| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 5,318,451 | G→C | 8.2% | A308G (GCG→GGG) | ilvI ← | acetohydroxybutanoate synthase/acetolactate synthase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 5,318,451 | 0 | G | C | 8.2% | 181.7 / 6.9 | 61 | A308G (GCG→GGG) | ilvI | acetohydroxybutanoate synthase/acetolactate synthase |
| Reads supporting (aligned to +/- strand): ref base G (29/27); new base C (3/2); total (32/29) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.10e-01 | |||||||||||
GTCAATCTGCTTCCACCAGGCATCCAGCGCCGCCTTGTCAGGCTGCTCGCCGATTTCCTTGAGGATGCCGAGCATTTCGCTGAGCACGCTGTCGACCGGGCCGACGATTGGCACGTCGGCCTTGATCATCTTCGAGATCGACG‑CCGGGTCGATGTCGATGTGAATGATCTTGGCGTTCGGGCAGAACTTGGCCGGGCCGTTTACAACGCGGTCGTCGAAACGCGCACCGACGGCAAAGATGACATCGGCATGATGCATGGCCATGTTGGCGGTGTAGCTGCCAT > NC_002947/5318309‑5318592 | gTCAATCTGCTTCCACCAGGCATCCAGCGCCGCCTTGTCAGGCTGCTCGCCGATTTCCTTGAGGATGCCGAGCATTTCGCTGAGCACGCTGTCGACCGGGCCGACGATTGGCACGTCGGCCTTGATCATCTTCGAGATCGACG‑CCGGgt > 2:321517/1‑149 (MQ=255) tCAATCTGCTTCCACCAGGCATCCAGCGCCGCCTTGTCAGGCTGCTCGCCGATTTCCTTGAGGATGCCGAGCATTTCGCTGAGCACGCTGTCGACCGGGCCGACGATTGGCACGTCGGCCTTGATCATCTTCGAGATCGACG‑CCGGgtc < 4:263209/149‑1 (MQ=255) aaTCTGCTTCCACCAGGCATCCAGCGCCGCCTTGTCAGGCTGCTCGCCGATTTCCTTGAGGATGCCGAGCATTTCGCTGAGCACGCTGTCGACCGGGCCGACGATTGGCACGTCGGCCTTGATCATCTTCGAGATCGACG‑CCGGgtcga > 2:75586/1‑149 (MQ=255) tCTGCTTCCACCAGGCATCCAGCGCCGCCTTGTCAGGCTGCTCGCCGATTTCCTTGAGGATGCCGAGCATTTCGCTGAGCACGCTGTCGACCGGGCCGACGATTGGCACGTCGGCCTTGATCATCTTCGAGATCGACG‑CCGGgtcgatg > 8:54817/1‑149 (MQ=255) cTTCCACCAGGCATCCAGCGCCGCCTTGTCAGGCTGCTCGCCGATTTCCTTGAGGATGCCGAGCATTTCGCTGAGCACGCTGTCGACCGGGCCGACGATTGGCACGTCGGCCTTGATCATCTTCGACATCGACC‑CGGCgtcgatctcg < 5:132884/148‑1 (MQ=255) ttCCACCAGGCATCCAGCGCCGCCTTGTCAGGCTGCTCGCCGATTTCCTTGAGGATGCCGAGCATTTCGCTGAGCACGCTGTCGACCGGGCCGACGATTGGCACGTCGGCCTTGATCATCTTCGAGATCGACG‑CCGGgtcgatgtcgat > 8:308469/1‑149 (MQ=255) ccaccaGGCCTCCAGCGCCGCCTAGTCAGGCTGCTCGCCGATTTCCTTGCGGATGCCGACCAGTTCGCTGAGCACGCTGTCGCCCGGGCCGACGATTGGCACGTCGGCCTTGACCATCATCGAGAGCGACG‑CCGGgtcgatgtcgat < 7:281138/147‑1 (MQ=255) caccaGGCATCCAGCGCCGCCTTGTCAGGCTGCTCGCCGATTTCCTTGAGGATGCCGAGGATTTCGCTGAGCACGCTGTCGACCGGGCCGACGATTGGCACGTCGGCCTTGATCATCTTCGAGATCGACG‑CCGGGTcgatgtcgatgt > 1:291451/1‑148 (MQ=255) caccaGGCATCCAGCGCCGCCTTGTCAGGCTGCTCGCCGATTTCCTTGAGGATGCCGAGCATTTCGCTGAGCACGCTGTCGACCGGGCCGACGATTGGCACGTCGGCCTTGATCATCTTCGAGATCGACG‑CCGGGTcgatgtcgatgt < 1:202670/148‑1 (MQ=255) accaGGCATCCAGCGCCGCCTTGTCAGGCTGCTCGCCGATTTCCTTGAGGATGCCGAGCATTTCGCTGAGCACGCTGTCGACCGGGCCGACGATTGGCACGTCGGCCTTGATCATCTTCGAGATCGACG‑CCGGGTcgatgtcgatgt < 4:200128/147‑1 (MQ=255) accaGGCATCCAGCGCCGCCTTGTCAGGCTGCTCGCCGATTTCCTTGAGGATGCCGAGCATTTCGCTGAGCACGCTGTCGACCGGGCCGACGATTGGCACGTCGGCCTTGATCATCTTCGAGATCGACG‑CCGGGTcgatgtcgatgt > 3:200128/1‑147 (MQ=255) ccaGGCATCCAGCGCCGCCTTGTCAGGCTGCTCGCCGATTTGCTTGAGGATGCCGAGCATTTCGCTGAGCACGCTGTCGACCGGGCCGACGATTGGCACGTCGGCCTTGATCATCTTCGATATCGACG‑CCGGGTCTATGTCGATGTGaa < 3:15000/149‑1 (MQ=255) aTCCAGCGCCGCCTTGTCAGGCTGCTCGCCGATTTCCTTGAGGATGCCGATCATTTCGCTGAGCACGCTGTCGACCGGGCCGACGATTGGCACGTCGGCCTTGATCATCTTCGAGATCGACGCCCGGGTCGATGTCGATGTGAATGATc < 7:54817/149‑1 (MQ=255) aTCCAGCGCCGCCTTGTCAGGCTGCTCGCCGATTTCCTTGAGGATGCCGAGCATTTCGCTGAGCACGCTGTCGACCGGGCCGACGATTGGCACGTCGGCCTTGATCATCTTCGAGATCGACG‑CCGGGTCGATGTCGATGTGAATGATCt > 3:36881/1‑149 (MQ=255) aTCCAGCGCCGCCTTGTCAGGCTGCTCGCCGATTTCCTTGAGGATGCCGAGCATTTCGCTGAGCACGCTGTCGACCGGGCCGACGATTGGCACGTCGGCCTTGATCATCTTCGACATCGACC‑CGGCgtcgatctcgaaga > 4:251697/1‑137 (MQ=255) aTCCAGCGCCGCCTTGTCAGGCTGCTCGCCGATTTCCTTGAGGATGCCGAGCATTTCGCTGAGCACGCTGTCGACCGGGCCGACGATTGGCACGTCGGCCTTGATCATCTTCGACATCGACC‑CGGCgtcgatctcgaaga < 3:251697/140‑4 (MQ=255) cAGCGCCGCCTTGTCAGGCTGCTCGCCGATTTCCTTGAGGATGCCGAGCATTTCGCTGAGCACGCTGTCGACCGGGCCGACGATTGGCACGTCGGCCTTGATCATCTTCGAGATCGACG‑CCGGGTCGATGTCGATGTGAATGATCTTg < 5:67535/148‑1 (MQ=255) gccTTGTCAGGCTGCTCGCCGATTTCCTTGAGGATGCCGAGCATTTCGCTGAGCACGCTGTCGGCCGGGCCGACGATTGGCACGTCGGCCTTGATCATCTTCGAGATCGACG‑CCGGGTCGATGTCGATGTGAATGATCTTGGCGTTCgg > 1:240924/1‑149 (MQ=255) ccTTGTCAGGCTGCTCGCCGATTTCCTTGAGGATGCCGAGCATTTCGCTGAGCACGCTGTCGACCGGGCCGACGATTGGCACGTCGGCCTTGATCATCTTCGAGATCGACG‑CCGGgtc < 3:276210/118‑1 (MQ=255) ccTTGTCAGGCTGCTCGCCGATTTCCTTGAGGATGCCGAGCATTTCGCTGAGCACGCTGTCGACCGGGCCGACGATTGGCACGTCGGCCTTGATCATCTTCGAGATCGACG‑CCGGgtc > 4:276210/1‑118 (MQ=255) ccTTGTCAGGCTGCTCGCCGATTTCCTTGAGGATGCCGAGCATTTCGCTGAGCACGCTGTCGACCGGGCCGACGATTGGCACGTCGGCCTTGATCATCTTCGAGATCGACG‑CCGGGTCGATGTCGATGTGAATGATCTTGGCGTTCggg > 6:110891/1‑149 (MQ=255) cTTGTCAGGCTGCTCGCCGATTTCCTTGAGGATGCCGAGCATTTCGCTGAGCACGCTGTCGACCGGGCCGACGATTGGCACGTCGGCCTTGATCATCTTCGAGATCGACG‑CCGGGTCGATGTCGATGTGAATGATCTTGGCGTTCGGGc < 4:36881/149‑1 (MQ=255) tCAGGCTGCTCGCCGATTTCCTTGAGGATGCCGAGCATTTCGCTGAGCACGCTGTCGGCCGGGCCGACGATTGGCACGTCGGCCTTGATCATCTTCGAGATCGACG‑CCGGGTCGATGTCGATGTGAATGATCTTGGCGTTCGGGCAGaa < 2:240924/149‑1 (MQ=255) ggCTGCTCGCCGATTTCCTTGAGGATGCCGAGCATTTCGCTGAGCACGCTGTCGACCGGGCCGACGATTGGCACGTCGGCCTTGATCATCTTCGAGATCGACG‑CCGGGTCGATGTCGATGTGAATGATCTTGGCGTTCGGGCAGAACtt < 1:321517/149‑1 (MQ=255) gctgctCGCCGATTTCCTTGAGGATGCCGAGCATTTCGCTGAGCACGCTGTCGACCGGGCCGACGATTGGCACGTCGGCCTTGATCATCTTCGAGATCGACG‑CCGGGTCGATGTCGATGTGAATGATCTTGGCGTTCGGGCAGAACTTg < 1:75586/149‑1 (MQ=255) tgctCGCCGATTTCCTTGAGGATGCCGAGCATTTCGCTGAGCACGCTGTCGACCGGGCCGACGATTGGCACGTCGGCCTTGATCATCTTCGAGATCGACG‑CCGGGTCGATGTCGATGTGAATGATCTTGGCGTTCGGGCAGAACTTggc > 5:115287/1‑149 (MQ=255) aTTTCCTTGAGGATGCCGAGCATTTCGCTGAGCACGCTGTCGACCGGGCCGACGATTGGCACGTCGGCCGTGATCATCTTCGAGATCGACG‑CCGGGTCGATGTCGATGTGAATGATCTTGGCGTTCGGGCAGAACTTGGCCGGGCCGtt < 4:309686/149‑1 (MQ=255) tttCCTTGAGGATGCCGAGCATTTCGCTGAGCACGCTGTCGACCGGGCCGACGATTGGCACGTCGGCCTTGATCATCTTCGAGATCGACG‑CCGGGTCGATGTCGATGGGAATGATCTTGGCGTTCGGGCAGAACTTGGCCGGGCCGtt > 6:255172/1‑148 (MQ=255) cTTGAGGATGCCGAGCATTTCGCTGAGCACGCTGTCGACCGGGCCGACGATTGGCACGTCGGCCTTGATCATCTTCGAGATCGACG‑CCGGGTCGATGTCGATGTGAATGATCTTGGCGTTCGGGCAGAACTTGGCCGGGCCGTTTACaa > 3:103941/1‑149 (MQ=255) ttGAGGATGCCGAGCATTTCGCTGAGCACGCTGTCGACCGGGCCGACGATTGGCACGTCGGCCTTGATCATCTTCGAGATCGACG‑CCGGGTCGATGTCGATGTGAATGATCTTGGCGTTCg > 3:162170/1‑121 (MQ=255) ttGAGGATGCCGAGCATTTCGCTGAGCACGCTGTCGACCGGGCCGACGATTGGCACGTCGGCCTTGATCATCTTCGAGATCGACG‑CCGGGTCGATGTCGATGTGAATGATCTTGGCGTTCg < 4:162170/121‑1 (MQ=255) aGGATGCCGAGCATTTCGCTGAGCACGCTGTCGACAGGGCCGACGATTGGCACGTCGGCCTTGATCATCTTCGAGATCGCCG‑CCGGGTCGAGGTCGATGGGAATGAGCTTGGCGGTCGGGGAGAACTTGGGCGGGCCGTGTACAAcgcg > 2:324365/1‑149 (MQ=255) ccGAGCATTTCGCTGAGCACGCTGTCGACCGGGCCGACGATTGGCACGTCGGCCTTGATCATCTTCGAGATCGACG‑CCGGGTCGATGTCGATGTGAATGATCTTGGCGTTCGGGCAGAACTTGGCCGGGCCGTTTACAACGCGgtcgtc < 7:161667/149‑1 (MQ=255) ccGAGCATTTCGCTGAGCACGCTGTCGACCGGGCCGACGATTGGCACGTCGGCCTTGATCATCTTCGAGATCGACG‑CCGGGTCGATGTCGATGTGAATGATCTTGGCGTTCGGGCAGAACTTGGCCGGGCCGTTTACAACGCGgtcgt < 5:110891/148‑1 (MQ=255) cGAGCATTTCGCTGAGCACGCTGTCGACCGGGCCGACGATTGGCACGTCGGCCTTGATCATCTTCGAGATCGACG‑CCGGGTCGATGTCGATGTGAATGATCTTGGCGTTCGGGCAGAACTTGGCCGGGCCGTTTACAACGCGgtcgtc > 2:314842/1‑148 (MQ=255) gAGCATTTCGCTGAGCACGCTGTCGACCGGGCCGACGATTGGCACGTCGGCCTTGATCATCTTCGAGATCGACG‑CCGGGTCGATGTCGATGTGAATGATCTTGGCGTTCGGGCAGAACTTGGCCGGGCCGTTTACAACGCGGtcgtcg > 1:246615/1‑148 (MQ=255) tttCGCTGAGCACGCTGTCGACCGGGCCGACGATTGGCACGTCGGCCTTGATCATCTTCGAGATCGACG‑CCGGGTCGATGTCGATGTGAATGATCTTGGCGTTCGGGCAGAACTTGGCCGGGCCGTTTACAACGCGGTCGTCGAAAcgc < 2:291451/149‑1 (MQ=255) aGCACGCTGTCGACCGGGCCGACGATTGGCACGTCGGCCTTGATCATCTTCGAGATCGACG‑CCGGGTCGATGTCGATGTGAATGATCTTGGCGTTCGGGCAGAACTTGGCCGGGCCGTTTACAACGCGGTCGTCGAAACGCGCACCGAc < 2:246615/149‑1 (MQ=255) gCTGTCGACCGGGCCGACTATTGGCACGTCGGCCTTGATCATCTTCGAGATCGACG‑CCGGGTCGATGTCGATGTGAATGATCTTg < 6:65729/85‑1 (MQ=255) gCTGTCGACCGGGCCGACGATTGGCACGTCGGCCTTGATCATCTTCGAGATCGACG‑CCGGGTCGATGTCGATGTGAATGATCTTg > 5:65729/1‑85 (MQ=255) gACCGGGCCGACGATTGGCACGTCGGCCTTGATCATCTTCGAGATCGACG‑CCGGGTCGATGTCGATGTGAATGATCTTGGCGTTCGGGCAGAACTTGGCCGGGCCGTTTACAACGCGGTCGTCGAAACGCGCACCGACGGCAAAGATGa > 2:124503/1‑149 (MQ=255) aCCGGGCCGACGATTGGCACGTCGGCCTTGATCATCTTCGAGATCGACG‑CCGGGTCGATGTCGATGTGAATGATCTTGGCGTTCGGGCAGAACTTGGCCGGGCCGTTTACAACGCGGTCGTCGAAACGCGCACCGACGGCAAAGATGa > 3:289560/1‑148 (MQ=255) ggCCGACGATTGGCACGTCGGCCTTGATCATCTTCGAGATCGACG‑CCGGGTCGATGTCGATGTGAATGATCTTGGCGTTCGGGCAGAACTTGGCCGGGCCGTTTACAACGCGGTCGTCGAAACGCGCACCGACGGCAAAGATGACATCg < 7:308469/149‑1 (MQ=255) cgacgaTTGGCACGTCGGCCTTGATCATCTTCGAGATCGACG‑CCGGGGCGATGTCGATGTGAATGATCTTGGCGTTCGGGCAGAACTTGGCCGGGCCGTTTACAACGCGGTCGTCGAAACGCGCACCGACGGCAAAGATGACATCGGCa < 1:124503/149‑1 (MQ=255) gTCGGCCTTGATCATCTTCGAGATCGACG‑CCGGGTCGATGTCGATGTGAATGATCTTGGCGTTCGGGCAGAACTTGGCCGGGCCGTTTACAACGCGGTCGTCGAAACGCGCACCGACGGCAAAGATGACATCGGCATGATGCATGGCCa > 7:309803/1‑149 (MQ=255) ggCCTTGATCATCTTCGAGATCGACG‑CCGGGTCGATGTCGATGTGAATGATCTTGGCGTTCGGGCAGAACTTGGCCGGGCCGTTTACAACGCGGTCGTCGAAACGCGCACCGACGGCAAAGATGACATCGGCATGATGCATGGCCATg < 4:324128/148‑1 (MQ=255) gCCTTGATCATCTTCGAGATCGACG‑CCGGGTCGATGTCGATGTGAATGATCTTGGCGTTCGGGCAGAACTTGGCCGGGCCGTTTACAACGCGGTCGTCGAAACGCGCACCGACGGCAAAGATGACATCGGCATGATGCATGGCCATGtt > 6:137243/1‑149 (MQ=255) ccTTGATCATCTTCGAGATCGACG‑CCGGGTCGATGTCGATGTGAATGATCTTGGCGTTCGGGCAGAACTTGGCCGGGCCGTTTACAACGCGGTCGTCGAAACGCGCACCGACGGCAAAGATGACATCg > 3:180892/1‑128 (MQ=255) ccTTGATCATCTTCGAGATCGACG‑CCGGGTCGATGTCGATGTGAATGATCTTGGCGTTCGGGCAGAACTTGGCCGGGCCGTTTACAACGCGGTCGTCGAAACGCGCACCGACGGCAAAGATGACATCg < 4:180892/128‑1 (MQ=255) cTTGATCATCTTCGAGATCGACG‑CCGGGTCGATGTCGATGTGAATGATCTTGGCGTTCGGGCAGAACTTGGCCGGGCCGTTTACAACGCGGTCGTCGAAACGCGCACCGACGGCAAAGATGACATCGGCATGATGCATGGCCATGTTgg < 5:137243/149‑1 (MQ=255) cTTGATCATCTTCGAGATCGACG‑CCGGGTCGATGTCGATGTGAATGATCTTGGCGTTCGGGCAGAACTTGGCCGGGCCGTTTACAACGCGGTCGTCGAAACGCGCACCGACGGCAAAGATGACATCGGCATGATGCATGGCCATGTTgg < 8:309803/149‑1 (MQ=255) gATCATCTTCGAGATCGACG‑CCGGGTCGATGTCGATGAGAATGATCTAGGCGTTCGCGCAGAACTTGGCCGGGCACTTTACAACGCGGTCGTCGAAACGCGCACCCACCGCAAAGATGACATCGGCATGATCCATGCACATGATGGCgg > 8:248921/1‑149 (MQ=255) atcatcTTCGAGATCGACG‑CCGCGTCGATGTCGATGTGAATGAACTTGGCGTTCGGGCACAACTTGGCCCGACCGTTTACAACCCGGTCGTCGAAACGCGCACCGACGGCAAAGATGACATCCGCATGATCCAAGGCCAAGTTGGCg > 8:27076/1‑147 (MQ=255) atcTTCGAGATCGACG‑CCGGGTCGATGTCGATGTGAATGATCTTGGCGTTCGGGCAGAACTTGGCCGGGCCGTTTACAACGCGGTCGTCGAAACGCGCACCGACGGCAAAGATGACATCGGCATGATGCATGGCCATGTTGGCGgtg > 7:69772/1‑147 (MQ=255) atcTTCGAGATCGACG‑CCGGGTCGATGTCGATGTGAATGATCTTGGCGTTCGGGCAGAACTTGGCCGGGCCGTTTACAACGCGGTCGTCGAAACGCGCACCGACGGCAAAGATGACATCGGCATGATGCATGGCCATGTTGGCGgtg > 2:99442/1‑147 (MQ=255) ttCGAGATCGACG‑CCGGGTCGATGTCGATGTGAATGATCTTGGCGTTCGGGCAGAACTTGGCCGGGCCGTTTACAACGCGgtcgt > 7:222486/1‑85 (MQ=255) ttCGAGATCGACG‑CCGGGTCGATGTCGATGTGAATGATCTTGGCGTTCGGGCAGAACTTGGCCGGGCCGTTTACAACGCGgtcgt < 8:222486/85‑1 (MQ=255) tCGACATCGACC‑CGGCGTCGATGTCGATGTGAATGATCTTGGCGTTCGGGCAGAACTTGGCCGGGCCGTTTACAACGCGGTCGTCGAAACGCGCACCGACGGCAAAGATGACATCGGCATGATGCATGGCCATGTTGGCGGTGTAGCTg > 4:219088/1‑149 (MQ=255) cGAGATCGACG‑CCGGGTCGATGTCGATGTGAATGATCTTGGCGTTCGGGCAGAACTTGGCCGGGCCGTTTACAACGCGGTCGTCGAAACGCGCACCGACGGCAAAGATGACATCGGCATGATGCATGGCCATGTTGGCGGTGTAGCTGc > 1:103387/1‑149 (MQ=255) acaTCGACC‑CGGCGTCGATGTCGATGTGAATGATCTTGGCGTTCGGGCAGAACTTGGCCGGGCCGTTTACAACGCGGTCGTCGAAACGCGCACCGACGGCAAAGATGACATCGGCATGATGCATGGCCATGTTGGCGGTGTAGCTGCca > 5:375511/3‑149 (MQ=255) gaTCGACG‑CCGGGTCGATGTCGATGTGAATGATCTTGGCGTTCGGGCAGAACTTGGCCGGGCCGTTTACAACGCGGTCGTCGAAACGCGCACCGACGGCAAAGATGACATCGGCATGATGCATGGCCATGTTGGCGGTGTAGCTGCcat < 6:115287/149‑1 (MQ=255) | GTCAATCTGCTTCCACCAGGCATCCAGCGCCGCCTTGTCAGGCTGCTCGCCGATTTCCTTGAGGATGCCGAGCATTTCGCTGAGCACGCTGTCGACCGGGCCGACGATTGGCACGTCGGCCTTGATCATCTTCGAGATCGACG‑CCGGGTCGATGTCGATGTGAATGATCTTGGCGTTCGGGCAGAACTTGGCCGGGCCGTTTACAACGCGGTCGTCGAAACGCGCACCGACGGCAAAGATGACATCGGCATGATGCATGGCCATGTTGGCGGTGTAGCTGCCAT > NC_002947/5318309‑5318592 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |