Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,041,200 T→A 7.7% intergenic (+178/‑484) PP_5680 → / → PP_4447 Cro/CI family transcriptional regulator/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,041,2000TA 7.7% 267.8 / 8.9 91intergenic (+178/‑484)PP_5680/PP_4447Cro/CI family transcriptional regulator/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (41/43);  new base A (3/4);  total (44/47)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.62e-01

GTACTCCACACCCAGTCTCACATCTGACCCTGTCAGCGACGCTAATGACGGTGTTGATCCACAGAGGGTTAGGGATAGCCTTAAGGCGTAATTGTCTGATACCAACACCTCTGCGATGAGGACATGACCTCTTCATCGG‑CGCGCCTTAAGCCCGCCGA‑GAAGAGCCAGAGACCCCGAGCAAGACCCCTAACCGCCTTATGGCGGTTTCTCTGCCTCCCAGTCTGCTGCGAAGGCCGATAGCCGAGTCCAACCAATTCTATTTTTCGACCTGGCCTGACCGCAGTAGAGCGT  >  NC_002947/5041054‑5041344
                                                                                                                                                   |                                                                                                                                                 
gTACTCCACACCCAGTCTCACATCTGACCCTGTCAGCGACGCTAATGACGGTGTTGATCCACAGAGGGTTAGGGATAGCCTTAAGGCGTAATTGTCTGATACCAACACCTCTGCGATGAGGACATGACCTCTTCATCGG‑CGCGCCtt                                                                                                                                                   >  1:276225/1‑147 (MQ=255)
      cacaCCCAGTCTCACATCTGACCCTGTCAGCGACGCTAATGACGGTGTTGATCCACAGAGGGTTAGGGATAGCCTTAAGGCGTAATTGTCTGATACCAACACCTCTGCGATGAGGACATGACCTCTTCATCGG‑CGCGCCTTAAGCccgc                                                                                                                                           >  6:89605/1‑149 (MQ=255)
          cccAGTCTCACATCTGACCCTGTCAGCGACGCTAATGACGGTGTTGATCCACAGAGGGTTAGGGATAGCCTTAAGGCGTAATTGTCTGATACCAACACCTCTGCGATGAGGACATGACCTCTTCATCGG‑CGCGCCTTAAGCccgcc                                                                                                                                          >  5:70333/1‑146 (MQ=255)
          cccAGTCTCACATCTGACCCTGTCAGCGACGCTAATGACGGTGTTGATCCACAGAGGGTTAGGGATAGCCTTAAGGCGTAATTGTCTGATACCAACACCTCTGCGATGAGGACATGACCTCTTCATCGG‑CGCGCCTTAAGCccgcc                                                                                                                                          <  6:70333/146‑1 (MQ=255)
             aGTCTCACATCTGACCCTGTCAGCGACGCTAATGACGGTGTTGATCCACAGAGGGTTAGGGATAGCCTTAAGGCGTAATTGTCTGATACCAACACCTCTGCGATGAGGACATGACCTCTTCATCGG‑CGGGCTTAAGGCGCGCCgatgaa                                                                                                                                    <  8:183197/149‑5 (MQ=255)
              gTCTCACATCTGACCCTGTCAGCGACGCTAATGACGGTGTTGATCCACAGAGGGTTAGGGATAGCCTTAAGGCGTAATTGTCTGATACCAACACCTCTGCGATGAGGACATGACCTCTTCATCGG‑CGCGCCTTAAGCCCGCCGa‑gaaga                                                                                                                                  <  2:276225/149‑1 (MQ=255)
              gTCTCACATCTGACCCTGTCAGCGACGCTAATGACGGTGTTGATCCACAGAGGGTTAGGGATAGCCTTAAGGCGTAATTGTCTGATACCAACACCTCTGCGATGAGGACATGACCTCTTCATCGG‑CGCGCCTTAAGCCCGCCGa‑gaaga                                                                                                                                  <  8:143623/149‑1 (MQ=255)
              gTCTCACATCTGACCCTGTCAGCGACGCTAATGACGGTGTTGATCCACAGAGGGTTAGGGATAGCCTTAAGGCGTAATTGTCTGATACCAACACCTCTGCGATGAGGACATGACCTCTTC‑TCGG‑CGGGCTTAAGGCGCGCCGATgaaga                                                                                                                                  <  3:149848/149‑1 (MQ=38)
               tctcACATCTGACCCTGTCAGCGACGCTAATGACGGTGTTGATCCACAGAGGGTTAGGGATAGCCTTAAGGCGTAATTGTCTGATACCAACACCTCTGCGATGAGGACATGACCTCTTCATCGG‑CGCGCCTTAAGCCCGCCGA‑GAagag                                                                                                                                 >  8:197380/1‑149 (MQ=255)
