Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,244,820 G→A 7.5% G76E (GGG→GAG)  hmgR → Hmg transcriptional repressor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,244,8200GA 7.5% 154.8 / 2.2 53G76E (GGG→GAG) hmgRHmg transcriptional repressor
Reads supporting (aligned to +/- strand):  ref base G (27/22);  new base A (2/2);  total (29/24)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CAGAACTCTCACCCTCGACGTCACTATCACGCCTGGCCGAGCACGTGCAGATGCCTGCGAGCAAAGTGCATCGCTACCTGCAGGCGCTGATTGCCAGTGGTTTTGCCGAACAGGATGCGGCCACCAACCATTACGGGCTGGGGCGCGAGGCATTGCGGGTGGGGCTGGCGGCGCTGGGCAGCATCGATGTGCTGAAGGTGGCGGCGATGCCGTTGTCGCAGTTGCGAGATGAGCTCAACGAAAGCTGCTTCATCGCCGTGTGGGGCAACCAGGGGGCCAC  >  NC_002947/5244679‑5244958
                                                                                                                                             |                                                                                                                                          
cAGAACTCTCACCCTCGACGTCACTATCACGCCTGGCCGAGCACGTGCAGATGCCTGCGAGCAAAGTGCATCGCTACCTGCAGGTGCTGATTGCCAGTGGTTTTGCCGAACAGGATGCGGCCACCAACCATTACGGGCTGGGGCGCGAg                                                                                                                                     <  3:157023/149‑1 (MQ=255)
      tctcACCCTCGACGTCACTATCACGCCTGGCCGAGCACGTGCAGATGCCTGCGAGCAAAGTGCATCGCTACCTGCAGGCGCTGATTGCCAGTGGTTTTGCCGAACAGGATGCGGCCACCAACCATTACGGGCTGGGGCGCGAGGCATTg                                                                                                                               >  1:358669/1‑149 (MQ=255)
      tctcACCCTCGACGTCACTATCACGCCTGGCCGAGCACGTGCAGATGCCTGCGAGCAAAGTGCATCGCTACCTGCAGGCGCTGATTGCCAGTGGTTTTGCCGAACAGGATGCGGCCACCAACCATTACGGGCTGGGGCGCGAGGCATTg                                                                                                                               >  2:369550/1‑149 (MQ=255)
        tcACCCTCGACGTCACTATCACGCCTGGCCGAGCACGTGCAGATGCCTGCGAGCAAAGTGCATCGCTACCTGCAGGCGCTGATTGCCAGTGGTTTTGCCGAACAGGATGCGGCCACCAACCATTACGGGCTGGGGCGCGAGGCATTGCg                                                                                                                             >  3:175997/1‑149 (MQ=255)
            ccTCGACGTCACTATCACGCCTGGCCGAGCACGTGCAGATGCCTGCGAGCAAAGTGCATCGCTACCTGCAGGCGCTGATTGCCAGTGGTTTTGCCGAACAGGATGCGGCCACCAACCATTACGGGCTGGGGCGCGAGGCATTGCGGGTg                                                                                                                         >  3:354449/1‑149 (MQ=255)
            ccTCGACGTCACTATCACGCCTGGCCGAGCACGTGCAGATGCCTGCGAGCAAAGTGCATCGCTACCTGCAGGCGCTGATTGCCAGTGGTTTTGCCGAACAGGATGCGGCCACCAACCATTACGGGCTGGGGCGCGAGGCATTGCGGGTg                                                                                                                         >  7:107906/1‑149 (MQ=255)
            ccTCGACGTCACTATCACGCCTGGCCGAGCACGTGCAGATGCCTGCGAGCAAAGTGCATCGCTACCTGCAGGCGCTGATTGCCAGTGGTTTTGCCGAACAGGATGCGGCCACCAACCATTACGGGCTGGGGCGCGAGGCATTGCGGGTg                                                                                                                         <  1:369550/149‑1 (MQ=255)
                gACGTCACTATCACGCCTGGCCGAGCACGTGCAGATGCCTGCGAGCAAAGTGCATCGCTACCTGCAGGCGCTGATTGCCAGTGGTTTTGCCGAACAGGATGCGGCCACCAACCATTACGGGCTGGGGCGCGAGGCATTGCGGGTGGGGc                                                                                                                     <  2:358669/149‑1 (MQ=255)
