Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 428,866 G→T 8.3% A47A (GCC→GCA)  PP_0351 ← transmembrane sensor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947428,8660GT 8.3% 145.4 / 3.3 48A47A (GCC→GCA) PP_0351transmembrane sensor
Reads supporting (aligned to +/- strand):  ref base G (21/23);  new base T (2/2);  total (23/25)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.85e-01

GCAGCGCGCCGAGCAGAAACGTGCCGGCCAGCCCGCCGCCAACCTTGCCAATGCGTCGCCGCAGGCTGGCGCGCGATTGCAGCAGCGCCTGGCGTGCCGGGCCAGCTGCCGCGCTAACGCGTTGGTCCATGGCGCCGAGCT‑GGCGCCAGGCACGTGCGTGCTCTTCGCTGGCGGCATGCCAGCGCATGAATTCGTTGCGTTCATCAGGCGTGCCGCTGCTCTCGCCAAGGCTGAGTTTCCAGGCAATCGCGGCTTCCAGGACCCGGGACGAAACCGGGAGGTTGC  >  NC_002947/428718‑429002
                                                                                                                                                     |                                                                                                                                        
gcagcGCGCCGAGCAGAAACGTGCCGGCCAGCCCGCCGCCAACCTTGCCAATGCGTCGCCGCAGGCTGGCGCGCGATTGCAGCAGCGCCTGGCGTGCCGGGCCAGCTGCCGCGCTAACGCGTTGGTCCATGGCGCCGAGCT‑GGCGCCAg                                                                                                                                          <  6:149292/149‑1 (MQ=255)
gcagcGCGCCGAGCAGAAACGTGCCGGCCAGCCCGCCGCCAACCTTGCCAATGCGTCGCCGCAGGCTGGCGCGCGATTGCAGCAGCGCCTGGCGTGCCGGGCCAGCTGCCGCGCTAACGCGTTGGTCCATGGCGCCGAGCT‑GGCGCCAg                                                                                                                                          <  3:46780/149‑1 (MQ=255)
gcagcGCGCCGAGCAGAAACGTGCCGGCCAGCCCGCCGCCAACCTTGCCAATGCGTCGCCGCAGGCTGGCGCGCGATTGCAGCAGCGCCTGGCGTGCCGGGCCAGCTGCCGCGCTAACGCGTTGGTCCATGGCGCCGAGCT‑GGCGCCAg                                                                                                                                          <  6:257101/149‑1 (MQ=255)
gcagcGCGCCGAGCAGAAACGTGCCGGCCAGCCCGCCGCCAACCTTGCCAATGCGTCGCCGCAGGCTGGCGCGCGATTGCAGCAGCGCCTGGCGTGCCGGGCCAGCTGCCGCGCTAACGCGTTGGTCCATGGCGCCGAGCT‑GGCGCCAg                                                                                                                                          <  3:313962/149‑1 (MQ=255)
     gcgcCGAGCAGAAACGTGCCGGCCAGCCCGCCGCCAACCTTGCCAATGCGTCGCCGCAGGCTGGCGCGCGATTGCAGCAGCGCCTGGCGTGCCGGGCCAGCTGCCGCGCTAACGCGTTGGTCCATGGCGCCGAGCT‑GGCGCCAGGCAcg                                                                                                                                     >  1:272366/1‑149 (MQ=255)
      cgcCGAGCAGAAACGTGCCGGCCAGCCCGCCGCCAACCTTGCCAATGCGGCGCCGCAGGCTGGCGCGCGATTGCAGCAGCGCCTGGCGTGCCGGGCCAGCTGCCGCGCTAACGCGTTGGTCCATGGCGCCGAGCT‑GGCGCCAGGCAcgt                                                                                                                                    <  1:67216/149‑1 (MQ=255)
