| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 5,015,788 | C→G | 10.3% | intergenic (‑51/+183) | PP_5679 ← / ← PP_4421 | hypothetical protein/aminotransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 5,015,788 | 0 | C | G | 10.3% | 171.3 / 9.7 | 58 | intergenic (‑51/+183) | PP_5679/PP_4421 | hypothetical protein/aminotransferase |
| Reads supporting (aligned to +/- strand): ref base C (29/23); new base G (3/3); total (32/26) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.18e-01 | |||||||||||
CAGAGTGACTGCTGGGCACGAAGCTTCTCGGCAGCCCTGGACGTATAGGTAGCCACTGCTTCTTTGATCGGAGCCAGCTCGGTCATCCTAGCGCTCTCGTTTGAGTCCACGATGAGATGAATAAGCCCGCACCGGTCTCCGGCGCGGGCTTTTACTTTCCGGGCCTTGGACAGTATCTGACCCGAAAGTACGTCCTTGTGAACTGATGACAGCGTCTTGGCTTTGATTGTGATAGCACTACAAAAAAAAAAAGCCCGCCTAAAGGCGGGCTTAAAACGAAGAGG > NC_002947/5015652‑5015935 | cAGAGTGACTGCTGGGCACGAAGCTTCTCGGCAGCCCTGGACGTATAGGTAGCCACTGCTTCTTTGATCGGAGCCAGCTCGGTCATCCTAGCGCTCTCGTTTGAGTCCACGATGAGATGAATTAGCCCGCGCCGGAGACCGGTGCGGGc < 1:8194/149‑1 (MQ=255)cAGAGTGACTGCTGGGCACGAAGCTTCTCGGCAGCCCTGGACGTATAGGTAGCCACTGCTTCTTTGATCGGAGCCAGCTCGGTCATCCTAGCGCTCTCGTTTGAGTCCACGATGAGATGAATAAGCCCGCACCGGTCTCCGGCGCGGGc > 8:241945/1‑149 (MQ=255) agagTGACTGCTGGGCACGAAGCTTCTCGGCAGCCCTGGACGTATAGGTAGCCACTGCTTCTTTGATCGGAGCCAGCTCGGTCATCCTAGCGCTCTCGTTTGAGTCCACGATGAGATGAATAAGCCCGCACCGGTCTCCGGCGCCGGCt > 8:46168/1‑149 (MQ=255) gagTGACTGCTGGGCACGAAGCTTCTCGGCAGCCCTGGACGTATAGGTAGCCACTGCTTCTTTGATCGGAGCCAGCTCGGTCATCCTAGCGCTCTCGTTTGAGTCCACGATGAGATGAATAAGCCCGCGCCGGAGACCGGTGCgg < 1:358315/145‑1 (MQ=255) gagTGACTGCTGGGCACGAAGCTTCTCGGCAGCCCTGGACGTATAGGTAGCCACTGCTTCTTTGATCGGAGCCAGCTCGGTCATCCTAGCGCTCTCGTTTGAGTCCACGATGAGATGAATAAGCCCGCGCCGGAGACCGGTGCgg > 2:358315/1‑145 (MQ=255) agTGACTGCTGGGCACGAAGCTTCTCGGCAGCCCTGGACGTATAGGTAGCCACTGCTTCTTTGATCGGAGCCAGCTCGGTCATCCTAGCGCTCTCGTTTGAGTCCACGATGAGATGAATAAGCCCGCACCGGTCTCCGGCGCGGGCttt > 4:198618/1‑149 (MQ=255) aCTGCTGGGCACGAAGCTTCTCGGCAGCCCTGGACGTATAGGTAGCCACTGCTTCTTTGATCGGAGCCAGCTCGGTCATCCTAGCGCTCTCGTTTGAGTCCACGATGAGATGAATAAGCCCGCACCGGTCTCCGGCGCGGGCTTTTACt > 6:50093/1‑149 (MQ=255) ggCACGAAGCTTCTCGGCAGCCCTGGACGTATAGGTAGCCACTGCTTCTTTGATCGGAGCCAGCTCGGTCATCCTAGCGCTCTCGTTTGAGTCCACGATGAGATGAATAAGCCCGCACCGGTCTCCGGCGCGGGCTTTTACTTTCCgg < 5:50093/148‑1 (MQ=255) cGAAGCTTCTCGGCAGCCCTGGACGTATAGGTAGCCACTGCTTCTTTGATCGGAGCCAGCTCGGTCATCCTAGCGCTCTCGTTTGAGTCCACGATGAGATGAATAAGCCCGCACCGGTCTCCGGCGCGGGCTTTTACTTTCCGGGCCtt < 4:212602/149‑1 (MQ=255) gAAGCTTCTCGGCAGCCCTGGACGTATAGGTAGCCACTGCTTCTTTGATCGGAGCCAGCTCGGTCATCCTAGCGCTCTCGTTTGAGTCCACGATGAGATGAATAAGCCCCCACCGGTCTCCGGCGCGGGCTTTTACTTTCCGGGCCTTg > 4:11450/1‑149 (MQ=255) ttCTCGGCAGCCCTGGACGTATAGGTAGCCACTGCTTCTTTGATCGGAGCCAGCTCGGTCATCCTAGCGCTCTCGTTTGAGTCCACGATGAGATGAATAAGCCCGCACCGGTCTCCGGCGCGGGCTTTTACTTTCCGGGCCTTGGACAg > 4:272564/1‑149 (MQ=255) cTGGACGTATAGGTAGCCACTGCTTCTTTGATCGGAGCCAGCTCGGTCATCCTAGCGCTCTCGTTTGAGTCCACGATGAGATGAATAAGCCCGCACCGGTCTCCGGCGCGGGCTTTTACTTTCCGGGCCTTGGACAGTATCTGACCCg > 5:216551/1‑148 (MQ=255) gACGTATAGGTAGCCACTGCTTCTTTGATCGGAGCCAGCTCGGTCATCCTAGCGCTCTCGTTTGAGTCCACGATGAGATGAATAAGCCCGCACCGGTCTCCGGCGCGGGCtttt < 4:76676/114‑1 (MQ=255) gACGTATAGGTAGCCACTGCTTCTTTGATCGGAGCCAGCTCGGTCATCCTAGCGCTCTCGTTTGAGTCCACGATGAGATGAATAAGCCCGCACCGGTCTCCGGCGCGGGCTTTTACTTTCCGGGCCTTGGACAGTATCTGACCCGaaa < 6:101147/148‑1 (MQ=255) gACGAATAGGTAGCCACTGCTTCTTTGATCGGAGCCAGCTCGGTCATCCTAGCGCTCTCGTTTGAGTCCACGATGAGATGAATAAGCCCGCACCGGTCTCCGGCGCGGGCtttt > 3:76676/1‑114 (MQ=255) ataGGTAGCCACTGCTTCTTTGATCGGAGCCAGCTCGGTCATCCTAGCGCTCTCGTTTGAGTCCACGATGAGATGAATAAGCCCGCACCGGTCTCCGGCGCGGGCTTTTACTTTCCGGGCCTTGGACAGTATCTGACCCGAAAGTACGt > 4:69576/1‑149 (MQ=255) ggTAGCCACTGCTTCTTTGATCGGAGCCAGCTCGGTAATCCTAGCGCTCTCGTTTGAGTCCACTATGAGATGAATAAGCCCGCACCTGTCTCCGGCGCTGGCTTTTACTTTCCGGGCCTTGGACAGTATCTGACCCGAAAGTACGTcc < 2:194389/148‑1 (MQ=255) tAGCCACTGCTTATTTGATCGGAGCCAGCTCGGTCATCCTAGCGCTCTCGTTTGAGTCCACGATGAGATGAATAAGCCCGCACCGGTCTCCGGCGCGGGCTTTTACTTTCCGGGCCTTGGACAGTATCTGACCCGAAAGTACGTCCTtg < 6:72048/149‑1 (MQ=255) ccACTGCTTCTTTGATCGGAGCCAGCTCGGTCATCCTAGCGCTCTCGTTTGAGTCCACGATGAGATGAATAAGCCCGCACCGGTCTCCGGCGCGGGCTTTTACTTTCCGGGCCTTGGACAGTATCTGACCCGAAAGTACGTCCTTGTGa < 3:11450/149‑1 (MQ=255) cACTGCTTCTTTGATCGGAGCCAGCTCGGTCATCCTAGCGCTCTCGTTTGAGTCCACGATGAGATGAATAAGCCCGCACCGGTCTCCGGCGCGGGCTTTTACTTTCCGGGcc < 2:260454/112‑1 (MQ=255) cACTGCTTCTTTGATCGGAGCCAGCTCGGTCATCCTAGCGCTCTCGTTTGAGTCCACGATGAGATGAATAAGCCCGCACCGGTCTCCGGCGCGGGCTTTTACTTTCCGGGcc > 1:260454/1‑112 (MQ=255) aCTGCTTCTTTGATCGGAGCCAGCTCGGTCATCCTAGTGCTCTCGTTTGAGTCCACGATGAGATGAATAAGCCCGCACCGGTCTCCGGCGCGGGCTTTTACTTTCCGGGCCTTGGACAGTATCTGACCCGAAAGTACGTCCTTGTGAAc > 4:362637/1‑149 (MQ=255) aCTGCTTCTTTGATCGGAGCCAGCTCGGTCATCCTAGCGCTCTCGTTTGAGTCCACGATGAGATGAATAAGCCCGCACCGGTCTCCGGCGCGGGCTTTTACTTTCCGGGCCTTGGACAGTATCTGACCCGAAAGTACGTCCTTGTGAAc > 6:142378/1‑149 (MQ=255) aCTGCTTCTTTGATCGGAGCCAGCTCGGTCATCCTAGCGCTCTCGTTTGAGTCCACGATGAGATGAATAAGCCCGCACCGGTCTCCGGCGCGGGCTTTTACTTTCCGGGCCTTGGACAGTATCTGACCCGAAAGTACGTCCTTGTGAAc > 6:197041/1‑149 (MQ=255) cttcttTGATCGGAGCCAGCTCGGTCATCCTAGCGCTCTCGTTTGAGTCCACGATGAGATGAATAAGCCCGCACCGGTCTCCGGCGCGGGCTTTTACTTTCCGGGCCTTGGACAGTATCTGAc > 6:13867/1‑123 (MQ=255) cttcttTGATCGGAGCCAGCTCGGTCATCCTAGCGCTCTCGTTTGAGTCCACGATGAGATGAATAAGCCCGCACCGGTCTCCGGCGCGGGCTTTTACTTTCCGGGCCTTGGACAGTATCTGAc < 5:13867/123‑1 (MQ=255) tcttTGATCGGAGCCAGCTCGGTCATCCTAGCGCTCTCGTTTGAGTCCACGATGAGATGAATAAGCCCGCACCGGTCTCCGGCGCGGGCTTTTACTTTCCGGGCCTTGGACAGTATCTGACCCGAAAGTACGTCCTTGTGAACtgatga > 5:123014/1‑149 (MQ=255) gATCGGAGCCAGCTCGGTCATCCTAGCGCTCTCGTTTGAGTCCACGATGAGATGAATAAGCCCGCACCGGTCTCCGGCGCGGGCTTTTACTTTCCGGGCCTTGGACAGTATCTGACCCGAAAGTACGTCCTTGTGAACTGATGACAGc < 3:198618/148‑1 (MQ=255) gATCGGAGCCAGCTCGGTCATCCTAGCGCTCTCGTTTGAGTCCACGATGAGATGAATAAGCCCGCACCGGTCTCCGGCGCGGGCTTTTACTTTCCGGGCCTTGGACAGTATCTGACCCGAAAGTACGTCCTTGTGAACTGATGACAGCg > 3:352334/1‑149 (MQ=255) gATCGGAGCCAGCTCGGTCATCCTAGCGCTCTCGTTTGAGTCCACGATGAGATGAATAAGCCCGCACCGGTCTCCGGCGCGGGCTTTTACTTTCCGGGCCTTGGACAGTATCTGACCCGAAAGTACGTCCTTGTGAACTGATGACAGCg > 3:99693/1‑149 (MQ=255) aTCGGAGCCAGCTCGGTCATCCTAGCGCTCTCGTTTGAGTCCACGATGAGATGAATAAGCCCGCACCGGTCTCCGGCGCGGGCTTTTACTTTCCGGGCCTTGGACAGTATCTGACCCGAAAGTACGTCCTTGTGAACTGATGACAGCGt > 5:186572/1‑149 (MQ=255) tCGGAGCCAGCTCGGTCATCCTAGCGCTCTCGTTTGAGTCCACGATGAGATGAATAAGCCCGCGCCGGAGACCGGTGCGGGCt > 1:245461/1‑83 (MQ=37) tCGGAGCCAGCTCGGTCATCCTAGCGCTCTCGTTTGAGTCCACGATGAGATGAATAAGCCCGCGCCGGAGACCGGTGCGGGCt < 2:245461/83‑1 (MQ=37) ggAGCCAGCTCGGTCATCCTAGCGCTCTCGTTTGAGTCCACGATGAGATGAATAAGCCCGCACCGGTCTCCGGCGCGGGCTTTTACTTTCCGGGCCTTGGACAGTATCTGACCCGAAAGTACGTCCTTGTGAACTGATGACAGCGTCtt > 8:144857/1‑149 (MQ=255) cTCGGTCATCCTAGCGCTCTCGTTTGAGTCCACGATGAGATGAATAAGCCCGCACCGGTCTCCGGCGCGGGCTTTTACTTTCCGGGCCTTGGACAGTATCTGACCCGAAAGTACGTCCTTGTGAACTGATGACAGCGTCTTGGCTTTGa < 6:186572/149‑1 (MQ=255) ggTCATCCTAGCGCTCTCGTTTGAGTCCACGATGAGATGAATAAGCCCGCACCGGTCTCCGGCGCGGGCTTTTACTTTCCGGGCCTTGGACAGTATCTGACCCGAAAGTACGTCCTTGTGAACTGATGACAGCGTCTTGGCTTTGAtt < 6:44167/148‑1 (MQ=255) ggTCATCCTAGCGCTCTCGTTTGAGTCCACGATGAGATGAATAAGCCCGCACCGGTCTCCGGCGCGGGCTTTTACTTTCCGGGCCTTGGACAGTATCTGACCCGAAAGTACGTCCTTGTGAACTGATGACAGCGTCTTGGCTTTGATtg < 6:123014/149‑1 (MQ=255) gTCATCCTAGCGCTCTCGTTTGAGTACACGATGAGATGAATAATCCCGCTCCGGTCTCCGGCGCGGGCTTTTACTTTCAGGGCCTTGGAGAGTATCTGACCCGAAAGTACGTCCTTGTGAACTGATGACAGCGTCTTGTCTTTGATtgt < 4:99693/149‑1 (MQ=255) tCATCCTAGCGCTCTCGTTTGAGTCCACGATGAGATGAATAAGCCCGCACCGGTCTCCGGCGCGGGCTTTTACTTTCCGGGCCTTGGACAGTATCTGACCCGAAAGTACGTCCTTGTGAACtgatga < 3:145647/127‑1 (MQ=255) tCATCCTAGCGCTCTCGTTTGAGTCCACGATGAGATGAATAAGCCCGCACCGGTCTCCGGCGCGGGCTTTTACTTTCCGGGCCTTGGACAGTATCTGACCCGAAAGTACGTCCTTGTGAACtgatga > 4:145647/1‑127 (MQ=255) tCATCCTAGCGCTCTCGTTTGAGTCCACGATGAGATGAATAAGCCCGCACCGGTCTCCGGCGCGGGCTTTTACTTTCCGGGCCTTGGACAGTATCTGACCCGAAAGTACGTCCTTGTGAACTGATGACAGCATCTTGGCTTTGATtgt > 6:180107/1‑148 (MQ=255) tCCTAGTGCTCTCGTTTGAGTCCACGATGAGATGAATAAGCCCGCACCGGTCTCCGGCGCGGGCTTTTACTTTCCGGGCCTTGGACAGTATCTGACCCGAAAGTACGTCCTTGTGAACTGATGACAGCGTCTTGGCTTTGATTGTGATa < 3:362637/149‑1 (MQ=255) tCCTAGCGCTCTCGTTTGAGTCCACGATGAGATGAATAAGCCCGCACCGGTCTCCGGCGCGGGCTTTTACTTTCCGGGCCTTGGACAGTATCTGACCCGAAAGTACGTCCTTGTGAACTGATGACAGCGTCTTGGCTTTGATTGTGATa < 5:142378/149‑1 (MQ=255) cTAGCGCTCTCGTTTGAGTCCACGATGAGATGAATCAGCCCGCACCGGTCTCCGGCGCGGGCTTTTACTTTCCGGGCCTTGGACAGTATCTGACCCGAAAGTACGTCCTTGTGAACTGATGACAGCGTCTTGGCTTTGATTGTGATAGc < 7:241945/149‑1 (MQ=255) cTAGCGCTCTCGTTTGAGTCCACGATGAGATGAATAAGCCCGCACCGGTCTCCGGCGCGGGCTTTTACTTTCCGGGCCTTGGACAGTATCTGACCCGAAAGTACGTCCTTGTGAACTGATGACAGCGTCTTGGCTTTGATTGTGATAGc > 2:288308/1‑149 (MQ=255) tAGCGCTCTCGTTTGAGTCCACGATGAGATGAATAAGCCCGCACCGGTCTCCGGCGCGGGCTTTTACTTTCCGGGCCTTGGACAGTATCTGACCCGAAAGTACGTCCTTGTGAACTGATGACAGCGTCTTGGCTTTGATTGTGATAGc > 7:97424/1‑148 (MQ=255) ctctCGTTTGAGTCCACGATGAGATGAATAAGCCCGCACCGGTCTCCGGCGCGGGCTTTTACTTTCCGGGCCTTGGACAGTATCTGACCCGAAAGTACGTCCTTGTGAACTGATGACAGCGTCTTGGCTTTGATTGTGATAGCACTAc > 3:260392/1‑148 (MQ=255) tCCACGATGAGATGAATAAGCCCGCACCGGTCTCCGGCGCGGGCTTTTACTTTCCGGGCCTTGGACAGTATCTGACCCGaaa > 3:67720/1‑82 (MQ=255) tCCACGATGAGATGAATAAGCCCGCACCGGTCTCCGGCGCGGGCTTTTACTTTCCGGGCCTTGGACAGTATCTGACCCGaaa < 4:67720/82‑1 (MQ=255) aCGATGAGATGAATAAGCCCGCACCGGTCTCCGGCGCGGGCTTTTACTTTCCGGGCCTTGGACAGTATc > 3:277145/1‑69 (MQ=255) aCGATGAGATGAATAAGCCCGCACCGGTCTCCGGCGCGGGCTTTTACTTTCCGGGCCTTGGACAGTATc < 4:277145/69‑1 (MQ=255) gtaaaaGCCCGCGCCGGAGACCGGTGCGGGCTTTTACTTTCCGGGCCTTGGATAGTATCTGACCCGAAAGTACGTCCTTGTGAACTGATGACAGCGTCTTGGCTTTGATTGTGATAGCACTACAAAAAAAAAAAGCCCGCCAAAAGGCg > 2:106936/5‑149 (MQ=255) aaTAAGCCCGCACCGGTCTCCGGCGCGGGCTTTTACTTTCCGGGCCTTGGACAGTATCTGACCCGAAAGTACGTCCTTGTGAACTGATGACAGCGTCTTGGCTTTGATTGTGATAGCACTAC‑AAAAAAAAAAGCCCGCCTAAAGGCgg < 3:69576/148‑1 (MQ=255) aaTAACCCCGCACCGGGCTCCGGCGCGGGGTTTTAATTTCCGGGGCCTGGACATTATCTTACCCGAAAGTACGTCCTTGTGAACTGATTACATCGTCTTGGCTTTTTTTTTGATACCACTAAAAAAAAAAAAAGCCCGCCTAAAGGCg < 6:216551/148‑1 (MQ=255) aTAAGCCCGCACCGGTCTCCGGCGCGGGCTTTTACTTTCCGGGCCTTGGACAGTATCTGACCCGAAAGTACGTCCTTGTGAACTGATGACAGCGTCTTGGCTTTGATTGTGATAGCACTAC‑AAAAAAAAAAGCCCGCCTAAAGGCggg < 4:260392/148‑1 (MQ=255) cGCACCGGTCTCCGGCGCGGGCTTTTACTTTCCGGGCCTTTGACAGTATCTGACCCGAAAGTACGTCCTTGTGAACTGATGACAGCGTCTTGGCTTTGATTGTGATAGCACTACAAAAAAAAAAA‑CCCGCCTAAAGGCGGCCTTAAAAc > 6:349164/1‑149 (MQ=255) gCACCGGTCCCCGGCGCGGGCTTTTACTTTCCGGGCCTTGGACAGTATCTGACACGAAAGTACGTCCTTGTGAACTGATGACAGCGTCTTGGCTTTGATTGAGATAGCACTACAAAAAAAAAAACCCACCCTAAATGCGGGCTTAAAAc > 4:125224/1‑149 (MQ=255) cGGTCTCCGGCGCGGGCTTTTACTTTCAGGGCCTTGGACAGTATCTGACCAGAAAGTAAGACCTAGTGAACTGATGACAGCGTCTTGGCTTTGATTGTGATAGCACTACAAAAAAAAAAAGCACGCCGAAAGGCCGGAGTAAAACg > 4:10907/1‑146 (MQ=255) tctcCGGCGCGGGCTTTTACTTTCCGGGCCTTGGACAGTATCTGACCCGAAATTACGTCCTTGTGAACTGATGACAGCGTCTTGGCTTTGATTGTGATAGCACTACAAAAAAAAAAAGCCCGCCTAAAGGCGGGCTTAAAACGAAGAgg < 3:272564/149‑1 (MQ=255) | CAGAGTGACTGCTGGGCACGAAGCTTCTCGGCAGCCCTGGACGTATAGGTAGCCACTGCTTCTTTGATCGGAGCCAGCTCGGTCATCCTAGCGCTCTCGTTTGAGTCCACGATGAGATGAATAAGCCCGCACCGGTCTCCGGCGCGGGCTTTTACTTTCCGGGCCTTGGACAGTATCTGACCCGAAAGTACGTCCTTGTGAACTGATGACAGCGTCTTGGCTTTGATTGTGATAGCACTACAAAAAAAAAAAGCCCGCCTAAAGGCGGGCTTAAAACGAAGAGG > NC_002947/5015652‑5015935 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |