAACAAAACCGCCCTTTTCTCAGGAATGCACCGGTTTGGCAAGTTCACGCAGAATTGTACGGAGGCATT‑CGCCGACGGA‑ATGCCTCCGTACAGACATGACGTTGAATCAGCTGTTCTGCTCGaa                                                                                                      >  5:67581/1‑149 (MQ=255)                                             tCACGGGTACACTCCAAACGCTGAAAAACAAAACCGCCCTTTTCTCAGGAATGCACCGGTTTGGCAAGTTCACGCAGAATTGTACGGAGGCATT‑CGCCGACGGA‑ATACCTCCGTACAGACATGACGTTGAATCAg                                                                                                                    >  6:325291/1‑135 (MQ=255)                                             tCACGGGTACACTCCAAACGCTGAAAAACAAAACCGCCCTTTTCTCAGGAATGCACCGGTTTGGCAAGTTCACGCAGAATTGTACGGAGGCATT‑CGCCGACGGA‑ATACCTCCGTACAGACATGACGTTGAATCAg                                                                                                                    <  5:325291/135‑1 (MQ=255)                                               aCGGGTACACTCCAAACGCTGAAAAACAAAACCGCCCTTTTCTCAGGAATGCACCGGTTTGGCAAGTTCACGCAGAATTGTACGGAGGCATT‑CGCCGACGTA‑ATGCCTCCGTACAGACATGACGTTGAATCAGCTGTTCTGCTCGaa                                                                                                      <  5:192693/147‑1 (MQ=255)                                                      cacTCCAAACGCTGAAAAACAAAACCGCCCTTTTCTCAGGAATGCACCGGTTTGGCAAGTTCACGCAGAATTGTACGGAGGCATT‑CGCCGACGGA‑ATGCCTCCGTACAGACATGACGTTGAATCAGCTGTTCTGCTCGAA‑‑CACCTCtt                                                                                            <  4:269941/148‑1 (MQ=255)                                                       acTCCAAACGCTGAAAAACAAAACCGCCCTTTTCTCAGGAATGCACCGGTTTGGCAAGTTCACGCAGAATTGTACGGAGGCATT‑CGCCGACGGA‑ATGCCTCCGTACAGACATGACGTTGAATCAGCTGTTCTGCTCGAA‑‑CACCTCTTgg                                                                                          >  6:307330/1‑149 (MQ=255)                                                         tCCAAACGCTGAAAAACAAAACCGCCCTTTTCTCAGGAATGCACCGGTTTGGCAAGTTCACGCAGAATTGTACGGAGGCATT‑CGCCGACGGA‑ATGCCTCCGTACAGACATGACGTTGAATCAGCTGTTCTGCTCGAA‑‑CACCTCTTGGcc                                                                                        >  6:310362/1‑149 (MQ=255)                                                         tCCAAACGCTGAAAAACAAAACCGCCCTTTTCTCAGGAATGCACCGGTTTGGCAAGTTCACGCAGAATTGTACGGAGGCATT‑CGCCGACGGA‑ATGCCTCCGTACAGACATGACGTTGAATCAGCTGTTCTGCTCGAA‑‑CACCTCTTGGcc                                                                                        >  1:226469/1‑149 (MQ=255)                                                          ccAAACGCTGAAAAACAAAACCGCCCTTTTCTCAGGAATGCACCGGTTTGGCAAGTTCACGCAGAATTGTACGGAGGCATT‑CGCCGACGGA‑ATGCCTCCGTACAGACATGACGTTGAATCAGCTGTTCTGCTCGAA‑‑CACCTCTTGGCCg                                                                                       >  4:105075/1‑149 (MQ=255)                                                             aaCGCTGAAAAACAAAACCGCCCTTTTCTCAGGAATGCACCGGTTTGGCAAGTTCACGCAGAATTGTACGGAGGCATT‑CGCCGACGGA‑ATGCCTCCGTACAGACATGACGTTGAATCAGCTGTTCTGCTCGAA‑‑CACCTCTTGGCCGGAc                                                                                    <  4:71874/149‑1 (MQ=255)                                                                   gAAAAACAAAACCGCCCTTTTCTCAGGAATGCACCGGTTTGGCAAGTTCACGCAGAATTGTACGGAGGCATT‑CGCCGACGGA‑ATGCCTCCGTACAGACATGACGTTGAATCAGCTGTTCTGCTc                                                                                                         <  2:284680/124‑1 (MQ=255)                                                                   