Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,346,570 C→T 8.7% intergenic (+33/‑9) acsA‑II → / → PP_4703 acetyl‑CoA synthetase/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,346,5700CT 8.7% 127.8 / 2.7 46intergenic (+33/‑9)acsA‑II/PP_4703acetyl‑CoA synthetase/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (25/17);  new base T (2/2);  total (27/19)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.82e-01

GCGTATACTGCGCAAAATCGCCTGCAATGAGCTGGAAAACCTGGGCGATACCTCGACCCTGGCCGACCCGAGCGTGGTACAGGGCCTGATCGACAAACGCCTCAACCAGTAACCGGCGGCCGCAAATCTGGCCGCCCTGTACAGCAGGTCGCCATGGAAGCCCTTCGTCGCCGTATCGAAACCCAGGTCATGAGCCTCACCGGGCTGGCCCTCGGCCAGCTCGACCTGGAATCGCCCAAGGGCGACCCCGGCCTGTTCGGCCCGCACAGTATTAGCTGGCGCGTCCATGG  >  NC_002947/5346426‑5346715
                                                                                                                                                |                                                                                                                                                 
gcgTATACTGCGCAAAATCGCCTGCAATGAGCTGGAAAACCTGGGCGATACCTCGACCCTGGCCGACCCGAGCGTGGTACAGGGCCTGATCGACAAACGCCTCAACCAGTAACCGGCGGCCGCAAATCTGGCCGCCCTGTACAGTAGGt                                                                                                                                               >  2:344721/1‑149 (MQ=255)
       cTGCGCAAAATCGCCTGCAATGAGCTGGAAAACCTGGGCGATACCTCGACCCTGGCCGACCCGAGCGTGGTACAGGGCCTGATCGACAAACGCCTCAACCAGTAACCGGCGGCCGCAAATCTGGCCGCCCTGTACAGCAGGTCGCCATg                                                                                                                                        <  5:132258/149‑1 (MQ=255)
               aaTCGCCTGCAATGAGCTGGAAAACCTGGGCGATACCTCGACCCTGGCCGACCCGAGCGTGGTACAGGGCCTGATCGACAAACGCCTCAACCAGTAACCGGCGGCCGCAAATCTGGCCGCCCTGTACAGCAGGTc                                                                                                                                              >  8:91510/1‑135 (MQ=255)
               aaTCGCCTGCAATGAGCTGGAAAACCTGGGCGATACCTCGACCCTGGCCGACCCGAGCGTGGTACAGGGCCTGATCGACAAACGCCTCAACCAGTAACCGGCGGCCGCAAATCTGGCCGCCCTGTACAGCAGGTc                                                                                                                                              <  7:91510/135‑1 (MQ=255)
                    ccTGCAATGAGCTGGAAAACCTGGGCGATACCTCGACCCTGGCCGACCCGAGCGTGGTACAGGGCCTGATCGACAAACGCCTCAACCAGTAACCGGCGGCCGCAAATCTGGCCGCCCTGTACAGCAGGTCGCCATGGAAGCCCTtcgtc                                                                                                                           >  8:152311/1‑149 (MQ=255)
                       gCAATGAGCTGGAAAACCTGGGCGATACCTCGACCCTGGCCGACCCGAGCGTGGTACAGGGCCTGATCGACAAACGCCTCAACCAGTAACCGGCGGCCGCAAATCTGGCCGCCCTGTACAGCAGGTCGCCATGGAAGCCCTTCGTCGcc                                                                                                                        >  4:253345/1‑149 (MQ=255)
                             aGCTGGAAAACCTGGGCGATACCTCGACCCTGGCCGACCCGAGCGTGGTACAGGGCCTGATCGACAAACGCCTCAACCAGTAACCGGCGGCCGCAAATCTGGCCGCCCTGTACAGCAGGTCGCCATGGAAGCCCTTCGTCGCCGTATc                                                                                                                   >  1:334698/1‑148 (MQ=255)
                             aGCTGGAAAACCTGGGCGATACCTCGACCCTGGCCGACCCGAGCGTGGTACAGGGCCTGATCGACAAACGCCTCAACCAGTAACCGGCGGCCGCAAATCTGGCCGCCCTGTACAGCAGGTCGCCATGGAAGCCCTTCGTCGCCGTATc                                                                                                                   >  1:340077/1‑148 (MQ=255)
