Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,097,432	T→A	6.4%	M654L (ATG→TTG)	acsA‑I ←	acetyl‑CoA synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,097,432	0	T	A	6.4%	227.1 / 3.3	73	M654L (ATG→TTG)	acsA‑I	acetyl‑CoA synthetase
Reads supporting (aligned to +/- strand):  ref base T (36/32);  new base A (3/2);  total (39/34)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCGGGTCAGCCAAGGTCGAGATATCGCCCAGGGCGTCGTACTCACCGGTGGCGATCTTGCGCAGGATGCGGCGCATGATCTTGCCCGAGCGGGTCTTCGGCAGCCCCGGCGCCCACTGGATCACATCCGGCGAAGCAATCGG‑GCCGATCTCCTTGCGCACCCAGTTCTTCAGCTCCAGGCGCAATTGCTCGCTGGCCTCGATGCCGGCATTGAGGGTGACA  >  NC_002947/5097299‑5097577                                                                                                                                      |                                                                                                                                                   gTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCGGGTCAGCCAAGGTCGAGATATCGCCCAGGGCGTCGTACTCACCGGTGGCGATCTTGCGCAGGATGCGGCGCATGATCTTGCCCGAGCg                                                                                                                                     >  8:31103/1‑149 (MQ=255)  tttACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCGGGTCAGCCAAGGTCGAGATATCGCCCAGGGCGTCGTACTCACCGGTGGCGATCTTGCGCAGGATGCGGCGCATGATCTTGCCCGAGCgg                                                                                                                                    <  5:203765/149‑1 (MQ=255)       gCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTATCACACCCGGGTCAGCCAAGGTCGAGATATCGCCCAGGGCGTCGTACTCACCGGTGGCGATCTTGCGCAGGATGCGGCGCATGATCTTGCCCGAGCGGGTCtt                                                                                                                               >  8:36020/1‑149 (MQ=255)        ccGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCGGGTCAGCCAAGGTCGAGATATCGCCCAGGGCGTCGTACTCACCGGTGGCGATCTTGCGCAGGATGCGGCGCATGATCTTGCCCGAGCGGGTCTTc                                                                                                                              <  4:176878/149‑1 (MQ=255)        ccGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCGGGTCAGCCAAGGTCGAGATATCGCCCAGGGCGTCGTACTCACCGGTGGCGATCTTGCGCAGGATGCGGCGCATGATCTTGCCCGAGCGGGTCTTc                                                                                                                              <  5:211989/149‑1 (MQ=255)            aGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCGGGTCAGCCAAGGTCGAGATATCGCCCAGGGCGTCGTACTCACCGGTGGCGATCTTGCGCAGGATGCGGCGCATGATCTTGCCCGAGCGGGTCTTCgg                                                                                                                            >  4:269627/1‑147 (MQ=255)                  gTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCGGGTCAGCCAAGGTCGAGATATCGCCCAGGGCGTCGTACTCACCGGTGGCGATCTTGCGCAGGATGCGGCGCATGATCTTGCCCGAGCGGGTCTTCGGCAGCCCCg                                                                                                                    <  2:191553/149‑1 (MQ=255)                              gtgGGTATCGATCAGGTGCTGTACCACACCCGGGTCAGCCAAGGTCGAGATATCGCCCAGGGCGTCGTACTCACCGGTGGCGATCTTGCGCAGGATGCGGCGCATGATCTTGCCCGAGCGGGTCTTCGGCAGCCCCGGCGCCCACTgg                                                                                                         <  4:49537/148‑1 (MQ=255)                               tgGGTATCGATCAGGTGCTGTACCACACCCGGGTCAGCCAAGGTCGAGATATCGCCCAGGGCGTCGTACTCACCGGTGGCGATCTTGCGCAGGATGCGGCGCATGATCTTGCCCGa                                                                                                                                        <  1:356920/116‑1 (MQ=255)                               tgGGTATCGATCAGGTGCTGTACCACACCCGGGTCAGCCAAGGTCGAGATATCGCCCAGGGCGTCGTACTCACCGGTGGCGATCTTGCGCAGGATGCGGCGCATGATCTTGCCCGa                                                                                                                                        >  2:356920/1‑116 (MQ=255)                               tgGGTATCGATCAGGTGCTGTACCACACCCGGGTCAGCCAAGGTCGAGATATCGCCCAGGGCGTCGTACTCACCGGTGGCGATCTTGCGCAGGATGCGGCGCATGATCTTGCCCGAGCGGGTCTTCGGCAGCCCCGGCGCCCACTGGAt                                                                                                       <  3:345992/149‑1 (MQ=255)                                 ggTATCGATCAGGTGCTGTACCACACCCGGGTCAGCCAAGGTCGAGATATCGCCCAGGGCGTCGTACTCACCGGTGGCGATCTTGCGCAGGATGCGGCGCATGATCTTGCCCGAGCGGGTCTTCGGCAGCCCCGGCGCCCACTGGATc                                                                                                      <  5:125412/148‑1 (MQ=255)                                       gATCAGGTGCTGTACCACACCCGGGTCAGCCAAGGTCGAGATATCGCCCAGGGCGTCGTACTCACCGGTGGCGATCTTGCGCAGGATGCGGCGCATGATCTTGCCCGAGCGGGTCTTCGGCAGCCCCGGCGCCCACTGGATCACATCCg                                                                                               >  5:51098/1‑149 (MQ=255)                                                 tGTACCACACCCGGGTCAGCCAAGGTCGAGATATCGCCCAGGGCGTCGTACTCACCGGTGGCGATCTTGCGCAGGATGCGGCGCATGATCTTGCCCGAGCGGGTCTTCGGCAGCCCCGGCGCCCACTg                                                                                                          <  8:90747/128‑1 (MQ=255)                                                 tGTACCACACCCGGGTCAGCCAAGGTCGAGATATCGCCCAGGGCGTCGTACTCACCGGTGGCGATCTTGCGCAGGATGCGGCGCATGATCTTGCCCGAGCGGGTCTTCGGCAGCCCCGGCGCCCACTg                                                                                                          >  7:90747/1‑128 (MQ=255)                                                   tACCACACCCGGGTCAGCCAAGGTCGAGATATCGCCCAGGGCGTCGTACTCACCGGTGGCGATCTTGCGCAGGATGCGGCGCATGATCTTGCCCGAGCGGGTCTTCGGCAGCCCCGGCGCCCACTGGATCACATCCGGCGAAGCAATCg                                                                                   <  7:31103/149‑1 (MQ=255)                                                     ccACACCCGGGTCAGCCAAGGTCGAGATATCGCCCAGGGCGTCGTACTCACCGGTGGCGATCTTGCGCAGGATGCGGCGCATGATCTTGCCCGAGCGGGTCTTCGGCAGCCCCGGCGcc                                                                                                               <  6:313970/119‑1 (MQ=255)                                                     ccACACCCGGGTCAGCCAAGGTCGAGATATCGCCCAGGGCGTCGTACTCACCGGTGGCGATCTTGCGCAGGATGCGGCGCATGATCTTGCCCGAGCGGGTCTTCGGCAGCCCCGGCGcc                                                                                                               >  5:313970/1‑119 (MQ=255)                                                      cacaCCCGGGTCAGCCAAGGTCGAGATATCGCCCAGGGCGTCGTACTCACCGGTGGCGATCTTGCGCAGGATGCGGCGCATGATCTTGCCCGAGCgg                                                                                                                                    <  3:282151/97‑1 (MQ=255)                                                      