Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,391,824	G→T	7.9%	L24L (CTC→CTA) ‡	PP_5709 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,391,824	0	G	T	7.9%	196.9 / 5.1	63	L24L (CTC→CTA) ‡	PP_5709	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (28/30);  new base T (2/3);  total (30/33)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.06e-01

ACCGCATCAGGGAGCTTCGTCAGCCCTTCGTTCTGCGTCAACTTGTAAGCTAGGCTGGCAACCACTTCCTCACTATCACCGACTAAACGGCCATCCAGAGTGAACGGTTTGC‑‑CAGGGTAGGTCGACTGCAACTGGGAGACGGCTGCGAGCAGCCCTTCAGATGCCTTTTGGATTTTCGCCTCGGTCTTCAGGTCAATCAAATCTCGCGATGCTCGCATAGCCTTGCCCTATGACCGGCTACATCAGAATCTTGTTTTCACTTCAATAACCTTGCCATGCAAGCAGTATCATTGA  >  NC_002947/5391678‑5391971                                                                                                                                                     |                                                                                                                                                    aCCGCATCAGGGAGCTTCGTCAGCCCTTCGTTCTGCGTCAACTTGTAAGCTAGGCTGGCAACCACTTCCTCACTATCACCGACTAAACGGCCATCCAGAGTGAACGGTTTGC‑‑CAGGGTAGGTCGACTGCAACTGGGAGACGGCTGCGag                                                                                                                                                   <  8:300006/149‑1 (MQ=255)         aGGGAGCTTCGTCAGCCCTTCGTTCTGCGTCAACTTGTAAGCTAGGCTGGCAACCACTTCCTCACTATCACCGACTAAACGGCCATCCAGAGTGAACGGTTTGC‑‑CAGGGTAGGTCGACTGCAACTGGGAGACGGCTGCTCGCAGccgt                                                                                                                                            <  5:117046/148‑3 (MQ=255)         aGGGAGCTTCGTCAGCCCTTCGTTCTGCGTCAACTTGTAAGCTAGGCTGGCAACCACTTCCTCACTATCACCGACTAAACGGCCATCCAGAGTGAACGGTTTGC‑‑CAGGGTAGGTCGACTGCAACTGGGAGACGGCTGCGAGCAGCCCtt                                                                                                                                           >  2:123774/1‑149 (MQ=255)         aGGGAGCTTCGTCAGCCCTTCGTTCTGCGTCAACTTGTAAGCTAGGCTGGCAACCACTTCCTCACTATCACCGACTAAACGGCCATCCAGAGTGAACGGTTTGC‑‑CAGGGTAGGTCGACTGCAACTGGGAGACGGCTGCGAGCAGCCCt                                                                                                                                            <  5:110443/148‑1 (MQ=255)          gggAGCTTCGTCAGCCCTTCGTTCTGCGTCAACTTGTAAGCTAGGCTGGCAACCACTTCCTCACTATCACCGACTAAACGGCCATCCAGAGTGAACGGTTTGC‑‑CAGGGTAGGTCGACTGCAACTGGGAGACGGCTGCTCGCAGccgtct                                                                                                                                          <  8:24886/149‑5 (MQ=255)           ggAGCTTCGTCAGCCCTTCGTTCTGCGTCAACTTGTAAGCTAGGCTGGCAACCACTTCCTCACTATCACCGACTAAACGGCCATCCAGAGTGAACGGTTTGC‑‑CAGGGTAGGTCGACTGCAACTGGGAGACGGCTGCGAGCAGCCCTTCa                                                                                                                                         <  3:118177/149‑1 (MQ=255)                      aGCCCTTCGTTCTGCGTCAACTTGTAAGCTAGGCTGGCAACCACTTCCTCACTATCACCGACTAAACGGCCATCCAGAGTGAACGGTTTGC‑‑CAGGGTAGGTCGACTGCAACTGGGAGACGGCTGCGAGCAGCCCTTCAGATGCCtttt                                                                                                                               <  6:88012/148‑1 (MQ=255)                       gCCCTTCGTTCTGCGTCAACTTGTAAGCTAGGCTGGCAACCACTTCCTCACTATCACCGACTAAACGGCCATCCAGAGTGAACGGTTTGC‑‑CAGGGTAGGTCGACTGCAACTGTGAGACGGCTGCGAGCAGCCCTTCAGATGCCTTTTgg                                                                                                                             <  1:72187/149‑1 (MQ=255)                        cccTTCGTTCTGCGTCAACTTGTAAGCTAGGCTGGCAACCACTTCCTCACTATCACCGACTAAACGGCCATCCAGAGTGAACGGTTTGC‑‑CAGGGTAGGTCGACTGCAACTGGGAGACGGCTGCGAGCAGCCCTTCAGATGCCTTTTgta                                                                                                                            >  5:70136/1‑147 (MQ=255)                        cccTTCGTTCTGCGTCAACTTGTAAGCTAGGCTGGCAACCACTTCCTCACTATCACCGACTAAACGGCCATCCAGAGTGAACGGTTTGC‑‑CAGGGTAGGTCGACTGCAACTGGGAGACGGCTGCGAGCAGCCCTTCAGATGCCTTTTGGa                                                                                                                            >  4:250806/1‑149 (MQ=255)                          cTTCGTTCTGCGTCAACTTGTAAGCTAGGCTGGCAACCACTTCCTCACTATCACCGACTAAACGGCCATCCAGAGTGAACGGTTTGC‑‑CAGGGTAGGTCGACTGCAACTGGGAGACGGCTGCGAGCAGCCCTTCAGATGCATTTTGGAtt                                                                                                                          >  7:146231/1‑149 (MQ=255)                           ttCGTTCTGCGTCAACTTGTAAGCTAGGCTGGCAACCACTTCCTCACTATCACCGACTAAACGGCCATCCAGAGTGAACGGTTTGC‑‑CAGGGTAGGTCGACTGCAACTCGGAGACGGCTGCGAGCAGCCCTTCAGATGCCTTTTGGAttt                                                                                                                         >  3:37822/1‑149 (MQ=255)                             ctttCTGCGTCAACTTTTAAGCTAGGCTGTCAACCACTTACTCACTATCACCTACTAAACGGCCATCCAGATTGAACGTTTTGC‑‑CAGTGTAGGTCTACTTTAACTGGGAGACGGCTGCTAGCAGTCCTTCAGATGCCTTTTGGAtttt                                                                                                                        <  7:235901/146‑1 (MQ=255)                                    cGTCAACTTGTAAGCTAGGCTGGCAACCACTTCCTCACTATCACCGACTAAACGGCCATCCAGAGTGAACGGTTTGC‑‑CAGGGTAGGTCGACTGCAACTGGGAGACGGCTGCGAGCAGCCCTTCAGATGCCTTTTGGATTTTCGCCt                                                                                                                   <  8:146231/146‑1 (MQ=255)                                          cTTGTAAGCTAGGCTGGCAACCACTTCCTCACTTTCACCGACTAAACGGCCATCCAGAGTGAACGGTTTGC‑‑CAGGGTAGGTCGACTGCAACTGGGAGACGGCTGCGAGCAGCCCTTCAGATGCATGTTGGATTTTCGCCGCGGTCtt                                                                                                            >  4:217889/1‑147 (MQ=255)                                          cTTGTAAGCTAGGCTGGCAACCACTTCCTCACTATCACCGACTAAACGGCCATCCAGAGTGAACGGTTTGC‑‑CAGGGTAGGTCGACTGCAACTGGGAGACGGCTGCGAGCAGCCCTTCAGATGCCTTTTGGATTTTCGCCTCGGTCtt                                                                                                            >  4:156761/1‑147 (MQ=255)                                          