                 tcACATCTGACCCTGTCAGCGACGGTAATGACGGTGTTGATCCACAGAGGGTTAGGGATAGCCTTAAGGCGTAATTGTCTGATACCAACACCTCTGCGATGAGGACATGACCTCTTCATCGG‑CGCGCCTTAAGCCCGCCGA‑GAAGAGc                                                                                                                                >  7:52526/1‑148 (MQ=255)
                 tcACATCTGACCCTGTCAGCGACGCTAATGACGGTGTTGATCCACAGAGGGTTAGGGATAGCCTTAAGGCGTAATTGTCTGATACCAACACCTCTGCGATGAGGACATGACCTCTTCATCGG‑CGCGCCTTAAGCCCGCCGA‑GAataga                                                                                                                                >  8:146935/1‑147 (MQ=255)
                  cacaTCTGACCCTGTCAGCGACGCTAATGACGGTGTTGATCCACAGAGGGTTAGGGATAGCCTTAAGGCGTAATTGTCTGATACCAACACCTCTGCGATGAGGACATGACCTCTTCATCGG‑CGCGCCTTAAGCCCGCCGA‑GAAGAGc                                                                                                                                >  5:241560/1‑147 (MQ=255)
                   acaTCTGACCCTGTCAGCGACGCTAATGACGGTGTTGATCCACAGAGGGTTAGGGATAGCCTTAAGGCGTAATTGTCTGATACCAACACCTCTGCGATGAGGACATGACCTCTTCATCGGCCGCGCCTTAAGCCCGCCGA‑GAAGAGCCa                                                                                                                              <  1:205182/149‑1 (MQ=255)
                   acaTCTGACCCTGTCAGCGACGCTAATGACGGTGTTGATCCACAGAGGGTTAGGGATAGCCTTAAGGCGTAATTGTCTGATACCAACACCTCTGCGATGAGGACATGACCTCTTCATCGG‑CGCGCCTTAAGCCCGCCGA‑GAAGAGc                                                                                                                                >  6:139066/1‑146 (MQ=255)
                   acaTCTGACCCTGTCAGCGACGCTAATGACGGTGTTGATCCACAGAGGGTTAGGGATAGCCTTAAGGCGTAATTGTCTGATACCAACACCTCTGCGATGAGGACATGACCTCTTCATCGG‑CGCGCCTTAAGCCCGCCGA‑GAAGAGCCa                                                                                                                              <  8:52526/148‑1 (MQ=255)
                   aaaTCTGACCCTGTCAGCGACCCTAATGACGGTGTTGATCCAGAGAGGGTTAGGGAAATCCTTAAGGCTTAATTGTCTGATACCAACTCCTCTGCGATGATTACATGACATCTTCATCGT‑CGGGCCTTAAGCCCTCAGA‑GAATAGCCag                                                                                                                             <  8:341501/147‑1 (MQ=255)
                    caTCTGACCCTGTCAGCGACGCTAATGACGGTGTTGATCCACAGAGGGTTAGGGATAGCCTTAAGGCGTAATTGTCTGATACCAACACCTCTGCGATGAGGACATGACCTCTTC‑TCGG‑CGGGCTTAAGGCGCGCCgatgaa                                                                                                                                    <  1:334048/141‑5 (MQ=255)
                    caTCTGACCCTGTCAGCGACGCTAATGACGGTGTTGATCCACAGAGGGTTAGGGATAGCCTTAAGGCGTAATTGTCTGATACCAACACCTCTGCGATGAGGACATGACCTCTTC‑TCGG‑CGGGCTTAAGGCGCGCCgatgaa                                                                                                                                    >  2:334048/1‑137 (MQ=255)
                     aTCTGACCCTGTCAGCGACGCTAATGACGGTGTTGATCCACAGAGGGTTAGGGATAGCCTTAAGGCGTAATTGTCTGATACCAACACCTCTGCGATGAGGACATGACCTCTTCATCGG‑CGCGCCTTAAGCCCGCCGA‑GAAGAGCCagag                                                                                                                           >  8:276134/1‑149 (MQ=255)
                          aCCCTGTCAGCGACGCTAATGACGGTGTTGATCCACAGAGGGTTAGGGATAGCCTTAAGGCGTAATTGTCTGATACCAACACCTCTGCGATGAGGACATGACCTCTTCATCGG‑CGCGCCTTAAGCCCGCCGA‑GAAGAGCCAGAGAcccc                                                                                                                      >  1:305695/1‑149 (MQ=255)
                            ccTGTCAGCGACGCTAATGACGGTGTTGATCCACAGAGGGTTAGGGATAGCCTTAAGGCGTAATTGTCTGATACCAACACCTCTGCGATGAGGACATGACCTCTTCTTCGG‑CGCGCCTTAAGCCCGCCGA‑GAAGAGCCAGAGACCCCGa                                                                                                                    <  1:256903/149‑1 (MQ=255)
