Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,313,276 C→G 9.7% intergenic (‑595/+119) PP_16SG ← / ← msrQ 16S ribosomal RNA/methionine sulfoxide reductase heme‑binding subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,313,2760CG 9.7% 135.7 / 7.2 52intergenic (‑595/+119)PP_16SG/msrQ16S ribosomal RNA/methionine sulfoxide reductase heme‑binding subunit
Reads supporting (aligned to +/- strand):  ref base C (21/25);  new base G (3/2);  total (25/27)
Fisher's exact test for biased strand distribution p-value = 6.56e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.72e-01

TCGCTGGAAGTGGGGCGCATTATAAGGGGATTCGAGAGGGCGTCAACCTTTAATTTCAAGAAAGTGAAATATAAGGGGAAATACCGTATCGGGGCTGCCAAGTGTTCGCCACAACAGTTTCAGCGCCTATGAGATCGAGCGCCGCCCGCGCGGCGCTCGATCTCACAGACGCCAGAGAAACAAAGGCGGGCCCTCTCCACAGCCATTGCTATCTCAAGACAGGCCCTTGGCAGCCCCACCCCACTCTCAAACCGCCCTACCCTGCTACCTGGCAACCTTGGGAAGCGCCC  >  NC_002947/5313131‑5313420
                                                                                                                                                 |                                                                                                                                                
tCGCTGGTAGTGGGGCGCATTATAAGGGGATTCGAGAGGGCGTCAACCTTTAATTTCAAGAAAGTGAAATATAAGGGGAAATACCGTATCGGGGCTGCCAAGTGTTCGCCACAACAGTTTCAGCGCCTATGAGATCGAGCGccgcgcg                                                                                                                                                <  4:31088/148‑1 (MQ=255)
     ggAAGTGGGGCGCATTATAAGGGGATTCGAGAGGGCGTCAACCTTTAATTTCAAGAAAGTTAAATATAAGGGGAAATACCGTATCGGGGCTGCCAAGTGTTCGCCACAACAGTTTCAGCGCCTATGAGATCGAGCGCCGCGCGggcggc                                                                                                                                          >  4:307923/1‑149 (MQ=255)
       aaGTGGGGCGCATTATAAGGGGATTCGAGAGGGCGTCAACCTTTAATTTCAAGAAAGTGAAATATAAGGGGAAATACCGTATCGGGGCTGCCAAGTGTTCGCCACAACAGTTTCAGCGCCTATGAGATCGAGCGCCGCCCGCGCGgcgc                                                                                                                                        >  4:145862/1‑149 (MQ=255)
        aGTGGGCCTCATTATAATGGGATGCGAGAGGGCGTCAACCTTTAATTTCAAGAAAGTGAAATATAAGGGGAAATACCGTATCGGGGCGGCCAAGTGTTCGCCACAACAGTTTCAGCGCCTAGGAGATCGAGCGCCGCCCGCGCGGCGCt                                                                                                                                       <  3:252953/149‑1 (MQ=14)
          tGGGGCGCATTATAAGGGGATTCGAGAGGGCGTCAACCTTTAATTTCAAGAAAGTGAAATATAAGGGGAAATACCGTATCGGGGCTGCCAAGTGTTCGCCACAACAGTTTCAGCGCCTATGAGATCGAGCGCCGCCCGCGCGGCGCTCg                                                                                                                                     <  6:269436/149‑1 (MQ=255)
            gggCGCATTATAAGGGGATTCGAGAGGGCGTCAACCTTTAATTTCAAGAAAGTGAAATATAAGGGGAAATACCGTATCGGGGCTGCCAAGTGTTCGCCACAACAGTTTCAGCGCCTATGAGATCGAGCGCCGCGCGGGCGGCGCTCGa                                                                                                                                    <  3:307923/148‑1 (MQ=255)
            gggCGCATTATAAGGGGATTCGAGAGGGCGTCAACCTTTAATTTCAAGAAAGTGAAATATAAGGGGAAATACCGTATCGGGGCTGCCAAGTGTTCGCCACAACAGTTTCAGCGCCTATGAGATCGAGCGCCGCCCGCGCGGCGCTCGAt                                                                                                                                   >  6:175998/1‑149 (MQ=255)
            gggCCCATTATAAGGGGATTCGAGAGGGCGTCAACCTTTAATTTCAAGAAAGTGAAATATAAGGGGAAATACCGTATCGGGGCTGCCAAGTGTTCGCCACAACAGTTTCAGCGCCTATGAGATCGAGCGCCGCGCGGGCGGCGCTCGAt                                                                                                                                   <  5:114213/149‑1 (MQ=255)
             ggCGCATTATAAGGGGATTCGAGAGGGCGTCAACCTTTAATTTCAAGAAAGTGAAATATAAGGGGAAATACCGTATCGGGGCTGCCAAGTGTTCGCCACAACAGTTTCAGCGCCTATGAGATCGAGCGCCGCCCGCGCGGCGCTCGAtc                                                                                                                                  <  1:1486/149‑1 (MQ=255)
             ggCGCATTATAAGGGGATTCGAGAGGGCGTCAACCTTTAATTTCAAGAAAGTGAAATATAAGGGGAAATACCGTATCGGGGCTGCCAAGTGTTCGCCACAACAGTTTCAGCGCCTATGAGATCGAGCGCCGCCCGCGCGGCGCTCGAtc                                                                                                                                  <  4:321464/149‑1 (MQ=255)
             ggCGCATTATAAGGGGATTCGAGAGGGCGTCAACCTTTAATTTCAAGAAAGTGAAATATAAGGGGAAATACCGTATCGGGGCTGCCAAGTGTTCGCCACAACAGTTTCAGCGCCTATGAGATCGAGCGCCGCCCGCGCGGCGCTCGAtc                                                                                                                                  <  2:319663/149‑1 (MQ=255)
              gcgcATTATAAGGGGATTCGAGAGGGCGTCAACCTTTAATTTCAAGAAAGTGAAATATAAGGGGAAATACCGTATCGGGGCTGCCAAGTGTTCGCCACAACAGTTTCAGCGCCTATGAGATCGAGCGCCGCCCGCGCGGCGCTCGAtct                                                                                                                                 >  2:156162/1‑149 (MQ=255)
                  aTTATAAGGGGATTCGAGAGGGCGTCAACCTTTAATTTCAAGAAAGTGAAATATAAGGGGAAATACCGTATCGGGGCTGCCAAGTGTTCGCCACAACAGTTTCAGCGCCTATGAGATCGAGCGCCGCCCGCGCGGCGCTCGATCTcaca                                                                                                                             <  1:156162/149‑1 (MQ=255)
                       aaGGGGATTCGAGAGGGCGTCAACCTTTAATTTCAAGAAAGTGAAATATAAGGGGAAATACCGTATCGGGGCTGCCAAGTGTTCGCCACAACAGTTTCAGCGCCTATGAGATCGAGCGCCGCCCGCGCGGCGCTCGATCTCACAGACGc                                                                                                                        >  8:295089/1‑149 (MQ=255)
                          gggATTCGAGAGGGCGTCAACCTTTAATTTCAAGAAAGTGAAATATAAGGGGAAATACCGTATCGGGGCTGCCAAGTGTTCGCCACAACAGTTTCAGCGCCTATGAGATCGAGCGCCGCCCGCGCGGCGCTCGATCTCACAGACGc                                                                                                                        >  1:134558/1‑146 (MQ=255)
                          gggATTCGAGAGGGCGTCAACCTTTAATTTCAAGAAAGTGAAATATAAGGGGAAATACCGTATCGGGGCTGCCAAGTGTTCGCCACAACAGTTTCAGCGCCTATGAGATCGAGCGCCGCCCGCGCGGCGCTCGATCTCACAGACGc                                                                                                                        >  3:316911/1‑146 (MQ=255)
                           ttaTTCGAGATGGCGTAAACCTTTAATTTCAAGAAAGTGAAATATAAGGGGAAATACCGTATCGGGGCTGCCAAGTGTTCGCCACAACAGTTTCAGCGCCTATGAGATCGAGCGCCGCCCGCGCGGCGCTCGATCTCACAGACGCCaga                                                                                                                    <  4:53755/147‑1 (MQ=255)
                           ggATTCGAGAGGGCGTCAACCTTTAATTTCAAGAAAGTGAAATATAAGGGGAAATACCGTATCGGGGCTGCCAAGTGTTCGCCACAACAGTTTCAGCGCCTATGAGATCGAGCGCCGCCCGCGCGGCGCTCGATCTCACAGACGCCaga                                                                                                                    <  3:145862/149‑1 (MQ=255)
                            atctctagagGGTGTCAATCTTTAATTTAAATATATTTAAATATATGGGGAAATACGGTATGGGGGCTGCAAAGTGTTCGCCACAACATTTTCATGGCTTATTTGATCGAGGGCCGCCGGCGGGGCGGTCGATCTCACAGACGCCaga                                                                                                                    <  2:175205/142‑1 (MQ=255)
                                 gagaGGGCGTCAACCTTTAATTTCAAGAAAGTGAAATATAAGGGGAAATACCGTATCGGGGCTGCCAAGAGTTCGAAACAACAGTTTCAGCGCCTATGAGATAGAGCGCCGCACGCGCGGCGATCGATCTCAAAGACGCCaga                                                                                                                    >  1:175205/1‑143 (MQ=17)
                                        cGTCAACCTTTAATTTCAAGAAAGTGAAATATAAGGGGAAATACCGTATCGGGGCTGCCAAGTGTTCGCCACAACAGTTTCAGCGCCTATGAGATCGAGCGCCGCCCGCGCGGCGCTCGATCTCACAGACGCCAGAGAAACAAAGGCgg                                                                                                       <  5:175998/149‑1 (MQ=255)
                                         gTCAACCTTTAATTTCAAGAAAGTGAAATATAAGGGGAAATACCGTATCGGGGCTGCCAAGTGTTCGCCACAACAGTTTCAGCGCCTATGAGATCGAGCGCCGCCCGCGCGGCGCTCGATCTCACAGACGCCAGAGAAACAAAGGCggg                                                                                                      >  1:204164/1‑149 (MQ=255)
                                                   aaTTTCAAGAAAGTGAAATATAAGGGGAAATACCGTATCGGGGCTGCCAAGTGTTCGCCACAACAGTTTCAGCGCCTATGAGATCGAGCGCCGCCCGCGCGGCGCTCGATCTCACAGACGCCAGAGAAACAAAGGCGGGCCCTCTCca                                                                                             <  4:110007/148‑1 (MQ=255)
                                                   aaTTTCAAGAAAGTGAAATATAAGGGGAAATACCGTATCGGGGCTGCCAAGTGTTCGCCACAACAGTTTCAGCGCCTATGAGATCGAGCGCCGCCCGCGCGGCGCTCGATCTCACAGACGCCAGAGAAACAAAGGCGGGCCCTCTCca                                                                                             >  4:130881/1‑148 (MQ=255)
                                                    aTTTCAAGAAAGTGAAATATAAGGGGAAATACCGTATCGGGGCTGCCAAGTGTTCGCCACAACAGTTTCAGCGCCTATGAGATCGAGCGCCGCCCGCGCGGCGCTCGATCTCACAGACGCCAGAGAAACAAAGGCGGGCCCTCTCca                                                                                             >  2:325092/1‑147 (MQ=255)
                                                       tCAAGAAAGTGAAATATAAGGGGAAATACCGTATCGGGGCTGCCAAGTGTTCGCCACAACAGTTTCAGCGCCTATGAGATCGAGCGCCGCCCGCGCGGCGCTCGATCTCACAGACGCCaga                                                                                                                    >  5:65235/1‑121 (MQ=255)
                                                       tCAAGAAAGTGAAATATAAGGGGAAATACCGTATCGGGGCTGCCAAGTGTTCGCCACAACAGTTTCAGCGCCTATGAGATCGAGCGCCGCCCGCGCGGCGCTCGATCTCACAGACGCCaga                                                                                                                    <  6:65235/121‑1 (MQ=255)
                                                          aGAAAGTGAAATATAAGGGGAAATACCGTATCGGGGCTGCCAAGTGTTCGCCACAACAGTTTCAGCGCCTATGAGATCGAGCGCCGCCCGCGCGGCGCTCGATCTCACAGACGCCAGAGAAACAAAGGCGGGCCCTCTCCACAGCCAtt                                                                                     >  2:370251/1‑149 (MQ=255)
                                                            aaaGTGAAATATAAGGGGAAATACCGTATCGGGGCTGCCAAGTGTTCGCCACAACAGTTTCAGCGCCTATGAGATCGAGCGCCGCCCGCGCGGCGCTCGATCTCACAGACGCCAGAGAAACAAAGGCGGGCCCTCTCCACAGCCATTGc                                                                                   <  7:295089/149‑1 (MQ=255)
                                                             aaGTGAAATATAAGGGGAAATACCGTATCGGGGCTGCCAAGTGTTCGCCACAACAGTTTCAGCGCCTATGAGATCGAGCGCCGCCCGCGCGGCGCTCGATCTCACAGACGCCAGAGAAACAAAGGCGGGCCCTCTCCACAGCCATTGCt                                                                                  <  4:185159/149‑1 (MQ=255)
                                                               gTGAAATATAAGGGGAATTACCGTATCGGGGCTGCCATGGGTTCGCCACAACAGTTGCAGCGCCTATGAGATCGAGCGCCGCCCGCGCGGCGCTCGATCTCACTGACGCCAGAGAAACAAAGGCGGGCCCTCTCCACAGCCATTGCTAt                                                                                <  2:91659/149‑1 (MQ=25)
                                                               gTGAAATATAAGGGGAAATACCGTATCGGGGCTGCCAAGTGTTCGCCACAACAGTTTCAGCGCCTATGAGATCGAGCGCCGCCCGCGCGGCGCTCGATCTCACAGACGCCAGAGAAACAAAGGCGGGCCCTCTCCACAGCCATTGCTAt                                                                                <  3:339279/149‑1 (MQ=255)
                                                                tGAAATATAAGGGGAAATACCGTATCGGGGCTGCCAAGTGTTCGCCACAACAGTTTCAGCGCCTATGAGATCGAGCGCCGCCCGCGCGGCGCTCGATCTCACAGACGCCAGAGAAACAAAGGCGGGCCCTCTCCACAGCCATTGCTAtc                                                                               >  6:35897/1‑149 (MQ=255)
                                                                tGAAATATAAGGGGAAATACCGTATCGGGGCTGCCAAGTGTTCGCCACAACAGTTTCAGCGCCTATGAGATCGAGCGCCGCCCGCGCGGCGCTCGATCTCACAGACGCCAGAGAAACAAAGGCGGGCCCTCTCCACAGCCATTGCTAtc                                                                               >  6:41353/1‑149 (MQ=255)
                                                                 gAAATATAAGGGGAAATACCGTATCGGGGCTGCCAAGTGTTCGCCACAACAGTTTCAGCGCCTATGAGATCGAGCGCCGCCCGCGCGGCGCTCGATCTCACAGACGCCAGAGAAACAAAGGCGGGCCCTCTCCACAGCCATTGCTAtc                                                                               <  1:370251/148‑1 (MQ=255)
                                                                  aaaTATAAGGGGAAATACCGTATCGGGGCTGCCAAGTGTTCGCCACAACAGTTTCAGCGCCTATGAGATCGCGCGCCGCCCGCGCGGCGCTCGATCTCACAGACGCCAGAGAAACAAAGGAGGGCCCTCTCCACAGCCATGGCTAgct                                                                              >  6:301832/1‑148 (MQ=255)
                                                                       taAGGGGAAATACCGTATCGGGGCTGCCAAGTGTTCGCCACAACAGTTTCAGCGCCTATGAGATCGAGCGCCGCCCGCGCGGCGCTCGATCTCACAGACGCCAGAGAAACAAAGGCGGGCCCTCTCCACAGCCATTGCTATCTCAAGAc                                                                        <  1:325092/149‑1 (MQ=255)
                                                                                    cGTATCGGGGCTGCCAAGTGTTCGCCACAACAGTTTCAGCGCCTATGAGATCGAGCGCCGCCCGCGCGGCGCTCGATCTCACAGACGCCAGAGAAACAAAGGCGGGCCCTCTCCACAGCCATTGCTATCTCAAGACAGGCCCTTGGCAg                                                           <  4:316911/149‑1 (MQ=255)
                                                                                                     gtgtTCGCCACAACAGTTTCAGCGCCTATGAGATCGAGCGCCGCCCGCGCGGCGCTCGATCTCACAGACGCCAGAGAAACAAAGGCGGGCCCTCTCCACAGCCATTGCTATCTCAAGACAGGCCCTTGGCAGCCCCACCCCACTCTCa                                           >  7:256918/1‑148 (MQ=255)
                                                                                                       gtTCTCCAAAACAGTTTCAGCGCCTATTATATCGAGCGCCGCCCGCGCGGCGCTAGATCTCACAGACTCCAGAGAAACAAAGGCGGGCCCTCTCCACAGCCATTGCTATCTCAAGACAGGCCCTTGGCAGCCCCACCCCACTCTCaaa                                         <  8:256918/148‑1 (MQ=39)
                                                                                                        ttCGCCACAACAGTTTCAGCGCCTATGAGATCGAGCGCCGCCCGCGCGGCGCTCGATCTCACAGACGCCAGAGAAACAAAGGCGGGCCCTCTCCACAGCCATTGCTATCTCAAGACAGGCCCTTGGCAGCCCCACCCCACTCTCAAAc                                        <  2:134558/148‑1 (MQ=255)
                                                                                                              acaacaGTTTCAGCGCCTATGAGATCGAGCGCCGCCCGCGCGGCGCTCGATCTCACAGACGCCAGAGAAACAAAGGCGGGCCCTCTCCACAGCCATTGCTATCTCAAGACAGGCCCTTGGCAGCCCCACCCCACTCTCAAACCGCCCTa                                 >  7:116354/1‑149 (MQ=255)
                                                                                                                    gTTTCAGCGCCTATGAGATCGAGCGCCGCCCGCGCGGCGCTCGATCTCACAGACGCCAGAGAAACAAAGGCGGGCCCTCTCCACAGCCATTGCTATCTCAAGACAGGCCCTTGGCAGCCCCACCCCACTCTCAAACCGCCCTACCCTGc                           <  5:35897/149‑1 (MQ=255)
                                                                                                                    gTTTCAGCGCCTATGAGATCGAGCGCCGCCCGCGCGGCGCTCGATCTCACAGACGCCAGAGAAACAAAGGCGGGCCCTCTCCACAGCCATTGCTATCTCAAGACAGGCCCTTGGCAGCCCCACCCCACTCTCAAACCGCCCTACCCTGc                           <  5:41353/149‑1 (MQ=255)
                                                                                                                                aTGAGATCGAGCGCCGCCCGCGCGGCGCTCGATCTCACAGACGCCAGAGAAACAAAGGCGGGCCCTCTCCACAGCCATTGCTATCTCAAGACAGGCCCTTGGCAGCCCCACCCCACTCTCAAACCGCCCTACCCTGCTACCTGGCAAcc               >  7:271704/1‑149 (MQ=255)
                                                                                                                                   agaTCGAGCGCCGCCCGCGCGGCGCTAGATCTCACAGACGCCAGAGAAACAAAGGCGGGCCCTCTCCACAGCCATTGCTATCTCAAGACAGGCCCTTGGCAGCCCCACCCCACTCTCAAACCGCCCTACCCTGCTACCTGGCAACCTTg            >  4:47479/1‑149 (MQ=255)
