Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,367,783 A→T 7.3% K102M (AAG→ATG)  PP_4720 → RNA‑binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,367,7830AT 7.3% 237.6 / 5.6 78K102M (AAG→ATG) PP_4720RNA‑binding protein
Reads supporting (aligned to +/- strand):  ref base A (35/37);  new base T (4/2);  total (39/39)
Fisher's exact test for biased strand distribution p-value = 6.75e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.81e-01

TCGGAAGACCGCGAAGAACGCGCCGAGACCATCGCCGAGCTGTGCAAGGCCGGCCGTGCCGAGCTGGTGCAGACCATCGGCAAGAAAGCCCTGATCTACCGCAAGAACCCACAGCCGAACAAGCAGCTGTCCAACATCCACCGTTACAAGTAACGGTAGCTCCGATCAGTGGCGCGGTGAACGCGCCCTGACCGGTACCGGCTGGGCCACCAGCACGATGCCGGAAAAGCCCAGCACCAGGAAGCAGAACATCTGCCAGCGCTCGCCCACGGAAATACCGTAGCGCAAGGTGTAATA  >  NC_002947/5367635‑5367931
                                                                                                                                                    |                                                                                                                                                    
tCGGAAGACCGCGAAGAACGCGCCGAGACCATCGCCGAGCTGTGCAAGGCCGGCCGTGCCGAGCTGGTGCAGACCATCGGCAAGAAAGCCCTGATCTACCGCAAGAACCCACAGCCGAACAAGCAGCTGTCCAACATCCACCGTTACaa                                                                                                                                                      >  3:248113/1‑149 (MQ=255)
  ggAAGACCGCGAAGAACGCGCCGAGACCATCGCCGAGCTGTGCAAGGCCGGCCGTGCCGAGCTGGTGCAGACCATCGGCAAGAAAGCCCTGATCTACCGCAAGAACCCACAGCCGAACAAGCAGCTGTCCAACATCCACCGTTACAAg                                                                                                                                                     <  1:222556/148‑1 (MQ=255)
  ggAAGACCGCGAAGAACGCGCCGAGACCATCGCCGAGCTGTGCAAGGCCGGCCGTGCCGAGCTGGTGC‑‑ACCATCGGCAAGAAAGCCCTGATCTACCGCAAGAACCCACAGCCGAACAAGCAGCTGTCCAACATCCACCGTTACAAGTaa                                                                                                                                                  >  5:21036/1‑149 (MQ=255)
   gAAGACCGCGAAGAACGCGCCGAGACCATCGCCGAGCTGTGCAAGGCCGGCCGTGCCGAGCTGGTGCAGACCATCGGCAAGAAAGCCCTGATCTACCGCAAGAACCCACAGCCGAACAAGCAGCTGTCCAACATCCACCGTTACAAGt                                                                                                                                                    >  7:140361/1‑148 (MQ=255)
         cgcgAAGAACGCGCCGAGACCATCGCCGAGCTGTGCAAGGCCGGCCGTGCCGAGCTGGTGCAGACCATCGGCAAGAAAGCCCTGATCTACCGCAAGAACCCACAGCCGAACAAGCAGCTGTCCAACATCCACCGTTACAAGTAACgg                                                                                                                                               >  6:12637/1‑147 (MQ=255)
         cgcgAAGAACGCGCCGAGACCATCGCCGAGCTGTGCAAGGCCGGCCGTGCCGAGCTGGTGCAGACCATCGGCAAGAAAGCCCTGATCTACCGCAAGAACCCACAGCCGAACAAGCAGCTGTCCAACATCCACCGTTACAAGTAACGGTa                                                                                                                                             <  7:32773/149‑1 (MQ=255)
           cgAAGATCGCGCCGAGACCATCGCCGAGCTGTGCAAGGCCGGCCGTGCCGAGCTGGTGCAGACCATCGGCAAGAAAGCCCTGATCTACCGCAAGAACCCACAGCCGAACAAGCAGCTGTCCAACATCCACCGTTACAAGTAACGGTAGc                                                                                                                                           <  7:50510/149‑1 (MQ=255)
           cgAAGAACGCGCCGAGACCATCGCCGAGCTGTGCAAGGCCGGCCGTGCCGAGCTGGTGCAGACCATCGGCAAGAAAGCCCTGATCTACCGCAAGAACCCACAGCCGAACAAGCAGCTGTCCAACATCCACCGTTACAAGTAACGGTAGc                                                                                                                                           >  4:121648/1‑149 (MQ=255)
             aagaaCGCGCCGAGACCATCGCCGAGCTGTGCAAGGCCGGCCGTGCCGAGCTGGTGCAGACCATCGGCAAGAAAGCCCTGATCTACCGCAAGAACCCACAGCCGAACAAGCAGCTGTCCAACATCCACCGTTACAAGTAACGGTAGCTc                                                                                                                                         <  3:121648/149‑1 (MQ=255)
                  cgcgCCGAGACCATCGCCGAGCTGTGCAAGGCCGGCCGTGCCGAGCTGGTGC‑‑ACCATCGGCAAGAAAGCCCTGATCTACCGCAAGAACCCACAGCCGAACAAGCAGCTGTCCAACATCCACCGTTACAAGTAACGGTAGCTCCGATCAg                                                                                                                                  <  6:21036/149‑1 (MQ=255)
                   gcgcCGAGACCATCGCCGAGCTGTGCAAGGCCGGCCGTGCCGAGCTGGTGCAGACCATCGGCAAGAAAGCCCTGATCTACCGCAAGAACCCACAGCCGAACAAGCAGCTGTCCAACATCCACCGTTACAAGTAACGGTAGCTCCGATCa                                                                                                                                   <  7:20475/149‑1 (MQ=255)
                     gcCGAGACCATCGCCGAGCTGTGCAAGGCCGGCCGTGCCGAGCTGGTGCAGACCATCGGCAAGAAAGCCCTGATCTACCGCAAGAACCCACAGCCGAACAAGCAGCTGTCCAACATCCACCGTTACAAGTAACGGTAGCTCCGATCAGt                                                                                                                                 >  2:99209/1‑149 (MQ=255)
                      ccGAGACCATCGCCGAGCTGTGCAAGGCCGGCCGTGCCGAGCTGGTGCAGACCATCGGCAAGAAAGCCCTGATCTACCGCAAGAACCCACAGCCGAACAAGCAGCTGTCCAACATCCACCGTTACaa                                                                                                                                                      <  1:189658/127‑1 (MQ=255)
                      ccGAGACCATCGCCGAGCTGTGCAAGGCCGGCCGTGCCGAGCTGGTGCAGACCATCGGCAAGAAAGCCCTGATCTACCGCAAGAACCCACAGCCGAACAAGCAGCTGTCCAACATCCACCGTTACaa                                                                                                                                                      >  2:189658/1‑127 (MQ=255)
                      ccGAGACCATCGCCGAGCTGTGCAAGGCCGGCCGTGCCGAGCTGGTGCAGACCATCGGCAAGAAAGCCCTGATCTACCGCAAGAACCCACAGCCGAACAAGCAGCTGTCCAACATCCACCGTTACAAGTAACGGTAGCTCCGATCAGTg                                                                                                                                <  7:297607/149‑1 (MQ=255)
                        gagaCCATCGCCGAGCTGTGCAAGGCCGGCCGTGCCGAGCTGGTGCAGACCATCGGCAAGAAAGCCCTGATCTACCGCAAGAACCCACAGCCGAACAAGCAGCTGTCCAACATCCACCGTTACAAGTAACGGTAGCTCCGATCAGTGgc                                                                                                                              >  1:90719/1‑149 (MQ=255)
                             cATCGCCGAGCTGTGCAAGGCCGGCCGTGCCGAGCTGGTGCAGACCATCGGCAAGAAAGCCCTGATCTACCGCAAGAACCCACAGCCGAACAAGCAGCTGTCCAACATCCACCGTTACAAGTAACGGTAGCTCCGATCAGTGGCGCGGt                                                                                                                         >  4:226091/1‑149 (MQ=255)
                             cATCGCCGAGCTGTGCAAGGCCGGCCGTGCCGAGCTGGTGCAGACCATCGGCAAGAAAGCCCTGATCTACCGCAAGAACCCACAGCCGAACAAGCAGCTGTCCAACATCCACCGTTACAAGTAACGGTAGCTCCGATCAGTGGCGCGGt                                                                                                                         >  4:125367/1‑149 (MQ=255)
                                 gCCGAGCTGTGCAAGGCCGGCCGTGCCGAGCTGGTGCAGACCATCGGCAAGAAAGCCCTGATCTACCGCAAGAACCCACAGCCGAACAAGCAGCTGTCCAACATCCACCGTTACAAGTAACGGTAGCTCCGATCAGTGGCGCGGTGAAc                                                                                                                     <  3:96130/149‑1 (MQ=255)