                            ccTGTCAGCGACGCTAATGACGGTGTTGATCCACAGAGGGTTAGGGATAGCCTTAAGGCGTAATTGTCTGATACCAACACCTCTGCGATGAGGACATGACCTCTTCATCGG‑CGCGCCTTaa                                                                                                                                                 <  1:294694/121‑1 (MQ=255)
                            ccTGTCAGCGACGCTAATGACGGTGTTGATCCACAGAGGGTTAGGGATAGCCTTAAGGCGTAATTGTCTGATACCAACACCTCTGCGATGAGGACATGACCTCTTCATCGG‑CGCGCCTTaa                                                                                                                                                 >  2:294694/1‑121 (MQ=255)
                            ccTGTCAGCGACGCTAATGACGGTGTTGATCCACAGAGGGTTAGGGATAGCCTTAAGGCGTAATTGTCTGATACCAACACCTCTGCGATGAGGACATGACCTCTTCATCGG‑CGCGCCTTAAGCCCGCCGA‑GAAGAGCCAGAGACCCCGa                                                                                                                    >  2:116590/1‑149 (MQ=255)
                            ccTGTCAGCGACGCTAATGACGGTATTGATCCACAGAGGGTTTGGGAAAGCCTTAAGGCGTAATTTTCTGATACCAACACCTCTGGGATGAGGACATGACCTCTTCAACCG‑CGCGCCTTAAGCCCGCCGA‑GAAAAGCCAGAGCcccccc                                                                                                                    >  7:52639/1‑147 (MQ=255)
                             cTGTCAGCGACGCTAATGACGGTGTTGATCCACAGAGGGTTAGGGATAGCCTTAAGGCGTAATTGTCTGATACCAACACCTCTGCGATGAGGACATGACCTCTTCATCGG‑CGCGCCTTAAGCCCGCCGA‑GAAGAGCCAGAGACCCCGAg                                                                                                                   <  1:33976/149‑1 (MQ=255)
                                 cAGCGACGCTAATGACGGTGTTGATCCACAGAGGGTTAGGGATAGCCTTAAGGCGTAATTGTCTGATACCAACACCTCTGCGATGAGGACATGACCTCTTCATCGG‑CGCGCCTTAAGACAGCCGA‑GAAGAGCCAGAGACCCCGAGc                                                                                                                  >  8:307353/1‑146 (MQ=255)
                                  aGCGACGCTAATGACGGTGTTGATCCACAGAGGGTTAGGGATAGCCTTAAGGCGTAATTGTCTGATACCAACACCTCTGCGATGAGGACATGACCTCTTCATCGG‑CGCGCCTTAAGCCCGCCGA‑GAAGAGCCAGAGACCCCGAGCAAGa                                                                                                              <  2:305695/149‑1 (MQ=255)
                                   gCGACGCTAATGACGGTGTTGATCCACAGAGGGTTAGGGATAGCCTTAAGGCGTAATTGTCTGATACCAACACCTCTGCGATGAGGACATGACCTCTTCATCGG‑CGCGCCTTAAGCCCGCCGA‑GAAGAGCCAGAGACCCCGAGCAAGAc                                                                                                             <  5:89605/149‑1 (MQ=255)
                                   gCGACGCTAATGACGGTGTTGATCCACAGAGGGTTAGGGATAGCCTTAAGGCGTAATTGTCTGATACCAACACCTCTGCGATGAGGACATGACCTCTTCATCGG‑CGCGCCTTAAGCCCGCCGA‑GAAGAGCCAGAGACCCCGAGCAAGAc                                                                                                             >  1:135736/1‑149 (MQ=255)
                                     gACGCTAATGACGGTGTTGATCCACAGAGGGTTAGGGATAGCCTTAAGGCGTAATTGTCTGATACCAACACCTCTGCGATGAGGACATGACCTCTTCATCGG‑CGCGCCTTAAGCCCGCCGA‑GAAGAGCCAGAGACCCCGAGCAAGAccc                                                                                                           <  6:155051/149‑1 (MQ=255)
                                      aCGCTAATGACGGTGTTGATCCACAGAGGGTTAGGGATAGCCTTAAGGCGTAATTGTCTGATACCAACACCTCTGCGATGAGGACATGACCTCTTCATCGG‑CGCGCCTTAAGCCCGCCGA‑GAAGAGCCAGAGACCCCGAGCAAGAcccc                                                                                                          <  3:170863/149‑1 (MQ=255)
                                          tAATGACGGTGTTGATCCACAGAGGGTTAGGGATAGCCTTAAGGCGTAATTGTCTGATACCAACACCTCTGCGATGAGGACATGACCTCTTCATCGG‑CGCGCCTTAAGCCCGCCGA‑GAAGAGCCAGAGACCCCGAGCAAGACCCCTAAc                                                                                                      >  3:362680/1‑149 (MQ=255)
