Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,097,148 G→T 6.2% intergenic (+139/+153) PP_mr66 → / ← acsA‑I P6/acetyl‑CoA synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,097,1480GT 6.2% 206.1 / 3.5 64intergenic (+139/+153)PP_mr66/acsA‑IP6/acetyl‑CoA synthetase
Reads supporting (aligned to +/- strand):  ref base G (25/35);  new base T (2/2);  total (27/37)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.75e-01

GAAATAGAACCTATAGACCTAGCAATTCCTGGGCCTATTATTGGCAAAGCCTTGTACGACGGCAAGTGCAGCGTGCCTTGATGCACAAAATGCTGACTCCGATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAAGCCCGCTCC  >  NC_002947/5097001‑5097275
                                                                                                                                                   |                                                                                                                                
gAAATAGAACCTATAGACCTAGCAATTCCTGGGCCTATTATTGGCAAAGCCTTGTACGACGGCAAGTGCAGCGTGCCTTGATGCACAAAATGCTGACTCCGATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGc                                                                                                                                 >  2:256044/1‑149 (MQ=255)
  aaTAGAACCTATAGACCTAGCAATTCCTGGGCCTATTATTGGCAAAGCCTTGTACGACGGCAAGTGCAGCGTGCCTTGATGCACAAAATGCTGACTCCGATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCgg                                                                                                                               >  2:185192/1‑149 (MQ=255)
   aTAGAACCTATAGACCTAGCAATTCCTGGGCCTATTATTGGCAAAGCCTTGTACGACGGCAAGTGCAGCGTGCCTTGATGCACAAAATGCTGACTCCGATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCggg                                                                                                                              <  8:272370/149‑1 (MQ=255)
      gAACCTATAGACCTAGCAATTCCTGGGCCTATTATTGGCAAAGCCTTGTACGACGGCAAGTGCAGCGTGCCTTGATGCACAAAATGCTGACTCCGATGCACCGAAACGGGGCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGcc                                                                                                                           >  7:284216/1‑149 (MQ=255)
         ccTATAGACCTAGCAATTCCTGGGCCTATTATTGGCAAAGCCTTGTACGACGGCAAGTGCAGCGTGCCTTGATGCACAAAATGCTGACTCCGATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGt                                                                                                                        <  1:236537/149‑1 (MQ=255)
         ccTATAGACCTAGCAATTCCTGGGCCTATTATTGGCAAAGCCTTGTACGACGGCAAGTGCAGCGTGCCTTGATGCACAAAATGCTGACTCCGATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGt                                                                                                                        <  6:110738/149‑1 (MQ=255)
          cTATAGACCTAGCAATTCCTGGGCCTATTATTGGCAAAGCCTTGTACGACGGCAAGTGCAGCGTGCCTTGATGCACAAAATGCTGACTCCGATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGCACAGGTTCgggg                                                                                                                             <  1:207646/143‑1 (MQ=255)
          cTATAGACCTAGCAATTCCTGGGCCTATTATTGGCAAAGCCTTGTACGACGGCAAGTGCAGCGTGCCTTGATGCACAAAATGCTGACTCCGATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGCACAGGTTCgggg                                                                                                                             >  2:207646/1‑143 (MQ=255)
             taGACCTAGCAATTCCTGGGCCTATTATTGTCAAAGCCTTGTACGACGGCAAGTGCAGCGTGCCTTGATGCACAAAATGCTGACTCCGATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGa                                                                                                                      <  8:22525/147‑1 (MQ=255)
             taGACCTAGCAATTCCTGGGCCTATTATTGGCAAAGCCTTGTACGACGGCAAGTGCAGCGTGCCTTGATGCACAAAATGCTGACTCCGATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACa                                                                                                                    <  5:134651/149‑1 (MQ=255)
             taGACCTAGCAATTCCTGGGCCTATTATTAGCAAAGCCTTGTACGACGGCAAGTGCAGCGTGCCTTGATGCACAAAATGCTGACTCCGATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGa                                                                                                                      >  6:45711/1‑147 (MQ=255)
             taGACCTAGCAATTCCTGGGCCTATTATTAGCAAAGCCTTGTACGACGGCAAGGGCAGCGTGCCTTGATGCACAAAATGCTGACTCCGATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGa                                                                                                                      <  5:45711/147‑1 (MQ=255)
               gACCTAGCAATTCCTGGGCCTATTATTGGCAAAGCCTTGTACGACGGCAAGTGCAGCGTGCCTTGATGCACAAAATGCTGACTCCGATGCACCGAAACGGGGCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAg                                                                                                                   <  8:284216/148‑1 (MQ=255)
               gACCTAGCAATTCCTGGGCCTATTATTGGCAAAGCCTTGTACGACGGCAAGTGCAGCGTGCCTTGATGCACAAAATGCTGACTCCGATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAg                                                                                                                   <  4:230/148‑1 (MQ=255)
                  cTAGCAATTCCTGGGCCTATTATTGGCAAAGCCTTGTACGACGGCAAGTGCAGCGTGCCTTGATGCACAAAATGCTGATTCCGATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGaa                                                                                                                 >  7:39658/1‑147 (MQ=255)
                  cTAGCAATTCCTGGGCCTATTATTGGCAAAGCCTTGTACGACGGCAAGTGCAGCGTGCCTTGATGCACAAAATGCTGACTCCGATGCACCGAAACGCGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGaa                                                                                                                 >  4:109316/1‑147 (MQ=255)
                   tAGCAATTCCTGGGCCTATTATTGGCAAAGCCTTGTACGACGGCAAGTGCAGCGTGCCTTGATGCACAAAATGCTGACTCCGATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAg                                                                                                              >  1:257637/1‑149 (MQ=255)
                    aGCAATTCCTGGGCCTATTATTGGCAAAGCCTTGTACGACGGCAAGTGCAGCGTGCCTTGATGCACAAAATGCTGACTCCGATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGaa                                                                                                                 <  6:6130/145‑1 (MQ=255)
