Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,070,516 A→T 7.6% L124Q (CTG→CAG)  PP_4466 ← TauD/TfdA family dioxygenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,070,5160AT 7.6% 193.8 / 5.4 66L124Q (CTG→CAG) PP_4466TauD/TfdA family dioxygenase
Reads supporting (aligned to +/- strand):  ref base A (27/34);  new base T (2/3);  total (29/37)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.40e-01

CGCCGCGTCATACACGCTCTGGAAGAAGATCTGCCGGCCTTTGACTGCGTCCAGTAGATAGGACGGCAGGGTCTCGTAAGCCTGATACATCGATACCCAATAGGTGTCACCACCAGTGGGCGGAAGTGCAATGGAGCGCAGCAGTGCTCCAGCTGGTGGACGCTCGTAGAACCAGGTATCGGTGTGCCAGGTCGCTTCGCTGTTACCCAAAGCACCCGATGGCTTACCGTCTTTAGTAGTGTTAGCAATAACCAAAATTTCTTTTTGCGTTGGATGAGCGCCCTCTTG  >  NC_002947/5070374‑5070661
                                                                                                                                              |                                                                                                                                                 
cgccgcGTCATACACGCTCTGGAAGAAGATCTGCCGGCCTTTGACTGCGTCCAGTAGATAGGACGGCAGGGTCTCGTAAGCCTGATACATCGATACCCAATAGGTGTCACCACCAGTGGGCGGAAGTGCAATGGAGCGCAGCAGTGCTc                                                                                                                                             <  6:180683/149‑1 (MQ=255)
    gcgTCATACACGCTCTGGAAGAAGATCTGCCGGCCTTTGACTGCGTCCAGTAGATAGGACGGCAGGGTCTCGTAAGCCTGATACATCGATACCCAATAGGTGTCACCACCAGTGGGCGGAAGTGCAATGGAGCGCAGCAGTGCTCCAGc                                                                                                                                         <  7:255707/149‑1 (MQ=255)
    gcgTCATACACGCTCTGGAAGAAGATCTGCCGGCCTTTGACTGCGTCCAGTAGATAGGACGGCAGGGTCTCGTAAGCCTGATACATCGATACCCAATAGGTGTCACCACCAGTGGGCGGAAGTGCAATGGAGCACTGCTGCGCTCCatt                                                                                                                                         <  5:159362/149‑3 (MQ=255)
     cgTCATACACGCTCTGGAAGAAGATCTGCCGGCCTTTGACTGCGTCCAGTAGATAGGACGGCAGGGTCTCGTAAGCCTGATACATCGATACCCAATAGGTGTCACCACCAGTGGGCGGAAGTGCAATGGAGCGCAGCAGTGCTCCAg                                                                                                                                          >  1:37103/1‑147 (MQ=255)
     cgTCATACACGCTCTGGAAGAAGATCTGCCGGCCTTTGACTGCGTCCAGTAGATAGGACGGCAGGGTCTCGTAAGCCTGATACATCGATACCCAATAGGTGTCACCACCAGTGGGCGGAAGTGCAATGGAGCGCAGCAGTGCTCCAg                                                                                                                                          <  2:37103/147‑1 (MQ=255)
     cgTCATACACGCTCTGGAAGAAGATCTGCCGGCCTTTGACTGCGTCCAGTAGATAGGACGGCAGGGTCTCGTAAGCCTGATACATCGATACCCAATAGGTGTCACCACCAGTGGGCGGAAGTGCAATGGAGCGCAGCAGTGCTCCAGCt                                                                                                                                        >  8:188038/1‑149 (MQ=255)
         aTACACGCTCTGGAAGAAGATCTGCCGGCCTTTGACTGCGTCCAGTAGATAGGACGGCAGGGTCTCGTAAGCCTGATACATCGATACCCAATAGGTGTCACCACCAGTGGGCGGAAGTGCAATGGAGCGCAGCAGTGCTCCAGCtggtg                                                                                                                                    <  5:332690/149‑1 (MQ=255)
         aTACACGCTCTGGAAGAAGATCTGCCGGCCTTTGACTGCGTCCAGTAGATAGGACGGCAGGGTCTCGTAAGCCTGATACATCGATACCCAATAGGTGTCACCACCAGTGGGCGGAAGTGCAATGGAGCGCAGCAGTGCTCCAGCtggt                                                                                                                                     <  1:145731/148‑1 (MQ=255)