                  cGTCACTATCACGCCTGGCCGAGCACGTGCAGATGCCTGCGAGCAAAGTGCATCGCTACCTGCAGGCGCTGATTGCCAGTGGTTTTGCCGAACAGGATGCGGCCACCAACCATTACGGGCTGGGGCGCGAGGCATTGCGGGTGGGGCTg                                                                                                                   <  7:240801/149‑1 (MQ=255)
                          tCACGCCTGGCCGAGCACGTGCAGATGCCTGCGAGCAAAGTGCATCGCTACCTGCAGGCGCTGATTGCCAGTGGTTTTGCCGAACAGGATGCGGCCACCAACCATTACGGGCTGGGGCGCGAGGCATTGCGGGTGGGGCTGGCGGCGCt                                                                                                           >  2:112552/1‑149 (MQ=255)
                           cACGCCTGGCCGAGCACGTGCAGATGCCTGCGAGCAAAGTGCATCGCTACCTGCAGGCGCTGATTGCCAGTGGTTTTGCCGAACAGGATGCGGCCACCAACCATTACGGGCTGGGGCGCGAGGCATTGCGGGTGGGGCTGGCGGCGCTg                                                                                                          >  4:338806/1‑149 (MQ=255)
                              gCCTGGCCGAGCACGTGCAGATGCCTGCGAGCAAAGTGCATCGCTACCTGCAGGCGCTGATTGCCAGTGGTTTTGCCGAACAGGATGCGGCCACCAACCATTACGGGCTGGGGCGCGAGGCATTGCGGGTGGGGCTGGCGGCGCTggg                                                                                                        >  8:213772/1‑148 (MQ=255)
                               ccTGGCCGAGCACGTGCAGATGCCTGCGAGCAAAGTGCATCGCTACCTGCAGGCGCTGATTGCCAGTGGTTTTGCCGAACAGGATGCGGCCACCAACCATTACGGGCTGGGGCGCGAGGCATTGCGGGTGGGGCTGGCGGCGCTGGgca                                                                                                      <  1:36243/149‑1 (MQ=255)
                                cTGGCCGAGCACGTGCAGATGCCTGCGAGCAAAGTGCATCGCTACCTGCAGGCGCTGATTGCCAGTGGTTTTGCCGAACAGGATGCGGCCACCAACCATTACGGGCTGGGGCGCGAGGCATTGCGGGTGGGGCTGGCGGCGCTGGgcag                                                                                                     >  5:65168/1‑149 (MQ=255)
                                 tGGCCGAGCACGTGCAGATGCCTGCGAGCAAAGTGCATCGCTACCTGCAGGCGCTGATTGCCAGTGGTTTTGCCGAACAGGATGCGGCCACCAACCATTACGGGCTGGGGCGCGAGGCATTGCGGGTGGGGCTGGCTGCGCTGGgcag                                                                                                     >  6:356975/1‑148 (MQ=255)
                                   gCCGAGCACGTGCAGATGCCTGCGAGCAAAGTGCATCGCTACCTGCAGGCGCTGATTGCCAGTGGTTTTGCCGAACAGGATGCGGCCACCAACCATTACGGGCTGGGGCGCGAGGCATTGCGGGTGGGGCTGGCGGCGCTGGGCAGCAt                                                                                                  <  4:175997/149‑1 (MQ=255)
                                   gCCGAGCACGTGCAGATGCCTGCGAGCAAAGTGCATCGCTACCTGCAGGCGCTGATTGCCAGTGGTTTTGCCGAACAGGATGCGGCCACCAACCATTACGGGCTGGAGCGCGAGGCATTGCGGGTGGGGCTGGCGGCGCTGGGCAGCAt                                                                                                  >  6:42190/1‑149 (MQ=255)
                                   gCCGAGCACGTGCAGATGCCTGCGAGCAAAGTGCATCGCTACCTGCAGGCGCTGATTGCCAGTGGTTTTGCCGAACAGGATGCGGCCACCAACCATTACGGGCTGGAGCGCGAGGCATTGCGGGTGGGGCTGGCGGCGCTGGGCAGCAt                                                                                                  >  6:36714/1‑149 (MQ=255)
                                                 gATGCCTGCGAGCAAAGTGCATCGCTACCTGCAGGCGCTGATTGCCAGTGGTTTTGCCGAACAGGATGCGGCCACCAACCATTACGGGCTGGGGCGCGAGGCATTGCGGGTGGGGCTGGCGGCGCTGGGCAGCATCGATGTGCTGAAgg                                                                                    >  7:159331/1‑149 (MQ=255)
                                                      cTGCGAGCAAAGTGCATCGCTACCTGCAGGCGCTGATTGCCAGTGGTTTTGCCGAACAGGATGCGGCCACCAACCATTACGGGCTGGGGCGCGAGGCATTGCGGGTGGGGCTGGCGGCGCTGGGCAGCATCGATGTGCGGAAGGTggcg                                                                               >  7:69253/1‑149 (MQ=255)