                    aGCAATTCCTGGGCCTATTATTGGCAAAGCCTTGTACGACGGCAAGTGCAGCGTGCCTTGATGCACAAAATGCTGACTCCGATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGaa                                                                                                                 >  5:6130/1‑145 (MQ=255)
                       aaTTCCTGGGCCTATTATTGGCAAAGCCTTGTACGACGGCAAGTGCAGCGTGCCTTGATGCACAAAATGCTGATTCCGATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAAc                                                                                                          <  8:39658/149‑1 (MQ=255)
                           ccTGGGCCTATTATTGGCAAAGCCTTGTACGACGGCAAGTGCAGCGTGCCTTGATGCACAAAATGCTGACTCCGATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCga                                                                                                       <  8:202930/148‑1 (MQ=255)
                               ggCCTATTATTGGCAAAGCCTTGTACGACGGCAAGTGCAGCGTGCCTTGATGCACAAAATGCTGACTCCGATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACa                                                                                                               <  2:15195/136‑1 (MQ=255)
                               ggCCTATTATTGGCAAAGCCTTGTACGACGGCAAGTGCAGCGTGCCTTGATGCACAAAATGCTGACTCCGATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACa                                                                                                               >  1:15195/1‑136 (MQ=255)
                               ggCCTATTATTGGCAAAGCCTTGTACGACGGCAAGTGCAGCGTGCCTTGATGCACAAAATGCTGACTCCGATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCgatgat                                                                                                   <  2:257637/148‑1 (MQ=255)
                               ggCCTATTATTGGCAAAGCCTTGTACGACGGCAAGTGCAGCGTGCCTTGATGCACAAAATGCTGACTCCGATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGAtt                                                                                                  <  1:256044/149‑1 (MQ=255)
                                 ccTATTATTGGCAAAGCCTTGTACGACGGCAAGTGCAGCGTGCCTTGATGCACAAAATGCTGACTCCGATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGaa                                                                                                                 <  3:302308/132‑1 (MQ=255)
                                 ccTATTATTGGCAAAGCCTTGTACGACGGCAAGTGCAGCGTGCCTTGATGCACAAAATGCTGACTCCGATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGaa                                                                                                                 >  4:302308/1‑132 (MQ=255)
                                 ccTATTATTGGCAAAGCCTTGTACGACGGCAAGTGCAGCGTGCCTTGATGCACAAAATGCTGACTCCGATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGt                                                                                                >  1:336383/1‑149 (MQ=255)
                                   tattatTGGCAAAGCCTTGTACGACGGCAAGTGCAGCGTGCCTTGATGCACAAAATGCTGACTCCGATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCg                                                                                              >  7:264492/1‑149 (MQ=255)
                                    attattGGCAAAGCCTTGTACGACGGCAAGTGCAGCGTGCCTTGATGCACAAAATGCTGACTCCGATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCgg                                                                                             <  7:64960/149‑1 (MQ=255)
                                     ttattGGCAAAGCCTTGTACGACGGCAAGTGCAGCGTGCCTTGATGCACAAAATGCTGACTCCGATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCggg                                                                                            >  4:137617/1‑149 (MQ=255)
                                       attGGCAAAGCCTTGTACGACGGCAAGTGCAGCGTGCCTTCATGCACAAAATGCTGACTCCGATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGt                                                                                           <  5:317761/148‑1 (MQ=255)
                                           gCAAAGCCTTGTACGACGGCAAGTGCAGCGTGCCTTGATGCACAAAATGCTGACTCCGATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGCACAGGTTCGGGGcgcca                                                                                                                        >  6:230402/1‑111 (MQ=255)
                                           gCAAAGCCTTGTACGACGGCAAGTGCAGCGTGCCTTGATGCACAAAATGCTGACTCCGATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGCACAGGTTCGGGGcgcca                                                                                                                        <  5:230402/115‑5 (MQ=255)
                                             aaaGCCTTGTACGACGGCAAGTGCAGCGTGCCTTGATGCACAAATTGCTGACTCCGATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATg                                                                                    >  1:77708/1‑149 (MQ=255)
                                                 ccTTGTACGACGGCAAGTGCAGCGTGCCTTGATGCACAAAATGCTGACTCCGATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCaa                                                                                 >  8:192691/1‑148 (MQ=255)
                                                         gacgGCAAGTGCAGCGTGCCTTGATGCACAAAATGCTGACTCCGATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCg                                                                                                                          <  7:62016/99‑1 (MQ=255)
                                                         gacgGCAAGTGCAGCGTGCCTTGATGCACAAAATGCTGACTCCGATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCg                                                                                                                          >  8:62016/1‑99 (MQ=255)
                                                         gacgGCAAGTGCAGCGTGCCTTGATGCACAAAATGCTGACTCCGATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCt                                                                        >  6:134347/1‑149 (MQ=255)
                                                              cAAGTGCAGCGTGCCTTGATGCACAAAATGCTGACTCCGATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGccc                                                                   >  1:80996/1‑149 (MQ=255)
