Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,050,009	A→T	5.5%	L135I (TTA→ATA) ‡	PP_5686 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,050,009	0	A	T	5.5%	259.9 / 2.5	84	L135I (TTA→ATA) ‡	PP_5686	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (39/40);  new base T (2/3);  total (41/43)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TACCCTTAAAAGCCTGCTTCATGTCTTTGCGATAGTTCTTCCTACTCAACCGCCTGGCGGAAATAAATCTAAATCTTCCACTCACCATCGTCCCAATTGACATCGTATAAATAACACCTGAAACTTTCTTAACTGGAAAGGATAAATTCTTTCCATTTTCACCAGCGTCGAGATCAGAAAACATCACAGTGCGGCGACCATCTCTCGGATGAGGACAAACGACTGATAATGTACCAAACTTTTCCGAGTAGCTGACTACTTCGTTAAAGCTTAATCCATGCTTGAT  >  NC_002947/5049865‑5050150                                                                                                                                                 |                                                                                                                                              taCCCTTAAAAGCCTGCTTCATGTCTTTGCGATAGTTCTTCCTACTCAACCGCCTGGCGGAAATAAATCTAAATCTTCCACTCACCATCGTCCCAATTGACATCGTATAAATAACACCTGAAACTTTCTTAACTGGAAAGGATAAATTc                                                                                                                                           >  4:30629/1‑149 (MQ=255)    ccTTAAAAGCCTGCTTCATGTCTTTGCGATAGTTCTTCCTACTCAACCGCCTGGCGGAAATAAATCTAAATCTTCCACTCACCATCGTCCCAATTGACATCGTATAAATAACACCTGAAACTTTCTTAACTGGAAAGGATAAATTCttt                                                                                                                                        >  8:212012/1‑149 (MQ=255)    ccTTAAAAGCCTGCTTCATGTCTTTGCGATAGTTCTTCCTACTCAACCGCCTGGCGGAAATAAATCTAAATCTTCCACTCACCATCGTCCCAATTGACATCGTATAAATAACACCTGAAACTTTCTTAACTGGAAAGGATAAATTCtt                                                                                                                                         >  1:166523/1‑148 (MQ=255)    ccTTAAAAGCCTGCTTCATGTCTTTGCGATAGTTCTTCCTACTCAACCGCCTGGCGGAAATAAATCTAAATCTTCCACTCACCATCGTCCCAATTGACATCGTATAAATAACACCTGAAACTTTCTTAACTGGAAAGGATAAATTCtt                                                                                                                                         >  7:321818/1‑148 (MQ=255)    ccTTAAAAGCCTGCTTCATGTCTTTGCGATAGTTCTTCATACTCAACAGCCTGGCGGAAATAAATCTAAATCTTCCACTCACCATCGTCCCAATTGACATAGTATAAATAAAACCTGAAACTTTATTAACTGGAAATGATAAattatt                                                                                                                                         >  5:210619/1‑148 (MQ=255)     cTTAAAAGCCTGCTTCATGTCTTTGCGATAGTTCTTCCTACTCAACCGCCTGGCGGAAATAAATCTAAATCTTCCACTCACCATCGTCCCAATTGACATCGTATAAATAACACCTGAAACTTTCTTAACTGGAAAGGATAAATTCTTTc                                                                                                                                       >  6:229292/1‑149 (MQ=255)      ttAAAAGCCTGCTTCATGTCTTTGCGATAGTTCTTCCTACTCAACCGCCTGGCGGAAATAAATCTAAATCTTCCACTCACCATCGTCCCAATTGACATCGTATAAATAACACCTGAAACTTTCTTAACTGGAAAGGATAAATTCTTTc                                                                                                                                       >  1:329618/1‑148 (MQ=255)         aaaGCCTGCTTCATGTCTTTGCGATAGTTCTTCCTACTCAACCGCCTAGCGGAAATAAATCTAAATCTTCCACTCACCATCGTCCCAATTGACATCGTATAAATAACACCTGAAACTTTCTTAACTGGAAAGGATAAATTCTTTCCAtt                                                                                                                                   >  1:268766/1‑149 (MQ=255)            