Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,041,202 A→G 7.8% intergenic (+180/‑482) PP_5680 → / → PP_4447 Cro/CI family transcriptional regulator/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,041,2020AG 7.8% 253.6 / 9.7 89intergenic (+180/‑482)PP_5680/PP_4447Cro/CI family transcriptional regulator/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (40/42);  new base G (3/4);  total (43/46)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.83e-01

CACACCCAGTCTCACATCTGACCCTGTCAGCGACGCTAATGACGGTGTTGATCCACAGAGGGTTAGGGATAGCCTTAAGGCGTAATTGTCTGATACCAACACCTCTGCGATGAGGACATGACCTCTTCATCGG‑CGCGCCTTAAGCCCGCCGA‑GAAGAGCCAGAGACCCCGAGCAAGACCCCTAACCGCCTTATGGCGGTTTCTCTGCCTCCCAGTCTGCTGCGAAGGCCGATAGCCGAGTCCAACCAATTCTATTTTTCGACCTGGCCTGACCGCAGTAGAGCGTGTGC  >  NC_002947/5041060‑5041348
                                                                                                                                               |                                                                                                                                                   
cacaCCCAGTCTCACATCTGACCCTGTCAGCGACGCTAATGACGGTGTTGATCCACAGAGGGTTAGGGATAGCCTTAAGGCGTAATTGTCTGATACCAACACCTCTGCGATGAGGACATGACCTCTTCATCGG‑CGCGCCTTAAGCccgc                                                                                                                                               >  6:89605/1‑149 (MQ=255)
    cccAGTCTCACATCTGACCCTGTCAGCGACGCTAATGACGGTGTTGATCCACAGAGGGTTAGGGATAGCCTTAAGGCGTAATTGTCTGATACCAACACCTCTGCGATGAGGACATGACCTCTTCATCGG‑CGCGCCTTAAGCccgcc                                                                                                                                              >  5:70333/1‑146 (MQ=255)
    cccAGTCTCACATCTGACCCTGTCAGCGACGCTAATGACGGTGTTGATCCACAGAGGGTTAGGGATAGCCTTAAGGCGTAATTGTCTGATACCAACACCTCTGCGATGAGGACATGACCTCTTCATCGG‑CGCGCCTTAAGCccgcc                                                                                                                                              <  6:70333/146‑1 (MQ=255)
       aGTCTCACATCTGACCCTGTCAGCGACGCTAATGACGGTGTTGATCCACAGAGGGTTAGGGATAGCCTTAAGGCGTAATTGTCTGATACCAACACCTCTGCGATGAGGACATGACCTCTTCATCGG‑CGGGCTTAAGGCGCGCCgatgaa                                                                                                                                        <  8:183197/149‑5 (MQ=255)
        gTCTCACATCTGACCCTGTCAGCGACGCTAATGACGGTGTTGATCCACAGAGGGTTAGGGATAGCCTTAAGGCGTAATTGTCTGATACCAACACCTCTGCGATGAGGACATGACCTCTTCATCGG‑CGCGCCTTAAGCCCGCCGa‑gaaga                                                                                                                                      <  2:276225/149‑1 (MQ=255)
        gTCTCACATCTGACCCTGTCAGCGACGCTAATGACGGTGTTGATCCACAGAGGGTTAGGGATAGCCTTAAGGCGTAATTGTCTGATACCAACACCTCTGCGATGAGGACATGACCTCTTCATCGG‑CGCGCCTTAAGCCCGCCGa‑gaaga                                                                                                                                      <  8:143623/149‑1 (MQ=255)
        gTCTCACATCTGACCCTGTCAGCGACGCTAATGACGGTGTTGATCCACAGAGGGTTAGGGATAGCCTTAAGGCGTAATTGTCTGATACCAACACCTCTGCGATGAGGACATGACCTCTTC‑TCGG‑CGGGCTTAAGGCGCGCCGATgaaga                                                                                                                                      <  3:149848/149‑1 (MQ=38)
         tctcACATCTGACCCTGTCAGCGACGCTAATGACGGTGTTGATCCACAGAGGGTTAGGGATAGCCTTAAGGCGTAATTGTCTGATACCAACACCTCTGCGATGAGGACATGACCTCTTCATCGG‑CGCGCCTTAAGCCCGCCGA‑GAagag                                                                                                                                     >  8:197380/1‑149 (MQ=255)
           tcACATCTGACCCTGTCAGCGACGGTAATGACGGTGTTGATCCACAGAGGGTTAGGGATAGCCTTAAGGCGTAATTGTCTGATACCAACACCTCTGCGATGAGGACATGACCTCTTCATCGG‑CGCGCCTTAAGCCCGCCGA‑GAAGAGc                                                                                                                                    >  7:52526/1‑148 (MQ=255)
