Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,358,231 G→A 6.2% A177V (GCC→GTC)  infB ← translation initiation factor IF‑2

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,358,2310GA 6.2% 197.0 / 2.2 64A177V (GCC→GTC) infBtranslation initiation factor IF‑2
Reads supporting (aligned to +/- strand):  ref base G (31/29);  new base A (2/2);  total (33/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.28e-01

GCACGCGGAGTCGGCGCCTTTTCCTTCTCCTTGACCTTGACCTTGATCGACACACGAGGCGCTTCGCCACGACGACGGTCGTCATCACGGGTACGGCTTTCGTTGCGACGGGTCTCGTCTTTCTTGCGCTCGGCAGCACGGGCTGCGGCGTCTTCAGAGGCCGGGGCGTCTGCGACTACCGGGGCCGGAGCAGCGACCGGAGCAGCGGCAGGAGCCGGCTCTGGCTTGGCAGCAGGCGCAGGGGCGGCGGCTGCGGAGCCGGCGGCCTGACGGCGAGCCTGCTCTTCG  >  NC_002947/5358084‑5358371
                                                                                                                                                   |                                                                                                                                            
gcaCGCGGAGTCGGCGCCTTTTCCTTCTCCTTGACCTTGACCTTGATCGACACACGAGGCGCTTCGCCACGACGACGGTCGTCATCACGGGTACGGCTTTCGTTGCGACGGGTCTCGTCTTTCTTGCGCTCGGCAGCACGGGCTgcggc                                                                                                                                             <  8:213436/149‑1 (MQ=255)
 caCGCGGAGTCGGCGCCTTTTCCTTCTCCTTGACCTTGACCTTGATCGACACACGAGGCGCTTCGCCACGACGACGGTCGTCATCACGGGTACGGCTTTCGTTGCGACGGGTCTCGTCTTTCTTGCGCTCGGCAGCACGGGCTgcggcg                                                                                                                                            >  3:120144/1‑149 (MQ=255)
   cgcgGAGTCGGCGCCTTTTCCTTCTCCTTGACCTTGACCTTGATCGACACACGAGGCGCTTCGCCACGACGACGGTCGTCATCACGGGTACGGCTTTCGTTGCGACGGGTCTCGTCTTTCTTGCGCTCGGCAGCACGGGCTGCGGCGTc                                                                                                                                          <  6:104232/149‑1 (MQ=255)
       gAGTCGGCGCCTTTTCCTTCTCCTTGACCTTGACCTTGATCGACACACGAGGCGCTTCGCCACGACGACGGTCGTCATCACGGGTACGGCTTTCGTTGCGACGGGTCTCGTCTTTCTTGCGCTCGGCAGCACGGGCTGCGGCGTCtt                                                                                                                                        >  6:111908/1‑147 (MQ=255)
             gcgcCTTTTCCTTCTCCTTGACCTTGACCTTGATCGACACACGAGGCGCTTCGCCACGACGACGGTCGTCATCACGGGTACGGCTTTCGTTGCGACGGGTCTCGTCTTTCTTGCGCTCGGCAGCACGGGCTGCGGCGTCTTCAGAGGcc                                                                                                                                <  6:23757/149‑1 (MQ=255)
                ccTTTTCCTTCTCCTTGACCTTGACCTTGATCGACACACGAGGCGCTTCGCCACGACGACGGTCGTCATCACGGGTACGGCTTTCGTTGCGACGGGTCTCGTCTTTCTTGCGCTCGGCAGCACGGGCTGCGGCGTCTTCAGAGGCCggg                                                                                                                             <  6:258369/149‑1 (MQ=255)
                 cTTTTCCTTCTCCTTGACCTTGACCTTGATCGACACACGAGGCGCTTCGCCACGACGACGGTCGTCATCACGGGTACGGCTTTCGTTGCGACGGGTCTCGTCTTTCTTGCGCTCGGCAGCACGGGCTGCGGCGTCTTCAGAGGCCgggg                                                                                                                            >  8:127381/1‑149 (MQ=255)
                    ttCCTTCTCCTTGACCTTGACCTTGATCGACACACGAGGCGCTTCGCCACGACGACGGTCGTCATCACGGGTACGGCTTTCGTTGCGACGGGTCTCGTCTTTCTTGCGCTCGGCAGCACGGGCTGCGGCCTCTGAAGACGCCgcagc                                                                                                                           <  4:358558/147‑5 (MQ=255)
                       cTTCTCCTTGACCTTGACCTTGATCGACACACGAGGCGCTTCGCCACGACGACGGTCGTCATCACGGGTACGGCTTTCGTTGCGACGGGTCTCGTCTTTCTTGCGCTCGGCAGCACGGGCTGCGGCGTCTTCAGAGGCCGGGGc                                                                                                                           >  1:220179/1‑144 (MQ=255)
                       cTTCTCCTTGACCTTGACCTTGATCGACACACGAGGCGCTTCGCCACGACGACGGTCGTCATCACGGGTACGGCTTTCGTTGCGACGGGTCTCGTCTTTCTTGCGCTCGGCAGCACGGGCTGCGGCGTCTTCAGAGGCCGGGGc                                                                                                                           <  2:220179/144‑1 (MQ=255)
                       cTTCTCCTTGACCTTGACCTTGATCGACACACGAGGCGCTTCGCCACGACGACGGTCGTCATCACGGGTACGGCTTTCGTTGCGACGGGTCTCGTCTTTCTTGCGCTCGGCAGCACGGGCTGCGGCGTCTTCAGAGGCCGGGGCGTCTg                                                                                                                      <  5:199177/149‑1 (MQ=255)
                        ttCTCCTTGACCTTGACCTTGATCGACACACGAGGCGCTTCGCCACGACGACGGTCGTCATCACGGGTACGGCTTTCGTTGCGACGGGTCTCGTCTTTCTTGCGCTCGGCAGCACGGGCTGCGGCGTCTTCaga                                                                                                                                    >  7:228240/1‑134 (MQ=255)
                        ttCTCCTTGACCTTGACCTTGATCGACACACGAGGCGCTTCGCCACGACGACGGTCGTCATCACGGGTACGGCTTTCGTTGCGACGGGTCTCGTCTTTCTTGCGCTCGGCAGCACGGGCTGCGGCGTCTTCaga                                                                                                                                    <  8:228240/134‑1 (MQ=255)
                          ctcCTTGACCTTGACCTTGATCGACACACGAGGCGCTTCGCCACGACGACGGTCGTCATCACGGGTACGGCTTTCGTTGCGACGGGTCTCGTCTTTCTTGCGCTCGGCAGCACGGGCTGCGGCGTCTTCAGAGGCCGGGGCGTCTGc                                                                                                                     <  4:42947/147‑1 (MQ=255)
                                  ccttgaccttgaTCGACACACGAGGCGCTTCGCCACGACGACGGTCGTCATCACGGGTACGGCTTTCGTTGCGACGGGTCTCGTCTTTCTTGCGCTCGGCAGCACGGGCTGCGGCGTCTTCAGAGGCCGGGGCGTCTGCGACTACCggg                                                                                                           <  2:369900/149‑1 (MQ=255)
                                   cttgaccttgaTCGACACACGAGGCGCTTCGCCACGACGACGGTCGTCATCACGGGTACGGCTTTCGTTGCGACGGGTCTCGTCTTTCTTGCGCTCGGCAGCACGGGCTGCGGCGTc                                                                                                                                          >  6:172811/1‑117 (MQ=255)
                                   cttgaccttgaTCGACACACGAGGCGCTTCGCCACGACGACGGTCGTCATCACGGGTACGGCTTTCGTTGCGACGGGTCTCGTCTTTCTTGCGCTCGGCAGCACGGGCTGCGGCGTc                                                                                                                                          <  5:172811/117‑1 (MQ=255)
                                      gaccttgaTCGACACACGAGGCGCTTCGCCACGACGACGGTCGTCATCACGGGTACGGCTTTCGTTGCGACGGGTCTCGTCTTTCTTGCGCTCGGCAGCACGGGCTGCGGCGTCTTCAGAGGCCGGGGCGTCTGCGACTACCGGGGcc                                                                                                        <  8:242255/148‑1 (MQ=255)
                                       accttgaTCGACACACGAGGCGCTTCGCCACGACGACGGTCGTCATCACGGGTACGGCTTTCGTTGCGACTGGTCTCGTCTTTCTTGCGCTCGGCAGCACGGGCTGCGGCGTCTTCAGAGGCCGGGGCGTCTGCGACTACCGGGGCCg                                                                                                       <  6:200394/148‑1 (MQ=255)
                                       accttgaTCGACACACGAGGCGCTTCGCCACGACGACGGTCGTCATCACGGGTACGGCTTTCGTTGCGACGGGTCTCGTCTTTCTTGCGCTCGGCAGCACGGGCTGCGGCGTCTTCAGAGGCCGGGGCGTCTGCGACTACCGGGGCCg                                                                                                       <  4:120144/148‑1 (MQ=255)
                                         cttgaTCGACACACGAGGCGCTTCGCCACGACGACGGTCGTCATCACGGGTACGGCTTTCGTTGCGACGGGTCTCGTCTTTCTTGCGCTCGGCAGCACGGGCTGCGGCGTCTTCAGAGGCCGGGGCGTCTGCGACTACCGGGGCCGGag                                                                                                    >  1:103430/1‑149 (MQ=255)
                                         cttgaTCGACACACGAGGCGCTTCGCCACGACGACGGTCGTCATCACGGGTACGGCTTTCGTTGCGACGGGTCTCGTCTTTCTTGCGCTCGGCAGCACGGGCTGCGGCGTCTTCAGAGGCCGGGGCGTCTGCGACTACCGGGGCCGGag                                                                                                    <  5:111908/149‑1 (MQ=255)
                                          ttgaTCGACACACGAGGCGCTTCGCCACGACGACGGTCGTCATCACGGGTACGGCTTTCGTTGCGACGGGTCTCGTCTTTCTTGCGCTCGGCAGCACGGGCTGCGGCGTCTTCAGAGGCCGGGGCGTCTGCGACTACCGGGGCCGGag                                                                                                    >  4:84508/1‑148 (MQ=255)
                                          tcgaTCGACACACGAGGCGCTTCGCCACGACGACGGTCGTCATCACGGGTACGGCTTTCGTTGCGACGGGTCTCGTCTTTCTTGCGCTCGGCAGCACGGGCTGCGGCGTCTTCAGAGGCCGGGGCGTCTGCGACTACCGGGGCCGGagc                                                                                                   >  3:78254/3‑149 (MQ=255)
                                             aTCGACACACGAGGCGCTTCGCCACGACGACGGTCGTCATCACGGGTACGGCTTTCGTTGCGACGGGTCTCGTCTTTCTTGCGCTCGGCAGCACGGGCTGCGGCGTCTTCAGAGGCCGGGGCGTCTGCGACTACCGGGGCCGGagcag                                                                                                 <  6:374106/148‑1 (MQ=255)
                                                   acacGAGGCGCTTCGCCACGACGACGGTCGTCATCACGGGTACGGCTTTCGTTGCGACGGGTCTCGTCTTTCTTGCGCTCGGCAGCACGGGCTGCGGCGTCTTCAGAGGCCGGGGCGTCTGCGACTACCGGGGCCGGAGCAGCGACCg                                                                                           >  4:51702/1‑148 (MQ=255)
                                                     acGAGGCGCTTCGCCACGACGACGGTCGTCATCACGGGTACGGCTTTCGTTGCGACGGGTCTCGTCTTTCTTGCGCTCGGCAGCACGGGCTGCGGCGTCTTCAGAGGCCGGGGCGTCTGCGACTACCGGGGCCgg                                                                                                      >  5:121118/1‑135 (MQ=255)
                                                     acGAGGCGCTTCGCCACGACGACGGTCGTCATCACGGGTACGGCTTTCGTTGCGACGGGTCTCGTCTTTCTTGCGCTCGGCAGCACGGGCTGCGGCGTCTTCAGAGGCCGGGGCGTCTGCGACTACCGGGGCCgg                                                                                                      <  6:121118/135‑1 (MQ=255)
                                                     acGAGGCGCTTCGCCACGACGACGGTCGTCATCACGGGTACGGCTTTCGTTGCGACGGGTCTCGTCTTTCTTGCGCTCGGCAGCACGGGCTGCGGCGTCTTCAGAGGCCGGGGCGTCTGCGACTACCGGGGCCGGAGCAGCGACCg                                                                                           >  2:329554/1‑146 (MQ=255)
                                                     acGAGGCGCTTCGCCACGACGACGGTCGTCATCACGGGTACGGCTTTCGTTGCGACGGGTCTCGTCTTTCTTGCGCTCGGCAGCACGGGCTGCGGCGTCTTCAGAGGCCGGGGCGTCTGCGACTACCGGGGCCGGAGCAGCGACCg                                                                                           <  1:329554/146‑1 (MQ=255)
                                                     acGAGGCGCTTCGCCACGACGACGGTCGTCATCACGGGTACGGCTTTCGTTGCGACGGGTCTCGTCTTTCTTGCGCTCGGCAGCACGGGCTGCGGCGTCTTCAGAGGCCGGGGCGTCTGCGACTACCGGGGCCGGAGCAGCGACCGGag                                                                                        >  7:72193/1‑149 (MQ=255)
                                                                 gCCACGACGACGGTCGTCATCACGGGTACGGCTTTCGTTGCGACGGGTCTCGTCTTTCTTGCGCTCGGCAGCACGGGCTGCGGCGTCTTCAGAGGCCggg                                                                                                                             <  5:76084/100‑1 (MQ=255)
                                                                 gCCACGACGACGGTCGTCATCACGGGTACGGCTTTCGTTGCGACGGGTCTCGTCTTTCTTGCGCTCGGCAGCACGGGCTGCGGCGTCTTCAGAGGCCggg                                                                                                                             >  6:76084/1‑100 (MQ=255)
                                                                 gCCACGACGACGGTCGTCATCACGGGTACGGCTTTCGTTGCGACGGGTCTCGTCTTTCTTGCGCTCGGCAGCACGGGCTGCGGCGTCTTCAGAGGCCGGGGCGTCTGCGACTACCGGGGCCGGAGCAGCGACCGGAGCAGCGGCAGGa                                                                             <  3:51702/148‑1 (MQ=255)
                                                                  ccACGACGACGGTCGTCATCACGGGTACGGCTTTCGTTGCGACGGGTCTCGTCTTTCTTGCGCTCGGCAGCACGGGCTGCGGCGTCTTCAGAGGCCGGGGCGTCTGCGACTACCGGGGCCGGAGCAGCGACCGGAGCAGCGGCAGGAGc                                                                           <  3:84508/149‑1 (MQ=255)
                                                                  ccACGACGACGGTCGTCATCACGGGTACGGCTTTCGTTGCGACGGGTCTCGTCTTTCTTGCGCTCGGCAGCACGGGCTGCGGCGTCTTCAGAGGCCGGGGCGTCTGCGACTACCGGGGCCGGAGCAGCGACCGGAGCAGCGGCAGGAGc                                                                           >  2:193328/1‑149 (MQ=255)
                                                                   cACGACGACGGTCGTCATCACGGGTACGGCTTTCGTTGCGACGGGTCTCGTCTTTCTTGCGCTCGGCAGCACGGGCTGCGGCGTCTTCAGAGGCCGGGGCGTCTGCGACTACCGGGGCCGGAGCAGCGACCGGAGCAGCGGCAGGAGcc                                                                          <  2:103430/149‑1 (MQ=255)
                                                                              tcgtcATCACGGGTACGGCTTTCGTTGCGACGGGTCTCGTCTTTCTTGCGCTCGGCAGCACGGGCTGCGACGTCTTCAGAGGCCGGGGCGTCTGCGACTACCGGGGCCGGAGCAGCGACCGGAGCAGCGGCAGGAGCCGGCTCTGGCtt                                                               >  2:175775/1‑149 (MQ=255)
                                                                              tcgtcATCACGGGTACGGCTTTCGTTGCGACGGGTCTCGTCTTTCTTGCGCTCGGCAGCACGGGCTGCGACGTCTTCAGAGGCCGGGGCGTCTGCGACTACCGGGGCCGGAGCAGCGACCGGAGCAGCGGCAGGAGCCGGCTCTGGCtt                                                               >  2:170149/1‑149 (MQ=255)
                                                                                    tcaCGGGTACGGCTTTCGTTGCGACGGGACTCGTCTTTCTTGCGCTCGGCAGCACGGGCTGCGGCGTCTTCAGAGGCCGGGGCGTCTGCGACTACCGGGGCCGGAGCAGCGACCGGAGCAGCGGCAGGAGCCGGCTCTGGCTTGgcag                                                          >  1:208034/1‑148 (MQ=255)
                                                                                       cGGGTACGGCTTTCGTTGCGACGGGTCTCGTCTTTCTTGCGCTCGGCAGCACGGGCTGCGGCGTCTTCAGAGGCCGGGGCGTCTGCGACTACCGGGGCCGGAGCAGCGACCGGAGCAGCGGCAGGAGCCGGCTCTGGCTTGGCAGCAgg                                                      >  4:84107/1‑149 (MQ=255)
                                                                                           tACGGCTTTCGTTGCGACGGGTCTCGTCTTTCTTGCGCTCGGCAGCACGGGCTGCGGCGTCTTCAGAGGCCGGGGCGTCTGCGACTACCGGGGCCGGAGCAGCGACCGGAGCAGCGGCAGGAGCCGGctc                                                                     <  4:235592/130‑1 (MQ=255)
                                                                                           tACGGCTTTCGTTGCGACGGGTCTCGTCTTTCTTGCGCTCGGCAGCACGGGCTGCGGCGTCTTCAGAGGCCGGGGCGTCTGCGACTACCGGGGCCGGAGCAGCGACCGGAGCAGCGGCAGGAGCCGGctc                                                                     >  3:235592/1‑130 (MQ=255)
                                                                                              ggCTTTCGTTGCGACGGGTCTCGTCTTTCTTGCGCTCGGCAGCACGGGCTGCGGCGTCTTCAGAGGCCGGGGCGTCTGCGACTACCGGGGCCGGAGCAGCGACCGGAGCAGCGGCAGGAGCCGGCTCTGGCTTGGCAGCAGGCGCAggg                                               >  4:45594/1‑149 (MQ=255)
                                                                                                cTTTCGTTGCGACGGGTCTCGTCTTTCTTGCGCTCGGCAGCACGGGCTGCGGCGTCTTCAGAGGCCGGGGCGTCTGCGACTACCGGGGCCGGAGCAGCGACCGGAGCAGCGGCAGGAGCCGGCTCTg                                                                   >  5:100641/1‑127 (MQ=255)
                                                                                                cTTTCGTTGCGACGGGTCTCGTCTTTCTTGCGCTCGGCAGCACGGGCTGCGGCGTCTTCAGAGGCCGGGGCGTCTGCGACTACCGGGGCCGGAGCAGCGACCGGAGCAGCGGCAGGAGCCGGCTCTg                                                                   <  6:100641/127‑1 (MQ=255)
                                                                                                         cGACGGGTCTCGTCTTTCTTGCGCTCGGCAGCACGGGCTGCGGCGTCTTCAGAGGCCGGGGCGTCTGCGTCTACCGGGGCCGGAGCAGCGACCGGAGCAGCGGCAGGAGCCGGCTCTGGCTTGGCAGCAGGCGCAGGGGCGGCGGCTGc                                    <  3:45594/149‑1 (MQ=255)
                                                                                                                tctcGTCTTTCTTGCGCTCGGCAGCACGGGCTGCGGCGTCTTCAGAGGCCGGGGCGTCTGCGACTACCGGGGCCGGAGCAGCGACCGGAGCAGCGGCAGGAGCCGGCTCTGGCTTGGCAGCAGGCGCAGGGGCGGCGGCTGCGGAGCcg                             >  6:216172/1‑149 (MQ=255)
                                                                                                                tctcGTCTTTCTTGCGCTCGGCAGCACGGGCTGCGGCGTCTTCAGAGGCCGGGGCGTCTGCGACTACCGGGGCCGGAGCAGCGACCGGAGCAGCGGCAGGAGCCGGCTCTGGCTTGGCAGCAGGCGCAGGGGCGGCGGCTGCGGAGCcg                             <  8:72193/149‑1 (MQ=255)
                                                                                                                  tcGTCTTTCTTGCGCTCGGCAGCACGGGCTGCGGCGTCTTCAGAGGCCGGGGCGTCTGCGACTACCGGGGCCGGAGCAGCGACCGGAGCAGCGGCAGGAGCCGGCTCTGGCTTGGcagcag                                                       >  2:312645/1‑121 (MQ=255)
                                                                                                                  tcGTCTTTCTTGCGCTCGGCAGCACGGGCTGCGGCGTCTTCAGAGGCCGGGGCGTCTGCGACTACCGGGGCCGGAGCAGCGACCGGAGCAGCGGCAGGAGCCGGCTCTGGCTTGGCAGCAGGCGCAGGGGCGGCGGCTGCGGAGCcggc                           >  6:101741/1‑149 (MQ=255)
                                                                                                                     tctttcttGCGCTCGGCAGCACGGGCTGCGGCGTCTTCAGAGGCCGGGGCGTCTGCGACTACCGGGGCCGGAGCAGCGACCGGAGCAGCGGCAGGAGCCGGCTCTGGCTTGGCAGCAGGCGCAGGGGCGGCGGCTGCGGAGCCggcggc                        >  8:8903/1‑149 (MQ=255)
                                                                                                                     tctttcttGCGCTCGGCAGCACGGGCTGCGGCGTCTTCAGAGGCCGGGGCGTCTGCGACTACCGGGGCCGGAGCAGCGACCGGAGCAGCGGCAGGAGCCGGCTCTGGCTTGGCAGCAGGCGCAGGGGCGGCGGCTGCGGAGCCggcggc                        >  3:225334/1‑149 (MQ=255)
                                                                                                                      ctttcttGCGCTCGGCAGCACGGGCTGCGGCGTCTTCAGAGGCCGGGGCGTCTGCGACTACCGGGGCCGGAGCAGCGACCGGAGCAGCGGCAGGAGCCGGCTCTGGCtt                                                               >  2:92934/1‑109 (MQ=255)
                                                                                                                      ctttcttGCGCTCGGCAGCACGGGCTGCGGCGTCTTCAGAGGCCGGGGCGTCTGCGACTACCGGGGCCGGAGCAGCGACCGGAGCAGCGGCAGGAGCCGGCTCTGGCtt                                                               <  1:92934/109‑1 (MQ=255)
                                                                                                                           ttGCGCTCGGCAGCACGGGCTGCGGCGTCTTCAGAGGCCGGGGCGTCTGCGACTACCGGGGCCGGAGCAGCGACCGGAGCAGCGGCAGGAGCCGGCTCTGGCTTGGCAGCAGGCGCAGGGGCGGCGGCTGCGGAGCCGGCGGCCTGACg                  >  4:117140/1‑149 (MQ=255)
                                                                                                                             gcgcTCGGCAGCACGGGCTGCGACGTCTTCAGAGGCCGGGGCGTCTGCGACTACCGGGGCCGGAGCAGCGACCGGAGCAGCGGCAGGAGCCGGCTCTGGCTTGGCAGCAGGCGCAGGGGCGGCGGCTGCGGAGCCGGCGGCCTGACgg                 <  1:175775/148‑1 (MQ=255)
                                                                                                                             gcgcTCGGCAGCACGGGCTGCGACGTCTTCAGAGGCCGGGGCGTCTGCGACTACCGGGGCCGGAGCAGCGACCGGAGCAGCGGCAGGAGCCGGCTCTGGCTTGGCAGCAGGCGCAGGGGCGGCGGCTGCGGAGCCGGCGGCCTGACgg                 <  1:170149/148‑1 (MQ=255)
                                                                                                                               gcTCGGCAGCACGGGCTGCGGCGTCTTCAGAGGCCGGGGCGTCTGCGACTACCGGGGCCGGAGCAGCGACCGGAGCAGCGGCAGGAGCCGGCTCTGGCTTGGCAGCAGGCGCAGGGGCGGCGGCTGCGGAGCCGGCGGCCTGACGGc                >  3:277895/1‑147 (MQ=255)
                                                                                                                                 tCGGCAGCACGGGCTGCGGCGTCTTCAGAGGCCGGGGCGTCTGCGACTACCGGGGCCGGAGCAGCGACCGGAGCAGCGGCAGGAGCCGGCTCTGGCTTGGCAGCAGGCGCAGGGGCGGCGGCTGCGGAGCCGGCGGCCTGACGGCGAGc            >  4:37281/1‑149 (MQ=255)
                                                                                                                                 tCGGCAGCACGGGCTGCGGCGTCTTCAGAGGCCGGGGCGTCTGCGACTACCGGGGCCGGAGCAGCGACCGGAGCAGCGGCAGGAGCCGGCTCTGGCTTGGCAGCAGGCGCAGGGGCGGCGGCTGCGGAGCCGGCGGCCTGACGGCGAGc            >  8:115281/1‑149 (MQ=255)
                                                                                                                                   ggCAGCACGGGCTGCGGCGTCTTCAGAGGCCGGGGCGTCTGCGACTACCGGGGCCGGAGCAGCGACCGGAGCAGCGGCAGGAGCCGGCTCTGGCTTGGCAGCAGGCGCAGGGGCGGCGGCTGCGGAGCCGGCGGCCTGACGGCGAGc            <  3:37281/147‑1 (MQ=255)
                                                                                                                                   ggCAGCACGGGCTGCGGCGTCTTCAGAGGCCGGGGCGTCTGCGACTACCGGGGCCGGAGCAGCGACCGGAGCAGCGGCAGGAGCCGGCTCTGGCTTGGCAGCAGGCGCAGGGGCGGCGGCTGCGGAGCCGGCGGCCTGACGGCGAGCCt          >  4:18832/1‑149 (MQ=255)
                                                                                                                                    gcagcaCGGGCTGCGGCGTCTTCAGAGGCCGGGGCGTCTGCGACTACCGGGGCCGGAGCAGCGACCGGAGCAGCGGCAGGAGCCGGCTCTGGCTTGGCAGCAGGCGCAGGGGCGGCGGCTGCGGAGCCGGCGGCCTGACGGCGAGCCTg         >  8:326632/1‑149 (MQ=255)
                                                                                                                                           gggCTGCGGCGTCTTCAGAGGCCGGGGCGTCTGCGACTACCGGGGCCGGAGCAGCGACCGGAGCAGCGGCAGGAGCCGGCTCTGGCTTGGCAGCAGGCGCAGGGGCGGCGGCTGCGGAGCCGGCGGCCTGACGGCGAGCCTGCTCTTCg  <  4:225334/149‑1 (MQ=255)
                                                                                                                                           gggCTGCGGCGTCTTCAGAGGCCGGGGCGTCTGCGACTACCGGGGCCGGAGCAGCGACCGGAGCAGCGGCAGGAGCCGGCTCTGGCTTGGCAGCAGGCGCAGGGGCGGCGGCTGCGGAGCCGGCGGCCTGACGGCGAGCCTGCTCTTCg  <  3:84107/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                            
GCACGCGGAGTCGGCGCCTTTTCCTTCTCCTTGACCTTGACCTTGATCGACACACGAGGCGCTTCGCCACGACGACGGTCGTCATCACGGGTACGGCTTTCGTTGCGACGGGTCTCGTCTTTCTTGCGCTCGGCAGCACGGGCTGCGGCGTCTTCAGAGGCCGGGGCGTCTGCGACTACCGGGGCCGGAGCAGCGACCGGAGCAGCGGCAGGAGCCGGCTCTGGCTTGGCAGCAGGCGCAGGGGCGGCGGCTGCGGAGCCGGCGGCCTGACGGCGAGCCTGCTCTTCG  >  NC_002947/5358084‑5358371

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