                                                        gCGAGCAAAGTGCATCGCTACCTGCAGGCGCTGATTGCCAGTGGTTTTGCCGAACAGGATGCGGCCACCAACCATTACGGGCTGGGGCGCGAGGCATTGCGGGTGGGGCTGGCGGCGCTGGGCAGCATCGATGTGCTGAAGGTggcggc                                                                             >  1:349080/1‑149 (MQ=255)
                                                             cAAAGTGCATCGCTACCTGCAGGCGCTGATTGCCAGTGGTTTTGCCGAACAGGATGCGGCCACCAACCATTACGGGCTGGGGCGCGAGGCATTGCGGGTggg                                                                                                                       >  7:122518/1‑102 (MQ=255)
                                                             cAAAGTGCATCGCTACCTGCAGGCGCTGATTGCCAGTGGTTTTGCCGAACAGGATGCGGCCACCAACCATTACGGGCTGGGGCGCGAGGCATTGCGGGTggg                                                                                                                       <  8:122518/102‑1 (MQ=255)
                                                              aaaGTGCATCGCTACCTGCAGGCGCTGATTGCCAGTGGTTTTGCCGAACAGGATGCGGCCACCAACCATTACGGGCTGGGGCGCGAGGCATTGCGGGTGGGGCTGGCGGCGCTGGGCAGCATCGATGTGCTGAAGGTGGCGGCGATGcc                                                                       <  3:338806/149‑1 (MQ=255)
                                                                 gTGCATCGCTACCTGCAGGCGCTGATTGCCAGTGGTTTTGCCGAACAGGATGCGGCCACCAACCATTACGGGCTGGGGCGCGAGGCATTGCGGGTGGGGCTGGCGGCGCTGGGCAGCATCGATGTGCTGAAGGTGGCGGCGATGCCGtt                                                                    <  1:112552/149‑1 (MQ=255)
                                                                   gCATCGCTACCTGCAGGCGCTGATTGCCAGTGGTTTTGCCGAACAGGATGCGGCCACCAACCATTACGGGCTGGAGCGCGAGGCATTGCGGGTGGGGCTGGCGGCGCTGGGCAGCATCGATGTGCTGAAGGTGGCGGCGATGCCGTTGt                                                                  <  5:36714/149‑1 (MQ=255)
                                                                    cATCGCTACCTGCAGGCGCTGATTGCCAGTGGTTTTGCCGAACAGGATGCGGCCACCAACCATTACGGGCTGGAGCGCGAGGCATTGCGGGTGGGGCTGGCGGCGCTGGGCAGCATCGATGTGCTGAAGGTGGCGGCGATGCCGTTGt                                                                  <  5:42190/148‑1 (MQ=255)
                                                                     aTCGCTACCTGCAGGCGCTGATTGCCAGTGGTTTTGCCGAACAGGATGCGGCCACCAACCATTACGGGCTGGGGCGCGAGGCATTGCGGGTGGGGCTGGCGGCGCTGGGCAGCATCGATGTGCTGAAGGTGGCGGCGATGCCGTTGTCg                                                                >  3:131587/1‑149 (MQ=255)
                                                                     aTCGCTACCTGCAGGCGCTGATTGCCAGTGGTTTTGCCGAACAGGATGCGGCCACCAACCATTACGGGCTGGGGCGCGAGGCATTGCGGGTGGGGCTGGCGGCGCTGGGCAGCATCGATGTGCTGAAGGTGGCGGCGATGCCGTTGTCg                                                                >  4:157244/1‑149 (MQ=255)
                                                                            ccTGCAGGCGCTGATTGCCAGTGGTTTTGCCGAACAGGATGCGGCCACCAACCATTACGGGCTGGGGCGCGAGGCATTGCGGGTGGGGCTGGCGGCGCTGGGCAGCATCGATGTGCTGAAGGTGGCGGCGATGCCGTTGTCGCAGTTg                                                          <  7:213772/148‑1 (MQ=255)
                                                                            ccTGCAGGCGCTGATTGCCAGTGGTTTTGCCGAACAGGATGCGGCCACCAACCATTACGGGCTGGGGCGCGAGGCATTGCGGGTGGGGCTGGCGGCGCTGGGCAGCATCGATGTGCTGAAGGTGGCGGCGATGCCGTTGTCGCAGTTGc                                                         >  5:57665/1‑149 (MQ=255)
                                                                              tGCAGGCGCTGATTGCCAGTGGTTTTGCCGAACAGGATGCGGCCACCAACCATTACGGGCTGGGGCGCGAGGCATTGCGGGTGGGGCTGGCGGCGCTGGGCAGCATCGAtgtg                                                                                           >  8:93213/1‑113 (MQ=255)