                                          tgCAAGGCCGGCCGTGCCGAGCTGGTGCAGACCATCGGCAAGAAAGCCCTGATCTACCGCAAGAACCCACAGCCGAACAAGCAGCTGTCCAACATCCACCGTTACAAGTAACGGTAGCTCCGATCAGTGGCGCGGTGAACGCGCCCt                                                                                                              <  8:215404/147‑1 (MQ=255)
                                          tgCAAGGCCGGCCGTGCCGAGCTGGTGCAGACCATCGGCAAGAAAGCCCTGATCTACCGCAAGAACCCACAGCCGAACAAGCAGCTGTCCAACATCCACCGTTACAAGTAACGGTAGCTCCGATCAGTGGCGCGGTGAACGCGCCCTGa                                                                                                            >  8:323580/1‑149 (MQ=255)
                                            cAAGGCCGGCCGTGCCGAGCTGGTGCAGACCATCGGCAAGAAAGCCCTGATCTACCGCAAGAACCCACAGCCGAACAAGCAGCTGTCCAACATCCACCGTTACATGTATCGGTAGCTCGTATCAGTGGCGCGGTGAACGCGCCCTGAc                                                                                                           <  6:212801/148‑1 (MQ=255)
                                             aaGGCCGGCCGTGCCGAGCTGGTGCAGACCATCGGCAAGAAAGCCCTGATCTACCGCAAGAACCCACAGCCGAACAAGCAGCTGTCCAACATCCACCGTTACAAGTAACGGTAGCTCCGATCAGTGGCGCGGTGAACGCGCCCTGACCg                                                                                                         >  6:345500/1‑149 (MQ=255)
                                             aaGGCCGGCCGTGCCGAGCTGGTGCAGACCATCGGCAAGAAAGCCCTGATCTACCGCAAGAACCCACAGCCGAACAAGCAGCTGTCCAACATCCACCGTTACAAGTAACGGTAGCTCCGATCAGTGGCGCGGTGAACGCGCCCTGACCg                                                                                                         >  3:91855/1‑149 (MQ=255)
                                                 ccggccgTGCCGAGCTGGTGCAGACCATCGGCAAGAAAGCCCTGATCTACCGCAAGAACCCACAGCCGAACAAGCAGCTGTCCAACATCCACCGTTACAAGTAACGGTAGCTCCGATCAGTGGCGCGGTGAACGCGCCCTGACCGGTAc                                                                                                     <  2:90719/149‑1 (MQ=255)
                                                     ccgTGCCGAGCTGGTGCAGACCATCGGCAAGAAAGCCCTGATCTACCGCAAGAACCCACAGCCGAACAAGCAGCTGTCCAACATCCACCGTTACAAGTAACGGTAGCTCCGATCAGTCGCGCGGTGAACGCGCCCAGACCGGTACCGGc                                                                                                 >  3:248921/1‑149 (MQ=255)
                                                      cgTGCCGAGCTGGTGCAGACCATCGGCAAGAAAGCCCTGATCTACCGCAAGAACCCACAGCCGAACAAGGAGCTGTCCAACATCCACCGTTACAAGTAACGGTAGCTCCGATCAGTGGCGCGGTGAACGCGCCCTGACCGGTACCGGCt                                                                                                >  1:116109/1‑149 (MQ=255)
                                                        tGCCGAGCTGGTGCAGACCATCGGCAAGAAAGCCCTGATCTACCGCAAGAACCCACAGCCGAACAAGCAGCTGTCCAACATCCACCGTTACAAGTAACGGTAGCTCCGATCAGTGGCGCGGTGAACGCGCCCTGACCGGTACCGGCTgg                                                                                              <  5:12637/149‑1 (MQ=255)
                                                        tGCCGAGCTGGTGCAGACCATCGGCAAGAAAGCCCTGATCTACCGCAAGAACCCACAGCCGAACAAGCAGCTGTCCAACATCCACCGTTACAAGTAACGGTAGCTCCGATCAGTGGCGCGGTAAACGCGCCCTGACCGGTATCGGCTgg                                                                                              <  4:248113/149‑1 (MQ=255)
                                                          ccGAGCTGGTGCAGACCATCGGCAAGAAAGCCCTGATCTACCGAAAGAACCCACAGCCGAACAAGCAGCTGTCCAACATCCACCGTTACAAGTAACGGTAGCTCCGATCAGTGGCGCGGTGAACGCGCCCTGACCGGTACCGGCTGGGc                                                                                            <  4:91855/149‑1 (MQ=255)
                                                                     cAGACCATCGGCAAGAAAGCCCTGATCTACCGCAAGAACCCACAGCCGAACAAGCAGCTGTCCAACATCCACCGTTACAAGTAACGGTAGCTCCGATCAGTGGCGCGCTGAACGCGCCCTGACCGGTACCGGCTGGGCCACCAGCACGa                                                                                 >  5:319119/1‑149 (MQ=255)
                                                                      aGACCATCGGCAAGAAAGCCCTGATCTACCGCAAGAACCCACAGCCGAACAAGCAGCTGTCCAACATCCACCGTTACAAGTAACGGTAGCTCCGATCAGTGGCGCGGTGAACGCGCCCTGACCGGTACCGGCTGGGCCACCAGCACGAt                                                                                <  4:248921/149‑1 (MQ=255)
                                                                          cATCGGCAAGAAAGCCCTGATCTACCGCAAGAACCCACAGCCGAACAAGCAGCTGTCCAACATCCACCGTTACAAGTAACGGTAGCTCCGATCAGTg                                                                                                                                >  7:338768/1‑97 (MQ=255)
                                                                          cATCGGCAAGAAAGCCCTGATCTACCGCAAGAACCCACAGCCGAACAAGCAGCTGTCCAACATCCACCGTTACAAGTAACGGTAGCTCCGATCAGTg                                                                                                                                <  8:338768/97‑1 (MQ=255)
                                                                          cATCGGCAAGAAAGCCCTGATCTACCGCAAGAACCCACAGCCGAACAAGCAGCTGTCCAACATCCACCGTTACAAGTAACGGTAGCTCCGATCAGTGGCGCGGTGAACGCGCCCTGACCGGTACCGGCTGGGCCACCAGCACGATGCCg                                                                            <  3:226091/149‑1 (MQ=255)
                                                                          cATCGGCAAGAAAGCCCTGATCTACCGCAAGAACCCACAGCCGAACAAGCAGCTGTCCAACATCCACCGTTACAAGTAACGGTAGCTCCGATCAGTGGCGCGGTGAACGCGCCCTGACCGGTACCGGCTGGGCCACCAGCACGATGCCg                                                                            <  1:99209/149‑1 (MQ=255)
                                                                               gCAAGAAAGCCCTGATCTACAGCAAGAACCCACAGCCGAACAAGCAGCTGTCCAACATCCACCGTTACAAGTAACGGTAGCTCCGATCAGTGGCGCGGTGAACGCGCCCTGACCGGTACCGGCTGGGCCACCAGCACGATGCCGGaaaa                                                                       >  8:290591/1‑149 (MQ=255)
                                                                                 aaGAAAGCCCTGATCTACCGAAAGAACCAACTGCCGAACAAGCAGCTGTCCAACATCCACCGTTACAATTAACGGTAGCTCCTATCAGTGGCGCTGTGAACGCGCCCTGACCGGTACCGGCTTGGCCACCAGCACGATGCCGGAAAAGc                                                                     <  2:328717/149‑1 (MQ=255)
                                                                                          cTGATCTACCGCAAGAACCCACAGCCGAACAAGCAGCTGTCCAACATCCACCGTTACAAGTAACGGTAGCTCCGATCAGTGGCGCGGTGAAc                                                                                                                     <  2:47165/92‑1 (MQ=255)
                                                                                          cTGATCTACCGCAAGAACCCACAGCCGAACAAGCAGCTGTCCAACATCCACCGTTACAAGTAACGGTAGCTCCGATCAGTGGCGCGGTGAAc                                                                                                                     >  1:47165/1‑92 (MQ=255)
                                                                                           tGATCTACCGCAAGAACCCACAGCCGAACAAGCAGCTGTCCAACATCCACCGTTACAAGTAACGGTAGCTCCGATCAGTGGCGCGGTGAACGCGCCCTGACCGGTACCGGCTGGGCCACCAGCACGATGCCGGAAAAGCCCAGCACCAg                                                           >  8:748/1‑149 (MQ=255)
                                                                                             aTCTACCGCAAGAACCCACAGCCGAACAAGCAGCTGTCCAACATCCACCGTTACAAGTAACGGTAGCTCCGATCAGTGGCGCGGTGAACGCGCCCTGACCGGTACCGGCTGGGCCACCAGCACGATGCCGGAAAAGCCCAGCACCAg                                                           <  3:125367/147‑1 (MQ=255)
                                                                                               cTACCGCAAGAACCCACAGCCGAACAAGCAGCTGTCCAACATCCACCGTTACAAGTAACGGTAGCTCCGATCAGTGGCGCGGTGAACGCGCCCTGACCGGTACCGGCTGGGCCACCAGCACGATGCCGGAAAAGCCCAGCACCAGGAAg                                                       >  6:311653/1‑149 (MQ=255)
                                                                                                  ccGCAAGAACCCACAGCCCAACAAGCAGCTGTCCAACATCCACCGTTACAAGTAACGGTAGCTCCGATCAGTGGCGCGGTGAACGCGCCCTGACCGGTACCGGCTGGGCCACCAGCACGATGCCGGAAAAGCCCAGCACCAGGAAg                                                       <  8:140361/146‑1 (MQ=255)
                                                                                                     cAAGAACCCACAGCCGAACAAGCAGCTGTCCAACATCCACCGTTACAAGTAACGGTAGCTCCGATCa                                                                                                                                   >  6:234072/1‑67 (MQ=255)
                                                                                                     cAAGAACCCACAGCCGAACAAGCAGCTGTCCAACATCCACCGTTACAAGTAACGGTAGCTCCGATCa                                                                                                                                   <  5:234072/67‑1 (MQ=255)
                                                                                                     cAAGAACCCACAGCCGAACAAGCAGCTGTCCAACATCCACCGTTACAAGTAACGGTAGCTCCGATCAGTGGCGCGGTGAACGCGCCCTGACCGGTACCGGCTGGGCCACCAGCACGATGCCGGAAAAGCCCAGCACCAGGAAGCAGaa                                                  >  5:347766/1‑148 (MQ=255)
                                                                                                        gAACCCACAGCCGAACAAGCAGCTGTCCAACATCCACCGTTACAAGTAACGGTAGCTCCGATCAGTGGCGCGGTGAACGCGCCCTGACCGGTACCGGCTGGGCCACCAGCACGATGCCGGAAAAGCCCAGCACCAGGAAGCa                                                     <  6:148588/142‑1 (MQ=255)
                                                                                                        gAACCCACAGCCGAACAAGCAGCTGTCCAACATCCACCGTTACAAGTAACGGTAGCTCCGATCAGTGGCGCGGTGAACGCGCCCTGACCGGTACCGGCTGGGCCACCAGCACGATGCCGGAAAAGCCCAGCACCAGGAAGCAGagatag                                              >  5:148588/1‑144 (MQ=255)
                                                                                                         aaCCCACAGCCGAACAAGCAGCTGTCCAACATCCACCGTTACAAGTAACGGTAGCTCCGATCAGTGGCGCGGTGAACGCGCCCTGACCGGTACCGGCTGGGCCACCAGCACGATGCCGGAAAAGCCCAGCACCAGGAAGCAGAACATCt                                             <  2:116109/149‑1 (MQ=255)
                                                                                                             cacaGCCGAACAAGCAGCTGTCCAACATCCACCTTTACAAGTAACGGTAGCTCCGATCAGTGGCGCGGTGAACGCGCACTGACCGGTACCGGCTGGGCCACCAGCACGATGCCGGAAAAGCCCAGCACCAGGAAGCAGAACATCTGCCa                                         <  7:290591/149‑1 (MQ=255)
