Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,097,141	A→C	7.2%	intergenic (+132/+160)	PP_mr66 → / ← acsA‑I	P6/acetyl‑CoA synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,097,141	0	A	C	7.2%	209.7 / 5.3	69	intergenic (+132/+160)	PP_mr66/acsA‑I	P6/acetyl‑CoA synthetase
Reads supporting (aligned to +/- strand):  ref base A (27/37);  new base C (2/3);  total (29/40)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.64e-01

GCAAGAAATAGAACCTATAGACCTAGCAATTCCTGGGCCTATTATTGGCAAAGCCTTGTACGACGGCAAGTGCAGCGTGCCTTGATGCACAAAATGCTGACTCCGATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAAGCCCGCTCC  >  NC_002947/5096997‑5097275                                                                                                                                                 |                                                                                                                                        gcAAGAAATAGAACCTATAGACCTAGCAATTCCTGGGCCTATTATTGGCAAAGCCTTGTACGACGGCAAGTGCAGCGTGCCTTGATGCACAAAATGCTGACTCCGATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCt                                                                                                                                     <  5:201712/149‑1 (MQ=255) gcAAGAAATAGAACCTATAGACCTAGCAATTCCTGGGCCTATTATTGGCAAAGCCTTGTACGACGGCAAGTGCAGCGTGCCTTGATGCACAAAATGCTGACTCCGATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCt                                                                                                                                     >  1:271582/1‑149 (MQ=255) gcAAGAAATAGAACCTATAGACCTAGCAATTCCTGGGCCTATTATTGGCAAAGCCTTGTACGACGGCAAGTGCAGCGTGCCTTGATGCACAAAATGCTGACTCCGATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCt                                                                                                                                     >  7:272370/1‑149 (MQ=255)   aaGAAATAGAACCTATAGACCTAGCAATTCCTGGGCCTATTATTGGCAAAGCCTTGTACGACGGCAAGTGCAGCGTGCCTTGATGCACAAAATGCTGACTCCGATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGCAcaggt                                                                                                                                   <  4:181995/149‑5 (MQ=255)   aaGAAATAGAACCTATAGACCTAGCAATTCCTGGGCCTATTATTGGCAAAGCCTTGTACGACGGCAAGTGCAGCGTGCCTTGATGCACAAAATGCTGACTCCGATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCtgt                                                                                                                                   <  6:138637/149‑1 (MQ=255)     gAAATAGAACCTATAGACCTAGCAATTCCTGGGCCTATTATTGGCAAAGCCTTGTACGACGGCAAGTGCAGCGTGCCTTGATGCACAAAATGCTGACTCCGATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGc                                                                                                                                 >  2:256044/1‑149 (MQ=255)       aaTAGAACCTATAGACCTAGCAATTCCTGGGCCTATTATTGGCAAAGCCTTGTACGACGGCAAGTGCAGCGTGCCTTGATGCACAAAATGCTGACTCCGATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCgg                                                                                                                               >  2:185192/1‑149 (MQ=255)        aTAGAACCTATAGACCTAGCAATTCCTGGGCCTATTATTGGCAAAGCCTTGTACGACGGCAAGTGCAGCGTGCCTTGATGCACAAAATGCTGACTCCGATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCggg                                                                                                                              <  8:272370/149‑1 (MQ=255)           gAACCTATAGACCTAGCAATTCCTGGGCCTATTATTGGCAAAGCCTTGTACGACGGCAAGTGCAGCGTGCCTTGATGCACAAAATGCTGACTCCGATGCACCGAAACGGGGCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGcc                                                                                                                           >  7:284216/1‑149 (MQ=255)              ccTATAGACCTAGCAATTCCTGGGCCTATTATTGGCAAAGCCTTGTACGACGGCAAGTGCAGCGTGCCTTGATGCACAAAATGCTGACTCCGATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGt                                                                                                                        <  1:236537/149‑1 (MQ=255)              ccTATAGACCTAGCAATTCCTGGGCCTATTATTGGCAAAGCCTTGTACGACGGCAAGTGCAGCGTGCCTTGATGCACAAAATGCTGACTCCGATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGt                                                                                                                        <  6:110738/149‑1 (MQ=255)               cTATAGACCTAGCAATTCCTGGGCCTATTATTGGCAAAGCCTTGTACGACGGCAAGTGCAGCGTGCCTTGATGCACAAAATGCTGACTCCGATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGCACAGGTTCgggg                                                                                                                             <  1:207646/143‑1 (MQ=255)               cTATAGACCTAGCAATTCCTGGGCCTATTATTGGCAAAGCCTTGTACGACGGCAAGTGCAGCGTGCCTTGATGCACAAAATGCTGACTCCGATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGCACAGGTTCgggg                                                                                                                             >  2:207646/1‑143 (MQ=255)                  taGACCTAGCAATTCCTGGGCCTATTATTGTCAAAGCCTTGTACGACGGCAAGTGCAGCGTGCCTTGATGCACAAAATGCTGACTCCGATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGa                                                                                                                      <  8:22525/147‑1 (MQ=255)                  taGACCTAGCAATTCCTGGGCCTATTATTGGCAAAGCCTTGTACGACGGCAAGTGCAGCGTGCCTTGATGCACAAAATGCTGACTCCGATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACa                                                                                                                    <  5:134651/149‑1 (MQ=255)                  taGACCTAGCAATTCCTGGGCCTATTATTAGCAAAGCCTTGTACGACGGCAAGTGCAGCGTGCCTTGATGCACAAAATGCTGACTCCGATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGa                                                                                                                      >  6:45711/1‑147 (MQ=255)                  taGACCTAGCAATTCCTGGGCCTATTATTAGCAAAGCCTTGTACGACGGCAAGGGCAGCGTGCCTTGATGCACAAAATGCTGACTCCGATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGa                                                                                                                      <  5:45711/147‑1 (MQ=255)                    gACCTAGCAATTCCTGGGCCTATTATTGGCAAAGCCTTGTACGACGGCAAGTGCAGCGTGCCTTGATGCACAAAATGCTGACTCCGATGCACCGAAACGGGGCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAg                                                                                                                   <  8:284216/148‑1 (MQ=255)                    gACCTAGCAATTCCTGGGCCTATTATTGGCAAAGCCTTGTACGACGGCAAGTGCAGCGTGCCTTGATGCACAAAATGCTGACTCCGATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAg                                                                                                                   <  4:230/148‑1 (MQ=255)                       cTAGCAATTCCTGGGCCTATTATTGGCAAAGCCTTGTACGACGGCAAGTGCAGCGTGCCTTGATGCACAAAATGCTGATTCCGATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGaa                                                                                                                 >  7:39658/1‑147 (MQ=255)                       cTAGCAATTCCTGGGCCTATTATTGGCAAAGCCTTGTACGACGGCAAGTGCAGCGTGCCTTGATGCACAAAATGCTGACTCCGATGCACCGAAACGCGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGaa                                                                                                                 >  4:109316/1‑147 (MQ=255)                        tAGCAATTCCTGGGCCTATTATTGGCAAAGCCTTGTACGACGGCAAGTGCAGCGTGCCTTGATGCACAAAATGCTGACTCCGATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAg                                                                                                              >  1:257637/1‑149 (MQ=255)                         aGCAATTCCTGGGCCTATTATTGGCAAAGCCTTGTACGACGGCAAGTGCAGCGTGCCTTGATGCACAAAATGCTGACTCCGATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGaa                                                                                                                 <  6:6130/145‑1 (MQ=255)                         aGCAATTCCTGGGCCTATTATTGGCAAAGCCTTGTACGACGGCAAGTGCAGCGTGCCTTGATGCACAAAATGCTGACTCCGATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGaa                                                                                                                 >  5:6130/1‑145 (MQ=255)                            aaTTCCTGGGCCTATTATTGGCAAAGCCTTGTACGACGGCAAGTGCAGCGTGCCTTGATGCACAAAATGCTGATTCCGATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAAc                                                                                                          <  8:39658/149‑1 (MQ=255)                                ccTGGGCCTATTATTGGCAAAGCCTTGTACGACGGCAAGTGCAGCGTGCCTTGATGCACAAAATGCTGACTCCGATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCga                                                                                                       <  8:202930/148‑1 (MQ=255)                                    ggCCTATTATTGGCAAAGCCTTGTACGACGGCAAGTGCAGCGTGCCTTGATGCACAAAATGCTGACTCCGATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACa                                                                                                               >  1:15195/1‑136 (MQ=255)                                    ggCCTATTATTGGCAAAGCCTTGTACGACGGCAAGTGCAGCGTGCCTTGATGCACAAAATGCTGACTCCGATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACa                                                                                                               <  2:15195/136‑1 (MQ=255)                                    ggCCTATTATTGGCAAAGCCTTGTACGACGGCAAGTGCAGCGTGCCTTGATGCACAAAATGCTGACTCCGATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCgatgat                                                                                                   <  2:257637/148‑1 (MQ=255)                                    ggCCTATTATTGGCAAAGCCTTGTACGACGGCAAGTGCAGCGTGCCTTGATGCACAAAATGCTGACTCCGATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGAtt                                                                                                  <  1:256044/149‑1 (MQ=255)                                      ccTATTATTGGCAAAGCCTTGTACGACGGCAAGTGCAGCGTGCCTTGATGCACAAAATGCTGACTCCGATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGaa                                                                                                                 <  3:302308/132‑1 (MQ=255)                                      