         aTACACGCTCTGGAAGAAGATCTGCCGGCCTTTGACTGCGTCCAGTAGATAGGACGGCAGGGTCTCGTAAGCCTGATACATCGATACCCAATAGGTGTCACCACCAGTGGGCGGAAGTGCAATGGAGCGCAGCAGTGCTCCAGCtggt                                                                                                                                     <  2:98268/148‑1 (MQ=255)
          tACACGCTCTGGAAGAAGATCTGCCGGCCTTTGACTGCGTCCAGTAGATAGGACGGCAGGGTCTCGTAAGCCTGATACATCGATACCCAATAGGTGTCACCACCAGTGGGCGGAAGTGCAATGGAGCGCAGCAGTGCTCCAGCtggtg                                                                                                                                    >  5:175504/1‑148 (MQ=255)
           acacGCTCTGGAAGAAGATCTGCCGGCCTTTGACTGCGTCCAGTAGATAGGACGGCAGGGTCTCGTAAGCCTGATACATCGATACCCAATAGGTGTCACCACCAGTGGGCGGAAGTGCAATGGAGCACTGCTGCGCTCCatt                                                                                                                                         >  5:49887/1‑140 (MQ=255)
           acacGCTCTGGAAGAAGATCTGCCGGCCTTTGACTGCGTCCAGTAGATAGGACGGCAGGGTCTCGTAAGCCTGATACATCGATACCCAATAGGTGTCACCACCAGTGGGCGGAAGTGCAATGGAGCACTGCTGCGCTCCatt                                                                                                                                         <  6:49887/142‑3 (MQ=255)
            cacGCTCTGGAAGAAGATCTGCCGGCCTTTGACTGCGTCCAGTAGATAGGACGGCAGGGTCTCGTAAGCCTGATACATAGATACCCAATAGGTGTCACCACCACTGGGCGGAAGTGCAATGGAGCGCAGCAGTGCTCCAGCTGGTGGAc                                                                                                                                 >  6:246470/1‑149 (MQ=255)
             acGCTCTGGAAGAAGATCTGCCGGCCTTTGACTGCGTCCAGTAGATAGGACGGCAGGGTCTCGTAAGCCTGATACATCGATACCCAATAGGTGTCACCACCAGTGGGCGGAAGTGCAATGGAGCGCAGCAGTGCTCCAGCTGGTGGACg                                                                                                                                >  2:365641/1‑149 (MQ=255)
             acGCTCTGGAAGAAGATCTGCCGGCCTTTGACTGCGTCCAGTAGATAGGACGGCAGGGTCTCGTAAGCCTGATACATCGATACCCAATAGGTGTCACCACCAGTGGGCGGAAGTGCAATGGAGCGCAGCAGTGCTCCAGCTGGTGGACg                                                                                                                                >  6:238033/1‑149 (MQ=255)
                ctctGGAAGAAGATCTGCCGGCCTTTGACTGCGTCCAGTAGATAGGACGGCAGGGTCTCGTAAGCCTGATACATCGATACCCAATAGGTGTCACCACCAGTGGGCGGAAGTGCAATGGAGCGCAGCAGTGCTCCAGCTGGTGGACGCTc                                                                                                                             <  7:204932/149‑1 (MQ=255)
                     gaagaaGATCTGCCGGCCTTTGACTGCGTCCAGTAGATAGGACGGCAGGGTCTCGTAAGCCTGATACATCGATACCCAATAGGTGTCACCACCAGTGGGCGGAAGTGCAATGGAGCGCAGCAGTGCTCCAGCTGGTGGACGCTCGTa                                                                                                                          <  3:172827/147‑1 (MQ=255)
                     gaagaaGATCTGCCGGCCTTTGACTGCGTCCAGTAGATAGGACGGCAGGGTCTCGTAAGCCTGATACATCGATACCCAATAGGTGTCACCACCAGTGGGCGGAAGTGCAATGGAGCGCAGCAGTGCCCCAGCTGGTGGACGCTc                                                                                                                             <  8:121271/144‑1 (MQ=255)
                     gaagaaGATCTGCCGGCCTTTGACTGCGTCCAGTAGATAGGACGGCAGGGTCTCGTAAGCCTGATACATCGATACCCAATAGGTGTCACCACCAGTGGGCGGAAGTGCAATGGAGCGCAGCAGTGCCCCAGCTGGTGGACGCTc                                                                                                                             >  7:121271/1‑144 (MQ=255)