                                          tAATGACGGTGTTGATCCACAGAGGGTTAGGGATAGCCTTAAGGCGTAATTGTCTGATACCAACACCTCTGCGATGAGGACATGACCTCTTCATCGG‑CGCGCCTTAAGCCCGCCGA‑GAAGAGCCAGAGACCCCGAGCAAGACCCCTAAc                                                                                                      >  2:307907/1‑149 (MQ=255)
                                          tAATGACGGTGTTGATCCACAGAGGGTTAGGGATAGCCTTAAGGCGTAATTGTCTGATACCAACACCTCTGCGATGAGGACATGACCTCTTAATCGG‑CGCGCCTTAAGCCCGCCGA‑GAAGAGCCAGAGACCCCGAGCAACACACCTAAc                                                                                                      >  1:184448/1‑149 (MQ=255)
                                              gACGGTGTTGATCCACAGAGGGTTAGGGATAGCCTTAAGGCGTAATTGTCTGATACCAACACCTCTGCGATGAGGACATGACCTCTTCATCGG‑CGCGCCTTAAGCCCGCCGA‑GAAGAGCCAGAGACCCCGAGCAAGACCCCTAACCGcc                                                                                                  >  6:274469/1‑149 (MQ=255)
                                               aCGGTGTTGATCCACAGAGGGTTAGGGATAGCCTTAAGGCGTAATTGTCTGATACCAACACCTCTGCGATGAGGACATGACCTCTTCATCGG‑CGCGCCTTAAGCCCGCCGA‑GAAGAGCCAGAGACCCCGAGCAAGACCCCTAACCGCCt                                                                                                 >  4:198455/1‑149 (MQ=255)
                                               aCGGTGTTGATCCACAGAGGGTTAGGGATAGCCTTAAGGCGTAATTGTCTGATACCAACACCTCTGCGATGAGGACATGACCTCTTCATCGG‑CGCGCCTTAAGCCCGCCGA‑GAAGAGCCAGAGACCCCGAGCAAGACCCCTAACCGCCt                                                                                                 >  6:273753/1‑149 (MQ=255)
                                                 ggTGTTGATCCACAGAGGGTTAGGGATAGCCTTAAGGCGTAATTGTCTGATACCCACACCTCTGCGATGAGGACATGACCTCTTCATCGG‑CGCGCCTTAAGCCCGCCGA‑GAAGAGCCAGAGACCCCGAGCAAGACCCCTAACCGCCTTa                                                                                               >  8:281611/1‑149 (MQ=255)
                                                         tCCACAGAGGGTTAGGGATAGCCTTAAGGCGTAATTGTCTGATACCAACACCTCTGCGATGAGGACATGACCTCTTCATCGG‑CGCGCCTTAAGCCCGCCGA‑GAAGAGCCAGAGACCCCGAGCAAGACCCCTAACCGCCATAAGGCGGt                                                                                        <  3:66048/148‑1 (MQ=255)
                                                           cacaGAGGGTTAGGGATAGCCTTAAGGCGTAATTGTCTGATACCAACACCTCTGCGATGAGGACATGACCTCTTCATCGG‑CGCGCCTTAAGCCCGCCGA‑GAAGAGCCAGAGACCCCGAGCAAGACCCCTAACCGCCTTATGGCGGTTtc                                                                                     >  7:6954/1‑149 (MQ=255)
                                                            acaGAGGGTTAGGGATAGCCTTAAGGCGTAATTGTCTGATACCAACACCTCTGCGATGAGGACATGACCTCTTCATCGG‑CGCGCCTTAAGCCCGCCGA‑GAAGAGCCAGAGACCCCGAGCAAGACCCCTAACCGCCTTATGGCGGTTtct                                                                                    <  5:8284/149‑1 (MQ=255)
                                                             caGAGGGTTAGGGATAGCCTTAAGGCGTAATTGTCTGATACCAACACCTCTGCGATGAGGACATGACCTCTTCATCGG‑CGCGCCTTAAGCCCGCCGA‑GAAGAGCCAGAGACCCCGAGCAAGACCCCTAACCGCCTTATGGCGGTTtctc                                                                                   <  3:125378/149‑1 (MQ=255)
                                                              agagGGTTAGGGATAGCCTTAAGGCGTAATTGTCTGATACCAACACCTCTGCGATGAGGACATGACCTCTTCATCGG‑CGCGCCTTAAGCCCGCCGA‑GAAGAGCCAGAGACCCCGAGCAAGACCCCTAACCGCCTTATGGCGGTTTctct                                                                                  <  7:276134/149‑1 (MQ=255)
                                                                  ggTTAGGGATAGCCTTAAGGCGTAATTGTCTGATACCAACACCTCTGCGATGAGGACATGACCTCTTCATCGG‑CGCGCCTTAAGCCCGCCGA‑GAAGAGCCAGAGACCCCGAGCAAGACCCCTAACCGCCTTATGGCGGTTTCTCTGCCt                                                                              <  7:146935/149‑1 (MQ=255)