cTTGTAAGCTAGGCTGGCAACCACTTCCTCACTATCACCGACTAAACGGCCATCCAGAGTGAACGGTTTGC‑‑CAGGGTAGGTCGACTGCAACTGGGAGACGGCTGCGAGCAGCCCTTCAGATGCCTTTTGGATTTTCGCCTCGGTCTTCa                                                                                                          <  2:49942/149‑1 (MQ=255)                                           ttGTAAGCTAGGCTGGCAACCACTTCCTCACTATCACCGACTAAACGGCCATCCAGAGTGAACGGTTTGC‑‑CAGGGTAGGTCGACTGCAACTGGGAGACGGCTGCGAGCAGCCCTTCAGATGCCTTTTGGATTTTCGCCTCGGTCTTCAg                                                                                                         >  1:240706/1‑149 (MQ=255)                                             gTAAGCTAGGCTGGCAACCACTTCCTCACTATCACCGACTAAACGGCCATCCAGAGTGAACGGTTTGC‑‑CAGGGTAGGTCGACTGCAACTGGGAGACGGCTGCGAGCAGCCCTTCAGATGCCTTTTGGATTTTCGCCTCGGTCTTCAg                                                                                                         <  3:156761/147‑1 (MQ=255)                                              tAAGCTAGGCTGGCAACCACTTCCTCACTATCACCGACTAAACGGCCATCCAGAGTGAACGGTTTGC‑‑CAGGGTAGGTCGACTGCAACTGGGAGACGGCTGCGAGCAGCCCTTCAGATGCCTTTTGGATTTTCGCCTCGGTCTTCAGGt                                                                                                       <  3:250806/148‑1 (MQ=255)                                                aGCTAGGCTGGCAACCACTTCCTCACTATCACCGACTAAACGGCCATCCAGAGTGAACGGTTTGC‑‑CAGGGTAGGTCGACTGCAACTGGGAGACGGCTGCGAGCAGCCCTTCAGATGCCTTTTGGATTTTCGCCTCGGTCTTCAGGtcaa                                                                                                    >  7:211265/1‑149 (MQ=255)                                                    aGGCTGGCAACCACTTCCTCACTATCACCGACTAAACGGCCATCCAGAGTGAACGGTTTGC‑‑CAGGGTAGGTCGACTGCAACTGGGAGACGGCTGCGAGCAGCCCTTCAGATGCCTTTTGGATTTTCGCCTCGGTCTTCAGGtcaatca                                                                                                 >  4:209074/1‑148 (MQ=255)                                                    aGGCTGGCAACCACTTCCTCACTATCACCGACTAAACGGCCATCCAGAGTGAACGGTTTGC‑‑CAGGGTAGGTCGACTGCAACTGGGAGACGGCTGCGAGCAGCCCTTCAGATGCCTTTTGGATTTTCGCCTCGGTCTTCAGGtcaatca                                                                                                 >  2:14789/1‑148 (MQ=255)                                                             aCCACTTCCTCACTATCACCGACTAAACGGCCATCCAGAGTGAACGGTTTGC‑‑CAGGGTAGGTCGACTGCAACTGGGAGACGGCTGCGAGCAGCCCTTCAGATGCCTTTTGGATTTTCGCCTCGGTCTTCAGGTCAATCAAATCTCGCGa                                                                                       <  1:14789/149‑1 (MQ=255)                                                               cACTTCCTCACTATCACCGACTAAACGGCCATCCAGAGTGAACGGTTTGC‑‑CAGGGTAGGTCGACTGCAACTGGGAGACGGCTGCGAGCAGCCCTTCAGATGCCTTTTGGATTTTCGCCTCGGTCTTCAGGTCAATCAAATCTCGCGATg                                                                                     >  4:13493/1‑149 (MQ=255)                                                                      tCACTATCACCGACTAAACGGCCATCCAGAGTTAACGGTTTGC‑‑CAGGGTAGGTCGACTGCAACTGGGAGACGGCTGCGAGCAGCCCTTCAGATGCCTTTTGGATTTTCGCCTCGGTCTTCAGGTCAATCAAATCTCGCGATGCTCGCAt                                                                              <  6:377968/149‑1 (MQ=255)                                                                      