           tcACATCTGACCCTGTCAGCGACGCTAATGACGGTGTTGATCCACAGAGGGTTAGGGATAGCCTTAAGGCGTAATTGTCTGATACCAACACCTCTGCGATGAGGACATGACCTCTTCATCGG‑CGCGCCTTAAGCCCGCCGA‑GAataga                                                                                                                                    >  8:146935/1‑147 (MQ=255)
            cacaTCTGACCCTGTCAGCGACGCTAATGACGGTGTTGATCCACAGAGGGTTAGGGATAGCCTTAAGGCGTAATTGTCTGATACCAACACCTCTGCGATGAGGACATGACCTCTTCATCGG‑CGCGCCTTAAGCCCGCCGA‑GAAGAGc                                                                                                                                    >  5:241560/1‑147 (MQ=255)
             acaTCTGACCCTGTCAGCGACGCTAATGACGGTGTTGATCCACAGAGGGTTAGGGATAGCCTTAAGGCGTAATTGTCTGATACCAACACCTCTGCGATGAGGACATGACCTCTTCATCGGCCGCGCCTTAAGCCCGCCGA‑GAAGAGCCa                                                                                                                                  <  1:205182/149‑1 (MQ=255)
             acaTCTGACCCTGTCAGCGACGCTAATGACGGTGTTGATCCACAGAGGGTTAGGGATAGCCTTAAGGCGTAATTGTCTGATACCAACACCTCTGCGATGAGGACATGACCTCTTCATCGG‑CGCGCCTTAAGCCCGCCGA‑GAAGAGc                                                                                                                                    >  6:139066/1‑146 (MQ=255)
             acaTCTGACCCTGTCAGCGACGCTAATGACGGTGTTGATCCACAGAGGGTTAGGGATAGCCTTAAGGCGTAATTGTCTGATACCAACACCTCTGCGATGAGGACATGACCTCTTCATCGG‑CGCGCCTTAAGCCCGCCGA‑GAAGAGCCa                                                                                                                                  <  8:52526/148‑1 (MQ=255)
             aaaTCTGACCCTGTCAGCGACCCTAATGACGGTGTTGATCCAGAGAGGGTTAGGGAAATCCTTAAGGCTTAATTGTCTGATACCAACTCCTCTGCGATGATTACATGACATCTTCATCGT‑CGGGCCTTAAGCCCTCAGA‑GAATAGCCag                                                                                                                                 <  8:341501/147‑1 (MQ=255)
              caTCTGACCCTGTCAGCGACGCTAATGACGGTGTTGATCCACAGAGGGTTAGGGATAGCCTTAAGGCGTAATTGTCTGATACCAACACCTCTGCGATGAGGACATGACCTCTTC‑TCGG‑CGGGCTTAAGGCGCGCCgatgaa                                                                                                                                        <  1:334048/141‑5 (MQ=255)
              caTCTGACCCTGTCAGCGACGCTAATGACGGTGTTGATCCACAGAGGGTTAGGGATAGCCTTAAGGCGTAATTGTCTGATACCAACACCTCTGCGATGAGGACATGACCTCTTC‑TCGG‑CGGGCTTAAGGCGCGCCgatgaa                                                                                                                                        >  2:334048/1‑137 (MQ=255)
               aTCTGACCCTGTCAGCGACGCTAATGACGGTGTTGATCCACAGAGGGTTAGGGATAGCCTTAAGGCGTAATTGTCTGATACCAACACCTCTGCGATGAGGACATGACCTCTTCATCGG‑CGCGCCTTAAGCCCGCCGA‑GAAGAGCCagag                                                                                                                               >  8:276134/1‑149 (MQ=255)
                    aCCCTGTCAGCGACGCTAATGACGGTGTTGATCCACAGAGGGTTAGGGATAGCCTTAAGGCGTAATTGTCTGATACCAACACCTCTGCGATGAGGACATGACCTCTTCATCGG‑CGCGCCTTAAGCCCGCCGA‑GAAGAGCCAGAGAcccc                                                                                                                          >  1:305695/1‑149 (MQ=255)
                      ccTGTCAGCGACGCTAATGACGGTGTTGATCCACAGAGGGTTAGGGATAGCCTTAAGGCGTAATTGTCTGATACCAACACCTCTGCGATGAGGACATGACCTCTTCTTCGG‑CGCGCCTTAAGCCCGCCGA‑GAAGAGCCAGAGACCCCGa                                                                                                                        <  1:256903/149‑1 (MQ=255)
                      ccTGTCAGCGACGCTAATGACGGTGTTGATCCACAGAGGGTTAGGGATAGCCTTAAGGCGTAATTGTCTGATACCAACACCTCTGCGATGAGGACATGACCTCTTCATCGG‑CGCGCCTTaa                                                                                                                                                     <  1:294694/121‑1 (MQ=255)
                      ccTGTCAGCGACGCTAATGACGGTGTTGATCCACAGAGGGTTAGGGATAGCCTTAAGGCGTAATTGTCTGATACCAACACCTCTGCGATGAGGACATGACCTCTTCATCGG‑CGCGCCTTaa                                                                                                                                                     >  2:294694/1‑121 (MQ=255)
                      ccTGTCAGCGACGCTAATGACGGTGTTGATCCACAGAGGGTTAGGGATAGCCTTAAGGCGTAATTGTCTGATACCAACACCTCTGCGATGAGGACATGACCTCTTCATCGG‑CGCGCCTTAAGCCCGCCGA‑GAAGAGCCAGAGACCCCGa                                                                                                                        >  2:116590/1‑149 (MQ=255)
                      ccTGTCAGCGACGCTAATGACGGTATTGATCCACAGAGGGTTTGGGAAAGCCTTAAGGCGTAATTTTCTGATACCAACACCTCTGGGATGAGGACATGACCTCTTCAACCG‑CGCGCCTTAAGCCCGCCGA‑GAAAAGCCAGAGCcccccc                                                                                                                        >  7:52639/1‑147 (MQ=255)
                       cTGTCAGCGACGCTAATGACGGTGTTGATCCACAGAGGGTTAGGGATAGCCTTAAGGCGTAATTGTCTGATACCAACACCTCTGCGATGAGGACATGACCTCTTCATCGG‑CGCGCCTTAAGCCCGCCGA‑GAAGAGCCAGAGACCCCGAg                                                                                                                       <  1:33976/149‑1 (MQ=255)
                           cAGCGACGCTAATGACGGTGTTGATCCACAGAGGGTTAGGGATAGCCTTAAGGCGTAATTGTCTGATACCAACACCTCTGCGATGAGGACATGACCTCTTCATCGG‑CGCGCCTTAAGACAGCCGA‑GAAGAGCCAGAGACCCCGAGc                                                                                                                      >  8:307353/1‑146 (MQ=255)
                            aGCGACGCTAATGACGGTGTTGATCCACAGAGGGTTAGGGATAGCCTTAAGGCGTAATTGTCTGATACCAACACCTCTGCGATGAGGACATGACCTCTTCATCGG‑CGCGCCTTAAGCCCGCCGA‑GAAGAGCCAGAGACCCCGAGCAAGa                                                                                                                  <  2:305695/149‑1 (MQ=255)
                             gCGACGCTAATGACGGTGTTGATCCACAGAGGGTTAGGGATAGCCTTAAGGCGTAATTGTCTGATACCAACACCTCTGCGATGAGGACATGACCTCTTCATCGG‑CGCGCCTTAAGCCCGCCGA‑GAAGAGCCAGAGACCCCGAGCAAGAc                                                                                                                 <  5:89605/149‑1 (MQ=255)
                             gCGACGCTAATGACGGTGTTGATCCACAGAGGGTTAGGGATAGCCTTAAGGCGTAATTGTCTGATACCAACACCTCTGCGATGAGGACATGACCTCTTCATCGG‑CGCGCCTTAAGCCCGCCGA‑GAAGAGCCAGAGACCCCGAGCAAGAc                                                                                                                 >  1:135736/1‑149 (MQ=255)
                               gACGCTAATGACGGTGTTGATCCACAGAGGGTTAGGGATAGCCTTAAGGCGTAATTGTCTGATACCAACACCTCTGCGATGAGGACATGACCTCTTCATCGG‑CGCGCCTTAAGCCCGCCGA‑GAAGAGCCAGAGACCCCGAGCAAGAccc                                                                                                               <  6:155051/149‑1 (MQ=255)
                                aCGCTAATGACGGTGTTGATCCACAGAGGGTTAGGGATAGCCTTAAGGCGTAATTGTCTGATACCAACACCTCTGCGATGAGGACATGACCTCTTCATCGG‑CGCGCCTTAAGCCCGCCGA‑GAAGAGCCAGAGACCCCGAGCAAGAcccc                                                                                                              <  3:170863/149‑1 (MQ=255)
                                    tAATGACGGTGTTGATCCACAGAGGGTTAGGGATAGCCTTAAGGCGTAATTGTCTGATACCAACACCTCTGCGATGAGGACATGACCTCTTCATCGG‑CGCGCCTTAAGCCCGCCGA‑GAAGAGCCAGAGACCCCGAGCAAGACCCCTAAc                                                                                                          >  3:362680/1‑149 (MQ=255)
                                    tAATGACGGTGTTGATCCACAGAGGGTTAGGGATAGCCTTAAGGCGTAATTGTCTGATACCAACACCTCTGCGATGAGGACATGACCTCTTCATCGG‑CGCGCCTTAAGCCCGCCGA‑GAAGAGCCAGAGACCCCGAGCAAGACCCCTAAc                                                                                                          >  2:307907/1‑149 (MQ=255)
                                    tAATGACGGTGTTGATCCACAGAGGGTTAGGGATAGCCTTAAGGCGTAATTGTCTGATACCAACACCTCTGCGATGAGGACATGACCTCTTAATCGG‑CGCGCCTTAAGCCCGCCGA‑GAAGAGCCAGAGACCCCGAGCAACACACCTAAc                                                                                                          >  1:184448/1‑149 (MQ=255)
                                        gACGGTGTTGATCCACAGAGGGTTAGGGATAGCCTTAAGGCGTAATTGTCTGATACCAACACCTCTGCGATGAGGACATGACCTCTTCATCGG‑CGCGCCTTAAGCCCGCCGA‑GAAGAGCCAGAGACCCCGAGCAAGACCCCTAACCGcc                                                                                                      >  6:274469/1‑149 (MQ=255)
                                         aCGGTGTTGATCCACAGAGGGTTAGGGATAGCCTTAAGGCGTAATTGTCTGATACCAACACCTCTGCGATGAGGACATGACCTCTTCATCGG‑CGCGCCTTAAGCCCGCCGA‑GAAGAGCCAGAGACCCCGAGCAAGACCCCTAACCGCCt                                                                                                     >  4:198455/1‑149 (MQ=255)
                                         aCGGTGTTGATCCACAGAGGGTTAGGGATAGCCTTAAGGCGTAATTGTCTGATACCAACACCTCTGCGATGAGGACATGACCTCTTCATCGG‑CGCGCCTTAAGCCCGCCGA‑GAAGAGCCAGAGACCCCGAGCAAGACCCCTAACCGCCt                                                                                                     >  6:273753/1‑149 (MQ=255)
                                           ggTGTTGATCCACAGAGGGTTAGGGATAGCCTTAAGGCGTAATTGTCTGATACCCACACCTCTGCGATGAGGACATGACCTCTTCATCGG‑CGCGCCTTAAGCCCGCCGA‑GAAGAGCCAGAGACCCCGAGCAAGACCCCTAACCGCCTTa                                                                                                   >  8:281611/1‑149 (MQ=255)
                                                   tCCACAGAGGGTTAGGGATAGCCTTAAGGCGTAATTGTCTGATACCAACACCTCTGCGATGAGGACATGACCTCTTCATCGG‑CGCGCCTTAAGCCCGCCGA‑GAAGAGCCAGAGACCCCGAGCAAGACCCCTAACCGCCATAAGGCGGt                                                                                            <  3:66048/148‑1 (MQ=255)
                                                     cacaGAGGGTTAGGGATAGCCTTAAGGCGTAATTGTCTGATACCAACACCTCTGCGATGAGGACATGACCTCTTCATCGG‑CGCGCCTTAAGCCCGCCGA‑GAAGAGCCAGAGACCCCGAGCAAGACCCCTAACCGCCTTATGGCGGTTtc                                                                                         >  7:6954/1‑149 (MQ=255)
                                                      acaGAGGGTTAGGGATAGCCTTAAGGCGTAATTGTCTGATACCAACACCTCTGCGATGAGGACATGACCTCTTCATCGG‑CGCGCCTTAAGCCCGCCGA‑GAAGAGCCAGAGACCCCGAGCAAGACCCCTAACCGCCTTATGGCGGTTtct                                                                                        <  5:8284/149‑1 (MQ=255)
                                                       caGAGGGTTAGGGATAGCCTTAAGGCGTAATTGTCTGATACCAACACCTCTGCGATGAGGACATGACCTCTTCATCGG‑CGCGCCTTAAGCCCGCCGA‑GAAGAGCCAGAGACCCCGAGCAAGACCCCTAACCGCCTTATGGCGGTTtctc                                                                                       <  3:125378/149‑1 (MQ=255)
                                                        agagGGTTAGGGATAGCCTTAAGGCGTAATTGTCTGATACCAACACCTCTGCGATGAGGACATGACCTCTTCATCGG‑CGCGCCTTAAGCCCGCCGA‑GAAGAGCCAGAGACCCCGAGCAAGACCCCTAACCGCCTTATGGCGGTTTctct                                                                                      <  7:276134/149‑1 (MQ=255)
                                                            ggTTAGGGATAGCCTTAAGGCGTAATTGTCTGATACCAACACCTCTGCGATGAGGACATGACCTCTTCATCGG‑CGCGCCTTAAGCCCGCCGA‑GAAGAGCCAGAGACCCCGAGCAAGACCCCTAACCGCCTTATGGCGGTTTCTCTGCCt                                                                                  <  7:146935/149‑1 (MQ=255)
                                                              ttAGGGATAGCCTTAAGGCGTAATTGTCTGATACCAACACCTCTGCGATGAGGACATGACCTCTTCATCGG‑CGCGCCTTAAGCCCGCCGA‑GAAGAGCCAGAGACCCCGAGCAAGACCCCTAACCGCCTTATGGCGGTTTCTCTGCCTcc                                                                                <  1:116590/149‑1 (MQ=255)
                                                                 gggATAGCCTTAAGGCGTAATTGTCTGATACCAACACCTCTGCGATGAGGACATGACCTCTTCATCGG‑CGCGCCTTAAGCCCGCCGA‑GAAGAGCCAGAGACCCCGAGCAAGACCCCTAACCGCCTTATGGCGGTTTCTCTGCCTCCCa                                                                              <  5:274469/148‑1 (MQ=255)
                                                                  ggATAGCCTTAAGGCGTAATTGTCTGATACCAACACCTCTGCGATGAGGACATGACCTCTTCATCGG‑CGCGCCTTAAGCCCGCCGA‑GAAGAGCCAGAGACCCCGAGCAAGACCCCTAACCGCCTTATGGCGGTTTCTCTGCCTCCCAGt                                                                            >  4:212281/1‑149 (MQ=255)
                                                                    aTAGCCTTAAGGCGTAATTGTCTGATACCAACACCTCTGCGATGAGGACATGACCTCTTCATCGG‑CGCGCCTTAAGCCCGCCGA‑GAAGAGCCAGAGACCCCGAGCAAGAc                                                                                                                 >  5:152076/1‑110 (MQ=255)
                                                                    aTAGCCTTAAGGCGTAATTGTCTGATACCAACACCTCTGCGATGAGGACATGACCTCTTCATCGG‑CGCGCCTTAAGCCCGCCGA‑GAAGAGCCAGAGACCCCGAGCAAGAc                                                                                                                 <  6:152076/110‑1 (MQ=255)
                                                                    aTAGCCTTAAGGCGTAATTGTCTGATACCAACACCTCTGCGATGAGGACATGACCTCTTCATCGG‑CGCGCCTTAAGCCCGCCGA‑GAAGAGCCAGAGACCCCGAGCAAGACCCCTAACCGCCTTATGGCGGTTTCTCTGCCTCCCAGTct                                                                          >  2:217221/1‑149 (MQ=255)
                                                                     tAGCCTTAAGGCGTAATTGTCTGATACCAACACCTCTGCGATGAGGACATGACCTCTTCATCGG‑CGCGCCTTAAGCCCGCCGA‑GAAGAGCCAGAGACCCCGAGCAAGACCCCTAACCGCCTTATGGCGGTTTctct                                                                                      >  3:289286/1‑136 (MQ=255)
                                                                     tAGCCTTAAGGCGTAATTGTCTGATACCAACACCTCTGCGATGAGGACATGACCTCTTCATCGG‑CGCGCCTTAAGCCCGCCGA‑GAAGAGCCAGAGACCCCGAGCAAGACCCCTAACCGCCTTATGGCGGTTTctct                                                                                      <  4:289286/136‑1 (MQ=255)
                                                                       gCCTTAAGGCGTAATTGTCTGATACCAACACCTCTGCGATGAGGACATGACCTCTTCATCGG‑CGCGCCTTAAGCCCGCCGA‑GAAGAGCCAGAGACCCCGAGCAAGACCCCTAACCGCCTTATGGCGGTTTCTCTGCCTCCCAGTctgct                                                                       <  3:212281/149‑1 (MQ=255)
                                                                          ttAAGGCGTAATTGTCTGATACCAACACCTCTGCGATGAGGACATGACCTCTTCATCGG‑CGCGCCTTAAGCCCGCCGA‑GAAGAGCCAGAGACCCCGAGCAAGACCCCTAACCGCCTTATGGCGGTTTCTCTGCCTCCCAGTCtgctgc                                                                     >  5:147732/1‑148 (MQ=255)
                                                                             aGGCGTAATTGTCTGATACCAACACCTCTGCGATGAGGACATGACCTCTTCATCGG‑CGCGCCTTAAGCCCGCCGA‑GAAGAGCCAGAGACCCCGAGCAAGACCCCTAACCGCCTTATGGCGGTTTCTCTGCCTCCCAGTCTGCTGCGaa                                                                  <  1:217221/148‑1 (MQ=255)
                                                                                 gTAATTGTCTGATACCAACACCTCTGCGATGAGGACATGACCTCTTCATCGG‑CGCGCCTTAAGCCCGCCGA‑GAAGAGCCAGAGACCCCGAGCAAGACCCCTAACCGCCTTATGGCGGTTTCTCTGCCTCCCAGTCTGCTGCGAAGGCCg                                                             <  6:278440/149‑1 (MQ=255)
                                                                                  tAATTGTCTGATACCAACACCTCTGCGATGAGGACATGACCTCTTCATCGG‑CGCGCCTTAAGCCCGCCGA‑GAAGAGCCAGAGACCCCGAGCAAGACCCCTAACCGCCTTATGGCGGTTTCTCTGCCTCCCAGTCTGCTGCGAAGGCCGa                                                            <  4:362680/149‑1 (MQ=255)
                                                                                    aTTGTCTGATACCAACACCTCTGCGATGAGGACATGACCTCTTCATCGG‑CGCGCCTTAAGCCCGCCGA‑GAAGAGCCAGAGACCCCGAGCAAGACCCCTAACCGCCTTATGGCGGTTTCTCTGCCTCCCAGTCTGCTGCGAAGGCCGa                                                            >  1:177550/1‑147 (MQ=255)