                       agaagaTCTGCCGGCCTTTGACTGCGTCCAGTAGATAGGACGGCAGGGTCTCGTAAGCCTGATACATCGATACCCAATAGGTGTCACCACCAGTGGGCGGAAGTGCAATGGAGCGCAGCAGTGCTCCAGCTGGTGGACGCTCGTAGAAc                                                                                                                      >  2:233983/1‑149 (MQ=255)
                        gaagaTCTGCCGGCCTTTGACTGCGTCCAGTAGATAGGACGGCAGGGTCTCGTAAGCCTGATACATCGATACCCAATAGGTGTCACCACCAGTGGGCGGAAGTGCAATGGAGCGCAGCAGTGCTCCAGCTGGTGGACGCTCGTAGAAcc                                                                                                                     <  3:67456/149‑1 (MQ=255)
                          agaTCTGCCGGCCTTTGACTGCGTCCAGTAGATAGGACGGCAGGGTCTCGTAAGCCTGATACATCGATACCCAATAGGTGTCACCACCAGTGGGCGGAAGTGCAATGGAGCGCAGCAGTGCTCCAGCTGGTGGACGCTCGTAGAACCAg                                                                                                                   <  5:183185/149‑1 (MQ=255)
                          agaTCTGCCGGCCTTTGACTGCGTCCAGTAGATAGGACGGCAGGGTCTCGTAAGCCTGATACATCGATACCCAATAGGTGTCACCACCAGTGGGCGGAAGTGCAATGGAGCGCAGCAGTGCTCCAGCTGGTGGACGCTCGTAGAACCAg                                                                                                                   <  1:365641/149‑1 (MQ=255)
                                  cGGCCTTTGACTGCGTCCAGTAGATAGGACGGCAGGGTCTCGTAAGCCTGATACATCGATACCCAATAGGTGTCACCACCAGTGGGCGGAAGTGCAATGGAGCGCAGCAGTGCTCCAGCTGGTGGACGCTCGTAGAACCAGGTATCGgt                                                                                                           <  7:188038/149‑1 (MQ=255)
                                  cGGCCTTTGACTGCGTCCAGTAGATAGGACGGCAGGGTCTCGTAAGCCTGATACATCGATACCCAATAGGTGTCACCACCAGTGGGCGGAAGTGCAATGGAGCGCAGCAGTGCTCCAGCTGGTGGACGCTCGTAGAACCAGGTATCGgt                                                                                                           >  1:184970/1‑149 (MQ=255)
                                       tttGACTGCGTCCAGTAGATAGGACGGCAGGGTCTCGTAAGCCTGATACATCGATACCCAATAGGTGTCACCACCAGTGGGCGGAAGTGCAATGGAGCGCAGCAGTGCTCCAGCTGGTGGACGCTCGTAGAACCAGGTATCg                                                                                                             >  3:165915/1‑142 (MQ=255)
                                       tttGACTGCGTCCAGTAGATAGGACGGCAGGGTCTCGTAAGCCTGATACATCGATACCCAATAGGTGTCACCACCAGTGGGCGGAAGTGCAATGGAGCGCAGCAGTGCTCCAGCTGGTGGACGCTCGTAGAACCAGGTATCg                                                                                                             <  4:165915/142‑1 (MQ=255)
                                                    aGTAGATAGGACGGCAGGGTCTCGTAAGCCTGATACATCGATACCCAATAGGTGTCACCACCAGTGGGCGGAAGTGCAATGGAGCGCAGCAGTGCTCCAGCTGGTGGACGCTCGTAGAACCAGGTATCGGTGTGCCAGGtcgcttcgct                                                                                         <  5:380725/149‑1 (MQ=255)
                                                                ggCAGGGTCTCGTAAGCCTGATACATCGATAGCATATAGGTGTGACCTCCTGTGTGCGGAAGTGCAATTGAGCGCAGCAGTGCTCCAGCTGGTGGACGCTTGTAGAACCAGGTATCGGTGTGCCAGGTCGCTTCGCTGTTACCCAAAGc                                                                             <  5:238033/149‑1 (MQ=255)