tCACTATCACCGACTAAACGGCCATCCAGAGTGAACGGTTTGC‑‑CAGGGTAGGTCGACTGCAACTGGGAGACGGCTGCGAGCAGCCCTTCAGATGCCTTTTGGATTTTCGCCTCGGTCTTCAGGTCAATCAAATCTCGCGATGCTCGCa                                                                               >  5:16539/1‑148 (MQ=255)                                                                      tCACTATCACCGACTAAACGGCCATCCAGAGTGAACGGTTTGC‑‑CAGGGTAGGTCGACTGCAACTGGGAGACGGCTGCGAGCAGCCCTTCAGATGCCTTTTGGATTTTCGCCTCGGTCTTCAGGTCAATCAAATCTCGCGATGCTCGCa                                                                               >  6:44886/1‑148 (MQ=255)                                                                      tCACTATCACCGACTAAACGGCCATCCAGAGTGAACGGTTTGC‑‑CAGGGTAGGTCGACTGCAACTGGGAGACGGCTGCGAGCAGCCCTTCAGATGCCTTTTGGATTTTCGCCTCGGTCTTCAGGTCAATCAAATCTCGCGATGCTCGCa                                                                               >  4:65461/1‑148 (MQ=255)                                                                      tCACTATCACCGACTAAACGGCCATCCAGAGTGAACGGTTTGC‑‑CAGGGTAGGTCGACTGCAACTGGGAGACGGCTGCGAGCAGCCCTTAAGATGCCTTTTGGATTTTCGCCTCGGTCTTCAGGTCAATCAAATCTCGCGATGCTCGCa                                                                               >  8:155428/1‑148 (MQ=255)                                                                       cACTATCACCGACTAAACGGCCATCCAGAGTGAACGGTTTGC‑‑CAGGGTAGGTCGACTGCAACTGGGAGACGGCTGCGAGCAGCCCTTCAGATGCCTTTTGGATTTTCGCCTCGGTCTTCAGGTCAATCAAATCTCGCGATGCTCGCa                                                                               >  2:298614/1‑147 (MQ=255)                                                                        aCTATCACCGACTAAACGGCCATCCAGAGTGAACGGTTTTCCTCAGGGTAGGTCGACTGCAACTGGGAGACGGCTGCGAGCAGCCCTTCAGATGCCTTTTGGATTTTCGCCTCGGTCTTCAGGTCAATCAAATCTCGCGATGCTCGc                                                                                <  1:123774/147‑1 (MQ=255)                                                                          tATCACCGACTAAACGGCCATCCAGAGTGAACGGTTTGC‑‑CAGGGTAGGTCGACTGCAACTGGGAGACGGCTGCGAGCAGCCCTTCAGATGCCTTTTGGATTTTCGCCTCGGTCTTCAGGTCAATCAAATCTCGCGATGCTCGCATAGca                                                                          >  8:341620/1‑148 (MQ=255)                                                                              aCCGACTAAACGGCCATCCAGAGTGAACGGTTTGC‑‑CAGGGTAGGTCGACTGCAACTGGGAGACGGCTGCGAGCAGCCCTTCAGATGCCTTTTGGATTTTCGCCTCGGTCTTCAGGTCAATCAAATCTCGCGATGCTCGCATAGCCTTGc                                                                      >  7:288737/1‑149 (MQ=255)                                                                              aCCGACTAAACGGCCATCCAGAGTGAACGGTTTGC‑‑CAGGGTAGGTCGACTGCAACTGGGAGACGGCTGCGAGCAGCCCTTCAGATGCCTTTTGGATTTTCGCCTCGGTCTTCAGGTCAATCAAATCTCGCGATGCTCGCATAGCCTTGc                                                                      >  6:127157/1‑149 (MQ=255)                                                                               ccGACTAAACGGCCATCCAGAGTGAACGGTTTGC‑‑CAGGGTAGGTCGACTGCAACTGGGAGACGGCTGCGAGCAGCCCTTCAGATGCCTTTTGGATTTTCGCCTCGGTCTTCAGGTCAATCAAATCTCGCGATGCTCGCATAGCCTTGcc                                                                     <  3:217889/149‑1 (MQ=255)                                                                               ccGACTAAACGGCCATCCAGAGTGAACGGTTTGC‑‑CAGGGTAGGTCGACTGCAACTGGGAGACGGCTGCGAGCAGCCCTTCAGATGCCTTTTGGATTTTCGCCTCGGTCTTCAGGTCAATCAAATCTCGCGATGCTCGCATAGCCTTGcc                                                                     >  6:78284/1‑149 (MQ=255)                                                                                cGACTAAACGGCCATCCAGAGTGAACGGTTTGC‑‑CAGGGTAGGTCGACTGCAACTGGGAGACGGCTGCGAGCAGCCCTTCAGATGCCTTTTGGATTTTCGCCTCGGTCTTCAGGTCAATCAAATCTCGCGATGCTCGCATAGCCTTGccc                                                                    <  6:16539/149‑1 (MQ=255)                                                                                 gACTAAACGGCCATCCAGAGTGAACGGTTTGC‑‑CAGGGTAGGTCGACTGCAACTGGGAGACGGCTGCGAGCAGCCCTTCAGATGCCTTTTGGATTTTCGCCTCGGTCTTCAGGTCAATCAAATCTCGCGATGCTCGCATAGCCTTGCCCt                                                                   <  5:127157/149‑1 (MQ=255)                                                                                   cTAAACGGCCATCCAGAGTGAACGGTTTGC‑‑CAGGGTAGGTCGACTGCAACTGGGAGACGGCTGCGAGCAGCCCTTCAGATGCCTTTTGGATTTTCGCCTCGGTCTTCAGGTCAATCAAATCTCGCGATGCTCGCATAGCCTTGCCCTAt                                                                 <  4:37822/149‑1 (MQ=255)                                                                                        cGGCCATCCAGAGTGAACGGTTTGC‑‑CAGGGTAGGTCGACTGCAACTGGGAGACGGCTGCGAGCAGCCCTTCAGATGCCTTTTGGATTTTCGCCTCGGTCTTCAGGTCAATCAAATCTCGCGATGCTCGCATAGCCTTGCCCTATGACCg                                                            <  3:65461/149‑1 (MQ=255)                                                                                         ggCCATCCAGAGTGAACGGTTTGC‑‑CAGGGTAGGTCGACTGCAACTGGGAGACGGCTGCGAGCAGCCCTTCAGATGCCTTTTGGATTTTCGCCTCGGTCTTCAGGTCAATCAAATCTCGCGATGCTCGCATAGCCTTGCCCTATGACCgg                                                           <  6:283965/149‑1 (MQ=255)                                                                                                 agagTGAACGGTTTGC‑‑CAGGGTAGGTCGACTGCAACTGGGAGACGGCTGCGAGCAGCCCTTCAGATGCCTTTTGGATTTTCGCCTCGGTCTTCAGGTCAATCAAATCTCGCGATGCTCGCATAGCCTTGCCCTATGACCGGCTACATCa                                                   <  1:298614/149‑1 (MQ=255)                                                                                                  gagTGAACGGTTTGC‑‑CAGGGTAGGTCGACTGCAACTGGGAGACGGCTGCGAGCAGCCCTTCAGATGCCTTTTGGATTTTCGCCTCGGTCTTCAGGTCAATCAAATCTCGCGATGCTCGCATAGCCTTGCCCTATGACCGGCTACAt                                                     >  4:20264/1‑146 (MQ=255)                                                                                                       aaCGGTTTGC‑‑CAGGGTAGGTCGACTGCAACTGGGAGACGGCTGCGAGCAGCCCTTCAGATGCCTTTTGGATTTTCGCCTCGGTCTTCAGGTCAATCAAATCTCGCGATGCTCGCATAGCCTTGCCCAATGACCGGCTACAACACTATCt                                             >  7:54414/1‑149 (MQ=255)                                                                                                         cGGTTTGC‑‑CAGGGTAGGTCGACTGCAACTGGGAGACGGCTGCGAGCAGCCCTTCAGATGCCTTTTGGATTTTCGCCTCGGTCTTCAGGTCAATCAAATCTCGCGATGCTCGCATAGCCTTGCCCTATGACCGGCTACATCAGAATCtt                                            <  3:13493/148‑1 (MQ=255)                                                                                                         cGGTTTGC‑‑CAGGGTAGGTCGACTGCAACTGGGAGACGGCTGCGAGCAGCCCTTCAGATGCCTTTTGGATTTTCGCCTCGGTCTTCAGGTCAATCAAATCTCGCGATGCTCGCATAGCCTTGCCCTATGACCGGCTACATCAGAATCTTg                                           >  2:115321/1‑149 (MQ=255)                                                                                                          ggTTTGC‑‑CAGGGTAGGTCGACTGGAACTGGGAGACGGCTGCGAGCAGCCCTTCAGATGCCTTTTGGATTTTCGCCTCGGTCTTCAGGTCAATCAAATCTCGCGATGCTCGCATAGCCTTGCCCTATGACCGGCTACATCAGAATCTTGt                                          <  7:155428/149‑1 (MQ=255)                                                                                                          ggTTTGC‑‑CAGGGTAGGTCGACTGCAACTGGGAGACGGCTGCGAGCAGCCCTTCAGATGCCTTTTGGATTTTCGCCTCGGTCTTCAGGTCAATCAAATCTCGCGATGCTCGCATAGCCTTGCCCTATGACCGGCTACATCAGAATCTTGt                                          <  5:44886/149‑1 (MQ=255)                                                                                                              tGC‑‑CAGGGTAGGTCGACTGCAACTGGGAGACGGCTGCGAGCAGCCCTTCAGATGCCTTTTGGATTTTCGCCTCGGTCTTCAGGTCAATCAAATCTCGCGATGCTCGCATAGCCTTGCCCTATGACCGGCTACATCAGAATCTTGtttt                                       <  6:70136/148‑1 (MQ=255)                                                                                                               gC‑‑CAGGGTAGGTCGACTGCAACTGGGAGACGGCTGCGAGCAGCCCTTCAGATGCCTTTTGGATTTTCGCCTCGGTCTTCAGGTCAATCAAATCTCGCGATGCTCGCATAGCCTTGCCCTATGACCGGCTACATCAGAATCTTGTTTTc                                      >  7:299277/1‑148 (MQ=255)                                                                                                                                 tGCAACTGGGAGACGGCTGCGAGCAGCCCTTCAGATGCCTTTTGGATTTTCGCCTCGGTCTTCAGGTCAATCAAATCTCGCGATGCTCGCATAGCCTTGCCCTATGACCGGCTACATCAGAAt                                               >  4:344018/1‑123 (MQ=255)                                                                                                                                 tGCAACTGGGAGACGGCTGCGAGCAGCCCTTCAGATGCCTTTTGGATTTTCGCCTCGGTCTTCAGGTCAATCAAATCTCGCGATGCTCGCATAGCCTTGCCCTATGACCGGCTACATCAGAAt                                               <  3:344018/123‑1 (MQ=255)                                                                                                                                   cAACTGGGAGACGGCTGCGAGCAGCCCTTCAGATGCCTTTTGGATTTTCGCCTCGGTCTTCAGGTCAATCAAATCTCGCGATGCTCGCATAGCCTTGCCCTATGACCGGCTACATCAGAATCTTGTTTTCACTTCAATAACCTTGCCa                    <  7:341620/148‑1 (MQ=255)                                                                                                                                    aaCTGGGAGACGGCTGCGAGCAGCCCTTCAGATGCCTTTTGGATTTTCGCCTCGGTCTTCAGGTCAATCAAATCTCGCGATGCTCGCATAGCCTTGCCCTATGACCGGCTACATCAGAATCTTGTTTTCACTTCAATAACCTTGCCATg                  <  2:240706/149‑1 (MQ=255)                                                                                                                                         