                                                                                    aTTGTCTGATACCAACACCTCTGCGATGAGGACATGACCTCTTCATCGG‑CGCGCCTTAAGCCCGCCGA‑GAAGAGCCAGAGACCCCGAGCAAGACCCCTAACCGCCTTATGGCGGTTTCTCTGCCTCCCAGTCTGCTGCGAAGGCCGATa                                                          <  8:6954/149‑1 (MQ=255)
                                                                                       gTCTGATACCAACACCTCTGCGATGAGGACATGACCTCTTCATCGG‑CGCGCCTTAAGCCCGCCGA‑GAAGAGCCAGAGACCCCGAGCAAGACCCCTAACCGCCTTATGGCGGTTTCTCTGCCTCCCAGTCTGCTGCGAAGGCCGATAGc                                                        <  5:273753/148‑1 (MQ=255)
                                                                                       gTCTGATACCAACACCTCTGCGATGAGGACATGACCTCTTCATCGG‑CGCGCCTTAAGCCCGCCGA‑GAAGAGCCAGAGACCCCGAGCAAGACCCCTAACCGCCTTATGGCGGTTTCTCTGCCTCCCAGTCTGCTGCGAAGGCCGATAGc                                                        <  7:197380/148‑1 (MQ=255)
                                                                                        tCTGATACCAACACCTCTGCGATGAGGACATGACCTCTTCATCGG‑CGCGCCTTAAGCCCGCCGA‑GAAGAGCCAGAGACCCCGAGCAAGACCCCTAACCGCCTTATGGCGGTTTCTCTGCCTCCCAGTCTGCTGCGAAGGCCGATAGcc                                                       <  1:307907/148‑1 (MQ=255)
                                                                                           gATACCAACACCTCTGCGATGAGGACATGACCTCTTCATCGG‑CGCGCCTTAAGCCCGCCGA‑GAAGAGCCAGAGACCCCGAGCAAGACCCCTAACCGCCTTATg                                                                                                 <  3:357799/103‑1 (MQ=255)
                                                                                           gATACCAACACCTCTGCGATGAGGACATGACCTCTTCATCGG‑CGCGCCTTAAGCCCGCCGA‑GAAGAGCCAGAGACCCCGAGCAAGACCCCTAACCGCCTTATg                                                                                                 >  4:357799/1‑103 (MQ=255)
                                                                                            aTACCAACACCTCTGCGATGAGGACATGACCTCTTCATCGG‑CGCGCCTTAAGCCCGCCGA‑GAAGAGCCAGAGACCCCGAGCAAGACCCCTAACCGCCTTATGGCGGTTTCTCTGCCTCCCAGTCTGCTGCGAAGGCCGATAGCCGa                                                     <  3:198455/146‑1 (MQ=255)
                                                                                            aTACCAACACCTCTGCGATGAGGACATGACCTCTTCATCGG‑CGCGCCTTAAGCCCGCCGA‑GAAGAGCCAGAGACCCCGAGCAAGACCCCTAACCGCCTTATGGCGGTTTCTCTGCCTCCCAGTCTGCTGCGAAGGCCGATAGCCGa                                                     <  6:147732/146‑1 (MQ=255)
                                                                                                cAACACCTCTGCGATGAGGACATGACCTCTTCATCGG‑CGCGCCTTAAGCCCGCCGA‑GAAGAGCCAGAGACCCCGAGCAAGACCCCTAACCGCCTTATGGCGGTTTCTCTGCCTCCCAGTCTGCTGCGAAGGCCGATAGCCGAGTccaac                                              >  3:308726/1‑149 (MQ=255)
                                                                                                cAACACCTCTGCGATGAGGACATGACCTCTTCATCGG‑CGCGCCTTAAGCCCGCCGA‑GAAGAGCCAGAGACCCCGAGCAAGACCCCTAACCGCCTTATGGCGGTTTCTCTGCCTCCCAGTCTGCTGCGAAGGCCGATAGCCGAGTccaac                                              <  6:241560/149‑1 (MQ=255)
                                                                                                                 ggACATGACCTCTTCATCGG‑CGCGCCTTAAGCCCGCCGA‑GAAGAGCCAGAGACCCCGAGCAAGACCCCTAACCGCCTTATGGCGGTTTCTCTGCCTCCCAGTCTGCTGCGAAGGCCGATAGCCGAGTCCAACCAATTCTATTTTTCGAc                             >  7:303126/1‑149 (MQ=255)
                                                                                                                 ggACATGACCTCTTCATCGG‑CGCGCCTTAAGCCCGCCGA‑GAAGAGACAGAGACCCCGAGCAAGACCCCTAACCGCCTTATGGCGGTTTCTCTGCCTCCCAGTCTGCTGCGAAGGCCGATAGCCGAGTCCAACCAATTCTATTTTTCGAc                             >  4:260391/1‑149 (MQ=255)
