Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,041,195 C→G 7.5% intergenic (+173/‑489) PP_5680 → / → PP_4447 Cro/CI family transcriptional regulator/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,041,1950CG 7.5% 290.2 / 8.5 90intergenic (+173/‑489)PP_5680/PP_4447Cro/CI family transcriptional regulator/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (43/40);  new base G (2/5);  total (45/45)
Fisher's exact test for biased strand distribution p-value = 4.34e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ACACTTGTACTCCACACCCAGTCTCACATCTGACCCTGTCAGCGACGCTAATGACGGTGTTGATCCACAGAGGGTTAGGGATAGCCTTAAGGCGTAATTGTCTGATACCAACACCTCTGCGATGAGGACATGACCTCTTCATCGG‑CGCGCCTTAAGCCCGCCGA‑GAAGAGCCAGAGACCCCGAGCAAGACCCCTAACCGCCTTATGGCGGTTTCTCTGCCTCCCAGTCTGCTGCGAAGGCCGATAGCCGAGTCCAACCAATTCTATTTTTCGACCTGGCCTGACCGCAGTAGAGC  >  NC_002947/5041048‑5041342
                                                                                                                                                    |                                                                                                                                                    
acacTTGTACTCCACACCCAGTCTCACATCTGACCCTGTCAGCGACGCTAATGACGGTGTTGATCCACAGAGGGTTAGGGATAGCCTTAAGGCGTAATTGTCTGATACCAACACCTCTGCGATGAGGACATGACCTCTTCATCGg‑cgcg                                                                                                                                                     >  2:256903/1‑149 (MQ=255)
  acTTGTACTCCACACCCAGTCTCACATCTGACCCTGTCAGCGACGCTAATGACGGTGTTGATCCACAGAGGGTTAGGGATAGCCTTAAGGCGTAATTGTCTGATACCAACACCTCTGCGATGAGGACATGACCTCTTCATCGG‑CGCGcc                                                                                                                                                   >  6:8284/1‑149 (MQ=255)
    ttGTACTCCACACCCAGTCTCACATCTGACCCTGTCAGCGACGCTAATGACGGTGTTGATCCACAGAGGGTTAGGGATAGCCTTAAGGCGTAATTGTCTGATACCAACACCTCTGCGATGAGGACATGACCTCTTCATCGG‑CGCGCCt                                                                                                                                                  >  2:205182/1‑148 (MQ=255)
      gTACTCCACACCCAGTCTCACATCTGACCCTGTCAGCGACGCTAATGACGGTGTTGATCCACAGAGGGTTAGGGATAGCCTTAAGGCGTAATTGTCTGATACCAACACCTCTGCGATGAGGACATGACCTCTTCATCGG‑CGCGCCtt                                                                                                                                                 >  1:276225/1‑147 (MQ=255)
            cacaCCCAGTCTCACATCTGACCCTGTCAGCGACGCTAATGACGGTGTTGATCCACAGAGGGTTAGGGATAGCCTTAAGGCGTAATTGTCTGATACCAACACCTCTGCGATGAGGACATGACCTCTTCATCGG‑CGCGCCTTAAGCccgc                                                                                                                                         >  6:89605/1‑149 (MQ=255)
                cccAGTCTCACATCTGACCCTGTCAGCGACGCTAATGACGGTGTTGATCCACAGAGGGTTAGGGATAGCCTTAAGGCGTAATTGTCTGATACCAACACCTCTGCGATGAGGACATGACCTCTTCATCGG‑CGCGCCTTAAGCccgcc                                                                                                                                        <  6:70333/146‑1 (MQ=255)
                cccAGTCTCACATCTGACCCTGTCAGCGACGCTAATGACGGTGTTGATCCACAGAGGGTTAGGGATAGCCTTAAGGCGTAATTGTCTGATACCAACACCTCTGCGATGAGGACATGACCTCTTCATCGG‑CGCGCCTTAAGCccgcc                                                                                                                                        >  5:70333/1‑146 (MQ=255)
                   aGTCTCACATCTGACCCTGTCAGCGACGCTAATGACGGTGTTGATCCACAGAGGGTTAGGGATAGCCTTAAGGCGTAATTGTCTGATACCAACACCTCTGCGATGAGGACATGACCTCTTCATCGG‑CGGGCTTAAGGCGCGCCgatgaa                                                                                                                                  <  8:183197/149‑5 (MQ=255)
                    gTCTCACATCTGACCCTGTCAGCGACGCTAATGACGGTGTTGATCCACAGAGGGTTAGGGATAGCCTTAAGGCGTAATTGTCTGATACCAACACCTCTGCGATGAGGACATGACCTCTTCATCGG‑CGCGCCTTAAGCCCGCCGa‑gaaga                                                                                                                                <  2:276225/149‑1 (MQ=255)
                    gTCTCACATCTGACCCTGTCAGCGACGCTAATGACGGTGTTGATCCACAGAGGGTTAGGGATAGCCTTAAGGCGTAATTGTCTGATACCAACACCTCTGCGATGAGGACATGACCTCTTCATCGG‑CGCGCCTTAAGCCCGCCGa‑gaaga                                                                                                                                <  8:143623/149‑1 (MQ=255)
                    gTCTCACATCTGACCCTGTCAGCGACGCTAATGACGGTGTTGATCCACAGAGGGTTAGGGATAGCCTTAAGGCGTAATTGTCTGATACCAACACCTCTGCGATGAGGACATGACCTCTTC‑TCGG‑CGGGCTTAAGGCGCGCCGATgaaga                                                                                                                                <  3:149848/149‑1 (MQ=38)
                     tctcACATCTGACCCTGTCAGCGACGCTAATGACGGTGTTGATCCACAGAGGGTTAGGGATAGCCTTAAGGCGTAATTGTCTGATACCAACACCTCTGCGATGAGGACATGACCTCTTCATCGG‑CGCGCCTTAAGCCCGCCGA‑GAagag                                                                                                                               >  8:197380/1‑149 (MQ=255)
                       tcACATCTGACCCTGTCAGCGACGGTAATGACGGTGTTGATCCACAGAGGGTTAGGGATAGCCTTAAGGCGTAATTGTCTGATACCAACACCTCTGCGATGAGGACATGACCTCTTCATCGG‑CGCGCCTTAAGCCCGCCGA‑GAAGAGc                                                                                                                              >  7:52526/1‑148 (MQ=255)
                       tcACATCTGACCCTGTCAGCGACGCTAATGACGGTGTTGATCCACAGAGGGTTAGGGATAGCCTTAAGGCGTAATTGTCTGATACCAACACCTCTGCGATGAGGACATGACCTCTTCATCGG‑CGCGCCTTAAGCCCGCCGA‑GAataga                                                                                                                              >  8:146935/1‑147 (MQ=255)
                        cacaTCTGACCCTGTCAGCGACGCTAATGACGGTGTTGATCCACAGAGGGTTAGGGATAGCCTTAAGGCGTAATTGTCTGATACCAACACCTCTGCGATGAGGACATGACCTCTTCATCGG‑CGCGCCTTAAGCCCGCCGA‑GAAGAGc                                                                                                                              >  5:241560/1‑147 (MQ=255)
                         acaTCTGACCCTGTCAGCGACGCTAATGACGGTGTTGATCCACAGAGGGTTAGGGATAGCCTTAAGGCGTAATTGTCTGATACCAACACCTCTGCGATGAGGACATGACCTCTTCATCGGCCGCGCCTTAAGCCCGCCGA‑GAAGAGCCa                                                                                                                            <  1:205182/149‑1 (MQ=255)
                         acaTCTGACCCTGTCAGCGACGCTAATGACGGTGTTGATCCACAGAGGGTTAGGGATAGCCTTAAGGCGTAATTGTCTGATACCAACACCTCTGCGATGAGGACATGACCTCTTCATCGG‑CGCGCCTTAAGCCCGCCGA‑GAAGAGc                                                                                                                              >  6:139066/1‑146 (MQ=255)
                         acaTCTGACCCTGTCAGCGACGCTAATGACGGTGTTGATCCACAGAGGGTTAGGGATAGCCTTAAGGCGTAATTGTCTGATACCAACACCTCTGCGATGAGGACATGACCTCTTCATCGG‑CGCGCCTTAAGCCCGCCGA‑GAAGAGCCa                                                                                                                            <  8:52526/148‑1 (MQ=255)
                         aaaTCTGACCCTGTCAGCGACCCTAATGACGGTGTTGATCCAGAGAGGGTTAGGGAAATCCTTAAGGCTTAATTGTCTGATACCAACTCCTCTGCGATGATTACATGACATCTTCATCGT‑CGGGCCTTAAGCCCTCAGA‑GAATAGCCag                                                                                                                           <  8:341501/147‑1 (MQ=255)
                          caTCTGACCCTGTCAGCGACGCTAATGACGGTGTTGATCCACAGAGGGTTAGGGATAGCCTTAAGGCGTAATTGTCTGATACCAACACCTCTGCGATGAGGACATGACCTCTTC‑TCGG‑CGGGCTTAAGGCGCGCCgatgaa                                                                                                                                  <  1:334048/141‑5 (MQ=255)
                          caTCTGACCCTGTCAGCGACGCTAATGACGGTGTTGATCCACAGAGGGTTAGGGATAGCCTTAAGGCGTAATTGTCTGATACCAACACCTCTGCGATGAGGACATGACCTCTTC‑TCGG‑CGGGCTTAAGGCGCGCCgatgaa                                                                                                                                  >  2:334048/1‑137 (MQ=255)