cacaCCCGGGTCAGCCAAGGTCGAGATATCGCCCAGGGCGTCGTACTCACCGGTGGCGATCTTGCGCAGGATGCGGCGCATGATCTTGCCCGAGCgg                                                                                                                                    >  4:282151/1‑97 (MQ=255)                                                         acCCGGGTCAGCCAAGGTCGAGATATCGCCCAGGGCGTCGTACTCACCGGTGGCGATCTTGCGCAGGATGCGGCGCATGATCTTGCCCGAGCGGGTCTTCGGCAGCCCCGGCGCCCACTGGATCACATCCGGCGAAGCAATCGG‑GCCGa                                                                            >  4:136048/1‑149 (MQ=255)                                                             gggTCAGCCAAGGTCGAGATATCGCCCAGGGCGTCGTACTCACCGGTGGCGATCTTGCGCAGGATGCGGCGCATGATCTTGCCCGAGCGGGTCTTCGGCAGCCCCGGCGCCCACTGGATCACATCCGGCGAAGCAATCGG‑GCCGAtctc                                                                        >  5:324886/1‑149 (MQ=255)                                                             gggTCAGCCAAGGTCGAGATATCGCCCAGGGCGTCGTACTCACCGGTGGCGATCTTGCGCAGGATGCGGCGCATGATCTTGCCCGAGCGGGTCTTCGGCAGCCCCGGCGCCCACTGGATCACATCCGGCGAAGCAATCGG‑GCCGAtctc                                                                        >  2:339807/1‑149 (MQ=255)                                                                tCAGCCAAGGTCGAGATATCGCCCAGGGCGTCGTACTCACCGGTGGCGATCTTGCGCAGGATGCGGCGCATGATCTTGCCCGAGCGGGTCTTCGGCAGCCCCGGCGCCCACTGGATCACATCCGGCGAAGCAATCGG‑GCCGATCTCCtt                                                                     <  6:324886/149‑1 (MQ=255)                                                                tCAGCCAAGGTCGAGATATCGCCCAGGGCGTCGTACTCACCGGTGGCGATCTTGCGCAGGATGCGGCGCATGATCTTGCCCGAGCGGGTCTTCGGCAGCCCCGGCGCCCACTGGATCACATCCGGCGAAGCAATCGG‑GCCGATCTCCtt                                                                     <  1:275503/149‑1 (MQ=255)                                                                  aGCCAAGGTCGAGATATCGCCCAGGGCGTCGTACTCACCGGTGGCGATCTTGCGCAGGATGCGGCGCATGATCTTGCCCGAGCGGGTCTTCGGCAGCCCCGGCGCCCACTGGATCACATCCGGCGAAGCAATCGG‑GCCGATCTCCGTgc                                                                   >  4:40903/1‑149 (MQ=255)                                                                    ccAAGGTCGAGATATCGCCCAGGGCGTCGTACTCACCGGTGGCGATCTTGCGCAGGATGCGGCGCATGATCTTGCCCGAGCGGGTCTTCGGCAGCCCCGGCGCCCACTGGATCACATCCGGCGAAGCAATCGG‑GCCGATCTCCTTgcg                                                                  >  2:177854/1‑148 (MQ=255)                                                                       aGGTCGAGATATCGCCCAGGGCGTCGTACTCACCGGTGGCGATCTTGCGCAGGATGCGGCGCATGATCTTGCCCGAGCGGGTCTTCGGCAGCCCCGGCGCCCACTGGATCACATCCGGCGAAGCAATCGG‑GCCGATCTCCTTGCGCAcc                                                              <  2:316082/149‑1 (MQ=255)                                                                           cGAGATATCGCCCAGGGCGTCGTACTCACCGGTGGCGATCTTGCGCAGGATGCGGCGCATGATCTTGCCCGAGCGGGTCTTCGGCAGCCCCGGCGCCCACTGGATCACATCCGGCGAAGCAATCGG‑GCCGATCTCCTTGCGCACCCAGt                                                          >  4:23595/1‑149 (MQ=255)                                                                            gagaTATCGCCCAGGGCGTCGTACTCACCGGTGGCGATCTTGCGCAGGATGCGGCGCATGATCTTGCCCGAGCGGGTCTTCGGCAGCCCCGGCGCCCACTGGATCACATCCGGCGAAGCAATCGG‑Gc                                                                               <  4:318611/127‑1 (MQ=255)                                                                            