                                tGGAAAACCTGGGCGATACCTCGACCCTGGCCGACCCGAGCGTGGTACAGGGCCTGATCGACAAACGCCTCAACCAGTAACCGGCGGCCGCAAATCTGGCCGCCCTGTACAGCAGGTCGCCATGGAAg                                                                                                                                    >  2:62288/1‑128 (MQ=255)
                                 ggAAAACCTGGGCGATACCTCGACCCTGGCCGACCCGAGCGTGGTACAGGGCCTGATCGACAAACGCCTCAACCAGTAACCGGCGGCCGCAAATCTGGCCGCCCTGTACAGCAGGTCGCCATGGAAGCCCTTCGTCGCCGTATCGAAAc                                                                                                              >  5:183213/1‑149 (MQ=255)
                                      aCCTGGGCGATACCTCGACCCTGGCCGACCCGAGCGTGGTACAGGGCCTGATCGACAAACGCCTCAACCAGTAACCGGCGGCCGCAAATCTGGCCGCCCTGTACAGCAGGTCGCCATGGAAGCCCTTCGTCGCCGTATCGAAACCCAgg                                                                                                         >  5:296634/1‑149 (MQ=255)
                                         tGGGCGATACCTCGACCCTGGCCGACCCGAGCGTGGTACAGGGCCTGATCGACAAACGCCTCAACCAGTAACCGGCGGCCGCAAATCTGGCCGCCCTGTACAGCAGGTCGCCATGGAAGCCCTTCGTCGcc                                                                                                                        <  8:330097/131‑1 (MQ=255)
                                         tGGGCGATACCTCGACCCTGGCCGACCCGAGCGTGGTACAGGGCCTGATCGACAAACGCCTCAACCAGTAACCGGCGGCCGCAAATCTGGCCGCCCTGTACAGCAGGTCGCCATGGAAGCCCTTCGTCGcc                                                                                                                        >  7:330097/1‑131 (MQ=255)
                                         tGGGCGATACCTCGACCCTGGCCGACCCGAGCGTGGTACAGGGCCTGATCGACAAACGCCTCAACCAGTAACCGGCGGCCGCAAATCTGGCCGCCCTGTACAGCAGGTCGCCATGGAAGCCCTTCGTCGCCGTATCGAAACCCAGGTCa                                                                                                      >  3:321071/1‑149 (MQ=255)
                                            gCGATACCTCGACCCTGGCCGACCCGAGCGTGGTACAGGGCCTGATCGACAAACGCGTCAACCAGTAACCGGCGGCCGCAAATCTGGCCGCCCTGTACAGCAGGTCGCCATGGAAGCCCTTCGTCGCCGTATCGAAACCCAGGTCATGa                                                                                                   <  7:197042/149‑1 (MQ=255)
                                              gATACCTCGACCCTGGCCGACCCGAGCGTGGTACAGGGCCTGATCGACAAACGCCTCAACCAGTAACCGGCGGCCGCAAATCTGGCCGCCCTGTACAGCAGGTCGCCATGGAAGCCCTTCGTCGCCGTATCGAAACCCAGGTCATGAGc                                                                                                 <  7:152311/149‑1 (MQ=255)
                                                    tCGACCCTGGCCGACCCGAGCGTGGTACAGGGCCTGATCGACAAACGCCTCAACCAGTAACCGGCGGCCGCAAATCTGGCCGCCCTGTACAGCAGGTCGCCATGGAAGCCCTTCGTCGCCGTATCGAAACCCAGGTCATGAGCCTCAcc                                                                                           >  6:340826/1‑149 (MQ=255)
                                                      gACCCTGGCCGACCCGAGCGTGGTACAGGGCCTGATCGACAAACGCCTCAACCAGTAACCGGCGGCCGCAAATCTGGCCGCCCTGTACAGCAGGTCGCCATGGAAGCCCTTCGTCGCCGTATCGAAACCCAGGTCATGAGCCTCACCgg                                                                                         >  3:45052/1‑149 (MQ=255)
                                                           tGGCCGACCCGAGCGTGGTACAGGGCCTGATCGACAAACGCCTCAACCAGTAACCGGCGGCCGCAAATCTGGCCGCCCTGTACAGCAGGTCGCCATGGAAGCCCTTCGTCGCCGTATCGAAACCCAGGTCATGAGCCTCACCGGGCTgg                                                                                    >  1:192074/1‑149 (MQ=255)
                                                                 aCCCGAGCGTGGTACAGGGCCTGATCGACAAACGCCTCAACCAGTAACCGGCGGCCGCAAATCTGGCCGCCCTGTACAGCAGGTCGCCATGGAAGCCCTTCGTCGCCGTATCGAAACCCAGGTCATGAGCCTCACCGGGCTGGCCCTCg                                                                              <  4:321071/149‑1 (MQ=255)
                                                                     gAGCGTGGTACAGGGCCTGATCGACAAACGCCTCAACCAGTAACCGGCGGCCGCAAATCTGGCCGCCCTGTACAGCAGGTCGCCATGGAAGCCCTTCGTCGCCGTATCGAAACCCAGGTc                                                                                                       <  3:75046/120‑1 (MQ=255)
                                                                     gAGCGTGGTACAGGGCCTGATCGACAAACGCCTCAACCAGTAACCGGCGGCCGCAAATCTGGCCGCCCTGTACAGCAGGTCGCCATGGAAGCCCTTCGTCGCCGTATCGAAACCCAGGTc                                                                                                       >  4:75046/1‑120 (MQ=255)
                                                                     gAGCGTGGTACAGGGCCTGATCGACAAACGCCTCAACCAGTAACCGGCGGCCGCAAATCTGGCCGCCCTGTACAGCAGGTCGCCATGGAAGCCCTTCGTCGCCGTATCGAAACCCAGGTCATGAGCCTCACCGGGCTGGCCCTCGGc                                                                            >  8:197561/1‑147 (MQ=255)
                                                                     gAGCGTGGTACAGGGCCTGATCGACAAACGCCTCAACCAGTAACCGGCGGCCGCAAATCTGGCCGCCCTGTACAGCAGGTCGCCATGGAAGCCCTTCGTCGCCGTATCGAAACCCAGGTCATGAGCCTCACCGGGCTGGCCCTCGGc                                                                            >  8:192227/1‑147 (MQ=255)
                                                                               cAGGGCCTGATCGACAAACGCCTCAACCAGTAACCGGCGGCCGCAAATCTGGCCGCCCTGTACAGCAGGTCGCCATGGAAGCCCTTCGTCGCCGTATCGAAACCCAGGTCATGAGCCTCACCGGGCTGGCCCTCGGCCAGCTCGACCt                                                                 <  2:340077/148‑1 (MQ=255)
                                                                               cAGGGCCTGATCGACAAACGCCTCAACCAGTAACCGGCGGCCGCAAATCTGGCCGCCCTGTACAGCAGGTCGCCATGGAAGCCCTTCGTCGCCGTATCGAAACCCAGGTCATGAGCCTCACCGGGCTGGCCCTCGGCCAGCTCGACCt                                                                 <  2:334698/148‑1 (MQ=255)
                                                                               cAGGGCCTGATCGACAAACGCCTCAACCAGTAACCGGCGGCCGCAAATCTGGCCGCCCTGTACAGCAGGTCGCCATGGAAGCCCTTCGTCGCCGTATCGAAACCCAGGTCATGAGCCTCACCGGGCTGGCCCTCGGCCAGCTCGACCTg                                                                <  7:197561/149‑1 (MQ=255)
                                                                               cAGGGCCTGATCGACAAACGCCTCAACCAGTAACCGGCGGCCGCAAATCTGGCCGCCCTGTACAGCAGGTCGCCATGGAAGCCCTTCGTCGCCGTATCGAAACCCAGGTCATGAGCCTCACCGGGCTGGCCCTCGGCCAGCTCGACCTg                                                                <  7:192227/149‑1 (MQ=255)
                                                                                 gggCCTGATCGACAAACGCCTCAACCAGTAACCGGCGGCCGCAAATCTGGCCGCCCTGTACAGCAGGTCGCCATGGAAGCCCTTCGTCGCCGTATCGAAACCCAGGTCATGAGCCTCACCGGGCTGGCCCTCGGCCAGCTCGACCTGGa                                                              <  4:45052/149‑1 (MQ=255)