gCCTGCTTCATGTCTTTGCGATAGTTCTTCCTACTCAACCGCCTGGCGGAAATAAATCTAAATCTTCCACTCACCATCGTCCCAATTGACATCGTATAAATAACACCTGAAACTTTCTTAACTGGAAAGGATAAATTCTTTCCATTTTc                                                                                                                                <  2:166523/149‑1 (MQ=255)                 cTTCATGTCTTTGCGATAGTTCTTCCTACTCAACCGCCTGGCGGAAATAAATCTAAATCTTCCACTCACCATCGTCCCAATTGACATCGTATAAATAACACCTGAAACTTTCTTAACTGGAAAGGATAAATTCTTTCCATTTTCACCa                                                                                                                            <  7:266070/148‑1 (MQ=255)                 cTTCATGTCTTTGCGATAGTTCTTCCTACTCAACCGCCTGGCGGAAATAAATCTAAATCTTCCACTCACCATCGTCCCAATTGACATCGTATAAATAACACCTGAAACTTTCTTAACTGGAAAGGATAAATTCTTTCCATTTTCACCAg                                                                                                                           >  4:322445/1‑149 (MQ=255)                     aTGTCTTTGCGATAGTTCTTCCTACTCAACCGCCTGGCGGAAATAAATCTAAATCTTCCACTCACCATCGTCCCAATTGACATCGTATAAATAACACCTGAAACTTTCTTAACTGGAAAGGATAAATTCTTTCCATTTTCACCAGCGTc                                                                                                                       <  7:196941/149‑1 (MQ=255)                      tGTCTTTGCGATAGTTCTTCCTACTCAACCGCCTGGCGGAAATAAATCTAAATCTTCCACTCACCATCGTCCCAATTGACATCGTATAAATAACACCTGAAACTTTCTTAACTGGAAAGGATAAATTCTTTCCATTTTCACCAGCGTCg                                                                                                                      <  2:268766/149‑1 (MQ=255)                       gTCTTTGCGATAGTTCTTCCTACTCAACCGCCTGGCGGAAATAAATCTAAATCTTCCACTCACCATCGTCCCAATTGACATCGTATAAATAACACCTGAAACTTTCTTAACTGGAAAGGATAAATTCTTTCCATTTTCACCAGCGTc                                                                                                                       >  6:253780/1‑147 (MQ=255)                       gTCTTTGCGATAGTTCTTCCTACTCAACCGCCTGGCGGAAATAAATCTAAATCTTCCACTCACCATCGTCCCAATTGACATCGTATAAATAACACCTGAAACTTTCTTAACTGGAAAGGATAAATTCTTTCCATTTTCACCAGCGTCga                                                                                                                     >  1:346252/1‑149 (MQ=255)                          tttGCGATAGTTCTTCCTACTCAACCGCCTGGCGGAAATAAATCTAAATCTTCCACTCACCATCGTCCCAATTGACATCGTATAAATAACACCTGAAACTTTCTTAACTGGAAAGGATaaa                                                                                                                                              >  5:267258/1‑121 (MQ=255)                          tttGCGATAGTTCTTCCTACTCAACCGCCTGGCGGAAATAAATCTAAATCTTCCACTCACCATCGTCCCAATTGACATCGTATAAATAACACCTGAAACTTTCTTAACTGGAAAGGATaaa                                                                                                                                              <  6:267258/121‑1 (MQ=255)                          tttGCGATAGTTCTTCCTACTCAACCGCCTGGCGGAAATAAATCTAAATCTTCCACTCACCATCGTCCCAATTGACATCGTATAAATAACACCTGAAACTTTCTTAACTGGAAAGGATAAATTCTTTCCATTTTCACCAGCGTCGAGAt                                                                                                                  <  2:346252/149‑1 (MQ=255)                           ttGCGATAGTTCTTCCTACTCAACCTCCTGGCGTAAATAAATCTAAATCTTCCACTCACCATCGTCCCAATTGACATCGTATAAATAACACCTGAAACTTTCTTAACTGGAAAGGATAAATTCTTTCGATTTTCACCAGCGTCGAGAt                                                                                                                  <  8:22259/148‑1 (MQ=255)                            tGCGATAGTTCTTCCTACTCAACCGCCTGGCGTAAATAAATCTAAATCTTCCACTCACCATCGTCCCAATTGACATCGTATAAATAACACCTGAAACTTTCTTAACTGGAAAGGATAAATTCTTTCCATTTTCACCAGCGTCgaga                                                                                                                   <  7:229668/146‑1 (MQ=255)                             gCGATAGTTCTTCCTACTCAACCGCCTGGCGGAAATAAATCTAAATCTTCCACTCACCATCGTCCCAATTGACATCGTATAAATAACACCTGAAACTTTCTTAACTGGAAAGGATAAATTCTTTCCATTTTCACCAGCGTCGAGATCa                                                                                                                <  4:82592/148‑1 (MQ=255)                               gATAGATCTTCCTACTCAACCGCCTGCCGGAAATAAATCTAAATCTTCCACTCACCATCGTCCCAATTCACATCGTATAAAAAACACCTGAAAATTTCTTAACTGGAAAGCATAAATTCTTACCATATTGACAAGAGTCGAGAACAGa                                                                                                              >  8:75897/1‑148 (MQ=255)                                         ccTACTCAACCGCCTGGCGGAAATAAATCTAAATCTTCCACTCACCATCGTCCCAATTGACATCGTATAAATAACACCTGAAACTTTCTTAACTGGAAAGGATAAATTTTTTCCATTTTCACCAGCGTCGAGATCAGAAAACATca                                                                                                      <  8:151409/146‑1 (MQ=255)                                         ccTACTCAACCGCCTGGCGGAAATAAATCTAAATCTTCCACTCACCATCGTCCCAATTGACATCGTATAAATAACACCTGAAACTTTCTTAACTGGAAAGGATAAATTCTTTCCATTTTCACCAGCGTCGAGATCAGAAAACATcaca                                                                                                    <  3:322445/148‑1 (MQ=255)                                         ccTACTCAACCGCCTGGCGGAAATAAATCTAAATCTTCCACTCACCATCGTCCCAATTGACATCGTATAAATAACACCTGAAACTTTCTTAACTGGAAAGGATAAATTCTTTCCATTTTCACCAGCGTCGAGATCAGAAAACATca                                                                                                      <  5:247054/146‑1 (MQ=255)                                           tACTCAACCGCCTGGCGGAAATAAATCTAAATCTTCCACTCACCATCGTCCCAATTGACATCGTATAAATAACACCTGAAACTTTCTTAACTGGAAAGGATAAATTCTTTCCATTTTCACCAGCGTCGAGATCAGAAAACATCACAGTg                                                                                                 >  1:78832/1‑149 (MQ=255)                                           tACTCAACCGCCTGGCGGAAATAAATCTAAATCTTCCACTCACCATCGTCCCAATTGACATCGTATAAATAACACCTGAAACTTTCTTAACTGGAAAGGATAAATTCTTTCCATTTTCACCAGCGTCGAGATCAGAAAACATCACAGTg                                                                                                 >  4:151837/1‑149 (MQ=255)                                              tCAACCGCCTGGCGGAAATAAATCTAAATCTTCCACTCACCATCGTCCCAATTGACATCGTATAAATAACACCTGAAACTTTCTTAACTGGAAAGGATAAATTCTTTCCATTTTCACCAGCGTCgag                                                                                                                    >  5:76628/1‑127 (MQ=255)                                              tCAACCGCCTGGCGGAAATAAATCTAAATCTTCCACTCACCATCGTCCCAATTGACATCGTATAAATAACACCTGAAACTTTCTTAACTGGAAAGGATAAATTCTTTCCATTTTCACCAGCGTCgag                                                                                                                    <  6:76628/127‑1 (MQ=255)                                              tCAACCGCCTGGCGGAAATAAATCTAAATCTTCCACTCACCATCGTCCCAATTGACATCGTATAAATAACACCTGAAACTTTCTTAACTGGAAAGGATAAATTCTTTCCATTTTCACCAGCGTCGAGATCAGAAAACATCACAGTgcgg                                                                                              >  4:102807/1‑149 (MQ=255)                                              tCAACCGCCTCGCGGAAATAAATCTAAATCTTCCACTCACCATCGTCCCAATTGACATCGTATAAATAACACATGAAACTTTCTTAACTGGAAAGGATAAATTCTTTCCATTTTCACCAGCGTCGAGATAAGAAACCATCACAGTgcgc                                                                                              >  8:283638/1‑148 (MQ=255)                                                  