gAAAAACAAAACCGCCCTTTTCTCAGGAATGCACCGGTTTGGCAAGTTCACGCAGAATTGTACGGAGGCATT‑CGCCGACGGA‑ATGCCTCCGTACAGACATGACGTTGAATCAGCTGTTCTGCTc                                                                                                         >  1:284680/1‑124 (MQ=255)                                                                    aaaaaCAAAACCGCCCTTTTCTCAGGAATGCACCGGTTTGGCAAGTTCACGCAGAATTGTACGGAGGCATT‑CGCCGACGGA‑ATGCCTCCGTACAGACATGACGTTGAATCAGCTGTTCTGCTCGAA‑‑CACCTCTTGGCCGGACAGCTCCt                                                                             <  5:59446/149‑1 (MQ=255)                                                                    aaaaaCAAAACCGCCCTTTTCTCAGGAATGCACCGGTTTGGCAAGTTCACGCAGAATTGTACGGAGGCATT‑CGCCGACGGA‑ATGCCTCCGTACAGACATGACGTTGAATCAGCTGTTCTGCTCGAA‑‑CACCTCTTGGCCGGACAGCTCCt                                                                             <  1:229084/149‑1 (MQ=255)                                                                      aaacaaaacCGCCCTTTTCTCAGGAATGCACCGGTTTGGCAAGTTCACGCAGAATTGTACGGAGGCATT‑CGCCGACGGA‑ATGCCTCCGTACAGACATGACGTTGAATCAGCTGTTCTGCTCGAA‑‑CACCTCTTGGCCGGACAGCTCCTTg                                                                           >  2:237094/1‑149 (MQ=255)                                                                      aaacaaaacCGCCCTTTTCTCAGGAATGCACCGGTTTGGCAAGTTCACGCAGAATTGTACGGAGGCATT‑CGCCGACGGA‑ATGCCTCCGTACAGACATGACGTTGAATCAGCTGTTCTGCTCGAA‑‑CACCTCTTGGCCGGACAGCTCCTTg                                                                           <  6:67581/149‑1 (MQ=255)                                                                         caaaacCGCCCTTTTCTCAGGAATGCACCGGTTTGGCAAGTTCACGCAGAATTGTACGGAGGCATT‑CGCCGACGGA‑ATGCCTCCGTACAGACATGACGTTGAATCAGCTGTTCTGCTCGAA‑‑CACCTCTTGGCCGGACAGCTCCTTg                                                                           <  1:108502/146‑1 (MQ=255)                                                                         caaaacCGCCCTTTTCTCAGGAATGCACCGGTTTGGCAAGTTCACGCAGAATTGTACGGAGGCATT‑CGCCGACGGA‑ATGCCTCCGTACAGACATGACGTTGAATCAGCTGTTCTGCTCGAA‑‑CACCTCTTGGCCGGACAGCTCCTTg                                                                           >  2:108502/1‑146 (MQ=255)                                                                                gCCCTTTTCTCAGGAATGGACCGGTTTGGCAAGTTCACGCAGAATTGTACGGAGGCATT‑CGCCGACGGA‑ATGCCTCCGTACAGACATGACGTTGAATCAGCTGTTCTGCTCGAA‑‑CACCTCTTGGCCGGACAGCTCCTTGGTCTGATTgg                                                                 >  1:18158/1‑149 (MQ=255)                                                                                 cccTTTTCTCAGGAATGCACCGGTTTGGCAAGTTCACGCAGAATTGTACGGAGGCATT‑CGCCGACGGA‑ATGCCTCCGTACAGACATGACGTTGAATCAGCTGTTCTGCTCGAA‑‑CACCTCTTGGCCGGACAGCTCCTTGGTCTGATTGGc                                                                <  5:307330/149‑1 (MQ=255)                                                                                  ccTTTTCTCAGGAATGCACCGGTTTGGCAAGTTCACGCAGAATTGTACGGAGGCATT‑CGCCGACGGA‑ATGCCTCCGTACAGACATGACGTTGAATCAGCTGTTCTGCTCGAA‑‑CACCTCTTGGCCg                                                                                       >  1:112581/1‑125 (MQ=255)                                                                                  ccTTTTCTCAGGAATGCACCGGTTTGGCAAGTTCACGCAGAATTGTACGGAGGCATT‑CGCCGACGGA‑ATGCCTCCGTACAGACATGACGTTGAATCAGCTGTTCTGCTCGAA‑‑CACCTCTTGGCCg                                                                                       <  2:112581/125‑1 (MQ=255)                                                                                  ccTTTTCTCAGGAATGCACCGGTTTGGCAAGTTCACGCAGAATTGTACGGAGGCATT‑CGCCGACGGA‑ATGCCTCCGTACAGACATGACGTTGAATCAGCTGTTCTGCTCGAA‑‑CACCTCTTGGCCGGACAGCTCCTTGGTCTGATTGGc                                                                >  5:187664/1‑148 (MQ=255)                                                                                    ttttCTCAGGAATGCACCGGTTTGGCAAGTTCACGCAGAATTGTACGGAGGCATT‑CGCCGACGGA‑ATGCCTCCGTACAGACATGACGTTGAATCAGCTGTTCTGCTCGAA‑‑CACCTCTTGGCCGGACAGCTCCTTGGTc                                                                        >  4:156993/1‑138 (MQ=255)                                                                                    ttttCTCAGGAATGCACCGGTTTGGCAAGTTCACGCAGAATTGTACGGAGGCATT‑CGCCGACGGA‑ATGCCTCCGTACAGACATGACGTTGAATCAGCTGTTCTGCTCGAA‑‑CACCTCTTGGCCGGACAGCTCCTTGGTc                                                                        <  3:156993/138‑1 (MQ=255)                                                                                      ttCTCAGGAATGCACCGGTTTGGCAAGTTCACGCAGAATTGTACGGAGGCATT‑CGCCGACGGA‑ATGCCTCCGTACAGACATGACGTTGAATCAGCTGTTCTGCTCGAA‑‑CACCTCTTGGCCGGACAGCTCCTTGGTCTGATTGGCGAAg                                                            <  6:187664/148‑1 (MQ=255)                                                                                       tctcAGGAATGCATCGGTTTGGCAAGTTCACGCAGAATTGTACGGAGGCATT‑CGCCGACGGA‑ATGCCTCCGTACAGACATGACGTTGAATCAGCTGTTCTGCTCGAA‑‑CACCTCTTGGCCGGACAGCTCCTTGGTCTGATTGGCGAAg                                                            <  7:23062/147‑1 (MQ=255)                                                                                       tctcAGGAATGCATCGGTTTGGCAAGTTCACGCAGAATTGTACGGAGGCATT‑CGCCGACGGA‑ATGCCTCCGTACAGACATGACGTTGAATCAGCTGTTCTGCTCGAA‑‑CACCTCTTGGCCGGACAGCTCCTTGGTCTGATTGGCGAAg                                                            >  8:23062/1‑147 (MQ=255)                                                                                       tctcAGGAATGCACCGGTTTGGCAAGTTCACGCAGAATTGTACGGAGGCATT‑CGCCGACGGA‑ATGCCTCCGTACAGACATGACGTTGAATCAGCTGTTCTGCTCGAA‑‑CACCTCTTGGCCGGACAGCTCCTTGGTCTGATTGGCGAAGc                                                           <  6:305138/148‑1 (MQ=255)                                                                                         tcAGGAATGGAGCGGTTTGGCAAGTTCACGCATAATTGTACGGAGGCATT‑CGCCGACGGA‑ATGCCTCCGTACAGACATGACGTTGAATCAGCTGTTCTGCTCGAA‑‑CACCTCTTGGCCGGACAGCTCCTTGGTCTGATTGGCGAAGCAGt                                                        <  2:166167/149‑1 (MQ=255)                                                                                         tcAGGAATGCACCGGTTTGGCAAGTTCACGCAGAATTGTACGGAGGCATT‑CGCCGACGGA‑ATGCCTCCGTACAGACAt                                                                                                                                 >  5:204637/1‑78 (MQ=255)                                                                                         tcAGGAATGCACCGGTTTGGCAAGTTCACGCAGAATTGTACGGAGGCATT‑CGCCGACGGA‑ATGCCTCCGTACAGACAt                                                                                                                                 <  6:204637/78‑1 (MQ=255)                                                                                           aGGAATGCACCGGTTTGGCAAGTTCACGCAGAATTGTACGGAGGCATT‑CGCCGACGGA‑ATGCCTCCGTACAGACATGACGTTGAATCAGCTGTTCTGCTCGAA‑‑CACCTCTTGGCCGGACAGCTCCTTGGTCTGATTGGCGAAGCAGTAc                                                      >  8:83475/1‑149 (MQ=255)                                                                                           aGGAATGCACCGGTTTGGCAACATCACGCAGAATTGTACGGAGGCATT‑CGCCGACGGA‑ATGCCTCCGTACAGACATGACGTTGAATCAGCTGTTCTGCTCGAA‑‑CACCTCTTGGCCGGACAGCTCCTTGGTCTGATTGGCGAAGCAGTAc                                                      >  8:332707/1‑149 (MQ=255)                                                                                              aaTGCACCGGTTTGGCAAGTTCACGCAGAATTGTACGGAGGCATT‑CGCCGACGGA‑ATGCCTCCGTACAGACATGACGTTGAATCAGCTGTTCTGCTCGAA‑‑CACCTCTTGGCCGGACAGCTCCTTGGTCTGATTGGCGAAGCAGTACCAc                                                   <  8:128656/149‑1 (MQ=255)                                                                                                  cACCGGTTTGGCAAGTTCACGCAGAATTGTACGGAGGCATT‑CGCCGACGGA‑ATGCCTCCGTACAGACATGACGTTGAATCAGCTGTTCTGCTCGAA‑‑CACCTCTTGGCCGGACAGCTCCTTGGTCTGATTGGCGAAGCAGTACCACGCgg                                               <  7:152327/149‑1 (MQ=255)                                                                                                   aCCGGTTTGGCAAGTTCACGCAGAATTGTACGGAGGCATT‑CGCCGACGGA‑ATGCCTCCGTACAGACATGACGTTGAATCAGCTGTTCTGCTCGAA‑‑CACCTCTTGGCCGGACAGCTCCTTGGTCTGATTGGCGAAGCAGTACCTCGCggg                                              <  2:226469/149‑1 (MQ=255)                                                                                                   aCCGGTTTGGCAAGTTCACGCAGAATTGTACGGAGGCATT‑CGCCGACGGA‑ATGCCTCCGTACAGACATGACGTTGAATCAGCTGTTCTGATCGAA‑‑CACCTCTTGGCCGGACAGATCCTTGGTCTCATTGGCGAAGCAGTACCACGCggg                                              >  3:156669/1‑149 (MQ=255)                                                                                                   aCCGGTTTGGCAAGTTCACGCAGAATTGTACGGAGGCATT‑CGCCGAAGGA‑ATTCCTCCGTACAGACATGACGTTGAATCAGCTGTTCTGATCGAA‑‑AACCTCTTGGCCGGACAGCTCCTTGGTCTGATTGGCGAAGCAGTACCACGCggg                                              >  2:190248/1‑149 (MQ=255)                                                                                                          tGGCAAGTTCACGCAGAATTGTACGGAGGCATT‑CGCCGACGGA‑ATGCCTCCGTACAGACATGACGTTGAATCAGCTGTTCTGCTCGAA‑‑CACCTCTTGGCCGGACAGCTCCTTGGTCTCATTGGCGAAGCAGTACCACGCGGGTTtctc                                        <  1:30399/148‑1 (MQ=255)                                                                                                            gCAAGTTCACGCAGAATTGTACGGAGGCATT‑CGCCGACGGA‑ATGCCTCCGTACAGACATGACGTTGAATCAGCTGTTCTGCTCGAA‑‑CACCTCTTGGCCGGACAGCTCCTTGGTCTGATTGGCGAAGCAGTACCACGCGGGTTTCTCTTc                                     <  6:98369/149‑1 (MQ=255)                                                                                                                    aCGCAGAATTGTACGGAGGCATT‑CGCCGACGGA‑ATGCCTCCGTACAGACATGACGTTGAATCAGCTGTTCTGCTCGAA‑‑CACCTCTTGGCCGGACAGCTCCTTGGTCTGATTgg                                                                 <  4:103361/113‑1 (MQ=255)                                                                                                                    aCGCAGAATTGTACGGAGGCATT‑CGCCGACGGA‑ATGCCTCCGTACAGACATGACGTTGAATCAGCTGTTCTGCTCGAA‑‑CACCTCTTGGCCGGACAGCTCCTTGGTCTGATTgg                                                                 >  3:103361/1‑113 (MQ=255)                                                                                                                      gCAGAATTGTACGGAGGCATT‑CGCCGACGGA‑ATGCCTCCGTACAGACATGACGTTGAATCAGCTGTTCTGCTCGAa‑‑c                                                                                                   <  4:115058/77‑1 (MQ=255)                                                                                                                      gCAGAATTGTACGGAGGCATT‑CGCCGACGGA‑ATGCCTCCGTACAGACATGACGTTGAATCAGCTGTTCTGCTCGAa‑‑c                                                                                                   >  3:115058/1‑77 (MQ=255)                                                                                                                       cAGAATTGTACGGAGGCATT‑CGCCGACGGA‑ATGCCTCCGTACAGACATGACGTTGAATCAGCTGTTCTGCTCGAA‑‑CACCTCTTGGCCGGACAGCTCCTTGGTCTGATTGGCGAAGCAGTACCACGCGGGTTTCTCTTCGACGAAGATCt                          <  3:105075/149‑1 (MQ=255)                                                                                                                            ttGTACGGAGGCATT‑CGCCGACGTA‑ATGCCTCCGTACAGACATGACGTTGAATCATCTGTTCTGCTCGAA‑‑CACCTCTTGGCCGGACAGCTCCTTGGTCTGATTGGCGAAGCAGTACCACGCGGGTTTCTCTTCGACGAAGATCTGCAgg                     <  7:332707/149‑1 (MQ=255)                                                                                                                                aCGGAGGCATT‑CGCCGACGGA‑ATGCCTCCGTACAGACATGACGTTGAATCAGCTGTTCTGCTCGAAGGAACCTCTTGGCCGGACAGCTCCTTGGTCTGATTGGCGAAGCAGTACCACGCGGGTTTCTCTTCGACGAAGATCTGCAGGTc                   >  1:110840/1‑149 (MQ=255)                                                                                                                                aCGGAGGCATT‑CGCCGACGGA‑ATGCCTCCGTACAGACATGACGTTGAATCAGCTGTTCTGCTCGAA‑‑CACCTCTTGGCCGGACAGCTCCTTGGTCTGATTGGCGAAGCAGTACCACGCGGGTTTCTCTTCGACGAAGATCTGCAGGTCg                  >  8:134339/1‑148 (MQ=255)                                                                                                                                 cGGAGGCATT‑CGCCGACGGA‑ATGCCTCCGTACAGACATGACGTTGAATCAGCTGTTCTGCTCGAA‑‑CACCTCTTGGCCGGACAGCTCCTTGGTCTGATTGGCGAAGCAGTACCACGCGGGTTTCTCTTCGACGAAGATCTGCAGGTCGa                 >  1:181783/1‑148 (MQ=255)                                                                                                                                  ggAGGCATTCCGTCGGC‑GA‑ATGCCTCCGTACAGACATGACGTTGAATCAGCTGTTCTGCTCGAA‑‑CACCTCTTgg                                                                                          >  3:246898/1‑74 (MQ=21)                                                                                                                                  ggAGGCATTCCGTCGGC‑GA‑ATGCCTCCGTACAGACATGACGTTGAATCAGCTGTTCTGCTCGAA‑‑CACCTCTTgg                                                                                          <  4:246898/74‑1 (MQ=21)                                                                                                                                  ggAGGCATTCCGTCGGC‑GA‑ATGCCTCCGTACAGACATGACGTTGAATCAGCTGTTCTGCTCGAA‑‑CACCTCTTGGCCGGACAGCTCCTTGGTCTGATTGGCGAAGCAGTACCACGCGGGTTTCTCTTc                                     <  1:293609/127‑1 (MQ=39)                                                                                                                                  ggAGGCATTCCGTCGGC‑GA‑ATGCCTCCGTACAGACATGACGTTGAATCAGCTGTTCTGCTCGAA‑‑CACCTCTTGGCCGGACAGCTCCTTGGTCTGATTGGCGAAGCAGTACCACGCGGGTTTCTCTTc                                     >  2:293609/1‑127 (MQ=39)                                                                                                                                    aGGCATT‑CGCCGACGGA‑ATGCCTCCGTACAGACATGACGTTGAATCAGCTGTTCTGCTCGAA‑‑CACCTCTTGGCCGGACAGCTCCTTGGTCTGATTGGCGAAGCAGTACCACGCGGGTTTCTCTTCGACGAAGATCTGCAGGTCGAAATc             >  4:6640/1‑149 (MQ=255)                                                                                                                                    aGGCATT‑CGCCGACGGA‑ATGCCTCCGTACAGACATGACGTTGAATCAGCTGTTCTGCTCGAA‑‑CACCTCTTGGCCGGACAGCTCCTTGGTCTGATTGGCGAAGCAGTACCACGCGGGTTTCTCTTCGACGAAGATCTGCAGGTCGAAATc             >  