                           aTCTGACCCTGTCAGCGACGCTAATGACGGTGTTGATCCACAGAGGGTTAGGGATAGCCTTAAGGCGTAATTGTCTGATACCAACACCTCTGCGATGAGGACATGACCTCTTCATCGG‑CGCGCCTTAAGCCCGCCGA‑GAAGAGCCagag                                                                                                                         >  8:276134/1‑149 (MQ=255)
                                aCCCTGTCAGCGACGCTAATGACGGTGTTGATCCACAGAGGGTTAGGGATAGCCTTAAGGCGTAATTGTCTGATACCAACACCTCTGCGATGAGGACATGACCTCTTCATCGG‑CGCGCCTTAAGCCCGCCGA‑GAAGAGCCAGAGAcccc                                                                                                                    >  1:305695/1‑149 (MQ=255)
                                  ccTGTCAGCGACGCTAATGACGGTGTTGATCCACAGAGGGTTAGGGATAGCCTTAAGGCGTAATTGTCTGATACCAACACCTCTGCGATGAGGACATGACCTCTTCTTCGG‑CGCGCCTTAAGCCCGCCGA‑GAAGAGCCAGAGACCCCGa                                                                                                                  <  1:256903/149‑1 (MQ=255)
                                  ccTGTCAGCGACGCTAATGACGGTGTTGATCCACAGAGGGTTAGGGATAGCCTTAAGGCGTAATTGTCTGATACCAACACCTCTGCGATGAGGACATGACCTCTTCATCGG‑CGCGCCTTaa                                                                                                                                               >  2:294694/1‑121 (MQ=255)
                                  ccTGTCAGCGACGCTAATGACGGTGTTGATCCACAGAGGGTTAGGGATAGCCTTAAGGCGTAATTGTCTGATACCAACACCTCTGCGATGAGGACATGACCTCTTCATCGG‑CGCGCCTTaa                                                                                                                                               <  1:294694/121‑1 (MQ=255)
                                  ccTGTCAGCGACGCTAATGACGGTGTTGATCCACAGAGGGTTAGGGATAGCCTTAAGGCGTAATTGTCTGATACCAACACCTCTGCGATGAGGACATGACCTCTTCATCGG‑CGCGCCTTAAGCCCGCCGA‑GAAGAGCCAGAGACCCCGa                                                                                                                  >  2:116590/1‑149 (MQ=255)
                                  ccTGTCAGCGACGCTAATGACGGTATTGATCCACAGAGGGTTTGGGAAAGCCTTAAGGCGTAATTTTCTGATACCAACACCTCTGGGATGAGGACATGACCTCTTCAACCG‑CGCGCCTTAAGCCCGCCGA‑GAAAAGCCAGAGCcccccc                                                                                                                  >  7:52639/1‑147 (MQ=255)
                                   cTGTCAGCGACGCTAATGACGGTGTTGATCCACAGAGGGTTAGGGATAGCCTTAAGGCGTAATTGTCTGATACCAACACCTCTGCGATGAGGACATGACCTCTTCATCGG‑CGCGCCTTAAGCCCGCCGA‑GAAGAGCCAGAGACCCCGAg                                                                                                                 <  1:33976/149‑1 (MQ=255)
                                       cAGCGACGCTAATGACGGTGTTGATCCACAGAGGGTTAGGGATAGCCTTAAGGCGTAATTGTCTGATACCAACACCTCTGCGATGAGGACATGACCTCTTCATCGG‑CGCGCCTTAAGACAGCCGA‑GAAGAGCCAGAGACCCCGAGc                                                                                                                >  8:307353/1‑146 (MQ=255)
                                        aGCGACGCTAATGACGGTGTTGATCCACAGAGGGTTAGGGATAGCCTTAAGGCGTAATTGTCTGATACCAACACCTCTGCGATGAGGACATGACCTCTTCATCGG‑CGCGCCTTAAGCCCGCCGA‑GAAGAGCCAGAGACCCCGAGCAAGa                                                                                                            <  2:305695/149‑1 (MQ=255)
                                         gCGACGCTAATGACGGTGTTGATCCACAGAGGGTTAGGGATAGCCTTAAGGCGTAATTGTCTGATACCAACACCTCTGCGATGAGGACATGACCTCTTCATCGG‑CGCGCCTTAAGCCCGCCGA‑GAAGAGCCAGAGACCCCGAGCAAGAc                                                                                                           >  1:135736/1‑149 (MQ=255)
                                         gCGACGCTAATGACGGTGTTGATCCACAGAGGGTTAGGGATAGCCTTAAGGCGTAATTGTCTGATACCAACACCTCTGCGATGAGGACATGACCTCTTCATCGG‑CGCGCCTTAAGCCCGCCGA‑GAAGAGCCAGAGACCCCGAGCAAGAc                                                                                                           <  5:89605/149‑1 (MQ=255)
                                           gACGCTAATGACGGTGTTGATCCACAGAGGGTTAGGGATAGCCTTAAGGCGTAATTGTCTGATACCAACACCTCTGCGATGAGGACATGACCTCTTCATCGG‑CGCGCCTTAAGCCCGCCGA‑GAAGAGCCAGAGACCCCGAGCAAGAccc                                                                                                         <  6:155051/149‑1 (MQ=255)