                                                                                                              acaGCCGAACAAGCAGCTGTCCAACATCCACCGTTACAAGTAACGGTAGCTCCGATCAGTGGCGCGGTGAACGCGCCCTGACCGGTACCGGCTGGGCCACCAGCACGATGCCGGAAAAGCCCa                                                                  >  8:290242/1‑123 (MQ=255)
                                                                                                              acaGCCGAACAAGCAGCTGTCCAACATCCACCGTTACAAGTAACGGTAGCTCCGATCAGTGGCGCGGTGAACGCGCCCTGACCGGTACCGGCTGGGCCACCAGCACGATGCCGGAAAAGCCCa                                                                  <  7:290242/123‑1 (MQ=255)
                                                                                                              acaGCCGAACAAGCAGCTGTCCAACATCCACCGTTACAAGTAACGGTAGCTCCGATCAGTGGCGCGGTGAACGCGCCCTGACCGGTACCGGCTGGGCCACCAGCACGATGCCGGAAAAGCCCAGCACCAGGAAGCAGAACATCTGCCAg                                        >  7:343043/1‑149 (MQ=255)
                                                                                                              acaGCCGAACAAGCAGCTGTCCAACATCCACCGTTACAAGTAACGGTAGCTCCGATCAGTGGCGCGGTGAACGCGCCCTGACCGGTACCGGCTGGGCCACCAGCACGATGCCGGAAAAGCCCAGCACCAGGAAGCAGAACATCTGCCAg                                        <  5:311653/149‑1 (MQ=255)
                                                                                                                    gAACAAGCAGCTGTCCAACATCCACCGTTACAAGTAACGGTAGCTCCGATCAGTGGCGCGGTGAACGCGCCCTGACCGGTACCGGCTGGGCCACCAGCACGATGCCGGAAAAGCCCAGCACCAGGAAGCAGAACATCTGCCAGCGCTCg                                  >  5:12679/1‑149 (MQ=255)
                                                                                                                    gAACAAGCAGCTGTCCAACATCCACCGTTACAAGTAACGGTAGCTCCGATCAGTGGCGCGGTGAACGCGCCCTGACCGGTACCGGCTGGGCCACCAGCACGATGCCGGAAAAGCCCAGCACCAGGAAGCAGAACATCTGCCAGCGCTCg                                  <  7:104630/149‑1 (MQ=255)
                                                                                                                     aaCAAGCAGCTGTCCAACATCCACCGTTACAAGTAACGGTAGCTCCGATCAGTGGCGCGGTGAACGCGCCCTGACCGGTACCGGCTGGGCCACCAGCACGATGCCGGAAAAGCCCAGCACCAGGAAGCAGAACATCTGCCAGCGCTCGc                                 <  6:319119/149‑1 (MQ=255)
                                                                                                                      aCAAGCAGCTGTCCAACATCCACCGTTACAAGTAACGGTAGCTCCGATCAGTGGCGCGGTGAACGCGCCCTGACCGGTACCGGCTGGGCCACCAGCACGATGCCGGAAAAGCCCAGCACCAGGAAGCAGAACATCTGCCAGCGCTCGcc                                >  1:72526/1‑149 (MQ=255)
                                                                                                                        aaGCAGCTGTCCAACATCCACCGTTACAAGTAACGGTAGCTCCGATCAGTGGCGCGGTGAACGCGCCCTGACCGGTACCGGCTGGGCCACCAGCACGATGCCGGAAAAGCCCAGCACCAGGAAGCAGAACATCTGCCAGCGCTCGCCCa                              >  4:123352/1‑149 (MQ=255)
                                                                                                                          gcagcTGTCCAACATCCACCGTTACAAGTAACGGTAGCTCCGATCAGTGGCGCGGTGAACGCGCCCTGACCGGTACCGGCTgg                                                                                              <  7:160014/83‑1 (MQ=255)
                                                                                                                          gcagcTGTCCAACATCCACCGTTACAAGTAACGGTAGCTCCGATCAGTGGCGCGGTGAACGCGCCCTGACCGGTACCGGCTgg                                                                                              >  8:160014/1‑83 (MQ=255)