ccGCCTGGCGGAAATAAATCTAAATCTTCCACTCACCATCGTCCCAATTGACATCGTATAAATAACACCTGAAACTTTCTTAACTGGAAAGGATAAATTCTTTCCATTTTCACCAGCGTCGAGATCAGAAAACATCACAGTGCGGCGAc                                                                                          >  5:208348/1‑149 (MQ=255)                                                        ggCGGAAATAAATCTAAATCTTCCACTCACCATCGTCCCAATTGACATCGTATAAATAACACCTGAAACTTTCTTAACTGGAAAGGATAAATTCtt                                                                                                                                         <  4:301847/96‑1 (MQ=255)                                                        ggCGGAAATAAATCTAAATCTTCCACTCACCATCGTCCCAATTGACATCGTATAAATAACACCTGAAACTTTCTTAACTGGAAAGGATAAATTCtt                                                                                                                                         >  3:301847/1‑96 (MQ=255)                                                                  aatctaaatctTCCACTCACCATCGTCCCAATTGACATCGTATAAATAACACCTGAAACTTTCTTAACTGGAAAGGATAAATTCTTTCCATTTTCACCAGCGTCGAGATCAGAAAACATCACAGTGCGGCGACCATCTCTCGGATGAg                                                                           <  4:154444/148‑1 (MQ=255)                                                                      taaatctTCCACTCACCATCGTCCCAATTGACATCGTATAAATAACACCTGAAACTTTCTTAACTGGAAAGGATAAATTCTTTCCATTTTCACCAGCGTCGAGATCAGAAAACATCACAGTGCGGCGACCATCTCTCGGATGAGGACa                                                                       >  8:180679/1‑148 (MQ=255)                                                                        aatctTCCACTCACCATCGTCCCAATTGACATCGTATAAATAACACCTGAAACTTTCTTAACTGGAAAGGATAAATTCTTTCCATTTTCACCAGCGTCGAGATCAGAAAACATCACAGTGCGGCGACCATCTCTCGGATGAGGACAAAc                                                                    >  1:241056/1‑149 (MQ=255)                                                                           ctTCCACTCACCATCGTCCCAATTGACATCGTATAAATAACACCTGAAACTTTCTTAACTGGAAAGGATAAATTCTTTCCATTTTCACCAGCGTCGAGATCAGAAAACATCACAGTGCGGCGACCATCTCTCGGATGAGGACAAACGAc                                                                 >  6:239919/1‑149 (MQ=255)                                                                            ttCCACTCACCATCGTCCCAATTGACATCGTATAAATAACACCTGAAACTTTCTTAACTGGAAAGGATAAATTCTTTCCATTTTCACCAGCGTCGAGATCAGAAAACATCACAGTGCGGCGACCATCTCTCGGATGAGGACAAACGACt                                                                >  8:194653/1‑149 (MQ=255)                                                                               cACTCACCATCGTCCCAATTGACATCGTATAAATAACACCTGAAACTTTCTTAACTGGAAAGGATAAATTCTTTCCATTTTCACCAGCGTCGAGATCAGAAAACATCACAGTGCGGCGACCATCTCTCGGATGAGGACAAACGACTGAt                                                             <  5:239919/149‑1 (MQ=255)                                                                                 cTCACCATCGTCCCAATTGACATCGTATAAATAACACCTGAAACTTTCTTAACTGGAAAGGATAAATTCTTTCCATTTTCACCAGCGTCGAGATCAGAAAACATCACAGTGCGGCGACCATCTCTCGGATGAGGACAAACGACTGATaa                                                           >  8:174603/1‑149 (MQ=255)                                                                                 cTCACCATCGTCCCAATTGACATCGTATAAATAACACCTGAAACTTTCTTAACTGGAAAGGATAAATTCTTTCCATTTTCACCAGCGTCGAGATCAGAAAACATCACAGTGCGGCGACCATCTCTCGGATGAGGACAAACGACTGATaa                                                           <  5:229292/149‑1 (MQ=255)                                                                                    