                                                                   aGGGTCTCGTAAGCCTGATACATCGATACCCAATAGGTGTCACCACCAGTGGGCGGAAGTGCAATGGAGCGCAGCAGTGCTCCAGCTGGTGGACGCTCGTAGAACCAGGTATCGGTGTGCCAGGTCGCTTCGTTGTTACCCAAAGCAcc                                                                          <  1:343286/149‑1 (MQ=255)
                                                                    gggTCTCGTAAGCCTGATACATCGATACCCAATAGGTGTCACCACCAGTGGGCGGAAGTGCAATGGAGCGCAGCAGTGCTCCAGCTGGTGGACGCTCGTAGAACCAGGTATCGGTGTGCCAGGTCGCTTCGCTGTTACCCAAAGCAccc                                                                         >  3:5400/1‑149 (MQ=255)
                                                                           gTAAGCCTGATACATCGATACCCAATAGGTGTCACCACCAGTGGGCGGAAGTGCAATGGAGCGCAGCAGTGCTCCAGCTGGTGGACGCTCGTAGAACCAGGTATCGGTGTGCCAGGTCGCTTCGCTGTTACCCAAAGCACCCGATGGCt                                                                  >  8:51631/1‑149 (MQ=255)
                                                                              aGCCTGATACATCGATACCCAATAGGTGTCACCACCAGTGGGCGGAAGTGCAATGGAGCGCAGCAGTGCTCCAGCTGGTGGACGCTCGTAGAACCAGGTATCGGTGTGCCAGGTCGCTTCGCTGTTACCCAAAGCACCCGATGGCTTa                                                                <  1:146691/148‑1 (MQ=255)
                                                                               gCCTGATACATCGATACCCAATAGGTGTCACCACCAGTGGGCGGAAGTGCAATGGAGCGCAGCAGTGCTCCAGCTGGTGGACGCTCGt                                                                                                                           >  5:15737/1‑88 (MQ=255)
                                                                               gCCTGATACATCGATACCCAATAGGTGTCACCACCAGTGGGCGGAAGTGCAATGGAGCGCAGCAGTGCTCCAGCTGGTGGACGCTCGt                                                                                                                           <  6:15737/88‑1 (MQ=255)
                                                                                    aTACATCGATACCCAATAGGTGTCACCACCAGTGGGCGGAAGTGCAATGGAGCGCAGCAGTGCTCCAGCTGGTGGACGCTCGTAGAACCAGGTATCGGTGTGCCAGGTCGCTTCGCTGTTACCCAAAGCACCCGATGGCTTACCGTCtt                                                         <  1:233983/149‑1 (MQ=255)
                                                                                    aTACATCGATACCCAATAGGTGTCACCACCAGTGGGCGGAAGTGCAATGGAGCGCAGCAGTGCTCCAGCTGGTGGACGCTCGTAGAACCAGGTATCGGTGTGCCAGGTCGCTTCGCTGTTACCCAAAGCACCCGATGGCTTACCGTCt                                                          <  4:5400/148‑1 (MQ=255)
                                                                                      aCATCGATACCCAATAGGTGTCACCACCAGTGGGCGGAAGTGCAATGGAGCGCAGCAGTGCTCCAGCTGGTGGACGCTCGTAGAACCAGGTATCGGTGTGCCAGGTCGCTTCGCTGTTACCCAAAGCACCCGAt                                                                      >  4:80821/1‑134 (MQ=255)
                                                                                      aCATCGATACCCAATAGGTGTCACCACCAGTGGGCGGAAGTGCAATGGAGCGCAGCAGTGCTCCAGCTGGTGGACGCTCGTAGAACCAGGTATCGGTGTGCCAGGTCGCTTCGCTGTTACCCAAAGCACCCGATGGCTTACCGTCtt                                                         >  8:186751/1‑147 (MQ=255)
                                                                                      aCATCGATACCCAATAGGTGTCACCACCAGTGGGCGGAAGTGCAATGGAGCGCAGCAGTGCTCCAGCTGGTGGACGCTCGTAGAACCAGGTAGCGGTGTGCCAGGTCGCTTCGCTGTTACCCAAAGCACCCGAt                                                                      <  3:80821/134‑1 (MQ=255)
                                                                                       cATCGATACCCAATAGGTGTCACCACCAGTGGGCGGAAGTGCAATGGAGCGCAGCAGTGCTCCAGCTGGTGGACGCTCGTAGAACCAGGTATCGGTGTGCCAGGTCGCTTCGCTGTTACCCAAAGCACCCGATGGCTTACCGTCTTta                                                       <  2:184970/148‑1 (MQ=255)
                                                                                       cATCGATACCCAATAGGTGTCACCACCAGTGGGCGGAAGTGCAATGGAGCGCAGCAGTGCTCCAGCTGGTGGACGCTCGTAGAACCAGGTATCGGTGTGCCAGGTCGCTTCGCTGTTACCCAAAGCACCCGATGGCTTACCGTCTTta                                                       <  5:355605/148‑1 (MQ=255)
                                                                                        aTCGATACCCAATAGGTGTCACCATCAGTGGGCGGAAGTGCAATGGAGCGCAGCAGTGCTCCAGCTGGTGGACGCTCGTAGAACCAGGTATCGGTGTGCCAGGTCGCTTCGCTGTTACCCAAAGCACCCGATGGCTTACCGTCTTtag                                                      >  7:234344/1‑148 (MQ=255)
                                                                                             tACCCAATAGGTGTCACCACCAGTGGGCGGAAGTGCAATGGAGCGCAGCAGTGCTCCAGCTGGTGGACGCTCGTAGAACCAGGTATCGGTGTGCCAGGTCGCTTCGCTGTTACCCAAAGCACCCGATGGCTTACCGTCTTTAGTAgtgt                                                <  1:74113/149‑1 (MQ=255)
                                                                                             tACCCAATAGGTGTCACCACCAGTGGGCGGAAGTGCAATGGAGCGCAGCAGTGCTCCAGCTGGTGGACGCTCGTAGAACCAGGTATCGGTGTGCCAGGTCGCTTCGCTGTTACCCAAAGCACCCGATGGCTTACCGTCTTTAGTAgtgt                                                <  5:246470/149‑1 (MQ=255)
                                                                                             tACCCAATAGGTGTCACCACCAGTGGGCGGAAGTGCAATGGAGCGCAGCAGTGCTCCAGCTGGTGGACGCTCGTAGAACCAGGTATCGGTGTGCCAGGTCGCTTCGCTGTTACCCAAAGCACCCGATGGCTTACCGTCTTTAGTAgtgt                                                <  6:175504/149‑1 (MQ=255)
                                                                                                ccAATAGGTGTCACCACCAGTGGGCGGAAGTGCAATGGAGCGCAGCAGTGCTCCAGCtggtgg                                                                                                                                   >  7:158230/1‑63 (MQ=255)
                                                                                                ccAATAGGTGTCACCACCAGTGGGCGGAAGTGCAATGGAGCGCAGCAGTGCTCCAGCtggtgg                                                                                                                                   <  8:158230/63‑1 (MQ=255)
                                                                                                ccAATAGGTGTCACCACCAGTGGGCGGAAGTGCAATGGAGCGCAGCAGTGCTCCAGCTGGTGGACGCTCGTAGAACCAGGTATCGGTGTGCCAGGTCGCTTCGCTGTTACCCAAAGCACCCGATGGCTTACCGTCTTTAGTAGTGTTAg                                             <  4:68897/149‑1 (MQ=255)
                                                                                                 cAATAGGTGTCACCACCAGTGGGCGGAAGTGCAATGGAGCGCAGCAGTGCTCCAGCTGGTGGACGCTCGTAGAACCAGGTATCGGTGTGCCAGGTCGCTTCGCTGTTACCCAAAGCACCCGATGGCTTACCGTCTTta                                                       <  3:159239/138‑1 (MQ=255)
                                                                                                 cAATAGGTGTCACCACCAGTGGGCGGAAGTGCAATGGAGCGCAGCAGTGCTCCAGCTGGTGGACGCTCGTAGAACCAGGTATCGGTGTGCCAGGTCGCTTCGCTGTTACCCAAAGCACCCGATGGCTTACCGTCTTta                                                       >  4:159239/1‑138 (MQ=255)