                                                                                                                  gACATGACCTCTTCATCGG‑CGCGCCTTAAGCCCGCCGA‑GAAGAGCCAGAGACCCCGAGCAAGACCCCTAACCGCCTTATGGCGGTTTCTCTGCCTCCCAGTCTGCTGCGAAGGCCGATAGCCGAGTCCAACCAATTCTATTTTTCGAcc                            >  5:23592/1‑149 (MQ=255)
                                                                                                                  gACATGACCTCTTCATCGG‑CGCGACTTAAGCCAGCCGA‑GAACAGCGAGAGACACCGAGCAAGCCCCCTAACCGCCATATGGCGGTTTCTCTTCCTCCAAGTATGCTGGGACTGCAGATATCCGAGTCCCCCAAATGCTATTTTTAGAcc                            >  4:184369/1‑149 (MQ=255)
                                                                                                                    cATGACCTCTTCATCGG‑CGCGCCTTAAGCCCGCCGA‑GAAGAGCCAGAGACCCCGAGCAAGACCCCTAACCGCCTTATGGCGGTTTCTCTGCCTCCCAGTCTGCTGCGAAGGCCGATAGCCGAGTCCAACCAATTCTATTTTTCGACCTg                          <  6:23592/149‑1 (MQ=255)
                                                                                                                     aTGACCTCTTCATCGG‑CGCGCCTTAAGCCCGCCGA‑GAAGAGCCAGAGACCCCGAGCAAGAc                                                                                                                 <  6:281957/61‑1 (MQ=255)
                                                                                                                     aTGACCTCTTCATCGG‑CGCGCCTTAAGCCCGCCGA‑GAAGAGCCAGAGACCCCGAGCAAGAc                                                                                                                 >  5:281957/1‑61 (MQ=255)
                                                                                                                     aTGACCTCTTCATCGG‑CGCGCCTTAAGCCCGCCGA‑GAAGAGCCAGAGACCCCGAGCAAGACCCCTAACCGCCTTATGGCGGTTTCTCTGCCTCCCAGTCTGCTGCGAAGGCCGATAGCCGAGTCCAACCAATTCTATTTTTCGACCTgg                         >  7:21549/1‑149 (MQ=255)
                                                                                                                     aTGACCTCTTCATCGG‑CGCGCCTTAAGCCCGCCGA‑GAAGAGCCAGACACCCCGAGCAAGACCCCTAACCGCCTTATGGCCGTTTCTCTGCCTCCCCCTCTGCTGCGAAGGCCGAGAGCCGACTCCAACCAAATCTAATTTTCGACCTgg                         >  7:72814/1‑149 (MQ=255)
                                                                                                                          ctctTCATCGG‑CGCGCCTTAAGCCCGCCGA‑GAAGAGCCAGAGACCCCGAGCAAGACCCCTAACCTCCTTATGGCGGTTTCTCTGCCTCCCAGTCTGCTGCGAAGGCCGATATCCGAGTCCAACCAATTCTATTTTTCGACCTGGCCTGa                    <  6:371603/149‑1 (MQ=255)
                                                                                                                          ctctTCATCGG‑CGCGCCTTAAGCCCGCCGA‑GAAGAGCCAGAGACCCCGAGCAAGACCCCTAACCGCCTTATGGCGGTTTCTCTGCCTCCCAGTCTGCTGCGAAGGCCGATAGCCGAGTCCAACCAATTCTATTTTTCGACCTGGCCt                      >  3:268968/1‑147 (MQ=255)
                                                                                                                          ctctTCATCGG‑CGCGCCTTAAGCCCGCCGA‑GAAGAGCCAGAGACCCCGAGCAAGACCCCTAACCGCCTTATGGCGGTTTCTCTGCCTCCCAGTCTGCTGCGAAGGCCGATAGCCGAGTCCAACCAATTCTATTTTTCGACCTGGCCt                      <  4:268968/147‑1 (MQ=255)
                                                                                                                           tctTCATCGG‑CGCGCCTTAAGCCCGCCGA‑GAAGAGCCAGAGACCCCGAGCAAGACCACTAACAGCCTTATGGCGGTTTCTCTGCCTCCCAGTCTGCTGCGAAGGCCGATAGCCGAGTCCAACCAATTATATTTATCGACCAGGCCTGa                    >  4:26935/1‑148 (MQ=255)
                                                                                                                             ttgaTCGG‑CTCGCATTAAGCCCGCCGA‑GAATAGCCAGAGACCGCGAGCAAGACCCCTAACCGCCTTATGGCGGTTTCTCTGCCTCCCATTCTGCTGCGTATGCCGATAGCCGAGTCCAACCAATTCTATTTTTCGACCTGGCCTGAc                   <  2:184448/144‑1 (MQ=255)
                                                                                                                                 tCGG‑CGGGCTTAAGGCGCGCCGATGAAGAGCCAGAGACCCCGAGCAAGACCCCTAACCGCCTTAt                                                                                                  >  7:340932/1‑65 (MQ=18)
                                                                                                                                 tCGG‑CGGGCTTAAGGCGCGCCGATGAAGAGCCAGAGACCCCGAGCAAGACCCCTAACCGCCTTAt                                                                                                  <  8:340932/65‑1 (MQ=18)
                                                                                                                                 tCGG‑CGCGCCTTAAGCCCGCCGA‑GAAGAGCCAGAGACCCCGAGCAAGACCCCTAACCGCCTTATGGCGGTTTCTCTGCCTCCCAGTCTGCTGCGAAGGCCGATAGCCGAGTCAAACCAATTCTATTTTTCGACCTGGCCTGACCGCAg              >  4:290801/1‑148 (MQ=255)
                                                                                                                                  cgg‑cggGCTTAAGGCGCGCCGA‑GAAGAGCCAGAGACCCCGAGCAAGACCCCTAACCGCCTTATGGCGGTTTCTCTGCCTCCCAGTCTGCTGCGAAGGCCGATAGCCGAGTCCAACCAATTCTATTTTTCGACCTGGCCTGACCGCAg              >  8:30875/1‑147 (MQ=255)
                                                                                                                                   gg‑CGCGCCTTAAGCCCGCCGA‑GAAGAGCCAGAGACCCCGAGCAAGACCCCTAACCGCCTTATGGCGGTTTCTCTGCCTCCCAGTCTGCTGCGAAGGCCGATAGCCGAGTCCAACCAATTCTATTTTTCGACCTGGCCTGACCGCAGTa            <  2:135736/148‑1 (MQ=255)
                                                                                                                                   gg‑CGCGCCTTAAGCCCGCCGA‑GAAGAGCCAGAGACCCCGAGCAAGACCCCTAACCGCCTTATGGCGGTTTCTCTGCCTCCCAGTCTGCTGCGAAGGCCGATAGCCGAGTCCAACCAATTCTATTTTTCGACCTGGCCTGACCGCAGTa            <  7:281611/148‑1 (MQ=255)
                                                                                                                                    g‑cgtgcCTTAAGCCCTCAGA‑GAAGATCCTGAGACCCCTAGCAAGACCCCTAACCGCCTTATGGCTGTTTCTCTGCCTCCCAGTCTGCTGCGAAGGCCGATAGCCGAGTCCAACCAATTGTATTTTTCGACCTGGCCTGACCGCAGTaga          <  7:344506/149‑1 (MQ=255)
                                                                                                                                       gcgcCTTAAGCCCGCCGA‑GAAGAGCCAGAGACCCCGAGCAAGACCCCTAACCGCCTTATGGCGGTTTCTCTGCCTCCCAGTCTGCTGCGAAGGCCGATAGCCGAGTCCAACCAATTCTATTTTTCGACCTGGCCTGACCGCAGTAGAGc        >  7:22694/1‑149 (MQ=255)
                                                                                                                                         gcCTTAAGCCCGCCGA‑GAAGAGCCAGAGACCCCGAGCAAGACCCCTAACCGCCTTATGGCGGTTTCTCTGCCTCCCAGTCTGCTGCGAAGGCCGATAGCCGAGTCCAACCAATTCTATTTTTCGACCTGGCCTGACCGCAGTAGAGCgt      <  8:52639/149‑1 (MQ=255)
                                                                                                                                              aaGCCCGCCGA‑GAAGAGCCAGAGACCCCGAGCAAGACCCCTAACCGCCTTATGGCGGTTTCTCTGCCTCCCAGTCTGCTGCGAAGGCCGATAGCCGAGTCCAACCAATTCTATTTTTCGACCTGGCCTGACCGCAGTAGAGCGTGTGc  <  4:308726/148‑1 (MQ=255)
                                                                                                                                               |                                                                                                                                                   
CACACCCAGTCTCACATCTGACCCTGTCAGCGACGCTAATGACGGTGTTGATCCACAGAGGGTTAGGGATAGCCTTAAGGCGTAATTGTCTGATACCAACACCTCTGCGATGAGGACATGACCTCTTCATCGG‑CGCGCCTTAAGCCCGCCGA‑GAAGAGCCAGAGACCCCGAGCAAGACCCCTAACCGCCTTATGGCGGTTTCTCTGCCTCCCAGTCTGCTGCGAAGGCCGATAGCCGAGTCCAACCAATTCTATTTTTCGACCTGGCCTGACCGCAGTAGAGCGTGTGC  >  NC_002947/5041060‑5041348

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