aCCATCGTCCCAATTGACATCGTATAAATAACACCTGAAACTTTCTTAACTGGAAAGGATAAATTCTTTCCATTTTCACCAGCGTCGAGATCAGAAAACATCACAGTGCGGCGACCATCTCTCGGATGAGGAc                                                                        >  7:134777/1‑133 (MQ=255)                                                                                    aCCATCGTCCCAATTGACATCGTATAAATAACACCTGAAACTTTCTTAACTGGAAAGGATAAATTCTTTCCATTTTCACCAGCGTCGAGATCAGAAAACATCACAGTGCGGCGACCATCTCTCGGATGAGGAc                                                                        >  7:129500/1‑133 (MQ=255)                                                                                    aCCATCGTCCCAATTGACATCGTATAAATAACACCTGAAACTTTCTTAACTGGAAAGGATAAATTCTTTCCATTTTCACCAGCGTCGAGATCAGAAAACATCACAGTGCGGCGACCATCTCTCGGATGAGGAc                                                                        <  8:134777/133‑1 (MQ=255)                                                                                    aCCATCGTCCCAATTGACATCGTATAAATAACACCTGAAACTTTCTTAACTGGAAAGGATAAATTCTTTCCATTTTCACCAGCGTCGAGATCAGAAAACATCACAGTGCGGCGACCATCTCTCGGATGAGGACAAACGACTGATAAt                                                          <  3:151837/147‑1 (MQ=255)                                                                                    aCCATCGTCCCAATTGACATCGTATAAATAACACCTGAAACTTTCTTAACTGGAAAGGATAAATTCTTTCCAATTTCACCAGCGTCGAGATCAGAAAACATCACAGTGCGGCGACCATCTCTCGGATGAGGAc                                                                        <  8:129500/133‑1 (MQ=255)                                                                                     ccATCGTCCCAATTGACATCGTATAAATAACACCTGAAACTTTCTTAACTGGAAAGGATAAATTCTTTCCATTTTCACCAGCGTCGAGATCAGAAAACATCACAGTGCGGCGACCATCTCTCGGATGAGGACAAACGACTGATAATGTa                                                       <  2:241056/149‑1 (MQ=255)                                                                                      cATCGTCCCAATTGACATCGTATAAATAACACCTGAAACTTTCTTAACTGGAAAGGATAAATTCTTTCCATTTTCACCAGCGTCGAGATCAGAAAACATCACAGTGCGGCGACCATCTCTCGGATGAGGACAAACGACTGATAATGTAc                                                      >  1:58757/1‑149 (MQ=255)                                                                                          gTCCCAATTGACATCGTATAAATAACACCTGAAACTTTCTTAACTGGAAAGGATAAATTCTTTCCATTTTCACCAGCGTCGAGATCAGAAAACATCACAGTGCGGCGACCATCTCTCGGATGAGGACAAACGACTGATAATGTACCaaa                                                  <  3:102807/149‑1 (MQ=255)                                                                                             ccAATTGACATCGTATAAATAACACCTGAAACTTTCTTAACTGGAAAGGATAAATTCTTTCCATTTTCACCAGCGTCGAGATCAGAAAACATCACAGTGCGGCGACCATCTCTCGGATGAGGACAAACGACTGATAATGTACCAAACtt                                               >  6:179819/1‑149 (MQ=255)                                                                                                aTTGACATCGTATAAATAACACCTGAAACTTTCTTAACTGGAAAGGATAAATTCTTTCCATTTTCACCAGCGTCGAGATCAGAAAACATCACAGTGCGGCGACCATCTCTCGGATGAGGACAAACGACTGATAATGTACCAAACTTTTc                                            >  5:177872/1‑149 (MQ=255)                                                                                                 ttGACATCGTATAAATAACACCTGAAACTTTCTTAACTGGAAAGGATAAATTCTTTCCATTTTCACCAGCGTCGAGATCAGAAAACATCACAGTGCGGCGACCATCTCTCGGATGAGGACAAACGACTGATAATGTACCAAACTTTTcc                                           <  2:329618/149‑1 (MQ=255)                                                                                                    aCATCGTATAAATAACACCTGAAACTTTCTTAACTGGAAAGGATATATTCTTTCCATTTTCACCAGCGTCGAGATCAGAAAACATCACAGTGCGGCGACCATCTCTCGGATGAGGACAAACGACTGATAATGTACCAAACTTTTCCGa                                         <  6:210619/148‑1 (MQ=255)                                                                                                    aCATCGTATAAATAACACCTGAAACTTTCTTAACTGGAAAGGATAAATTCTTTCCATTTTCACCAGCGTCGAGATCAGAAAACATCACAGTGCGGCGACCATCTCTCGGATGAGGACAAACGACTGATAATGTACCAAACTTTTCCGAg                                        <  2:58757/149‑1 (MQ=255)                                                                                                     cATCGTATAAATAACACCTGAAACTTTCTTAACTGGAAAGGATAAATTCTTTCCATTTTCACCAGCGTCGAGATCAGAAAACATCACAGTGCGGCGACCATCTCTCGGATGAGGACAAACGACTGATAATGTACCAAACTTTTCCGAg                                        >  8:2529/1‑148 (MQ=255)                                                                                                      aTCGTATAAATAACACCTGAAACTTTCTTAACTGGAAAGGATAAATTCTTTCCATTTTCACCAGCGTCGAGATCAGAAAACATCACAGTGCGGCGACCATCTCTCGGATGAGGACAAACGACTGATAATGTACCAAACTTTTCCGAg                                        >  3:114189/1‑147 (MQ=255)                                                                                                       tCGTATAAATAACACCTGAAACTTTCTTAACTGGAAAGGATAAATTCTTTCCATTTTCACCAGCGTCGAGATCAGAAAACATCACAGTGCGGCGACCATCTCTCGGATGAGGACAAACGACTGATAATGTACCAAACTTTTCCGAGTAg                                     <  7:75897/149‑1 (MQ=255)                                                                                                         gTATAAATAACACCTGAAACTTTCTTAACTGGAAAGGATAAATTCTTTCCATTTTCACCAGCGTCGAGATCAGAAAACATCACAGTGCGGCGACCATCTCTCGGATGAGGACAAACGACTGATAATGTACCAAACTTTTCCGAGTAGCt                                   <  7:283638/149‑1 (MQ=255)                                                                                                          tataAATAACACCTGAAACTTTCTTAACTGGAAAGGATAAATTCTTTCCATTTTCACCAGCGTCGAGATCAGAAAACATCACAGTGCGGCGACCATCTCTCGGATGAGGACAAACGACTGATAATGTACCAAACTTTTCCGAGTAGCt                                   >  3:188225/1‑148 (MQ=255)                                                                                                           ataaataaCACCTGAAACTTTCTTAACTGGAAAGGATAAATTCTTTCCATTTTCACCAGCGTCGAGATCAGAAAACATCACAGTGCGGCGACCATATCTCGGATGAGGACAAACGACTGATAATGTACCAAACTTTTCCCAGTAGCTGa                                 >  1:280956/1‑149 (MQ=255)                                                                                                            taaataaCACCTGAAACTTTCTTAACTGGAAAGGATAAATTCTTTCCATTTTCACCAGCGTCGAGATCAGAAAACATCACAGTGCGGCGACCATCTCTCGGATGAGGCCAAACGACTGATAATGTACCAAACTTTTCCGAGTAGCTGa                                 <  2:78832/148‑1 (MQ=255)                                                                                                            taaataaCACCTGAAACTTTCTTAACTGGAAAGGATAAATTCTTTCCATTTTCACCAGCGTCGAGATCAGAAAACATCACAGTGCGGCGACCATCTCTCGGATGAGGACAAACGACTGATAATGTACCAAACTTTTCCGAGTAGCTGa                                 <  7:212012/148‑1 (MQ=255)                                                                                                                    acCTGAAACTTTCTTAACTGGAAAGGATAAATTCTTTCCATTTTCACCAGCGTCGAGATCAGAAAACATCACAGTGCGGCGACCATCTCTCGGATGAGGACAAACGACTGATAATGTACCAAACTTTTCCGAGTAGCTGACTACTTCGt                        <  8:321818/149‑1 (MQ=255)                                                                                                                       tGAAACTTTGTTATCTGTAAATGATTAATTCTTTCCATTTTCACCAGCTTCGAGATCAGAATACATCACAGTGAGGCGACCATCTCTCGGATGAGGACAAACGACTGATAATGTACCAAACTTTTCCTAGTAGCTgt                                 <  8:121502/137‑2 (MQ=255)                                                                                                                       