ccTATTATTGGCAAAGCCTTGTACGACGGCAAGTGCAGCGTGCCTTGATGCACAAAATGCTGACTCCGATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGaa                                                                                                                 >  4:302308/1‑132 (MQ=255)                                      ccTATTATTGGCAAAGCCTTGTACGACGGCAAGTGCAGCGTGCCTTGATGCACAAAATGCTGACTCCGATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGt                                                                                                >  1:336383/1‑149 (MQ=255)                                        tattatTGGCAAAGCCTTGTACGACGGCAAGTGCAGCGTGCCTTGATGCACAAAATGCTGACTCCGATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCg                                                                                              >  7:264492/1‑149 (MQ=255)                                         attattGGCAAAGCCTTGTACGACGGCAAGTGCAGCGTGCCTTGATGCACAAAATGCTGACTCCGATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCgg                                                                                             <  7:64960/149‑1 (MQ=255)                                          ttattGGCAAAGCCTTGTACGACGGCAAGTGCAGCGTGCCTTGATGCACAAAATGCTGACTCCGATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCggg                                                                                            >  4:137617/1‑149 (MQ=255)                                            attGGCAAAGCCTTGTACGACGGCAAGTGCAGCGTGCCTTCATGCACAAAATGCTGACTCCGATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGt                                                                                           <  5:317761/148‑1 (MQ=255)                                                gCAAAGCCTTGTACGACGGCAAGTGCAGCGTGCCTTGATGCACAAAATGCTGACTCCGATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGCACAGGTTCGGGGcgcca                                                                                                                        <  5:230402/115‑5 (MQ=255)                                                gCAAAGCCTTGTACGACGGCAAGTGCAGCGTGCCTTGATGCACAAAATGCTGACTCCGATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGCACAGGTTCGGGGcgcca                                                                                                                        >  6:230402/1‑111 (MQ=255)                                                  aaaGCCTTGTACGACGGCAAGTGCAGCGTGCCTTGATGCACAAATTGCTGACTCCGATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATg                                                                                    >  1:77708/1‑149 (MQ=255)                                                      ccTTGTACGACGGCAAGTGCAGCGTGCCTTGATGCACAAAATGCTGACTCCGATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCaa                                                                                 >  8:192691/1‑148 (MQ=255)                                                              gacgGCAAGTGCAGCGTGCCTTGATGCACAAAATGCTGACTCCGATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCg                                                                                                                          >  8:62016/1‑99 (MQ=255)                                                              gacgGCAAGTGCAGCGTGCCTTGATGCACAAAATGCTGACTCCGATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCg                                                                                                                          <  7:62016/99‑1 (MQ=255)                                                              gacgGCAAGTGCAGCGTGCCTTGATGCACAAAATGCTGACTCCGATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCt                                                                        >  6:134347/1‑149 (MQ=255)                                                                   cAAGTGCAGCGTGCCTTGATGCACAAAATGCTGACTCCGATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGccc                                                                   >  1:80996/1‑149 (MQ=255)                                                                     aGTGCAGCGTGCCTTGATGCACAAAATGCTGACTCCGATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCcgc                                                                 <  1:185192/149‑1 (MQ=255)                                                                        gCAGCGTGCCTTGATGCACAAAATGCTGACTCCGATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGaa                                                              >  2:311844/1‑149 (MQ=255)                                                                          aGCGTGCCTTGATGCACAAAATGCTGACTCCGATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAAc                                                                                                          <  4:218828/103‑1 (MQ=255)                                                                          aGCGTGCCTTGATGCACAAAATGCTGACTCCGATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAAc                                                                                                          >  3:218828/1‑103 (MQ=255)                                                                           gCGTGCCTTGATGCACAAAATGCTGACTCCGATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGc                                                           <  8:81761/149‑1 (MQ=255)                                                                             gTGCCTTGATGCACAAAATGCTGACTCCGATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGt                                                         <  6:43416/149‑1 (MQ=255)                                                                                ccTTGATGCACAAAATGCTGACTCCGATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAg                                                      <  8:264492/149‑1 (MQ=255)                                                                                 cTTGATGCACAAAATGCTGACTCCGATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATg                                                                                    <  1:83073/118‑1 (MQ=255)                                                                                 cTTGATGCACAAAATGCTGACTCCGATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATg                                                                                    >  2:83073/1‑118 (MQ=255)                                                                                      tGCACAAAATGCTGACTCCGATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAgg                                                <  3:109316/149‑1 (MQ=255)                                                                                         acaAAATGCTGACTCCGATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGa                                             <  2:271582/149‑1 (MQ=255)                                                                                             aaTGCTGACTCCGATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGc                                         <  5:134347/149‑1 (MQ=255)                                                                                                    aCTCCGATGCACCTAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGc                                   <  1:311844/147‑1 (MQ=255)                                                                                                      tCCGATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTg                                >  4:170705/1‑148 (MQ=255)                                                                                                           tGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGa                          <  2:77708/149‑1 (MQ=255)                                                                                                                  aaaCGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGGGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTc                   >  5:341837/1‑149 (MQ=255)                                                                                                                    aCGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTcgc                 <  2:80996/149‑1 (MQ=255)                                                                                                                    aCGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTcgc                 <  3:170705/149‑1 (MQ=255)                                                                                                                       ggCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCggg              <  3:137617/149‑1 (MQ=255)                                                                                                                       ggCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTCAGTTCAGGCTA‑AGCTGTCCCCTGACCTCACGCATTCGCggg              <  4:331544/149‑1 (MQ=21)                                                                                                                         ccGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAGAGCGGGCTTGCCCGCGAATGCGTCAGTTCAGGCTA‑AGCTGTCCCCTGACCTCACGCATTcgc                 <  2:165733/144‑1 (MQ=18)                                                                                                                         ccGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAGAGCGGGCTTGCCCGCGAATGCGTCAGTTCAGGCTA‑AGCTGTCCCCTGACCTCACGCATTcgc                 >  1:165733/1‑144 (MQ=18)                                                                                                                            gAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCTGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGcttgc         >  1:47910/1‑145 (MQ=255)                                                                                                                                    tCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCTGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCTTGCCCGcgaa  <  2:47910/148‑4 (MQ=255)                                                                                                                                                 |                                                                                                                                        GCAAGAAATAGAACCTATAGACCTAGCAATTCCTGGGCCTATTATTGGCAAAGCCTTGTACGACGGCAAGTGCAGCGTGCCTTGATGCACAAAATGCTGACTCCGATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAAGCCCGCTCC  >  NC_002947/5096997‑5097275

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