                                            aCGCTAATGACGGTGTTGATCCACAGAGGGTTAGGGATAGCCTTAAGGCGTAATTGTCTGATACCAACACCTCTGCGATGAGGACATGACCTCTTCATCGG‑CGCGCCTTAAGCCCGCCGA‑GAAGAGCCAGAGACCCCGAGCAAGAcccc                                                                                                        <  3:170863/149‑1 (MQ=255)
                                                tAATGACGGTGTTGATCCACAGAGGGTTAGGGATAGCCTTAAGGCGTAATTGTCTGATACCAACACCTCTGCGATGAGGACATGACCTCTTCATCGG‑CGCGCCTTAAGCCCGCCGA‑GAAGAGCCAGAGACCCCGAGCAAGACCCCTAAc                                                                                                    >  3:362680/1‑149 (MQ=255)
                                                tAATGACGGTGTTGATCCACAGAGGGTTAGGGATAGCCTTAAGGCGTAATTGTCTGATACCAACACCTCTGCGATGAGGACATGACCTCTTCATCGG‑CGCGCCTTAAGCCCGCCGA‑GAAGAGCCAGAGACCCCGAGCAAGACCCCTAAc                                                                                                    >  2:307907/1‑149 (MQ=255)
                                                tAATGACGGTGTTGATCCACAGAGGGTTAGGGATAGCCTTAAGGCGTAATTGTCTGATACCAACACCTCTGCGATGAGGACATGACCTCTTAATCGG‑CGCGCCTTAAGCCCGCCGA‑GAAGAGCCAGAGACCCCGAGCAACACACCTAAc                                                                                                    >  1:184448/1‑149 (MQ=255)
                                                    gACGGTGTTGATCCACAGAGGGTTAGGGATAGCCTTAAGGCGTAATTGTCTGATACCAACACCTCTGCGATGAGGACATGACCTCTTCATCGG‑CGCGCCTTAAGCCCGCCGA‑GAAGAGCCAGAGACCCCGAGCAAGACCCCTAACCGcc                                                                                                >  6:274469/1‑149 (MQ=255)
                                                     aCGGTGTTGATCCACAGAGGGTTAGGGATAGCCTTAAGGCGTAATTGTCTGATACCAACACCTCTGCGATGAGGACATGACCTCTTCATCGG‑CGCGCCTTAAGCCCGCCGA‑GAAGAGCCAGAGACCCCGAGCAAGACCCCTAACCGCCt                                                                                               >  6:273753/1‑149 (MQ=255)
                                                     aCGGTGTTGATCCACAGAGGGTTAGGGATAGCCTTAAGGCGTAATTGTCTGATACCAACACCTCTGCGATGAGGACATGACCTCTTCATCGG‑CGCGCCTTAAGCCCGCCGA‑GAAGAGCCAGAGACCCCGAGCAAGACCCCTAACCGCCt                                                                                               >  4:198455/1‑149 (MQ=255)
                                                       ggTGTTGATCCACAGAGGGTTAGGGATAGCCTTAAGGCGTAATTGTCTGATACCCACACCTCTGCGATGAGGACATGACCTCTTCATCGG‑CGCGCCTTAAGCCCGCCGA‑GAAGAGCCAGAGACCCCGAGCAAGACCCCTAACCGCCTTa                                                                                             >  8:281611/1‑149 (MQ=255)
                                                               tCCACAGAGGGTTAGGGATAGCCTTAAGGCGTAATTGTCTGATACCAACACCTCTGCGATGAGGACATGACCTCTTCATCGG‑CGCGCCTTAAGCCCGCCGA‑GAAGAGCCAGAGACCCCGAGCAAGACCCCTAACCGCCATAAGGCGGt                                                                                      <  3:66048/148‑1 (MQ=255)
                                                                 cacaGAGGGTTAGGGATAGCCTTAAGGCGTAATTGTCTGATACCAACACCTCTGCGATGAGGACATGACCTCTTCATCGG‑CGCGCCTTAAGCCCGCCGA‑GAAGAGCCAGAGACCCCGAGCAAGACCCCTAACCGCCTTATGGCGGTTtc                                                                                   >  7:6954/1‑149 (MQ=255)
                                                                  acaGAGGGTTAGGGATAGCCTTAAGGCGTAATTGTCTGATACCAACACCTCTGCGATGAGGACATGACCTCTTCATCGG‑CGCGCCTTAAGCCCGCCGA‑GAAGAGCCAGAGACCCCGAGCAAGACCCCTAACCGCCTTATGGCGGTTtct                                                                                  <  5:8284/149‑1 (MQ=255)
                                                                   caGAGGGTTAGGGATAGCCTTAAGGCGTAATTGTCTGATACCAACACCTCTGCGATGAGGACATGACCTCTTCATCGG‑CGCGCCTTAAGCCCGCCGA‑GAAGAGCCAGAGACCCCGAGCAAGACCCCTAACCGCCTTATGGCGGTTtctc                                                                                 <  3:125378/149‑1 (MQ=255)
                                                                    agagGGTTAGGGATAGCCTTAAGGCGTAATTGTCTGATACCAACACCTCTGCGATGAGGACATGACCTCTTCATCGG‑CGCGCCTTAAGCCCGCCGA‑GAAGAGCCAGAGACCCCGAGCAAGACCCCTAACCGCCTTATGGCGGTTTctct                                                                                <  7:276134/149‑1 (MQ=255)
                                                                        ggTTAGGGATAGCCTTAAGGCGTAATTGTCTGATACCAACACCTCTGCGATGAGGACATGACCTCTTCATCGG‑CGCGCCTTAAGCCCGCCGA‑GAAGAGCCAGAGACCCCGAGCAAGACCCCTAACCGCCTTATGGCGGTTTCTCTGCCt                                                                            <  7:146935/149‑1 (MQ=255)
                                                                          ttAGGGATAGCCTTAAGGCGTAATTGTCTGATACCAACACCTCTGCGATGAGGACATGACCTCTTCATCGG‑CGCGCCTTAAGCCCGCCGA‑GAAGAGCCAGAGACCCCGAGCAAGACCCCTAACCGCCTTATGGCGGTTTCTCTGCCTcc                                                                          <  1:116590/149‑1 (MQ=255)
                                                                             gggATAGCCTTAAGGCGTAATTGTCTGATACCAACACCTCTGCGATGAGGACATGACCTCTTCATCGG‑CGCGCCTTAAGCCCGCCGA‑GAAGAGCCAGAGACCCCGAGCAAGACCCCTAACCGCCTTATGGCGGTTTCTCTGCCTCCCa                                                                        <  5:274469/148‑1 (MQ=255)
                                                                              ggATAGCCTTAAGGCGTAATTGTCTGATACCAACACCTCTGCGATGAGGACATGACCTCTTCATCGG‑CGCGCCTTAAGCCCGCCGA‑GAAGAGCCAGAGACCCCGAGCAAGACCCCTAACCGCCTTATGGCGGTTTCTCTGCCTCCCAGt                                                                      >  4:212281/1‑149 (MQ=255)
                                                                                aTAGCCTTAAGGCGTAATTGTCTGATACCAACACCTCTGCGATGAGGACATGACCTCTTCATCGG‑CGCGCCTTAAGCCCGCCGA‑GAAGAGCCAGAGACCCCGAGCAAGAc                                                                                                           >  5:152076/1‑110 (MQ=255)
                                                                                aTAGCCTTAAGGCGTAATTGTCTGATACCAACACCTCTGCGATGAGGACATGACCTCTTCATCGG‑CGCGCCTTAAGCCCGCCGA‑GAAGAGCCAGAGACCCCGAGCAAGAc                                                                                                           <  6:152076/110‑1 (MQ=255)
                                                                                aTAGCCTTAAGGCGTAATTGTCTGATACCAACACCTCTGCGATGAGGACATGACCTCTTCATCGG‑CGCGCCTTAAGCCCGCCGA‑GAAGAGCCAGAGACCCCGAGCAAGACCCCTAACCGCCTTATGGCGGTTTCTCTGCCTCCCAGTct                                                                    >  2:217221/1‑149 (MQ=255)
                                                                                 tAGCCTTAAGGCGTAATTGTCTGATACCAACACCTCTGCGATGAGGACATGACCTCTTCATCGG‑CGCGCCTTAAGCCCGCCGA‑GAAGAGCCAGAGACCCCGAGCAAGACCCCTAACCGCCTTATGGCGGTTTctct                                                                                <  4:289286/136‑1 (MQ=255)
                                                                                 tAGCCTTAAGGCGTAATTGTCTGATACCAACACCTCTGCGATGAGGACATGACCTCTTCATCGG‑CGCGCCTTAAGCCCGCCGA‑GAAGAGCCAGAGACCCCGAGCAAGACCCCTAACCGCCTTATGGCGGTTTctct                                                                                >  3:289286/1‑136 (MQ=255)
                                                                                   gCCTTAAGGCGTAATTGTCTGATACCAACACCTCTGCGATGAGGACATGACCTCTTCATCGG‑CGCGCCTTAAGCCCGCCGA‑GAAGAGCCAGAGACCCCGAGCAAGACCCCTAACCGCCTTATGGCGGTTTCTCTGCCTCCCAGTctgct                                                                 <  3:212281/149‑1 (MQ=255)
                                                                                      ttAAGGCGTAATTGTCTGATACCAACACCTCTGCGATGAGGACATGACCTCTTCATCGG‑CGCGCCTTAAGCCCGCCGA‑GAAGAGCCAGAGACCCCGAGCAAGACCCCTAACCGCCTTATGGCGGTTTCTCTGCCTCCCAGTCtgctgc                                                               >  5:147732/1‑148 (MQ=255)
                                                                                         aGGCGTAATTGTCTGATACCAACACCTCTGCGATGAGGACATGACCTCTTCATCGG‑CGCGCCTTAAGCCCGCCGA‑GAAGAGCCAGAGACCCCGAGCAAGACCCCTAACCGCCTTATGGCGGTTTCTCTGCCTCCCAGTCTGCTGCGaa                                                            <  1:217221/148‑1 (MQ=255)
                                                                                             gTAATTGTCTGATACCAACACCTCTGCGATGAGGACATGACCTCTTCATCGG‑CGCGCCTTAAGCCCGCCGA‑GAAGAGCCAGAGACCCCGAGCAAGACCCCTAACCGCCTTATGGCGGTTTCTCTGCCTCCCAGTCTGCTGCGAAGGCCg                                                       <  6:278440/149‑1 (MQ=255)
                                                                                              tAATTGTCTGATACCAACACCTCTGCGATGAGGACATGACCTCTTCATCGG‑CGCGCCTTAAGCCCGCCGA‑GAAGAGCCAGAGACCCCGAGCAAGACCCCTAACCGCCTTATGGCGGTTTCTCTGCCTCCCAGTCTGCTGCGAAGGCCGa                                                      <  4:362680/149‑1 (MQ=255)
                                                                                                aTTGTCTGATACCAACACCTCTGCGATGAGGACATGACCTCTTCATCGG‑CGCGCCTTAAGCCCGCCGA‑GAAGAGCCAGAGACCCCGAGCAAGACCCCTAACCGCCTTATGGCGGTTTCTCTGCCTCCCAGTCTGCTGCGAAGGCCGa                                                      >  1:177550/1‑147 (MQ=255)
                                                                                                aTTGTCTGATACCAACACCTCTGCGATGAGGACATGACCTCTTCATCGG‑CGCGCCTTAAGCCCGCCGA‑GAAGAGCCAGAGACCCCGAGCAAGACCCCTAACCGCCTTATGGCGGTTTCTCTGCCTCCCAGTCTGCTGCGAAGGCCGATa                                                    <  8:6954/149‑1 (MQ=255)
                                                                                                   gTCTGATACCAACACCTCTGCGATGAGGACATGACCTCTTCATCGG‑CGCGCCTTAAGCCCGCCGA‑GAAGAGCCAGAGACCCCGAGCAAGACCCCTAACCGCCTTATGGCGGTTTCTCTGCCTCCCAGTCTGCTGCGAAGGCCGATAGc                                                  <  7:197380/148‑1 (MQ=255)
                                                                                                   gTCTGATACCAACACCTCTGCGATGAGGACATGACCTCTTCATCGG‑CGCGCCTTAAGCCCGCCGA‑GAAGAGCCAGAGACCCCGAGCAAGACCCCTAACCGCCTTATGGCGGTTTCTCTGCCTCCCAGTCTGCTGCGAAGGCCGATAGc                                                  <  5:273753/148‑1 (MQ=255)
                                                                                                    tCTGATACCAACACCTCTGCGATGAGGACATGACCTCTTCATCGG‑CGCGCCTTAAGCCCGCCGA‑GAAGAGCCAGAGACCCCGAGCAAGACCCCTAACCGCCTTATGGCGGTTTCTCTGCCTCCCAGTCTGCTGCGAAGGCCGATAGcc                                                 <  1:307907/148‑1 (MQ=255)
                                                                                                       gATACCAACACCTCTGCGATGAGGACATGACCTCTTCATCGG‑CGCGCCTTAAGCCCGCCGA‑GAAGAGCCAGAGACCCCGAGCAAGACCCCTAACCGCCTTATg                                                                                           >  4:357799/1‑103 (MQ=255)
                                                                                                       gATACCAACACCTCTGCGATGAGGACATGACCTCTTCATCGG‑CGCGCCTTAAGCCCGCCGA‑GAAGAGCCAGAGACCCCGAGCAAGACCCCTAACCGCCTTATg                                                                                           <  3:357799/103‑1 (MQ=255)
                                                                                                        aTACCAACACCTCTGCGATGAGGACATGACCTCTTCATCGG‑CGCGCCTTAAGCCCGCCGA‑GAAGAGCCAGAGACCCCGAGCAAGACCCCTAACCGCCTTATGGCGGTTTCTCTGCCTCCCAGTCTGCTGCGAAGGCCGATAGCCGa                                               <  6:147732/146‑1 (MQ=255)
                                                                                                        aTACCAACACCTCTGCGATGAGGACATGACCTCTTCATCGG‑CGCGCCTTAAGCCCGCCGA‑GAAGAGCCAGAGACCCCGAGCAAGACCCCTAACCGCCTTATGGCGGTTTCTCTGCCTCCCAGTCTGCTGCGAAGGCCGATAGCCGa                                               <  3:198455/146‑1 (MQ=255)
                                                                                                            cAACACCTCTGCGATGAGGACATGACCTCTTCATCGG‑CGCGCCTTAAGCCCGCCGA‑GAAGAGCCAGAGACCCCGAGCAAGACCCCTAACCGCCTTATGGCGGTTTCTCTGCCTCCCAGTCTGCTGCGAAGGCCGATAGCCGAGTccaac                                        >  3:308726/1‑149 (MQ=255)