gagaTATCGCCCAGGGCGTCGTACTCACCGGTGGCGATCTTGCGCAGGATGCGGCGCATGATCTTGCCCGAGCGGGTCTTCGGCAGCCCCGGCGCCCACTGGATCACATCCGGCGAAGCAATCGG‑Gc                                                                               >  3:318611/1‑127 (MQ=255)                                                                            gagaTATCGCCCAGGGCGTCGTACTCACCGGTGGCGATCTTGCGCAGGATGCGGCGCATGATCTTGCCCGAGCGGGTCTTCGGCAGCCCCGGCGCCCACTGGATCACATCCGGCGAAGCAATCGG‑GCCGATCTCCtt                                                                     >  4:135049/1‑137 (MQ=255)                                                                            gagaTATCGCCCAGGGCGTCGTACTCACCGGTGGCGATCTTGCGCAGGATGCGGCGCATGATCTTGCCCGAGCGGGTCTTCGGCAGCCCCGGCGCCCACTGGATCACATCCGGCGAAGCAATCGG‑GCCGATCTCCtt                                                                     <  3:135049/137‑1 (MQ=255)                                                                            gagaTATCGCCCAGGGCGTCGTACTCACCGGTGGCGATCTTGCGCAGGATGCGGCGCATGATCTTGCCCGAGCGGGTCTTCGGCAGCCCCGGCGCCCACTGGATCACATCCGGCGAAGCAATCGG‑GCCGATCTCCTTGCGCACCCAGt                                                          >  3:352817/1‑148 (MQ=255)                                                                              gaTATCGCCCAGGGCGTCGTACTCACCGGTGGCGATCTTGCGCAGGATGCGGCGCATGATCTTGCCCGAGCGGGTCTTCGGCAGCCCCGGCGCCCACTGGATCACATCCGGCGAAGCAATCGG‑GCCGATCTCCTTGCGCACCCAGttct                                                       >  2:220561/1‑149 (MQ=255)                                                                                tatCGCCCAGGGCGTCGTACTCACCGGTGGCGATCTTGCGCAGGATGCGGCGCATGATCTTGCCCGAGc                                                                                                                                      >  1:29496/1‑69 (MQ=255)                                                                                tatCGCCCAGGGCGTCGTACTCACCGGTGGCGATCTTGCGCAGGATGCGGCGCATGATCTTGCCCGAGc                                                                                                                                      <  2:29496/69‑1 (MQ=255)                                                                                     cccAGGGCGTCGTACTCACCGGTGGCGATCTTGCGCAGGATGCGGCGCATGATCTTGCCCGAGCGGGTCTTCGGCAGCCCCGGCGCCCACTGGATCACATCCGGCGAAGCAATCGG‑GCCGATCTCCTTGCGCACCCAGTTCTTCAGCTc                                                >  8:183574/1‑149 (MQ=255)                                                                                      ccAGGGCGTCGTACTCACCGGTGGCGATCTTGCGCAGGATGCGGCGCATGATCTTGCCCGAGCGGGTCTTCGGCAGCCCCGGCGCCCACTGGATCACATCCGGCGAAGCAATCGG‑GCCGATCTCCTTGCGCACCCAGTTCTTCAGCTc                                                >  2:49639/1‑148 (MQ=255)                                                                                       cAGGGCGTCGTACTCACCGGTGGCGATCTTGCGCAGGATGCGGCGCATGATCTTGCCCGAGCGGGTCTTCGGCAGCCCCGGCGCCCACTGGATCACATCCGGCGAAGCAATCGGCGCCGATCTCCTTGCGCACCCAGTTCTTCAGCTcc                                               <  1:49639/149‑1 (MQ=255)                                                                                          ggCGTCGTACTCACCGGTGGCGATCTTGCGCAGGATGCGGCGCATGATCTTGCCCGAGCGGGTCTTCGGCAGCCCCGGCGCCCACTGGATCACATCCGGCGAAGCAATCGG‑GCCGATCTCCTTGCGCACCCAGTTCTTCAGCTCCAGgc                                           >  4:211917/1‑149 (MQ=255)                                                                                          