                                                                                                                                    gaTCGAGCGCCGCCCGCGCGGCGCTCGATCTCACAGACGCCAGAGAAACAAAGGCGGGCCCTCTCCACAGCCATTGCTATCTCAAGACAGGCCCTTGGCAGCCCCACCCCCCTCTCAACCCGCCCTACCCTGCTACCTGGCAACCGTgg           >  4:290977/1‑149 (MQ=255)
                                                                                                                                     aTCGAGCGCCGCCCGCGCGGCGCTCGATCTCACAGACGCCAGAGAAACAAAGGCGGGCCCTCTCCACAGCCATTGCTATCTCAAGACAGGCCCtt                                                                <  5:81604/95‑1 (MQ=255)
                                                                                                                                     aTCGAGCGCCGCCCGCGCGGCGCTCGATCTCACAGACGCCAGAGAAACAAAGGCGGGCCCTCTCCACAGCCATTGCTATCTCAAGACAGGCCCtt                                                                >  6:81604/1‑95 (MQ=255)
                                                                                                                                      tCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGACGCCAGAGAAACAAAGGCGGGCCCTCTCCACCGCCATTGCTATCTCAAGACAGGCCCTTGGCAGCCCCACCCCACGCTCAACCCGCCCTACCCTCCTACCTGGCAACCTTgcg          >  5:209124/1‑146 (MQ=39)
                                                                                                                                      tCGAGCGACCCCCGCGCGGCGCTCCATATAACACACGCCAGACAAACAAAGGCGGGACCTCTCCAAATCCATTGCTATCTCAAGACAGGCCCTTGGCAGCCCCACCCCCCTCTCAAACCGCCCAACCCTCCTACCTCGCaagcattgc          >  8:35592/1‑141 (MQ=255)
                                                                                                                                       cGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGACGCCAGAGAAACAAAGGCGGGCCCTCTCCACAGCCATTGCTATCTCAAGACAGGCCCTTGGCAGCCCCACCCCACTCTCAAACCGCCCTACCCTGCTACCTGGCAACCTTggg          >  4:140940/1‑147 (MQ=255)
                                                                                                                                        gAGCGCCGCCCGCGCGGCGCTCGATCTCACAGACGCCAGAGAAACAAAGGCGGGCCCTCTCCACAGCCATTGCTATCTCAAGACAGGCCCTTGGCAGCCCCACCCCACTCTCAAACCGCCCTACCCTGCTACCTGGCAACCTTGGGaa        <  2:204164/148‑1 (MQ=255)
                                                                                                                                             ccgcccgcGCGGCGCTCGATCTCACAGACGCCAGAGAAACAAAGGCGGGCCCTCTCCACAGCCATTGCTATCTCAAGACAGGCCCTTGGCAGCCCCACCCCACTCTCAAACCGCCCTACCCTGCTACCTGGCAACCTTGGGAAGCGccc  >  2:362144/1‑149 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
TCGCTGGAAGTGGGGCGCATTATAAGGGGATTCGAGAGGGCGTCAACCTTTAATTTCAAGAAAGTGAAATATAAGGGGAAATACCGTATCGGGGCTGCCAAGTGTTCGCCACAACAGTTTCAGCGCCTATGAGATCGAGCGCCGCCCGCGCGGCGCTCGATCTCACAGACGCCAGAGAAACAAAGGCGGGCCCTCTCCACAGCCATTGCTATCTCAAGACAGGCCCTTGGCAGCCCCACCCCACTCTCAAACCGCCCTACCCTGCTACCTGGCAACCTTGGGAAGCGCCC  >  NC_002947/5313131‑5313420

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