6:32168/1‑149 (MQ=255)                                                                                                                                       cATT‑CGCCGACGGA‑ATGCCTCCGTACAGACATGACGTTGAATCAGCTGTTCTGCTCGAA‑‑CACCTCTTGGCCGGACAGCTCCTTGGTCTGATTGGCGAAGCAGTACCACGCGGGTTTCTCTTCGACGAAGATCTGCAGGTCGAAATCCCa          <  3:6640/149‑1 (MQ=255)                                                                                                                                       cATT‑CGCCGACGGA‑ATGCCTCCGTACAGACATGACGTTGAATCAGCTGTTCTGCTCGAA‑‑CACCTCTTGGCCGGACAGCTCCTTGGTCTGATTGGCGAAGCAGTACCACGCGGGTTTCTCTTCGACGAAGATCTGCAGGTCGAAATCCCa          <  5:32168/149‑1 (MQ=255)                                                                                                                                        aTT‑CGCCGACGGA‑ATGCCTCCGTACAGACATGACGTTGAATCAGCTGTTCTGCTCGAA‑‑CACCTCTTGGCCGGACAGCTCCTTGGTCTGATTGGCGAAGCAGTACCACGCGGGTTTCTCTTCGACGAAGATCTg                         <  7:257158/133‑1 (MQ=255)                                                                                                                                        aTT‑CGCCGACGGA‑ATGCCTCCGTACAGACATGACGTTGAATCAGCTGTTCTGCTCGAA‑‑CACCTCTTGGCCGGACAGCTCCTTGGTCTGATTGGCGAAGCAGTACCACGCGGGTTTCTCTTCGACGAAGATCTg                         >  8:257158/1‑133 (MQ=255)                                                                                                                                         tt‑CGCCGACGGA‑ATGCCTCCGTACAGACATGACGTTGAATCAGCTGTTCTGCTCGAA‑‑CACCTCTTGGCCGGACAGCTCCTTGGTCTGATTGGCGAAGCAGTACCACGCGGGTTTCTCTTCGACGAAGATCTGCAGGTCGAAATCCCAgg        >  1:162632/1‑149 (MQ=255)                                                                                                                                             gCCGACGGA‑ATGCCTCCGTACAGACATGACGTTGAATCAGCTGTTCTGCTCGAA‑‑CACCTCTTGGCCGGACAGCTCCTTGGTCTGATTGGCGAAGCAGTACCACGCGGGTTTCTCTTCGACGAAGATCTGCAGGTCGAAATCCCAGGCtt     >  8:220193/1‑149 (MQ=255)                                                                                                                                             gCCGACGGA‑ATGCCTCCGTACAGACATGACGTTGAATCAGCTGTTCTGCTCGAA‑‑CACCTCTTGGCCGGACAGCTCCTTGGTCTGATTGGCGAAGCAGTACCACGCGGGTTTCTCTTCGACGAAGATCTGCAGGTCGAAATCCCAGGCtt     <  2:18158/149‑1 (MQ=255)                                                                                                                                               cGACGGA‑ATGCCTCCGTACAGACATGACGTTGAATCAGCTGTTCTGCTCGAA‑‑CACCTCTTGGCCGGACAGCTCCTTGGTCTGATAGGCGAAGCAGTACCACGCGGGTTTCTCTTCGACGAAGATCTGCAGGTCGAAATCCCAGGCTTgg   >  1:98917/1‑149 (MQ=255)                                                                                                                                                gACGGA‑ATGCCTCCGTACAGACATGACGTTGAATCAGCTGTTCTGCTCGAA‑‑CACCTCTTGGCCGGACAGCTCCTTGGTCTGATTGGCGAAGCAGTACCACGCGGGTTTCTCTTCGACGAAGATCTGCAGGTCGAAATCCCAGGCTTGGt  >  6:378558/1‑149 (MQ=255)                                                                                                                                                 |                                                                                                                                                       TTTGCCGTCTCCCTCGACCAGGTCGATGATCTGCAGTTCTTGGCTCACGGGTACACTCCAAACGCTGAAAAACAAAACCGCCCTTTTCTCAGGAATGCACCGGTTTGGCAAGTTCACGCAGAATTGTACGGAGGCATT‑CGCCGACGGA‑ATGCCTCCGTACAGACATGACGTTGAATCAGCTGTTCTGCTCGAA‑‑CACCTCTTGGCCGGACAGCTCCTTGGTCTGATTGGCGAAGCAGTACCACGCGGGTTTCTCTTCGACGAAGATCTGCAGGTCGAAATCCCAGGCTTGGT  >  NC_002947/4905359‑4905649

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