                                                                              tGCAGGCGCTGATTGCCAGTGGTTTTGCCGAACAGGATGCGGCCACCAACCATTACGGGCTGGGGCGCGAGGCATTGCGGGTGGGGCTGGCGGCGCTGGGCAGCATCGAtgtg                                                                                           <  7:93213/113‑1 (MQ=255)
                                                                                cAGGCGCTGATTGCCAGTGGTTTTGCCGAACAGGATGCGGCCACCAACCATTACGGGCTGGGGCGCGAGGCATTGCGGGTGGGGCTGGCGGCGCTGGGCAGCATCGATGTGCTGAAGGTGGCGGCGATGCCGTTGTCGCAGTTGCgag                                                      <  8:107906/148‑1 (MQ=255)
                                                                                               aGTGGTTTTGCCGAACAGGATGCGGCCACCAACCATTACGGGCTGGGGCGCGAGGCATTGCGGGTGGGGCTGGCGGCGCTGGGCAGCATCGATGTGCTGAAGGTGGCGGCGATGCCGTTGTCGCAGTTGCGAGATGAGCTCAACGAAAg                                      >  4:178064/1‑149 (MQ=255)
                                                                                                 tGGTTTTGCCGAACAGGATGCGGCCACCAACCATTACGGGCTGGGGCGCGAGGCATTGCGGGTGGGGCTGGCGGCGCTGGGCAGCATCGATGTGCTGAAGGTGGCGGCGATGCCGTTGTCGCAGTTGCGAGATGAGCTCAACGAAAgc                                     <  4:354449/148‑1 (MQ=255)
                                                                                                   gTTTTGCCGAACAGGATGCGGCCACCAACCATTACGGGCTGGGGCGCGAGGCATTGCGGGGGGGGCTGGCGGCGCTGGGCAGCATCGATGTGCTGAAGGTGGCGGCGATGCCGTTGTCGCAGTTGCGAGATGAGCTCAACGAAAgctg                                   <  6:65168/148‑1 (MQ=255)
                                                                                                    ttttGCCGAACAGGATGCGGCCACCAACCATTACGGGCTGGGGCGCGAGGCATTGCGGGTGGGGCTGGCGGCGCTGGGCAGCATCGATGTGCTGAAGGTggcggc                                                                             <  3:249237/105‑1 (MQ=255)
                                                                                                    ttttGCCGAACAGGATGCGGCCACCAACCATTACGGGCTGGGGCGCGAGGCATTGCGGGTGGGGCTGGCGGCGCTGGGCAGCATCGATGTGCTGAAGGTggcggc                                                                             >  4:249237/1‑105 (MQ=255)
                                                                                                         ccGAACAGGATGCGGCCACCAACCATTACGGGCTGGGGCGCGAGGCATTGCGGGTGGGGCTGGCGGCGCTGGGCAGCATCGATGTGCTGAAGGTGGCGGCGATGACGTTGTAGCAGTTGCGAGATGATATCAACGAAAGCTGCTTCATc                            >  3:38250/1‑149 (MQ=255)
                                                                                                          cGAACAGGATGCGGCCACCAACCATTACGGGCTGGGGCGCGAGGCATTGCGGGTGGGGCTGGCGGCGCTGGGCAGCATCGATGTGCTGAAGGTGGCGGCGATGCCGTTGTCGCAGTTGCGAGATGAGCTCAACGAAAGCTGCTTCATCg                           >  2:247995/1‑149 (MQ=255)
                                                                                                            aaCAGGATGCGGCCACCAACCATTACGGGCTGGGGCGCGAGGCATTGCGGGTGGGGCTGGCGGCGCTGGGCAGCATCGATGTGCTGAAGGTGGCGGCGATGCCGTTGTCGCAGTTGCGAGATGAGCTCAACGAAAGCTGCTTCATCGcc                         <  1:247995/149‑1 (MQ=255)
                                                                                                                 gATGCGGCCACCAACCATTACGGGCTGGGGCGCGAGGCATTGCGGGTGGGGCTGGCGGCGCTGGGCAGCATCGATGTGCTGAAGGTGGCGGCGATGCCGTTGTCGCAGTTGCGAGATGa                                                  >  3:219811/1‑119 (MQ=255)