                                                                    ttAGGGATAGCCTTAAGGCGTAATTGTCTGATACCAACACCTCTGCGATGAGGACATGACCTCTTCATCGG‑CGCGCCTTAAGCCCGCCGA‑GAAGAGCCAGAGACCCCGAGCAAGACCCCTAACCGCCTTATGGCGGTTTCTCTGCCTcc                                                                            <  1:116590/149‑1 (MQ=255)
                                                                       gggATAGCCTTAAGGCGTAATTGTCTGATACCAACACCTCTGCGATGAGGACATGACCTCTTCATCGG‑CGCGCCTTAAGCCCGCCGA‑GAAGAGCCAGAGACCCCGAGCAAGACCCCTAACCGCCTTATGGCGGTTTCTCTGCCTCCCa                                                                          <  5:274469/148‑1 (MQ=255)
                                                                        ggATAGCCTTAAGGCGTAATTGTCTGATACCAACACCTCTGCGATGAGGACATGACCTCTTCATCGG‑CGCGCCTTAAGCCCGCCGA‑GAAGAGCCAGAGACCCCGAGCAAGACCCCTAACCGCCTTATGGCGGTTTCTCTGCCTCCCAGt                                                                        >  4:212281/1‑149 (MQ=255)
                                                                          aTAGCCTTAAGGCGTAATTGTCTGATACCAACACCTCTGCGATGAGGACATGACCTCTTCATCGG‑CGCGCCTTAAGCCCGCCGA‑GAAGAGCCAGAGACCCCGAGCAAGAc                                                                                                             >  5:152076/1‑110 (MQ=255)
                                                                          aTAGCCTTAAGGCGTAATTGTCTGATACCAACACCTCTGCGATGAGGACATGACCTCTTCATCGG‑CGCGCCTTAAGCCCGCCGA‑GAAGAGCCAGAGACCCCGAGCAAGAc                                                                                                             <  6:152076/110‑1 (MQ=255)
                                                                          aTAGCCTTAAGGCGTAATTGTCTGATACCAACACCTCTGCGATGAGGACATGACCTCTTCATCGG‑CGCGCCTTAAGCCCGCCGA‑GAAGAGCCAGAGACCCCGAGCAAGACCCCTAACCGCCTTATGGCGGTTTCTCTGCCTCCCAGTct                                                                      >  2:217221/1‑149 (MQ=255)
                                                                           tAGCCTTAAGGCGTAATTGTCTGATACCAACACCTCTGCGATGAGGACATGACCTCTTCATCGG‑CGCGCCTTAAGCCCGCCGA‑GAAGAGCCAGAGACCCCGAGCAAGACCCCTAACCGCCTTATGGCGGTTTctct                                                                                  >  3:289286/1‑136 (MQ=255)
                                                                           tAGCCTTAAGGCGTAATTGTCTGATACCAACACCTCTGCGATGAGGACATGACCTCTTCATCGG‑CGCGCCTTAAGCCCGCCGA‑GAAGAGCCAGAGACCCCGAGCAAGACCCCTAACCGCCTTATGGCGGTTTctct                                                                                  <  4:289286/136‑1 (MQ=255)
                                                                             gCCTTAAGGCGTAATTGTCTGATACCAACACCTCTGCGATGAGGACATGACCTCTTCATCGG‑CGCGCCTTAAGCCCGCCGA‑GAAGAGCCAGAGACCCCGAGCAAGACCCCTAACCGCCTTATGGCGGTTTCTCTGCCTCCCAGTctgct                                                                   <  3:212281/149‑1 (MQ=255)
                                                                                ttAAGGCGTAATTGTCTGATACCAACACCTCTGCGATGAGGACATGACCTCTTCATCGG‑CGCGCCTTAAGCCCGCCGA‑GAAGAGCCAGAGACCCCGAGCAAGACCCCTAACCGCCTTATGGCGGTTTCTCTGCCTCCCAGTCtgctgc                                                                 >  5:147732/1‑148 (MQ=255)
                                                                                   aGGCGTAATTGTCTGATACCAACACCTCTGCGATGAGGACATGACCTCTTCATCGG‑CGCGCCTTAAGCCCGCCGA‑GAAGAGCCAGAGACCCCGAGCAAGACCCCTAACCGCCTTATGGCGGTTTCTCTGCCTCCCAGTCTGCTGCGaa                                                              <  1:217221/148‑1 (MQ=255)
                                                                                       gTAATTGTCTGATACCAACACCTCTGCGATGAGGACATGACCTCTTCATCGG‑CGCGCCTTAAGCCCGCCGA‑GAAGAGCCAGAGACCCCGAGCAAGACCCCTAACCGCCTTATGGCGGTTTCTCTGCCTCCCAGTCTGCTGCGAAGGCCg                                                         <  6:278440/149‑1 (MQ=255)