                                                                                  ggCCTGATCGACAAACGCCTCAACCAGTAACCGGCGGCCGCAAATCTGGCCGCCCTGTACAGTAGGTCGCCATGGAAGCCCTTCGTCGCCGTATCGAAACCCAGGTCATGAGCCTCACCGGGCTGGCCCTCGGCCAGCTCGACCTGGaa                                                             >  2:325614/1‑149 (MQ=255)
                                                                                            aCAAACGCCTCAACCAGTAACCGGCGGCCGCAAATCTGGCCGCCCTGTACAGTAGGTCGCCATGGAAGCCCTTCGTCGCCGTATCGAAACCCAGGTCATGAGCCTCACCGGGCTGGCCCTCGGCCAGCTCGACCTGGAATCGCCCAAgg                                                   <  1:325614/149‑1 (MQ=255)
                                                                                                aCGCCTCAACCAGTAACCGGCGGCCGCAAATCTGGCCGCCCTGTACAGCAGGTCGCCATGGaa                                                                                                                                     <  1:144333/63‑1 (MQ=255)
                                                                                                aCGCCTCAACCAGTAACCGGCGGCCGCAAATCTGGCCGCCCTGTACAGCAGGTCGCCATGGaa                                                                                                                                     >  2:144333/1‑63 (MQ=255)
                                                                                                aCGCCTCAACCAGTAACCGGCGGCCGCAAATCTGGCCGCCCTGTACAGCAGGTCGCCATGGAAGCCCTTCGTCGCCGTATCGAAACCCAGGTCATGAGCCTCACCGGGCTGGCCCTCGGCCAGCTCGACCTGGAATCGCCCAAGGGCGa                                               <  3:253345/149‑1 (MQ=255)
                                                                                                 cGCCTCAACCAGTAACCGGCGGCCGCAAATCTGGCCGCCCTGTACAGCAGGTCGCCATGGAAGCCCTTCGTCGCCGTATCGAAACCCAGGTCATGAGCCTCACCGGGCTGGCCCTCGGCCAGCTCGACCTGGAATCGCCCAAGGGCGAc                                              <  5:340826/149‑1 (MQ=255)
                                                                                                  gCCTCAACCAGTAACCGGCGGCCGCAAATCTGGCCGCCCTGTACAGCAGGTCGCCATGGAAGCCCTTCGTCGCCGTATCGAAACCCAGGTCATGAGCCTCACCGGGCTGGCCCTCGGCCAGCTCGACCTGGAATCGCCCAAGGGctccc                                             >  3:233459/1‑145 (MQ=255)
                                                                                                     tCAACCAGTAACCGGCGGCCGCAAATCTGGCCGCCCTGTACAGCAGGTCGCCATGGAAGCCCTTCGTCGCCGTATCGAAACCCAGGTCATGAGCCTCACCGGGCTGGCCCTCGGCCAGCTCGACCTGGAATCGCCCAAGGGCGACCCCg                                          >  6:117016/1‑149 (MQ=255)
                                                                                                     tCAACCAGTAACCGGCGGCCGCAAATCTGGCCGCCCTGTACAGCAGGTCGCCATGGAAGCCCTTCGTCGCCGTATCGAAACCCAGGTCATGAGCCTCACCGGGCTGGCCCTCGGCCAGCTCGACCTGGAATCGCCCAAGGGCGACCCCg                                          >  2:33197/1‑149 (MQ=255)
                                                                                                         ccAGTAACCGGCGGCCGCAAATCTGGCCGCCCTGTACAGCAGGTCGCCATGGAAGCCCTTCGTCGCCGTATCGAAACCCAGGTCATGAGCCTCCCCGGGCTGGCCCTCGGCCAGCTCGACCTGGAATCGCCCAAGGGCGCCCCCGGCCt                                      >  6:249080/1‑149 (MQ=255)
                                                                                                                 cggcggCCGCAAATCTGGCCGCCCTGTACAGCAGGTCGCCATGGAAGCCCTTCGTCGCCGTATCGAAACCCAGGTCATGAGCCTCACCGGGCTGGCCCTCGGCCAGCTCGACCTGGAATCGCCCAAGGGCGACCCCGGCCTGTTCGGcc                              >  4:21772/1‑149 (MQ=255)