                                                                aGTGCAGCGTGCCTTGATGCACAAAATGCTGACTCCGATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCcgc                                                                 <  1:185192/149‑1 (MQ=255)
                                                                   gCAGCGTGCCTTGATGCACAAAATGCTGACTCCGATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGaa                                                              >  2:311844/1‑149 (MQ=255)
                                                                     aGCGTGCCTTGATGCACAAAATGCTGACTCCGATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAAc                                                                                                          <  4:218828/103‑1 (MQ=255)
                                                                     aGCGTGCCTTGATGCACAAAATGCTGACTCCGATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAAc                                                                                                          >  3:218828/1‑103 (MQ=255)
                                                                      gCGTGCCTTGATGCACAAAATGCTGACTCCGATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGc                                                           <  8:81761/149‑1 (MQ=255)
                                                                        gTGCCTTGATGCACAAAATGCTGACTCCGATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGt                                                         <  6:43416/149‑1 (MQ=255)
                                                                           ccTTGATGCACAAAATGCTGACTCCGATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAg                                                      <  8:264492/149‑1 (MQ=255)
                                                                            cTTGATGCACAAAATGCTGACTCCGATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATg                                                                                    >  2:83073/1‑118 (MQ=255)
                                                                            cTTGATGCACAAAATGCTGACTCCGATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATg                                                                                    <  1:83073/118‑1 (MQ=255)
                                                                                 tGCACAAAATGCTGACTCCGATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAgg                                                <  3:109316/149‑1 (MQ=255)
                                                                                    acaAAATGCTGACTCCGATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGa                                             <  2:271582/149‑1 (MQ=255)
                                                                                        aaTGCTGACTCCGATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGc                                         <  5:134347/149‑1 (MQ=255)
                                                                                               aCTCCGATGCACCTAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGc                                   <  1:311844/147‑1 (MQ=255)
                                                                                                 tCCGATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTg                                >  4:170705/1‑148 (MQ=255)
                                                                                                      tGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGa                          <  2:77708/149‑1 (MQ=255)
                                                                                                             aaaCGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGGGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTc                   >  5:341837/1‑149 (MQ=255)
                                                                                                               aCGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTcgc                 <  3:170705/149‑1 (MQ=255)
                                                                                                               aCGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTcgc                 <  2:80996/149‑1 (MQ=255)
                                                                                                                  ggCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCggg              <  3:137617/149‑1 (MQ=255)
                                                                                                                  ggCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTCAGTTCAGGCTA‑AGCTGTCCCCTGACCTCACGCATTCGCggg              <  4:331544/149‑1 (MQ=21)
                                                                                                                    ccGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAGAGCGGGCTTGCCCGCGAATGCGTCAGTTCAGGCTA‑AGCTGTCCCCTGACCTCACGCATTcgc                 <  2:165733/144‑1 (MQ=18)
                                                                                                                    ccGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAGAGCGGGCTTGCCCGCGAATGCGTCAGTTCAGGCTA‑AGCTGTCCCCTGACCTCACGCATTcgc                 >  1:165733/1‑144 (MQ=18)
                                                                                                                       gAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCTGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGcttgc         >  1:47910/1‑145 (MQ=255)
                                                                                                                               tCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCTGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCTTGCCCGcgaa  <  2:47910/148‑4 (MQ=255)
                                                                                                                                                   |                                                                                                                                
GAAATAGAACCTATAGACCTAGCAATTCCTGGGCCTATTATTGGCAAAGCCTTGTACGACGGCAAGTGCAGCGTGCCTTGATGCACAAAATGCTGACTCCGATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAAGCCCGCTCC  >  NC_002947/5097001‑5097275

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