                                                                                                                                   cAACATCCACCGTTACAAGTAACGGTAGCTCCGATCAGTGGCGCGGTGAACGCGCCCTGACCGGTACCGGCTGGGCCACCAGCACGATGCCGGAAAAGCACAGCACCAGGAAGCAGAACATCTGCCAGCGCTCGCCCACGGAAATAcc                    >  8:335567/1‑148 (MQ=255)
                                                                                                                                   cAACATCCACCGTTACAAGTAACGGTAGCTACGATCAGTGGCGCGGTGAACGCGCCCTGACCGGTACCGGCTGGGCCACCAGCACGATGCCGGAAAAGCCCAGCACCAGGAAGCAGAACATCTGCCAGCGCTCGCCCACGGAAATAcc                    >  8:139851/1‑148 (MQ=255)
                                                                                                                                    aaCATCCACCGTTACAAGTAACGGTAGCTCCGATCAGTGGCGCGGTGAATGCGCCCTGACCGGTACCGGCTGGGCCACCAGCACGATGCCGGAAAAGCCCAGCACCAGGAAGCAGAACATCTGCCAGCGCTCGCCCACGCAAATACCg                   >  6:306435/1‑148 (MQ=255)
                                                                                                                                    aaCATCCACCGTTACAAGTAACGGTAGCTCCGATCAGTGGCGCGGTGAACGCGCCCTGACCGGTACCGGCTGGGCCACCAGCACGATGCCGGAAAAGCCCAGCACCAGGAAGCAGAACATCTGCCAGCGCTCGCCCACGGAAATACCg                   >  3:299613/1‑148 (MQ=255)
                                                                                                                                     ggagctaCCGTTACTTGTAACGGTAGCTCCGATCAGTGGCGCGGTGAACGCGCCCTGACCGGTACCGGCTGGGCCACCAGCACGATGCCGGAAAAGCCCAGCACCAGGAAGCAGAACATCTGCCAGCGCTCGCCCACGGAAATACCg                   >  8:266047/7‑147 (MQ=255)
                                                                                                                                     aCATCCACCGTTACAAGTAACGGTAGCTCCGATCAGTGGCGCGGTGAACGCGCCCTGACCGGTACCGGCTGGGCCACCAGCACGATGCCGGAAAAGCCCAGCACCAGGAAGCAGAACATCTGCCAGCGCTCGCCCACGGAAATACCGTa                 <  7:323580/149‑1 (MQ=255)
                                                                                                                                       aTCCACCGTTACAAGTAACGGTAGCTCCGATCAGTGGCGCGGTGAACGCGCCCTGACCGGTACCGGCTGGGCCACCAGCACGATGCCGGAAAAGCCCAGCACCAGGAAGCAGAACATCTGCCAGCGCTCGCCCACGGAAATACCGTAgc               <  2:206719/149‑1 (MQ=255)
                                                                                                                                         ccACCGTTACAAGTAACGGTAGCTCCGATCAGTGGCGCGGTGAACGCGCCCTGACCGGTACCGGCTGGGCCACCAGCACGATGCCGGAAAAGCCCAGCACCAGGAAGCAGAACATCTGCCAGCGCTCGCCCACGGAAATACCGTAgcg              >  6:366993/1‑148 (MQ=255)
                                                                                                                                          taCCGTTACTTGTAACGGTAGCTCCGATCAGTGGCGCGGTGAACGCGCCCTGACCGGTACCGGCTGGGCCACCAGCACGATGCCGGAAAAGCCCAGCACCAGGAAGCAGAACATCTGCCAGCGCTCGCCCACGGAAATACCGTAgcgc             >  3:172481/2‑148 (MQ=255)
                                                                                                                                           aCCGTTACTTGTAACGGTAGCTCCGATCAGTGGCGCGGTGAACGCGCCCTGACCGGTACCGGCTGGGCCACCAGCACGATGCCGGAAAAGCCCAGCACCa                                                            >  6:304922/1‑100 (MQ=255)
                                                                                                                                           aCCGTTACTTGTAACGGTAGCTCCGATCAGTGGCGCGGTGAACGCGCCCTGACCGGTACCGGCTGGGCCACCAGCACGATGCCGGAAAAGCCCAGCACCa                                                            <  5:304922/100‑1 (MQ=255)