                                                                                        tAATTGTCTGATACCAACACCTCTGCGATGAGGACATGACCTCTTCATCGG‑CGCGCCTTAAGCCCGCCGA‑GAAGAGCCAGAGACCCCGAGCAAGACCCCTAACCGCCTTATGGCGGTTTCTCTGCCTCCCAGTCTGCTGCGAAGGCCGa                                                        <  4:362680/149‑1 (MQ=255)
                                                                                          aTTGTCTGATACCAACACCTCTGCGATGAGGACATGACCTCTTCATCGG‑CGCGCCTTAAGCCCGCCGA‑GAAGAGCCAGAGACCCCGAGCAAGACCCCTAACCGCCTTATGGCGGTTTCTCTGCCTCCCAGTCTGCTGCGAAGGCCGa                                                        >  1:177550/1‑147 (MQ=255)
                                                                                          aTTGTCTGATACCAACACCTCTGCGATGAGGACATGACCTCTTCATCGG‑CGCGCCTTAAGCCCGCCGA‑GAAGAGCCAGAGACCCCGAGCAAGACCCCTAACCGCCTTATGGCGGTTTCTCTGCCTCCCAGTCTGCTGCGAAGGCCGATa                                                      <  8:6954/149‑1 (MQ=255)
                                                                                             gTCTGATACCAACACCTCTGCGATGAGGACATGACCTCTTCATCGG‑CGCGCCTTAAGCCCGCCGA‑GAAGAGCCAGAGACCCCGAGCAAGACCCCTAACCGCCTTATGGCGGTTTCTCTGCCTCCCAGTCTGCTGCGAAGGCCGATAGc                                                    <  5:273753/148‑1 (MQ=255)
                                                                                             gTCTGATACCAACACCTCTGCGATGAGGACATGACCTCTTCATCGG‑CGCGCCTTAAGCCCGCCGA‑GAAGAGCCAGAGACCCCGAGCAAGACCCCTAACCGCCTTATGGCGGTTTCTCTGCCTCCCAGTCTGCTGCGAAGGCCGATAGc                                                    <  7:197380/148‑1 (MQ=255)
                                                                                              tCTGATACCAACACCTCTGCGATGAGGACATGACCTCTTCATCGG‑CGCGCCTTAAGCCCGCCGA‑GAAGAGCCAGAGACCCCGAGCAAGACCCCTAACCGCCTTATGGCGGTTTCTCTGCCTCCCAGTCTGCTGCGAAGGCCGATAGcc                                                   <  1:307907/148‑1 (MQ=255)
                                                                                                 gATACCAACACCTCTGCGATGAGGACATGACCTCTTCATCGG‑CGCGCCTTAAGCCCGCCGA‑GAAGAGCCAGAGACCCCGAGCAAGACCCCTAACCGCCTTATg                                                                                             <  3:357799/103‑1 (MQ=255)
                                                                                                 gATACCAACACCTCTGCGATGAGGACATGACCTCTTCATCGG‑CGCGCCTTAAGCCCGCCGA‑GAAGAGCCAGAGACCCCGAGCAAGACCCCTAACCGCCTTATg                                                                                             >  4:357799/1‑103 (MQ=255)
                                                                                                  aTACCAACACCTCTGCGATGAGGACATGACCTCTTCATCGG‑CGCGCCTTAAGCCCGCCGA‑GAAGAGCCAGAGACCCCGAGCAAGACCCCTAACCGCCTTATGGCGGTTTCTCTGCCTCCCAGTCTGCTGCGAAGGCCGATAGCCGa                                                 <  6:147732/146‑1 (MQ=255)
                                                                                                  aTACCAACACCTCTGCGATGAGGACATGACCTCTTCATCGG‑CGCGCCTTAAGCCCGCCGA‑GAAGAGCCAGAGACCCCGAGCAAGACCCCTAACCGCCTTATGGCGGTTTCTCTGCCTCCCAGTCTGCTGCGAAGGCCGATAGCCGa                                                 <  3:198455/146‑1 (MQ=255)
                                                                                                      cAACACCTCTGCGATGAGGACATGACCTCTTCATCGG‑CGCGCCTTAAGCCCGCCGA‑GAAGAGCCAGAGACCCCGAGCAAGACCCCTAACCGCCTTATGGCGGTTTCTCTGCCTCCCAGTCTGCTGCGAAGGCCGATAGCCGAGTccaac                                          <  6:241560/149‑1 (MQ=255)
                                                                                                      cAACACCTCTGCGATGAGGACATGACCTCTTCATCGG‑CGCGCCTTAAGCCCGCCGA‑GAAGAGCCAGAGACCCCGAGCAAGACCCCTAACCGCCTTATGGCGGTTTCTCTGCCTCCCAGTCTGCTGCGAAGGCCGATAGCCGAGTccaac                                          >  3:308726/1‑149 (MQ=255)
                                                                                                                       ggACATGACCTCTTCATCGG‑CGCGCCTTAAGCCCGCCGA‑GAAGAGCCAGAGACCCCGAGCAAGACCCCTAACCGCCTTATGGCGGTTTCTCTGCCTCCCAGTCTGCTGCGAAGGCCGATAGCCGAGTCCAACCAATTCTATTTTTCGAc                         >  7:303126/1‑149 (MQ=255)
                                                                                                                       ggACATGACCTCTTCATCGG‑CGCGCCTTAAGCCCGCCGA‑GAAGAGACAGAGACCCCGAGCAAGACCCCTAACCGCCTTATGGCGGTTTCTCTGCCTCCCAGTCTGCTGCGAAGGCCGATAGCCGAGTCCAACCAATTCTATTTTTCGAc                         >  4:260391/1‑149 (MQ=255)
                                                                                                                        gACATGACCTCTTCATCGG‑CGCGCCTTAAGCCCGCCGA‑GAAGAGCCAGAGACCCCGAGCAAGACCCCTAACCGCCTTATGGCGGTTTCTCTGCCTCCCAGTCTGCTGCGAAGGCCGATAGCCGAGTCCAACCAATTCTATTTTTCGAcc                        >  5:23592/1‑149 (MQ=255)
                                                                                                                        gACATGACCTCTTCATCGG‑CGCGACTTAAGCCAGCCGA‑GAACAGCGAGAGACACCGAGCAAGCCCCCTAACCGCCATATGGCGGTTTCTCTTCCTCCAAGTATGCTGGGACTGCAGATATCCGAGTCCCCCAAATGCTATTTTTAGAcc                        >  4:184369/1‑149 (MQ=255)
                                                                                                                          cATGACCTCTTCATCGG‑CGCGCCTTAAGCCCGCCGA‑GAAGAGCCAGAGACCCCGAGCAAGACCCCTAACCGCCTTATGGCGGTTTCTCTGCCTCCCAGTCTGCTGCGAAGGCCGATAGCCGAGTCCAACCAATTCTATTTTTCGACCTg                      <  6:23592/149‑1 (MQ=255)
                                                                                                                           aTGACCTCTTCATCGG‑CGCGCCTTAAGCCCGCCGA‑GAAGAGCCAGAGACCCCGAGCAAGAc                                                                                                             <  6:281957/61‑1 (MQ=255)
                                                                                                                           aTGACCTCTTCATCGG‑CGCGCCTTAAGCCCGCCGA‑GAAGAGCCAGAGACCCCGAGCAAGAc                                                                                                             >  5:281957/1‑61 (MQ=255)
                                                                                                                           aTGACCTCTTCATCGG‑CGCGCCTTAAGCCCGCCGA‑GAAGAGCCAGAGACCCCGAGCAAGACCCCTAACCGCCTTATGGCGGTTTCTCTGCCTCCCAGTCTGCTGCGAAGGCCGATAGCCGAGTCCAACCAATTCTATTTTTCGACCTgg                     >  7:21549/1‑149 (MQ=255)
                                                                                                                           aTGACCTCTTCATCGG‑CGCGCCTTAAGCCCGCCGA‑GAAGAGCCAGACACCCCGAGCAAGACCCCTAACCGCCTTATGGCCGTTTCTCTGCCTCCCCCTCTGCTGCGAAGGCCGAGAGCCGACTCCAACCAAATCTAATTTTCGACCTgg                     >  7:72814/1‑149 (MQ=255)
                                                                                                                                ctctTCATCGG‑CGCGCCTTAAGCCCGCCGA‑GAAGAGCCAGAGACCCCGAGCAAGACCCCTAACCTCCTTATGGCGGTTTCTCTGCCTCCCAGTCTGCTGCGAAGGCCGATATCCGAGTCCAACCAATTCTATTTTTCGACCTGGCCTGa                <  6:371603/149‑1 (MQ=255)
                                                                                                                                ctctTCATCGG‑CGCGCCTTAAGCCCGCCGA‑GAAGAGCCAGAGACCCCGAGCAAGACCCCTAACCGCCTTATGGCGGTTTCTCTGCCTCCCAGTCTGCTGCGAAGGCCGATAGCCGAGTCCAACCAATTCTATTTTTCGACCTGGCCt                  <  4:268968/147‑1 (MQ=255)
                                                                                                                                ctctTCATCGG‑CGCGCCTTAAGCCCGCCGA‑GAAGAGCCAGAGACCCCGAGCAAGACCCCTAACCGCCTTATGGCGGTTTCTCTGCCTCCCAGTCTGCTGCGAAGGCCGATAGCCGAGTCCAACCAATTCTATTTTTCGACCTGGCCt                  >  3:268968/1‑147 (MQ=255)