                                                                                                                 gATGCGGCCACCAACCATTACGGGCTGGGGCGCGAGGCATTGCGGGTGGGGCTGGCGGCGCTGGGCAGCATCGATGTGCTGAAGGTGGCGGCGATGCCGTTGTCGCAGTTGCGAGATGa                                                  <  4:219811/119‑1 (MQ=255)
                                                                                                                 gATGCGGCCACCAACCATTACGGGCTGGGGCGCGAGGCATTGCGGGTGGGGCTGGCGGCGCTGGGCAGCATCGATGTGCTGAAGGTGGCGGCGATGCCGTTGTCGCAGTTGCGAGATGAGCTCAACGAAAGCTGCTTCATCGCCg                        <  2:104697/145‑1 (MQ=255)
                                                                                                                 gATGCGGCCACCAACCATTACGGGCTGGGGCGCGAGGCATTGCGGGTGGGGCTGGCGGCGCTGGGCAGCATCGATGTGCTGAAGGTGGCGGCGATGCCGTTGTCGCAGTTGCGAGATGAGCTCAACGAAAGCTGCTTCATCGCCg                        >  1:104697/1‑145 (MQ=255)
                                                                                                                 gATGCGGCCACCAACCATTACGGGCTGGGGCGCGAGGCATTGCGGGTGGGGCTGGCGGCGCTGGGCAGCATCGATGTGCTGAAGGTGGCGGCGATGCCGTTGTCGCAGTTGCGAGATGAGCTCAACGAAAGCTGCTTCATCGCCGtgtg                    <  4:131587/149‑1 (MQ=255)
                                                                                                                   tGCGGCCACCAACCATTACGGGCTGGGGCGCGAGGCATTGCGGGTGGGGCTGGCGGCGCTGGGCAGCATCGATGTGCTGAAGGTGGCGGCGATGCCGTTGTCGCAGTTGCGAGATGAGCTCAACGAAAGCTGCTTCATCGCCGTGTggg                  <  8:159331/149‑1 (MQ=255)
                                                                                                                      ggCCACCAACCATTACGGGCTGGGGCGCGAGGCATTGCGGGTGGGGCTGGCGGCGCTGGGCAGCATCGATGTGCTGAAGGTGGCGGCGATGCCGTTGTCGCAGTTGCGAGATGAGCTCAACGAAAGCTGCTTCATCGCCGTGTGGGGc                >  4:103554/1‑148 (MQ=255)
                                                                                                                                 aTTACGGGCTGGGGCGCGAGGCATTGCGGGTGGGGCTGGCGGCGCTGGGCAGCATCGATGTGCTGAAGGTGGCGGCGATGCCGTTGTCGCAGTTGCGAGATGAGCTCAACGAAAGCTGCTTCATCGCCGTGTGGGGCAACCAGGGGGc     >  7:179342/1‑148 (MQ=255)
                                                                                                                                  ttACGGGCTGGGGCGCGAGGCATTGCGGGTGGGGCTGGCGGCGCTGGGCAGCATCGATGTGCTGAAGGTGGCGGCGATGCCGTTGTCGCAGTTGCGAGATGAGCTCAACGAAAg                                      <  2:231946/114‑1 (MQ=255)
                                                                                                                                  ttACGGGCTGGGGCGCGAGGCATTGCGGGTGGGGCTGGCGGCGCTGGGCAGCATCGATGTGCTGAAGGTGGCGGCGATGCCGTTGTCGCAGTTGCGAGATGAGCTCAACGAAAg                                      >  1:231946/1‑114 (MQ=255)
                                                                                                                                    aCGGGCTGGGGCGCGAGGCATTGCGGGTGGGGCTGGCGGCGCTGGGCAGCATCGATGTGCTGAAGGTGGCGGCGATGCCGTTGTCGCAGTTGCGAGATGAGCTCAACGAAAGCTGCTTCATCGCCGTGTGGGGCAACCAGGGGGCCAc  <  3:157244/148‑1 (MQ=255)
                                                                                                                                             |                                                                                                                                          
CAGAACTCTCACCCTCGACGTCACTATCACGCCTGGCCGAGCACGTGCAGATGCCTGCGAGCAAAGTGCATCGCTACCTGCAGGCGCTGATTGCCAGTGGTTTTGCCGAACAGGATGCGGCCACCAACCATTACGGGCTGGGGCGCGAGGCATTGCGGGTGGGGCTGGCGGCGCTGGGCAGCATCGATGTGCTGAAGGTGGCGGCGATGCCGTTGTCGCAGTTGCGAGATGAGCTCAACGAAAGCTGCTTCATCGCCGTGTGGGGCAACCAGGGGGCCAC  >  NC_002947/5244679‑5244958

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