                                                                                                 cAATAGGTGTCACCACCAGTGGGCGGAAGTGCAATGGAGCGCAGCAGTGCTCCAGCTGGTGGACGCTCGTAGAACCAGGTATCGGTGTGCCAGGTCGCTTCGCTGTTACCCAAAGCACCCGATGGCTTACCGTCTTTAGTAGTGTTAg                                             >  2:291359/1‑148 (MQ=255)
                                                                                                  aaTAGGTGTCACCACCAGTGGGCGGAAGTGCAATGGAGCGCAGCAGTGCTCCAGCTGGTGGACGCTCGTAGAACCAGGTATCGGTGTGCCAGGTCGCTTCGCTGTTACCCAAAGCACCCGATGGCTTACCGTCTTtagta                                                    <  1:77559/140‑1 (MQ=255)
                                                                                                  aaTAGGTGTCACCACCAGTGGGCGGAAGTGCAATGGAGCGCAGCAGTGCTCCAGCTGGTGGACGCTCGTAGAACCAGGTATCGGTGTGCCAGGTCGCTTCGCTGTTACCCAAAGCACCCGATGGCTTACCGTCTTtagta                                                    >  2:77559/1‑140 (MQ=255)
                                                                                                            accaccAGTGGGCGGAAGTGCAATGGAGCGCAGCAGTGCTCCAGCTGGTGGACGCTCGTAGAACCAGGTATCGGTGTGCCAGGTCGCTTCGCTGTTACCCAAAGCACCCGATGGCTTACCGTCTTTAGTAGTGTTAGCAATAACCaaaa                                 >  8:268713/1‑149 (MQ=255)
                                                                                                              caccaGTGGGCGGAAGTGCAATGGAGCGCAGCAGTGCTCCAGCTGGTGGACGCTCGTAGAACCAGGTATCGGTGTGCCAGGTCGCTTCGCTGTTACCCAAAGCACCCGATGGCTTACCGTCTTTAGTAGTGTTAGCAATAACCAAAAtt                               >  3:6394/1‑149 (MQ=255)
                                                                                                                      ggCGGAAGTGCAATGGAGCGCAGCAGTGCTCCAGCTGGTGGACGCTCGTAGAACCAGGTATCGGTGTGCCAGGTCGCTTCGCTGTTACCCAAAGCACCCGATGGCTTACCGTCTTTAGTAGTGTTAGCAATAACCAAAATTTCttttt                        <  8:234344/148‑1 (MQ=255)
                                                                                                                               gCAATGGAGCGCAGCAGTGCTCCAGCTGGTGGACGCTCGTAGAACCAGGTATCGGTGTGCCAGGTCGCTTCGCTGTTACCCAAAGCACCCGATGGCTTACCGTCTTTAGTAGTGTTAGCAATAACCAAAATTTCTTTTTGCGTTGGAt               <  7:186751/148‑1 (MQ=255)
                                                                                                                               gCAATGGAGCGCAGCAGTGCTCCAGCTGGTGGACGCTCGTAGAACCAGGTATCGGTGTGCCAGGTCGCTTCGCTGTTACCCAAAGCACCCGATGGCTTACCGTCTTTAGTAGTGTTAGCAATAACCAAAATTTCTTTTTGCGTTGGAt               >  8:146132/1‑148 (MQ=255)
                                                                                                                                cAATGGAGCGCAGCAGTGCTCCAGCTGGTGGACGCTCGTAGAACCAGGTATCGGTGTGCCAGGTCGCTTCGCTGTTACCCAAAGCACCCGATGGCTTACCGTCTTTAGTAGTGTTAGCAATAACCAAAATTTCTTTTTGCGTTGGAt               >  6:169135/1‑147 (MQ=255)
                                                                                                                                 aaTGGAGCGCAGCAGTGCTCCAGCTGGTGGACGCTCGTAGAACCAGGTATCGGTGTGCCTGGTCGCTTCGCTGTTACCCAAAGCACCCGATGGCTTACCGTCTTTAGTAGTGTTAGCAATAACCAAAATTTCTTTTTGCGTTGGATGAg            >  3:289008/1‑149 (MQ=255)
                                                                                                                                  ctGGAGCACTGCTGCGCTCCAGCTGGTGGACGCTCGTAGAACCAGGTATCGGTGTGCCAGGTCGCTTCGCTGTTACCCAAAGCACCCGATGGCTTACCGTCTTTAGTAGTGTTAGCAATAACCAAAATTTCttttt                        <  8:310864/135‑1 (MQ=255)