ggAGACGGCTGCGAGCAGCCCTTCAGATGCCTTTTGGATTTTCGCCTCGGTCTTCAGGTCAATCAAATCTCGCGATGCTCGCATAGCCTTGCCCTATGACCGGCTACATCAGAATCTTGTTTTCACTTCAATAACCTTGCCATGCAAg              <  8:299277/148‑1 (MQ=255)                                                                                                                                         ggAGACGGCTGCGAGCAGCCCTTCAGATGCCTTTTGGATTTTCGCCTCGGTCTTCAGGTCAATCAAATCTCGCGATGCTCGCATAGCCTTGCCCTATGACCGGCTACATCAGAATCTTGTTTTCACTTCAATAACCTTGCCATGCAAg              >  4:101077/1‑148 (MQ=255)                                                                                                                                          tgaagggCTGCTCGCAGCCCTTCAGATGCCTTTTGGATTTTCGCCTCGGTCTTCAGGTCAATCAAATCTCGCGATGCTCGCATAGCCTTGCCCTATGACCGGCTACATCAGAATCTTGTTTTCACTTCAATAACCTTGCCATGCAAg              >  4:330040/6‑147 (MQ=255)                                                                                                                                           agaCGGCTGCGAGCAGCCCTTCAGATGCCTTTTGGATTTTCGCCTCGGTCTTCAGGTCAATCAAATCTCGCGATGCTCGCATAGCCTTGCCCTATGACCGGCTACATCAGAATCTTGTTTTCACTTCAATAACCTTGCCATGCAAGCAg           <  3:209074/149‑1 (MQ=255)                                                                                                                                            aagggCTGCTCGCAGCCCTTCAGATGCCTTTTGGATTTTCGCCTCGGTCTTCAGGTCAATCAAATCTCGCGATGCTCGCATAGCCTTGCCCTATGACCGGCTACATCAGAATCTTGTTTTCACTTCAATAACCTTGCCATGc                 >  2:132409/4‑142 (MQ=255)                                                                                                                                                  tGCGAGCAGCCCTTCAGATGCCTTTTGGATTTTCGCCTCGGTCTTCAGGTCAATCAAATCTCGCGATGCTCGCATAGCCTTGCCCTATGACCGGCTACATCAGAATCTTGTTTTCACTTCAATAACCTTGCCATGCAAGCAGTATCAtt    >  3:177436/1‑149 (MQ=255)                                                                                                                                                  tGCGAGCAGCCCTTCAGATGCCTTTTGGATTTTCGCCTCGGTCTTCAGGTCAATCAAATCTCGCGATGCTCGCATAGCCTTGCCCTATGACCGGCTACATCAGAATCTTGTTTTCACTTCAATAACCTTGCCATGCAAGCAGTATCAtt    <  3:20264/149‑1 (MQ=255)                                                                                                                                                    cGAGCAGCCCTTCAGATTCCTTTTGGATTTTCGCCTCGGTCTTCAGGTCAATCAAATCTCGCGATGCTCGCATAGCCTTGCCCTATGACCGGCTACATCAGAATCTTGTTTTCACTTCAATAACCTTGCCATGCAAGCAGTATCATTGa  <  8:211265/149‑1 (MQ=255)                                                                                                                                                     |                                                                                                                                                    ACCGCATCAGGGAGCTTCGTCAGCCCTTCGTTCTGCGTCAACTTGTAAGCTAGGCTGGCAACCACTTCCTCACTATCACCGACTAAACGGCCATCCAGAGTGAACGGTTTGC‑‑CAGGGTAGGTCGACTGCAACTGGGAGACGGCTGCGAGCAGCCCTTCAGATGCCTTTTGGATTTTCGCCTCGGTCTTCAGGTCAATCAAATCTCGCGATGCTCGCATAGCCTTGCCCTATGACCGGCTACATCAGAATCTTGTTTTCACTTCAATAACCTTGCCATGCAAGCAGTATCATTGA  >  NC_002947/5391678‑5391971

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