        cctaGCAGAAAAGTGCCGGCCAGCCCGCCGCCAACCTTGCCAATGCGGCGCCGCAGGCTGGCGCGCGATTGCAGCAGCGCCTGGCGTGCCGGGCCAGCTGCCGCGCTAACGCGTTGGTCCATGGCGCCGAGCT‑GGCGCCAGGCAcgtgc                                                                                                                                  <  8:246615/146‑1 (MQ=255)
           aGCAGAAACGTGCCGGCCAGCCCGCCGCCAACCTTGCCAATGCGTCGCCGCAGGCTGGCGCGCGATTGCAGCAGCGCCTGGCGTGCCGGGCCAGCTGCCGCGCTAACGCGTTGGTCCATGGCGCCGAGCT‑GGCGCCAGGCAcgtgcgtg                                                                                                                               <  8:103725/149‑1 (MQ=255)
               gAAACGTGCCGGCCAGCCCGCCGCCAACCTTGCCAATGCGTCGCCGCAGGCTGGCGCGCGATTGCAGCAGCGCCTGGCGTGCCGGGCCAGCTGCCGCGCTAACGCGTTGGTCCATGGCGCCGAGCT‑GGCGCCAGGCACGTGCGTGctct                                                                                                                           >  8:203534/1‑149 (MQ=255)
                 aaCGTGCCGGCCAGCCCGCCGCCAACCTTGCCAATGCGTCGCCGCAGGCTGGCGCGCGATTGCAGCAGCGCCTGGCGTGCCGGGCCAGCTGCCGCGCTAACGCGTTGGTCCATGGCGCCGAGCT‑GGCGCCAGGCACGTGCGTGCTCTTc                                                                                                                         <  5:261822/149‑1 (MQ=255)
                       ccGGCCAGCCCGCCGCCAACCTTGCCAATGCGTCGCCGCAGGCTGGCGCGCGATTGCAGCAGCGCCTGGCGTGCCGGGCCAGCTGCCGCGCTAACGCGTTGGTCCATGGCGCCGAGCT‑GGCGCCAGGCAcgtgcgt                                                                                                                                <  7:245541/136‑1 (MQ=255)
                       ccGGCCAGCCCGCCGCCAACCTTGCCAATGCGTCGCCGCAGGCTGGCGCGCGATTGCAGCAGCGCCTGGCGTGCCGGGCCAGCTGCCGCGCTAACGCGTTGGTCCATGGCGCCGAGCT‑GGCGCCAGGCAcgtgcgt                                                                                                                                >  8:245541/1‑136 (MQ=255)
                               cccGCCGCCAACCTTGCCAATGCGTCGCCGCAGGCTGGCGCGCGATTGCAGCAGCGCCTGGCGTGCCGGGCCAGCTGCCGCGCTAACGCGTTGGTCCATGGCGCCGAGCT‑GGCGCCAGGCACGTGCGTGCTCTGCGCTggcatgccgc                                                                                                            >  6:110216/1‑141 (MQ=255)
                                ccgccgCCAACCTTGCCAATGCGTCGCCGCAGGCTGGCGCGCGATTGCAGCAGCGCCTGGCGTGCCGGGCCAGCTGCCGCGCTAACGCGTTGGTCCATGGCGCCGAGCT‑GGCGCCAGGCACGTGCGTGCTCTTCGCTGGCGGCATGc                                                                                                            >  5:292606/1‑147 (MQ=255)
                                 cgccgcCAACCTTGCCAATGCGTCGCCGCAGGCTGGCGCGCGATTGCAGCAGCGCCTGGCGTGCCGGGCCAGCTGCCGCGCTAACGCGTTGGTCCATGGCGCCGAGCT‑GGCGCCAGGCACGTGCGAGCTCTTCGCTGGCGGCATGCCAg                                                                                                         >  7:339128/1‑149 (MQ=255)
                                    cgccAACCTTGCCAATGCGTCGCCGCAGGCTGGCGCGCGATTGCAGCAGCGCCTGGCGTGCCGGGCCAGCTGCCGCGCTAACGCGTTGGTCCATGGCGCCGAGCT‑GGCGCCAGGCACGTGCGTGCTCTTCGCTGGCGGCATGCCAgcgc                                                                                                      <  7:116701/149‑1 (MQ=255)
                                     gccAACCTTGCCAATGCGTCGCCGCAGGCTGGCGCGCGATTGCAGCAGCGCCTGGCGTGCCGGGCCAGCTGCCGCGCTAACGCGTTGGTCCATGGCGCCGAGCT‑GGCGCCAGGCACGTGCGTGCTCTTCGCTGGCGGCATGCCAGCGCa                                                                                                     <  2:253385/149‑1 (MQ=255)
                                      ccAACCTTGCCAATGCGTCGCCGCAGGCTGGCGCGCGATTGCAGCAGCGCCTGGCGTGCCGGGCCAGCTGCCGCGCTAACGCGTTGGTCCATGGCGCCGAGCT‑GGCGCCAGGCACGTGCGTGCTCTTCGCTGGCGGCATGCCAGCGCAt                                                                                                    >  7:53413/1‑149 (MQ=255)
                                               ccAATGCGTCGCCGCAGGCTGGCGCGCGATTGCAGCAGCGCCTGGCGTGCCGGGCCAGCTGCCGCGCTAACGCGTTGGTCCATGGCGCCGAGCT‑GGCGCCAGGCACGTGCGTGCTCTTCGCTGGCGGCATGCCAGCGCATGAATTCGtt                                                                                           <  2:272366/149‑1 (MQ=255)
                                                          ccgcAGGCTGGCGCGCGATTGCAGCAGCGCCTGGCGTGCCGGGCCAGCTGCCGCGCTAACGCGTTGGTCCATGGCGCCGAGCT‑GGCGCCAGGCACGTGCGTGCTCTTCGCTGGCGGCATGCCAGCGCATGAATTCGTTGCGTTCATCAg                                                                                <  6:292606/149‑1 (MQ=255)
                                                          ccgcAGGCTGGCGCGCGATTGCAGCAGCGCCTGGCGTGCCGGGCCAGCTGCCGCGCTAACGCGTTGGTCCATGGCGCCGAGCT‑GGCGCCAGGCACGTGCGTGCTCTTCGCTGGCGGCATGCCAGCGCATGAATTCGTTGCGTTCATCAg                                                                                >  6:163929/1‑149 (MQ=255)
                                                              aGGCTGGCGCGCGATTGCAGCAGCGCCTGGCGTGCCGGGCCAGCTGCCGCGCTAACGCGTTGGTCCATGGCGCCGAGCT‑GGCGCCAGGCACGTGCGTGCTCTTCGCTGGCGGCATGCCAGCGCATGAATTCGTTGCGTTCATCAGGCGt                                                                            <  8:177642/149‑1 (MQ=255)
                                                                gCTGGCGCGCGATTGCAGCAGCGCCTGGCGTGCCGGGCCAGCTGCCGCGCTAACGCGTTGGTCCATGGCGCCGAGCT‑GGCGCCAGGCACGTGCGTGCTCTTCGCTGGCGGCATGCCAGCGCATGAATTCGTTGCGTTCATCAGGCGTGc                                                                          >  1:134818/1‑149 (MQ=255)
                                                                              gcagcaGCGCCTGGCGTGCCGGGCCAGCTGCCGCGCTAACGCGTTGGTCCATGGCGCCGAGCT‑GGCGCCAGGCACGTGCGTGCTCTTCGCTGGCGGCATGCCAGCGCATGAATTCGTTGCGTTCATCAGGCGTGCCGCTGCTCTCGcc                                                             >  3:70999/1‑148 (MQ=255)
                                                                               cagcagCGCCTGGCGTGCCGGGCCAGCTGCCGCGCTAACGCGTTGGTCCATGGCGCCGAGCT‑GGCGCCAGGCACGTGCGTGCTCTTCGCTGGCGGCATGCCAGCGCATGAATTCGTTGCGTTCATCAGGCGTGCCGCTGCTCTCGcc                                                             >  3:309036/1‑147 (MQ=255)
                                                                                  cagcGCCTGGCGTGCCGGGCCAGCTGCCGCGCTAACGCGTTGGTCCATGGCGCCGAGCT‑GGCGCCAGGCACGTGCGTGCTCTTCGCTGGCGGCATGCCAGCGCATGAATTCGTTGCGTTCATCAGGCGTGCCGCTGCTCTCGCCAAGGc                                                        >  8:123872/1‑149 (MQ=255)
                                                                                           gCGTGCCGGGCCAGCTGCCGCGCTAACGCGTTGGTCCATGGCGCCGAGCT‑GGCGCCAGGCACGTGCGTGCTCTTCGCTGGCGGCATGCCAGCGCATGAATTCGTTGCGTTCATCAGGCGTGCCGCTGCTCTCGCCAAGGCTGAGTTTc                                                >  2:341558/1‑148 (MQ=255)
                                                                                            cGTGCCGGGCCAGCTGCCGCGCTAACGCGTTGGTCCATGGCGCCGAGCT‑GGCGCCAGGCACGTGCGTGCTCTTCGCTGGCGGCATGCCAGCGCATGAATTCGTTGCGTTCATCAGGCGTGCCGCTGCTCTCGCCAAGGCTGAGTTTCCa                                              <  7:203534/149‑1 (MQ=255)
                                                                                             gTGCCGGGCCAGCTGCCGCGCTAACGCGTTGGTCCATGGCGCCGAGCT‑GGCGCCAGGCACGTGCGTGCTCTTCGCTGGCGGCATGCCAGCGCATGAATTCGTTGCGTTCATCAGGCGTGCCGCTGCTCTCGCCAAGGCTGAGTTTCCAg                                             <  5:163929/149‑1 (MQ=255)
                                                                                               gCCGGGCCAGCTGCCGCGCTAACGCGTTGGTCCATGGCGCCGAGCT‑GGCGCCAGGCACGTGCGTGCTCTTCGCTGGCGGCATGCCAGCGCATGAATTCGTTGCGTTCATCAGGCGTGCCGCTGCTCTCGCCAAGGCTGAGTTTCCAgg                                            >  5:314260/1‑148 (MQ=255)
                                                                                               gCCGGGCCAGCTGCCGCGCTAACGCGTTGGTCCATGGCGCCGAGCT‑GGCGCCAGGCACGTGCGTGCTCTTCGCTGGCGGCATGCCAGCGCATGAATTCGTTGCGTTCATCAGGCGTGCCGCTGCTCTCGCCAAGGCTGAGTTTCCAgg                                            >  3:10287/1‑148 (MQ=255)
                                                                                                     ccAGCTGCCGCGCTAACGCGTTGGTCCATGGCGCCGAGCT‑GGCGCCAGGCACGTGCGTGCTCTTCGCTGGCGGCATGCCAGCGCATGAATTCGTTGCGTTCATCAGGCGTGCCGCTGCTCTCGCCAAGGCTGAGTTTCCAGGCAATcgc                                     <  7:123872/149‑1 (MQ=255)
                                                                                                     ccAGCTGCCGCGCTAACGCGTTGGTCCATGGCGCCGAGCT‑GGCGCCAGGCACGTGCGTGCTCTTCGCTGGCGGCATGCCAGCGCATGAATTCGTTGCGTTCATCAGGCGTGCCGCTGCTCTCGCCAAGGCTGAGTTTCCAGGCAATcgc                                     <  4:309036/149‑1 (MQ=255)
                                                                                                      cAGCTGCCGCGCTAACGCGTTGGTCCATGGCGCCGAGCT‑GGCGCCAGGCACGTGCGTGCTCTTCGCTGGCGGCATGCCAGCGCATGAATTCGTTGCGTTCATCAGGCGTGCCGCTGCTCTCGCCAAGGCTGAGTTTCCAGGCAATcgcg                                    >  1:11048/1‑149 (MQ=255)
                                                                                                         cTGCCGCGCTAAGGCGTTGGTCCATGGCGCCGAGCT‑GGCGCCAGGCACGTGCGTGCTCTTCGCTGGCGGCATGCCAGCGCATGAATTCGTTGCGTTCATCAGGCGTGCCGCTGCTCTCGCCAAGGCTGAGTTTCCAGGCAATCGCGGCt                                 <  3:353722/149‑1 (MQ=255)
                                                                                                            ccGCGCTAACGCGTTGGTCCATGGCGCCGAGCT‑GGCGCCAGGCACGTGCGTGCTCTTCGCTGGCGGCATGCCAGCGCATGAATTCGTTGCGTTCATCAGGCGTGCCGCTGCTCTCGCCAAGGCTGAGTTTCCAGGCAATCGCGGCTTcc                              <  1:365650/149‑1 (MQ=255)
                                                                                                              gcgcTAACGCGTTGGTCCATGGCGCCGAGCT‑GGCGCCAGGCACGTGCGTGCTCTTCGCTGGCGGCATGCCAGCGCATGAATTCGTTGCGTTCATCAGGCGTGCCGCTGCTCTCGCCAAGGCTGAGTTTCCAGGCAATCGCGGCTTCCAg                            >  4:285351/1‑149 (MQ=255)
                                                                                                                    aCGCGTTGGTCCATGGCGCCGAGCT‑GGCGCCAGGCACGTGCGTGCTCTTCGCTGGCGGCATGCCAGCGCATGAATTCGTTGCGTTCATCAGGCGTGCCGCTGCTCTCGCCAAGGCTGAGTTTCCAGGCAATCGCGGCTTCCAGGACCCg                      <  4:70999/149‑1 (MQ=255)
                                                                                                                        gTTGGTCCATGGCGCCGAGCT‑GGCGCCAGGCACGTGCGTGCTCTTCGCTGGCGGCATGCCAGCGCATGAATTCGTTGCGTTCATCAGGCGTGCCGCTGCTCTCGCCAAGGCTGAGTTTCCAg                                             <  4:119635/122‑1 (MQ=255)
                                                                                                                        gTTGGTCCATGGCGCCGAGCT‑GGCGCCAGGCACGTGCGTGCTCTTCGCTGGCGGCATGCCAGCGCATGAATTCGTTGCGTTCATCAGGCGTGCCGCTGCTCTCGCCAAGGCTGAGTTTCCAg                                             >  3:119635/1‑122 (MQ=255)
                                                                                                                        gTTGGTCCATGGCGCCGAGCT‑GGCGCCAGGCACGTGCGTGCTCTTCGCTGGCGGCATGCCAGCGCATGAATTCGTTGCGTTCATCAGGCGTGCCGCTGCTCTCGCCAAGGCTGAGTTTCCAGGCAATCGCGGCTTCCAGGACCCGGGAc                  <  2:134818/149‑1 (MQ=255)
                                                                                                                            gtgcctGGCGCC‑AGCTCGGCGCCATGCACGTGCGTGCTCTTCGCTGGCGGCATGCCAGCGCATGAATTCGTTGCGTTCATCAGGCGTGCCGCTGCTCTCGCCAAGGCTGAGTTTCCAGGCAATCGCGGCTTCCAGGACACGGGACGaaa              >  6:32653/6‑149 (MQ=255)
                                                                                                                            gtgcctGGCGCC‑AGCTCGGCGCCATGCACGTGCGTGCTCTTCGCTGGCGGCATGCCAGCGCATGAATTCGTTGCGTTCATCAGGCGTGCCGCTGCTCTCGCCAAGGCTGAGTTTCCAGGCAATCGCGGCTTCCAGGACACGGGACGaaa              >  6:27153/6‑149 (MQ=255)
                                                                                                                             tCCATGGCGCCGAGCT‑GGCGCCAGGCACGTGCGTGCTCTTCGCTGGCGGCATGCCAGCGCATGAATTCGTTGCGTTCATCAGGCGTGCCGCTGCTCTCGCCAAGGCTGAGTTTCCAGGCAATCGCGGCTTCCAGGACCCGGGACGAAAc             >  3:364556/1‑149 (MQ=255)
                                                                                                                             tCCATGGCGCCGAGCT‑GGCGCCAGGCACGTGCGTGCTCTTCGCTGGCGGCATGCCAGCGCATGAATTCGTTGCGTTCATCAGGCGTGCCGCTGCTCTCGCCAAGGCTGAGTTTCCAGGCAATCGCGGCTTCCAGGACCCGGGACGAAAc             >  2:134402/1‑149 (MQ=255)
                                                                                                                                 tGGCGCCGAGCT‑GGCGCCAGGCACGTGCGTGCTCTTCGCTGGCGGCATGCCAGCGCATGAATTCGTTGCGTTCATCAGGCGTGCCGCTGCTCTCGCCAAGGCTGAGTTTCCAGGCAATCGCGGCTTCCAGGACCCGGGACGAAACCggg         <  2:11048/149‑1 (MQ=255)
                                                                                                                                 tGGCGCCGAGCT‑GGCGCCAGGCACGTGCGTGCTCTTCGCTGGCGGCATGCCAGCGCATGAATTCGTTGCGTTCATCAGGCGTGCCGCTGCTCTCGCCAAGGCTGAGTTTCCAGGCAATCGCGGCTTCCAGGACCCGGGACGAAACCggg         <  8:53413/149‑1 (MQ=255)
                                                                                                                                   gcgcCGAGCT‑GGCGCCAGGCACGTGCGTGCTCTTCGCTGGCGGCATGCCAGCGCATGAATTCGTTGCGTTCATCAGGCGTGCCGCTGCTCTCGCCAAGGCTGAGTTTCCAGGCAATCGCGGCTTCCAGGACCCGGGACGAAACCGGGa        <  8:339128/148‑1 (MQ=255)
                                                                                                                                     gcCGAGCT‑GGCGCCAGGCACGTGCGTGCTCTTCGCTGGCGGCATGCCAGCGCATGAATTCGTTGCGTTCATCAGGCGTGCCGCTGCTCTCGCCAAGGCTGAGTTTCCAGGCAATCGCGGCTTCCAGGACCCGGGACGaaa              <  3:227105/140‑1 (MQ=255)
                                                                                                                                     gcCGAGCT‑GGCGCCAGGCACGTGCGTGCTCTTCGCTGGCGGCATGCCAGCGCATGAATTCGTTGCGTTCATCAGGCGTGCCGCTGCTCTCGCCAAGGCTGAGTTTCCAGGCAATCGCGGCTTCCAGGACCCGGGACGaaa              >  4:227105/1‑140 (MQ=255)
                                                                                                                                         agctcggCGCCATGCACGTGCGTGCTCTTCGCTGGCGGCATGCCAGCGCATGAATTCGTTGCGTTCATCAGGCGTGCCGCTGCTCTCGCCAAGGCTGAGTTTCCAGGCAATCGCGGCTTCCAGGACACGGGACGAAACCGGGAGGTTGc  <  5:32653/144‑1 (MQ=255)
                                                                                                                                         agctcggCGCCATGCACGTGCGTGCTCTTCGCTGGCGGCATGCCAGCGCATGAATTCGTTGCGTTCATCAGGCGTGCCGCTGCTCTCGCCAAGGCTGAGTTTCCAGGCAATCGCGGCTTCCAGGACACGGGACGAAACCGGGAGGTTGc  <  5:27153/144‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                        
GCAGCGCGCCGAGCAGAAACGTGCCGGCCAGCCCGCCGCCAACCTTGCCAATGCGTCGCCGCAGGCTGGCGCGCGATTGCAGCAGCGCCTGGCGTGCCGGGCCAGCTGCCGCGCTAACGCGTTGGTCCATGGCGCCGAGCT‑GGCGCCAGGCACGTGCGTGCTCTTCGCTGGCGGCATGCCAGCGCATGAATTCGTTGCGTTCATCAGGCGTGCCGCTGCTCTCGCCAAGGCTGAGTTTCCAGGCAATCGCGGCTTCCAGGACCCGGGACGAAACCGGGAGGTTGC  >  NC_002947/428718‑429002

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