ggCGTCGTACTCACCGGTGGCGATCTTGCGCAGGATGCGGCGCATGATCTTGCCCGAGCGGGTCTTCGGCAGCCCCGGCGCCCACTGGATCACATCCGGCGAAGCAATCGG‑GCCGATCTCCTTGCGCACCCAGTTCTTCAGCTCCAGgc                                           <  3:40903/149‑1 (MQ=255)                                                                                                  aCTCACCGGTGGCGATCTTGCGCAGGATGCGGCGCATGATCTTGCCCGAGCGGGTCTTCGGCAGCCCCGGCGCCCACTGGATCACATCCGGCGAAGCAATCGG‑GCCGATCTCCTTGCGCACCCAGTTCTTCAGCTCCAGGCGCAATTGc                                   >  6:251124/1‑149 (MQ=255)                                                                                                  aCTCACCGGTGGCGATCTTGCGCAGGATGCGGCGCATGATCTTGCCCGAGCGGGTCTTCGGCAGCCCCGGCGCCCACTGGATCACATCCGGCGAAGCAATCGG‑GCCGATCTCCTTGCGCACCCAGTTCTTCAGCTCCAGGCGCAATTGc                                   >  5:63419/1‑149 (MQ=255)                                                                                                    tCACCGGTGGCGATCTTGCGCAGGATGCGGCGCATGATCTTGCCCGAGCGGGTCTTCGGCAGCCCCGGCGCCCACTGGATCACATCCGGCGAAGCAATCGG‑GCCGATCTCCTTGCGCACCCAGTTCTTCAGCTCCAGGCGCAATTGCTc                                 >  6:262211/1‑149 (MQ=255)                                                                                                      aCCGGTGGCGATCTTGCGCAGGATGCGGCGCATGATCTTGCCCGAGCGGGTCTTCGGCAGCCCCGGCGCCCACTGGATCACATCCGGCGAAGCAATCGG‑GCCGATCTCCTTgcgc                                                                 <  3:242559/115‑1 (MQ=255)                                                                                                      aCCGGTGGCGATCTTGCGCAGGATGCGGCGCATGATCTTGCCCGAGCGGGTCTTCGGCAGCCCCGGCGCCCACTGGATCACATCCGGCGAAGCAATCGG‑GCCGATCTCCTTgcgc                                                                 >  4:242559/1‑115 (MQ=255)                                                                                                      aCCGGTGGCGATCTTGCGCAGGATGCGGCGCATGATCTTGCCCGAGCGGGTCTTCGGCAGCCCCGGCGCCCACTGGATCACATCCGGCGAAGCAATCGG‑GCCGATCTCCTTGCGCACCCAGTTCTTCAGCTCCAGGCGCAATTGCTCGc                               >  6:372068/1‑149 (MQ=255)                                                                                                      aCCGGTGGCGATCTTGCGCAGGATGCGGCGCATGATCTTGCCCGAGCGGGTCTTCGGCAGCCCCGGCGCCCACTGGATCACATCCGGCGAAGCAATCGG‑GCCGATCTCCTTGCGCACCCAGTTCTTCAGCTCCAGGCGCAATTGCTCGc                               <  5:372068/149‑1 (MQ=255)                                                                                                      aCCGGTGGCGATCTTGCGCAGGATGCGGCGCATGATCTTGCCCGAGCGGGTCTTCGGCAGCCCCGGCGCCCACTGGATCACATCCGGCGAAGCAATCGG‑GCCGATCTCCTTGCGCACCCAGTTCTTCAGCTCCAGGCGCAATTGCTCGc                               >  5:120277/1‑149 (MQ=255)                                                                                                       ccGGTGGCGATCTTGCGCAGGATGCGGCGCATGATCTTGCCCGAGCGGGTCTTCGGCAGCCCCGGCGCCCACTGGATCACATCCGGCGAAGCAATCGG‑GCCGATCTCCTTGCGCACCCAGTTCTTCAGCTCCAGGCGCAATTGCTCGCt                              <  7:36020/149‑1 (MQ=255)                                                                                                       ccGGTGGCGATCTTGCGCAGGATGCGGCGCATGATCTTGCCCGAGCGGGTCTTCGGCAGCCCCGGCGCCCACTGGATCACATCCGGCGAAGCAATCGG‑GCCGATCTCCTTGCGCACCCAGTTCTTCAGCTCCAGGCGCAATTGCTCGCt                              <  3:269627/149‑1 (MQ=255)                                                                                                        cGGTGGCGATCTTGCGCAGGATGCGGCGCATGATCTTGCCCGAGCGGGTCTTCGGCAGCCCCGGCGCCCACTGGATCACATCCGGCGAAGCAATCGG‑GCCGATCTCCTTGCGCACCCAGTTCTTCAGCTCCAGGCGCAATTGCTCGCTg                             >  6:1680/1‑149 (MQ=255)                                                                                                         gggcaagATCATGCGCCGCATCCTGCGCAAGATCTTGCCCGAGCGGGTCTTCGGCAGCCCCGGCGCCCACTGGATCACATCCGGCGAAGCAATCGG‑GCCGATCTCCTTGCGCACCCAGTTCTTCAGCTCCAGGCGCAATTGCTCGCTgg                            >  6:37588/7‑149 (MQ=255)                                                                                                          gTGGCGATCTTGCGCAGGATGCGGCGCATGATCTTGCCCGAGCGGGTCTTCGGCAGCCCCGGCGCCCACTGGATCACATCCGGCGAAGCAATCGG‑GCCGATCTCCtt                                                                     <  2:357070/107‑1 (MQ=255)                                                                                                          gTGGCGATCTTGCGCAGGATGCGGCGCATGATCTTGCCCGAGCGGGTCTTCGGCAGCCCCGGCGCCCACTGGATCACATCCGGCGAAGCAATCGG‑GCCGATCTCCtt                                                                     >  1:357070/1‑107 (MQ=255)                                                                                                           tGGCGATCTTGCGCAGGATGCGGCGCATGATCTTGCCCGAGCGGGTCTTCGGCAGCCCCGGCGCCCACTGGATCACATCCGGCGAAGCAATCGG‑GCCGATCTCCTTGCGCACCCAGTTCTTCAGCTCCAGGCGCAATTGCTCGCTGGcc                          >  4:197581/1‑149 (MQ=255)                                                                                                           gcaagATCATGCGCCGCATCCTGCGCAAGATCTTGCCCGAGCGGGTCTTCGGCAGCCCCGGCGCCCACTGGATCACATCCGGCGAAGCAATCGG‑GCCGATCTCCTTGCGCACCCAGTTCTTCAg                                                   >  8:47302/5‑124 (MQ=38)                                                                                                           gcaagATCATGCGCCGCATCCTGCGCAAGATCTTGCCCGAGCGGGTCTTCGGCAGCCCCGGCGCCCACTGGATCACATCCGGCGAAGCAATCGG‑GCCGATCTCCTTGCGCACCCAGTTCTTCAg                                                   <  7:47302/120‑1 (MQ=38)                                                                                                               gATCATGCGCCGCATCCTGCGCAAGATCTTGCCCGAGCGGGTCTTCGGCAGCCCCGGCGCCCACTGGATCAGATACGGCGAAGCAATCGG‑GCCGATCACCTTGCGCACCCAGTTCTTCAGCTCCACGCGCAATTGCTCGCTGGCCtgca                      >  7:275051/1‑146 (MQ=255)                                                                                                                aTCTTGCGCAGGATGCGGCGCATGATCTTGCCCGAGCGGGTCTTCGGCAGCCCCGGCGCCCACTGGATCACATCCGGCGAAGCAATCGG‑GCCGATCTCCTTGCGCACCCAGTTCTTCAGCTCCAGGCGCAATTGCTCGCTGGCCTCGAt                     >  3:116865/1‑149 (MQ=255)                                                                                                                 tCTTGCGCAGGATGCGGCGCATGATCTTGCCCGAGCGGGTCTTCGGCAGCCCCGGCGCCCACTGGATCACATCCGGCGAAGCAATCGG‑GCCGATCTCCTTGCGCACCCAGTTCTTCAGCTCCAGGCGCAATTGCTCGCTGGCCTCGATg                    >  4:88050/1‑149 (MQ=255)                                                                                                                     gcgcAGGATGCGGCGCATGATCTTGCCCGAGCGGGTCTTCGGCAGCCCCGGCGCCCACTGGATCACATCCGGCGAAGCAATCGG‑GCCGATCTCCTTGCGCACCCAGTTCTTCAGCTCCAGGCGCAATTGCTCGCTGGCCTCGATGCCg                 <  4:352817/148‑1 (MQ=255)                                                                                                                       gcAGGATGCGGCGCATGATCTTGCCCGAGCGGGTCTTCGGCAGCCCCGGCGCCCACTGGATCACATCCGGCGAAGCAATCGG‑GCCGATCTCCTTGCGCACCCAGTTCTTCAGCTCCAGGCGCAATTGCTCGCTGGCCTCGATGCCGGCa              >  7:200519/1‑149 (MQ=255)                                                                                                                           gATGCGGCGCATGATCTTGCCCGAGCGGGTCTTCGGCAGCCCCGGCGCCCACTGGATCACATCCGGCGAAGCAATCGG‑GCCGATCTCCTTGCGCACCCAGTTCTTCAGCTCCAGGCGCTATTGCTCGCTGGCCTCGATGCCGGCATTGa          <  1:220561/149‑1 (MQ=255)                                                                                                                           gATGCGGCGCATGATCTTGCCCGAGCGGGTCTTCGGCAGCCCCGGCGCCCACTGGATCACATCCGGCGAAGCAATCGG‑GCCGATCTCCTTGCGCACCCAGTTCTTCAGCTCCAGGCGCAATTGCTCGCTGGCCTCGATGCCGGCATTGa          <  1:177854/149‑1 (MQ=255)                                                                                                                             tGCGGCGCATGATCTTGCCCGAGCGGGTCTTCGGCAGCCCCGGCGCCCACTGGATCACATCCGGCGAAGCAATCGG‑GCCGATCTCCTTGCGCACCCAGTTCTTCAGCTCCAGGCGCAATTGCTCGCTGGCCTCGATGCCGGCATTGAgg        <  3:211917/149‑1 (MQ=255)                                                                                                                             tGCGGCGCATGATCTTGCCCGAGCGGGTCTTCGGCAGCCCCGGCGCCCACTGGATCACATCCGGCGAAGCAATCGG‑GCCGATCTCCTTGCGCACCCAGTTCTTCAGCTCCAGGCGCAATTGCTCGCTGGCCTCGATGCCGGCATTGAgg        >  8:260568/1‑149 (MQ=255)                                                                                                                             tGCGGCGCATGATCTTGCCCGAGCGGGTCTTCGGCAGCCCCGGCGCCCACTGGATCACATCCGGCGAAGCAATCGG‑GCCGATCTCCTTGCGCACCCAGTTCTTCAGCTCCAGGCGCAATTGCTCGCTGGCCTCGATGCCGGCATTGAgg        <  3:23595/149‑1 (MQ=255)                                                                                                                                  cgcATGATCTTGCCCGAGCGGGTCTTCGGCAGCCCCGGCGCCCACTGGATCACATCCGGCGAAGCAATCGG‑GCCGATCTCCTTGCGCACCCAGTTCTTCAGCTCCAGGCGCAATTGCTCGCTGGCCTCGATGCCGGCATTGAGGGTGAc   <  7:183574/149‑1 (MQ=255)                                                                                                                                  cgcATGATCTTGCCCGAGCGGGTCTTCGGCAGCCCCGGCGCCCACTGGATCACATCCGGCGAAGCAATCGG‑GCCGATCTCCTTGCGCACCCAGTTCTTCAGCTCCAGGCGCAATTGCTCGCTGGCCTCAATGCCGGCATCGAggccagc   <  6:51098/149‑6 (MQ=255)                                                                                                                                   gcATGATCTTGCCCGAGCGGGTCTTCGGCAGCCCCGGCGCCCACTGGATCACATCCGGCGAAGCAATCGG‑GCCGATCTCCTTGCGCACCCAGTTCTTCAGCTCCAGGCGCAATTGCTCGCTGGCCTCGATGCCGGCATTGAGGGTGACa  >  6:307912/1‑149 (MQ=255)                                                                                                                                   gcAAGATCTTGCCCGAGCGGGTCTTCGGCAGCCCCGGCGCCCACTGGATCACATCCGGCGAAGCAATCGG‑GCCGATCTCCTTGCGCACCCAGTTCTTCAGCTCCAGGCGCAATTGCTCGCTGGCCTCGATGCCGGCATTGAGGGTGACa  <  5:37588/149‑1 (MQ=255)                                                                                                                                      |                                                                                                                                                   GTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCGGGTCAGCCAAGGTCGAGATATCGCCCAGGGCGTCGTACTCACCGGTGGCGATCTTGCGCAGGATGCGGCGCATGATCTTGCCCGAGCGGGTCTTCGGCAGCCCCGGCGCCCACTGGATCACATCCGGCGAAGCAATCGG‑GCCGATCTCCTTGCGCACCCAGTTCTTCAGCTCCAGGCGCAATTGCTCGCTGGCCTCGATGCCGGCATTGAGGGTGACA  >  NC_002947/5097299‑5097577

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