                                                                                                            cAACACCTCTGCGATGAGGACATGACCTCTTCATCGG‑CGCGCCTTAAGCCCGCCGA‑GAAGAGCCAGAGACCCCGAGCAAGACCCCTAACCGCCTTATGGCGGTTTCTCTGCCTCCCAGTCTGCTGCGAAGGCCGATAGCCGAGTccaac                                        <  6:241560/149‑1 (MQ=255)
                                                                                                                             ggACATGACCTCTTCATCGG‑CGCGCCTTAAGCCCGCCGA‑GAAGAGCCAGAGACCCCGAGCAAGACCCCTAACCGCCTTATGGCGGTTTCTCTGCCTCCCAGTCTGCTGCGAAGGCCGATAGCCGAGTCCAACCAATTCTATTTTTCGAc                       >  7:303126/1‑149 (MQ=255)
                                                                                                                             ggACATGACCTCTTCATCGG‑CGCGCCTTAAGCCCGCCGA‑GAAGAGACAGAGACCCCGAGCAAGACCCCTAACCGCCTTATGGCGGTTTCTCTGCCTCCCAGTCTGCTGCGAAGGCCGATAGCCGAGTCCAACCAATTCTATTTTTCGAc                       >  4:260391/1‑149 (MQ=255)
                                                                                                                              gACATGACCTCTTCATCGG‑CGCGCCTTAAGCCCGCCGA‑GAAGAGCCAGAGACCCCGAGCAAGACCCCTAACCGCCTTATGGCGGTTTCTCTGCCTCCCAGTCTGCTGCGAAGGCCGATAGCCGAGTCCAACCAATTCTATTTTTCGAcc                      >  5:23592/1‑149 (MQ=255)
                                                                                                                              gACATGACCTCTTCATCGG‑CGCGACTTAAGCCAGCCGA‑GAACAGCGAGAGACACCGAGCAAGCCCCCTAACCGCCATATGGCGGTTTCTCTTCCTCCAAGTATGCTGGGACTGCAGATATCCGAGTCCCCCAAATGCTATTTTTAGAcc                      >  4:184369/1‑149 (MQ=255)
                                                                                                                                cATGACCTCTTCATCGG‑CGCGCCTTAAGCCCGCCGA‑GAAGAGCCAGAGACCCCGAGCAAGACCCCTAACCGCCTTATGGCGGTTTCTCTGCCTCCCAGTCTGCTGCGAAGGCCGATAGCCGAGTCCAACCAATTCTATTTTTCGACCTg                    <  6:23592/149‑1 (MQ=255)
                                                                                                                                 aTGACCTCTTCATCGG‑CGCGCCTTAAGCCCGCCGA‑GAAGAGCCAGAGACCCCGAGCAAGAc                                                                                                           <  6:281957/61‑1 (MQ=255)
                                                                                                                                 aTGACCTCTTCATCGG‑CGCGCCTTAAGCCCGCCGA‑GAAGAGCCAGAGACCCCGAGCAAGAc                                                                                                           >  5:281957/1‑61 (MQ=255)
                                                                                                                                 aTGACCTCTTCATCGG‑CGCGCCTTAAGCCCGCCGA‑GAAGAGCCAGAGACCCCGAGCAAGACCCCTAACCGCCTTATGGCGGTTTCTCTGCCTCCCAGTCTGCTGCGAAGGCCGATAGCCGAGTCCAACCAATTCTATTTTTCGACCTgg                   >  7:21549/1‑149 (MQ=255)
                                                                                                                                 aTGACCTCTTCATCGG‑CGCGCCTTAAGCCCGCCGA‑GAAGAGCCAGACACCCCGAGCAAGACCCCTAACCGCCTTATGGCCGTTTCTCTGCCTCCCCCTCTGCTGCGAAGGCCGAGAGCCGACTCCAACCAAATCTAATTTTCGACCTgg                   >  7:72814/1‑149 (MQ=255)
                                                                                                                                      ctctTCATCGG‑CGCGCCTTAAGCCCGCCGA‑GAAGAGCCAGAGACCCCGAGCAAGACCCCTAACCTCCTTATGGCGGTTTCTCTGCCTCCCAGTCTGCTGCGAAGGCCGATATCCGAGTCCAACCAATTCTATTTTTCGACCTGGCCTGa              <  6:371603/149‑1 (MQ=255)
                                                                                                                                      ctctTCATCGG‑CGCGCCTTAAGCCCGCCGA‑GAAGAGCCAGAGACCCCGAGCAAGACCCCTAACCGCCTTATGGCGGTTTCTCTGCCTCCCAGTCTGCTGCGAAGGCCGATAGCCGAGTCCAACCAATTCTATTTTTCGACCTGGCCt                <  4:268968/147‑1 (MQ=255)