                                                                                                                                 tctTCATCGG‑CGCGCCTTAAGCCCGCCGA‑GAAGAGCCAGAGACCCCGAGCAAGACCACTAACAGCCTTATGGCGGTTTCTCTGCCTCCCAGTCTGCTGCGAAGGCCGATAGCCGAGTCCAACCAATTATATTTATCGACCAGGCCTGa                >  4:26935/1‑148 (MQ=255)
                                                                                                                                   ttgaTCGG‑CTCGCATTAAGCCCGCCGA‑GAATAGCCAGAGACCGCGAGCAAGACCCCTAACCGCCTTATGGCGGTTTCTCTGCCTCCCATTCTGCTGCGTATGCCGATAGCCGAGTCCAACCAATTCTATTTTTCGACCTGGCCTGAc               <  2:184448/144‑1 (MQ=255)
                                                                                                                                       tCGG‑CGGGCTTAAGGCGCGCCGATGAAGAGCCAGAGACCCCGAGCAAGACCCCTAACCGCCTTAt                                                                                              >  7:340932/1‑65 (MQ=18)
                                                                                                                                       tCGG‑CGGGCTTAAGGCGCGCCGATGAAGAGCCAGAGACCCCGAGCAAGACCCCTAACCGCCTTAt                                                                                              <  8:340932/65‑1 (MQ=18)
                                                                                                                                       tCGG‑CGCGCCTTAAGCCCGCCGA‑GAAGAGCCAGAGACCCCGAGCAAGACCCCTAACCGCCTTATGGCGGTTTCTCTGCCTCCCAGTCTGCTGCGAAGGCCGATAGCCGAGTCAAACCAATTCTATTTTTCGACCTGGCCTGACCGCAg          >  4:290801/1‑148 (MQ=255)
                                                                                                                                        cgg‑cggGCTTAAGGCGCGCCGA‑GAAGAGCCAGAGACCCCGAGCAAGACCCCTAACCGCCTTATGGCGGTTTCTCTGCCTCCCAGTCTGCTGCGAAGGCCGATAGCCGAGTCCAACCAATTCTATTTTTCGACCTGGCCTGACCGCAg          >  8:30875/1‑147 (MQ=255)
                                                                                                                                         gg‑CGCGCCTTAAGCCCGCCGA‑GAAGAGCCAGAGACCCCGAGCAAGACCCCTAACCGCCTTATGGCGGTTTCTCTGCCTCCCAGTCTGCTGCGAAGGCCGATAGCCGAGTCCAACCAATTCTATTTTTCGACCTGGCCTGACCGCAGTa        <  2:135736/148‑1 (MQ=255)
                                                                                                                                         gg‑CGCGCCTTAAGCCCGCCGA‑GAAGAGCCAGAGACCCCGAGCAAGACCCCTAACCGCCTTATGGCGGTTTCTCTGCCTCCCAGTCTGCTGCGAAGGCCGATAGCCGAGTCCAACCAATTCTATTTTTCGACCTGGCCTGACCGCAGTa        <  7:281611/148‑1 (MQ=255)
                                                                                                                                          g‑cgtgcCTTAAGCCCTCAGA‑GAAGATCCTGAGACCCCTAGCAAGACCCCTAACCGCCTTATGGCTGTTTCTCTGCCTCCCAGTCTGCTGCGAAGGCCGATAGCCGAGTCCAACCAATTGTATTTTTCGACCTGGCCTGACCGCAGTaga      <  7:344506/149‑1 (MQ=255)
                                                                                                                                             gcgcCTTAAGCCCGCCGA‑GAAGAGCCAGAGACCCCGAGCAAGACCCCTAACCGCCTTATGGCGGTTTCTCTGCCTCCCAGTCTGCTGCGAAGGCCGATAGCCGAGTCCAACCAATTCTATTTTTCGACCTGGCCTGACCGCAGTAGAGc    >  7:22694/1‑149 (MQ=255)
                                                                                                                                               gcCTTAAGCCCGCCGA‑GAAGAGCCAGAGACCCCGAGCAAGACCCCTAACCGCCTTATGGCGGTTTCTCTGCCTCCCAGTCTGCTGCGAAGGCCGATAGCCGAGTCCAACCAATTCTATTTTTCGACCTGGCCTGACCGCAGTAGAGCgt  <  8:52639/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                 
GTACTCCACACCCAGTCTCACATCTGACCCTGTCAGCGACGCTAATGACGGTGTTGATCCACAGAGGGTTAGGGATAGCCTTAAGGCGTAATTGTCTGATACCAACACCTCTGCGATGAGGACATGACCTCTTCATCGG‑CGCGCCTTAAGCCCGCCGA‑GAAGAGCCAGAGACCCCGAGCAAGACCCCTAACCGCCTTATGGCGGTTTCTCTGCCTCCCAGTCTGCTGCGAAGGCCGATAGCCGAGTCCAACCAATTCTATTTTTCGACCTGGCCTGACCGCAGTAGAGCGT  >  NC_002947/5041054‑5041344

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