                                                                                                                                           aCCGTTACTTGTAACGGTAGCTCCGATCAGTGGCGCGGTGAACGCGCCCTGACCGGTACCGGCTGGGCCACCAGCACGATGCCGGAAAAGCCCAGCACCAGGAAGCAGAACATCTGCCAGCGCTCGCCCACGGAAATACCGTAgcgc             >  5:51894/1‑147 (MQ=255)
                                                                                                                                            ccGTTACAAGTAACGGTAGCTCCGATCAGTGGCGCGGTGAACGCGCCCTGACCGGTACCGGCTGGGCCACCAGCACGATGCCGGAAAAGCCCAGCACCAGGAAGCAGAACATCTGCCAGCGCTCGCCCACGGAAATACCGTAGCGCAAg          <  6:12679/149‑1 (MQ=255)
                                                                                                                                            ccGTTACAAGTAACGGTAGCTCCGATCAGTGGCGCGGTGAACGCGCCCTGACCGGTACCGGCTGGGCCACCAGCACGATGCCGGAAAAGCCCAGCACCAGGAAGCAGAACATCTGCCAGCGCTCGCCCACGGAAATACCGTAGCGCAAg          <  2:72526/149‑1 (MQ=255)
                                                                                                                                               ttACAAGTAACGGTAGCTCCGATCAGTGGCGCGGTGAACGCGCCCTGACCGGTACCGGCTGGGCCACCAGCACGATGCCGGAAAAGCCCAGCACCAGGAAGCAGAACATCTGCCAGCGCTCGCCCACGGAAATACCGTa                 >  6:324576/1‑139 (MQ=255)
                                                                                                                                               ttACAAGTAACGGTAGCTCCGATCAGTGGCGCGGTGAACGCGCCCTGACCGGTACCGGCTGGGCCACCAGCACGATGCCGGAAAAGCCCAGCACCAGGAAGCAGAACATCTGCCAGCGCTCGCCCACGGAAATACCGTa                 <  5:324576/139‑1 (MQ=255)
                                                                                                                                                 aCAAGTAACGGTAGCTCCGATCAGTGGCGCGGTGAACGCGCCCTGACCGGTACCGGCTGGGCCACCAGCACGATGCCGGAAAAGCCCAGCACCAGGAAGCAGAACATc                                              >  2:99922/1‑108 (MQ=255)
                                                                                                                                                 aCAAGTAACGGTAGCTCCGATCAGTGGCGCGGTGAACGCGCCCTGACCGGTACCGGCTGGGCCACCAGCACGATGCCGGAAAAGCCCAGCACCAGGAAGCAGAACATc                                              <  1:99922/108‑1 (MQ=255)
                                                                                                                                                  cAAGTAACGGTAGCTCCGATCAGTGGCGCGGTGAACGCGCCCTGACCGGTACCGGCTGGGCCACCAGCACGATGCCGGAAAAGCGCAGCACCAGGAAGCAGAACATCTGCCAGCGCTCGCCCACGGAAATACCGTAGCGCAAGGTGTa     <  7:335567/148‑1 (MQ=255)
                                                                                                                                                   aaGTAACGGTAGCTCCGATCAGTGGCGCGGTGAACGCGCCCTGACCGGTACCGGCTGGGCCACCAGCACGATGCCGGAAAAGCCCAGCACCAGGAAGCAGAACATCTGCCAGCGCTCGCCCACGGAAATACCGTAGCGCAAGGTGTaa    >  8:197735/1‑148 (MQ=255)
                                                                                                                                                    aGTAACGGTAGCTCCGATCAGTGGCGCGGTGAACGCGCCCTGACCGGTACCGGCTGGGCCACCAGCACGATGCCGGAAAAGCCCAGCACCAGGAAGCAGAACATCTGCCAGCGCTCGCCCACGGAAATACCGTAGCGCAAGGTGTAATa  <  5:345500/149‑1 (MQ=255)
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TCGGAAGACCGCGAAGAACGCGCCGAGACCATCGCCGAGCTGTGCAAGGCCGGCCGTGCCGAGCTGGTGCAGACCATCGGCAAGAAAGCCCTGATCTACCGCAAGAACCCACAGCCGAACAAGCAGCTGTCCAACATCCACCGTTACAAGTAACGGTAGCTCCGATCAGTGGCGCGGTGAACGCGCCCTGACCGGTACCGGCTGGGCCACCAGCACGATGCCGGAAAAGCCCAGCACCAGGAAGCAGAACATCTGCCAGCGCTCGCCCACGGAAATACCGTAGCGCAAGGTGTAATA  >  NC_002947/5367635‑5367931

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