tGAAACTTTCTTAACTGGAAAGGATAAATTCTTTCCATTTTCACCAGCGTc                                                                                                                       <  8:308119/51‑1 (MQ=255)                                                                                                                       tGAAACTTTCTTAACTGGAAAGGATAAATTCTTTCCATTTTCACCAGCGTc                                                                                                                       <  8:302788/51‑1 (MQ=255)                                                                                                                       tGAAACTTTCTTAACTGGAAAGGATAAATTCTTTCCATTTTCACCAGCGTc                                                                                                                       >  7:302788/1‑51 (MQ=255)                                                                                                                       tGAAACTTTCTTAACTGGAAAGGATAAATTCTTTCCATTTTCACCAGCGTc                                                                                                                       >  7:308119/1‑51 (MQ=255)                                                                                                                       tGAAACTTTCTTAACTGGAAAGGATAAATTCTTTCCATTTTCACCAGCGTCGAGATCAGAAAACATCACAGTGCGGTGACCATCTCTCGGATGAGGACAAACGACt                                                                <  1:216372/106‑1 (MQ=255)                                                                                                                       tGAAACTTTCTTAACTGGAAAGGATAAATTCTTTCCATTTTCACCAGCGTCGAGATCAGAAAACATCACAGTGCGGCGACCATCTCTCGGATGAGGACAAACGACTGATAATGTACCAAACTTTTCCGAGTAGCTGACTACTTCGTTa                      >  5:286840/1‑148 (MQ=255)                                                                                                                       tGAAACTTTCTTAACTGGAAAGGATAAATTCTTTCCATTTTCACCAGCGTCGAGATAAGAAAAAATCACAGTGCGGCGACCATCTCTCGGATGAGGACAAACGACt                                                                >  2:216372/1‑106 (MQ=255)                                                                                                                         aaaCTTTCTTAACTGGAAAGGATAAATTCTTTCCATTTTCACCAGCGTCGAGATCAGAAAACATCACAGTGCGGCGACCATCTCTCGGATGAGGACAAACGACTGATAATGTACCAAACTTTTCCGAGTAGCTGACTACTTCGTTaa                     <  6:177872/147‑1 (MQ=255)                                                                                                                         aaaCTTTCTTAACTGGAAAGGATAAATTCTTTCCATTTTCACCAGCGTCGAGATCAGAAAACATCACAGTGCGGCGACCATCTCTCGGATGAGGACAAACGACTGATAATGTACCAAACTTTTCCGAGTAGCTGACTACTTCGTTAAAg                   <  7:174603/149‑1 (MQ=255)                                                                                                                           aCTTTCTTAACTGGAAAGGATAAATTCTTTCCATTTTCACCAGCGTCGAGATCAGAAAACATCACAGTGCGGCGACCATCTCTCGGATGAGGACAAACGACTGATAATGTACCAAACTTTTCCGAGTAGCTGACTACTTCGTTAAAGCt                 <  6:286840/149‑1 (MQ=255)                                                                                                                                  aaaatGGAAAGAATTTATCCTTTCCATTTTCACCAGCGTCGAGATCAGAAAACATCACAGTGCGGCGACCATCTCTCGGATGAGGACAAACGACTGATAATGTACCAAACTTTTCCGAGTAg                                     >  4:336079/5‑122 (MQ=255)                                                                                                                                  aaaatGGAAAGAATTTATCCTTTCCATTTTCACCAGCGTCGAGATCAGAAAACATCACAGTGCGGCGACCATCTCTCGGATGAGGACAAACGACTGATAATGTACCAAACTTTTCCGAGTAg                                     <  3:336079/118‑1 (MQ=255)                                                                                                                                   aaCTGGAAAGGATAAATTCTTTCCATTTTCACCAGCGTCGAGATCAGAAAACATCACAGTGCGGCGACCATCTCTCGGATGAGGACAAACGACTGATAATGTACCAAACTTTTCCGAGTAGCTGac                                <  4:70392/126‑1 (MQ=255)                                                                                                                                   aaCTGGAAAGGATAAATTCTTTCCATTTTCACCAGCGTCGAGATCAGAAAACATCACAGTGCGGCGACCATCTCTCGGATGAGGACAAACGACTGATAATGTACCAAACTTTTCCGAGTAGCTGACTACTTCGTTAAAGCTTAATc            <  6:208348/146‑1 (MQ=255)                                                                                                                                   aaCTGGAAAGGATAAATTCTTTCCATTTTCACCAGCGTCGAGATCAGAAAACATCACAGTGCGGCCACCATCTCTCGGATGAGGACAAACGACTGATAATGTAACAAACTTTTCCCAGTAGCTGac                                >  3:65240/1‑126 (MQ=255)                                                                                                                                    aatGGAAAGAATTTATCCTTTCCATTTTCACCAGCGTCGAGATCAGAAAACATCACAGTGCGGCGACCATCTCTCGGATGAGGACAAACGACTGATAATGTACCAAACTTTTCCGAGTAGCTGa                                 >  6:61641/3‑124 (MQ=255)                                                                                                                                    aatGGAAAGAATTTATCCTTTCCATTTTCACCAGCGTCGAGATCAGAAAACATCACAGTGCGGCGACCATCTCTCGGATGAGGACAAACGACTGATAATGTACCAAACTTTTCCGAGTAGCTGa                                 <  5:61641/122‑1 (MQ=255)                                                                                                                                        gAAAGGATAAATTCTTTCCATTTTCACCAGCGTCGAGATCAGAAAACATCACAGTGCGGCGACCATCTCTCGGATGAGGACAAACGACTGATAATGTACCAAACTTTTCCGAGTAGCTGACTACTTCGTTAAAGCTTAATCCATGCtt     <  5:253780/148‑1 (MQ=255)                                                                                                                                          aaGGATAAATTCTTTCCATTTTCACCAGCGTCGAGATCAGAAAACATCACAGTTTGGCGACCATCTCTCGGATGATGACAAACGACTGATAATGTACCAAACTTTTCCGAGTAGCTGACTACTTCGTTAAAGCTTAATCCATGCTtgat  <  3:30629/149‑1 (MQ=255)                                                                                                                                              aTAAATTCTTTCCATTTTCACCAGCGTCGAGATCAGAAAACATCACAGTGCGGCGACCATCTCTCGGATGAGGACAAACGACTGATAATGTACCAAACTTTTcc                                           <  6:336542/104‑1 (MQ=255)                                                                                                                                              aTAAATTCTTTCCATTTTCACCAGCGTCGAGATCAGAAAACATCACAGTGCGGCGACCATCTCTCGGATGAGGACAAACGACTGATAATGTACCAAACTTTTcc                                           >  5:336542/1‑104 (MQ=255)                                                                                                                                                 |                                                                                                                                              TACCCTTAAAAGCCTGCTTCATGTCTTTGCGATAGTTCTTCCTACTCAACCGCCTGGCGGAAATAAATCTAAATCTTCCACTCACCATCGTCCCAATTGACATCGTATAAATAACACCTGAAACTTTCTTAACTGGAAAGGATAAATTCTTTCCATTTTCACCAGCGTCGAGATCAGAAAACATCACAGTGCGGCGACCATCTCTCGGATGAGGACAAACGACTGATAATGTACCAAACTTTTCCGAGTAGCTGACTACTTCGTTAAAGCTTAATCCATGCTTGAT  >  NC_002947/5049865‑5050150

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