                                                                                                                                      ctctTCATCGG‑CGCGCCTTAAGCCCGCCGA‑GAAGAGCCAGAGACCCCGAGCAAGACCCCTAACCGCCTTATGGCGGTTTCTCTGCCTCCCAGTCTGCTGCGAAGGCCGATAGCCGAGTCCAACCAATTCTATTTTTCGACCTGGCCt                >  3:268968/1‑147 (MQ=255)
                                                                                                                                       tctTCATCGG‑CGCGCCTTAAGCCCGCCGA‑GAAGAGCCAGAGACCCCGAGCAAGACCACTAACAGCCTTATGGCGGTTTCTCTGCCTCCCAGTCTGCTGCGAAGGCCGATAGCCGAGTCCAACCAATTATATTTATCGACCAGGCCTGa              >  4:26935/1‑148 (MQ=255)
                                                                                                                                         ttgaTCGG‑CTCGCATTAAGCCCGCCGA‑GAATAGCCAGAGACCGCGAGCAAGACCCCTAACCGCCTTATGGCGGTTTCTCTGCCTCCCATTCTGCTGCGTATGCCGATAGCCGAGTCCAACCAATTCTATTTTTCGACCTGGCCTGAc             <  2:184448/144‑1 (MQ=255)
                                                                                                                                             tCGG‑CGGGCTTAAGGCGCGCCGATGAAGAGCCAGAGACCCCGAGCAAGACCCCTAACCGCCTTAt                                                                                            >  7:340932/1‑65 (MQ=18)
                                                                                                                                             tCGG‑CGGGCTTAAGGCGCGCCGATGAAGAGCCAGAGACCCCGAGCAAGACCCCTAACCGCCTTAt                                                                                            <  8:340932/65‑1 (MQ=18)
                                                                                                                                             tCGG‑CGCGCCTTAAGCCCGCCGA‑GAAGAGCCAGAGACCCCGAGCAAGACCCCTAACCGCCTTATGGCGGTTTCTCTGCCTCCCAGTCTGCTGCGAAGGCCGATAGCCGAGTCAAACCAATTCTATTTTTCGACCTGGCCTGACCGCAg        >  4:290801/1‑148 (MQ=255)
                                                                                                                                              cgg‑cggGCTTAAGGCGCGCCGA‑GAAGAGCCAGAGACCCCGAGCAAGACCCCTAACCGCCTTATGGCGGTTTCTCTGCCTCCCAGTCTGCTGCGAAGGCCGATAGCCGAGTCCAACCAATTCTATTTTTCGACCTGGCCTGACCGCAg        >  8:30875/1‑147 (MQ=255)
                                                                                                                                               gg‑CGCGCCTTAAGCCCGCCGA‑GAAGAGCCAGAGACCCCGAGCAAGACCCCTAACCGCCTTATGGCGGTTTCTCTGCCTCCCAGTCTGCTGCGAAGGCCGATAGCCGAGTCCAACCAATTCTATTTTTCGACCTGGCCTGACCGCAGTa      <  7:281611/148‑1 (MQ=255)
                                                                                                                                               gg‑CGCGCCTTAAGCCCGCCGA‑GAAGAGCCAGAGACCCCGAGCAAGACCCCTAACCGCCTTATGGCGGTTTCTCTGCCTCCCAGTCTGCTGCGAAGGCCGATAGCCGAGTCCAACCAATTCTATTTTTCGACCTGGCCTGACCGCAGTa      <  2:135736/148‑1 (MQ=255)
                                                                                                                                                g‑cgtgcCTTAAGCCCTCAGA‑GAAGATCCTGAGACCCCTAGCAAGACCCCTAACCGCCTTATGGCTGTTTCTCTGCCTCCCAGTCTGCTGCGAAGGCCGATAGCCGAGTCCAACCAATTGTATTTTTCGACCTGGCCTGACCGCAGTaga    <  7:344506/149‑1 (MQ=255)
                                                                                                                                                   gcgcCTTAAGCCCGCCGA‑GAAGAGCCAGAGACCCCGAGCAAGACCCCTAACCGCCTTATGGCGGTTTCTCTGCCTCCCAGTCTGCTGCGAAGGCCGATAGCCGAGTCCAACCAATTCTATTTTTCGACCTGGCCTGACCGCAGTAGAGc  >  7:22694/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
ACACTTGTACTCCACACCCAGTCTCACATCTGACCCTGTCAGCGACGCTAATGACGGTGTTGATCCACAGAGGGTTAGGGATAGCCTTAAGGCGTAATTGTCTGATACCAACACCTCTGCGATGAGGACATGACCTCTTCATCGG‑CGCGCCTTAAGCCCGCCGA‑GAAGAGCCAGAGACCCCGAGCAAGACCCCTAACCGCCTTATGGCGGTTTCTCTGCCTCCCAGTCTGCTGCGAAGGCCGATAGCCGAGTCCAACCAATTCTATTTTTCGACCTGGCCTGACCGCAGTAGAGC  >  NC_002947/5041048‑5041342

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