                                                                                                                       ccGCAAATCTCGCCGCCCTGTACAGCAGGTCGCCATGGAAGCCCTTCGTCGCCGTATCGAAACCCAGGTCATGAGCCTCACCGGGCTGGCCCTCGGCCAGCTCGACCTGGAATCGCCCAAGGGCGACCCCGGCCTGTTCGGCCCGcaca                        <  6:296634/149‑1 (MQ=255)
                                                                                                                         gCAAATCTGGCCGCCCTGTACAGCAGGTCGCGATGGAAGCCCTTCGTCGCCGTATCGAAACCCAGGTCATGAGCCTCACCGGGCTGGCCCTCGGCCAGCTCGACCTGGAATCGCCCAAGGGCGACCCCGGCCTGTTCGGCCCGCACAg                       >  1:227985/1‑148 (MQ=255)
                                                                                                                          cAAATCTGGCCGCCCTGTACAGTAGGTCGCCATGGAAGCCCTTCGTCGCCGTATCGAAACCCAGGTCATGAGCCTCACCGGGCTGGCCCTCGGCCAGCTCGACCTGGAATCGCCCAAGGGCGACCCCAGCCTGTTCGGCCCGCACAGTa                     <  1:344721/149‑1 (MQ=255)
                                                                                                                               cTGGCCGCCCTGTACAGCAGGTCGCCATGGTAGCCCTTCGTCGCCGTATCGAAACCCAGGTCATGAGCCTCACCGGGCTGGCCCTCGGCCAGCTCGACCTGGAATCGCCCAAGGGCGACCCCGGCCTGTTCGGCCCGCACAGTATTAGc                >  1:28726/1‑149 (MQ=255)
                                                                                                                                  gccgccCTGTACAGCAGGTCGCCATGGAAGCCCTTCGTCGCCGTATCGAAACCCAGGTCATGAGCCTCACCGGGCTGGCCCTCGGCCAGCTCGACCTGGAATCGCCCAAGGGCGACCCCGGCCTGTTCGGCCCGCACAGTATTAGCt               <  2:192074/147‑1 (MQ=255)
                                                                                                                                          gTACAGCAGGTCGCCATGGAAGCCCTTCGTCGCCGTATCGAAACCCAGGTCATGAGCCTCACCGGGCTGGCCCTCGGCCAGCTCGACCTGGAATCGCCCAAGGGCGACCCCGGCCTGTTCGGCCCGCACAGTATTAGCTGGCGCGTCCa     >  4:111607/1‑149 (MQ=255)
                                                                                                                                             cagcagGTCGCCATGGAAGCCCTTCGTCGCCGTATCGAAACCCAGGTCATGAGCCTCACCGGGCTGGCCCTCGGCCAGCTCGACCTGGAATCGCCCAAGGGCGACCCCGGCCTGTTCGGCCCGCACAGTATTAGCTGGCGCGTCCATgg  <  4:334789/149‑1 (MQ=255)
                                                                                                                                              agcagGTCGCCATGGAAGCCCTTCGTCGCCGTATCGAAACCCAGGTCATGAGCCTCACCGGGCTGGCCCTCGGCCAGCTCGACCTGGAATCGCCCAAGGGCGACCCCGGCCTGTTCGGCCCGCACAGTATTAGCTGGCGCGTCCATgg  <  5:64608/148‑1 (MQ=255)
                                                                                                                                              agcagGTCGCCATGGAAGCCCTTCGTCGCCGTATCGAAACCCAGGTCATGAGCCTCACCGGGCTGGCCCTCGGCCAGCTCGACCTGGAATCGCCCAAGGGCGACCCCGGCCTGTTCGGCCCGCACAGTATTAGCTGGCGCGTCCATgg  >  6:64608/1‑148 (MQ=255)
                                                                                                                                                |                                                                                                                                                 
GCGTATACTGCGCAAAATCGCCTGCAATGAGCTGGAAAACCTGGGCGATACCTCGACCCTGGCCGACCCGAGCGTGGTACAGGGCCTGATCGACAAACGCCTCAACCAGTAACCGGCGGCCGCAAATCTGGCCGCCCTGTACAGCAGGTCGCCATGGAAGCCCTTCGTCGCCGTATCGAAACCCAGGTCATGAGCCTCACCGGGCTGGCCCTCGGCCAGCTCGACCTGGAATCGCCCAAGGGCGACCCCGGCCTGTTCGGCCCGCACAGTATTAGCTGGCGCGTCCATGG  >  NC_002947/5346426‑5346715

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