                                                                                                                                  ctGGAGCACTGCTGCGCTCCAGCTGGTGGACGCTCGTAGAACCAGGTATCGGTGTGCCAGGTCGCTTCGCTGTTACCCAAAGCACCCGATGGCTTACCGTCTTTAGTAGTGTTAGCAATAACCAAAATTTCttttt                        >  7:310864/2‑136 (MQ=255)
                                                                                                                                    ggAGCGCAGCAGTGCTCCAGCTGGTGGACGCTCGTAGAACCAGGTATCGGTGTGCCAGGTCGCTTCGCTGTTACCCAAAGCACCCGATGGCTTACCGTCTTTAGTAGTGTTAGCAATAACCAAAATTTCTTTTTGCGTTg                  <  6:340806/140‑1 (MQ=255)
                                                                                                                                    ggAGCGCAGCAGTGCTCCAGCTGGTGGACGCTCGTAGAACCAGGTATCGGTGTGCCAGGTCGCTTCGCTGTTACCCAAAGCACCCGATGGCTTACCGTCTTTAGTAGTGTTAGCAATAACCAAAATTTCTTTTTGCGTTg                  >  5:340806/1‑140 (MQ=255)
                                                                                                                                      aGCGCAGCAGTGCTCCAGCTGGTGGACGCTCGTAGAACCAGGTATCGGTGTGCCAGGTCGCTTCGCTGTTACCCAAAGCACCCGATGGCTTACCGTCTTTAGTAGTGTTAGCAATAACCAAAATTTCTTTTTGCGTTGGATGAGCGccc       >  1:109884/1‑149 (MQ=255)
                                                                                                                                           agcagTGCTCCAGCTGGTGGACGCTCGTAGAACCAGGTATCGGTGTGCCAGGTCGCTTCGCTGTTACCCAAAGCACCCGATGGCTTACCGTCTTTAGTAGTGTTAGCAATAACCAAAATTTCTTTTTGCGTTGGAt               >  5:128092/1‑136 (MQ=255)
                                                                                                                                           agcagTGCTCCAGCTGGTGGACGCTCGTAGAACCAGGTATCGGTGTGCCAGGTCGCTTCGCTGTTACCCAAAGCACCCGATGGCTTACCGTCTTTAGTAGTGTTAGCAATAACCAAAATTTCTTTTTGCGTTGGAt               <  6:128092/136‑1 (MQ=255)
                                                                                                                                           agcagTGCTCCAGCTGGTGGACGCTCGTAGAACCAGGTATCGGTGTGCCAGGTCGCTTCGCTGTTACCCAAAGCACCCGATGGCTTACCGTCTTTAGTAGTGTTAGCAATAACCAAAATTTCTTTTTGCGTTGGATGAGCGcc        >  3:129254/1‑143 (MQ=255)
                                                                                                                                           agcagTGCTCCAGCTGGTGGACGCTCGTAGAACCAGGTATCGGTGTGCCAGGTCGCTTCGCTGTTACCCAAAGCACCCGATGGCTTACCGTCTTTAGTAGTGTTAGCAATAACCAAAATTTCTTTTTGCGTTGGATGAGCGcc        <  4:129254/143‑1 (MQ=255)
                                                                                                                                           agcagTGCTCCAGCTGGTGGACGCTCGTAGAACCAGGTATCGGTGTGCCAGGTCGCTTCGCTGTTACCCAAAGCACCCGATGGCTTACCGTCTTTAGTAGTGTTAGCAATAACCAAAATTTCTTTTTGCGTTGGATGAGCGCCCTCTTg  >  8:14101/1‑149 (MQ=255)
                                                                                                                                              |                                                                                                                                                 
CGCCGCGTCATACACGCTCTGGAAGAAGATCTGCCGGCCTTTGACTGCGTCCAGTAGATAGGACGGCAGGGTCTCGTAAGCCTGATACATCGATACCCAATAGGTGTCACCACCAGTGGGCGGAAGTGCAATGGAGCGCAGCAGTGCTCCAGCTGGTGGACGCTCGTAGAACCAGGTATCGGTGTGCCAGGTCGCTTCGCTGTTACCCAAAGCACCCGATGGCTTACCGTCTTTAGTAGTGTTAGCAATAACCAAAATTTCTTTTTGCGTTGGATGAGCGCCCTCTTG  >  NC_002947/5070374‑5070661

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